CN102652762B - Application of effective parts of common andrographis herbs - Google Patents
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Abstract
The invention discloses the application of effective parts of common andrographis herbs in preparing medicaments with a cancer chemoprevention function. Because effective parts of common andrographis herbs can induce expression of quinone reductase so as to achieve a cancer chemoprevention effect, the effective part of common andrographis herbs can be used for preparing medicaments with a cancer chemoprevention function. According to the invention, effective parts of common andrographis herbs are prepared into a tablet form, and pharmacological experiments prove that the prepared tablet has a strong quinone reductase inducing activity, therefore, the tablet can achieve a better cancer chemoprevention effect. The method for preparing effective parts of common andrographis herbs comprises the following steps: carrying out extraction on all herbs or leaves of andrographis paniculata by using an extraction solvent so as to obtain an extracting solution; concentrating the extracting solution, adding water into the concentrated extracting solution to dissolve, carrying out extraction on the dissolved solution by using a extraction solvent, collecting extract liquor, and then concentrating the extract liquor so as to obtain the effective parts of common andrographis herbs with stable properties and a high pharmacological activity; and the method is simple in preparation process, easy to implement, and easy to operate and control, therefore, the method has extremely broad application prospects.
Description
Technical field
The present invention relates to extract application and the preparation field of effective site from Herba Andrographis, particularly a kind of application with the Herba Andrographis effective site of cancer chemoprevention effect.
Background technology
Global cancer statistical datas demonstrations in 2008 of international cancer research institution (IRAC) issue, within 2008, global cancer new cases are about 1,270 ten thousand, and cancer mortality number of cases is 7,600,000; Wherein the cancer new cases of developing country and death account for respectively 56% and 64%.February 4 in 2011, ， American Cancer Society (ACS) delivered global cancer statistical data report with regard to this statistical data.The Brawley of ACS time points out in comment, and in fact its risk factor of 35% cancer can be prevented, if taken effective measures, the cancer mortality case of 2008 2600000 (average every days approximately 7300 example) is avoidable.
This noun of cancer chemoprevention (Chemoprevention) was created by Michael Sporn in 1976, now confessed being defined as of national cancer institute (NCI) and other a plurality of mechanisms and individual utilized natural, synthetic or biological substance to stop, slow down or reverses cancer generation evolution, thereby reduce the methods and strategies of cancer incidence and mortality rate, so the prevention of tumor just seems particularly important.People can be by keeping good living habit (as non-smoking, do not indulge in excessive drinking etc.) to reach the object of the prevention of tumor, also can come prophylaxis of tumours to occur by the level of two-phase enzyme in rising body in addition, quinone reductase be exactly a kind of important phase II metabolic enzyme that in body, prophylaxis of tumours occurs.
Herba Andrographis be acanthaceous plant Herba Andrographis (circular cone must medicinal herbs, Andrographis paniculata (Burm.f.) Nees) go up dryly herb.This product stem is square column type, multi-branched, and the long 50～70cm of stem, joint slightly expands, and matter is crisp, frangibility.Single leaf is to life, the short or nearly stockless of petiole; Leaf-shrinkage, frangible, complete person is lanceolar or ovum shape lanceolar after launching, long 3～12cm, wide 2～5cm, tip is point gradually, and base portion wedge shape is downward, full edge or wavy; Upper surface is green, lower surface celadon, and two sides is smooth.Feeble QI, taste is extremely bitter.For heat-clearing and toxic substances removing, removing heat from blood, detumescence.For cold, fever, laryngopharynx swelling and pain, aphtha of the mouth and tongue, pertussis chronic cough, dysentery, the puckery pain of pyretic stranguria, carbuncle skin infection, venom.So far, in Herba Andrographis, the separated compound obtaining mostly is Diterpenes and flavonoid, and wherein the active component of report mostly is the derivant of andrographolide.Pharmacology's purposes of Herba Andrographis is very extensive, but up to now, there is not yet the report to its cancer chemoprevention effect.
Publication number is that the Chinese invention patent application of CN 101422494A discloses a kind of Herba Andrographis extract, described Herba Andrographis extract contains according to weight ratio: andrographolide 2% ~ 20%, dexyandrographolide 0.01% ~ 6%, 14-deoxidation-11,12 dehydrogenations-andrographolide 1% ~ 6%, neoandrographolide 1% ~ 6%, polysaccharide 18% ~ 28% and flavone compound 10% ~ 15%.Meanwhile, this application for a patent for invention also discloses a kind of extracting method of Herba Andrographis extract, and the ethanol with 80% ~ 95% extracts Herba Andrographis plant (particularly branch or the leaf of Herba Andrographis), then concentrates and get final product.The administering mode of this Herba Andrographis extract can be oral, rectally, injection, suction, implantation said extracted thing.Although proved and can be used for treating inflammatory bowel by pharmacological evaluation, the still unexposed chemoprophylaxis for cancer, is more difficult to see its drug effect.
Summary of the invention
The invention provides a kind of Herba Andrographis effective site and there is the application in the medicine of cancer chemoprevention effect in preparation.
Described Herba Andrographis effective site can induce quinone reductase to express, thereby plays the chemoprophylaxis effect of cancer.
Described cancer comprises cerebroma, pulmonary carcinoma, hepatocarcinoma, breast carcinoma, carcinoma of prostate, cancer of pancreas, cervical cancer, colon cancer, gastric cancer, esophageal carcinoma etc.
With mtt assay, carry out cancer chemoprevention effect screening, result demonstration, Herba Andrographis effective site of the present invention can significantly be induced the expression of quinone reductase.
Herba Andrographis effective site of the present invention can be combined and be prepared into medicine with commercially available or conventional carrier, for prophylaxis of tumours and cancer.Described medicine can be tablet or injection.
Herba Andrographis effective site of the present invention, when using (administration) in treatment, can provide different effects.Conventionally, Herba Andrographis effective site of the present invention can be formulated in to nontoxic, inertia with pharmaceutically acceptable aqueous carrier medium in, wherein, pH is generally 5 ~ 8, preferably pH is 6 ~ 8, and pH value can change to some extent with being formulated the character of material and disease to be treated.The medicine preparing can carry out administration by conventional route, comprising (but being not limited to): intramuscular, intraperitoneal, subcutaneous, Intradermal or topical.This class mounting medium comprises (but being not limited to): saline, buffer, glucose, water, glycerol, ethanol and combination thereof.The form of pharmaceutical preparation should match with administering mode.Herba Andrographis effective site of the present invention can be made into injection form, for example, with normal saline or the aqueous solution that contains glucose and other adjuvant, by conventional injection preparation method, be prepared.Medicine such as Tablet and Capsula, also can be prepared by conventional method.Medicine should be manufactured as injection solution, Tablet and Capsula under aseptic condition.Herba Andrographis effective site of the present invention also can be used together with other treatment agent.
When Herba Andrographis effective site of the present invention is used as medicine, the Herba Andrographis effective site of the present invention for the treatment of effective dose can be applied to mammal, wherein this treatment effective dose is at least 10 micrograms/kg body weight conventionally, and be in most of the cases no more than approximately 8 mg/kg body weight, preferably this dosage is 10 micrograms/kg body weight ~ 1 mg/kg body weight.Certainly, concrete dosage also should be considered the factors such as route of administration, patient health situation, and these are all within skilled practitioners skill.
Herba Andrographis effective site has the application in the medicine of cancer chemoprevention effect in preparation, and described Herba Andrographis effective site is made tablet, by the raw material of following weight portion, is made:
10 parts of Herba Andrographis effective sites;
3 ~ 7 parts of microcrystalline Cellulose;
3 ~ 7 parts of Sodium Hydroxymethyl Stalcses;
3 ~ 7 parts of polyvinylpyrrolidones;
3 ~ 7 parts of hyprolose;
1 ~ 3 part of magnesium stearate;
0.05 ~ 1 part of steviosin;
5 ~ 15 parts of calcium hydrogen phosphate.
As preferably, described Herba Andrographis effective site is made tablet, by the raw material of following weight portion, is made:
10 parts of Herba Andrographis effective sites;
5 parts of microcrystalline Cellulose;
5 parts of Sodium Hydroxymethyl Stalcses;
5 parts of polyvinylpyrrolidones;
5 parts of hyprolose;
2 parts of magnesium stearate;
0.3 part of steviosin;
10 parts of calcium hydrogen phosphate.
Polyvinylpyrrolidone can adopt the general trade mark of commercially available pharmaceutical grade polyvinylpyrrolidone.Hyprolose is selected the low replacement 2-hydroxypropyl ether cellulose that meets Chinese Pharmacopoeia 2010 regulations.
This method for preparing tablet thereof is as follows:
1) by Herba Andrographis effective site, microcrystalline Cellulose, hyprolose, calcium hydrogen phosphate and magnesium stearate, mix homogeneously, makes soft material with polyvinylpyrrolidone aqueous solution, dry rear granulate;
2) will in the powder of haveing suffered, add Sodium Hydroxymethyl Stalcs and steviosin, mix, tabletting, completes the preparation of Herba Andrographis effective fraction medicine tablet.
The tablet form of being made by the raw material of above-mentioned weight portion, analyzes by pharmacological evaluation, has very strong quinone reductase induced activity, can play well the chemoprophylaxis effect of cancer.
Described Herba Andrographis effective site is the extract that Herba Andrographis contains diterpene ginkgolide, mainly comprises andrographoside, andrographolide and 14-deoxidation-14,15-dehydrorographolide.
The preparation method of described Herba Andrographis effective site, it prepares simply, is easy to control, workable.
The preparation method of described Herba Andrographis effective site, comprises the following steps:
1) extract: use and extract solvent to medicinal material extract, obtain extracting solution;
Described medical material is herb or the leaf of Acanthaceae Herba Andrographis;
Described extraction solvent is the ethanol water containing ethanol mass percent 95%;
2) extract: the extracting solution that step 1) is obtained is concentrated, is dissolved in water, adopt extractant extraction, collect extract, after concentrating, obtain Herba Andrographis effective site.
In step 1), described extraction solvent is the ethanol water containing ethanol mass percent 95%.The character of this extraction solvent is very suitable for extracting herb or the leaf of Acanthaceae Herba Andrographis, can more fully obtain the herb of Acanthaceae Herba Andrographis or the active component in leaf, there is better extraction effect, and make the drug activity of the Herba Andrographis effective site that finally obtains higher.
Add the extraction solvent with respect to 6～10 times of weight of medical material weight, 90 ℃～100 ℃ heating extraction, each time of extracting is 2h～3h, extracts 2～3 times at every turn.Under such extraction conditions, not only saved the use amount of medical material and extraction solvent, and can fully the Herba Andrographis plant effective site in medical material have been extracted, improve extraction ratio.
Step 2), in, described extractant is a kind of or two or more in petroleum ether (60 ℃ ~ 90 ℃), normal hexane, cyclohexane extraction.As preferably, described extractant is the petroleum ether of 60 ℃ ~ 90 ℃ of boiling points.
The addition of described water is 0.1 ~ 0.5 times of medical material weight, is conducive to extractant and extracts.
The addition of described extractant is 0.2 ~ 0.6 times of medical material weight, is conducive to more fully obtain Herba Andrographis effective site.
Further preferably, each use the petroleum ether with respect to 60 ℃ ~ 90 ℃ of the boiling points of 0.13 times of medical material weight to extract, coextraction 3 times, collect extract, after concentrated, obtain Herba Andrographis effective site, under this condition, extract more complete, the Herba Andrographis plant effective site obtaining has very strong quinone reductase induced activity, the Herba Andrographis plant effective site obtaining can be made to the medicine with cancer chemoprevention effect.
Herba Andrographis effective site prepared by described preparation method, is the active constituents of medicine that derives from Herba Andrographis plant, is mainly diterpene ginkgolide.By liquid chromatography-mass spectrography (LC-MS/MS), analyze this Herba Andrographis effective site, identify three compound: a wherein, andrographoside; B, andrographolide; C, 14-deoxidation-14,15-dehydrorographolide.
A, andrographoside b, andrographolide c, 14-deoxidation-14,15-dehydrorographolide
Herba Andrographis effective site prepared by described preparation method has stable in properties, pharmacologically active advantages of higher, being applied preparation has in the medicine of cancer chemoprevention effect, medicine has very strong quinone reductase induced activity, can play well the chemoprophylaxis effect of cancer.
Compared with prior art, tool of the present invention has the following advantages:
Herba Andrographis effective site of the present invention can induce quinone reductase to express, thereby play the chemoprophylaxis effect of cancer, by Herba Andrographis effective site for the preparation of the medicine with cancer chemoprevention effect, the medicine of preparation has stronger quinone reductase induced activity, can play well the chemoprophylaxis effect of cancer.The present invention makes tablet form by Herba Andrographis effective site, and the tablet of making has proved to have very strong quinone reductase induced activity by pharmacological evaluation, can play well the chemoprophylaxis effect of cancer.
The preparation method of Herba Andrographis effective site of the present invention, preparation technology is simple, easy to implement, be easy to operate and control, thereby be conducive to large-scale industrial production, and the Herba Andrographis effective site of preparation has stable in properties, pharmacologically active advantages of higher, thereby there is boundless application prospect.
Accompanying drawing explanation
Fig. 1 is the high-efficient liquid phase chromatogram of the Herba Andrographis effective site of embodiment 1 preparation.
The specific embodiment
By specific embodiment, content of the present invention is described in further detail below, but embodiment should be interpreted as to limitation of the present invention.Without departing from the idea case in the present invention described above, various replacement means or the change according to ordinary skill knowledge and conventional means, made, within being all included in the present invention.
Embodiment 1
1) extract: Herba Andrographis herb, at 60 ℃ of dry 5h, is obtained to dry Herba Andrographis herb.Get the Herba Andrographis herb that 5Kg is dry, add the ethanol water (being ethanol water 30Kg) containing ethanol mass percent 95% with respect to 6 times of medical material weight at every turn, 90 ℃ of reflux, extract,, each time of extracting is 3h, altogether extract 3 times (ethanol water amounts to 90Kg) merge extractive liquid;
2) extraction: the extracting solution that step 1) is obtained is evaporated near dry, obtain thick extractum 450g, the water dissolution that adds 1Kg, then use the petroleum ether of 60 ℃ ~ 90 ℃ of 0.65Kg boiling points to extract at every turn, coextraction 3 times, collect extract, extract is concentrated and obtains Herba Andrographis effective site 200g.
Get the Herba Andrographis effective site 0.015g of embodiment 1 preparation, dissolve with methanol with 1mL, centrifugal, get supernatant and carry out Agilent 1100 HPLC analyses, liquid phase chromatogram condition: flow velocity: 0.5mL/min, uv absorption wavelength: 210nm, eluent system: acetonitrile-water, elution requirement: 0 ~ 90min, the mass percent of acetonitrile is 10% ~ 100%, its high performance liquid chromatography is as shown in Figure 1.Chromatographic peak by LC-MS analysis correspondence identifies wherein corresponding three compound as in the peak shown in Fig. 1, b, c:a, andrographoside; B, andrographolide; C, 14-deoxidation-14,15-dehydrorographolide.
A, andrographoside b, andrographolide c, 14-deoxidation-14,15-dehydrorographolide
Embodiment 2
1) extract: Herba Andrographis herb, at 60 ℃ of dry 5h, is obtained to dry Herba Andrographis herb.Get the Herba Andrographis herb that 5Kg is dry, add the ethanol water (being ethanol water 50Kg) containing ethanol mass percent 95% with respect to 10 times of medical material weight at every turn, 100 ℃ of reflux, extract,, each time of extracting is 2h, altogether extract 2 times (ethanol water amounts to 100Kg) merge extractive liquid;
2) extraction: the extracting solution that step 1) is obtained is evaporated near dry, obtain thick extractum 440g, the water dissolution that adds 0.5Kg, then use the petroleum ether of 60 ℃ ~ 90 ℃ of 0.35Kg boiling points to extract at every turn, coextraction 3 times, collect extract, extract is concentrated and obtains Herba Andrographis effective site 180g.
Chromatographic peak by LC-MS analysis correspondence identifies Herba Andrographis effective site comprising three compound as, b, c:a, andrographoside; B, andrographolide; C, 14-deoxidation-14,15-dehydrorographolide.
A, andrographoside b, andrographolide c, 14-deoxidation-14,15-dehydrorographolide
Embodiment 3
1) extract: Herba Andrographis herb, at 60 ℃ of dry 5h, is obtained to dry Herba Andrographis herb.Get the Herba Andrographis herb that 5Kg is dry, add the ethanol water (being ethanol water 40Kg) containing ethanol mass percent 95% with respect to 8 times of medical material weight at every turn, 90 ℃ of reflux, extract,, each time of extracting is 3h, altogether extract 3 times (ethanol water amounts to 120Kg) merge extractive liquid;
2) extraction: the extracting solution that step 1) is obtained is evaporated near dry, obtain thick extractum 470g, the water dissolution that adds 2Kg, then use the petroleum ether of 60 ℃ ~ 90 ℃ of 0.8Kg boiling points to extract at every turn, coextraction 3 times, collect extract, extract is concentrated and obtains Herba Andrographis effective site 220g.
Chromatographic peak by LC-MS analysis correspondence identifies Herba Andrographis effective site comprising three compound as, b, c:a, andrographoside; B, andrographolide; C, 14-deoxidation-14,15-dehydrorographolide.
A, andrographoside b, andrographolide c, 14-deoxidation-14,15-dehydrorographolide
The quinone reductase induced activity experiment of embodiment 4(Herba Andrographis effective site)
The take the logarithm Hepa 1c1c7 cell ，Mei hole 2 * 10 of phase
4be inoculated in the DMEM culture medium that 96 orifice plate Shang，Mei holes add 190 μ L, after 24h, abandon supernatant, press following grouping administration:
Administration matched group: by 4-bromine flavone dmso solution, be configured to the 4-bromine flavonoids solution of 4mg/ml, 6 Ge Fu Kong Zhongmei holes add the 4-bromine flavonoids solution of 10 μ L, and the concentration that makes to add 4-bromine flavone in 6 Ge Fu holes after 4-bromine flavonoids solution is 20 μ g/ml;
Herba Andrographis effective site dosing group: by the Herba Andrographis effective site dmso solution of embodiment 1 preparation, be configured to the Herba Andrographis effective site solution of 4mg/ml, 6 Ge Fu Kong Zhongmei holes add the Herba Andrographis effective site solution of 10 μ L, and the concentration that makes to add Herba Andrographis effective site in 6 Ge Fu holes after Herba Andrographis effective site solution is 20 μ g/ml;
Blank group: 6 Ge Fu Kong Zhongmei holes add 10 μ L dimethyl sulfoxide.
Administration matched group, Herba Andrographis effective site dosing group and blank group, establish 6 Ge Fu holes at every 96 orifice plates for every group, with two 96 orifice plate parallelly cultivates, cultivates after 24 hours, abandons supernatant, adds fresh medium 200.First block of plate surveyed cell survival rate with mtt assay, culture fluid (the tetrazolium bromide with MTT that adds 5mg/ml, green skies reagent company) 20 μ L, culture fluid with MTT is dissolved in phosphate buffer (PBS by MTT, pH=7.3) in, form, MTT concentration is 5mg/mL, after four hours, abandon culture fluid, add 150 μ L DMSO(dimethyl sulfoxide) vibrate 10 minutes, in microplate reader, 550nm place surveys OD value, cell survival rate=(administration group OD value-Blank)/(blank group OD value-Blank).Another piece plate adds digitonin 10 μ g to make lysis, then add culture fluid (0.5mg/ml) the 200 μ l with MTT to cultivate 5min, culture fluid with MTT is dissolved in phosphate buffer (PBS by MTT, pH=7.3) in, form, MTT concentration is 0.5mg/mL, in microplate reader, 550nm place surveys OD(optical density optical density) value, quinone reductase induction multiple IR=[(administration group OD value-Blank)/(blank group OD value-Blank)]/cell survival rate.Wherein, the cell survival rate during quinone reductase induction multiple calculates is first cell survival rate that plate calculates, and Blank refers to the intrinsic light absorption value of 96 orifice plate, and concrete outcome is as shown in table 1.
As shown in table 1, add after Herba Andrographis effective site of the present invention, medicine can significantly induce quinone reductase to express, and described cell is purchased from US mode culture collection warehousing (ATCC).
Table 1
The preparation of embodiment 5(Herba Andrographis effective fraction medicine tablet)
1) take Herba Andrographis effective site, the microcrystalline Cellulose of 0.025g, the calcium hydrogen phosphate of the hyprolose of 0.025g, 0.05g and the magnesium stearate of 0.01g of embodiment 1 preparation of 0.05g, mix homogeneously, with containing polyvinylpyrrolidone 0.025g polyvinylpyrrolidone aqueous solution, (polyvinylpyrrolidone adopts pharmaceutical grade K30, in polyvinylpyrrolidone aqueous solution, the mass percent of polyvinylpyrrolidone is 2%) make soft material, after being dried, cross 14 mesh sieve granulate.
2) will in the powder of haveing suffered, add the Sodium Hydroxymethyl Stalcs of 0.025g and the steviosin of 0.0015g, mix, tabletting, completes the preparation of Herba Andrographis effective fraction medicine tablet.
Above-mentioned Herba Andrographis effective site is replaced with to 4-bromine flavone (green the skies reagent company), repeating step 1) and step 2), 4-bromine flavone medicinal tablet obtained.
Above-mentioned Herba Andrographis effective site is got to 0g, repeating step 1) and step 2), adjuvant tablet obtained.
The quinone reductase induced activity experiment of embodiment 6(effective fraction medicine tablet)
The take the logarithm Hepa 1c1c7 cell ，Mei hole 2 * 10 of phase
4be inoculated in the DMEM culture medium that 96 orifice plate Shang，Mei holes add 190 μ L, after 24h, abandon supernatant, press following grouping administration:
Administration matched group: by the 4-bromine flavone tablet dmso solution of embodiment 5 preparations, be configured to the 4-bromine flavone sheet agent solution of 7mg/ml, 6 Ge Fu Kong Zhongmei holes add the 4-bromine flavone sheet agent solution ，Ji Mei hole of 10 μ L to add the 4-bromine flavone tablet of 70 μ g;
Dosing group: by the Herba Andrographis effective fraction medicine tablet dmso solution of embodiment 5 preparations, be configured to the Herba Andrographis effective fraction medicine sheet agent solution of 7mg/ml, 6 Ge Fu Kong Zhongmei holes add the Herba Andrographis effective fraction medicine sheet agent solution ，Ji Mei hole of 10 μ L to add the Herba Andrographis effective fraction medicine tablet of 70 μ g;
Adjuvant group: by the adjuvant tablet dmso solution of embodiment 5 preparations, be configured to the adjuvant sheet agent solution of 7mg/ml, 6 Ge Fu Kong Zhongmei holes add the adjuvant sheet agent solution ，Ji Mei hole of 10 μ L to add the adjuvant tablet of 70 μ g;
Blank group: 6 Ge Fu Kong Zhongmei holes add 10 μ L dimethyl sulfoxide.
Administration matched group, dosing group, adjuvant group and blank group, establish 6 Ge Fu holes at every 96 orifice plates for every group, with two 96 orifice plate parallelly cultivates, cultivates after 24 hours, abandons supernatant, adds fresh medium 200.First block of plate surveyed cell survival rate with mtt assay, culture fluid (the tetrazolium bromide with MTT that adds 5mg/ml, green skies reagent company) 20 μ L, culture fluid with MTT is dissolved in phosphate buffer (PBS by MTT, pH=7.3) in, form, MTT concentration is 5mg/mL, after four hours, abandon culture fluid, add 150 μ L DMSO(dimethyl sulfoxide) vibrate 10 minutes, in microplate reader, 550nm place surveys OD value, cell survival rate=(administration group OD value-Blank)/(blank group OD value-Blank).Another piece plate adds digitonin 10 μ g to make lysis, then add culture fluid (0.5mg/ml) the 200 μ l with MTT to cultivate 5min, culture fluid with MTT is dissolved in phosphate buffer (PBS by MTT, pH=7.3) in, form, MTT concentration is 0.5mg/mL, in microplate reader, 550nm place surveys OD(optical density optical density) value, quinone reductase induction multiple IR=[(administration group OD value-Blank)/(blank group OD value-Blank)]/cell survival rate.Wherein, the cell survival rate during quinone reductase induction multiple calculates is first cell survival rate that plate calculates, and Blank refers to the intrinsic light absorption value of 96 orifice plate, and concrete outcome is as shown in table 2.
As shown in table 2, add after Herba Andrographis effective fraction medicine tablet of the present invention, can significantly induce quinone reductase to express, described cell is purchased from US mode culture collection warehousing (ATCC).
Table 2
Claims (5)
1. a preparation method for Herba Andrographis effective site, is characterized in that, comprises the following steps:
1) extract: use and extract solvent to medicinal material extract, obtain extracting solution;
Described medical material is herb or the leaf of Acanthaceae Herba Andrographis;
Described extraction solvent is the ethanol water containing ethanol mass percent 95%;
Add the extraction solvent with respect to 6～10 times of weight of medical material weight, 90 ℃～100 ℃ heating extraction, each time of extracting is 2h～3h, extracts 2～3 times at every turn;
2) extract: the extracting solution that step 1) is obtained is concentrated, is dissolved in water, adopt extractant extraction, collect extract, after concentrating, obtain Herba Andrographis effective site;
Described extractant is the petroleum ether of 60 ℃～90 ℃ of boiling points;
Each use the petroleum ether with respect to 60 ℃～90 ℃ of the boiling points of 0.13 times of medical material weight to extract, coextraction 3 times;
Described Herba Andrographis effective site is the extract that Herba Andrographis contains diterpene ginkgolide, mainly comprises andrographoside a, andrographolide b and 14-deoxidation-14,15-dehydrorographolide c;
2. the preparation method of Herba Andrographis effective site as claimed in claim 1, is characterized in that step 2) in, the addition of described water is 0.1～0.5 times of medical material weight.
3. the Herba Andrographis effective site that prepared by preparation method as claimed in claim 1 or 2 has the application in the medicine of cancer chemoprevention effect in preparation, and described cancer is cerebroma, pulmonary carcinoma, hepatocarcinoma, breast carcinoma, carcinoma of prostate, cancer of pancreas, cervical cancer, colon cancer, gastric cancer and esophageal carcinoma.
4. Herba Andrographis effective site as claimed in claim 3 has the application in the medicine of cancer chemoprevention effect in preparation, it is characterized in that, described medicine is the tablet that utilizes Herba Andrographis effective site to make, and by the raw material of following weight portion, is made:
5. Herba Andrographis effective site as claimed in claim 4 has the application in the medicine of cancer chemoprevention effect in preparation, it is characterized in that, described medicine is the tablet that utilizes Herba Andrographis effective site to make, and by the raw material of following weight portion, is made:
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| CN100430054C (en) * | 2002-12-31 | 2008-11-05 | 北京大学第一医院 | Use of green chiretta diterpene lactone in inhibiting vascularization |
| CN1762347A (en) * | 2004-09-24 | 2006-04-26 | 贵阳云岩西创药物科技开发有限公司 | Andrographolide preparation and its production method |
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