CN102638999A - Method for producing tea extract - Google Patents

Abstract

Provided is a method for producing, from a tea material, a tea extract, which has a refreshing flavor, i.e., showing a natural, fresh and rich aroma, a clean sweetness and a clean deliciousness with little miscellaneous taste and no undesirable taste, comprising: (i) collecting aroma components from the tea material by steam distillation; (ii) enzymatically treating the distillation residue to give an enzymatically treated extract; and (iii) mixing the enzymatically treated extract obtained in (ii) with the aroma components collected in (i).

Description

The manufacturing approach of teas extract

Technical field

The present invention relates to the manufacturing approach of teas extract; Relate in more detail have nature, pure and fresh, abundant fragrance; Happy sweet taste and happy flavour are strong, and have assorted flavor less, the manufacturing approach of teas extract that can not tedious salubrious local flavor.

Background technology

Through sterilization be filled in jar or plastic bottle etc. in teas beverages such as green tea, black tea, oolong tea, tea blend owing to advocate health, obtained consumer's support energetically, show that output progressively improves.Particularly, have recently like having nature, pure and fresh, abundant fragrance, the strong and assorted flavor of happy sweet taste and happy flavour less, the tendency of beverage that can not tedious local flavor.Under this situation, the product for stable in industry and local flavor that production is in large quantities expected has descended a lot of time at the selection of employed teas raw material, the qualitative control of raw material, the cooperation ratio and the aspects such as fitting method, method for distilling of raw material.

In the manufacturing of these beverages, except above-mentioned teas raw material, usually also add the flavor improving agent of various teas extracts etc. as part of raw materials.The teas extract is from teas, only will extract and get corresponding to the neccessary composition of purpose, can modulate the quality of form corresponding to final products, local flavor etc.Thereby the interpolation of teas extract is owing to can easily obtain target effect according to the extract that the purpose of final beverage is added required type, thereby is the method for simple and performance advantageous effects in the teas drink manufacturing.

As described above, in order to enhance tea aroma or flavor tea extract for the purpose of manufacturing method, a variety of methods, for example, various proposals: in the presence of protease and tannase tea material under extracting the tea extract is characterized by the manufacturing method (Patent Document 1); contain favorite drink using the raw material obtained by steam distillation of spices (A) and the preference of the beverage material to the gas-liquid countercurrent contacting apparatus for spice obtained (B) perfume, wherein, per 1 part by weight of perfume (A) 0.01 to 100 parts by weight of fragrance contained within the range (B) (Patent Document 2); with cellulase containing at least , hemicellulase, pectinase and the original pectinase enzyme group, enzymatic decomposition of the tea extraction process embodiment is characterized by the manufacturing method of the tea extract (Patent Document 3); that the resulting green sample (bag re wrote it because specifications) Aroma enzyme compounds and / or enzyme group act on the tea extract, tea slurry, and / or enzyme-treated tea is characterized by producing tea extract (Patent Document 4); characterized by comprising the steps of a tea extract The modulation method was: green tea with warm water to prepare a slurry, the use of countercurrent contact means (SCC) process the slurry, the perfume recovered first step; addition to the extraction of tea leaves with warm water, the solid was removed after the treatment with activated carbon, Then the activated carbon was removed by filtration, to obtain a second tea extract solution step; will be obtained in the first step through the second step of spices and tea extract solution obtained in the third mixing step (Patent Document 5); through the tea leaves will steam distillation and the distillate obtained by the steam treatment, the tea extract solution obtained by mixing the tower to a method for producing a tea extract (Patent Document 6); characterized by after the step of producing a tea extract Method: (1) with 40 ℃ ~ 100 ℃ the tea warm water impregnated or wet processes; (2) to step (1) for water vapor tea extract, the step of recycling the distillate; (3) of water to the step (2 ) The distillation residue was extracted liquid recovery step of the extracted; (4) in the step (2) the distillate and step (3) a step of mixing an extract (Patent Document 7); in the tea extraction of raw materials and / or when used after extraction of polysaccharide hydrolases (from glucose, amylase, hemicellulase, pectinase, mannanase, invertase and α-galactosidase and at least one kind) for enzymatic degradation characterized processing method for producing a tea extract (Patent Document 8), etc.But; These methods all are to be main purpose with in fragrance or the taste any one; Be difficult to obtain synthetically have nature, pure and fresh, abundant fragrance, happy sweet taste and happy flavour are strong, and have assorted flavor less, teas extract that can not tedious salubrious local flavor.

The prior art document

Patent documentation

Patent documentation 1: TOHKEMY 2003-144049 communique

Patent documentation 2: TOHKEMY 2003-33137 communique

Patent documentation 3: No. 3779212 communique of Japan Patent

Patent documentation 4: International Publication brochure WO06/062133 communique

Patent documentation 5: TOHKEMY 2007-167005 communique

Patent documentation 6: TOHKEMY 2007-295921 communique

Patent documentation 7: No. 4104018 communique of Japan Patent

Patent documentation 8: TOHKEMY 2008-86280 communique.

Summary of the invention

The technical problem that invention will solve

The object of the present invention is to provide by the manufacturing of teas raw material to have nature, pure and fresh, abundant fragrance, happy sweet taste and happy flavour are strong, and have assorted flavor less, the method for teas extract that can not tedious salubrious local flavor.

The method that is used for the technical solution problem

Reclaim giving off a strong fragrance (aroma) that fragrance obtains though have nature sense, fresh feel through steam distillation, pure savoury is not enough on the whole local flavor.In addition, combination has through steam distillation reclaims in the giving off a strong fragrance extract of extract of giving off a strong fragrance that fragrance obtains and residue, and strong from bitter taste, the astringent taste of residue extract, never talkative balance is good.And, though being carried out extract that enzyme handle to obtain, tealeaves is being excellent aspect the flavor, sweet taste or flavour strengthen, and have the fragrance deficiency, drink the problem of the impression of not tea delivery fully.

The inventor etc. further investigate in order to solve above-mentioned problem; The result finds that this carries out steam distillation to tealeaves and obtains to reclaim perfume, uses the combination of certain enzyme that bottoms are handled acquisition enzyme processing extract in addition; Enzyme is handled teas extract that extract obtains with fragrant mixing of the recovery that obtains before have nature, pure and fresh, the fragrance that enriches; And happy sweet taste and happy flavour are strong; And then have assorted flavor less, can not tedious salubrious local flavor, thereby accomplished the present invention.

And; The present invention provides the manufacturing approach of teas extract, the method is characterized in that to have following operation: (i) utilize steam distillation from the teas raw material, reclaim fragrance, (ii) to bottoms carry out that enzyme handle to obtain that enzyme is handled extract, the enzyme that (iii) operation obtained in (ii) handles that the recovery that obtains in extract and the operation (i) is fragrant to be mixed.

The invention effect

The teas extract that is obtained by the present invention has nature, pure and fresh, abundant fragrance, and happy sweet taste and happy flavour are strong, so have assorted flavor less, can not tedious salubrious local flavor.In addition, through adding the teas extract that obtains by the present invention when the teas drink manufacturing, can give nature, pure and fresh, abundant fragrance to beverage with simple method, and hide bitter taste or astringent taste, give happy sweet taste, happy flavour.

The specific embodiment

As the teas raw material that uses among the present invention, can enumerate evergreen tree by Theaceae and be tea (formal name used at school: the leave that bud Camellia sinensis (L) O.Kuntze), leaf, stem etc. obtain, through azymic tea, semi-fermented tea and the fermented tea of tea making.Can enumerate green tea (simmer tea, beautiful reveal, hat tea, thick tea, jade green tea, smear tea, roasting tea, pot parched tea, stone roller tea etc.) as azymic tea; Can enumerate oolong tea, Paochung tea etc. as semi-fermented tea; Can enumerate black tea, Pu'er tea etc. as fermented tea.In addition, also can use the tea etc. that azymic tea or semi-fermented tea has been carried out perfuming with spending.Wherein, especially preferably pure and fresh, natural fragrance or sweet taste, flavour etc. are necessary green tea, oolong tea, jasmine tea etc.In addition; As required, as auxiliary material also can add for example cure barley (wheat tea), cure Fructus Hordei Germinatus, cure heart of a lotus seed benevolence (heart of a lotus seed benevolence tea), cure rice, cure brown rice, cure germinated rice, cure the buckwheat seed (buckwheat tea), cure corn, roasted sesame, cure the lamb's-quarters wheat, cure three-coloured amaranth, cure maiden cane, cure barnyard grass, cure millet, cure cereal such as soybean; Salvia japonica; Thyme; Marjoram; Wild marjoram; Sweet basil; Peppermint; Purple perilla; Balm; Verbena officinalis; Savoury; Rosemary; Lemon grass (Cymbopogon citratus); The blueberry leaf; Basyleave; Ilex paraguarensis; Eucalyptus leaves; Sassafrases; Santal; Absinth; River deer tooth dish; Pimiento; Chinese cassia tree; Alate senna leaf; Anistree; Mustard; The West mustard (horseradish); The water mustard; Mustard; Black tonka-bean; Fenugreek; Chinese pepper; Black pepper; White pepper; Allspice; Flesh fruit; Nutmeg; Cloves; Chinese celery; Radix Angelicae Sinensis; Chervil; Anise; Fennel; Tarragon; Caraway; Fennel seeds; Dill; Caraway seed; Galangal; Cardamom; Ginger; Curcuma zedoary; Turmeric (root tuber of aromatic turmeric); Vanilla; The needle juniper fruit; Wintergreen; Chamomile; The Rome chamomile; Chrysanthemum; Lavender; Shrub althea flower; Safron; Pot marigold; Flores aurantii; Violet; Rose hip; Hawthorn; Longan; The fruit of Chinese wolfberry; Sundew (youthwort); Orange peel; Lemon peel; Root of hollyhock; The Radix Ginseng; Tienchi-Ginseng; Wilsonii; Gymnema sylvestre; The Louis boss; The fine and soft tea of vertebra; The bark of eucommia; Cordate houttuynia; Cassia seed; Haab tea; Gynostemma pentaphylla tea; Plantain seed tea; Oriental cherry tea; Soil Changshan leaf tea; Persimmon leaf tea; Kelp tea; Pine needle tea; Angelica keiskei tea; Guava tea; Loguat leaf tea; Aloe tea; Root tuber of aromatic turmeric tea; China fir dish tea; Safflower tea; Comfrey tea; Radix Angelicae Sinensis tea; Argy wormwood tea; Ginkgo leaf tea; Hua Licha; Mulberry-leaf Tea; Burdock tea Aralia wood tea; Dandelion herb tea; Sword bean tea; Elder tea; Bitter leaves tea; Loguat leaf tea; Comospore maple tea; The leaf of each kind of plant such as Momordica grosvenori; Stem; Root etc.

Among the present invention, at first in the 1st operation (i), reclaim fragrance by the teas raw material through steam distillation.After utilizing this operation to obtain recovery perfume (or spice), be added into through in the enzyme processing teas extract that operation obtains afterwards reclaiming perfume, thereby obtain to have pure and fresh, natural fragrance and have sweet taste and the teas extract of flavour.

As the method for steam distillation, for example can adopt teas raw material pulverizing to suitable granularity, be mixed and made into slurry with water, utilize the gas-liquid counter current contact method that this slurry is handled, carry out the method that fragrance reclaims; With the teas raw material directly or be filled into after pulverizing in the container such as tower, transporting water steam in tower makes the teas raw material contact with water vapour, makes the condensing and method that reclaims of water vapour after the contact etc.Preferred especially gas-liquid counter current contact extraction method.

Gas-liquid counter current contact extraction method can be implemented through itself known the whole bag of tricks, the method that the device that for example can adopt the special fair 7-22646 communique of use Japan to be put down in writing extracts.When the method that this device reclaims fragrance is used in explanation particularly; Can enumerate having alternate combinations has rotating cone to contact on the rotating cone of extraction element with the fixing gas-liquid counter current of the structure of circular cone; The tea raw material of aqueous or pasty state is flowed down from top; Steam is risen, the method that the fragrance component that the teas raw material is existed originally reclaims from the bottom.The operating condition of this gas-liquid counter current contact extraction element can at random be selected according to the intensity of the kind of the disposal ability of this device, raw material and concentration, fragrance etc.The teas raw material in the teas raw material slurry and the ratio of water are so long as teas raw material slurry is in the amount with mobile state; Then can adopt any ratio; With respect to teas raw material 1 mass parts, can enumerate～30 times of amounts of 5 times of amounts of water usually, preferably can enumerate in the scope of～20 times of amounts of 8 times of amounts of water.When water is lower than this scope, then be difficult to occur mobile, water breaks away from this scope when a lot of in addition, the tendency that then has the fragrance of gained distillate to weaken.

Enumerate one of the operating condition example of gas-liquid counter current contact extraction element, be described below.

Raw material supplying speed: 300～700L/hr

Vapor flow rate: 5～50kg/hr

Evaporation capacity: 3～35kg/hr

Column bottom temperature: 40～100 ℃

Tower upper temp: 40～100 ℃

Vacuum: 1.3KPa～atmospheric pressure.

In addition; The steam distillation that utilizes tower to carry out is steam to be fed be filled in the teas raw material of tower; Make and follow fragrance component that water vapour distillates, can adopt any distillation means of pressurized steam distillation, water at atmospheric pressure steam distillation, reduced steam distillation with the condensing method of water vapour.Specifically, for example the bottom from steam distillation still that the teas raw material is housed is blown into water vapour, utilizes to be connected in the cooler that top distillates side and will to distillate steam cooled, can catch the distillate that contains the volatile aroma composition with the form of condensate.As required, connect the cold-trap that has used refrigerant, can also positively more lower boiling volatile aroma composition be caught through front end at this fragrance acquisition equipment.In addition, owing to can prevent fragrance component effectively because the deterioration that caused of heating, thereby preferably in the presence of anti-oxidants such as inert gas such as nitrogen and/or vitamin C, carry out steam distillation.

The 2nd operation according to the present invention (ii) is the steam distillation residue that produces in the 1st operation (i) to be carried out enzyme handle, obtain the operation that enzyme is handled extract.The enzyme processing for example can be added enzyme and carry out in the mixture of steam distillation residue and water.

When utilizing gas-liquid counter current contact extraction method to reclaim fragrance,, thereby can be cooled to and be suitable for the enzyme treatment temperature, directly add enzyme because bottoms have become the pulp-like that contains extract as steam distillation.In addition; During for the residue of tower steam distillation; As the water that is treated to necessary amount for enzyme, can be generally 1 mass parts～100 mass parts with respect to residuum feedstocks 1 mass parts, be preferably 5 mass parts～water of 50 mass parts is added in the tower, carries out enzyme reaction under the condition stirring or leave standstill.

Then, in the mixture of water and bottoms, add enzyme and carry out the enzyme processing.Owing in the system that contains teas raw material itself, carry out enzyme and handle, thereby the tissue of bottoms decomposes, produces in a large number taste composition, can obtain sweet taste, flavour etc. and be the strong extract of distinguishing the flavor of among the present invention.

Enzyme as can be used for this enzyme processing does not have special qualification, for example can enumerate saccharic catabolic enzyme, protease, lipase, tannase, chlorogenic acid esterase etc.And then; As the saccharic catabolic enzyme, can enumerate amylase, glucoamylase, Pullulanase, cellulase, hemicellulase, zytase, pectase, arabanase, dextranase, dextranase, mannase, alpha-galactosidase etc. particularly.These enzymes use through appropriate combination, can strengthen sweet taste or flavour that enzyme is handled extract.Particularly; From the viewpoint that flavour strengthens, sweet taste strengthens, the combination of combination, (3) glucoamylase and the pectase of combination, (2) glucoamylase and the hemicellulase of preferred (1) protease and tannase or the combination of (4) glucoamylase and cellulase.

Contain 25% the protein (with reference to japanese food standard analysis table the five editions) of having an appointment in the tealeaves, infer it and form that also basic in the steam distillation residue what do not change is equal extent.With protease this protein is decomposed, then can obtain the strong teas extract of flavour, but since the protein in the tealeaves combine with tannic acid, even if thereby make protease act on bottoms individually, basically can not produce amino acid yet.But, acting on bottoms through making protease and tannase, the part of the protein in the bottoms is decomposed, acquisition flavour or the teas extract that pure savoury is strong, astringent taste is few.

In the combination of saccharic catabolic enzyme; The combination of the combination of described (2) glucoamylase through using and hemicellulase, (3) glucoamylase and pectase or (4) are though that the combination of glucoamylase and cellulase is handled its reason of teas extract that is obtained to the steam distillation residue is not clear; But it is owing to ground outside its sweet taste expectation far is better than the teas extract of the combination acquisition of using other saccharic catabolic enzymes, so preferred especially.

Tannase is the enzyme with the depside key hydrolysis of the hydroxy ester bonding in gallic acid and the tannic acid, for example is the enzyme that L-Epicatechin gallate is hydrolyzed into epigallocatechin and gallic acid.As the tannase that can use among the present invention; For example can enumerate particularly the tannic acid enzyme-producing bacteria that will belong to Eurotium, Penicillium, rhizopus, Rhizomucor, lactobacillus, staphylococcus, streptococcus, Long Paien Pseudomonas (Lonepinella spp.) etc. according to conventional methods cultivate with these der Pilzs in normally used culture medium carry out solid culture or Liquid Culture, utilize conventional method the culture of gained or its handled thing to be carried out purification process and the tannase that obtains.In addition, commercially available tannase for example also can use tannase (500U/g; キ ッ コ ー マ Application corporate system), tannase (5,000U/g; キ ッ コ ー マ Application corporate system), tannase (500U/g; The フ ー ズ of Mitsubishi Chemical corporate system) etc.The use amount of tannase is owing to tire and wait and can't lump together, and the quality with the initial teas raw material of bottoms is a benchmark usually, can enumerate usually be about 0.1～about 50U/g, preferably in the scope of about 0.5～about 20U/g.

Protease is the enzyme with the peptide bond hydrolysis of protein or peptide.As the protease that can use among the present invention, for example can enumerate protease A, protease M, protease P, delicate flavour enzyme, peptase R, Newlase (registration mark) A, Newlase (registration mark) F (protease that aspergillus bacterium above, day wild エ Application ザ イ system corporate system is originated); Sumizyme (registration mark) AP, Sumizyme (registration mark) LP, Sumizyme (registration mark) MP, Sumizyme (registration mark) FP, Sumizyme (registration mark) LPL (protease in the aspergillus bacterium source of above, new Japanese chemical industrial company system); Protin (registration mark) FN (big protease of originating) with the aspergillus bacterium that changes into corporate system; Denapsin2P, Denatyme (registration mark) AP, XP-415 (protease that aspergillus bacterium above, Na ガ セ ケ system テ ッ Network ス corporate system is originated); Orientase (registration mark) 20A, Orientase (registration mark) ONS, Tetrase (registration mark) S (protease that aspergillus bacterium above, エ イ チ PVC ィ ア イ corporate system is originated); Molsin (registration mark) F, PD enzyme, IP enzyme, AO-protease (protease that aspergillus bacterium above, キ ッ コ ー マ Application corporate system is originated); サ カ ナ ー ゼ (protease in the aspergillus bacterium source of scientific research Off ァ Le マ corporate system); パ Application チ ダ ー ゼ (registration mark) YP-SS, パ Application チ ダ ー ゼ (registration mark) NP-2, パ Application チ ダ ー ゼ (registration mark) P (protease that aspergillus bacterium above, ヤ Network Le ト pharmaceutical industries corporate system is originated); Flavourzyme (registration mark) (protease in the aspergillus bacterium source of ノ ボ ザ イ system ズ ジ ャ パ Application corporate system); Kokulase (registration mark) SS, Kokulase (registration mark) P (protease that aspergillus bacterium above, three common ラ イ Off テ ッ Network corporate systems are originated); VERON PS, COROLASE PN-L (protease that aspergillus bacterium above, AB エ Application ザ イ system corporate system is originated); Protease N, protease N L, protease S, プ ロ レ ザ ー (registration mark) FG-F (protease of the bacterial origin of above, ア マ ノ エ Application ザ イ system corporate system); ProtinP, デ ス キ Application, デ ピ レ イ ス, ProtinA, サ モ ア ー ゼ (registration mark) (above, big and change into the protease of the bacterial origin of corporate system); Bioprase (registration mark) XL-416F, Bioprase (registration mark) SP-4FG, Bioprase (registration mark) SP-15FG (protease of the bacterial origin of above, Na ガ セ ケ system テ ッ Network ス corporate system); Orientase (registration mark) 90N, ヌ Network レ イ シ Application (registration mark), Orientase (registration mark) 10NL, Orientase (registration mark) 22BF (protease of the bacterial origin of above, エ イ チ PVC ィ ア イ corporate system); ア ロ ア ー ゼ (registration mark) AP-10 (protease of the bacterial origin of ヤ Network Le ト pharmaceutical industries corporate system); Protamex (registration mark), neutral proteinase (registration mark), alkaline enzyme (registration mark) (protease of the bacterial origin of above, ノ ボ ザ イ system ズ corporate system); COROLASE N, COROLASE 7089, VERON W, VERON P (protease of the bacterial origin of above, AB エ Application ザ イ system corporate system); エ Application チ ロ Application NBS (Luo Dong changes into the protease of the bacterial origin of industrial group's system); Alkali protease GL440, ピ ュ ラ Off ェ Network ト (registration mark) 4000L, proteinase 8 99, プ ロ テ ッ Network ス 6L (protease of the bacterial origin of above, ジ ェ ネ コ Application consonance corporate system); Pronase (registration mark) AS, pronase (registration mark) AF (protease that actinomyces above, scientific research Off ァ Le マ corporate system are originated); タ シ ナ ー ゼ (registration mark) (protease in the actinomyces source of ジ ェ ネ Application コ ア consonance corporate system); Papain W-40 (protease of the plant origin of ア マ ノ エ Application ザ イ system corporate system); Food is with refining papain (protease of the plant origin of Na ガ セ ケ system テ ッ Network ス corporate system); Can enumerate pepsin, trypsase of animal origin etc. in addition.Difference cannot treat different things as the same the use amount of these protease with the difference of tiring etc., but is benchmark with the quality of the initial teas raw material of bottoms usually, can enumerate about 0.01U/g～about 100U/g usually, preferably in the scope of about 1U/g～about 80U/g.

As the enzyme treatment conditions that combination constituted by protease and tannase; For example with respect to (1) to the slurry of teas carry out after the gas-liquid counter current contact is handled the steam distillation residue slurry perhaps (2) carried out bottoms 1 mass parts that produced behind the steam distillation with tower; Add the water of common 8～50 mass parts, preferred 10～20 mass parts; Sterilization is cooled off after about 2 seconds～about 20 minutes under about 60～about 121 ℃; In the product of gained, add protease and tannase, under about 20～about 60 ℃, carry out about 30 minutes～about 24 hours enzyme and handle.After enzyme is handled, about 60～about 121 ℃ down heating about 2 the second～made enzyme deactivation in about 20 minutes, cool off afterwards, implement Separation of Solid and Liquid, filtration, handle extract thereby can obtain enzyme.

Amylase is through with the glycosidic bond hydrolysis and convert the amylose in the starch or amylopectin the enzyme of glucose, maltose and compound sugar etc. to, and amylase comprises AMS, beta amylase, glucoamylase.

AMS is the enzyme that the α of starch or glycogen-1,4 key is cut off, produces polysaccharide or compound sugar brokenly.Beta amylase is the enzyme that starch or decomposition of glycogen is become maltose.Glucoamylase is with the decomposition of the α of the non-reduced end of sugar chain-1,4 key, glucogenic enzyme.

For example can enumerate グ Le Network (registration mark) SG, グ Le Network ザ イ system (registration mark) AF6, グ Le Network ザ イ system (registration mark) NL4.2, brew alcoholic beverages as commercially available article in the glucoamylase with glucoamylase " ア マ ノ " SD (above, day wild エ Application ザ イ system corporate system); GODO-ANGH (contract alcohol corporate system); Kokulase (registration mark) G2, Kokulase (registration mark) M (above, the フ ー ズ of Mitsubishi Chemical corporate system); オ プ チ デ ッ Network ス L (ジ ェ ネ Application コ ア coordinate corporate system); Sumizyme (registration mark), Sumizyme (registration mark) SG (above, new Japanese chemical industrial company system); グ Le コ チ ー system (registration mark) #20000 (Na ガ セ ケ system テ ッ Network ス corporate system); AMG, サ ン ス ー パ ー (above, ノ ボ ザ イ system ズ ジ ャ パ Application corporate system); グ ル タ ー ゼ AN (エ イ チ PVC ィ ア イ corporate system); ユ ニ ア ー ゼ (registration mark) K, ユ ニ ア ー ゼ (registration mark) 2K, ユ ニ ア ー ゼ (registration mark) 30, ユ ニ ア ー ゼ (registration mark) 60F (above, ヤ Network Le ト pharmaceutical industries corporate system); マ グ Na ッ Network ス (registration mark) JW-201 (Luo Dong changes into industrial group's system); グ リ Application De ア ミ Le (registration mark) AG (ダ ニ ス コ ジ ャ パ Application corporate system) etc.The use amount of glucoamylase is a benchmark with the quality of the initial teas raw material of bottoms, can enumerate to be about 0.1U/g～about 1 000U/g, preferably in the scope of about 1U/g～about 100U/g usually.

Pectase also is known as polygalacturonase, decompose pectin enzyme, polygalacturonase, pectin removes polymerase, is the enzyme with α-1, the 4 key hydrolysis of pectinic acid, pectin, pectic acid etc.Pectase is known to be contained in bacterium, mould, yeast, higher plant, snail etc., can be widely used among the present invention from these pectases for gathering the biology of representative.In addition, can also use commercially available pectase preparation.As commercially available pectase preparation, for example can enumerate ス ク ラ ー ゼ (registration mark) A, ス ク ラ ー ゼ (registration mark) N, ス ク ラ ー ゼ (registration mark) S (above, the フ ー ズ of Mitsubishi Chemical corporate system), Pectinex Ultra (registration mark) SP-L (ノ ボ ノ Le デ ィ Network ス A/S corporate system), Meicelase (registration mark) (Meiji Seika Kaisba (strain) corporate system), Ultrazyme (registration mark) (ノ ボ ノ Le デ ィ Network ス A/S corporate system), NewlaseF (registration mark) (day wild エ Application ザ イ system (strain) corporate system) etc.The use amount of pectase is because pectase preparation contains multiple type enzyme usually; Thereby be difficult to represent with active unit; With respect to the initial teas raw material of bottoms, can enumerate usually in the scope of about 0.01 quality %～about 5 quality %, preferred 0.1 quality %～about 2 quality %.

Cellulase is with β-1, the enzyme of the glycosidic bond hydrolysis of 4-glucan (for example cellulose).Cellulose is a D-glucose has a kind of of polysaccharide that branch ground do not connect through β-1,4 key, and the quantity of glucose allegedly has 5000 approximately.Cellulose is the main composition composition of the cell membrane of plant, and hydrophily by force but water insoluble.Exist in the cellulase from intramolecule and decompose the exoglucanase (cellobiohydrolase) that cellobiose is free with the endoglucanase of cellulose cut-out with by the reduction end of sugar chain and any one of non-reduced end.In addition, be mixed with beta-glucosidase, glucose is free in the commercially available cellulose enzyme more.As operable cellulase among the present invention; Then there is not special qualification so long as have cellulolytic activity; Can use cellulase arbitrarily; As commercially available cellulase preparation, for example can enumerate cellulase T " ア マ ノ ", cellulase A " ア マ ノ " (above, day wild エ Application ザ イ system corporate system); De リ セ ラ ー ゼ (registration mark) KSM, マ Le チ Off ェ Network ト (registration mark) A40, cellulase GC220 (above, ジ ェ ネ Application コ ア coordinate corporate system); Cellulase GODO-TCL, cellulase GODO TCD-H, ベ ッ セ レ ッ Network ス (registration mark), cellulase GODO-ACD (above, contract alcohol corporate system); Cellulase (Japan's textile company system); セ Le ラ イ ザ ー (registration mark), cellulase XL-522 (above, Na ガ セ ケ system テ ッ Network ス corporate system); セ Le ソ Off ト (registration mark), デ ニ マ ッ Network ス (registration mark) (above, ノ ボ ザ イ system ズ corporate system); Cellulase (registration mark) AC40, cellulase (registration mark) AL, cellulase (registration mark) T2 (above, エ イ チ PVC ィ ア イ corporate system); Cellulase " オ ノ ズ カ " 3S, cellulase Y-NC (above, ヤ Network Le ト pharmaceutical industries corporate system); Sumizyme (registration mark) AC, Sumizyme (registration mark) C (above, new Japanese chemical industrial company system); エ Application チ ロ Application CM, エ Application チ ロ Application MCH, バ イ オ ヒ ッ ト (Luo Dong changes into industrial group's system) etc.The use amount of cellulase is owing to contain multiple type enzyme usually in the commercially available cellulase preparation; Thereby be difficult to represent with active unit; With respect to the initial teas raw material of bottoms, can enumerate usually in the scope of about 0.01 quality %～about 5 quality %, preferred about 0.1 quality %～about 2 quality %.

Hemicellulase is the enzyme that hemicellulose is decomposed.Hemicellulose is to constitute in the polysaccharide of cell membrane of land plant cell except cellulose and the material the pectin, and the sugar of formation is diversified, and the bonding mode is complicacy also.And, have with cellulose the effect that forms hydrogen bond, forms reinforcing cell membranes such as covalent bond with lignin.Sugar with side chain etc. is bonded in as the structure on the sugar of the main chain of skeleton, and is very many to the kind of its hemicellulase that decomposes.As hemicellulase; For example dextranase, mannase, alpha-galactosidase, Galactanase, zytase, arabinase, polygalacturonase etc. can be enumerated, enzyme can also be adopted with a plurality of activity that these multiple type sugared keys are decomposed.As commercially available hemicellulase, for example can enumerate hemicellulase " ア マ ノ " (amano pharmaceutical corporate system), ベ イ Network ザ イ system (registration mark) HS2000, ベ イ Network ザ イ system (registration mark) IConc (above, Japanese シ イ ベ Le ヘ グ Na ー corporate system), エ Application チ ロ Application LQ (Luo Dong changes into industrial group's system), cellulase (registration mark) HC100, cellulase (registration mark) HC, cellulase (registration mark) TP25, cellulase (registration mark) B, hemicellulase M (above, エ イ チ PVC ィ ア イ corporate system), Sumizyme (registration mark) X (new Japanese chemical industrial company system), VERON191, VERON393 (above, レ ー system エ Application ザ イ system corporate system) etc.The use amount of hemicellulase is owing to contain multiple type enzyme usually in the commercially available hemicellulose enzyme preparation; Thereby be difficult to represent with active unit; With respect to the initial teas raw material of bottoms, can enumerate usually in the scope of about 0.01 quality %～about 5 quality %, preferred about 0.1 quality %～about 2 quality %.

Among the present invention, there is not invertase activity in the activity of also preferred employed saccharic catabolic enzyme in fact.Usually contain the to a certain degree sucrose of amount in the teas raw material more.In addition, when saccharic catabolic enzyme combination is played a role, can not negate that sucrose decomposes from polysaccharide the possibility of dissociating takes place after coming out a little.Among the present invention, as stated, through the combination of saccharic catabolic enzyme, the glucose in the teas extract increases in large quantities, if but at this moment with sucrose decomposition, then can the pleasantly sweet negative effect that reduces a little and then produce tart flavour or assorted flavor.Therefore, the enzyme that uses among the present invention does not preferably have invertase activity in fact in its activity.Whether exist the invertase activity can be as judging in the employed enzyme preparation in fact: with sucrose be that substrate plays a role enzyme, confirms the generation of glucose, and then judges.Explain that the generation of glucose can use commercially available glucose test paper to confirm.

As the condition that enzyme is handled, can adopt the common enzyme treatment conditions of corresponding used enzyme.For example; With respect to (1) to the slurry of teas carry out after the gas-liquid counter current contact is handled the steam distillation residue slurry perhaps (2) carried out bottoms 1 mass parts that produced behind the steam distillation with tower; Add the water of common 8～50 mass parts, preferred 10～20 mass parts; About 60～about 121 ℃ down sterilization about 2 the second～cool off after about 20 minutes; In the product of gained, add the enzyme of necessity of ormal weight, usually in pH3～6, preferred pH4～5.5 times stirs or leaves standstill, thereby can carry out enzyme reaction.In order to prevent the oxidative degradation in the enzyme reaction, can add ascorbic acid or sodium ascorbate about 10ppm～500ppm with respect to the slurry total amount.Enzyme there is no need under the optimum temperature of enzyme, to react; The situation that under low temperature slightly, makes its reaction is also arranged preferably, and the temperature of enzyme reaction can be enumerated in about 20 ℃～about 70 ℃ usually, preferred about 25 ℃～about 60 ℃, the preferred about 30 ℃ especially～about 50 ℃ scope.In addition, the reaction time can be common 5 minutes～24 hours, preferred 1 hour～20 hours, more preferably 4 hours～18 hours.Among the present invention; Under the low temperature enzyme is reacted slightly in order in the teas extract, not produce assorted flavor; Thereby the situation that needs the long period arranged as the reaction time; But the carrying out that also can react on one side through the growing amount affirmation of HPLC the analysiss mensuration glucose of etc.ing, Yi Bian determine the reaction time, carry out enzyme append interpolation etc.After enzyme is handled, through about 60 ℃～about 121 ℃ down heating about 2 the second～made enzyme deactivation in about 20 minutes, cool off afterwards, and then carry out Separation of Solid and Liquid, filtration, handle extract thereby can obtain enzyme.

The enzyme that so obtains is handled extract and can be concentrated as required.As method for concentration, for example can enumerate concentrated, the anti-suitable concentrated means such as film (RO film) concentrates, freeze concentration of soaking into of decompression, through concentrating, can obtain to handle the concentrate of extract according to enzyme of the present invention.The degree that concentrates does not have special qualification, be generally Bx3 °～Bx80 °, be preferably Bx8 °～Bx60 °, more preferably Bx10 °～scope of Bx50 ° in.

The 3rd operation of the present invention (iii) is that the (ii) middle enzyme that obtains of recovery perfume that obtains in the 1st operation (i) and the 2nd operation is handled the operation that extract mixes, obtains the teas extract.The fragrant mixed proportion of handling extract with enzyme of the recovery of this moment can be handled the drink flavours in food products or the target local flavor of extract and freely select according to being added with enzyme of the present invention, the mass ratio that the fragrant enzyme that obtains in (ii) with the 2nd operation of the recovery that obtains in the 1st operation (i) is handled extract usually can for 1: 10～10: 1, be preferably 1: 5～5: 1, in more preferably 1: 3～3: 1 the scope.Reclaim fragrant mass ratio and surpass at 10: 1 o'clock, then sweet taste is not enough; And the mass ratio that the (ii) middle enzyme that obtains of the 2nd operation is handled extract surpasses at 1: 10 o'clock, and then pure and fresh and natural fragrance is not enough.In addition, sweet taste and flavour have the tendency that when both balances are lucky, then becomes good.

The mixed liquor (teas extract) that the 3rd operation obtains in (iii) can directly be processed product, also can precipitate to remove, filter, be filled into after the operation such as sterilization and become negotiable state in the closed container.

Teas extract of the present invention is through making an addition in the diet article such as green tea beverage, oolong tea beverage, black tea drinks or blended tea beverage in the scope with common about 0.01 quality %～about 2 quality %; Can give these diet article natures, pure and fresh, abundant fragrance; And hide bitter taste or astringent taste, and give happy sweet taste, happy flavour.

Below utilize embodiment, comparative example and reference example that the present invention is described more particularly, but these embodiment, comparative example and reference example are merely example, do not limit the scope of the invention.

Embodiment 1

(utilizing gas-liquid counter current contact extraction method that green tea is carried out the example that fragrance reclaims, bottoms carried out protease and tannase processing)

Using hammer-mill (screen cloth 1.2mm) that commercially available Shizuoka is produced a tea 40kg pulverizes; Add the water 360kg that is dissolved with sodium ascorbate 0.24kg; Process slurry form, utilize gas-liquid counter current contact extraction method obtaining to reclaim fragrant 16kg (with respect to green tea 40%) (below be called reclaim fragrant (a)) under the following condition.

A device: SCC Model1000! フ レ ー バ ー テ ッ Network corporate system)

Treatment conditions

Raw material supplying speed: 700L/Hr

Vapor flow rate: 55kg/hr

Bar tape speed: about 4%

Column bottom temperature: 100 ℃

Tower upper temp: 100 ℃

Vacuum: atmospheric pressure.

Residue slurry 400kg (being equivalent to green tea 40kg) (below be called residue slurry (b)) is cooled to 45 ℃; Gather 2kg (being equivalent to green tea 200g) wherein equably, reach 90 ℃ of temperature sterilizations after, be cooled to 40 ℃ immediately; Interpolation protease M (5,500U/g; ア マ ノ エ Application ザ イ system (strain)) 2g and tannase (500U/g; キ ッ コ ー マ Application (strain)) 2g stirred 30 minutes under uniform temp, and after fully enzyme being mixed, standing and reacting is 16 hours under uniform temp.Through after the reaction time, add sodium ascorbate 0.5g and mix fully, carry out Separation of Solid and Liquid afterwards; Heating made enzyme deactivation in 1 minute under 90 ℃; After being cooled to 20 ℃, use is coated with diatomaceous suction filter in advance and filters, and obtains the filtrating 1721g (Bx7.2 °, pH4.6) of clarification.Use Rotary Evaporators that filtrate decompression is concentrated, obtain Bx50.0 ° concentrate 247.8g.Then, in concentrate, add to reclaim fragrant (a) 82.6g (the Bx conversion solid constituent of concentrate 2/3 amount) and sodium ascorbate 0.46g (the Bx conversion solid constituent of concentrate 0.4% amount), fully mix back interpolation water, Bx is adjusted to 35 °.Then,, be cooled to 30 ℃, be filled in the container, obtain Bx35 ° green-tea extract (the present invention's article 1) 333.3g (pH4.9, with respect to green tea yield 167.5%) 90 ℃ of down heating 1 minute.

Comparative example 1

(green tea is not carried out fragrance and reclaims, also do not carry out the example that enzyme is handled)

Use hammer-mill (screen cloth 1.2mm) that commercially available Shizuoka is produced a tea 200g and pulverize, add the water 3600g that is dissolved with sodium ascorbate 2.4g, process slurry form; Be heated to 90 ℃ and extract after 10 minutes, be cooled to 30 ℃ immediately, carry out Separation of Solid and Liquid; 90 ℃ of following heat sterilizations 1 minute; After being cooled to 20 ℃, use is coated with diatomaceous suction filter in advance and filters, and obtains the filtrating 1688g (Bx4.2 °, pH5.3) of clarification.Use Rotary Evaporators that filtrate decompression is concentrated, obtain Bx35 ° concentrate 202.5g.Then,, be cooled to 30 ℃, be filled in the container, obtain Bx35 ° green-tea extract (relatively article 1) 202.5g (pH5.3, with respect to green tea yield 101.3%) 90 ℃ of down heating 1 minute.

Comparative example 2

(green tea not being carried out the example that fragrance reclaimed, carried out protease and tannase processing)

Use Shizuoka that hammer-mill (screen cloth 1.2mm) will be commercially available to produce a tea 200g and pulverize, add the water 3600g that is dissolved with sodium ascorbate 2.4g, process slurry form, reach 90 ℃ of temperature sterilizations after, be cooled to 40 ℃ immediately, interpolation protease M (5,500U/g; ア マ ノ エ Application ザ イ system (strain)) 2g and tannase (500U/g; キ ッ コ ー マ Application (strain)) 2g stirred 30 minutes under uniform temp, and after fully enzyme being mixed, standing and reacting is 16 hours under uniform temp.Through after the reaction time, add sodium ascorbate 0.5g and mix fully, carry out Separation of Solid and Liquid afterwards; Heated 1 minute down at 90 ℃; After being cooled to 20 ℃, use is coated with diatomaceous suction filter in advance and filters, and obtains the filtrating 1721g (Bx7.2 °, pH4.6) of clarification.Use Rotary Evaporators that filtrate decompression is concentrated, obtain Bx35 ° concentrate 325.9g.Then,, be cooled to 30 ℃, be filled in the container, obtain Bx35 ° green-tea extract (relatively article 2) 325.9g (pH4.9, with respect to green tea yield 163.0%) 90 ℃ of down heating 1 minute.

Comparative example 3

(utilizing gas-liquid counter current contact extraction method that green tea is carried out fragrance reclaims, bottoms is not carried out the example that the direct enforcement of enzyme processing is extracted)

Gather residue slurry (b) 2kg (being equivalent to green tea 200g) that embodiment 1 obtains equably, reach 90 ℃ of temperature sterilizations after, be cooled to 40 ℃ immediately; Adding sodium ascorbate 0.5g mixes fully; Carry out Separation of Solid and Liquid afterwards, 90 ℃ of down heating 1 minute, be cooled to 20 ℃ after; Use is coated with diatomaceous suction filter in advance and filters, and obtains the filtrating 1678g (Bx4.1 °, pH5.2) of clarification.Use Rotary Evaporators that filtrate decompression is concentrated, obtain Bx50.0 ° concentrate 138.0g.Then, in concentrate, add to reclaim fragrant (a) 46.0g (the Bx conversion solid constituent of concentrate 2/3 amount) and sodium ascorbate 0.28g (the Bx conversion solid constituent of concentrate 0.4% amount), fully mix back interpolation water, Bx is adjusted to 35 °.Then,, be cooled to 30 ℃, be filled in the container, obtain Bx35 ° green-tea extract (relatively article 3) 197.1g (pH5.5, with respect to green tea yield 98.5%) 90 ℃ of down heating 1 minute.

Embodiment 2

(enzyme of embodiment 1 being replaced to the example of glucoamylase and hemicellulase)

In embodiment 1, use Sumizyme (glucose-amylase activity 2,000U/g as enzyme; The glucoamylase of new Japanese chemical industrial company system) 2g and Sumizyme X (hemicellulase of new Japanese chemical industrial company system) 2g; In addition carry out 1 identical operation, obtain Bx35 ° green-tea extract (the present invention's article 2) 325.8g (pH5.3, with respect to green tea yield 162.9%) with embodiment.

Embodiment 3

(enzyme of embodiment 1 being replaced to the example of glucoamylase and pectase)

In embodiment 1, use Sumizyme (glucose-amylase activity 2,000U/g as enzyme; The glucoamylase of new Japanese chemical industrial company system) 2g and ス Network ラ ー ゼ N (pectase of the Off ー ズ of Mitsubishi Chemical corporate system) 2g; In addition carry out 1 identical operation, obtain Bx35 ° green-tea extract (the present invention's article 3) 328.4g (pH5.3, with respect to green tea yield 164.2%) with embodiment.

Embodiment 4

(enzyme of embodiment 1 being replaced to the example of glucoamylase and cellulase)

In embodiment 1, use Sumizyme (glucose-amylase activity 2,000U/g as enzyme; The glucoamylase of new Japanese chemical industrial company system) 2g and cellulase T " ア マ ノ " (cellulase of day wild エ Application ザ イ system corporate system) 2g; In addition carry out 1 identical operation, obtain Bx35 ° green-tea extract (the present invention's article 4) 318.7g (pH5.3, with respect to green tea yield 159.4%) with embodiment.

Embodiment 5

(enzyme of embodiment 1 only being replaced to the example of tannase)

In embodiment 1, use tannase (500U/g as enzyme; キ ッ コ ー マ Application (strain)) in addition 2g carries out the 1 identical operation with embodiment, obtains Bx35 ° green-tea extract (the present invention's article 5) 241.0g (pH5.3, with respect to green tea yield 120.5%).

Embodiment 6

(enzyme of embodiment 1 is only replaced to examples of proteases)

In embodiment 1, use protease M (ア マ ノ エ Application ザ イ system (strain)) 2g as enzyme; In addition carry out 1 identical operation, obtain Bx35 ° green-tea extract (the present invention's article 6) 321.8g (pH5.3, with respect to green tea yield 160.9%) with embodiment.

Embodiment 7

(enzyme of embodiment 1 only being replaced to the example of glucoamylase)

In embodiment 1 as enzyme use Sumizyme (2,000U/g; The glucoamylase of new Japanese chemical industrial company system) in addition 2g carries out the 1 identical operation with embodiment, obtains Bx35 ° green-tea extract (the present invention's article 7) 268.4g (pH5.3, with respect to green tea yield 134.2%).

Embodiment 8

(enzyme of embodiment 1 only being replaced to the example of hemicellulase)

In embodiment 1, use Sumizyme X (hemicellulase of new Japanese chemical industrial company system) 2g as enzyme; In addition carry out 1 identical operation, obtain Bx35 ° green-tea extract (the present invention's article 8) 322.4g (pH5.3, with respect to green tea yield 131.2%) with embodiment.

Embodiment 9

(enzyme of embodiment 1 only being replaced to the example of pectase)

In embodiment 1, use ス ク ラ ー ゼ N (pectase of the フ ー ズ of Mitsubishi Chemical corporate system) 2g as enzyme; In addition carry out 1 identical operation, obtain Bx35 ° green-tea extract (the present invention's article 9) 263.8g (pH5.3, with respect to green tea yield 131.8%) with embodiment.

Embodiment 10

(enzyme of embodiment 1 only being replaced to the example of cellulase)

In embodiment 1, use cellulase T " ア マ ノ " 4 (cellulase of day wild エ Application ザ イ system corporate system) 2g as enzyme; In addition carry out 1 identical operation, obtain Bx35 ° green-tea extract (the present invention's article 10) 268.1g (pH5.3, with respect to green tea yield 134.1%) with embodiment.

Embodiment 11

(enzyme of embodiment 1 only being replaced to the example of dextranase)

In embodiment 1, use Star ニ カ ー ゼ FN (big with change into) 2g as enzyme; In addition carry out 1 identical operation, obtain Bx35 ° green-tea extract (the present invention's article 11) 264.0g (pH5.3, with respect to green tea yield 132.0%) with embodiment.

Embodiment 12

(enzyme of embodiment 1 only being replaced to the example of mannase)

In embodiment 1, use セ Le ロ シ Application GM5 (エ イ チ PVC イ ア イ) 2g as enzyme; In addition carry out 1 identical operation, obtain Bx35 ° green-tea extract (the present invention's article 12) 259.0g (pH5.3, with respect to green tea yield 129.5%) with embodiment.

Embodiment 13

(enzyme of embodiment 1 only being replaced to the example of alpha-galactosidase)

In embodiment 1, use Sumizyme AGS (new Japanese chemical industry) 2g as enzyme; In addition carry out 1 identical operation, obtain Bx35 ° green-tea extract (the present invention's article 13) 263.2g (pH5.3, with respect to green tea yield 131.6%) with embodiment.

Embodiment 14

(enzyme of embodiment 1 only being replaced to the example of invertase)

In embodiment 1, use Sumizyme INV (new Japanese chemical industry) 2g as enzyme; In addition carry out 1 identical operation, obtain Bx35 ° green-tea extract (the present invention's article 14) 252.6g (pH5.3, with respect to green tea yield 126.3%) with embodiment.

Embodiment 15

(enzyme of embodiment 1 being replaced to the example of cellulase and hemicellulase)

In embodiment 1, use cellulase T " ア マ ノ " 4 (cellulase of day wild エ Application ザ イ system corporate system) 2g and Sumizyme X (hemicellulase of new Japanese chemical industrial company system) 2g as enzyme; In addition carry out 1 identical operation, obtain Bx35 ° green-tea extract (the present invention's article 15) 324.3g (pH5.3, with respect to green tea yield 162.2%) with embodiment.

Embodiment 16

(enzyme of embodiment 1 being replaced to the example of cellulase and mannase)

In embodiment 1, use cellulase T " ア マ ノ " 4 (cellulase of day wild エ Application ザ イ system corporate system) 2g and セ Le ロ シ Application GM5 (エ イ チ PVC イ ア イ) 2g as enzyme; In addition carry out 1 identical operation, obtain Bx35 ° green-tea extract (the present invention's article 16) 327.4g (pH5.3, with respect to green tea yield 163.7%) with embodiment.

Embodiment 17

(enzyme of embodiment 1 being replaced to the example of cellulase and alpha-galactosidase)

In embodiment 1, use cellulase T " ア マ ノ " 4 (cellulase of day wild エ Application ザ イ system corporate system) 2g and Sumizyme AGS (new Japanese chemical industry) 2g as enzyme; In addition carry out 1 identical operation, obtain Bx35 ° green-tea extract (the present invention's article 17) 325.3g (pH5.3, with respect to green tea yield 162.7%) with embodiment.

Embodiment 18

(enzyme of embodiment 1 is replaced to cellulase and examples of proteases)

In embodiment 1, use cellulase T " ア マ ノ " 4 (cellulase of day wild エ Application ザ イ system corporate system) 2g and protease M (ア マ ノ エ Application ザ イ system (strain)) 2g as enzyme; In addition carry out 1 identical operation, obtain Bx35 ° green-tea extract (the present invention's article 18) 321.4g (pH5.3, with respect to green tea yield 160.7%) with embodiment.

Embodiment 19

(enzyme of embodiment 1 being replaced to the example of hemicellulase and dextranase)

In embodiment 1, use Sumizyme X (hemicellulase of new Japanese chemical industrial company system) 2g and Star ニ カ ー ゼ FN (big and change into) 2g as enzyme; In addition carry out 1 identical operation, obtain Bx35 ° green-tea extract (the present invention's article 19) 322.9g (pH5.3, with respect to green tea yield 161.5%) with embodiment.

Embodiment 20

(enzyme of embodiment 1 being replaced to the example of glucoamylase, hemicellulase and invertase)

In embodiment 1, use Sumizyme (glucose-amylase activity 2,000U/g as enzyme; The glucoamylase of new Japanese chemical industrial company system) 2g, Sumizyme X (hemicellulase of new Japanese chemical industrial company system) 2g and Sumizyme INV (new Japanese chemical industry) 2g; In addition carry out 1 identical operation, obtain Bx35 ° green-tea extract (the present invention's article 20) 328.1g (pH5.3, with respect to green tea yield 164.1%) with embodiment.

Embodiment 21

(enzyme of embodiment 1 is replaced to glucoamylase, cellulase and examples of proteases)

In embodiment 1, use Sumizyme (glucose-amylase activity 2,000U/g as enzyme; The glucoamylase of new Japanese chemical industrial company system) 2g, cellulase T " ア マ ノ " 4 (cellulase of day wild エ Application ザ イ system corporate system) 2g and protease M (5,500U/g; ア マ ノ エ Application ザ イ system (strain)) in addition 2g carries out the 1 identical operation with embodiment, obtains Bx35 ° green-tea extract (the present invention's article 21) 324.5g (pH5.3, with respect to green tea yield 162.3%).

The sensory evaluation of extract

Use respectively ion exchange water with the present invention's article 1～21 and relatively article 1～3 be diluted to 50 times; By 10 well-trained reviewers sensory evaluation is carried out in natural sense, fresh feel, sweet taste, flavour, assorted flavor, happy sense, very good: 10 minutes, good: 8 minutes, good slightly: 6 minutes, poor slightly: 4 minutes, poor: 2 minutes, non-constant: 0 minute.

The kind and the sensory evaluation of enzyme are shown in below table 1.

[table 1]

As shown in table 1, the reviewer is evaluated as, and reclaims all untapped relatively article 1 of perfume and enzyme processing and almost experiences less than natural sense, fresh feel, and being difficult to a little recognize is tea, and sweet taste, flavour are also few in addition, and assorted flavor, happy sense also lack.And the evaluation of the present invention's article aspect all of natural sense, fresh feel, sweet taste, flavour, assorted flavor, happy sense is all high.

Wherein, the flavour of the present invention's article 1 of handling through protease and tannase is strong, and sweet taste is also strong in addition, and assorted flavor less, happy sense is good.

All strong, the assorted flavor of sweet taste, flavour of the present invention's article 2 of handling through glucoamylase and hemicellulase in addition,, the present invention's article 3 of handling through glucoamylase and pectase and the present invention's article 4 of crossing through glucoamylase and cellulose treatment less, happy sense is good.

Relatively article 2 be do not use reclaim fragrant, directly carried out the extract that protease and tannase are handled; For sweet taste, flavour, assorted flavor, happy sense, more relatively article 1 make moderate progress, but not quite can experience nature sense, fresh feel; Be difficult to recognize to be the tea of which kind of kind a little; This is part reviewer's evaluation, compares with the present invention's article 1～4, thinks poorly of.

Relatively article 3 have been to use and have reclaimed fragrant but do not carry out the extract that enzyme is handled, naturally sense, fresh feel more relatively article 1 make moderate progress, but sweet taste, few, the assorted flavor of flavour, happy sense shortage compare with the present invention's article 1～4, estimates far short of what is expectedly.

On the other hand; Sweet taste, flavour, assorted flavor, the happy sense of the present invention's article 7～13 of glucoamylase, hemicellulase, pectase, cellulase, dextranase, mannase, the independent effect of alpha-galactosidase are compared with the relatively article 3 that do not use enzyme fully, made moderate progress.In addition, sweet taste, flavour, assorted flavor, the happy sense of the present invention's article 5, the present invention's article 6 and the present invention's article 14 that, protease independent with tannase is crossed with the invertase individual processing are separately compared with the relatively article 3 that do not use enzyme fully, all how much make moderate progress.

In addition, each 2 kinds of enzyme of the plain enzyme of conjugate fiber and mannase, cellulase and alpha-galactosidase, cellulase and protease have carried out local flavor and the present invention's article 7～13rd of the present invention's article 16～18 of processing, equal extent.

And each 2 kinds of enzyme of the plain enzyme of conjugate fiber and hemicellulase, hemicellulase and dextranase have carried out the present invention's article 15 that enzyme handles and have compared with the present invention's article 7～13 with the present invention's article 19, sweet taste, flavour, assortedly distinguish the flavor of, happy sense is good slightly.

In addition, except glucoamylase and hemicellulase, also be added with invertase, promptly, further added the present invention's article 20 of invertase with respect to the present invention's article 2 and compared with the present invention's article 2, sweet taste, flavour, assorted flavor, happy sense are poor slightly.

And, except glucoamylase and cellulase, also be added with protease, promptly, further added the present invention's article 21 of protease with respect to the present invention's article 4 and compared with the present invention's article 4, sweet taste, flavour, assorted flavor, happy sense are poor slightly.

Reference example 1

The affirmation of invertase activity

The present invention's article 20 and the present invention's article 21 are with respect to the present invention's article 2 and the present invention's article 4 respectively and used the product of other enzymes, and in sensory evaluation, sweet taste, flavour, assorted flavor, happy sense are all poor slightly than the present invention's article 2 and the present invention's article 4.In addition, when being invertase separately, almost do not observe the improvement effect of sweet taste, flavour, assorted flavor, happy sense in the table 1.Therefore, think that invertase activity may cause some negative effect, therefore have or not invertase activity to measure the enzyme that uses in the foregoing description.

The assay method that has or not invertase activity:

Lyase 0.005g in 0.5% aqueous solution 100ml of sucrose 40 ℃ of held 1 round the clock, uses commercially available glucose test paper (ウ リ エ ー ス (registration mark) Ga (テ Le モ strain formula meeting corporate system), judgement;-: less than 50mg/100ml, ±: about 50mg/100ml ,+: about 100mg/100ml, ++: about 500mg/100ml, +++: about 2000mg/100ml) judge.The result is shown in below table 2.

[table 2]

The invertase activity of employed enzyme

In the mensuration under these conditions, do not see invertase activity among cellulase T " ア マ ノ " 4, Sumizyme X, Sumizyme and the Star ニ カ ー ゼ FN, visible invertase activity in the enzyme in addition.

Therefore, can know in the table 1 that the saccharic catabolic enzyme of combination more than 2 kinds carried out in the present invention's article 2～4 and 15～21 of enzyme processing, estimating the enzyme that uses in good especially the present invention's article 2～4 all is the combinations that do not contain invertase activity.

Be added with the sensory evaluation of the green tea beverage of teas extract

In being heated to 80 ℃ ion exchange water 20kg, drop into the Shizuoka county and produce green tea 1kg; Slowly stir after 5 minutes, tealeaves is separated, with the liquid cools to 20 after the separation ℃ with 40 order woven wires; Obtain extract 14kg; Add sodium ascorbate 7.0g (500ppm), use No.2 filter paper (ADVANTEC corporate system: hold back particle diameter 5 μ) to filter, obtain the green tea beverage stoste (assay value of green tea beverage stoste; Bx:2.22 °, pH:6.4, tannic acid content (tartaric acid iron processes): amino acid content 0.44%: 0.071%).It is divided into aliquot, is diluted to 10 times (mass ratioes) with ion exchange water, in its dilution, add respectively the present invention's article 1～21 of 0.3% and relatively article 1～3 modulate; At 137 ℃ of following heat sterilizations after 30 seconds; Be cooled to 88 ℃, be filled in the 500ml plastic bottle, keep after 2 minutes; Be cooled to room temperature (25 ℃), process the green tea beverage of plastic bottle.It is contrast that each green tea beverage does not have the article of interpolation with the teas extract, is estimated by 10 reviewers.Evaluation criterion is following: do not have to add article be under 5 minutes the situation making, for natural sense, fresh feel, sweet taste, flavour, assorted flavor, happy sense, very good: 10 minutes, well: 8 minutes, lack: 6 minutes, poor slightly: 4 minutes, poor: 2 minutes, non-constant 0 minute.

The kind and the sensory evaluation of enzyme are shown in below table 3.

[table 3]

As shown in table 3, reviewer's evaluation is: the sweet taste, flavour, happy sense, the assorted flavor that have added the green tea beverage that reclaims fragrant and all untapped relatively article 1 of enzyme processing make moderate progress, but natural sense, fresh feel are not almost played effect.

In addition; Added to use and reclaimed fragrant but carried out the natural sense, fresh feel of the green tea beverage of the relatively article 2 that protease and tannase handle and interpolation article not much at one; Though sweet taste, flavour, assorted flavor, happy sense more relatively article 1 make moderate progress, its evaluation is still poor.

In addition, particularly be aspect the flavor evaluation inequality of flavour, assorted flavor, happy sense etc. though added the green tea beverage that reclaims the fragrant relatively article 3 that do not use enzyme fully.

With respect to having added these relatively green tea beverages of article, the evaluation of having added the green tea beverage of the present invention's article all is: natural sense, fresh feel, sweet taste, flavour, happy sense increase, and assorted flavor reduces, excellent flavor.

In the present invention's article, the flavour of green tea beverage that has added the present invention's article 1 of handling through protease and tannase is strong especially, and also strong, the assorted flavor of sweet taste less in addition, happy sense is good.

In addition, added the green tea beverage of the present invention's article 2 of handling through glucoamylase and hemicellulase, the green tea beverage that added the green tea beverage of the present invention's article 3 of handling through glucoamylase and pectase and added the present invention's article 4 of crossing through glucoamylase and cellulose treatment all be very strong, the assorted flavor of sweet taste, flavour less, happy sense is good.

On the other hand; Added the green tea beverage of the present invention's article 7～13 that make glucoamylase, hemicellulase, pectase, cellulase, dextranase, mannase, the independent effect of alpha-galactosidase respectively and compared with the green tea beverage that has added the relatively article 3 that do not use enzyme, how many sweet taste, flavour, assorted flavor, happy senses make moderate progress.In addition; Added the green tea beverage of the present invention's article 5, the present invention's article 6 and the present invention's article 14 that, protease independent with tannase crosses with the invertase individual processing separately and compared with the green tea beverage that has added the relatively article 3 that do not use enzyme fully, sweet taste, flavour, assorted flavor, happy sense all have improvement slightly.

For other enzyme item for disposal, the evaluation during with direct dilution teas extract is basic identical.

Embodiment 22

(utilizing gas-liquid counter current contact extraction method that jasmine tea is carried out the example that fragrance reclaims, bottoms carried out protease and tannase processing)

Using hammer-mill (screen cloth 1.2mm) that Fujian Province is produced jasmine tea 40kg pulverizes; Add the water 360kg that is dissolved with sodium ascorbate 0.24kg; Process slurry form, utilize gas-liquid counter current contact extraction method obtaining to reclaim fragrant 16kg (with respect to jasmine tea 40%) (below be called reclaim fragrant (c)) under the following condition.

A device: SCC Model1000! フ レ ー バ ー テ ッ Network corporate system)

Treatment conditions

Raw material supplying speed: 700L/Hr

Vapor flow rate: 55kg/hr

Bar tape speed: about 4%

Column bottom temperature: 100 ℃

Tower upper temp: 100 ℃

Vacuum: atmospheric pressure.

Residue slurry 400kg (being equivalent to jasmine tea 40kg) (below be called residue slurry (d)) is cooled to 45 ℃; Gather 2kg (being equivalent to jasmine tea 200g) wherein equably, reach 90 ℃ of temperature sterilizations after, be cooled to 40 ℃ immediately; Interpolation protease M (5,500U/g; ア マ ノ エ Application ザ イ system (strain)) 2g and tannase (500U/g; キ ッ コ ー マ Application (strain)) 2g stirred 30 minutes under uniform temp, and after fully enzyme being mixed, standing and reacting is 16 hours under uniform temp.Through after the reaction time, add sodium ascorbate 0.5g and mix fully, carry out Separation of Solid and Liquid afterwards; Heating made enzyme deactivation in 1 minute under 90 ℃; After being cooled to 20 ℃, use is coated with diatomaceous suction filter in advance and filters, and obtains the filtrating 1724g (Bx7.1 °, pH4.6) of clarification.Use Rotary Evaporators that filtrate decompression is concentrated, obtain Bx50.0 ° concentrate 244.8g.Then; In concentrate, add to reclaim fragrant (c) 81.6g (the Bx conversion solid constituent of concentrate 2/3 amount) and sodium ascorbate 0.49g (the Bx conversion solid constituent of concentrate 0.4% amount); Fully mix the back and add water 22.8g, Bx is adjusted to 35 °.Then,, be cooled to 30 ℃, be filled in the container, obtain Bx35 ° jasmine tea extract (the present invention's article 22) 349.7g (pH5.3, with respect to jasmine tea yield 174.9%) 90 ℃ of down heating 1 minute.

Embodiment 23

(enzyme of embodiment 22 being replaced to the example of glucoamylase and hemicellulase)

In embodiment 22, use Sumizyme (glucose-amylase activity 2,000U/g as enzyme; The glucoamylase of new Japanese chemical industrial company system) 2g and Sumizyme X (hemicellulase of new Japanese chemical industrial company system) 2g; In addition carry out 22 identical operations, obtain Bx35 ° jasmine tea extract (the present invention's article 23) 342.3g (pH5.3, with respect to jasmine tea yield 171.1%) with embodiment.

Embodiment 24

(enzyme of embodiment 22 being replaced to the example of glucoamylase and pectase)

In embodiment 22, use Sumizyme (glucose-amylase activity 2,000U/g as enzyme; The glucoamylase of new Japanese chemical industrial company system) 2g and ス Network ラ ー ゼ N (pectase of the Off ー ズ of Mitsubishi Chemical corporate system) 2g; In addition carry out 22 identical operations, obtain Bx35 ° jasmine tea extract (the present invention's article 24) 338.9g (pH5.3, with respect to jasmine tea yield 169.5%) with embodiment.

Embodiment 25

(enzyme of embodiment 22 being replaced to the example of glucoamylase and cellulase)

In embodiment 22, use Sumizyme (glucose-amylase activity 2,000U/g as enzyme; The glucoamylase of new Japanese chemical industrial company system) 2g and cellulase T " ア マ ノ " (cellulase of day wild エ Application ザ イ system corporate system) 2g; In addition carry out 22 identical operations, obtain Bx35 ° jasmine tea extract (the present invention's article 25) 342.6g (pH5.3, with respect to jasmine tea yield 171.3%) with embodiment.

Comparative example 4

(jasmine tea is not carried out fragrance and reclaims, also do not carry out the example that enzyme is handled)

Use hammer-mill (screen cloth 1.2mm) to pulverize, add the water 3600g that is dissolved with sodium ascorbate 2.4g, process slurry form with used identical jasmine tea 200g among the embodiment 22; Be heated to 90 ℃ and extract after 10 minutes, be cooled to 30 ℃ immediately, carry out Separation of Solid and Liquid; 90 ℃ of following heat sterilizations 1 minute; After being cooled to 20 ℃, use is coated with diatomaceous suction filter in advance and filters, and obtains the filtrating 1685g (Bx4.1 °, pH5.2) of clarification.Use Rotary Evaporators that filtrate decompression is concentrated, obtain Bx35 ° concentrate 197.2g.Then,, be cooled to 30 ℃, be filled in the container, obtain Bx35 ° jasmine tea extract (relatively article 4) 197.2g (pH5.3, with respect to jasmine tea yield 98.6%) 90 ℃ of down heating 1 minute.

Comparative example 5

(jasmine tea not being carried out the example that fragrance reclaimed, carried out protease and tannase processing)

Use hammer-mill (screen cloth 1.2mm) that Fujian Province is produced jasmine tea 200g and pulverize, add the water 3600g that is dissolved with sodium ascorbate 2.4g, process slurry form, 90 ℃ down after the sterilization, be cooled to 40 ℃ immediately, add protease M (5,500U/g; ア マ ノ エ Application ザ イ system (strain)) 2g and tannase (500U/g; キ ッ コ ー マ Application (strain)) 2g stirred 30 minutes under uniform temp, and after fully enzyme being mixed, standing and reacting is 16 hours under uniform temp.Through after the reaction time, add sodium ascorbate 0.5g and mix fully, carry out Separation of Solid and Liquid afterwards; Heating made enzyme deactivation in 1 minute under 90 ℃; After being cooled to 20 ℃, use is coated with diatomaceous suction filter in advance and filters, and obtains the filtrating 1721g (Bx7.2 °, pH4.6) of clarification.Use Rotary Evaporators that filtrate decompression is concentrated, obtain Bx35 ° concentrate 325.9g.Then,, be cooled to 30 ℃, be filled in the container, obtain Bx35 ° jasmine tea extract (relatively article 5) 325.9g (pH5.3, with respect to jasmine tea yield 163.0%) 90 ℃ of down heating 1 minute.

Comparative example 6

(utilizing gas-liquid counter current contact extraction method that jasmine tea is carried out fragrance reclaims, bottoms is not carried out the example that the direct enforcement of enzyme processing is extracted)

Gather residue slurry (d) 2kg (being equivalent to jasmine tea 200g) that embodiment 22 obtains equably, reach 90 ℃ of temperature sterilizations after, be cooled to 40 ℃ immediately; Adding sodium ascorbate 0.5g mixes fully; Carry out Separation of Solid and Liquid afterwards, 90 ℃ of down heating 1 minute, be cooled to 20 ℃ after; Use is coated with diatomaceous suction filter in advance and filters, and obtains the filtrating 1679g (Bx4.0 °, pH5.2) of clarification.Use Rotary Evaporators that filtrate decompression is concentrated, obtain Bx50.0 ° concentrate 134.3g.Then, in concentrate, add to reclaim fragrant (c) 44.8g (the Bx conversion solid constituent of concentrate 2/3 amount) and sodium ascorbate 0.27g (the Bx conversion solid constituent of concentrate 0.4% amount), fully mix back interpolation water, Bx is adjusted to 35 °.Then,, be cooled to 30 ℃, be filled in the container, obtain Bx35 ° jasmine tea extract (relatively article 6) 191.4g (pH5.5, with respect to jasmine tea yield 95.7%) 90 ℃ of down heating 1 minute.

The sensory evaluation of teas extract

Use respectively ion exchange water with the present invention's article 22～25 and relatively article 4～6 be diluted to 50 times; By 10 well-trained reviewers sensory evaluation is carried out in natural sense, fresh feel, sweet taste, flavour, assorted flavor, happy sense, very good: 10 minutes, good: 8 minutes, good slightly: 6 minutes, poor slightly: 4 minutes, poor: 2 minutes, non-constant: 0 minute.

The kind and the sensory evaluation of enzyme are shown in below table 4.

[table 4]

As shown in table 4, jasmine tea almost is identical result with green tea.Be being evaluated as of reviewer; Reclaiming all untapped relatively article 4 of perfume and enzyme processing almost experiences less than natural sense, fresh feel; Sweet taste, flavour are also few; Assorted flavor, happy sense also lack, and the present invention's article in natural sense, fresh feel, sweet taste, flavour, assortedly distinguish the flavor of, happy sense all aspect evaluation all high.

Wherein, the flavour of the present invention's article 22 of handling through protease and tannase is strong especially, and sweet taste is also strong in addition, and assorted flavor less, happy sense is good.

All very strong, the assorted flavor of sweet taste, flavour of the present invention's article 23 of handling through glucoamylase and hemicellulase in addition,, the present invention's article 24 of handling through glucoamylase and pectase and the present invention's article 25 of crossing through glucoamylase and cellulose treatment less, happy sense is good.

Relatively article 5 be do not use reclaim fragrant, directly carried out the extract that protease and tannase are handled; For sweet taste, flavour, assorted flavor, happy sense, more relatively article 4 make moderate progress, but experience basically less than natural sense, fresh feel; Very difficult understanding is the tea of which kind of kind; This is part reviewer's evaluation, compares with the present invention's article 22～25, thinks poorly of.

Relatively article 6 have been to use and have reclaimed fragrant but do not carry out the teas extract that enzyme is handled, naturally sense, fresh feel more relatively article 4 make moderate progress, but sweet taste, few, the assorted flavor of flavour, happy sense shortage compare with the present invention's article 22～25, estimates far short of what is expectedly.

Be added with the sensory evaluation of the jasmine tea beverage of teas extract

In being heated to 80 ℃ ion exchange water 20kg, drop into Fujian Province and produce Jasmine tealeaves 1kg, slowly stir 5 minutes after, with 40 order woven wires tealeaves is separated, with the liquid cools to 20 after separating ℃, acquisition extract 14kg.In the gained extract, add sodium ascorbate 7.0g (500ppm), use No.2 filter paper (ADVANTEC corporate system: hold back particle diameter 5 μ) to filter, obtain the jasmine tea beverage stoste (assay value of jasmine tea beverage stoste; Bx:2.43 °, pH:6.3, tannic acid content (tartaric acid iron processes): amino acid content 0.38%: 0.055%).It is divided into aliquot, is diluted to 10 times (mass ratioes) with ion exchange water, in its dilution, add respectively the present invention's article 22～25 of 0.3% and relatively article 4～6 modulate; At 137 ℃ of following heat sterilizations after 30 seconds; Be cooled to 88 ℃, be filled in the 500ml plastic bottle, keep after 2 minutes; Be cooled to room temperature (25 ℃), process the jasmine tea beverage of plastic bottle.It is contrast that each jasmine tea beverage does not have the article of interpolation with the teas extract, is estimated by 10 reviewers.Evaluation criterion is following: do not have to add article be under 5 minutes the situation making, for natural sense, fresh feel, sweet taste, flavour, assorted flavor, happy sense, very good: 10 minutes, well: 8 minutes, lack: 6 minutes, poor slightly: 4 minutes, poor: 2 minutes, non-constant 0 minute.

The kind and the sensory evaluation of enzyme are shown in below table 5.

[table 5]

As shown in table 5, the result of jasmine tea beverage and green tea beverage much at one.That is, reviewer's evaluation is: the sweet taste, flavour, happy sense, the assorted flavor that have added the jasmine tea beverage that reclaims fragrant and all untapped relatively article 4 of enzyme processing make moderate progress, but natural sense, fresh feel are not almost played effect.

In addition; Added to use and reclaimed fragrant but carried out the natural sense, fresh feel of the jasmine tea beverage of the relatively article 5 that protease and tannase handle and interpolation article not much at one; Compare and make moderate progress though the jasmine tea beverage of article 4 has been added relatively in sweet taste, flavour, assorted flavor, happy sense, its evaluation is still poor.

In addition, particularly be aspect the flavor evaluation inequality of flavour, assorted flavor, happy sense etc. though added the jasmine tea beverage that reclaims the fragrant relatively article 6 that do not use enzyme fully.

With respect to having added these relatively jasmine tea beverages of article, the evaluation of having added the jasmine tea beverage of the present invention's article is: natural sense, fresh feel, sweet taste, flavour, happy sense all increase, and assorted flavor reduces, excellent flavor.

The flavour of jasmine tea beverage that has added the present invention's article 22 of handling through protease and tannase is strong especially, and also strong, the assorted flavor of sweet taste less in addition, happy sense is good.

In addition, added the jasmine tea beverage of the present invention's article 23 of handling through glucoamylase and hemicellulase, all very strong, the assorted flavor of sweet taste, flavour that added the jasmine tea beverage of the present invention's article 24 of handling through glucoamylase and pectase and added the jasmine tea beverage of the present invention's article 25 of crossing through glucoamylase and cellulose treatment less, happy sense is good.

Embodiment 26

(utilizing the tower steam distillation that oolong tea is carried out the example that fragrance reclaims, bottoms carried out protease and tannase processing)

Use hammer-mill (screen cloth 1.2mm) to pulverize as the Iron Guanyin (K-103) of oolong tea; 1kg wherein is filled in 3 liters of towers; Behind nitrogen replacement in the system, under atmospheric pressure send into water vapour by tower bottom, carry out steam distillation; Use cooling tube to make the water vapour that contains fragrance that obtains by tower top condensing, obtain fragrance distillate 400g (with respect to oolong tea 40%, below be called reclaim fragrant (e)).Gained fragrance distillate is carried out being cooled to about 4 ℃ after the nitrogen-sealed, and sealing is preserved.After moving to the residue in the tower in the stirred tank, pack into and dissolved the water 9kg of sodium ascorbate 6g, after stirring 30 minutes under 40 ℃, interpolation protease M (5,500U/g; ア マ ノ エ Application ザ イ system (strain)) 10g and tannase (500U/g; キ ッ コ ー マ Application (strain)) 10g stirred 30 minutes down synthermal, after enzyme is mixed fully, synthermal standing and reacting down 16 hours.Through after the reaction time, add sodium ascorbate 2.5g and mix fully, carry out Separation of Solid and Liquid; Heating made enzyme deactivation in 1 minute under 90 ℃; After being cooled to 20 ℃, use is coated with diatomaceous suction filter in advance and filters, and obtains the filtrating 8029g (Bx7.6 °, pH4.7) of clarification.Use Rotary Evaporators that filtrate decompression is concentrated, obtain Bx50.0 ° concentrate 1097.4g.Then, in concentrate, add to reclaim fragrant (e) 365.8g (the Bx conversion solid constituent of concentrate 2/3 amount) and sodium ascorbate 2.2g (the Bx conversion solid constituent of concentrate 0.4% amount), fully mix back interpolation water, Bx is adjusted to 35 °.Then,, be cooled to 30 ℃, be filled in the container, obtain Bx35 ° oolong tea extract (the present invention's article 26) 1567g (pH5.3, with respect to oolong tea yield 156.7%) 90 ℃ of down heating 1 minute.

Embodiment 27

(enzyme of embodiment 26 being replaced to the example of glucoamylase and hemicellulase)

In embodiment 26, use Sumizyme (glucose-amylase activity 2,000U/g as enzyme; The glucoamylase of new Japanese chemical industrial company system) 10g and Sumizyme X (hemicellulase of new Japanese chemical industrial company system) 10g; In addition carry out 26 identical operations, obtain Bx35 ° oolong tea extract (the present invention's article 27) 987.6g (pH5.3, with respect to oolong tea yield 98.8%) with embodiment.

Embodiment 28

(enzyme of embodiment 26 being replaced to the example of glucoamylase and pectase)

In embodiment 26, use Sumizyme (glucose-amylase activity 2,000U/g as enzyme; The glucoamylase of new Japanese chemical industrial company system) 10g and ス Network ラ ー ゼ N (pectase of the Off ー ズ of Mitsubishi Chemical corporate system) 10g; In addition carry out 26 identical operations, obtain Bx35 ° oolong tea extract (the present invention's article 28) 977.6g (pH5.3, with respect to oolong tea yield 97.8%) with embodiment.

Embodiment 29

(enzyme of embodiment 26 being replaced to the example of glucoamylase and cellulase)

In embodiment 26, use Sumizyme (glucose-amylase activity 2,000U/g as enzyme; The glucoamylase of new Japanese chemical industrial company system) 10g and cellulase T " ア マ ノ " (cellulase of day wild エ Application ザ イ system corporate system) 10g; In addition carry out 26 identical operations, obtain Bx35 ° green-tea extract (the present invention's article 29) 985.3g (pH5.3, with respect to oolong tea yield 98.5%) with embodiment.

Comparative example 7

(oolong tea is not carried out fragrance and reclaims, also do not carry out the example that enzyme is handled)

Will with embodiment 26 in use the identical olong tea powder 1kg that minces to pack among the water 9kg that is dissolved with sodium ascorbate 6g; Be heated to 90 ℃ and extract after 10 minutes, be cooled to 40 ℃ immediately, carry out Separation of Solid and Liquid; 90 ℃ of following heat sterilizations 1 minute; After being cooled to 20 ℃, use is coated with diatomaceous suction filter in advance and filters, and obtains the filtrating 7835g (Bx3.8 °, pH5.2) of clarification.Use Rotary Evaporators that filtrate decompression is concentrated, obtain Bx35 ° concentrate 850.7g.Then, heated 1 minute down, be cooled to 30 ℃, be filled in the container, obtain Bx35 ° oolong tea extract (relatively article 7) 850.7g (pH5.3, oolong tea yield 85.1%) at 90 ℃.

Comparative example 8

(oolong tea not being carried out the example that fragrance reclaimed, carried out protease and tannase processing)

Will with embodiment 26 in use the identical olong tea powder 1kg that minces to pack among the water 9kg that is dissolved with sodium ascorbate 6g, be heated to 90 ℃ and extract after 10 minutes, be cooled to 40 ℃ immediately, add protease M (5,500U/g; ア マ ノ エ Application ザ イ system (strain)) 10g and tannase (500U/g; キ ッ コ ー マ Application (strain)) 10g stirred 30 minutes down synthermal, after enzyme is mixed fully, synthermal standing and reacting down 16 hours.Through after the reaction time, add sodium ascorbate 2.5g and mix fully, carry out Separation of Solid and Liquid; Heating made enzyme deactivation in 1 minute under 90 ℃; After being cooled to 20 ℃, use is coated with diatomaceous suction filter in advance and filters, and obtains the filtrating 7983g (Bx7.6 °, pH4.7) of clarification.Use Rotary Evaporators that filtrate decompression is concentrated, obtain Bx35.0 ° concentrate 1733.4g.Then,, be cooled to 30 ℃, be filled in the container, obtain Bx35 ° oolong tea extract (relatively article 8) 1733.4g (pH5.3, with respect to oolong tea yield 177.3%) 90 ℃ of down heating 1 minute.

Comparative example 9

(utilizing the tower steam distillation that oolong tea is carried out fragrance reclaims, bottoms is not carried out the example that enzyme is handled direct enforcement extraction)

Will with embodiment 26 in use the identical olong tea powder 1kg that minces to be filled in 3 liters of towers; Behind nitrogen replacement in the system; Under atmospheric pressure send into water vapour by tower bottom; Carry out steam distillation, use cooling tube to make the water vapour that contains fragrance that obtains by tower top condensing, obtain fragrance distillate 400g (with respect to oolong tea 40%, below be called reclaim fragrant (e)).Gained fragrance distillate is carried out being cooled to about 4 ℃ after the nitrogen-sealed, and sealing is preserved.After moving to the residue in the tower in the stirred tank, pack into and dissolved the water 9kg of sodium ascorbate 6g, be heated to 90 ℃ and extract after 10 minutes; Be cooled to 40 ℃ immediately; Carry out Separation of Solid and Liquid,, be cooled to 20 ℃ 90 ℃ of following heat sterilizations 1 minute; Use afterwards is coated with diatomaceous suction filter in advance and filters, and obtains the filtrating 7923g (Bx3.8 °, pH5.2) of clarification.Use Rotary Evaporators that filtrate decompression is concentrated, obtain Bx50.0 ° concentrate 602.1g.Then, in concentrate, add to reclaim fragrant (e) 200.7g (the Bx conversion solid constituent of concentrate 2/3 amount) and sodium ascorbate 1.2g (the Bx conversion solid constituent of concentrate 0.4% amount), fully mix back interpolation water, Bx is adjusted to 35 °.Then,, be cooled to 30 ℃, be filled in the container, obtain Bx35 ° oolong tea extract (relatively article 9) 860.1g (pH5.3, with respect to oolong tea yield 86.0%) 90 ℃ of down heating 1 minute.

The sensory evaluation of teas extract

Use respectively ion exchange water with the present invention's article 26～29 and relatively article 7～9 be diluted to 50 times; By 10 well-trained reviewers sensory evaluation is carried out in natural sense, fresh feel, sweet taste, flavour, assorted flavor, happy sense, very good: 10 minutes, good: 8 minutes, good slightly: 6 minutes, poor slightly: 4 minutes, poor: 2 minutes, non-constant: 0 minute.

The kind and the sensory evaluation of enzyme are shown in below table 6.

[table 6]

As shown in table 6, the oolong tea that utilizes the tower steam distillation to reclaim fragrance is result much at one with aforementioned green tea and oolong tea also.Be being evaluated as of reviewer: reclaim all untapped relatively article 7 of perfume and enzyme processing and almost experience less than natural sense, fresh feel; Sweet taste, flavour are also few; Assorted flavor, happy sense also lack, and the present invention's article in natural sense, fresh feel, sweet taste, flavour, assortedly distinguish the flavor of, happy sense all aspect evaluation all high.

The flavour of the present invention's article 26 of handling through protease and tannase is strong especially, and sweet taste is also strong in addition, and assorted flavor less, happy sense is good.

All very strong, the assorted flavor of sweet taste, flavour of the present invention's article 27 of handling through glucoamylase and hemicellulase in addition,, the present invention's article 28 of handling through glucoamylase and pectase and the present invention's article 29 of crossing through glucoamylase and cellulose treatment less, happy sense is good.

Relatively article 8 are not use to reclaim teas extract fragrant but that carried out protease and tannase processing; Sweet taste, few, the assorted flavor of flavour, happy sense more relatively article 7 make moderate progress; But almost experience less than natural sense, fresh feel, being difficult to recognize is the tea of which kind of kind, and this is part reviewer's evaluation; Compare with the present invention's article 26～29, think poorly of.

Relatively article 9 have been to use and have reclaimed fragrant but do not carry out the teas extract that enzyme is handled, naturally sense, fresh feel more relatively article 7 make moderate progress, but sweet taste, few, the assorted flavor of flavour, happy sense shortage compare with the present invention's article 26～29, estimates far short of what is expectedly.

Be added with the sensory evaluation of the oolong tea beverage of teas extract

In being heated to 80 ℃ ion exchange water 20kg, drop into oolong (look kind S-103) 1kg, slowly stir 5 minutes after, with 40 order woven wires tealeaves is separated, with the liquid cools to 20 after separating ℃, obtain extract 14kg.In the gained extract, add sodium ascorbate 7.0g (500ppm), use No.2 filter paper (ADVANTEC corporate system: hold back particle diameter 5 μ) to filter, obtain the oolong tea beverage stoste (assay value of oolong tea stoste; Bx:2.43 °, pH:6.2, tannic acid content (tartaric acid iron processes): amino acid content 0.41%: 0.031%).It is divided into aliquot, is diluted to 10 times (mass ratioes) with ion exchange water, in its dilution, add respectively the present invention's article 26～29 of 0.3% and relatively article 7～9 modulate; At 137 ℃ of following heat sterilizations after 30 seconds; Be cooled to 88 ℃, be filled in the 500ml plastic bottle, keep after 2 minutes; Be cooled to room temperature (25 ℃), process the oolong tea beverage of plastic bottle.It is contrast that each oolong tea beverage does not have the article of interpolation with extract, is estimated by 10 reviewers.Evaluation criterion is following: do not have to add article be under 5 minutes the situation making, for natural sense, fresh feel, sweet taste, flavour, assorted flavor, happy sense, very good: 10 minutes, well: 8 minutes, lack: 6 minutes, poor slightly: 4 minutes, poor: 2 minutes, non-constant 0 minute.

The kind and the sensory evaluation of enzyme are shown in below table 7.

[table 7]

As shown in table 7, oolong tea beverage also with the result of green tea beverage and jasmine tea beverage much at one.That is, reviewer's evaluation is: the sweet taste, flavour, happy sense, the assorted flavor that have added the oolong tea beverage that reclaims fragrant and all untapped relatively article 7 of enzyme processing make moderate progress, but natural sense, fresh feel are not almost played effect.

In addition; Added to use and reclaimed fragrant but carried out the natural sense, fresh feel of the oolong tea beverage of the relatively article 8 that protease and tannase handle and interpolation article not much at one; Though sweet taste, flavour, assorted flavor, happy sense more relatively article 7 make moderate progress, its evaluation is still poor.

In addition, particularly be aspect the flavor evaluation inequality of flavour, assorted flavor, happy sense etc. though added the oolong tea beverage that reclaims the fragrant relatively article 9 that do not use enzyme fully.

With respect to having added these relatively oolong tea beverages of article, the evaluation of having added the oolong tea beverage of the present invention's article is: natural sense, fresh feel, sweet taste, flavour, happy sense all increase, and assorted flavor reduces, excellent flavor.

The flavour of oolong tea beverage that has added the present invention's article 26 of handling through protease and tannase is strong especially, and also strong, the assorted flavor of sweet taste less in addition, happy sense is good.

In addition, added the oolong tea beverage of the present invention's article 27 of handling through glucoamylase and hemicellulase, all very strong, the assorted flavor of sweet taste, flavour that added the oolong tea beverage of the present invention's article 28 of handling through glucoamylase and pectase and added the oolong tea beverage of the present invention's article 29 of crossing through glucoamylase and cellulose treatment less, happy sense is good.

Claims (10)

1. the manufacturing approach of teas extract; It is characterized in that, (i) utilize steam distillation from the teas raw material, reclaim fragrance, (ii) to bottoms carry out that enzyme handle to obtain that enzyme is handled extract, the enzyme that (iii) operation obtained in (ii) handles that the recovery that obtains in extract and the operation (i) is fragrant to be mixed.

2. method according to claim 1 wherein, utilizes gas-liquid counter current contact extraction method to carry out steam distillation.

3. method according to claim 1 and 2, wherein, enzyme comprises protease and tannase.

4. method according to claim 1 and 2, wherein, enzyme comprises glucoamylase and hemicellulase.

5. method according to claim 1 and 2, wherein, enzyme comprises glucoamylase and pectase.

6. method according to claim 1 and 2, wherein, enzyme comprises glucoamylase and cellulase.

7. according to each described method of claim 4 ~ 6, wherein, enzyme does not have invertase activity in fact.

8. according to each described method of claim 1 ~ 7; Wherein, the enzyme that operation is obtained in (ii) handles the recovery perfume (or spice) that obtains in extract and the operation (i) and counts 1: 10 according to handle extract with enzyme with the mass ratio that reclaims perfume～and the interior ratio of scope was mixed in 10: 1.

9. according to each described method of claim 1 ~ 8, teas is at least a kind that is selected from azymic tea, semi-fermented tea and the fermented tea.

10. teas extract, it obtains through each described method of claim 1 ~ 9.