CN203187681U - Germ or cell full-gas direct exposure device - Google Patents
Germ or cell full-gas direct exposure device Download PDFInfo
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- CN203187681U CN203187681U CN 201320148355 CN201320148355U CN203187681U CN 203187681 U CN203187681 U CN 203187681U CN 201320148355 CN201320148355 CN 201320148355 CN 201320148355 U CN201320148355 U CN 201320148355U CN 203187681 U CN203187681 U CN 203187681U
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Abstract
The utility model discloses a germ or cell full-gas direct exposure device, and belongs to the technical field of gas contamination cultivation. The germ or cell full-gas direct exposure device consists a pure air generator (4) connected with an air inlet (3) of a germ or cell full-gas direct exposure instrument (1) through a polyurethane (PU) transparent pipe (5) and a linear smoke sucker (10) connected with an exposure bottle (12) of the germ or cell full-gas direct exposure instrument (1) through a rubber pipe (9) and a belt filter rubber pipe (11), wherein the germ or cell full-gas direct exposure instrument (1) mainly comprises a case shell, the exposure bottle (12), a gas flow control system, a constant-temperature heating system, a control system and an exposure bottle bracket (8). The germ or cell full-gas direct exposure device has the characteristics of simple structure, convenience in operation, high stability, low cost and capability of being connected to the current linear smoke sucker and can directly expose germs or cells in the exposure bottle and full gas at the same time and execute terminal point detection for different biological effects.
Description
Technical field:
The utility model relates to a kind of bacterium or the direct exposing device of the full flue gas of cell, belongs to gas exposure culture technique field.
Background technology:
The external toxicology of order first three items detects the safety evaluation that (Ames test (Salmonella reversion test), vitro cell micronucleus test and toluylene red cell toxicity test) has been widely used in cigarette smoke.In these detect, all relate to capture and the preparation of flue gas sample.Classical sample capture and the method for preparation last detect to as if the cambridge filter of dimethyl sulfoxide (DMSO) extraction on the flue gas total particulate matter.But this method reckons without the gaseous component of flue gas, the interaction between granule phase substance, gas phase thing and volatile matter; Therefore full fume exposure method is arisen at the historic moment.In full fume exposure method, cigarette smoke all directly is passed into to cultivate remains in the exposure container of bacterial detection or cell, the cigarette smoke of this moment is in " original " state (native) relatively, relatively the situation of human body cell contact flue gas during anthropomorphic dummy's smoking more can truly reflect bacterium or the situation of cellular exposure in full smoke components.
Commercial full fume exposure device has the Cultex[1 of Japan] and German Vitrocell[2].These two kinds of equipment are formed by smoking machine, flue gas dilution distribution system, substratum supply system, substratum temperature maintenance system and bacterium or five parts of cellular exposure device.When being used for cellular exposure, with cell seeding at transwell cell (porous transwell
TM) bottom film on, cell bottom contact nutrient solution is realized the cellular exposure of the full flue gas of liquid-vapo(u)r interface contact.When being used for the bacterial exposure of Salmonella reversion test, principle of device is similar during to cellular exposure with device construction, just with the transwell cell of cultivating germy culture dish vicarious species explant cell, directly culture dish is placed the fume exposure chamber to realize the full fume exposure [3] of bacterium.This device can be realized the cellular exposure of the full flue gas of liquid-vapo(u)r interface contact and the full fume exposure of bacterium, carry out the full flue gas analysis of cigarette and environmental gas analysis, but this type of exposing device is expensive, connects complexity, complicated operation, stable bad.
British American Tobacco and Imperial Tobacco Group have designed and produced full fume exposure device [3,4] voluntarily in addition, the full fume exposure device of British American Tobacco has comprised flue gas recirculation system and bacterium or cell culture system, the transwell cell has been adopted in cultivation to cell equally, realizes the cellular exposure of the full flue gas of liquid-vapo(u)r interface contact.The 96 porocyte culture plates that cell directly will have been cultivated by Imperial Tobacco Group carry out full fume exposure, and flue gas leads in each culture hole by the mode of similar flue gas injection, and what this device was realized is the full fume exposure of solution-air contact.The exposing device of British American Tobacco and Imperial Tobacco Group's design can reach full fume exposure experimental requirements, but only limits to themselves patent equipment, and the correlative study report is not seen in not commercialization yet.
In addition, U.S. Battelle company has made easy full fume exposure device with the Tissue Culture Flask repacking, cell attachment is incubated in the culturing bottle, and flue gas feeds in the culturing bottle, and the certain angle of vibration makes to flow about nutrient solution in the culturing bottle and realizes the full fume exposure of cell about vibrator.In this exposure chamber, flue gas is not direct fully and cells contacting, and it is residual to have the part nutrient solution, thereby flue gas has part in nutrient solution, and this process for exposing is between the exposure chamber of the device of Cultex and Imperial Tobacco Group's exploitation.Domestic full fume exposure device " be used for the device ZL 200720092154.3 that the external toxicology main flume of cigarette exposes " and carry out improved " the full fume exposure contamination device of cigarette CN 201560188 U " on this basis and U.S. Battelle company similar.What " a kind of device ZL201120063251.6 for the full fume exposure of cigarette " realized is the full fume exposure of solution-air contact of bacterium or cell.
As mentioned above, the Vitrocell of the Cultex of present Japan, Germany and British American Tobacco's full fume exposure device of designing voluntarily and making can realize that all the liquid-vapo(u)r interface contact of cell exposes and the full fume exposure of bacterium.The full fume exposure device that Imperial Tobacco Group designs voluntarily and makes can be realized the full fume exposure of solution-air contact of cell." a kind of device ZL201120063251.6 for the full fume exposure of cigarette " can realize the full fume exposure of solution-air contact of bacterium or cell.But present commercial the have only Cultex of Japan and the Vitrocell of Germany, their device is expensive, connects complexity, complicated operation, stable bad; And British American Tobacco and Imperial Tobacco Group design voluntarily and the full fume exposure device made is its oneself patent equipment, and not commercialization does not see that at present other research institutions use this equipment to carry out full fume exposure experimental study yet.
By literature search, do not see the open report identical with the present invention.
Reference:
[1] Cultex Laboratories. http://www.cultex.com/.
[2] Vitrocell Systems GmbH. http://www.vitrocell.com/.
[3] Aufderheide M, Gressmann H. A modified Ames assay reveals the mutagenicity of native cigarette mainstream smoke and its gas vapour phase [J]. Experimental and Toxicologic Pathology, 2007, 58(6): 383-392.
[4] Gaca M, Phillips J, Thorne d, et al. A new system for measuring the toxicity of mainstream cigarette smoke [C]. 2005 Coresta Congress, 2005.
[5] Coresta in vitro toxicology task force. In vitro exposure of cells to smoke at the air liquid interface [EB/OL]. http:///www.CORESTA.org.
Summary of the invention:
The purpose of this utility model is to overcome the expensive deficiency of prior art, and provides a kind of simple in structure, and is easy to operate, good stability, and cost is low, the bacterium that can be connected with existing linear pattern smoking machine or the direct exposing device of the full flue gas of cell.
The direct exposing device of the full flue gas of bacterium of the present utility model or cell, directly expose instrument (1) by bacterium or the full flue gas of cell, directly expose the pure air producer (4) that the inlet mouth (3) of instrument (1) is connected by PU transparent tube (5) and bacterium or the full flue gas of cell, and linear pattern smoking machine (10) composition that is connected by rubber tubing (9) and the exposure bottle (12) of being with filter rubber tubing (11) and bacterium or the full flue gas of cell directly to expose instrument (1).
The full flue gas of described bacterium or cell directly exposes the exposure bottle rack (8) that instrument (1) mainly comprises casing, exposure bottle (12), gas flow Controlling System, thermostatically heating system, Controlling System and is positioned at the casing side; Wherein: casing is made of cabinet outer cover (17), inlet mouth (3), louvre (15) and fan (16); The gas flow Controlling System is made of gas diverter assembly (2), gas storage tank (6), flow control meter assembly (7); The thermostatically heating system is made of Water Tank with Temp.-controlled (13); Controlling System is made of the cruel general of panel computer-M7(14).
Exposure bottle of the present utility model (12) selects for use patent " the full fume exposure device of cell liquid-gas interface contact cigarette application number: 201210171016.X " and " a kind of device ZL201120063251.6 for the full fume exposure of cigarette " to expose bottle; Gas storage tank assembly (6), flow control meter assembly (7), Water Tank with Temp.-controlled (13), the cruel general of panel computer-M7(14) and fan (15), pure air producer (3) are bought by market; Linear pattern smoking machine (9) is bought from Britain CERULEAN company.All the other are processed into according to a conventional method certainly for the standard material that adopts market sale.
The gas diverter assembly is stored in five road gases in the gas-holder (6) stably; Gas storage tank (6) links to each other with stream flow control meter assembly (7), realizes the flow control of five road air; Air flow quantity in the flow control meter assembly (7) is controlled by panel computer (14); Stream flow control meter assembly (7) exposes bottle with the air of different volumes by the air intlet input that exposes bottle (12); Mixing back flue gas air links to each other with straight line smoking machine (4) by the rubber tubing (11) that connects filter; The gas approach that exposes bottle (12) closely is connected by rubber tubing (9) with the linear pattern smoking machine, realizes that the flue gas input exposes bottle.
The utlity model has simple in structure, easy to operate, good stability, the characteristics that cost is low, can connect existing linear pattern smoking machine, can realize bacterium or cell and the directly exposure simultaneously of full flue gas in a plurality of exposure bottles, and after bacterium or cell directly contact the full smoke components of different concns, can carry out the end point determination of different biological effect.
Description of drawings:
Fig. 1 is connection diagram of the present utility model.
Fig. 2 is the structural representation that the full flue gas of bacterium or cell directly exposes instrument (1).
Fig. 3 is the connection diagram that the full flue gas of bacterium or cell directly exposes instrument (1).
Fig. 4 is the electrical structure synoptic diagram that the full flue gas of bacterium or cell directly exposes instrument (1) control device part.
Fig. 5 is the side diagram that the full flue gas of bacterium or cell directly exposes the gas diverter assembly (2) of instrument (1).
Embodiment:
The direct exposing device of the full flue gas of bacterium of the present utility model or cell, directly expose instrument 1 by bacterium or the full flue gas of cell, directly expose the pure air producer 4 that the inlet mouth 3 of instrument 1 is connected by PU transparent tube 5 and bacterium or the full flue gas of cell, and directly expose linear pattern smoking machine 10 compositions that the exposure bottle 12 of instrument 1 is connected by rubber tubing 9 and band filter rubber tubing 11 and bacterium or the full flue gas of cell.
The full flue gas of described bacterium or cell directly exposes the exposure bottle rack 8 that instrument 1 mainly comprises casing, exposure bottle 12, gas flow Controlling System, thermostatically heating system, Controlling System and is positioned at the casing side; Wherein: casing is made of cabinet outer cover 17, inlet mouth 3, louvre 15 and fan 16; The gas flow Controlling System is made of gas diverter assembly 2, gas storage tank 6, flow control meter assembly 7; The thermostatically heating system is made of Water Tank with Temp.-controlled 13; Controlling System is made of the cruel general-M714 of panel computer.
The gas diverter assembly is stored in five road gases in the gas-holder 6 stably; Gas storage tank 6 links to each other with stream flow control meter assembly 7, realizes the flow control of five road air; Air flow quantity in the flow control meter assembly 7 is by panel computer (cruel general-M7) 14 controls; Stream flow control meter assembly 7 exposes bottle with the air of different volumes by the air intlet input that exposes bottle 12; Mixing back flue gas air links to each other with straight line smoking machine 4 by the rubber tubing 11 that connects filter; The gas approach that exposes bottle 12 closely is connected by rubber tubing 9 with the linear pattern smoking machine, realizes that the flue gas input exposes bottle.
The concrete use of the direct exposing device of the full flue gas of this bacterium or cell is as follows:
1. check that bacterium or the full flue gas of cell directly expose the situation that is connected of inlet mouth 3 with the pure air producer 4 of instrument;
2. open the power supply that the full flue gas of bacterium/cell directly exposes instrument, press down the purging valve of pure air producer 4, discharge and tested entrap bubble last time;
3. (cruel general-M7) 14 sets the temperature of thermostatically heating grooves 13 directly to expose the panel computer of instrument at the full flue gas of bacterium/cell;
4. (cruel general-M7) 14 sets the air flow quantity of pure air producers 4 at panel computer;
5. cell to be detected and substratum or bacterium or cell suspending liquid are placed to expose bottle 12, will expose bottles 12 afterwards and place thermostatically heating groove 13;
6. the air that the full flue gas of bacterium/cell is directly exposed flow control meter assembly 7 derivation of instrument closely links to each other with the air intlet that exposes bottle 12;
6. straight line smoking machine flue gas incoming road sebific duct 9 closely is connected on the smoke inlet that exposes bottle;
7. rubber tubing 11 1 ends that will be connected with filter closely are connected to the smoke outlet that exposes bottle 12, and the other end closely is connected with the smoking machine loop;
8. set the automatic smoking machine program, the collection of beginning flue gas;
9. experiment finishes, and with the backward detaching device of erection sequence, deposits to carry out subsequent disposal with reaction system is appropriate.
Practical application shows that native system reaches design requirements fully.
Claims (2)
1. a bacterium or the direct exposing device of the full flue gas of cell, it is characterized in that device should directly expose instrument (1) by bacterium or the full flue gas of cell, directly expose the pure air producer (4) that the inlet mouth (3) of instrument (1) is connected by PU transparent tube (5) and bacterium or the full flue gas of cell, and linear pattern smoking machine (10) composition that is connected by rubber tubing (9) and the exposure bottle (12) of being with filter rubber tubing (11) and bacterium or the full flue gas of cell directly to expose instrument (1).
2. the bacterium of claim described 1 or the direct exposing device of the full flue gas of cell is characterized in that the full flue gas of bacterium or cell directly exposes the exposure bottle rack (8) that instrument (1) mainly comprises casing, exposure bottle (12), gas flow Controlling System, thermostatically heating system, Controlling System and is positioned at the casing side; Wherein: casing is made of cabinet outer cover (17), inlet mouth (3), louvre (15) and fan (16); The gas flow Controlling System is made of gas diverter assembly (2), gas storage tank (6), flow control meter assembly (7); The thermostatically heating system is made of Water Tank with Temp.-controlled (13); Controlling System is made of the cruel general of panel computer-M7(14).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320148355 CN203187681U (en) | 2013-03-28 | 2013-03-28 | Germ or cell full-gas direct exposure device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320148355 CN203187681U (en) | 2013-03-28 | 2013-03-28 | Germ or cell full-gas direct exposure device |
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| Publication Number | Publication Date |
|---|---|
| CN203187681U true CN203187681U (en) | 2013-09-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320148355 Expired - Lifetime CN203187681U (en) | 2013-03-28 | 2013-03-28 | Germ or cell full-gas direct exposure device |
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| CN (1) | CN203187681U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103146570A (en) * | 2013-03-28 | 2013-06-12 | 云南烟草科学研究院 | Direct exposure system of germ/cell flue gas |
-
2013
- 2013-03-28 CN CN 201320148355 patent/CN203187681U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103146570A (en) * | 2013-03-28 | 2013-06-12 | 云南烟草科学研究院 | Direct exposure system of germ/cell flue gas |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20130911 |