CN102652758B - Application of health care drinking liquid containing deep seawater in preparation of medicaments or health care products for preventing or treating hyperlipidemia - Google Patents
Application of health care drinking liquid containing deep seawater in preparation of medicaments or health care products for preventing or treating hyperlipidemia Download PDFInfo
- Publication number
- CN102652758B CN102652758B CN201210114603.5A CN201210114603A CN102652758B CN 102652758 B CN102652758 B CN 102652758B CN 201210114603 A CN201210114603 A CN 201210114603A CN 102652758 B CN102652758 B CN 102652758B
- Authority
- CN
- China
- Prior art keywords
- healht
- liquid
- care liquid
- deep seawater
- health care
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention provides an application of health care drinking liquid containing deep seawater in the preparation of medicaments or health care products for preventing or treating hyperlipoidemia. The health care drinking liquid comprises A liquid and B liquid, wherein the A liquid is of fresh water, the B liquid is of high-concentration concentrated liquid which is obtained by performing decompression concentration on a concentrated water part which is separated from the deep sweater via a reverse osmosis device, and the hardness range is 200-1000ppm. The health care drinking liquid is given to a rat with the hyperlipoidemia, which is subjected to modeling by a high-fat emulsion, in a free drinking way, after the medicament is administrated for 28 days, the blood lipid level of the rat with the hyperlipoidemia can be significantly reduced, lipid peroxidation can be inhibited, the activity of lipoprotein metabolic enzymes, namely LCAT (lecithin-cholesterol acetyltransferase), LPL (lipoprotein lipase) and HL (hepatic lipase), can be significantly improved, and the state of lipid metabolism disorder in the rat with the hyperlipoidemia, as well as concurrent fatty liver and diabetes thereof, can be further improved. The health care drinking liquid is derived from the deep seawater, has the advantages of abundant resources, easiness in industrialization and the like, and further has broad market and application prospects in the aspect of preventing and treating the hyperlipoidemia.
Description
Technical field
The invention belongs to marine biotechnology field, relate to the comprehensive utilization of deep seawater, be specifically related to a kind of healht-care liquid that contains deep seawater in preparation prevention or the medicine for the treatment of hyperlipemia or the application in health product.
Background technology
Along with improving constantly of people's living standard, all there is great variation in life style and dietary structure, and the sickness rate of the cardiovascular and cerebrovascular diseases such as hyperlipemia is also more and more higher.Hyperlipemia is called again lipid metabolic disorder or abnormal, mainly refers to that TC, LDL-C and the TG in blood is too low higher than normal range and/or HDL-C level.Lipid metabolic disorder can cause that blood viscosity increases, slow blood flow, and lipidosis too much in blood, in Wall of Artery, forms atherosclerotic plaque, causes stricture of artery, and blood flow is obstructed, and target organ blood supply reduces and then forms damage.Brain arteriosclerosis can cause cerebral hemorrhage or cerebral thrombosis; Heart coronary artery sclerosis can cause uncomfortable in chest, nervous, and severe patient can cause myocardial infarction.In addition, hyperlipemia is also an important risk factor that promotes hypertension, impaired glucose tolerance, diabetes.Research is found in the pathogeny of cardiovascular and cerebrovascular disease, hyperlipemia is a main independent hazard factor, serum lipids level declines 1%, the sickness rate of coronary heart disease will reduce by 2%, so control generation and the development of hyperlipemia, for prevention cardiovascular and cerebrovascular disease, have great significance, the prevention of hyperlipemia and treatment have become current study hotspot.
In the treatment of hyperlipemia, statins is most widely used general, can effectively reduce the concentration of LDL-C in blood, can reduce incidence rate and the coronary heart disease death rate of Cardiovascular events after life-time service.But this medicine has certain side effect, such as liver function injury, rhabdomyolysis, myalgia, weak, erythra etc.; And after drug withdrawal, blood fat gos up comparatively fast, is difficult to reach the object of long-term treatment and prevention.
Adjust the basis that dietary habit and the mode of making the life better are the various hyperlipemias for the treatment of, especially for idiopathic hyperlipidemia patient, should first-selected Diet Therapy.The people's such as Yang Weiguo research shows: along with the change of diet structure, 75% blood lipid has decline in various degree.The decline of U.S.'s crowd's blood lipid level over nearly 20 years is relevant to the attention degree of Diet Therapy with doctor and patient.Therefore, research and development have the food of effect for reducing fat, thereby significant to improving hyperlipidemia by adjustment diet structure.
Research shows, the generation of the cardiovascular and cerebrovascular disease such as mineral element and hyperlipemia and develop closely related, by diet, supplement useful mineral element magnesium, calcium, chromium, manganese, zinc, selenium, reduce the contact of Harmful Trace Elements hydrargyrum, lead etc. simultaneously, to maintain the relatively stable of Mineral Concentrations in body, the positive effect that has to cardiovascular and cerebrovascular diseases such as prevention hyperlipemias.
Deep seawater typically refer to solar radiation less than, can not carry out photosynthesis, the sea water below the about 200m degree of depth.The ocean of deep seawater in entering without sunlight be without photosphere, and away from impact and the pollution of the chemical substance from the mankind, land and atmosphere.Research shows, deep seawater has following main feature: (1) low temperature homeostasis-be not subject to solar radiation, and changeable unlike sea surface coolant-temperature gage, deep seawater is temperature-resistant all the year round, is constant at 5 ℃ of left and right; (2) the abundant and stability of mineral element-with sea surface water ratio, deep seawater is rich in 92 kinds of mineral elements such as the necessary calcium of human body, magnesium, potassium, sodium, ferrum, copper, lithium, molybdenum, manganese, zinc, germanium, iodine, phosphorus, fluorine, selenium, trivalent chromium, silicate ion, has contained the required element kind of human body metabolism.Except its rich content, because these water flow in the bathypelagic of " without photosphere " with the very long time, without photosynthesis, ectocine occurs, is not subject to, therefore the composition of contained mineral element is very stable, has ideally solved the requirement of the various required mineral elements of total balance of the body picked-up; (3) deep water of aseptic spatter property-in ocean " without photosphere ", except the impact away from mankind's modern civilization and be not subject to the pollution of land, Atmospheric Chemistry material, pathogenic bacteria, itself is also without the condition that generates the antibacterials such as pathogen.Therefore, deep seawater is the water of the aseptic nature that cleans very much; (4) deep seawater water quality is alkalescence.
Owing to having above feature, deep seawater is being subject to attracting attention of common people as a kind of new natural resources.Deep seawater is rich in various mineral and trace element, and it has good improvement effect to hyperlipemia in theory.China marine site is broad, and coastline is very long, and marine resources are abundant, but there is no the research of deep seawater Application and Development aspect; And China's water scarcity, water is seriously polluted, and the bathypelagic water resource of exploitation China has very important meaning.
Summary of the invention
The hot issues such as, water pollution day by day in short supply for current China water resource is on the rise and hyperlipemia sickness rate sharply raises, the invention provides a kind of healht-care liquid that contains deep seawater in preparation prevention or the medicine for the treatment of hyperlipemia or the application in health product.
For achieving the above object, the present invention adopts following technical proposals to be achieved:
A kind of healht-care liquid that contains deep seawater is in preparation prevention or the medicine for the treatment of hyperlipemia or the application in health product, described healht-care liquid comprises A liquid and B liquid, described A liquid is fresh water, described B liquid makes through following steps: deep seawater is isolated dense water section after reverse osmosis unit, dense water section is carried out to concentrating under reduced pressure, make its volume be concentrated into the 0.67-3.33% of original volume, then remove by filter the sodium chloride of separating out, the height concentrated solution obtaining is B liquid, the hardness of described healht-care liquid is at 200-1000ppm, described deep seawater is below the 500m of sea level and the sea water after desalting processing.
Further improvement to technique scheme: magnesium in described healht-care liquid: calcium: potassium: the concentration ratio of sodium element is 2.5-3.5: 0.45-1: 0.3-1: 0.3-1.5, not containing Organic substance, and water quality is alkalescence.
Further improvement to technique scheme: described magnesium: calcium: the ratio of greater inequality of concentration of potassium is 3: 1: 1.
Further improvement to technique scheme: the water quality of described healht-care liquid is alkalescence, the pH value 7.5 ± 0.3 of 25 ℃ of mensuration.
Further improvement to technique scheme: the beneficial element content in described healht-care liquid is: zinc 0.0325-16.4 μ g/L; Selenium 0.0395-0.712 μ g/L; Chromium 0.104-4.54 μ g/L; Manganese 0.156-5.52 μ g/L.
Further improvement to technique scheme: the harmful element content in described healht-care liquid is: plumbous < 5 μ g/L; Hydrargyrum < 0.5 μ g/L; Arsenic < 5 μ g/L; Cadmium < 1 μ g/L.
Further improvement to technique scheme: described deep seawater is taken from apart from beyond the 20km of coastline.
Further improvement to technique scheme: described fresh water sources Yu Haiyang deep water is isolated fresh water after reverse osmosis unit circulation.
Further improvement to technique scheme: it is the healht-care liquid of 600-1000ppm that diet is selected hardness to the less hyperlipidemia patient of the picked-up of mineral, on the contrary the healht-care liquid that hardness is 200-400ppm selected.
Compared with prior art, advantage of the present invention and good effect are:
The healht-care liquid that contains deep seawater provided by the invention is rich in healthy trace elements with household magnesium, calcium, vanadium, chromium, manganese, zinc, selenium, and Harmful Trace Elements hydrargyrum and plumbous content are very low, this healht-care liquid can significantly reduce serum lipids level and atherogenic index (LDL-C/HDL-C), reduce liver accumulation of lipid simultaneously, and significantly reduce fasting glucose content, point out this healht-care liquid can significantly regulate body lipid metabolic disorder and concurrent fatty liver and diabetes thereof, the metabolic diseases such as hyperlipemia are had to good preventive and therapeutic action.
The specific embodiment
Below in conjunction with the specific embodiment, technical scheme of the present invention is described in further detail.
Embodiment 1
One, the preparation of healht-care liquid
The preparation method of healht-care liquid of the present invention is as follows:
1) bathypelagic water pretreatment: will take from apart from beyond the 20km of coastline, sea level 500m puts into water tank with dark deep seawater, adopts filter to filter, and removes phytoplankton and microorganism in sea water, and filter by active carbon filter, further remove Organic substance.
2) fresh water is separated with dense water: the deep seawater obtaining after pretreatment is entered to reverse osmosis unit by high-pressure pump and deep seawater is divided into fresh water part (fresh water part water quality is identical with pure water, or water quality approaches pure water with dense water section; In dense water section, the concentration of various zwitterions approaches 2 times of various ion concentrations in former sea water), isolated fresh water is partly that A liquid enters fresh water water tank, isolated dense water section enters dense water water tank.The technological parameter of reverse osmosis unit is: 4MPa, power 1.5KW, aquifer yield 1T/d are pressed in running.
3) the de-sodium of dense water is processed: the dense water section in dense water water tank is carried out to concentrating under reduced pressure, make its volume be concentrated into the 0.67-3.33% of original volume, then remove by filter the sodium chloride of separating out, the concentrated solution that obtains highly concentrating is B liquid, magnesium in described concentrated solution: calcium: potassium: the concentration ratio of sodium element is 2.5-3.5: 0.45-1: 0.3-1: 0.3-1.5, described magnesium, calcium, potassium, the content concn unit of sodium element in concentrated solution are mg/L.Trace element chromium wherein, zinc, selenium, the concentration of manganese etc. and cycles of concentration are multiple proportions relation.
Or, also can adopt selectivity electrodialysis to reverse osmosis after isolated dense water section take off sodium and process, electrodialysis plant adopts 1 valency cation selective to see through film, cavity block adopts conventional ion exchange membrane.In electrodialytic process, all aniones all can see through cavity block, and only univalent cation sees through anode membrane, and the polyvalent cation in the dense water after de-sodium is close with the concentration in former dense water.
4) healht-care liquid fill: by through step 1) reverse osmosis unit fresh water A liquid and step 3 out) resulting B liquid can mix the healht-care liquid that make different hardness according to demand, and described hardness is with CaCO
3cubage (GB 5749-2006 drinking water sanitary standard), its computing formula is: hardness=Ca (ppm)) * 2.5+Mg (ppm) * 4.1, through high temperature sterilize, packing.
Adopt inductively coupled plasma mass spectrum (ICP-MS) to measure the inorganic element content in healht-care liquid: the healht-care liquid that the hardness of take is 1000 is example, and wherein several main mine materials and micronutrient levels are: magnesium: 200-224mg/L; Calcium: 33-67mg/L; Potassium: 20-67mg/L; Sodium: 20-100mg/L (content of sodium is more low better); Zinc: 0.0325-16.4 μ g/L; Selenium: 0.0395-0.712 μ g/L; Chromium: 0.104-4.54 μ g/L; Manganese 0.156-5.52 μ g/L, plumbous < 5 μ g/L; Hydrargyrum < 0.5 μ g/L; Arsenic < 5 μ g/L; Cadmium < 1 μ g/L.
Healht-care liquid mineral under 200-1000 hardness of the present invention and micronutrient levels are directly proportional to its hardness.Magnesium in described healht-care liquid: calcium: potassium: the concentration ratio of sodium element is 2.5-3.5: 0.45-1: 0.3-1: 0.3-1.5, is rich in healthy trace elements with household zinc, selenium, chromium, manganese, Harmful Trace Elements is plumbous, hydrargyrum, arsenic, cadmium content is lower, and not containing Organic substance, and water quality is alkalescence.
Described healthy trace elements with household content is as follows: zinc: 0.0325-16.4 μ g/L; Selenium: 0.0395-0.712 μ g/L; Chromium: 0.104-4.54 μ g/L; Manganese 0.156-5.52 μ g/L.
Described Harmful Trace Elements content is as follows: plumbous < 5 μ g/L; Hydrargyrum < 0.5 μ g/L; Arsenic < 5 μ g/L; Cadmium < 1 μ g/L.
Use pH meter to measure (25 ℃) to its pH value, result shows that healht-care liquid pH value is in 7.5 ± 0.3 scopes, and water quality is alkalescence.
Two, the pharmacodynamic experiment-cellular level of healht-care liquid prevention hyperlipemia
1, experimental technique
The take the logarithm HepG2 cell of trophophase, after trypsinization 5 * 10
5cell/mL is inoculated in 6 orifice plates; Treat cell length degree of converging approximately 70%, be changed to the DMEM that does not contain serum, hungry 12h; (hardness is respectively 200,400,600,800, and 1000ppm), the lovastatin (Lovastatin, Lova) of 1 μ mol/L is as positive control, and blank group gives distilled water to add respectively the healht-care liquid of different hardness; After 24h, absorb remaining culture liq, ice PBS washes 2 times, adds 100 μ L cell pyrolysis liquids, and piping and druming evenly, is incubated in frozen water 30 minutes, during constantly with cell, scrape and smear, lysate is fully contacted with cell; Lysate is transferred in 1.5mLep pipe to low temperature ultrasonication 10s; 4 ℃ of 12000rpm 15min are centrifugal, and-80 ℃ of freezing preservations, for detection of intracellular lipid content; Protein content determination: with BCA determination of protein concentration kit measurement, operate by test kit description; Measure respectively the content of the interior T-CHOL (TC) of cell and triglyceride (TG): TC content is by cholesterol determination test kit
the description of Red Cholesterol Assay Kit is measured; TG content is pressed Infinity
tMtriglycerides Reagent description is carried out; Unit calculates with lipid content in every milligram of protein (μ g/mg protein).
2, experimental result
As can be seen from Table 1, through hatching of 24h, this healht-care liquid can reduce HepG2 born of the same parents' inner lipid content in dose dependent ground, wherein hardness be 800 and the healht-care liquid of 1000ppm can significantly reduce the content of the TC in HepG2 born of the same parents, the healht-care liquid that hardness is 1000ppm can significantly reduce the content of TG in born of the same parents.
Table 1: the impact of healht-care liquid on HepG2 cell born of the same parents inner lipid content
Note: contrast with blank group:
#p < 0.05,
##p < 0.01;
Three, pharmacodynamic experiment-animal level of health-care drinking-water prevention hyperlipemia
1, experimental technique
40 of Wistar rats, male, body weight 180-220g, is divided into blank group, model group, positive control (Lova) group and healht-care liquid group, 10 every group at random.Experimental session rat ad lib normal diet and drinking-water, blank group, model group, Lova group all give distilled water, and health-care drinking-water group gives the healht-care liquid that hardness is 1000ppm.Every morning Lova organizes gavage lovastatin (5mgkg-1), and all the other respectively organize gavage 5 ‰ CMC-Na; Afternoon, blank was organized gavage equal-volume twen-80 and propylene glycol mixture (volume fraction 1: 1), all the other respectively organize the high lipoprotein emulsion of gavage (25% Adeps Sus domestica, 10% cholesterol, 2% sodium cholate, 1% propylthiouracil, 20% Tween 80,20% propylene glycol and 22% water), gavage dosage is 10mLkg-1.Observe Growth in Rats situation every day, comprise behavior, hair etc., accurate recording food ration, water uptake; Set time weighs in weekly.Be 28d experimental period.After last administration, water 12h is can't help in rat fasting, plucks eyeball and gets blood, centrifuging and taking serum, measure T-CHOL (TC) in serum, triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), HDL-C (HDL-C), malonaldehyde (MDA), superoxide dismutase (SOD), glutathion peroxidase (GSH-Px), glutamic oxaloacetic transaminase, GOT (AST) and glutamate pyruvate transaminase (ALT) content; Peel off liver, kidney, weigh, calculate organ index; Preparation liver homogenate, centrifugal, get supernatant and measure liver TC and TG; Animal livers is fixed with fixative, after paraffin embedding, cuts into slices, and HE dyeing, carries out histopathological examination.
2, experimental result
1) impact of healht-care liquid on hyperlipidemia rats body weight, food ration and amount of drinking water: in order, hair color is glossy for the blank group of experimental session rat, and activity freely, is ingested, drunk water and be normal; It is good that model group rat starts feed, and along with the prolongation of modeling time, rat hair color is gradually partially yellow, and low in glossiness, rolls up less and move, and loses weight, and feed water inlet reduces; And after giving healht-care liquid, the behavior of rat, hair etc. all make moderate progress, and body weight, food ration and amount of drinking water have the normal trend of recovery.
Table 2: healht-care liquid on the impact of hyperlipidemia rats body weight (
n=10, g)
Note: contrast with blank group:
#p < 0.05,
##p < 0.01; Contrast with model group:
*p < 0.05,
*p < 0.01
Table 3: healht-care liquid on the impact of hyperlipidemia rats food ration (
n=10, g/day)
Note: contrast with blank group:
#p < 0.05,
##p < 0.01; Contrast with model group:
*p < 0.05,
*p < 0.01
Table 4: healht-care liquid on the impact of hyperlipidemia rats amount of drinking water (
n=10, mL/day)
Note: contrast with blank group:
#p < 0.05,
##p < 0.01; Contrast with model group:
*p < 0.05,
*p < 0.01
2) impact of healht-care liquid on hyperlipidemia rats blood lipid level: can learn from table 5, high lipoprotein emulsion can significantly improve rat model serum TC, TG, LDL-C content, and the atherogenic index AI (LDL-C/HDL-C) that significantly raises, modeling success is described.Give healht-care liquid and can significantly reduce rat model serum TC, LDL-C content, healht-care liquid can make rat model serum TG content reduce, trend normal level.Illustrate that healht-care liquid can improve rat model blood fat disorder state.
Table 5: health-care drinking-water is on the impact of hyperlipidemia rats blood lipid level (x ± s, n=10)
Note: contrast with blank group:
#p < 0.05,
##p < 0.01; Contrast with model group:
*p < 0.05,
*p < 0.01
3) impact of healht-care liquid on hyperlipidemia rats Content of MDA, SOD and GSH-PX activity: SOD, GSH-Px are the important antioxidase of body, sensitive to the irritant reaction of peroxidating material.MDA is the end-product of lipid peroxidation, is the sensitive indexes of reflection body level of lipid.From table 6, to compare with blank group rat, in model group rat body, Content of MDA significantly improves, and SOD and GSH-PX are active significantly to be reduced, in superoxidant state.Give, after healht-care liquid 28d, can significantly reduce MDA content in serum, improve serum GSH-PX active, significantly improve rat blood serum SOD enzymatic activity.Show that healht-care liquid can significantly improve the superoxidant state of hyperlipidemia rats body.
Table 6: healht-care liquid on the impact of hyperlipidemia rats SOD in serum, GSH-Px, MDA (
n=10)
Note: contrast with blank group:
#p < 0.05,
##p < 0.01; Contrast with model group:
*p < 0.05,
*p < 0.01
4) impact of healht-care liquid on hyperlipidemia rats serum lipoprotein metabolic enzyme activity: as can be seen from Table 7, compare with normal group, model group LPL, HL, the active all significances of LCAT reduce; Healht-care liquid can significantly improve the activity of the lipoprotein metabolism enzymes such as LPL, HL, LCAT.
Table 7: healht-care liquid on the impact of hyperlipidemia rats serum serum lipoprotein metabolic enzyme activity (
n=10, Kat/L)
Note: contrast with blank group:
#p < 0.05,
##p < 0.01; Contrast with model group:
*p < 0.05,
*p < 0.01
5) impact of healht-care liquid on hyperlipidemia rats organ coefficient: fat metabolic disturbance may cause hepatocyte generation lipoid degeneration, causes the enlargement degeneration of liver then; And hyperlipidemia may increase renal metabolism burden, make it that atrophy occur.As shown in table 8, fill with to feed hyperlipidemia mice due to lipomul and occurred obvious fat metabolic disturbance, to compare with blank group, model group rats'liver coefficient increases, kidney coefficient reduces, and shows that liver, kidney have damage.Give after healht-care liquid, the lipid metabolism of rat gradually, in normally, has reduced Liver and kidney burden, and hepatic and renal function is improved, and Liver and kidney index all trends towards normally.
Table 8: healht-care liquid on hyperlipidemia rats organ index impact (
n=10)
Note: contrast with blank group:
#p < 0.05,
##p < 0.01; Contrast with model group:
*p < 0.05,
*p < 0.01
6) impact of healht-care liquid on hyperlipidemia rats liver TC and TG: as shown in table 9, to compare with normal group, in rat model liver, TC, TG significantly raise, and illustrate that high lipoprotein emulsion seriously upset the lipid metabolism of rat liver.Healht-care liquid can significantly reduce rat model liver TC, TG level, regulates Hepaticlipid metabolism.
Table 9: healht-care liquid on the impact of hyperlipidemia rats liver TC and TG (
n=10)
Note: contrast with blank group:
#p < 0.05,
##p < 0.01; Contrast with model group:
*p < 0.05,
*p < 0.01
7) impact of healht-care liquid on hyperlipidemia rats serum AST and ALT: by table 10 as seen with respect to blank group, AST and the ALT of model group rat significantly raise (p < 0.01), illustrate that model group hepatocyte is seriously downright bad, high lipoprotein emulsion has caused major injury to the liver of model group rat, healht-care liquid group can significantly suppress the caused ALT of high lipoprotein emulsion, AST and raise, and repairs hepatic tissue degeneration.
Table 10: healht-care liquid on the impact of hyperlipidemia rats serum alt, AST (
n=10)
Note: contrast with blank group:
#p < 0.05,
##p < 0.01; Contrast with model group:
*p < 0.05,
*p < 0.01
8) pathology of hepar observed result: each organizes rat liver perusal: blank group rat liver is kermesinus, homogeneous, form rule, no abnormality seen; The yellowing of model group rat liver color and luster, HUANGBAI(sic) is alternate, and that touches has a greasy feeling.Pathological examination shows that blank group rats'liver leaflet structure is clear complete, liver rope queueing discipline, and hepatocyte form rule, arranges normal; Model group lobules of liver structural fuzzy is unclear, and fat vacuole not of uniform size appears in hepatocyte arrangement disorder in hepatocyte endochylema, and hepatocyte enlargement is obvious.Healht-care liquid group hepatic tissue structure is roughly normal, and part of hepatocytes rope arrangement disorder, still exists a certain amount of fat vacuole, but liver fat degeneration obviously alleviates.
9) impact of healht-care liquid on hyperlipidemia rats fasting glucose content: hyperlipemia is a kind of chronic metabolic disease, the generation of itself and diabetes, development have substantial connection.Table 11 can find out, the fasting glucose content of model group obviously raises than normal group, gives healht-care liquid and can significantly suppress the rising of fasting glucose.This points out us likely for the concurrent diabetes of hyperlipemia, to have good preventive effect by healht-care liquid.
Table 11: healht-care liquid on the impact of hyperlipidemia rats blood sugar content (
n=10)
Note: contrast with blank group:
#p < 0.05,
##p < 0.01; Contrast with model group:
*p < 0.05,
*p < 0.01
This experimental result shows, this healht-care liquid can significantly reduce the lipid content in HepG2 cell born of the same parents, and effectively regulate hyperlipidemia rats blood fat disorder state from many aspects, reduce serum lipids level and atherogenic index (LDL-C/HDL-C), reduce liver accumulation of lipid simultaneously, and significantly reduce fasting glucose content, prompting health-care drinking-water can significantly regulate body lipid metabolic disorder and concurrent fatty liver and diabetes thereof, and the metabolic diseases such as hyperlipemia are had to good preventive effect.
Hyperlipidemia patient can be selected according to the health of oneself and dietary habit the healht-care liquid of corresponding hardness, if diet is less to the picked-up of mineral, can select the healht-care liquid (600-1000ppm) of high rigidity, otherwise select compared with the healht-care liquid (200-400ppm) of soft.
This healht-care liquid derives from deep seawater, has aboundresources, is easy to industrialization, and safe advantages of higher, aspect the preventing and treating of hyperlipemia, has wide market and application prospect.The present invention will be the higher value application of deep seawater offer reference meaning and enlightenment.
Above embodiment is only in order to technical scheme of the present invention to be described, but not is limited; Although the present invention is had been described in detail with reference to previous embodiment, for the person of ordinary skill of the art, the technical scheme that still can record previous embodiment is modified, or part technical characterictic is wherein equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (4)
1. a healht-care liquid that contains deep seawater is in preparation prevention or the medicine for the treatment of hyperlipemia or the application in health product, described healht-care liquid comprises A liquid and B liquid, described A liquid is fresh water, described B liquid makes through following steps: deep seawater is isolated dense water section after reverse osmosis unit, dense water section is carried out to concentrating under reduced pressure, make its volume be concentrated into the 0.67-3.33% of original volume, then remove by filter the sodium chloride of separating out, the height concentrated solution obtaining is B liquid, the hardness of described healht-care liquid is at 200-1000 ppm, described deep seawater is below the 500m of sea level and the sea water after desalting processing,
Magnesium in described healht-care liquid: calcium: potassium: the concentration ratio of sodium element is 2.5-3.5:0.45-1:0.3-1:0.3-1.5, not containing Organic substance, and water quality is alkalescence;
The water quality of described healht-care liquid is alkalescence, the pH value 7.5 ± 0.3 of 25 ℃ of mensuration;
Beneficial element content in described healht-care liquid is: zinc 0.0325-16.4 μ g/L; Selenium 0.0395-0.712 μ g/L; Chromium 0.104-4.54 μ g/L; Manganese 0.156-5.52 μ g/L;
Harmful element content in described healht-care liquid is: plumbous < 5 μ g/L; Hydrargyrum < 0. 5 μ g/L; Arsenic < 5 μ g/L; Cadmium < 1 μ g/L;
Described deep seawater is taken from apart from beyond the 20km of coastline.
2. a kind of healht-care liquid that contains deep seawater according to claim 1, in preparation prevention or the medicine for the treatment of hyperlipemia or the application in health product, is characterized in that described magnesium: calcium: the ratio of greater inequality of the concentration of potassium is 3:1:1.
3. a kind of healht-care liquid that contains deep seawater according to claim 1, in preparation prevention or the medicine for the treatment of hyperlipemia or the application in health product, is characterized in that described fresh water sources Yu Haiyang deep water isolated fresh water after reverse osmosis unit circulation.
4. a kind of healht-care liquid that contains deep seawater according to claim 1 is in preparation prevention or the medicine for the treatment of hyperlipemia or the application in health product, it is characterized in that it is the healht-care liquid of 600-1000 ppm that diet is selected hardness to the less hyperlipidemia patient of the picked-up of mineral, is the healht-care liquid of 200-400 ppm otherwise select hardness.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210114603.5A CN102652758B (en) | 2012-04-18 | 2012-04-18 | Application of health care drinking liquid containing deep seawater in preparation of medicaments or health care products for preventing or treating hyperlipidemia |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210114603.5A CN102652758B (en) | 2012-04-18 | 2012-04-18 | Application of health care drinking liquid containing deep seawater in preparation of medicaments or health care products for preventing or treating hyperlipidemia |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102652758A CN102652758A (en) | 2012-09-05 |
| CN102652758B true CN102652758B (en) | 2014-02-26 |
Family
ID=46728590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210114603.5A Active CN102652758B (en) | 2012-04-18 | 2012-04-18 | Application of health care drinking liquid containing deep seawater in preparation of medicaments or health care products for preventing or treating hyperlipidemia |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102652758B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20170042198A1 (en) * | 2014-03-31 | 2017-02-16 | Aribio Inc. | Functional beverage including high hardness mineral water produced from salty underground water or deep seawater |
| CN111184737A (en) * | 2020-02-28 | 2020-05-22 | 山东中医药大学 | Application of fucoidan and deep seawater in prevention and treatment of diabetes cognitive impairment |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101574166A (en) * | 2009-06-05 | 2009-11-11 | 杨健 | Ocean deep water-containing drinking water with health care function |
-
2012
- 2012-04-18 CN CN201210114603.5A patent/CN102652758B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101574166A (en) * | 2009-06-05 | 2009-11-11 | 杨健 | Ocean deep water-containing drinking water with health care function |
Non-Patent Citations (1)
| Title |
|---|
| 吴小红.深海水的药理活性：对高脂血症的防治作用.《国外医学中医中药分册》.2005,第27卷(第1期),第39页左栏第6行至第32行. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102652758A (en) | 2012-09-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6055469B2 (en) | Algal lipid composition and method for preparing and using the same | |
| CN102250984B (en) | Method for preparing functional extracellular polysaccharide of lactic acid bacteria | |
| Ebrahimi-Mameghani et al. | The effect of Chlorella vulgaris supplementation on liver enzymes, serum glucose and lipid profile in patients with non-alcoholic fatty liver disease | |
| CN109939027A (en) | A kind of method of cosmetics stoste of the Hericium erinaceus fermentation preparation containing erythrothioneine | |
| CN102504039A (en) | Extraction process of black corn silk polysaccharide | |
| CN102652757B (en) | Application of health-care drinking liquid containing deep seawater in preparation of medicaments or health-care products for preventing or treating metabolic syndrome | |
| CN102429928B (en) | Preparation method for coix seed red yeast rice | |
| CN102652758B (en) | Application of health care drinking liquid containing deep seawater in preparation of medicaments or health care products for preventing or treating hyperlipidemia | |
| CN102409073A (en) | Method for producing antioxidant of microbial food | |
| CN103627640A (en) | Low-chlorine medium used for culturing Schizochytrium limacinum and production method for docosahexenoic acid (DHA) by using same | |
| FR3041253A1 (en) | USE OF AN OSMOTIC ISOTONIC ELECTROLYTE SOLUTION AS A CELLULAR LIFE MEDIUM IN A COSMETIC, DERMATOLOGICAL OR NUTRITIONAL COMPOSITION | |
| MX2012009678A (en) | Agents and mechanisms for treating hypercholesterolemia. | |
| JP5518891B2 (en) | Ethanol-tolerant Saccharomyces cerevisiae GP-01 by protoplast fusion, its production method, Saccharomyces cerevisiae GP-01, and method for producing yeast containing high-content organic biogermanium using highly water-soluble sodium metagermanate as germanium | |
| CN106318984B (en) | Method for preparing fatty acid glyceride by oleaginous microorganisms, glyceride and application thereof | |
| CN103865816A (en) | Yeast powder rich in selenium and germanium | |
| CN102697807B (en) | Application of health-care drinking liquid in preparation of medicines or health-care products for preventing or treating II-type diabetes mellitus, diabetes mellitus and diabetic nephropathy | |
| CN101244084A (en) | Sobering-up agent of pearl oyster meat and preparation thereof | |
| CN105326898B (en) | A kind of imitative compound immunopotentiator for apostichopus japonicus | |
| KR102235186B1 (en) | Composition for anti-inflammation comprising coconut oil with fermentation and ripening as effective component and manufacturing method thereof | |
| CN101255379A (en) | Purified fur seal oil and method for preparing the same | |
| CN104172177A (en) | Production method of food antioxidant | |
| CN103757076B (en) | A kind of method utilizing oxygen carrier raising fermentation by saccharomyces cerevisiae liquid GSH-PX activity to tire | |
| CN105400836A (en) | Production method for increasing unsaturated fatty acid content | |
| JPH01180832A (en) | Hot-water extract from fresh-water chlorella with high iodine content, production and use thereof | |
| CN104403985A (en) | Culture method of iodine-rich Nostoc flagelliforme |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |