CN102653729A - 一种用于中国仓鼠卵巢细胞的培养基 - Google Patents
一种用于中国仓鼠卵巢细胞的培养基 Download PDFInfo
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- CN102653729A CN102653729A CN2011100519522A CN201110051952A CN102653729A CN 102653729 A CN102653729 A CN 102653729A CN 2011100519522 A CN2011100519522 A CN 2011100519522A CN 201110051952 A CN201110051952 A CN 201110051952A CN 102653729 A CN102653729 A CN 102653729A
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- chinese hamster
- hamster ovary
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Abstract
Description
成份 | 含量:g/L培养基 |
氯化钠 | 6-6.5 |
氯化钾 | 0.5-0.5 |
丙酮酸钠 | 0.462-1 |
硝酸钙 | 0.288-0.4 |
碳酸氢钠 | 1.464-2 |
D-葡萄糖 | 5.16-15 |
磷酸氢二钠 | 0.156-0.7 |
成份 | 含量:g/L培养基 |
L-丙氨酸 | 0.03738-0.218673 |
甘氨酸 | 0.0495-0.289575 |
L-异亮氨酸 | 0.3978-2.32713 |
L-色氨酸 | 0.090648-0.5302908 |
L-亮氨酸 | 0.58992-3.451032 |
L-天冬酰胺 | 0.62244-3.641274 |
L-苯丙氨酸 | 0.060432-0.3535272 |
L-酪氨酸 | 0.2328-1.36188 |
L-天冬氨酸 | 0.558-3.2643 |
L-谷氨酸 | 0.06174-0.361179 |
L-精氨酸 | 0.7734-4.52439 |
L-半胱氨酸 | 0.14748-0.862758 |
L-组氨酸 | 0.113232-0.6624072 |
L-脯氨酸 | 0.14496-0.848016 |
L-赖氨酸 | 2.4084-14.08914 |
L-丝氨酸 | 0.6684-3.91014 |
L-苏氨酸 | 0.9-5.265 |
L-蛋氨酸 | 0.1842-1.07757 |
L-缬氨酸 | 0.5268-3.08178 |
羟基L-脯氨酸 | 0.5-2.925 |
成份 | 含量:g/L培养基 |
硫酸镁 | 0.2748-1.60758 |
硫酸铁 | 0.516-3.0186 |
硫酸铜 | 0.00144-0.008424 |
氯化锰MnCl2 | 0.000005 0.00002925 |
硫酸镍,NiSO4 | 0.00000033-1.9305E-06 |
硅酸钠Na2SiO3 | 0.000284-0.0016614 |
偏钒酸钠NaVO3 | 0.00000146-0.000008541 |
钼酸钠NaMoO4 | 0.00000242-0.000014157 |
亚硒酸钠Na2SeO3 | 0.0000173-0.000101205 |
氯化亚锡SnCl2 | 0.00000028-0.000001638 |
硫酸锌ZnSO4 | 0.39564-2.314494 |
成份 | 含量:g/L培养基 |
4-羟乙基哌嗪乙磺酸(HEPES) | 4-6 |
普卢兰尼克F-68F(Pluronic F-68) | 1-2 |
亚油酸 | 0.0003528-0.00206388 |
酵母抽提物(DMV) | 3-10 |
成份 | 含量:g/L培养基 |
丙酮酸钠 | 5.67-10.773 |
硝酸钙 | 4.32-8.208 |
D-葡萄糖 | 100-190 |
磷酸氢二钠 | 3.24-6.156 |
成份 | 含量:g/L培养基 |
L-丙氨酸 | 0.5607-1.06533 |
甘氨酸 | 0.7425-1.41075 |
L-异亮氨酸 | 7.5-14.25 |
L-色氨酸 | 1.93-3.667 |
L-亮氨酸 | 8.8488-16.81272 |
L-天冬酰胺 | 9.3366-17.73954 |
L-苯丙氨酸 | 1.3-2.47 |
L-酪氨酸 | 4.3-8.17 |
L-天冬氨酸 | 8.37 15.903 |
L-谷氨酸 | 0.9261-1.75959 |
L-精氨酸 | 9-17.1 |
L-半胱氨酸 | 3-5.7 |
L-组氨酸 | 1.69848-3.227112 |
L-脯氨酸 | 1.2-2.28 |
L-赖氨酸 | 36.126-68.6394 |
L-丝氨酸 | 8-15.2 |
L-苏氨酸 | 13.5-25.65 |
L-蛋氨酸 | 4-7.6 |
L-缬氨酸 | 17-32.3 |
羟基L-脯氨酸 | 15-28.5 |
成份 | 含量:g/L培养基 |
硫酸镁 | 0.423-0.8037 |
硫酸铁 | 3.5-6.65 |
硫酸铜 | 0.0216-0.04104 |
氯化锰MnCl2 | 0.000075-0.0001425 |
硫酸镍NiSO4 | 0.00000495-0.000009405 |
硅酸钠Na2SiO3 | 0.00426-0.008094 |
偏钒酸钠NaVO3 | 0.0000219-0.00004161 |
钼酸钠NaMoO4 | 0.0000363-0.00006897 |
亚硒酸钠Na2SeO3 | 0.0002595-0.00049305 |
氯化亚锡SnCl2 | 0.0000042-0.00000798 |
硫酸锌ZnSO4 | 5.9346-11.27574 |
成份 | 含量:g/L培养基 |
亚油酸 | 0.005292-0.0100548 |
酵母抽提物 | 30-100 |
比较项目 | 实施例1的基础培养基 | JRH基础培养基 |
最高细胞密度(细胞/ml) | 5.3x106 | 3.9x106 |
最高细胞密度时活性 | 99.1% | 97.5% |
蛋白滴度(g/L) | 0.4 | 0.2 |
Claims (10)
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111454877A (zh) * | 2019-01-22 | 2020-07-28 | 鲁南制药集团股份有限公司 | 一种cho细胞培养方法 |
WO2024041228A1 (zh) * | 2022-08-25 | 2024-02-29 | 山东合成远景生物科技有限公司 | 一种生产聚唾液酸用的补料液及聚唾液酸制备方法 |
US11958894B2 (en) | 2019-12-06 | 2024-04-16 | Regeneron Pharmaceuticals, Inc. | Anti-VEGF protein compositions and methods for producing the same |
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CN101195816A (zh) * | 2007-12-28 | 2008-06-11 | 天津百若克医药生物技术有限责任公司 | 一种中国仓鼠卵巢细胞扩增培养基及其用途 |
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《Biotechnology letters》 20001231 zhaolie Chen "A low-cost chemically dedined protein free medium for a recombinant CHO cell line producing prothrombin" 837-841 摘要 表2 第22卷, * |
《海峡药学》 20061231 陈锋 "无血清细胞培养基的主要补充因子及其研究进展" 第12页 右栏 第1段 2-3,5-10 第18卷, 第4期 * |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111454877A (zh) * | 2019-01-22 | 2020-07-28 | 鲁南制药集团股份有限公司 | 一种cho细胞培养方法 |
CN111454877B (zh) * | 2019-01-22 | 2023-03-21 | 鲁南制药集团股份有限公司 | 一种cho细胞培养方法 |
US11958894B2 (en) | 2019-12-06 | 2024-04-16 | Regeneron Pharmaceuticals, Inc. | Anti-VEGF protein compositions and methods for producing the same |
US12012445B2 (en) | 2019-12-06 | 2024-06-18 | Regeneron Pharmaceuticals, Inc. | Anti-VEGF protein compositions and methods for producing the same |
US12012444B2 (en) | 2019-12-06 | 2024-06-18 | Regeneron Pharmaceuticals, Inc. | Anti-VEGF protein compositions and methods for producing the same |
WO2024041228A1 (zh) * | 2022-08-25 | 2024-02-29 | 山东合成远景生物科技有限公司 | 一种生产聚唾液酸用的补料液及聚唾液酸制备方法 |
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