CN102652758A - Application of health care drinking liquid containing deep seawater in preparation of medicaments or health care products for preventing or treating hyperlipidemia - Google Patents
Application of health care drinking liquid containing deep seawater in preparation of medicaments or health care products for preventing or treating hyperlipidemia Download PDFInfo
- Publication number
- CN102652758A CN102652758A CN2012101146035A CN201210114603A CN102652758A CN 102652758 A CN102652758 A CN 102652758A CN 2012101146035 A CN2012101146035 A CN 2012101146035A CN 201210114603 A CN201210114603 A CN 201210114603A CN 102652758 A CN102652758 A CN 102652758A
- Authority
- CN
- China
- Prior art keywords
- healht
- liquid
- care liquid
- deep seawater
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides an application of health care drinking liquid containing deep seawater in the preparation of medicaments or health care products for preventing or treating hyperlipoidemia. The health care drinking liquid comprises A liquid and B liquid, wherein the A liquid is of fresh water, the B liquid is of high-concentration concentrated liquid which is obtained by performing decompression concentration on a concentrated water part which is separated from the deep sweater via a reverse osmosis device, and the hardness range is 200-1000ppm. The health care drinking liquid is given to a rat with the hyperlipoidemia, which is subjected to modeling by a high-fat emulsion, in a free drinking way, after the medicament is administrated for 28 days, the blood lipid level of the rat with the hyperlipoidemia can be significantly reduced, lipid peroxidation can be inhibited, the activity of lipoprotein metabolic enzymes, namely LCAT (lecithin-cholesterol acetyltransferase), LPL (lipoprotein lipase) and HL (hepatic lipase), can be significantly improved, and the state of lipid metabolism disorder in the rat with the hyperlipoidemia, as well as concurrent fatty liver and diabetes thereof, can be further improved. The health care drinking liquid is derived from the deep seawater, has the advantages of abundant resources, easiness in industrialization and the like, and further has broad market and application prospects in the aspect of preventing and treating the hyperlipoidemia.
Description
Technical field
The invention belongs to the marine biotechnology field, relate to the comprehensive utilization of deep seawater, be specifically related to a kind of healht-care liquid that contains deep seawater in preparation prevention or the medicine of treatment hyperlipemia or the application in the health product.
Background technology
Improving constantly of Along with people's living standard, great variation has all taken place in life style and dietary structure, and the sickness rate of cardiovascular and cerebrovascular diseases such as hyperlipemia is also increasingly high.Hyperlipemia is called lipid metabolic disorder or unusual again, is meant that mainly TC, LDL-C and TG in the blood is higher than normal range and/or the HDL-C level is low excessively.Lipid metabolic disorder can cause that blood viscosity increases, slow blood flow, and lipidosis too much in the blood forms AP in the tremulous pulse inwall, causes stricture of artery, and blood flow is obstructed, and the target organ blood supply reduces and then forms damage.Brain arteriosclerosis can cause cerebral hemorrhage or cerebral thrombosis; The heart coronary artery sclerosis can cause uncomfortable in chest, nervous, and severe patient can cause myocardial infarction.In addition, hyperlipemia also is an important risk factor that promotes hypertension, impaired glucose tolerance, diabetes.Discover in the pathogeny of cardiovascular and cerebrovascular disease; Hyperlipemia is a main independent hazard factor; The serum lipids level descends 1%; Evidence of coronary heart diseases will reduce by 2%, so the generation of control hyperlipemia and development have great significance for the prevention cardiovascular and cerebrovascular disease, the prevention of hyperlipemia and treatment have become present research focus.
In the treatment of hyperlipemia, statins is used the most extensive, can effectively reduce the concentration of LDL-C in the blood, can reduce the incidence rate and the coronary heart disease death rate of cardiovascular accident incident behind the life-time service.But this medicine has certain side effect, such as liver function injury, rhabdomyolysis, myalgia, weak, erythra etc.; And blood fat gos up comparatively fast after the drug withdrawal, is difficult to reach the purpose of long-term treatment and prevention.
The adjustment dietary habit is the basis of the various hyperlipemias of treatment with the mode of making the life better, especially for idiopathic hyperlipidemia patient, and should first-selected Diet Therapy.People's such as Yang Weiguo research shows: along with the change of diet structure, patient's blood lipid level of 75% has decline in various degree.The decline of U.S.'s crowd's blood lipid level over nearly 20 years is relevant to the attention degree of Diet Therapy with doctor and patient.Therefore, research and development have the food of effect for reducing fat, thereby significant to improving hyperlipidemia through the adjustment diet structure.
Research shows; The generation of cardiovascular and cerebrovascular disease such as mineral element and hyperlipemia and develop closely related; Replenish useful mineral element magnesium, calcium, chromium, manganese, zinc, selenium through diet; Reduce the contact of poisonous trace element hydrargyrum, lead etc. simultaneously, to keep the relatively stable of mineral element content in the body, to the positive effect that has of cardiovascular and cerebrovascular diseases such as prophylaxis of hyperlipidemia.
Deep seawater typically refer to solar radiation less than, can not carry out photosynthesis, the sea water below about 200m degree of depth.Deep seawater is in the ocean of no sunlight entering does not have photosphere, and away from influence and pollution from the mankind, land and atmospheric chemical substance.Research shows that deep seawater has following main feature: (1) low temperature homeostasis-do not receive solar radiation, and changeable unlike the sea surface coolant-temperature gage, deep seawater is temperature-resistant all the year round, is constant at about 5 ℃; (2) the abundant and stability of mineral element-with sea surface water ratio; Deep seawater is rich in 92 kinds of mineral elements such as the necessary calcium of human body, magnesium, potassium, sodium, ferrum, copper, lithium, molybdenum, manganese, zinc, germanium, iodine, phosphorus, fluorine, selenium, trivalent chromium, silicate ion, has contained the required element kind of human body metabolism.Except its content is abundant; Because these water flow in the bathypelagic of " no photosphere " with the very long time; Ectocine with taking place, not receiving in unglazed cooperation, and therefore the composition of contained mineral element is very stable, has ideally solved the requirement that total balance of the body is absorbed various required mineral elements; (3) aseptic spatter property-the be in deep water of ocean " no photosphere " except away from the influence of human modern civilization and do not receive the pollution of land, Atmospheric Chemistry material, pathogenic bacteria, itself does not have the condition that generates antibacterials such as pathogen yet.Therefore, deep seawater is the water of the aseptic nature that cleans very much; (4) deep seawater water quality is alkalescence.
Owing to have above characteristics, deep seawater is receiving attracting attention of common people as a kind of new natural resources.Deep seawater is rich in various mineral and trace element, and it has good improvement effect to hyperlipemia in theory.China marine site is broad, and the coastline is very long, and marine resources are abundant, but does not still have the research of deep seawater Application and Development aspect; And China's water scarcity, water is seriously polluted, and the bathypelagic water resource of exploitation China has crucial meaning.
Summary of the invention
, water in short supply day by day to present China water resource pollutes and is on the rise and the rapid hot issue such as risings of hyperlipemia sickness rate, the invention provides a kind of healht-care liquid that contains deep seawater in the preparation prevention or treat the medicine of hyperlipemia or the application in the health product.
For realizing the foregoing invention purpose, the present invention adopts following technical proposals to be achieved:
A kind of healht-care liquid that contains deep seawater is in preparation prevention or the medicine of treatment hyperlipemia or the application in the health product; Said healht-care liquid comprises A liquid and B liquid, and said A liquid is fresh water, and said B liquid makes through following steps: deep seawater is isolated dense water section behind reverse osmosis unit; Dense water section is carried out concentrating under reduced pressure; Make its volume be concentrated into the 0.67-3.33% of original volume, remove by filter the sodium chloride of separating out then, the height concentrated solution that obtains is B liquid; The hardness of said healht-care liquid is at 200-1000ppm, and said deep seawater is below the 500m of sea level and the sea water after desalting processing.
Further improvement to technique scheme: magnesium in the said healht-care liquid: calcium: potassium: the concentration ratio of sodium element is 2.5-3.5: 0.45-1: 0.3-1: 0.3-1.5, do not contain Organic substance, and water quality is alkalescence.
Further improvement to technique scheme: said magnesium: calcium: the ratio of greater inequality of the concentration of potassium is 3: 1: 1.
Further improvement to technique scheme: the water quality of said healht-care liquid is alkalescence, the pH value 7.5 ± 0.3 of 25 ℃ of mensuration.
Further improvement to technique scheme: the beneficial element content in the said healht-care liquid is: zinc 0.0325-16.4 μ g/L; Selenium 0.0395-0.712 μ g/L; Chromium 0.104-4.54 μ g/L; Manganese 0.156-5.52 μ g/L.
Further improvement to technique scheme: the harmful element content in the said healht-care liquid is: lead＜5 μ g/L; Hydrargyrum＜0.5 μ g/L; Arsenic＜5 μ g/L; Cadmium＜1 μ g/L.
Further improvement to technique scheme: said deep seawater is taken from apart from beyond the 20km of coastline.
Further improvement to technique scheme: said fresh water sources Yu Haiyang deep water is isolated fresh water after the reverse osmosis unit circulation.
Further improvement to technique scheme: it is the healht-care liquid of 600-1000ppm that diet is selected hardness for use to the less hyperlipidemia patient of the picked-up of mineral, is the healht-care liquid of 200-400ppm otherwise select hardness.
Compared with prior art, advantage of the present invention and good effect are:
The healht-care liquid that contains deep seawater provided by the invention is rich in healthy trace elements with household magnesium, calcium, vanadium, chromium, manganese, zinc, selenium; And poisonous trace element hydrargyrum is very low with plumbous content; This healht-care liquid is blood lipid reducing level and AI (LDL-C/HDL-C) significantly; Reduce the liver accumulation of lipid simultaneously; And significantly reduce fasting glucose content, point out this healht-care fluid power significantly to regulate body lipid metabolic disorder and concurrent fatty liver and diabetes thereof, metabolic diseases such as hyperlipemia are had prevention and therapeutical effect preferably.
The specific embodiment
Below in conjunction with the specific embodiment technical scheme of the present invention is done further detailed explanation.
Embodiment 1
One, the preparation of healht-care liquid
The method for preparing of healht-care liquid according to the invention is following:
1) bathypelagic water pretreatment: will take from apart from beyond the 20km of coastline; Sea level 500m puts into water tank with dark deep seawater, adopts filter to filter, and removes phytoplankton and microorganism in the sea water; And, further remove Organic substance through the active carbon filter filtration.
2) fresh water and dense separated form water: the deep seawater that obtains after the pretreatment is divided into the fresh water part through high-pressure pump entering reverse osmosis unit with deep seawater, and (fresh water part water quality is identical with pure water, or water quality is near pure water with dense water section; The concentration of various zwitterions is near 2 times of various ion concentrations in the former sea water in the dense water section), isolated fresh water partly is that A liquid gets into the fresh water water tank, isolated dense water section gets into dense water water tank.The technological parameter of reverse osmosis unit is: 4MPa, power 1.5KW, aquifer yield 1T/d are pressed in running.
3) dense water takes off the sodium processing: the dense water section in the dense water water tank is carried out concentrating under reduced pressure; Make its volume be concentrated into the 0.67-3.33% of original volume; Remove by filter the sodium chloride of separating out then; Obtain highly spissated concentrated solution and be B liquid, magnesium in the said concentrated solution: calcium: potassium: the concentration ratio of sodium element is 2.5-3.5: 0.45-1: 0.3-1: 0.3-1.5, and said magnesium, calcium, potassium, the content concn unit of sodium element in concentrated solution are mg/L.Trace element chromium wherein, zinc, selenium, the concentration of manganese etc. and cycles of concentration are the multiple proportions relation.
Perhaps, also can adopt the selectivity electrodialysis to reverse osmosis after isolated dense water section take off sodium and handle, electrodialysis plant adopts 1 valency cation selective to see through film, cavity block adopts the conventional ion exchange membrane.All aniones all can see through cavity block in electrodialytic process, and only univalent cation sees through anode membrane, and the polyvalent cation that takes off in the sodium dense water afterwards is close with the concentration in the former dense water.
4) healht-care liquid fill: will pass through fresh water A liquid that the step 1) reverse osmosis unit comes out and the resulting B liquid of step 3) can according to demand mixed the healht-care liquid of different hardness, said hardness is with CaCO
3Cubage (GB 5749-2006 drinking water sanitary standard), hardness=Ca (ppm)) * 2.5+Mg (ppm) * 4.1 its computing formula is:, through high temperature sterilize, packing gets final product.
Adopt inductively coupled plasma mass spectrum (ICP-MS) that the inorganic element content in the healht-care liquid is measured: with hardness is that 1000 healht-care liquid is example, and wherein several kinds of main mine materials and micronutrient levels are: magnesium: 200-224mg/L; Calcium: 33-67mg/L; Potassium: 20-67mg/L; Sodium: 20-100mg/L (content of sodium is low more good more); Zinc: 0.0325-16.4 μ g/L; Selenium: 0.0395-0.712 μ g/L; Chromium: 0.104-4.54 μ g/L; Manganese 0.156-5.52 μ g/L, lead＜5 μ g/L; Hydrargyrum＜0.5 μ g/L; Arsenic＜5 μ g/L; Cadmium＜1 μ g/L.
Healht-care liquid mineral under the 200-1000 hardness according to the invention and micronutrient levels are directly proportional with its hardness.Magnesium in the said healht-care liquid: calcium: potassium: the concentration ratio of sodium element is 2.5-3.5: 0.45-1: 0.3-1: 0.3-1.5, is rich in healthy trace elements with household zinc, selenium, chromium; Manganese, poisonous trace element lead, hydrargyrum, arsenic; Cadmium content is lower, do not contain Organic substance, and water quality is alkalescence.
Said healthy trace elements with household content is following: zinc: 0.0325-16.4 μ g/L; Selenium: 0.0395-0.712 μ g/L; Chromium: 0.104-4.54 μ g/L; Manganese 0.156-5.52 μ g/L.
Said poisonous micronutrient levels is following: lead＜5 μ g/L; Hydrargyrum＜0.5 μ g/L; Arsenic＜5 μ g/L; Cadmium＜1 μ g/L.
Use pH meter that its pH value is measured (25 ℃), the result shows healht-care liquid pH value in 7.5 ± 0.3 scopes, and water quality is alkalescence.
Two, the pharmacodynamic experiment-cellular level of healht-care liquid prophylaxis of hyperlipidemia
1, experimental technique
The take the logarithm HepG2 cell of trophophase is after trypsinization 5 * 10
5Cell/mL is inoculated in 6 orifice plates; Treat that cell length degree of converging is about 70%, be changed to the DMEM that does not contain serum, hungry 12h; Add respectively different hardness healht-care liquid (hardness is respectively 200,400,600,800,1000ppm), the lovastatin of 1 μ mol/L (Lovastatin, Lova) as positive control, blank control group gives distilled water; Behind the 24h, absorb remaining culture liq, ice PBS washes 2 times, adds 100 μ L cell pyrolysis liquids, and piping and druming was evenly incubated in the frozen water 30 minutes, during constantly scrape and smear with cell, lysate is fully contacted with cell; Lysate is transferred in the 1.5mLep pipe low temperature ultrasonication 10s; 4 ℃ of 12000rpm 15min are centrifugal, and-80 ℃ of freezing preservations are used to detect intracellular lipid content; Protein content determination:, press the operation of test kit description with BCA determination of protein concentration kit measurement; Measure the content of interior T-CHOL (TC) of cell and triglyceride (TG) respectively: TC content is pressed the cholesterol determination test kit
The description of Red Cholesterol Assay Kit is measured; TG content is pressed Infinity
TMTriglycerides Reagent description is carried out; Unit calculates with lipid content in every milligram of protein (μ g/mg protein).
2, experimental result
Can find out by table 1; Through hatching of 24h; This healht-care liquid can reduce HepG2 born of the same parents' inner lipid content in dose dependent ground; Wherein hardness is 800 can significantly reduce the content of the TC in the HepG2 born of the same parents with the healht-care liquid of 1000ppm, and hardness is the content that the healht-care liquid of 1000ppm can significantly reduce TG in the born of the same parents.
Table 1: healht-care liquid is to the influence of HepG2 cell born of the same parents inner lipid content
Annotate: contrast with blank control group:
#P＜0.05,
##P＜0.01;
Three, the pharmacodynamic experiment of health-care drinking-water prophylaxis of hyperlipidemia-animal level
1, experimental technique
40 of Wistar rats, male, body weight 180-220g is divided into blank control group, model group, positive control (Lova) group and healht-care liquid group, 10 every group at random.Experimental session rat ad lib normal diet and drinking-water, blank control group, model group, Lova group all give distilled water, and the health-care drinking-water group gives the healht-care liquid that hardness is 1000ppm.Every morning Lova group is irritated stomach lovastatin (5mgkg-1), and all the other each groups are irritated stomach 5 ‰ CMC-Na; Afternoon, blank control group was irritated stomach equal-volume soil temperature-80 and propylene glycol mixture (volume fraction 1: 1); All the other each groups are irritated the high lipoprotein emulsion of stomach (25% Adeps Sus domestica, 10% cholesterol, 2% sodium cholate, 1% propylthiouracil; 20% Tween 80,20% propylene glycol and 22% water), irritate stomach dosage and be 10mLkg-1.Observe the rat growing state every day, comprise behavior, hair etc., accurate recording food ration, water uptake; Set time weighs in weekly.Be 28d experimental period.After the last administration, water 12h is can't help in the rat fasting, plucks eyeball and gets blood; Centrifuging and taking serum is measured T-CHOL (TC) in the serum, triglyceride (TG); Low-density lipoprotein cholesterol (LDL-C), HDL-C (HDL-C), malonaldehyde (MDA); Superoxide dismutase (SOD), glutathion peroxidase (GSH-Px), glutamic oxaloacetic transaminase, GOT (AST) and glutamate pyruvate transaminase (ALT) content; Peel off liver, kidney, weigh, calculate organ index; The preparation LH, centrifugal, get supernatant and measure liver TC and TG; Animal livers is fixed with fixative, cuts into slices behind the FFPE, and histopathological examination is carried out in HE dyeing.
2, experimental result
1) healht-care liquid is to the influence of hyperlipidemia rats body weight, food ration and amount of drinking water: experimental session blank control group rat in order, hair color is glossy, activity freely, it is normal to ingest, drink water; It is good that the model group rat begins feed, and along with the prolongation of modeling time, the rat hair color is gradually yellow partially, and low in glossiness is rolled up less and moved, and loses weight, and the feed water inlet reduces; And give after the healht-care liquid, the behavior of rat, hair etc. all make moderate progress, and body weight, food ration and amount of drinking water all have the normal trend of recovery.
Table 2: healht-care liquid to the influence of hyperlipidemia rats body weight (
n=10, g)
Annotate: contrast with blank control group:
#P＜0.05,
##P＜0.01; Contrast with model group:
*P＜0.05,
*P＜0.01
Table 3: healht-care liquid to the influence of hyperlipidemia rats food ration (
n=10, g/day)
Annotate: contrast with blank control group:
#P＜0.05,
##P＜0.01; Contrast with model group:
*P＜0.05,
*P＜0.01
Table 4: healht-care liquid to the influence of hyperlipidemia rats amount of drinking water (
n=10, mL/day)
Annotate: contrast with blank control group:
#P＜0.05,
##P＜0.01; Contrast with model group:
*P＜0.05,
*P＜0.01
2) healht-care liquid is to the influence of hyperlipidemia rats blood lipid level: can learn that from table 5 high lipoprotein emulsion can significantly improve rat model serum TC, TG, LDL-C content, and the atherogenic index AI (LDL-C/HDL-C) that significantly raises, the modeling success is described.Give the healht-care fluid power and significantly reduce rat model serum TC, LDL-C content, the healht-care fluid power reduces rat model serum TG content, the trend normal level.Explain that healht-care liquid can improve rat model blood fat disorder state.
Table 5: health-care drinking-water to the influence of hyperlipidemia rats blood lipid level (x ± s, n=10)
Annotate: contrast with blank control group:
#P＜0.05,
##P＜0.01; Contrast with model group:
*P＜0.05,
*P＜0.01
3) healht-care liquid is the important antioxidase of body to hyperlipidemia rats Content of MDA, SOD and GSH-PX active influence: SOD, GSH-Px, and is sensitive to the IR of peroxidating material.MDA then is the end-product of lipid peroxidation, is the sensitive index of reflection body level of lipid.Visible by table 6, to compare with the blank control group rat, Content of MDA significantly improves in the model group rat body, and SOD and GSH-PX are active significantly to be reduced, and is in the peroxidating state.After giving healht-care liquid 28d, can significantly reduce MDA content in the serum, it is active to improve serum GSH-PX, significantly improves rat blood serum SOD enzymatic activity.Show that the healht-care fluid power significantly improves the peroxidating state of hyperlipidemia rats body.
Table 6: healht-care liquid is to the influence (
n=10) of hyperlipidemia rats SOD in serum, GSH-Px, MDA
Annotate: contrast with blank control group:
#P＜0.05,
##P＜0.01; Contrast with model group:
*P＜0.05,
*P＜0.01
4) healht-care liquid is to the influence of hyperlipidemia rats serum lipoprotein metabolic enzyme activity: can be found out that by table 7 with compared with normal, model group LPL, HL, the active all significances of LCAT reduce; Healht-care liquid then can significantly improve the activity of lipoprotein metabolism enzymes such as LPL, HL, LCAT.
Table 7: healht-care liquid to the influence of hyperlipidemia rats serum serum lipoprotein metabolic enzyme activity (
n=10, Kat/L)
Annotate: contrast with blank control group:
#P＜0.05,
##P＜0.01; Contrast with model group:
*P＜0.05,
*P＜0.01
5) healht-care liquid is to the influence of hyperlipidemia rats organ coefficient: fat metabolic disturbance possibly cause hepatocyte that lipoid degeneration takes place, and causes the enlargement degeneration of liver then; And hyperlipidemia possibly increase the renal metabolism burden, makes it that atrophy take place.As shown in table 8, irritate to feed that tangible fat metabolic disturbance has appearred in the hyperlipidemia mice due to the lipomul, to compare with blank control group, model group rats'liver coefficient increases, the kidney coefficient reduces, and shows that liver, kidney have damage.After giving healht-care liquid, the lipid metabolism of rat gradually in normally, has reduced the Liver and kidney burden, and hepatic and renal function is improved, and the Liver and kidney index all trends towards normally.
Table 8: healht-care liquid is to hyperlipidemia rats organ index influence (
n=10)
Annotate: contrast with blank control group:
#P＜0.05,
##P＜0.01; Contrast with model group:
*P＜0.05,
*P＜0.01
6) healht-care liquid is to the influence of hyperlipidemia rats liver TC and TG: as shown in table 9, with compared with normal, TC, TG significantly raise in the rat model liver, explain that high lipoprotein emulsion seriously upset the lipid metabolism of rat liver.The healht-care fluid power significantly reduces rat model liver TC, TG level, regulates the rat liver lipid metabolism.
Table 9: healht-care liquid is to the influence (
n=10) of hyperlipidemia rats liver TC and TG
Annotate: contrast with blank control group:
#P＜0.05,
##P＜0.01; Contrast with model group:
*P＜0.05,
*P＜0.01
7) healht-care liquid is to the influence of hyperlipidemia rats serum AST and ALT: visible with respect to blank control group by table 10; AST and the ALT of model group rat significantly raise (p＜0.01); Explain that the model group hepatocyte is seriously downright bad; High lipoprotein emulsion has caused major injury to the liver of model group rat, and healht-care liquid group then can significantly suppress the caused ALT of high lipoprotein emulsion, AST raises, and repairs the hepatic tissue degeneration.
Table 10: healht-care liquid is to the influence (
n=10) of hyperlipidemia rats serum alt, AST
Annotate: contrast with blank control group:
#P＜0.05,
##P＜0.01; Contrast with model group:
*P＜0.05,
*P＜0.01
8) liver organization pathological observation result: each organizes the rat liver perusal: the blank control group rat liver is kermesinus, and quality is even, form rule, no abnormality seen; The yellowing of model group rat liver color and luster, HUANGBAI(sic) is alternate, and that touches has a greasy feeling.Pathological examination shows that blank control group rats'liver leaflet structure is clear complete, liver rope queueing discipline, and hepatocyte form rule is arranged normal; Model group lobules of liver structural fuzzy is unclear, occurs fat vacuole not of uniform size in the hepatocyte arrangement disorder, hepatocyte endochylema, and the hepatocyte enlargement is obvious.Healht-care liquid group hepatic tissue structure is roughly normal, and still there is a certain amount of fat vacuole in part of hepatocytes rope arrangement disorder, but the liver fat degeneration obviously alleviates.
9) healht-care liquid is to the influence of hyperlipidemia rats fasting glucose content: hyperlipemia is a kind of chronic metabolic disease, and generation, the development of itself and diabetes have substantial connection.Table 11 can find out that the fasting glucose content of model group obviously raises than normal group, gives the rising that healht-care liquid then can significantly suppress fasting glucose.This our healht-care liquid of prompting might have the excellent prevention effect for the concurrent diabetes of hyperlipemia.
Table 11: healht-care liquid is to the influence (
n=10) of hyperlipidemia rats blood sugar content
Annotate: contrast with blank control group:
#P＜0.05,
##P＜0.01; Contrast with model group:
*P＜0.05,
*P＜0.01
This experimental result shows; This healht-care fluid power enough significantly reduces the lipid content in the HepG2 cell born of the same parents; And regulate hyperlipidemia rats blood fat disorder state effectively from many aspects; Blood lipid reducing level and AI (LDL-C/HDL-C) reduce the liver accumulation of lipid simultaneously, and significantly reduce fasting glucose content; The prompting health-care drinking-water can significantly be regulated body lipid metabolic disorder and concurrent fatty liver and diabetes thereof, and metabolic diseases such as hyperlipemia are had preventive effect preferably.
The healht-care liquid that hyperlipidemia patient can be selected corresponding hardness according to oneself health and dietary habit; If diet is less to the picked-up of mineral; Can select the healht-care liquid (600-1000ppm) of high rigidity for use, otherwise select healht-care liquid (200-400ppm) than soft.
This healht-care liquid derives from deep seawater, has aboundresources, is easy to industrialization, and safe advantages of higher, aspect the preventing and treating of hyperlipemia, has vast market and application prospect.The present invention will be the higher value application of deep seawater offer reference meaning and enlightenment.
Above embodiment is only in order to explaining technical scheme of the present invention, but not limits it; Although the present invention has been carried out detailed explanation with reference to previous embodiment, for the person of ordinary skill of the art, still can make amendment to the technical scheme that previous embodiment is put down in writing, perhaps part technical characterictic wherein is equal to replacement; And these modifications or replacement do not make the essence of relevant art scheme break away from the spirit and the scope of the present invention's technical scheme required for protection.
Claims (9)
1. a healht-care liquid that contains deep seawater is in preparation prevention or the medicine of treatment hyperlipemia or the application in the health product; Said healht-care liquid comprises A liquid and B liquid, and said A liquid is fresh water, and said B liquid makes through following steps: deep seawater is isolated dense water section behind reverse osmosis unit; Dense water section is carried out concentrating under reduced pressure; Make its volume be concentrated into the 0.67-3.33% of original volume, remove by filter the sodium chloride of separating out then, the height concentrated solution that obtains is B liquid; The hardness of said healht-care liquid is at 200-1000 ppm, and said deep seawater is below the 500m of sea level and the sea water after desalting processing.
2. a kind of healht-care liquid that contains deep seawater according to claim 1 is in preparation prevention or the medicine of treatment hyperlipemia or the application in the health product; It is characterized in that magnesium in the said healht-care liquid: calcium: potassium: the concentration ratio of sodium element is 2.5-3.5:0.45-1:0.3-1:0.3-1.5; Do not contain Organic substance, and water quality is alkalescence.
3. a kind of healht-care liquid that contains deep seawater according to claim 2 is characterized in that said magnesium: calcium in preparation prevention or the medicine of treatment hyperlipemia or the application in the health product: the ratio of greater inequality of the concentration of potassium is 3:1:1.
4. a kind of healht-care liquid that contains deep seawater according to claim 2 is in preparation prevention or the medicine of treatment hyperlipemia or the application in the health product; The water quality that it is characterized in that said healht-care liquid is alkalescence, the pH value 7.5 ± 0.3 of 25 ℃ of mensuration.
5. a kind of healht-care liquid that contains deep seawater according to claim 1 is characterized in that the beneficial element content in the said healht-care liquid is: zinc 0.0325-16.4 μ g/L in preparation prevention or the medicine of treatment hyperlipemia or the application in the health product; Selenium 0.0395-0.712 μ g/L; Chromium 0.104-4.54 μ g/L; Manganese 0.156-5.52 μ g/L.
6. a kind of healht-care liquid that contains deep seawater according to claim 1 is characterized in that the harmful element content in the said healht-care liquid is: plumbous < 5 μ g/>L in preparation prevention or the medicine of treatment hyperlipemia or the application in the health product; Hydrargyrum < 0. 5 μ g/>L; Arsenic < 5 μ g/>L; Cadmium < 1 μ g/>L.
7. a kind of healht-care liquid that contains deep seawater according to claim 1 is characterized in that in preparation prevention or the medicine of treatment hyperlipemia or the application in the health product said deep seawater is taken from apart from beyond the 20km of coastline.
8. a kind of healht-care liquid that contains deep seawater according to claim 1 is characterized in that said fresh water sources Yu Haiyang deep water isolated fresh water after the reverse osmosis unit circulation in preparation prevention or the medicine of treatment hyperlipemia or the application in the health product.
9. a kind of healht-care liquid that contains deep seawater according to claim 1 is in preparation prevention or the medicine of treatment hyperlipemia or the application in the health product; It is characterized in that it is the healht-care liquid of 600-1000 ppm that diet is selected hardness for use to the less hyperlipidemia patient of the picked-up of mineral, is the healht-care liquid of 200-400 ppm otherwise select hardness.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210114603.5A CN102652758B (en) | 2012-04-18 | 2012-04-18 | Application of health care drinking liquid containing deep seawater in preparation of medicaments or health care products for preventing or treating hyperlipidemia |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210114603.5A CN102652758B (en) | 2012-04-18 | 2012-04-18 | Application of health care drinking liquid containing deep seawater in preparation of medicaments or health care products for preventing or treating hyperlipidemia |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102652758A true CN102652758A (en) | 2012-09-05 |
| CN102652758B CN102652758B (en) | 2014-02-26 |
Family
ID=46728590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210114603.5A Active CN102652758B (en) | 2012-04-18 | 2012-04-18 | Application of health care drinking liquid containing deep seawater in preparation of medicaments or health care products for preventing or treating hyperlipidemia |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102652758B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106211749A (en) * | 2014-03-31 | 2016-12-07 | 株式会社阿丽浱欧 | Comprise the functional drinks of the mineral water of the high rigidity made from salty subsoil water or deep seawater |
| CN111184737A (en) * | 2020-02-28 | 2020-05-22 | 山东中医药大学 | Application of fucoidan and deep seawater in prevention and treatment of diabetes cognitive impairment |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101574166A (en) * | 2009-06-05 | 2009-11-11 | 杨健 | Ocean deep water-containing drinking water with health care function |
-
2012
- 2012-04-18 CN CN201210114603.5A patent/CN102652758B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101574166A (en) * | 2009-06-05 | 2009-11-11 | 杨健 | Ocean deep water-containing drinking water with health care function |
Non-Patent Citations (1)
| Title |
|---|
| 吴小红: "深海水的药理活性：对高脂血症的防治作用", 《国外医学中医中药分册》, vol. 27, no. 1, 31 December 2005 (2005-12-31) * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106211749A (en) * | 2014-03-31 | 2016-12-07 | 株式会社阿丽浱欧 | Comprise the functional drinks of the mineral water of the high rigidity made from salty subsoil water or deep seawater |
| CN111184737A (en) * | 2020-02-28 | 2020-05-22 | 山东中医药大学 | Application of fucoidan and deep seawater in prevention and treatment of diabetes cognitive impairment |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102652758B (en) | 2014-02-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102250984B (en) | Method for preparing functional extracellular polysaccharide of lactic acid bacteria | |
| CN109939027A (en) | A kind of method of cosmetics stoste of the Hericium erinaceus fermentation preparation containing erythrothioneine | |
| CN101683351B (en) | Application of phlorizin in preparing hepatic or health-care food | |
| EA027917B1 (en) | Algal lipid compositions and methods of preparing and utilizing the same | |
| CN110846346A (en) | Microbial oil rich in Sn-2 DHA, and preparation method and application thereof | |
| CN102504039A (en) | Extraction process of black corn silk polysaccharide | |
| CN102652757B (en) | Application of health-care drinking liquid containing deep seawater in preparation of medicaments or health-care products for preventing or treating metabolic syndrome | |
| CN102406209A (en) | Liquid drink containing active ingredients of rose essential oil and preparation method for liquid drink | |
| CN104045121A (en) | Preparation and application methods of micromolecular water | |
| CN102652758B (en) | Application of health care drinking liquid containing deep seawater in preparation of medicaments or health care products for preventing or treating hyperlipidemia | |
| CN103627640A (en) | Low-chlorine medium used for culturing Schizochytrium limacinum and production method for docosahexenoic acid (DHA) by using same | |
| CN102429928A (en) | Preparation method for coix seed red yeast rice | |
| CN103773690B (en) | A kind of method of open cultivation microalgae | |
| CN102485237A (en) | Sweet wormwood herb brewed liquid, brewing technology thereof, and preparation method of starter thereof | |
| CN105053567A (en) | Method for producing yeast-fermented copper feed additive by beer wastewater | |
| CN104629981A (en) | Honey fermented wine and preparation method thereof | |
| CN101450089A (en) | Mangrove extract liquid and preparation method thereof | |
| CN102697807B (en) | Application of health-care drinking liquid in preparation of medicines or health-care products for preventing or treating II-type diabetes mellitus, diabetes mellitus and diabetic nephropathy | |
| CN103103127A (en) | Culture method for microalgae | |
| CN102311293A (en) | Preparation method for compound microorganism liquid state fertilizer | |
| CN101244084A (en) | Sobering-up agent of pearl oyster meat and preparation thereof | |
| CN101890073A (en) | Chinese medicinal composition for preventing and treating hyperlipaemia and fatty liver and preparation method thereof | |
| CN103933089A (en) | Selenium enriched dahurian angelica root decoction piece prepared by fermentation and transformation of a plurality of microbial bacterium groups and preparation method thereof | |
| CN103499547A (en) | Method for determination of L-carnitine by enzymatic spectrophotometry | |
| CN104544444B (en) | A kind of Caulis et Folium Tritici aestivi health beverage and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |