JP6990151B2 - Fragrance collection system, fragrance collection method, fragrance liquid, fragrance composition and compounded fragrance - Google Patents

Description

The present invention relates to an aroma collection system, an aroma collection method, an fragrance liquid, an aroma composition and a blended fragrance.

Conventionally, a steam distillation method, a solvent extraction method, or the like has been industrially used as a means for separating the aromatic component from animal and plant raw materials. In the field of aroma analysis research, headspace gas analysis is often used as a means of microanalysis, and after the aroma of fresh flowers is directly collected by an adsorbent, the aroma components are analyzed by gas chromatography or mass spectrometer. Component analysis is performed using the device. Such a fragrance analysis technique is introduced in, for example, "Use of gas chromatography and mass spectrometer in fragrance analysis" (Nishimura, Fragrance Journal, 1997-6, 12, 1997) and the like.

In the headspace gas analysis method, when the atmosphere of a fresh flower that emits only a weak fragrance is to be collected and analyzed by gas chromatography, air is sent to the fresh flower so as to be in continuous contact with the fragrance. A method of adsorbing and concentrating aroma components by contacting the air with an adsorbent such as silica gel or a porous polymer resin (dynamic headspace method; hereinafter referred to as DHS method) has been adopted.

On the other hand, the aqua space method (hereinafter referred to as the aqua space method (conventional method)) that more faithfully reproduces the aroma of fresh flowers and fruits than the DHS method is known (see Patent Document 1). Patent Document 1 describes an animal or plant raw material in which humidified air or an inert gas is brought into contact with the animal or plant raw material, the aroma of the animal or plant raw material is included in the humidified air or the inert gas, and then the aroma component is separated. The method of collecting the aroma of is described.

Japanese Unexamined Patent Publication No. 2000-5392

The method described in Patent Document 1 had a low aroma collection efficiency unless a cooling trap was used. Since it was necessary to use a cooling trap to improve the aroma collection efficiency, it was difficult to collect the aroma outdoors for a long time. Therefore, potted plants and cut flowers are placed in a device installed indoors to collect aroma. Depending on the type of plant, the aroma emitted by cutting flowers changes, and the aroma of the aromatic liquid (aroma concentrate) obtained by the aqua space method (conventional method) is the original aroma of the plant. It could be different.

An object to be solved by the present invention is to provide an aroma collection system and an aroma collection method capable of more efficiently collecting the original aromas of animals and plants and obtaining a natural fragrance liquid.
Further, the problem to be solved by the present invention is an fragrance liquid obtained by using these fragrance collection systems and fragrance collection methods, an fragrance composition obtained by analyzing the components of the fragrance liquid, and a fragrance composition thereof. It is to provide a blended fragrance which is a combination of an fragrance composition and another fragrance.

As a result of diligent research to solve the above problems, the present inventors have compared with the DHS method and the aqua space method (conventional method) by using a specific humidifying body and an adsorbent, and as a result, of animals and plants. We have found that the original fragrance can be collected more efficiently and a fragrance liquid with a natural feeling can be obtained, and the present invention has been completed.

The present invention, which is a specific means for solving the above problems, and a preferred embodiment thereof are as follows.
[1] It has a humidifying body generating part, an animal and plant raw material storage part, and an aroma component collecting part in this order.
The animal and plant raw material storage section communicates with the humidifying body generating section and the aroma component collecting section.
The humidifier generator generates a humidifier that is humidified air or an inert gas.
An aroma collection system in which the aroma component collection unit contains an adsorbent.
[2] The aroma collecting system according to [1], wherein the temperature of the aroma component collecting portion exceeds 0 ° C.
[3] The aroma collecting system according to [1] or [2], wherein the adsorbent is a hydrophobic adsorbent.
[4] The aroma collection system according to any one of [1] to [3], wherein the animal and plant raw material storage unit is a flexible bag.
[5] A gas inlet in which the flexible bag communicates with the humidifying body generator,
A bag mouth that can be fixed in contact with at least a part of animal and plant raw materials, and
The aroma collection system according to [4], which has a gas outlet that communicates with an aroma component collecting unit.
[6] The aroma collection system according to any one of [1] to [5], wherein the humidity of the humidifying body is higher than the humidity outside the system at the place where the aroma collection system is installed.
[7] The stage of introducing the humidified body generated in the humidified body generation part into the animal and plant raw material storage part and bringing it into contact with the animal and plant raw material.
Including the step of introducing a gas containing the aroma of animal and plant raw materials into the aroma component collecting section to collect the aroma components.
The humidifier is humidified air or an inert gas,
An aroma collection method in which the aroma component collecting unit contains an adsorbent.
[8] The step of preparing a humidifier by introducing air or an inert gas into the humidifier generator is included.
The aroma collecting method according to [7], wherein the step of preparing the humidifier is the step of introducing air or an inert gas into the water.
[9] The stage of preparing the humidifier is the stage of introducing the air outside the system into the humidifier generator, and
The aroma collecting method according to [8], wherein the humidity of the humidifying body is higher than the humidity outside the system.
[10] The method for collecting aroma according to any one of [7] to [9], which is at least a part of a plant in which animal and plant raw materials are growing.
[11] The animal and plant raw material storage section is a flexible bag.
The method for collecting aroma according to [10], which comprises a step of deforming a flexible bag to stimulate at least a part of a growing plant.
[12] Animal and plant raw materials are at least part of the plant,
At least some of the plants are in any one of [7]-[11], which is at least one of flowers, buds, vegetables, herbs, trees, bark, branches, leaves, buds, roots, and fruits. The described method for collecting aroma.
[13] The method for collecting aroma according to any one of [7] to [12], which comprises a step of passing an organic solvent through an adsorbent to obtain an aromatic liquid containing an aroma component.
[14] The fragrance liquid obtained by the aroma collection system according to any one of [1] to [6] or the aroma collection method according to any one of [7] to [13].
[15] An fragrance composition in which the composition of the fragrance liquid is reconstituted based on the component analysis results of the fragrance liquid according to [14].
[16] A blended fragrance that is a combination of the fragrance composition according to [15] and another fragrance.

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide an aroma collection system and an aroma collection method capable of more efficiently collecting the original aromas of animals and plants and obtaining a fragrance liquid having a natural feeling.
Further, according to the present invention, the fragrance liquid obtained by using these fragrance collection systems and fragrance collection methods, the fragrance composition obtained by analyzing the components of the fragrance liquid, and the fragrance composition thereof. It is possible to provide a blended fragrance in combination with other fragrances.

FIG. 1 is a schematic view of an example of the aroma collection system of the present invention. FIG. 2 is a gas chromatogram of a red hoppe aroma concentrate.

Hereinafter, the present invention will be described in detail. The description of the constituent elements described below may be based on typical embodiments and specific examples, but the present invention is not limited to such embodiments. In addition, the numerical range represented by using "-" in this specification means the range including the numerical values before and after "-" as the lower limit value and the upper limit value.

[Aroma collection system]
The aroma collecting system of the present invention has a humidifying body generating part, an animal and plant raw material storage part, and an aroma component collecting part in this order.
The animal and plant raw material storage section communicates with the humidifying body generating section and the aroma component collecting section.
The humidifier generator generates a humidifier that is humidified air or an inert gas.
The aroma component collecting part contains an adsorbent.
With this configuration, the original aroma of animals and plants can be collected more efficiently, and an aromatic liquid with a natural feeling can be obtained.
The novel aroma collecting method developed by the present inventors may be referred to as "an improved method of JP-A-2000-53992". The improved method of JP-A-2000-53992 differs from the aqua space method (conventional method) in that the aroma component is adsorbed on the adsorbent, and as a result, the aroma collection efficiency is improved without using a cooling trap. Be done.
When a specific humidified body such as humidified air is forcibly brought into contact with the animal or plant raw material, the humidified body well contains the original aroma of the animal or plant raw material. The reason is that the humidified body stimulates the animal and plant raw materials to make the animal and plant raw materials easy to emit fragrance, or the water vapor repeatedly adheres and volatilizes at the fragrance emitting site of the animal and plant raw materials, which results in room temperature. It is presumed that it acts like steam distillation below, but it is not clear.
Due to the above mechanism, in the aroma collecting system of the present invention, by using a specific humidifier and an adsorbent, the original aroma of animals and plants can be collected more efficiently, and an aromatic liquid having a natural feeling can be obtained. It is presumed that it will be obtained.
Hereinafter, preferred embodiments of the present invention will be described.

<Overall configuration of aroma collection system>
The overall configuration of the aroma collection system of the present invention will be described with reference to the drawings. FIG. 1 is a schematic view of an example of the aroma collection system of the present invention.
The aroma collecting system of the present invention has at least a humidified body generating unit 2, an animal and plant raw material storage unit 3, and an aroma component collecting unit 5 in this order, and the animal and plant raw material storing unit 3 has a humidified body generating unit 2 and an aroma component collecting unit. Communicate with the collection part 5.
With such an overall configuration aroma collection system, the humidified air or inert gas is forcibly brought into contact with the animal and plant raw materials to include the aroma component in the humidified body, and the humidified body is removed from the animal and plant raw material storage. The aroma can be collected by taking it out and adsorbing the aroma component on the adsorbent. An aromatic liquid (aroma concentrate) can be obtained by desorbing the aroma component from the adsorbent on which the aroma component is adsorbed using an organic solvent (diethyl ether, etc.) and concentrating it if necessary. Desorption and enrichment are not shown in the drawings.

An example of the aroma collection system shown in FIG. 1 is a preferred embodiment of the aroma collection system of the present invention. Hereinafter, preferred embodiments of the aroma collection system of the present invention will be described with reference to FIG. 1, but the description of FIG. 1 is not limited to the present invention.
In FIG. 1, the blower 1 and the blower 8 are fixed to the base 10, respectively.
In FIG. 1, the gas 11 outside the system of the aroma collection system is introduced into the humidifying body generation unit 2 by the blower 1, and the humidifying body 12 is generated by the humidifying body generating unit 2. It is preferable to provide a cleaning unit 9 between the blower 1 and the humidifying body generating unit 2. The humidifying body generating unit 2 is provided with clean water 7 such as pure water, and the humidifying body 12 can be generated by passing the gas 11 through the water 7. In FIG. 1, the humidifying body generating portion 2 is fixed to a part of the animal and plant raw materials 4 via a fixing member 21.
The humidifying body 12 generated in the humidifying body generating unit 2 is introduced into the animal and plant raw material storage unit 3 and is forcibly brought into contact with the animal and plant raw material 4 (in FIG. 1, the branch in which Oshima cherry blossoms are in bloom). The animal and plant raw material storage unit 3 has a gas inlet 31 that communicates with the humidified body generation unit 2, a bag mouth portion 32 that can be fixed in contact with a part of the animal and plant raw material 4 (branches of Oshima cherry tree), and an aroma component collection. It has a gas discharge port 33 communicating with the part 5.
The humidifying body 12 that has come into contact with the animal and plant raw material 4 inside the animal and plant raw material storage unit 3 is sucked by the blower 8 and taken out from the animal and plant raw material storage unit 3 and introduced into the aroma component collecting unit 5. The extracted humidified body 12 passes through the adsorbent 6 contained in the aroma component collecting unit 5, and at that time, the aroma component contained in the humidified body 12 including the aroma (aroma component) of the animal and plant raw materials is the adsorbent 6. Is adsorbed on. The humidifying body 12 that has passed through the adsorbent 6 is sucked by the blower 8, taken out from the aroma component collecting unit 5, and discharged to the outside of the system of the aroma collecting system via the blower 8.

Further, industrially, the aroma collection system as shown in FIG. 1 can be scaled up and used. For example, the animal and plant raw material storage portion may be a closed type, and the inside may be multi-staged, and the animal and plant raw material may be placed on each stage so that a larger amount of aromatic liquid can be obtained.

The material of each part of the aroma collection system is not particularly limited, but it is preferably one that does not have an offensive odor or one that does not generate an offensive odor due to a humidifier. For example, a resin such as PEP and acrylic resin, a metal such as stainless steel, and glass can be exemplified.
Hereinafter, preferred embodiments of each part of the aroma collection system will be described.

<Blower and exhaust fan>
There are no particular restrictions on the blower and exhaust fan. A normal pump can be used. The flow rate (air volume) of the gas blown by the blower can be changed according to the purpose of aroma collection and the size of the aroma collection system. For example, for the purpose of collecting aromas of flowers, fruits and herbs, the air volume is preferably 0.1 to 10.5 L / min, more preferably 0.5 to 5.5 L / min. It is particularly preferably 1.0 to 3.5 L / min.
On the other hand, the flow rate (exhaust amount) of the gas exhausted by the blower can be set according to the amount of blown air. For example, the exhaust air volume is preferably 0.1 to 10 L / min, more preferably 0.5 to 5 L / min, and particularly preferably 1.0 to 3.0 L / min.
It is preferable that the amount of air blown and the amount of exhausted air are at least these lower limit values from the viewpoint of sufficiently adsorbing the aroma component to the adsorbent. The viewpoint that the amount of air blown and the amount of exhaust air are not more than these upper limit values suppresses the humidifier from passing through the adsorbent before the aroma component is adsorbed on the adsorbent, and makes it easier for the adsorbent to adsorb the aroma component. , And, it is preferable from the viewpoint of suppressing the load applied to the flow path from the blower to the exhaust fan and making it difficult for the flow path to come off.
The amount of air blown is preferably the same as the amount of exhaust air when the bag mouth of the animal and plant raw material storage portion and the animal and plant raw material are sealed.
On the other hand, when the bag mouth of the animal and plant raw material storage portion and the animal and plant raw material are not sealed, it is preferable that the amount of air blown is larger than the amount of exhaust air. A bag mouth is provided at the part outside the flow path (end, etc.) from the gas inlet to the gas outlet of the animal and plant raw material storage part, and the amount of air blown is larger than the amount of exhaust air, so that the humidified body is mainly a flexible bag. It is passed from the gas inlet to the gas outlet. In addition, it is possible to prevent the gas outside the system from flowing into the animal and plant raw material storage portion from the bag mouth portion and collecting the odor derived from the gas outside the system.
The blast or exhaust may be continuous or interrupted in the middle. It is industrialized to continuously collect the aroma of animal and plant raw materials for many days by interrupting the ventilation or exhaust air, replacing the animal and plant raw materials (such as cut flowers) with fresh ones, and then restarting the collection. It is preferable from the viewpoint of.
The blower and the blower may be fixed at a height of 1 m or more from the ground (preferably in the range of 1 to 3 m from the ground) by being installed on a table or the like. It can be preferably applied when the animal and plant raw materials are plants having a height of 1 m or more from the ground.

<Humidifier generator>
In the present invention, the humidifying body generator generates a humidified body which is humidified air or an inert gas. The humidified body generated in the humidified body generating part is introduced into the animal and plant raw material storage part and brought into contact with the animal and plant raw materials. By using a humidifier, it becomes easier to collect the original aroma of animal and plant raw materials.

(Humidifier)
The humidifier is humidified air or an inert gas.
As the air, air outside the system of the aroma collection system can be used.
As the inert gas, helium, nitrogen, argon or the like can be used, but nitrogen is the most economical and useful.
Whether to use air or an inert gas needs to be appropriately selected depending on the animal and plant raw materials for which the aroma is to be collected. For example, when the animal and plant raw materials are fresh flowers and fruits (flowers and fruits of growing plants that suck up nutrients from the roots) and cut flowers, it is preferable to use air so that the animal and plant raw materials do not suffocate and die. ..

It is preferable that the humidity of the humidifier is higher than the humidity outside the system at the place where the aroma collection system is installed. When a humidified body is introduced into the air outside the system, the temperature of the humidified body is preferably the same as that of the air outside the system, and the humidity of the humidified body is preferably higher than that of the air outside the system. On the other hand, when an inert gas is introduced as the humidifying body, it is preferable to adjust the temperature of the humidifying body to be the same as that of the air outside the system, and it is preferable that the humidity of the humidifying body is higher than that of the air outside the system. ..
The humidity of the humidifier is preferably 40 to 100%, more preferably 60 to 100%, and particularly preferably 80 to 100%.
The temperature of the humidifying body is preferably more than 0 ° C. and 50 ° C. or lower, more preferably 15 to 45 ° C., and particularly preferably 20 to 40 ° C. The temperature of 50 ° C or lower is preferable from the viewpoint of making it difficult for the aroma of the animal and plant raw materials to be changed by heating, and the temperature of 40 ° C or lower is preferable when the animal and plant raw materials such as fresh flowers, fresh fruits and cut flowers are used. It is preferable from the viewpoint of making it difficult. The temperature exceeding 0 ° C. is preferable from the viewpoint of increasing the humidity, and the temperature of 15 ° C. or higher is preferable from the viewpoint of facilitating the preparation of a humidified body having a humidity of 80% or more.
In particular, using air with a temperature of 15 to 40 ° C and a humidity of 80% or more is a suitable condition for maintaining the freshness of cut flowers, so that the cut flowers do not die and can withstand long-term aroma collection. be able to.

<Cleaning section>
It is preferable to provide a cleaning unit on the upstream side of the animal and plant raw material storage unit to purify the gas outside the system. By providing the purification unit, it is possible to collect the faint aroma that contributes to the original aroma of animals and plants, while preventing the collection of various other volatile components existing in the atmosphere outside the system.
The gas purifying section outside the system can be provided between the outside of the system and the blower, between the blower and the humidifying body generating section, and the like. However, the humidifying body cleaning section may be provided between the humidifying body generating section and the animal and plant raw material storage section. It is preferable to provide a gas purifying section outside the system between the blower and the humidifying body generating section. If necessary, a cleaning section may be provided between the outside of the system and the blower, but an outside gas (air) introduced into the humidifying body generating section by providing a cleaning section immediately before the humidifying body generating section. Alternatively, it is preferable to purify the inert gas).
The cleaning part is preferably a filling part of a material that contributes to deodorization and the like. The material that contributes to deodorization is not particularly limited, and examples thereof include materials used for the adsorbent described later. Activated carbon is more preferable from the viewpoint of collection efficiency of volatile components.

<Animal and plant raw material storage section>
The animal and plant raw material storage unit is not particularly limited as long as it can store the animal and plant raw materials. It is preferable that animal and plant raw materials can be freely stored and taken out.

(Animal and plant raw materials)
The raw material for animals and plants is not particularly limited, and may be an animal or a plant. In the present invention, it is preferable that the animal and plant raw materials are at least a part of the plant. However, the animal and plant raw materials may be materials derived from non-growing plants or animals. Examples of non-growing plants include cut flowers, stripped fruits, processed plants (for example, roasted coffee beans, teas such as black tea, spices, plants used in Chinese herbs) and the like. Examples of animal-derived materials include fish and shellfish, livestock meat, and processed foods thereof.

At least some of the plants used as animal and plant raw materials are at least among flowers, buds, vegetables, herbs, trees, bark, branches, leaves, buds, roots and fruits (including false fruits such as strawberries in the present specification). It is preferably one type. Two or more of these may be used, and examples thereof include branches with flowers. Among these, flowers, buds, herbs, and fruits are more preferable from the viewpoint of analyzing the aromatic liquid of the present invention and using the compounded fragrance of the present invention described later for fragrance and flavor applications.
Examples of flowers and buds include potted flowers such as roses, tin orchids, jasmine, lilies, gardenia, orchids, and sweet osmanthus; and flowers of trees such as cherry blossoms, sea urchins, wisteria, magnolias, magnolia magnolias, and sweet osmanthus.
Examples of vegetables include vegetables used in Chinese herbs such as lily root, licorice, turmeric, Japanese pepper, and ginseng. Vegetables are a general term for herbs used as side dishes, and root vegetables such as carrots are also included in vegetables.
Examples of the tree and bark include those that generate fragrance such as cinnamon, cedarwood, agarwood, and cara.
The branches, leaves, and buds are not particularly limited, and even if they generate fragrance by themselves, they do not generate fragrance by themselves (mainly non-fragrant parts such as branches with flowers). May be good.
Examples of the root include iris root.
Examples of herbs include plants used for spices such as mint, lemongrass, geranium, thyme, lavender, and basil.
Examples of fruits include strawberries, apples, peaches, mandarins, and pears.

Since the present invention can efficiently collect the original aroma of animals and plants without using a cooling trap, it can be applied particularly to tree flowers and large flowers among potted flowers, and specifically, animal and plant raw materials. It can also be applied when at least a part of the plant used as is present at 1 m or more (for example, about 1 to 3 m) from the ground. Further, the present invention can be applied to plants growing outdoors because the original aromas of animals and plants can be efficiently collected without using a cooling trap. In this case, the animal and plant raw material storage portion may be fixed at a height of 1 m or more from the ground.
The present invention can also be applied to the case where the animal and plant raw materials are plants lower than 1 m, and in that case, the position of the aroma component collecting portion is not limited. Examples of plants whose raw materials for animals and plants are less than 1 m include strawberries and basil.

(Characteristics of animal and plant raw material storage)
The animal and plant raw material storage portion is preferably flexible. Since the animal and plant raw material storage portion has flexibility, the target aroma of the animal and plant raw material can be collected more efficiently in a natural environment. In particular, when the target aroma of animal and plant raw materials is in a specific situation (when the aroma is strongly generated when growing) and at a specific timing (when the aroma is generated at a certain time during the day), the moon is under the moon. When it is artificially difficult to collect flowers that bloom in the middle of the night, such as beautiful women), or when it can only be obtained under specific conditions (such as herbs that stimulate and generate aroma). , It becomes easier to obtain the desired aroma.
Specifically, the flexible animal and plant raw material storage portion is preferably a flexible bag.
There are no particular restrictions on the material of the animal and plant raw material storage section. For example, resin, metal, glass can be mentioned. Among these, a flexible resin or metal is preferable. Examples of the resin include vinyl alcohol-based polymers, polyvinyl fluoride, polyvinylidene fluoride, and polyethylene. Examples of the metal include aluminum and stainless steel. Among these, the material of the animal and plant raw material storage part is preferably a vinyl alcohol-based polymer. For example, as an example of a flexible bag which is a vinyl alcohol polymer, "Smart Bag PA" manufactured by GL Sciences Co., Ltd. can be mentioned.

(Structure of animal and plant raw material storage)
The animal and plant raw material storage unit may be a partially open container or a closed container as shown in FIG. 1, and is used according to the purpose. For example, in the case of growing animal and plant raw materials, it is preferable to use a partially open container, and in the case of non-growing animal and plant raw materials, a closed container is preferable.
Hereinafter, a preferred embodiment of the structure of the animal and plant raw material storage unit will be described by taking the case where the animal and plant raw material storage unit is a flexible bag as a typical example. The flexible bag is preferably a partially open container. Specifically, the flexible bag communicates with the gas inlet communicating with the humidified body generating portion, the bag mouth portion that can be fixed in contact with at least a part of the animal and plant raw materials, and the aroma component collecting portion. It is preferable to have a gas outlet.
The bag mouth portion and the animal and plant raw materials may be fixed in an open state with a part of the bag mouth portion open, or may be sealed (sealed) in order to improve the collection efficiency of the aroma component.

<Aroma component collection section>
The aroma component collecting portion is not particularly limited as long as it contains an adsorbent. Examples thereof include a general column that can be filled with an adsorbent.
In the present invention, it is preferable that the temperature of the aroma component collecting portion exceeds 0 ° C. Further, it is more preferable that the minimum temperature in the flow path from the animal and plant raw material storage section to the aroma component collecting section exceeds 0 ° C. By substantially not having a cooling trap in the flow path from the animal and plant raw material storage section to the aroma component collecting section, the aroma yield of the adsorbent can be increased. Further, when the minimum temperature in the flow path from the animal and plant raw material storage section to the aroma component collecting section exceeds 0 ° C., freezing of water in the humidifier can be suppressed and blockage of the channel can be suppressed.
The aroma component collecting portion may be fixed at a height of 1 m or more from the ground. It can be preferably applied when the animal and plant raw materials are plants having a height of 1 m or more from the ground.

(Adsorbent)
There are no particular restrictions on the adsorbent. For example, silica gel, porous resin, activated carbon and the like can be mentioned. Examples of the porous resin include 2,6-dimethyl-paraphenylene oxide polymer, 2,6-diphenylene-paraphenylene oxide polymer, acrylic ester polymer, and styrenedivinylbenzene polymer.
The adsorbent may be a hydrophobic adsorbent or a hydrophilic adsorbent, but a hydrophobic adsorbent is preferable.
It is more preferable that the adsorbent contains a 2,6-diphenylene-paraphenylene oxide polymer.
The number of meshes of the porous adsorbent is not particularly limited.
The amount of the adsorbent filled in the aroma component collecting portion is not particularly limited.

<How to use the aroma collection system>
In one of the preferred embodiments of the aroma collection system, the entire aroma collection system may be installed outdoors. This method of use is particularly preferred when the animal and plant raw material is a tree. In order to improve the aroma collection efficiency by the method described in JP-A-2000-53992, a cooling trap (ice cooling trap, trap cooled with dry ice in ethanol, liquid nitrogen trap) is used. Since it is necessary to collect aroma for a long time, it is difficult to collect aroma outdoors for a long time. Therefore, in the method described in JP-A-2000-53992, a plant in the state of a potted plant or a cut flower is placed in a device installed indoors to collect aroma. Depending on the type of plant (flower), the aroma emitted by cutting flowers may change, and the aroma concentrate obtained by the conventional method may differ from the original aroma of the plant. On the other hand, according to one of the preferred embodiments of the present invention, the headspace gas scent is collected for a long time using a humidifying body even for a plant growing outdoors without damaging it. It is possible to obtain an aroma concentrate having the original scent of a plant (flower).
However, a part or the whole of the aroma collection system may be installed indoors. This method of use is particularly preferable when the raw materials for animals and plants are potted plants, plants whose fragrance does not change even when cut flowers are used, and fruits that have been peeled off.

[Aroma collection method]
The aroma collecting method of the present invention includes a stage in which the humidified body generated in the humidified body generating part is introduced into the animal and plant raw material storage part and brought into contact with the animal and plant raw materials.
Including the step of introducing a gas containing the aroma of animal and plant raw materials into the aroma component collecting section to collect the aroma components.
The humidifier is humidified air or an inert gas,
The aroma component collecting part contains an adsorbent.
Hereinafter, preferred embodiments of the aroma collecting method of the present invention will be described.

<Stage of preparing a humidifier>
The aroma collecting method preferably includes a step of introducing air or an inert gas into the humidifying body generating portion to prepare the humidifying body.
The method of generating the humidified body is not particularly limited. For example, it can be generated by blowing air or an inert gas into a solvent containing water. The solvent containing water is preferably water having a small amount of volatile components other than water, more preferably odorless and clean water, and particularly preferably ultrapure water (so-called Milli-Q water). The step of preparing the humidifier is preferably the step of introducing air or an inert gas into the water.
When a humidifying body cleaning part (for example, an activated carbon filling part) is provided between the humidifying body generating part and the animal and plant raw material storage part, the solvent containing water contains any volatile component or non-volatile component. You may be.
The temperature of the water used is not particularly limited and may be, for example, 0 to 50 ° C, preferably 15 to 45 ° C, and more preferably 20 to 40 ° C.
In the present invention, it is preferable that the stage of preparing the humidifying body is the stage of introducing the air outside the system into the humidifying body generating portion, and the humidity of the humidifying body is higher than the humidity of the outside system.

<Stage of storing animal and plant raw materials>
The aroma collection method preferably includes a step of storing animal and plant raw materials.
There are no particular restrictions on the method of storing animal and plant raw materials. When using a partially open container such as a flexible bag with a bag mouth, part or all of the animal and plant raw materials are stored inside the flexible bag, and then a part of the bag mouth is fixed in an open state. It can be opened to the outside of the system, but the bag mouth may be sealed (sealed) if necessary.
When a closed container such as a glass container provided with a lid and a main body is used, the animal and plant raw materials can be stored in the main body of the container and then the lid can be closed and sealed.

<Stimulating stage>
In one preferred embodiment of the aroma collection method, the animal and plant raw material storage is a flexible bag, which comprises a step of deforming the flexible bag to stimulate at least a part of a growing plant. Is preferable. By deforming the flexible bag and stimulating the growing plant, it is possible to carry out a method of positively generating aroma in the animal and plant raw materials. This method could not be easily realized in the case of the method described in JP-A-2000-53992 (when the animal and plant raw material storage portion does not have flexibility). In particular, when the animal and plant raw materials are stimulated multiple times and / or for 2 seconds or longer, the flexible bag can be used to easily stimulate the animals and plants.

<Stage of collecting aroma components>
The aroma collecting method preferably includes a step of collecting aroma components.
There is no particular limitation on the method of collecting aroma components. The aroma component can be collected by introducing the humidifier taken out from the animal and plant raw material storage section into the aroma component collecting section containing the adsorbent. In the present invention, it is preferable to suck the humidified body with a blower and forcibly take it out from the animal and plant raw material storage section.
The collection time is not particularly limited. In the present invention, since the aroma collection efficiency can be improved without using a cooling trap, the aroma component can be collected for a long time easily and at low cost. In particular, when collecting aroma components outdoors, the aroma components can be collected for a long time at low cost. Therefore, the collection time can be, for example, 3 hours or more, and if necessary, 5 hours or more.

<Stage of obtaining fragrance liquid>
The aroma collecting method preferably includes a step of passing an organic solvent through an adsorbent to obtain an aroma liquid containing an aroma component. Passing an organic solvent through the adsorbent is also called desorption.
As the organic solvent, hydrocarbons such as pentane, ethers and the like can be used, and if necessary, one or two or more organic solvents can be used.
At the stage of obtaining the fragrance liquid, it is preferable to further purify, wash and concentrate the desorbed liquid obtained by passing an organic solvent through the adsorbent.

[Aroma liquid]
The fragrance liquid of the present invention is obtained by the aroma collection system of the present invention or the aroma collection method of the present invention. The scent of the fragrance liquid of the present invention may be a scent that reproduces the literal "freshness" like the scent of a flower floating in the morning mist.
The fragrance liquid of the present invention is useful for aroma component analysis research, and can be used for analysis (abbreviated as GC-MS) by combining gas chromatography (GC) and mass spectrometry (MS), or for sensory evaluation. , You can adjust the scent by referring to the scent. The gas chromatograph used for GC is preferably equipped with a FID (Flame Ionization Detector) detector, and it is preferable to combine this with a mass spectrometer for GC-MS / FID analysis.
Further, the aromatic liquid of the present invention can be used in the aromatic composition of the present invention described later. On the other hand, the fragrance liquid of the present invention is industrially useful for applications such as cosmetics and flavors.

[Aroma composition]
The fragrance composition of the present invention is obtained by reconstructing the composition of the fragrance liquid of the present invention based on the component analysis result of the fragrance liquid of the present invention.
Based on the aroma analysis result of the aromatic liquid of the present invention, an aromatic composition that reproduces the composition can be obtained.

[Mixed fragrance]
The blended fragrance of the present invention is a combination of the fragrance composition of the present invention and other fragrances. The blended fragrance of the present invention can be used for fragrance applications and flavor applications.

Hereinafter, the present invention will be described in more detail with reference to Examples and Comparative Examples. The materials, amounts used, ratios, treatment contents, treatment procedures, etc. shown in the following examples can be appropriately changed as long as they do not deviate from the gist of the present invention. Therefore, the scope of the present invention should not be construed as limiting by the specific examples shown below.

[Example 1]
<Improvement method of Oshima cherry tree, outdoor, Japanese Patent Application Laid-Open No. 2000-53992 (method for collecting aroma of the present invention)>
Using the aroma collection system of the present invention exemplified in FIG. 1, the aroma collection method of the present invention was carried out.
The aroma collection system used has a configuration in which a blower, a purifier, a humidifier generator, an animal and plant raw material storage unit, an aroma component collection unit, and a blower are communicated in this order. Each part was placed and fixed outdoors and at a height of 1 to 3 m from the ground. The blower and the blower were fixed to the table, the humidifying body generating part was fixed to a part of the animal and plant raw materials via a fixing member, and the animal and plant raw material storage part was fixed to the animal and plant raw materials by the method described later.
Pumps were used as blowers and exhausters.
As the humidifier generator, the humidifier generator described in [0018] of JP-A-2000-53992 was used. Odorless clean water was placed in the humidifier generator so that the humidifier could be supplied by introducing gas into the water and bubbling. In addition, activated carbon as a purifying part for gas outside the system was placed at the entrance of the humidifying body generating part.
A flexible bag (“Smart Bag PA” manufactured by GL Sciences Co., Ltd.), which is a vinyl alcohol polymer, was used as a storage unit for animal and plant raw materials. The flexible bag is provided with a gas inlet that communicates with the humidified body generating portion, a bag mouth portion, and a gas outlet that communicates with the aroma component collecting portion.
Oshima cherry blossoms growing outdoors in Kanagawa Prefecture were used as raw materials for animals and plants. Oshima cherry is a variety that gives off a faint fragrance from the petals.
A column filled with an adsorbent was used as an aroma component collecting part.
As an adsorbent, Tenax TA (2,6-diphenylene-paraphenylene oxide polymer), 35/60 mesh, 2.0 g was used.

A part of the branch with about 20 Oshima cherry blossoms was wrapped in a flexible bag and stored, and the bag mouth was lightly contacted with the branch and fixed without sealing. The air outside the system was supplied to the humidifying body generating part by a blower to obtain humidified air. The obtained humidified air is introduced into the gas inlet of the flexible bag (located on the vertically upper surface of the flexible bag and on the portion vertically below the bag mouth), and the head of the flexible bag is introduced. The space gas was sucked out from the gas discharge port (located on the bottom surface of the flexible bag opposite to the bag mouth) through the aroma component collecting part by a blower. By this step, humidified air passes mainly from the gas inlet of the flexible bag toward the gas outlet, and the aroma component is collected by the adsorbent. No cooling trap was used. Other conditions are as follows.
-Flow rate: Blower (humidifier) 3.0 L / min, exhaust (suction) 2.5 L / min.
・ Collection time: 3 hours during the day in April.
-Temperature (outside the system): 19-21 ° C.
-Temperature (inside the system): Same as outside the system. The temperature of the humidifying body generating part and the aroma component collecting part is also not controlled.
Next, 20 mL of diethyl ether was passed through the adsorbent to desorb the aroma component. The desorbed liquid was dehydrated with Glauber's salt (Na ₂ SO _{4.10H 2} O), filtered through a filter paper, and concentrated at 43 ° C. under normal pressure to obtain the aromatic liquid (fragrance concentrate) of Example 1.
The fragrance solution of Example 1 was subjected to GC-MS / FID analysis.

[Comparative Example 1]
<Oshima cherry tree / outdoor / DHS method>
Example 1 except that the blower and the humidifier generator are not used, the humidifier is not supplied, and the gas inlet is opened to the air outside the system without using activated carbon as a gas purifier outside the system. In the same manner, the aroma collecting method of Comparative Example 1 was performed. The obtained fragrance liquid was subjected to GC-MS / FID analysis in the same manner as in Example 1.
The pump exhausted only the air (suction), and the flow rate of the exhaust air was set to 2.5 L / min.
・ Collection time: 3 hours during the day in April.

[Comparative Example 2]
<Oshima Sakura / Indoor / Aqua Space Method (conventional method)>
A flower of Oshima cherry tree (the same tree as in Example 1) cut out together with a branch and inserted into ultrapure water (Milli-Q water) (cut branch) was used as an animal and plant raw material, and FIG. 1 of JP-A-2000-53992 The aroma was collected by the aqua space method (conventional method) using the device described in 1. A glass container was used as an animal and plant sample storage unit. The glass container has a gas inlet (located on the side surface of the vertically upper part of the glass container) that communicates with the humidified body generating part via the cleaning part, and a gas outlet (located on the side surface of the vertical upper part of the glass container) that communicates with the aroma component collecting part. (Placed on the side of the vertical lower part) was provided so that the other parts could be sealed. Using two pumps, headspace gas is sucked while introducing humidified air under the following conditions, and aroma components are collected and condensed by a cooling trap (a trap cooled with dry ice in ethanol). A liquid (5.1 g) was obtained.
-Flow rate: Blower (humidifier) 2.5 L / min, exhaust (suction) 2.5 L / min.
・ Collection date and time: 3 hours during the day in April.
After recovering the condensed liquid (5.1 g), all the walls of the cooling trap were washed with 20 mL of diethyl ether, and the entire amount of the obtained liquid (the liquid obtained by washing) was mixed with the condensed liquid. After adding 0.8 g of sodium chloride to the obtained mixed solution and dissolving it, the solution was separated and the aqueous layer was extracted twice with 5 mL of ether. After the ether layers were combined and dehydrated with Glauber's salt, the mixture was filtered through filter paper and concentrated at 43 ° C. under normal pressure to obtain the aromatic liquid (fragrance concentrate) of Comparative Example 2. The obtained fragrance liquid was subjected to GC-MS / FID analysis in the same manner as in Example 1.

[Comparative Example 3]
<Oshima Sakura / Indoor / DHS method>
The flowers of Oshima cherry tree (the same tree as in Example 1) were cut out together with the branches and inserted into ultrapure water (Milli-Q water) (cut branches) as animal and plant raw materials, and the aroma of Comparative Example 3 was collected by the DHS method. Made the way. A glass container was used as an animal and plant sample storage unit. The glass container has a gas inlet (located on the side surface of the vertically upper part of the glass container) that communicates with the air outside the system through the cleaning section, and a gas outlet (located on the side surface of the vertical upper part of the glass container) that communicates with the aroma component collecting section. (Placed on the side of the vertical lower part) was provided so that the other parts could be sealed. Headspace gas was sucked while introducing air under the following conditions using one pump, and aroma was collected to obtain the fragrance liquid (aroma concentrate) of Comparative Example 3. The obtained fragrance liquid was subjected to GC-MS / FID analysis in the same manner as in Example 1.
The pump exhausted only the air (suction), and the flow rate of the exhaust air was set to 2.5 L / min.
・ Collection date and time: 3 hours during the day in April.

[evaluation]
<Sensory evaluation>
The fragrance liquids (fragrance concentrates) of Examples 1 and Comparative Examples 1 to 3 were subjected to sensory evaluation by 10 well-trained professional panelists. The results are shown below.
Evaluation criteria: 10 points for the most "favorable scent" or "similar to the scent of Oshima cherry blossoms", 0 points for the most "unfavorable scent" or "not similar to the scent of Oshima cherry blossoms", ordinary The 11-grade evaluation with 5 points was scored in 1-point increments, and the sensual characteristics were described.
Table 1 below shows the average score and the average sensory evaluation of the evaluation points of 10 professional panelists.

＊１：パウダリー感について：広山均著、「フレグランス 香りのデザイン」、平成１２年１月２０日（２０００）第１版、フレグランスジャーナル社発行、ｐ１６９によれば、パウダリーノート（Ｐｏｗｄｅｒｙ Ｎｏｔｅ）とは、白粉のように、甘く粉っぽいにおいである（例、クマリン、バニリン、ヘリオトロビンなど）。

* 1: About powdery feeling: According to Hitoshi Hiroyama, "Fragrance Fragrance Design", January 20, 2000 (2000) 1st Edition, published by Fragrance Journal, p169, what is Powdery Note? It has a sweet, powdery odor, like white powder (eg, coumarin, vanillin, heliotrobin, etc.).

From Table 1 above, the aromatic liquid obtained by performing the aroma collection method on the Oshima cherry blossoms (cut branches) of Comparative Examples 2 and 3 has a perilla-like metallic feeling and a negative aroma such as a stuffy odor. I felt it. No negative scent was felt in the fragrance liquid obtained by performing the scent collection method outdoors on the Oshima cherry blossoms growing outdoors in Example 1 and Comparative Example 1, but from Comparative Example 1. In Example 1, the scent of cherry blossoms had a more natural feeling, and the average score was also high.

<Aroma component>
Table 2 shows the abundance ratio (GC-Area%) of the main aroma components for the GC-MS / FID analysis results of the aromatic liquids (aroma concentrates) of Examples 1 and Comparative Examples 1 to 3.

From Table 2 above, in Example 1, fresh floral linalool and rose-like 2-phenylethanol (2-phenylethanol) were detected at a higher rate than in Comparative Examples 1 to 3, and further, anise-like anisaldehyde was detected. (Anisaldehyde) and methyl p-anisate were detected in higher proportions than in Comparative Examples 2 and 3.

<Summary of evaluation results of Oshima cherry blossoms>
Regarding the method of collecting the scent of Oshima cherry blossoms, when the scent of cut flowers is collected using the aqua space method (conventional method) of Comparative Example 2, an aromatic liquid (fragrance concentrate) containing a stuffy odor (fatty odor) is obtained. was gotten.
On the other hand, it was found that by using the aroma collection system and the aroma collection method of the present invention, a fragrance liquid (aroma concentrate) having a natural feeling closer to the aroma of a real flower can be obtained.
Based on the above evaluation results, regarding the method for collecting the aroma of Oshima cherry blossoms, the method for collecting the aroma of the present invention performed outdoors in Example 1, that is, the method for collecting the aroma by the improved method of JP-A-2000-53992. Is considered to be effective.

[Example 101]
<Improved method of Beni Hoppe / Indoor / Japanese Patent Application Laid-Open No. 2000-53992 (method for collecting aroma of the present invention)>
The aroma collection system used in Example 1 was modified as follows to be the aroma collection system of Example 101, and the aroma collection method of the present invention was carried out using the aroma collection system of Example 101. ..
A glass container was used as a storage unit for animal and plant raw materials. The glass container has a gas inlet (located on the side surface of the vertically lower part of the glass container) that communicates with the humidified body generator and a gas outlet (located on the lid of the vertically upper part of the glass container) that communicates with the aroma component collecting part. ) Was provided so that the other parts could be sealed. The entire aroma collection system was placed and fixed indoors and at a height of 1 to 2 m from the ground. The blower, the blower, and the humidifying body generating part were fixed to the table, and the animal and plant raw material storage part was fixed to the table with the animal and plant raw materials covered.
As a raw material for animals and plants, strawberry (which has a red hoppe, a false fruit part and a calyx part), which is a peeled fruit, was used.
As an adsorbent, Tenax TA (2,6-diphenylene-paraphenylene oxide polymer), 60/80 mesh, 2.0 g was used.

Eight red cheeks were placed inside a glass container, and all but the gas inlet and gas outlet were sealed. The air outside the system was supplied to the humidifying body generating part by a blower to obtain humidified air. The obtained humidified air is introduced into the gas inlet of the glass container (located on the lower side surface of the glass container), and the headspace gas of the glass container is introduced from the gas outlet (located on the lid at the vertically upper part of the glass container). It was taken out by sucking it with a blower through the collection part. By this step, the aroma component is collected in the adsorbent. No cooling trap was used.
-Flow rate: Blower (humidifier) 1.5 L / min, exhaust (suction) 1.5 L / min.
・ Collection time: 4 hours ・ Room temperature (outside the system): 21 ° C
-Temperature (inside the system): Same as outside the system. The temperature of the humidifying body generating part and the aroma component collecting part is also not controlled.
・ Humidity: (outside the system) 42%
Next, 20 mL of diethyl ether was passed through the adsorbent to desorb the aroma component. After dehydrating the desorbed solution with Glauber's salt, the solution was filtered through a filter paper and concentrated at 43 ° C. under normal pressure to obtain 42.7 mg (including solvent) of the fragrance solution (fragrance concentrate) of Example 101.
The fragrance solution of Example 101 was subjected to GC-MS / FID analysis.

[Reference Example 101]
<Checking the temperature and humidity of the humidifier>
As a result of conducting the same experiment as in Example 101 on another day, it was confirmed that the air outside the system and the air (humidifier) introduced into the system had the following temperatures and humidity, respectively. That is, it was found that the temperature of the humidifier was the same as the temperature outside the system at the place where the humidifier was installed, and the humidity of the humidifier was higher than the humidity outside the system at the place where the aroma collection system was installed.
<At the start>
Exoplanet: 17.3 ° C, humidity 47%
In the system: 17.3 ° C, humidity 47%
<60 minutes later>
Exoplanet: 17.9 ° C, humidity 51%
In the system: 17.9 ° C, humidity 85%

[Comparative Example 101]
<Red Hoppe / Indoor / Aqua Space Method (conventional method)>
Eight red hoppe were used as raw materials for animals and plants, and the same apparatus shown in FIG. 1 of JP-A-2000-53992 as in Comparative Example 2 was used indoors to collect aroma by the aqua space method (conventional method). A glass container was used as an animal and plant sample storage unit. Using two pumps, headspace gas is sucked while introducing humidified air under the following conditions, and aroma components are collected and condensed by a cooling trap (a trap cooled with dry ice in ethanol). A liquid (4.7 g) was obtained.
-Flow rate: Blower (humidifier) 1.5 L / min, exhaust (suction) 1.5 L / min.
・ Collection time: 4 hours ・ Room temperature (outside the system): 21 ° C
-Temperature (inside the system): Same as outside the system. The temperature of the humidifying body generating part and the aroma component collecting part is also not controlled.
-After recovering the condensed liquid (4.7 g), all the walls of the cooling trap were washed with 20 mL of diethyl ether, and the entire amount of the obtained liquid was mixed with the condensed liquid. After 0.7 g of sodium chloride was added to the obtained mixed solution to dissolve it, the solution was separated, and the aqueous layer was extracted twice with 5 mL of ether. After the ether layers were combined and dehydrated with Glauber's salt, the mixture was filtered through filter paper and concentrated at 43 ° C. under normal pressure to obtain 42.9 mg (including solvent) of the aromatic liquid (fragrance concentrate) of Comparative Example 101. The obtained fragrance liquid was subjected to GC-MS / FID analysis in the same manner as in Example 101.

[Comparative Example 102]
<Red Hoppe / Indoor / DHS Method>
The aroma collecting method of Comparative Example 102 was carried out in the same manner as in Example 101 except that the humidified body generating portion was not used and the air outside the system that was not humidified was supplied.
-Flow rate: Blower (unhumidified outside air; temperature 20 ° C, humidity 42%) 1.5 L / min, exhaust (suction) 1.5 L / min.
・ Collection time: 4 hours ・ Room temperature (outside the system): 20 ° C
・ Humidity (outside the system): 42%
Desorption and concentration from the adsorbent were carried out in the same manner as in Example 101 to obtain 43.0 mg (including solvent) of the aromatic liquid (fragrance concentrate) of Comparative Example 102. The obtained fragrance liquid was subjected to GC-MS / FID analysis in the same manner as in Example 101.

[evaluation]
<Sensory evaluation>
The samples (red hoppe) used as raw materials for animals and plants, and the aromatic liquids (fragrance concentrates) obtained in Example 101, Comparative Example 101, and Comparative Example 102 were subjected to sensory evaluation by 10 well-trained professional panelists. The results of the average sensory evaluation are shown in Table 3 below.

From Table 3 above, comparing the fragrance liquids (fragrance concentrates) obtained in Example 101, Comparative Examples 101 and 102, the fragrance liquids (fragrance concentrates) obtained in Example 101 have a good scent balance. , It was evaluated as the closest to the scent drifting from animal and plant raw materials (that is, a scent with a natural feeling). The fragrance liquid (fragrance concentrate) obtained in Comparative Example 101 had a weak scent and was conspicuous in sourness and sweetness. The fragrance liquid (fragrance concentrate) obtained in Comparative Example 102 had a green scent that was not noticeable in the scent of the sample.

<Aroma component>
(Abundance ratio of each component; GC-Area%)
Table 4 below shows the GC-MS / FID analysis results (compound name of the detected component) and the abundance ratio of each component in the aromatic liquids (fragrance concentrates) of Example 101, Comparative Example 101 and Comparative Example 102. The gas chromatogram obtained by GC-MS / FID analysis of the fragrance liquid (fragrance concentrate) obtained in Example 101, Comparative Example 101 and Comparative Example 102 is shown in FIG.

From Table 4 above, comparing the improved method of JP-A-2000-53992 of Example 101 with the aqua space method (conventional method) of Comparative Example 101, methyl butyrate (methyl butanoate), ethyl butyrate (ethyl butanoate), and ethyl butyrate are compared. Fruity esters such as ethyl pentanoate were in higher proportions in Example 101. Further, the cheese-like aroma of S-methyl thiobutanethioate was 0.11 GC-Area% in Example 101 and 0.02 GC-Area% in Comparative Example 101, showing a large difference. In addition, furaneol and γ-decalactone, which are considered to contribute to sweetness, were detected in a higher proportion in Example 101. On the contrary, examples of the components detected at a higher ratio in Comparative Example 101 include acids such as acetic acid and 2-ethylhexanoic acid, and methoxyfuraneol. It is presumed that the acids contribute to the sourness in the sensory evaluation of Comparative Example 101, and the methoxyfuraneol contributes to the sweetness.
Comparing the improved method of JP-A-2000-53992 of Example 101 with the DHS method of Comparative Example 102, a plurality of esters were detected at a higher rate in Comparative Example 102, and these were detected in the sensory evaluation of Comparative Example 102. It is presumed that it contributes to the fruity aroma with a sense of ripeness. Hexanal, acetic acid (Z) -3-hexenyl ([(Z) -hex-3-enyl] rate) and acetic acid (Z) -2-hexenyl ([(Z) -hex-2-enyl]). Since green aroma components such as acetate) were detected at a higher rate in Comparative Example 102, it is presumed that these contribute to the green feeling in the sensory evaluation of Comparative Example 102.

<Summary of strawberry evaluation results>
Regarding the scent collection method for strawberries (red cheeks), by using the scent collection system and the scent collection method of the present invention, an fragrance liquid (that is, a natural feeling) that is close to the scent (that is, has a natural feeling) drifting from real strawberries (aroma concentration). It turned out that I could get a thing).
From the above evaluation results, regarding the strawberry aroma collection method, it is considered that the aroma collection method of the present invention carried out in Example 101, that is, the aroma collection method by the improved method of JP-A-2000-53992 is effective. Conceivable.
For fruits and aromas such as strawberries (red cheeks) whose surface is dried and the aroma drifts from the surface and whose flavor is inferior when eaten, the aroma collecting method of the present invention (Japanese Patent Laid-Open No. 2000-53992). It is presumed that by using the improved method), the original aromas of animals and plants can be collected more efficiently, and an aromatic liquid with a natural feeling can be obtained. Compared with the aqua space method (conventional method), by using the aroma collection method of the present invention, esters, alcohols, lactones, etc., which greatly contribute to the aroma of fruits among volatile components, can be efficiently and originally obtained. It can be collected with a balance close to the aroma. On the other hand, in the non-humidifying DHS method, the green aroma presumed to be generated by drying stress is larger than the original aroma, but in the aroma collecting method of the present invention, the green aroma is less and the aroma has a more realistic balance. Can be collected.

In addition, when the aroma collection method (improved method of JP-A-2000-53992) and the DHS method of the present invention are applied to strawberries (red cheeks) before being peeled off in the house and field of a producer, Example 101 and Results with the same tendency as in Comparative Example 102 can be obtained.

[Example 201]
<Improved method of basil / indoor / Japanese Patent Application Laid-Open No. 2000-53992 (method for collecting aroma of the present invention)>
The aroma collection system used in Example 1 was modified as follows to be the aroma collection system of Example 201, and the aroma collection method of the present invention was carried out using the aroma collection system of Example 201. ..
As a raw material for animals and plants, basil (sweet basil), which is an herb grown in a soft plastic pot (pot seedling), was used.
Only the above-ground part of one basil strain is wrapped in a flexible bag (smart bag PA) and stored, with the bag mouth lightly in contact with the stem (without applying too much pressure to the basil), without sealing. Fixed. The air outside the system was supplied to the humidifying body generating part by a blower to obtain humidified air. The obtained humidified air is introduced into the gas inlet of the flexible bag (located on the vertically upper surface of the flexible bag), and the headspace gas of the flexible bag is discharged to the gas outlet (lower part of the flexible bag). (Placed on the side of the bag and vertically above the bag mouth), it was sucked out by a blower through the aroma component collecting part. By this step, humidified air passes mainly from the gas inlet of the flexible bag toward the gas outlet, and the aroma component is collected by the adsorbent. No cooling trap was used. Other conditions are as follows.
-Flow rate: blower (humidifier) 2.5 L / min, exhaust (suction); 2.0 L / min.
・ Collection time: By tapping the flexible bag from the outside for 1 minute every 6 hours and 30 minutes, the basil leaves are stimulated through the flexible bag and the aroma is released from the basil leaves. I let you.
The fragrance liquid (fragrance concentrate) of Example 201 was obtained by desorbing and concentrating from the adsorbent in the same manner as in Example 1. The obtained fragrance liquid was subjected to GC-MS / FID analysis in the same manner as in Example 1.

[Comparative Example 201]
<Basil / indoor / aqua space method (conventional method)>
Using the same basil as in Example 201 as an animal and plant raw material, aqua space collection was performed by the aqua space method (conventional method) using the same apparatus shown in FIG. 1 of JP-A-2000-53992 as in Comparative Example 2 indoors. rice field. A glass container was used as an animal and plant sample storage unit. The entire pot seedlings (including pots) of one Basil strain are stored in a glass container, and headspace gas is sucked in while introducing humidified air using two pumps under the following conditions, and a cooling trap (cooling trap (including pots)). Aroma components were collected by a trap (a trap cooled with dry ice in ethanol) to obtain a condensate (5.3 g).
-Flow rate: Blower (humidifier) 2.0 L / min, exhaust (suction) 2.0 L / min.
-Collection time: 6 hours After collecting the condensed liquid (5.3 g), all the walls of the cooling trap were washed with 20 mL of diethyl ether, and the entire amount of the obtained liquid was mixed with the condensed liquid. After adding 0.8 g of sodium chloride to the obtained mixed solution and dissolving it, the solution was separated and the aqueous layer was extracted twice with 5 mL of ether. After the ether layers were combined and dehydrated with Glauber's salt, the mixture was filtered through filter paper and concentrated at 43 ° C. under normal pressure to obtain the aromatic liquid (fragrance concentrate) of Comparative Example 201. The obtained fragrance liquid was subjected to GC-MS / FID analysis in the same manner as in Examples 1 and 201.

[Comparative Example 202]
<Basil / indoor / DHS method>
The aroma collecting method of Comparative Example 102 was carried out in the same manner as in Example 201 except that the humidified body generating portion was not used and the air outside the system that was not humidified was supplied.
-Flow rate: Blower (non-humidified air outside the system) 2.5 L / min, exhaust (suction) 2.0 L / min.
・ Collection time: By tapping the flexible bag from the outside for 1 minute every 6 hours and 30 minutes, the basil leaves are stimulated through the flexible bag and the aroma is released from the basil leaves. I let you.
The adsorbent was desorbed and concentrated in the same manner as in Examples 1 and 201 to obtain the aromatic liquid (fragrance concentrate) of Comparative Example 202. The obtained fragrance liquid was subjected to GC-MS / FID analysis in the same manner as in Examples 1 and 201.

[evaluation]
<Sensory evaluation>
The aromatic liquids (fragrance concentrates) of Example 201, Comparative Example 201 and Comparative Example 202 were subjected to sensory evaluation by 10 well-trained professional panelists.
The fragrance liquid obtained by the aqua space method (conventional method) of Comparative Example 201 was almost odorless. It is presumed that the aroma of basil was not emitted because the aroma collecting method of Comparative Example 201 could not sufficiently stimulate basil.
Comparing the fragrance liquids obtained by the improved method of JP-A-2000-53992 of Example 201 and the DHS method of Comparative Example 202, Example 201 has a fresher and more gorgeous impression than Comparative Example 202. The aroma was closer to that of the real basil leaves when stimulated.

<Aroma component>
As a result of GC-MS / FID analysis of the aromatic liquid (fragrance concentrate) of Comparative Example 201, the aroma yield was not sufficiently obtained, so that the analysis was difficult.
The abundance ratio (GC-Area%) of the main aroma components detected as a result of GC-MS / FID analysis of the aromatic liquid (aroma concentrate) of Example 201 and Comparative Example 202 is shown in Table 5 below. "Trace" in the table means a trace amount.

From Table 5 above, it was found that in Example 201, fresh floral linalool was collected at a higher rate than that of Comparative Example 202. Further, in Example 201, fresh green (Z) -3-hexenol ((Z) -3-hexenol), which was not detected in Comparative Example 202, and floral nerol and geraniol were collected. rice field. It is presumed that these contribute to the fresh and gorgeous basil-like aroma in the sensory evaluation of Example 201.

<Summary of evaluation results of basil>
Regarding the aroma collection method of basil (sweet basil), by using the aroma collection system and the aroma collection method of the present invention, a fragrance close to the aroma (that is, having a natural feeling) drifts when stimulating real basil. It was found that a liquid (aroma concentrate) could be obtained.
From the above evaluation results, it is considered that the aroma collecting method of the present invention carried out in Example 201, that is, the aroma collecting method by the improved method of JP-A-2000-53992, is effective as the method of collecting aroma of basil. Conceivable.

1 Blower 2 Moisture generator 3 Animal and plant raw material storage 4 Animal and plant raw material 5 Aroma component collector 6 Adsorbent 7 Water 8 Blower 9 Cleaner 10 Unit 11 Gas 12 Moisturizer 21 Fixing member 31 Gas inlet 32 Bag mouth Part 33 Gas outlet

Claims (16)

It has a humidifying body generating part, an animal and plant raw material storage part, and an aroma component collecting part in this order.
The animal and plant raw material storage section communicates with the humidifying body generating section and the aroma component collecting section.
The humidifying body generating portion generates a humidifying body which is humidified air or an inert gas, and causes the humidifying body to be generated.
An aroma collection system in which the aroma component collecting unit contains an adsorbent. The aroma collecting system according to claim 1, wherein the temperature of the aroma component collecting portion exceeds 0 ° C. The aroma collection system according to claim 1 or 2, wherein the adsorbent is a hydrophobic adsorbent. The aroma collection system according to any one of claims 1 to 3, wherein the animal and plant raw material storage portion is a flexible bag. A gas inlet through which the flexible bag communicates with the humidifying body generating portion,
A bag mouth that can be fixed in contact with at least a part of animal and plant raw materials, and
The aroma collection system according to claim 4, further comprising a gas discharge port communicating with the aroma component collecting unit. The aroma collection system according to any one of claims 1 to 5, wherein the humidity of the humidifying body is higher than the humidity outside the system at the place where the aroma collection system is installed. The stage where the humidifier generated in the humidifying body generation part is introduced into the animal and plant raw material storage part and brought into contact with the animal and plant raw material,
Including the step of introducing a gas containing the aroma of the animal and plant raw materials into the aroma component collecting section to collect the aroma component.
The humidified body is humidified air or an inert gas.
A method for collecting aroma, wherein the aroma component collecting portion contains an adsorbent. The step of preparing the humidifier by introducing air or an inert gas into the humidifier generator is included.
The aroma collecting method according to claim 7, wherein the step of preparing the humidifying body is a step of introducing the air or the inert gas into water. The stage of preparing the humidifier is the stage of introducing extra-system air into the humidifier generator, and
The aroma collecting method according to claim 8, wherein the humidity of the humidifying body is higher than the humidity outside the system. The method for collecting aroma according to any one of claims 7 to 9, wherein the animal and plant raw materials are at least a part of a growing plant. The animal and plant raw material storage part is a flexible bag.
The method for collecting aroma according to claim 10, further comprising a step of deforming the flexible bag to stimulate at least a part of the growing plant. The animal and plant raw materials are at least part of the plant and
The invention according to any one of claims 7 to 11, wherein at least a part of the plant is at least one of flowers, buds, vegetables, herbs, trees, bark, branches, leaves, buds, roots and fruits. How to collect aroma. The method for collecting aroma according to any one of claims 7 to 12, comprising a step of passing an organic solvent through the adsorbent to obtain an aromatic liquid containing the aroma component. A method for producing an aromatic liquid, comprising a step of obtaining an aromatic liquid by the aroma collection system according to any one of claims 1 to 6 or the aroma collection method according to any one of claims 7 to 13. The composition of the fragrance liquid is reconstructed based on the step of performing the component analysis of the fragrance liquid produced by the method for producing the fragrance liquid according to claim 14 and the component analysis result of the fragrance liquid obtained in the step. A method for producing an aromatic composition, which comprises the step of obtaining the aromatic composition. A method for producing a blended fragrance, which comprises a step of combining the fragrance composition produced by the method for producing a fragrance composition according to claim 15 with another fragrance to obtain a blended fragrance.

Images