CN102631409A

CN102631409A - Health-care medicine formula with functions of enhancing immunity, alleviating physical fatigue and resisting tumor

Info

Publication number: CN102631409A
Application number: CN2011103646820A
Authority: CN
Inventors: 李�杰; 施建耀
Original assignee: NINGBO HAIYI BIOTECHNOLOGY CO Ltd
Current assignee: NINGBO HAIYI BIOTECHNOLOGY CO Ltd
Priority date: 2011-11-17
Filing date: 2011-11-17
Publication date: 2012-08-15
Anticipated expiration: 2031-11-17
Also published as: CN102631409B

Abstract

The invention discloses a health-care medicine formula with functions of enhancing immunity, alleviating physical fatigue and resisting tumor, and relates to a health care medicine. The health-care medicine formula comprises the following components in parts by weight: 6-15g of broccoli extractive, 9-20g of Astragalus mongholicus, 9-20g of salviae miltiorrhizae, 6-12g of dangshen, 6-12g of resveratrol, 0.04-0.1g of curcumin, 0.004-0.01g of selenium carrageenan and 2-10g of microcrystalline cellulose. The health-care medicine formula has the following preparation technology: firstly, respectively smashing the Astragalus mongholicus, the salviae miltiorrhizae, the dangshen and the resveratrol medicines, and sieving by a sieve with 60-80 meshes; respectively taking the powder, mixing the powder with other raw materials and auxiliary materials for 45-50 minutes; filling in a capsule; in the filling process, controlling the filling weight strictly according to the internally controlled standard; then screening, polishing, internally packing, and externally packing; and finally, checking according to the inspection standard, and putting into storage. The health-care medicine can help people to enhance the immunity, alleviating the physical fatigue, resisting the tumor, improving the immunity of the human body and improving the life quality of the patients. The health-care medicine formula does not have side effects or adverse reaction. Due to the adoption of the modern preparation technology and the advanced quality control method, the product is safe and stable and has controllable quality.

Description

The health medicine prescription of a kind of enhancing immunity, alleviating physical fatigue, anti-tumor function

Technical field:

The present invention relates to the health medicine prescription of a kind of enhancing immunity, alleviating physical fatigue, anti-tumor function.

Background technology:

These article are suitable for the extensive crowd of hypoimmunity.Global the survey showed that according to one of World Health Organization (WHO), and the people of whole world true health only accounts for 5%, through doctor's inspection, diagnoses ill people also only to account for 20%, 75% people and then be in sub-health state.In May, 2002, the crowd that CPMA inference China is in sub-health state is 70% (about 800,000,000), and most people's hypoimmunity.

" in healthy energy deposited, heresy can not be done, and the institute of heresy gathers, and its gas must be empty ".Mean: when having very strong resistance in the human body, disease is impossible invade health, the disease place of arrival, and its resistance is weak certainly.The basic reason that the people falls ill is that resistance (medically claiming immunity) is low.Modern medicine study proves, eliminates subhealth state, prevent disease, and the most effectively way is exactly the immunity of enhances human body to disease.Therefore develop the effect uniqueness, have no side effect, the health product with function of enhancing immunity of high in technological content, pure natural, necessarily have vast market prospect and good social benefit.

According to market survey, though it is many to have the health product of identical function in the market, market demand is still huge, in addition with the Caulis et Folium Brassicae capitatae nutritious, that effect is unique be the primary raw material development product but seldom, so market remains exploitation.These article have following characteristics: this formula for a product rationally, definite ingredients, effect be reliable; Take safe, without any side effects; Easy to carry, be convenient to preserve; Process technology is advanced, quality guaranteed (these article production and processing overall process is carried out in strict accordance with the GMP standard).Caulis et Folium Brassicae capitatae in the prescription is the sharpest edges of these article; The contained nutrient substance of Caulis et Folium Brassicae capitatae is very abundant; Not only contain protein, sugar, fat, mineral, vitamin C and carotene etc., glucosinolate content is very abundant especially, is main with Radix Raphani sulfur glycosides (4-methylsulfinyl cyclobutenyl thioglycoside); Its catabolite sulforaphen is the strongest active component of finding in the vegetable up to now of anticancer vigor; Have the obvious microenvironment of improving human body cell, can improve body immunity, promote the effect of death of neoplastic cells.Therefore than other products, the health-care effect of this product is even better.

China is engaged in the research and the production of natural drug; Advantageous condition is arranged; The rarity of Traditional Chinese Medicine, it has clinical experience in several thousand, from present pharmaceutical research trend; From natural product, seek new drug or find that the guide is combined to thing and it is carried out the important content that structural modification is a drug development, along with the People more and more that day by day seriously makes of social environmental pollution is liked pure natural, pollution-free, the food that do not add synthetics.Adding growth in the living standard also makes people that the quality of life has been had higher pursuit.Under this background, this prescription utilization high-tech means and the hypoglycemia healthcare food that is raw material with free of contamination pure natural animals and plants arise at the historic moment, and develop rapidly.

Summary of the invention:

The health medicine prescription that the purpose of this invention is to provide a kind of enhancing immunity, alleviating physical fatigue, anti-tumor function; Its spy, it can strengthen people's immunity, alleviating physical fatigue, assist people antitumor, improves people's living standard; Have no side effect and untoward reaction; And use modern preparation technique, advanced method of quality control makes product accomplish safety, stable, quality controllable.

In order to solve the existing problem of background technology, the present invention adopts following technical scheme: the prescription ratio of medicine is: Caulis et Folium Brassicae capitatae 6-15g, Radix Astragali 9-20g, Radix Salviae Miltiorrhizae 9-20g, Radix Codonopsis 6-12g, resveratrol 6-12g, curcumin 0.04-0.1g, selenocarrageenan 0.004-0.01g microcrystalline Cellulose 2-10g.

The optimization formula ratio of this medicine is Caulis et Folium Brassicae capitatae 6g, Radix Astragali 9g, Radix Salviae Miltiorrhizae 9g, Radix Codonopsis 6g, resveratrol 6g, curcumin 0.04g, selenocarrageenan 0.004g microcrystalline Cellulose 2g.

Another one optimization formula ratio is Caulis et Folium Brassicae capitatae 15g, Radix Astragali 20g, Radix Salviae Miltiorrhizae 20g, Radix Codonopsis 6-12g, resveratrol 12g, curcumin 0.1g, selenocarrageenan 0.01g microcrystalline Cellulose 10g.

Preferred forms is that the pharmaceutical formulation ratio is Caulis et Folium Brassicae capitatae 11g, Radix Astragali 16g, Radix Salviae Miltiorrhizae 14g, Radix Codonopsis 9g, resveratrol 10g, curcumin 0.08g, selenocarrageenan 0.006g microcrystalline Cellulose 8g.

Through experiment showed, that this medicine adopts aforementioned proportion, to the inhibition of cancerous cell, especially the inhibition of HCC has obvious effect, and the normal growth for guaranteeing the cell body prevents cell carcinogenesis, has positive effect.

Its method for preparing is: respectively the Radix Astragali, Radix Salviae Miltiorrhizae, Radix Codonopsis, resveratrol medicine are pulverized earlier; Cross the 60-80 mesh sieve, mix incorporation time control 45-50 minute after getting its powder and other supplementary material weighing respectively; Carrying out capsule again fills; Filling process is controlled filling weight in strict accordance with inner quality standard, screens then, polishing, inner packing, outer package, at last according to touchstone check warehouse-in;

After experiment showed, that this manufacture method of medicine mixes 45-50 minute before capsule is filled, has tangible drug effect raising effect.

The present invention has following beneficial effect: can enhancing immunity, alleviating physical fatigue, anti-tumor function; Improve people's health level, have no side effect and untoward reaction, and use modern preparation technique; Advanced method of quality control makes product accomplish safety, stable, quality controllable.

The specific embodiment:

This specific embodiment adopts following technical scheme: its prescription is than being Caulis et Folium Brassicae capitatae 6-15g, Radix Astragali 9-20g, Radix Salviae Miltiorrhizae 9-20g, Radix Codonopsis 6-12g, resveratrol 6-12g, curcumin 0.04-0.1g, selenocarrageenan 0.004-0.01g microcrystalline Cellulose 2-10g.Its preparation technology is: respectively the Radix Astragali, Radix Salviae Miltiorrhizae, Radix Codonopsis, resveratrol medicine are pulverized earlier; Cross the 60-80 mesh sieve, mix incorporation time control 45-50 minute after getting its powder and other supplementary material weighing respectively; Carrying out capsule again fills; Filling process is controlled filling weight in strict accordance with inner quality standard, screens then, polishing, inner packing, outer package, at last according to touchstone check warehouse-in.

Caulis et Folium Brassicae capitatae:

Caulis et Folium Brassicae capitatae raw material in the prescription is processed through alcohol extraction process with our common vegetable Caulis et Folium Brassicae capitatae used in everyday.Caulis et Folium Brassicae capitatae meets " Ministry of Public Health is about the notice of further standard healthy food material management " requirement as the bread and cheese raw material; Caulis et Folium Brassicae capitatae is with its nutrient substance that is rich in and the unique effects that has; Along with progressively going deep into of scientific research; More and more receive people's attention, and beginning has obtained exploitation faster and has used at field of food.Caulis et Folium Brassicae capitatae not only contains protein, sugar, fat, mineral, vitamin C and carotene etc.; Glucosinolate content is very abundant especially; With Radix Raphani sulfur glycosides (4-methylsulfinyl cyclobutenyl thioglycoside) is main, and its catabolite sulforaphen is the strongest active component of finding in the vegetable up to now of anticancer vigor, has the obvious microenvironment of improving human body cell; Can improve body immunity, promote the effect of death of neoplastic cells.

External relevant bibliographical information Caulis et Folium Brassicae capitatae have function of enhancing immunity; P.Thejass for example; Experimental result shows in " probe into the immunocompetence of sulforaphen, a kind of from Brassica oleracea L. var. botrytis L. the isothiocyanate of natural extract " literary composition that G.Kuttan delivers on " plant amedica (magazine) ": sulforaphen has the effect of the peritoneal macrophage that improves activate the phagocytic capacity.The effect of sulforaphen also can significantly reduce the tumor necrosis factor that is increasing in addition--and-a lipopolysaccharide stimulates the macrophage that produces.These results show that sulforaphen has the immunocompetent effect of adjusting. According to Thejass P, Kuttan G is in that " " isothiocyanate that sulforaphen is a kind of to derive from the nature of Caulis et Folium Brassicae capitatae increases the cytotoxicity that BALB/c mouse NK cell and antibody rely on through strengthening interleukin II and tumor necrosis factor " literary composition of delivering on immune pharmacology and the immune toxicity journal is reported: sulforaphen is to normally and the research of the influence of the cell-mediated immunity of the BALB/c mouse of Ehrlich ascites inoculated tumour.Sulforaphen significantly increases the NK cytoactive of normal and inoculated tumour animal.NK cytoactive among the intact animal is observed than inoculated tumour cell animal is more Zao.The cytotoxicity that the antibody of the animal of the normal and inoculated tumour that sulforaphen is handled relies on is significantly strengthened than the animal of the inoculated tumour that does not have sulforaphen to handle.In the animal of the normal and inoculated tumour that sulforaphen is handled, observe a kind of cytotoxicity of complementation mediation of early antibody dependence.Sulforaphen is handled interleukin II and tumor necrosis factor-gamma in the animal that has significantly increased normal and inoculated tumour.Sulforaphen is handled and has significantly been promoted splenocyte, medullary cell, thymocyte proliferation in addition.Show that sulforaphen has the effect of raise immunity; According to Thejass P, Kuttan G " deliver on immune pharmacology and the immune toxicity journal " sulforaphen is to B16F-10 MelanomaInductive MigrationThe property tumor inoculation the adjusting of response of C57BL/6 mouse cell mediation immunity " a literary composition report: sulforaphen is handled and has significantly been strengthened MigrationThe NK cytoactive of property tumor inoculation animal has significantly increased inoculation MelanomaAnimal in interleukin II and tumor necrosis factor; Sulforaphen has significantly reduced the toxic level of proinflammatory cytokine in the serum during neoplasm metastasis in addition; Through the response of sulforaphen irritation cell mediation immunity, raise IL-2 and IFN-gamma, downward modulation proinflammatory cytokine toxicity IL-1beta; IL-6, TNF-alpha and GM-CSF.

Domestic relevant bibliographical information Caulis et Folium Brassicae capitatae have function of enhancing immunity; Show in " sulforaphen extraction, separation and Anticancer Activities in the Caulis et Folium Brassicae capitatae " literary composition that for example refined, the Chi Wenjie of season word, Zou Xiang, Wang Zhiju deliver that sulforaphen has tangible blocking effect especially obvious to hepatocarcinoma, the esophageal carcinoma, preceding gastric cancer effect to rodent hepatocarcinoma, breast carcinoma, pulmonary carcinoma, the esophageal carcinoma, preceding gastric cancer on " Harbin University of Commerce's journal " (changing the science version over to); Experiment proof they can Phase II (cancerigenic factor detoxifcation) enzyme generation; Thereby play antitumaous effect; These enzymes can be through the active center of destroying cancerigenic factor or the method that they and endogenous aglucon are combined, and acceleration excretes it.Data proves that sulforaphen is through carcinogen metabolic regulation mechanism is worked.The research worker of vast this John Hopkins University of the U.S. is utilized transgenic mouse experiment proof: a kind of system of ubiquity in many animal bodies, the carcinogenesis that is used for resisting toxic chemical substance in food and the environment.The performance of this effect depends on the remarkable increase of Phase II enzyme quantity.This enzyme can be eliminated harmful chemical, suppresses its infringement DNA, thereby reaches anticancer disease effect.Two sulforaphen are the most intensive Phase II enzyme inducers of finding up to now; Deliver in " purification and the anti-tumor activity of main sulfur glycosides in the Semen Brassicae campestris " literary composition in " applied chemistry " (the 22nd the 10th phase of volume) according to the Zhou Jinlan of department of chemistry of the Central China University of Science and Technology, Yu Kaichao and to show: the sulfur glycosides itself is kind of a more stable chemical compound, but can be become multiple products such as isothiocyanate (ITC), oxazolidine thione (OZT) and nitrile compounds by bacterial enzyme catalytic degradation in myrosase or the gastrointestinal tract.The antibacterial action that these catabolites have stronger inhibition fungus and microbial reproduction etc. reaches through inducing the carcinogenesis of the active blocking-up of ubiquinone reductase carcinogen; According to first middle school, Qinhuangdao, Hebei province, Hebei Normal University chemistry and material science institute Shen tree virtue, Zhang Yingfeng, nightstool river " chemistry teaching " (2009; The 7th phase) shows in " the anticancer pharmacology of thioglycoside in the Caulis et Folium Brassicae capitatae " literary composition of delivering on that Radix Raphani sulfur glycosides can induce body to produce the II type and separate toxenzyme-glutathione transferring enzyme and quinone reductase; This enzyme can produce resistance to many carcinogens, thereby plays antitumaous effect; According to the Beijing University of Technology engineering constraint learn, that The Hong Kong Polytechnic University's applying biological and chemical science and technology is Wang Jiandong, Yuan Qipeng, Qian Zhongming points out that sulforaphen acts on the body process in " sulforaphen progress " literary composition that " food and fermentation industries " delivered is following: (1) enzyme II gene transcription.Transcribe the II type enzyme that only produces the human body beneficial, inhibitory enzyme I produces simultaneously, and enzyme I can produce cooperative effect with carcinogen, quickens cancer and takes place.(2) enzyme II acts on the anticancer system of body self.Through this system, can realize following regulation and control: 1. block carcinogen and produce the required metabolic pathway of carcinogenic effect, stop carcinogen or its metabolite to get into target cell, play the carcinogenic effect of removing simultaneously to metabolism; The division of anticancer and growth, blocking-up circulation approach; But the red carcinogen of the protein secreting ancestral who promotes other kill cancer cell is induced the effect of cancer.2. the hydrolyzate of the sinigrin startup generation effect that possibly play a role to carcinogen; At the cancer formation initial stage gene is undergone mutation; Also have some inhibitory enzymes to have dual resistance cancer or cancer suppressing function in addition, Toxic is degraded, become and press down the cancer approach thereby play anticancer effect one; According to Zhejiang Prov Industrial And Commercial University's food is that Wang Zhongying, Wang Xiangyang show in " thioglycoside and catabolite isothiocyanate thereof " literary composition that " chemistry of life " (rolling up for 4 phases in 2005 25) delivered: the anticancer mechanism of isothiocyanate comprises two parts: (1) is through suppressing the process that the Phase I enzyme stops (comprising cytochrome p450 system) chemocarcinogenesis; Particularly PEITC and BITC can effectively suppress cancerigenic factor-DNA and add the formation with thing; Thereby suppress forming by the inductive tumor of cancerigenic factor; (2) induce the generation Phase to separate toxenzyme; For example glutathione transferase and UDP-glucuronyl transferase etc., thus the overactive carcinogenic intermediate product that cytochrome p450 system produces reduced.Wherein SUL induces and produces Phase to separate the ability of toxenzyme strong especially, is the natural inducer that the most effectively Phase that identifies is up to now separated toxenzyme.Though human body is had certain toxic action for the SUL of high concentration and fragrant alkane isothiocyanate but they (in 0.05～20umol), do not show 200～400 milligrams of the oral suggestions of toxicity human body, can effectively prevent multiple cancer in valid density.

With the Caulis et Folium Brassicae capitatae consumption in this prescription, according to hide naval, Zhang Keying shows in " harm of thioglycoside and detoxification Study on Measures in the dregs of rapeseed cake " literary composition that " feed industry " (2007 the 28th volumes) delivered: pig, rabbit, fowl, fish and ruminant to daily ration in the tolerance level of thioglycoside be respectively 0.78,7.0,5.4,3.6 and 1.5～4.22umol/g; Show in " applied chemistry " (the 20th the 10th phase of volume) " purification and the anti-tumor activity of main sulfur glycosides in the Semen Brassicae campestris " literary composition,, mice sulfur glycosides least action dosage 30mg/kg shows that the sulfur glycosides has obvious inhibitory action to S180 tumor in the mice body; Show in " thioglycoside and catabolite isothiocyanate thereof " literary composition that deliver " chemistry of life " (2005 25 volume 4 phases): though the SUL of high concentration and fragrant alkane isothiocyanate to human body have certain toxic action they in valid density (in 0.05～20umol); Do not show 200～400 milligrams of the oral suggestions of toxicity human body, can effectively prevent multiple cancer.According to above different documents and materials, be test raw material with Radix Raphani sulfur glycosides in this prescription Caulis et Folium Brassicae capitatae raw material, experimentize by adult (50kg weighing machine) 34mg every day.

1. 30 times, 10 times of human body recommended amounts are established in ear swelling method experiment, calculate according to formula: promptly every day, Radix Raphani sulfur glycosides 22.48mg/kg, 8.16mg/kg were respectively high low dose group.Actual disposition needs the high low dose group 27.98mg/kg of sample Radix Raphani sulfur glycosides, 9.33mg/kg, dosage 0.4ml/20g Mus weight/d when irritating gastric juice.Every day gastric infusion, experimental result is seen table 1, table 2.

Table 1 Radix Raphani sulfur glycosides immunological enhancement: body weight organ index

Table 2 sulforaphen Radix Raphani sulfur glycosides immunological enhancement: ear swelling

Experimental result compare the high low dosage of Radix Raphani sulfur glycosides with blank control group to not obviously influence of body weight, but the ear swelling experiment shows that high low dose group all has tangible immunological enhancement.

2. humoral immunization serum hemolysin experiment, the high 1.2mg/ml of Radix Raphani sulfur glycosides; Radix Raphani sulfur glycosides hangs down 0.4mg/ml, dosage 0.4ml/20g/d, every day gastric infusion.The result sees table 3 table 4:

Table 3 sulforaphen Radix Raphani sulfur glycosides immunological enhancement: body weight organ index

Table 4 sulforaphen Radix Raphani sulfur glycosides immunological enhancement: serum hemolysin

Experimental result is compared with blank control group, and the positive drug Thymosin alpha 1 is influential to body weight, and the determination experiment of serum hemolysin shows its obviously enhancing body humoral immunization.All the other each groups compare with normal group, and body weight and organ index are not all had remarkable effect.The serum hemolysin and the antibody product of the high low dosage of Radix Raphani sulfur glycosides have raising, p＜0.05, and low metering group also improves with respect to blank control group.The determination experiment of serum hemolysin shows that just metering group of Radix Raphani sulfur glycosides has certain enhancing immunity effect.

The Radix Astragali

Radix Astragali head is stated from Shennong's Herbal, sweet in the mouth, and slightly warm in nature is returned the spleen lung meridian, has the effect that same table, diuresis, promoting pus discharge and tissue regeneration strengthening are defended in spleen invigorating invigorating middle warmer, ascending up spleen-Qi and Yang, benefit.Modern study shows that the Radix Astragali contains materials such as multiple glycoside, polysaccharide, flavone, folic acid and various trace elements, has antitumor, regulates pharmacological actions such as immunity, antiviral, defying age, antioxidation, radioprotective and anti-stress.

1, the ``1 such as antitumor action of the Radix Astragali discover, compare Ramulus et folium taxi cuspidatae with independent use paclitaxel treatment

1.1 human body immunity improving function stone Ren Bing etc. find in to the research of chemotherapy tumor-bearing mice immune function influence and tumor-inhibiting action at Radix Astragali S4; List all is higher than list with cyclophosphamide group and negative control group with thymus index, the index and spleen index that the Radix Astragali group and the Radix Astragali add cyclophosphamide group tumor-bearing mice, and difference has statistical significance; The proof Radix Astragali has protective effect to immune organ, and chemotherapy damage immunologic function is had better protect and promotes restitution.Tumor-bearing mice after the cyclophosphamide chemotherapy of the yellow late-maturing treatment of application such as Ying Zizhong immunocompromised; Find that IgM, IgG significantly increase in the tumor-bearing mice body; Explain that the Radix Astragali can significantly promote the thymus humoral response in anti-tumor immunotherapy, improve the specific immune response of host cancerous cell through stimulating the huge system of biting of monokaryon.Li Peiwen etc. find that also Radix Astragali injection can improve the tumor patient immunologic function, strengthens its humoral immunization and cellular immune function.

1.2 inhibition tumor cell proliferation; Research astragalus root components such as promotion apoptosis of tumor cells Zhao Lian China are in the inhibitory action of novel formulation to people CCL-229, K562 cancerous cell; Find that astragalus root components F3 novel formulation has the obvious suppression effect to 2 kinds of cancerous cell; And be the concentration dependence, more remarkable with cisplatin combined medication effect; The shrinkage of cancerous cell peplos, kytoplasm after the visible Radix Astragali effect of transmission electron microscope observing assembled, chromatin concentrates, and visible karyorrhexis and apoptotic body form.Liu Hui etc. " to discover; after the Radix Astragali acted on the transplanted tumor of lotus people squamous cell carcinoma strain GNM tumor bearing nude mice, mirror was observed down and is seen that the cancerous cell form is irregular, cavity is arranged in the endochylema; the part cell has the karyorrhexis phenomenon; and see the liquefaction and necrosis district, explain that the Radix Astragali has anticancer propagation, promotes its effect of apoptosis.

1.3 the influence heavy to tumor

The result shows that the tumor weight average of the test group of various dose is lower than negative control group (P＜0.05), and LL increases along with Radix Astragali dosage, and tumour inhibiting rate has the trend that increases gradually, and the tumor killing effect tool that shows the Radix Astragali is from. dose response fire system.

The influence that table 5 Radix Astragali is heavy to mice S180 tumor

Annotate: * representes to compare P＜0.05 with negative control group

1.4 to immune influence

The result shows that the T lymphproliferation response of positive controls and NK cytotoxic activity all are lower than negative control group (P＜0.05), shows that cyclophosphamide has certain damage to immune system; Use the T lymphproliferation response and the NK cytotoxic activity of three experiment knobs of the Radix Astragali all to be higher than negative control group (P＜0.05); And increase along with dosage; Above-mentioned two indexes has the trend that increases gradually, shows that the Radix Astragali can strengthen immune function of mice, and dose-response relationship is arranged.

Table 6 Radix Astragali is to immune influence

Annotate: compare P＜0.05 between two dose groups in twos; Compare * P＜0.05 with negative control group

The Radix Astragali is a kind of effective biological regulator, and the research of rice in recent years shows that the Radix Astragali has great potential at the control towel of tumor.This research is successfully being duplicated on the mouse-borne tumor model based; Adopt the astragalus polysaccharides of various dose to intervene; The tumor weight average was lower than negative control group (P＜0.05) when the mice that the result shows all experimental grouies was finished in experiment, and along with the increase of dosage, tumor heavily has the trend (P＜0.05) that reduces gradually; Show that astragalus polysaccharides has tumor-inhibiting action, and there is dose-effect relationship in this effect.Under normal circumstances, be in poised state between the defence of tumor and body, the generation of tumor and even propagation and send out are due to the imbalance of this dynamic equilibrium fully.If will be imbalance state be adjusted to normal level artificially, may command tumor growth even it is disappeared then.Its host immune function of tumor patient is disequilibrium; The immunoreation of advanced tumor body almost disappears, and traditional chemotherapeutics especially selectively kills and wounds normal cell in the kill tumor cell; Particularly the hemopoietic tissue cell usually causes body generation severe complication.This result of study shows that the Radix Astragali can improve the lymphproliferation response that leans on the tumor mice, also can improve the NK cell activity, explains that the Radix Astragali suppresses tumor through human body immunity improving power.

Radix Salviae Miltiorrhizae

Radix Salviae Miltiorrhizae is recorded in Shennong's Herbal the earliest, in the book on Chinese herbal medicine in successive dynasties, has more and records.Radix Salviae Miltiorrhizae, bitter in the mouth, cold nature, GUIXIN, pericardium, Liver Channel.Effect is that blood circulation promoting and blood stasis dispelling, removing heat from blood eliminating carbuncle, relieving restlessness are calmed the nerves.The thirties in 20th century, just the begin one's study chemical constituent of Radix Salviae Miltiorrhizae of Chinese scholars, and successively from Radix Salviae Miltiorrhizae, separate obtained 40 surplus kind of water soluble compound surplus kind of the fat-soluble compound and 10, some chemical constituents wherein are material bases of Radix Salviae Miltiorrhizae performance clinical efficacy.Obtained very to the research of Radix Salviae Miltiorrhizae that plentiful and substantial pharmacological effect effect shows in recent years, the extract of single medicinal material Radix Salviae Miltiorrhizae or ginseng or Radix Salviae Miltiorrhizae have certain antitumor action with the compound preparation of other drug composition.Therefore, Radix Salviae Miltiorrhizae and extract thereof have clinical preferably antitumor application prospect.

1, suppresses tumor cell proliferation

Cell cycle regulating is not normal to be the main cause of tumor abnormality proliferation, and suppressing cell proliferation is a kind of approach of antineoplastic.The Radix Salviae Miltiorrhizae extract tanshinone can make S phase hepatocarcinoma SMMC-7721 cell proportion descend, G0 with (or) G1 phase ratio rises C-myc protein expression downward modulation simultaneously.The C-fos protein expression raises i.Zhong Zhihong has found that also tanshinone can make G0/G1 phase human hepatoma HepG2 cell ratio increase ii.In s, Tanshinone I Lk can make the processed group inhibitory rate of cell growth apparently higher than matched group, and its effect is concentration and time dependence iii.

2,, inducing apoptosis of tumour cell

Apoptosis is that multicellular organism is kept one of homeostatic important physiological mechanism, and apoptosis of tumor cells is unbalance with propagation to be the major reason that tumor forms and develops.Inducing apoptosis of tumour cell is another approach of antineoplastic.Experiment finds, act on hepatocarcinoma BEL-7402 cell with TANSHINONES 11A after, cell generation obvious apoptosis iv.The refined blue v of Yuan finds in the research of TANSHINONES to nasopharyngeal carcinoma cell CNE-1 cytosis; Processed group CNE-1 apoptosis is than the obvious increase of matched group; And short apoptosis-related genes Fas; Bax and P53 and cell cycle negative regulator gene P21 up-regulated, and press down apoptogene Bcl-2 down-regulated expression infer that thus the TANSHINONES inducing apoptosis of tumour cell maybe to press down apoptogene relevant with raising short gene participating in apoptosis and downward modulation.Tanshinone also can make HL-60 cell generation apoptosis, the hypodiploid apoptotic peak occurs, and c-fas increases, and c-myc and Bcl-2 reduce vi.

3, improve the tumor hypoxia state

Weary oxygen is the problem of people's growing interest in recent years.Quick growth along with entity tumor the low oxygen concentration district can occur in the tumor tissues.Weary oxygen inducible factor HIF-1a plays an important role at tumor hypoxia molecular signal transduction path.Weary oxygen increases the tumor autoinvasive, and resists chemicotherapy.Therefore, how changing the tumor hypoxia state, is another difficult problem in the antitumor research.Radix Salviae Miltiorrhizae has the effect of blood circulation promoting and blood stasis dispelling, can microcirculation improvement, reduce the effect of blood viscosity and blood vessel dilating, and can remove oxygen-derived free radicals.Theoretically, Radix Salviae Miltiorrhizae can improve the tissue anoxia state.Feng Yu light vii is at in-vitro simulated low-oxygen environment, and the research tanshinone is to the effect of gastric cancer SCG7901 cell, and the result finds that tanshinone can improve anoxia state, suppresses HIF-1a and c-myc and expresses.

4, make drug-resistant tumor cell enhanced sensitivity

The cell drug resistance is that normal cell is kept one of homeostatic defense mechanism, also is the main cause that causes tumor chemical therapy failure and tumor recurrence.Tumor cell is prone to multiple chemotherapeutics is produced cross resistance.This is the difficult problem of clinical cancer therapy.At present modal is the tumor multi-medicine drug-resistant phenomenon; Its generation mechanism is divided into classical transport of drug resistance mechanism and non-classical multidrug resistance mechanism x, and wherein to efflux that the apoptosis that causes with Bcl one 2 high expresseds suppresses be the common mechanism that multidrug resistance takes place to the P-gp medicine.Liu Changjian xi finds that the microemulsion Radix Salviae Miltiorrhizae can well reverse the multidrug resistance into leukemia amycin persister K562/ADM (KA), and the mdr cell apoptosis rate is increased, and P-gPBcl-2 expresses reduction.

5, respectively organize the comparison of rat implantation property hepatocarcinoma gross tumor volume, tumor propagation rate

Table 7 is respectively organized the variation (x ± s) of gross tumor volume and rate of increase before and after the rats'liver arterial perfusion

Compare * P＜0.05 with model group.**P＜0.01

6, respectively organize the variation of rats'liver function

Table 8 is respectively organized rats'liver arterial perfusion front and back ALT, AST changes

Research shows, uses Radix Salviae Miltiorrhizae hepatic arterial infusion rat implantation property liver cancer model, and hepatocarcinoma is had obvious therapeutic action, can effectively suppress the growth of gross tumor volume.Liver function injury is the more common toxicity of chemotherapy.This experiment shows that through 1 week of perfusion Radix Salviae Miltiorrhizae back result the degree of injury of Radix Salviae Miltiorrhizae group liver function is starkly lower than matched group and mitomycin group, and statistical procedures has significant difference, discloses the effect that Radix Salviae Miltiorrhizae has obvious reduction liver function injury.Prompting Radix Salviae Miltiorrhizae hepatic arterial infusion has the effect of excellent protection liver in treatment hepatocarcinoma.

Radix Codonopsis

Radix Codonopsis is a China tradition rare Chinese medicine, " Pharmacopoeia of People's Republic of China) 2005 editions Radix Codonopsis that record are the dry root of campanulaceae plant Radix Codonopsis, plain flower Radix Codonopsis or radix codonpsis tangshen, have invigorating the spleen and replenishing QI; Spleen invigorating cures mainly deficiency of the spleen and lung, the cardiopalmus of breathing hard, anorexia and loose stool; Dyspnea due to deficiency cough, interior-heat disease such as quench one's thirst.Discovery Radix Codonopsis water extracts such as Jia Taiyuan have tangible potentiation to the activate the phagocytic capacity of Mus J774 macrophage; And infer that this effect is the enhanced basis of immunity of organism. observations such as Koryo pine think that magnetic treatment Radix Codonopsis medicinal liquid has the effect that promotes the mononuclear phagocyte system phagocytic function, shows good action to immune system. Zhong Zhimin etc. detect Radix Codonopsis can promote PMNC CD ²⁺The growth of cell; The prompting Radix Codonopsis helps to improve discovery Radix Codonopsis such as immunologic function .B E Shan can stimulate lymphopoiesis; But act on faint; Observing Radix Codonopsis simultaneously Ig and IL-1 are had certain effect. Wang Min etc. find that in the mice aging model that causes with D one galactose Radix Codonopsis and compound recipe thereof can improve the IL-2 level of mice; Thereby raise immunity; Play function in delaying senility. observations such as Tang Qingxiu find that the Radix Codonopsis essence is produced in Gansu and Radix Codonopsis polysaccharide can improve the Turnover of Mouse Peritoneal Macrophages phagocytic function; And increase immune organ weight. Cao Li etc. and Li Yan etc. have inquired into the effect to normal spleen lymphocyte proliferation of Radix Codonopsis polysaccharide and codonopsis clematidea Clarke's polysaccharide respectively; All find to have facilitation; And the former acts on obviously. and Cao Li etc. find that also Radix Codonopsis polysaccharide has tangible promotion proliferation function equally to the lymphocyte that concanavalin A, Con A stimulates. and the antibody horizontal in the immunologic process also is an aspect of reaction immunoregulation effect. and experiments such as Yang Guang show that Radix Codonopsis polysaccharide generates normal mouse antibodies potentiation is arranged. Zhang Xiaojun etc. have reported that Radix Codonopsis polysaccharide has strong facilitation to humoral immunization, and the pair cell immunity has experiment discovery Radix Codonopsis polysaccharide such as facilitation .Z T Wang that immunosuppressive action is arranged when low dose of administration, but the pinocytosis process of macrophage is not had effect; It is active that discovery codonopsis clematidea Clarke polysaccharide such as Xiong Yuanjun can increase SOD in the body. and discovery Radix Codonopsis such as Huang Liya and injection thereof can raise the expression of (GSH-Px); The prompting Radix Codonopsis can be resisted oxygen free radical injury; Belonging to the strong anti-oxidation medicine also plays an important role in the defying age process. and Radix Codonopsis also has the effect of removing free radical. and the scavenging action to ultra-oxygen anion free radical of Radix Codonopsis polysaccharide and codonopsis clematidea Clarke's polysaccharide is measured and proved to the different reaction systems of employing such as Li Guirong etc. and Xiong Yuanjun respectively; The former also finds the scavenging action of Radix Codonopsis polysaccharide to hydroxyl radical free radical; The latter tests the generation that proof codonopsis clematidea Clarke polysaccharide can reduce MDA in vivo. and the anti-aging effects of Radix Codonopsis mainly shows in the following areas. and discovery rats such as Wang Shaoshen begin to give Radix Codonopsis to the geratic period from the postadolescence; Can delay the aging of hypothalamus one endocrine network through the degree of injury that alleviates the central nervous system; Improve the central nervous system function state indirectly; Thereby reaching the purpose of slow down aging. Wang Min etc. [71] find that plate party can improve senile organism IL one 2 levels, thereby repair the immunosenescence of aging body and play function in delaying senility. king's equality finds that plate party can improve the histologic lesion of subacute aging model mouse brain, thus slow down aging. Xu Aixia etc. [73] experiment proof Radix Codonopsis polysaccharide can the human body immunity improving function; Remove free radicals such as active oxygen in various degree; Reduce level of lipid peroxidation and raise NO in the body, improve the organism physiology function, the performance anti-aging effects.

1, Radix Codonopsis polysaccharide is to the abdominal cavity influence of lotus Sm ascites tumor cell mouse time-to-live.

1.1 S ₁₈₀The cultivation of going down to posterity of ascites tumor cell: aseptic extraction inoculation back 5d well-grown abdominal cavity lotus S ₁₈₀Mouse ascites, and (oncocyte content is about 510 to become tumor cell suspension with physiological saline solution by 1: 3 dilution proportion ⁷/ m), every mouse peritoneal is penetrated tumor cell suspension 0.2mL, goes down to posterity 1 time to keep S in per 5 days ₁₈₀The vigor of ascites tumor cell.Can find out by table 7, to abdominal cavity S ₁₈₀The increase in life span that the ascites tumor cell mouse is irritated stomach 1g/ (kgd) Radix Codonopsis polysaccharide is 34.29%, compares with negative control group, and P＜0.05 shows that polysaccharide is to abdominal cavity lotus S ₁₈₀The ascites tumor cell mouse has certain life prolongation effect, has certain antitumor action.

1.2 anti-tumor experiment: inoculation S ₁₈₀Behind the ascites tumor cell 24, be divided into experiment drug group 3 dosage, negative control group and positive controls (Cy) at random, 10 every group.The experiment drug group press 0.4mL/10g gastric infusion every day 1 time, 7d continuously, branch 0.5g/ (kgd), 1g/ (kgd) and three dosage of 2g/ (kgd); The gastric infusion next day of positive controls cyclophosphamide 50mg/kg is given 3 times; Negative control group is irritated the stomach normal saline every day, continuously 7d.Continue to observe animal to dead, write down the existence natural law of tumor-bearing mice, calculate the increase in life span of tumor-bearing mice

Table 7 various dose group medicine is to inoculation S ₁₈₀The influence of ascites tumor mice time-to-live

Compare * P＜0.05 with matched group

Resveratrol

Resveratrol chemistry by name 3; 4; 5-trihydroxy 1; 2 one stilbene, the non-flavonoid polyphenolic substance of genus stilbene class formation is one type and mainly has the polyphenol compound in the plants such as Fructus Vitis viniferae, Rhizoma et radix veratri (Radix Rhizoma Veratri), Rhizoma Polygoni Cuspidati. at present; People have at least found resveratrol and glycosides thereof in 72 kind of plant of 31 genus of 21 sections. in view of in its molecule three phenolic hydroxyl groups are arranged; People infer that it possibly be a kind of oxygen free radical scavenger. discover that resveratrol has very strong antioxidation, anti-radical action, also have effects such as immunomodulating, antitumor, cell death inducing simultaneously. and researcher mainly concentrates on to cancerous cell cardiovascular disease at present to the anti-oxidation function research of resveratrol; Nervous system and to aspects such as ischemic damage and reperfusion damages; This experiment is a model with mice increasing load swimming instruction 6W in the pond of flowing, and measures resveratrol to endurance training mouse cardiac muscle, quadriceps femoris, kidney, thymus, spleen, liver mitochondrion, erythrocytic SOD, GSH-Px activity and MDA content, and the content of liver mitochondrion free calcium; Inquire into the influence of resveratrol, for resveratrol provides experimental basis as the research and development of anti-fatigue nutrient tonic to big intensity endurance training anti-oxidative ability of mice.

1, resveratrol is to the influence of 6W endurance training mice portion of tissue organ SOD, GSH-PX activity and MDA content

Can find out from table 1; After the endurance training of 6w increasing load; B group mice quadriceps femoris, cardiac muscle, liver mitochondrion, spleen, thymus and erythrocyte sod activity all significantly are lower than A group (P＜0.05); C group mice quadriceps femoris, liver mitochondrion and spleen SOD activity are significantly higher than B group (P＜0.05). and table 2 is the result show; B group mouse cardiac muscle, spleen and erythrocytic GSH-Px activity all significantly are lower than A group (P＜0.05); C group mouse spleen and erythrocytic GSH-Px activity also significantly are lower than A group (P＜0.05), but the GSH-Px activity of quadriceps femoris and cardiac muscle is significantly higher than B group (P＜0.05). and table 3 is the result show, B group mice quadriceps femoris, liver mitochondrion, spleen and erythrocytic MDA content all are significantly higher than A group (P＜0.05); The MDA content of C group mice quadriceps femoris, liver mitochondrion all significantly is lower than B group (P＜0.05); Thymus and erythrocytic MDA content significantly are lower than the A group and B group (P＜0.05) result shows; B group mouse liver mitochondrion free calcium content is significantly higher than A group and C group (P＜0.05), does not have significant difference (P＞0.05) between A, C group.

Table 8 resveratrol is to the influence of 6W endurance training mice portion of tissue organ SOD, GSH-PX activity and MDA content

2, antioxidation, anti-radical action

Intravital oxidation level height takes place closely related with cancer; Especially crossing of reactive oxygen free radical expressed; Cause the proto-oncogene activation except causing body DNA oxidative damage and press down the inactivation of the basic ridge of pain; Normal expression and activity such as all right interference cell inner cell factor, metabolic enzyme, and these all have certain short cancer effect.Resveratrol has stronger antioxidant activity, removing free radical and influences the pharmacological action of arachidonic acid metabolic.Experiment in vitro is found, can suppress the synthetic of MC oxygen-derived free radicals from veratryl alcohol, and can suppress its propagation.Resveratrol has the obvious suppression effect to the erythrocyte hemolysis and the DNA damage of hydrogen peroxide-induced, and can suppress the generation of Hepar Leporis seu Oryctolagi homogenate malonaldehyde.Miura etc. prove that also the polyphenol hydroxy structure of resveratrol has antioxidant activity, can significantly suppress the generation of DNA in the oxidative damage process of hepatomicrosome, brain mitochondria and synaptosome.

3, suppress DNA and synthesize, block cell cycle and inducing tumor cell differentiation

The destruction of cell cycle regulating mechanism causes the growth of cell uncontrolled, and most of cancer-resisting substances can make cell cycle arrest at G1, S, a certain stage of M phase.Park etc. discover; Resveratrol is reduced cyclin cy-clinD1, cyclinD2 with the generation of dosage and time dependent mode inducing cell cyclin dependent kinase inhibition albumen p21; CyclinE and cell cycle protein dependent kinase 2 (cyclin dependent kinase 2; CDK2), CDK4, the expression of CDK6.And can reduce the CDK activity, cause the retardance of cell cycle.To the different types of tumors cell; The time phase of resveratrol retardance cell cycle is also different; Thereby its mechanism maybe for resveratrol through inducing the activation decomposing D NA repairase of cysteine proteinase easpase-3; Suppress the activity of RNA reductase through the cheese ammonia phthalidyl of removing the RNA reductase, thereby reduce the synthesis capability of DNA, reach the effect that suppresses cell proliferation.Cell cycle is the basic process of cell activities; The cell cycle process of different phase is under different regulatory factors promotes, to accomplish; The complex of forming like cyclin and cell cycle protein dependent kinase, PCNA (proliferating cell nu-clear antigen, PCNA), P53 etc.The effect that the resveratrol cell cycle is brought into play mainly is to reduce to be in G ₀To G ₁The cell of the cell of phase and retardance s phase, i.e. G ₁-S and S-G ₂Phase, thereby the propagation of inhibition tumor cell.Stervbo etc. are through breaking up the scale-model investigation of system to the human leukemia HL-60 cell; Find that resveratrol can cause the minimizing of granulocyte and macrophage; Show that resveratrol can induce the differentiation of human promyelocytic leukemia cell, thereby prove its inhibitory action tumor.

4, inducing cell is transferred and is died

4.1 (inhibitor of apoptosis pro-teins, IAPs) IAPs includes XIAP to IAP, clAP1, clAP2 and survivin.

It is one of former ridge of tumor cell escape cancer therapy drug lethal effect that the lAPs level raises, and can directly thrust 1 system caspase-3, the generation of the activity blocking-up apoptosis program of caspase-7.Survivin is one of important member of lAPs family; Foot is found the strongest survivin so far; High expressed in the most of tumors of the mankind is not but expressed at the sophisticated towel of organizing of normal development, and prompting survivin raised path between farm fields can be one of major reason that causes the pernicious differentiation of tumor cell.Aziz etc. draw report, to detect the expression that survivin is arranged in its skin behind the irradiation under ultraviolet ray nude mice, give resveratrol treatment after, find to have blocked the phosphorylation level of survivin from veratryl alcohol, advance i and reduce the protein expression of survivin.Also have.The scholar reports that resveratrol can reduce the expression of survivin in adult T cell leukemia's tumor cell, thus the apoptosis of inducing tumor cell.

4.2Bel-2 family

Bcl one 2 II of family have the albumen of a large amount of homologous amino acid sequences, comprise Bcl one 2 subtribes that suppress apoptosis and the Bax subtribe that promotes apoptosis, and the death that can promote cell is expressed in crossing of Bax, and Bcl-2 can suppress the function of Bax.Resveratrol can be reduced the Bcl-2 expression after acting on tumor cell, raises Bax and expresses, and Bel-2/Bax is descended, and the mitochondrion permeability of the membrane changes, activation caspase-9, Easpase-3 etc., the old `` of inducing apoptosis of tumour cell.The Bcl-xL gene belongs to the anti-apoptosis of Bcl one 2 gene families: gene, the important negativity regulating and controlling effect of performance in the path that apoptosis takes place.The Bcl-XL high expressed is relevant with tumor death and drug resistance.Johnson etc.Report; Resveratrol can suppress the transcription that MC relies on STAT3 and NF-KB; The be situated between expression of plain .1 conversion enzyme CKIs (cFLIP) and Bcl-xL of downward modulation cellular type Fas associated death region protein like cell; Final MAPK and the ATM.Chk2 one p53 signal path of activating starts apoptosis program.

4、3Fas

(APO-1 CD95) is the important member of death receptor family to Fas, can be called as the complex of inducing dead signal through combine to form one with Fas-L, activates easpase one 8 cell death inducings.Resveratrol can pass through CD95CD95L system induction apoptosis of tumor cells in some tumor cells, especially foot is in the tumor cell of number of C D95 and CD95L high expressed.After resveratrol acts on tumor cell, can detect increasing of CD95 and CD95LmRNA expression in asking in short-term,, induce its apoptosis through suppressing the propagation of tumor cell in the initial sum generating process that is expressed in tumor that raises CD95 and CD95L.But Delmas etc. discover; Resveratrol does not influence Fas and Fas-L expresses; But can cause that the Fas receptor redistributes on cell membrane; Change Fas associated death functional area and caspase 1 simultaneously and distribute, and then trigger the inhibition that apoptotic process and this effect do not receive Fas or Fas-L antagonist.Su etc. discover, but the expression of resveratrol inducing tumor cell Cdc42 activates ASKl and JNK, raises the expression of Fas-L, through the reaction induced apoptosis of tumor cells of signal cascade.

4.4p53

P53 has the mis repair gene as a kind of antioncogene under the normal condition, mutant cell is stagnated in G.Phase, the function of inducing cell differentiation and apoptosis, but abnormal change appears in about 50% human tumor.III1 such as She discover; Tumor cell is after resveratrol is handled, and the p53 molecule is regulated protein kinase and p38 kinases formation complex with the activated cells external signal, makes p53 at the 15th serine phosphorylation; Activate p53 one t and transfer p53 protein expression, inducing apoptosis of tumour cell.But the apoptosis that resveratrol causes only occurs in the cell of expressing wild type p53, and does not appear in the cell of p53 expression defective.

5,1 suppresses the cytochrome enzyme

The usefulness that jervic alcohol for antineoplastic medicine starts is relevant with its anti-xenobiotic carcinogenic activity in essence.Xenobiotic is meant the chemical substance in inanimate source, like insecticide, industrial pollutants and various synthetic compounds etc.With the smoking carcinogenesis is example, contains the aromatic hydrocarbon of a large amount and the part of dioxin receptor (AhR) in the flue gas, comprises benzopyrene (BaP) and anthracene (anthracenes) etc.Smoking is the cause of disease of 8 kinds of cancers at least, also is the arch-criminal of heart and lung diseases.Dioxin gains public acceptance to people's carcinogenesis at present.The carcinogenic main mechanism of flue gas is the dna mutation that the covalency adduct structure of peroxidating or the formation of DNA chain an ancient type of spoon causes.The adduct structure is that (for example B{iP} is through the Oxidation of cytochrome P 450 enzymes lA1, lA2, lBI (cYPlA1, lA2,181) by the various complex in the flue gas; Make these inertia flue gas complex molecule change highly mutagenic epoxydiol derivant into; Discharge free radical simultaneously, the latter can cause the oxidation of DNA to decrease part.BaP itself is the competitive part of dioxin receptor (Al rouses), podocyte pigment enzyme CYI, the rich inducer of wanting that lA transcribes.Discover that the CYP gene trans-activation that resveratrol can be through suppressing the AhR mediation and through suppressing the generation of free radical is controlled the tumor sudden change of starting.Rcvd etc. " research show that resveratrol in fact also is the competition thing of AhR, can a lot of organs in vitro and in vivo in, block the expression of CYPlA1 effectively.These results show that resveratrol is the inhibitor of Cytochrome P450.And in tumor cell, the cytochrome enzyme can press down cancer through other a kind of approach.Cytochrome P 450 enzymes cYPlB1 is overexpression in multiple human tumor cell, and can catalysis aromatic hydrocarbon hydroxylating.Discoveries such as Potter, resveratrol are converted into a kind of hydroxylation product under the effect of cytochrome P 450 enzymes CYPlRI, this product has been proved to be a kind of leukemia factor.This finds prompting, and the CYPlB1 in the tumor can spread into hydroxylation product through the catalysis resveratrol and suppress tumor growth, and CYPlB1 just plays a part the tumor suppression enzyme.

6, induce and separate toxenzyme

Separate toxenzyme and comprise glutathion-S transferring enzyme, uracil a pair of phosphoglucose aldehydic acid based transferase and 2 one menadione oxidoreductasees etc.Discover, can induce from veratryl alcohol and separate toxenzyme, become the chemical compound of non-activity to the xenobiotic conjugation of carcinogenecity, so that it is got rid of through metabolism.For example, quinone oxidoreductase (drug metabolism enzyme) activation in the resveratrol ability inducing culture Hepar Mus cancerous cell, and this enzyme is converted into a kind of quinone with carcinogen BaP and detoxifies.Therefore resveratrol is a kind of derivant of separating toxenzyme, is valuable chemopreventive agent.These separate transcribing of toxenzyme is inductive through antioxidant (electrophilic reaction).Though resveratrol can suppress the trans-activation of the CYP gene of xenobiotic mediation, to antioxidant (electrophilic reaction) inductive separate toxenzyme transcribe the unrestraint activity, so resveratrol can not suppress to separate the generation of toxenzyme.

7, suppress epoxy platform enzyme

Results of study such as Bhat refer to that cyclooxygenase (COX) can bring out cell carcinogenesis through 2 kinds of different approaches.A kind of approach is to make prostaglandin metabolism disorderly and bring out canceration.Organismic internal environment is stable to play an important role the homergy of prostaglandin to keeping, and its building-up process is controlled by free radical proceed.When the COX conversion of arachidonic acid forms PGG2 (prostate rope G class 2 subclass), need peroxy radical from arachidonic acid, to extract a hydrogen atom and inspire reaction.Because COX has the hydroperoxides enzymatic activity concurrently, further catalysis PCK; 2 are converted into PGH2 (PGH class 2 subclass), and this process discharges a highly active free radical intermediate product.This intermediate product has extremely strong oxidability, can attack a series of enzymes of prostaglandin route of synthesis back and make their inactivations, causes prostaglandin metabolism disorderly, thereby promotes cancer cell multiplication and suppress immune surveillance.Man's one approach is a COX activation carcinogen, destroys hereditary material, promotes canceration.Research shows, the cyclooxygenase-2 activity that resveratrol can be through suppressing COX is with the cyanogen peroxidase activity and (resveratrol suppresses the expression of COX through the activation of obstruction nuclear factor NF KB in tumor promotion stage inhibitory action.Therefore as the COX inhibitor, resveratrol is considered to the of great value therapeutic agent of multiple cancer.

8, CKIs kinases

Yu has studied the inhibitory action of 19 hydroxystilbenes (comprising resveratrol) to LCK (PTK), finds that the equal tool of nearly all hydroxyl late-maturing compounds suppresses active.PTK can activate the ATP terminal phosphate, makes the tyrosine residue phosphorylation of substrate protein white matter, and this process plays an important role in the information transmission that oncogene is expressed, and many malignant cells find that all certain specific PTK is activated or overexpression.Resveratrol is expected to play a significant role at anti-tumor aspect as ptk inhibitor.Proofs such as Atten, 6 kinds of hydroxy compounds such as resveratrol and 3 kinds of stilbene class oligomers all have inhibitory action in various degree to APC Protein kinase C (PKC).Enzyme kinetics experiment shows, the inhibitory action of PKC belonged to noncompetitive suppress.PKc is approved that in the important function of regulating cell information transfer connection physiologically actives such as the antitumor of its inhibitor, antiinflammatory also obtain paying attention to day by day.

9, antagonism estrogen and change autocrine growth regulatory factor

The experiment in vitro that Bhat etc. utilize Ishikawa endometrial carcinoma cell system to carry out finds that resveratrol can be brought into play good growth inhibited effect to endometrial carcinoma cell.In fact, Serrero etc. have proved that resveratrol is the antagonist of estrogen receptor (ER), are effective phytoestrogens." research shows that resveratrol (concentration is 10.5mol/L) can suppress the positive breast carcinoma MCF of the ER one 7 cell growth stimulation effects of estradiol (concentration is 10mol/L) mediation to greatest extent to Serrero etc., but does not influence the vigor of cell.See that from molecular level resveratrol is actually dose dependent ground antagonism MCF one 7 cell growth stimulation effects, this kind effect is by due to the estradiol reporter gene and progesterone receptor gene expression in the MCF-7 cell.Resveratrol also can suppress the propagation of estrogen receptor negative breast cancer cell MDA-MB 468.This discovery shows that except antagonism estrogen receptor mechanism, resveratrol also can be through changing the propagation that autocrine growth regulatory factor and its receptor expression in the MCF-7 cell suppress breast cancer cell.(concentration is 10 to resveratrol ⁵Mol/L) expression (these 3 kinds of factors play the effect that stimulates autocrine) of the somatomedin in inhibition transforming growth factor TGFa, pheochromocytoma (PC cell) source and insulin-like growth factor 1 receptor mrna.In addition, resveratrol improves the expression of long inhibitor TGF-β 2 mRNA of China, but does not influence the expression of TGF-β 1 sharp TGF-β 3.These phenomenons show that resveratrol suppresses breast cancer cell propagation through changing the autocrine growth regulatory factor.

10, inducing tumor cell differentiation

Under the normal cultured condition, people's promyelocyte HL-60 can infinite multiplication.Through scale-model investigation to HL one 60 cell differentiation systems; Find that resveratrol can cause that granulocyte and macrophage reduce; Suppress to mix people's H one thymus pyrimidine simultaneously; Show that resveratrol can induce the human promyelocytic leukemia cell to non-propagation phenotypic differentiation, thereby proof mortar veratryl alcohol also there is obvious inhibitory action in the tumor development stage.Think at present, Resveratrol Inducing swell and ache the mechanism of cell differentiation possibly be resveratrol can mobilize tumor cell effectively cell cycle from G ₁-G ₀Phase gets into the S phase.For untwisting in the s phase, duplicates cell DNA, if a large amount of cell accumulation is in the S phase. and the probability that dna replication dna, rearrangement and base pairing are made a mistake increases, and might cause that gene phenotype changes, and causes whole cell differentiation.

11, short apoptosis effect

Lin etc. discover that resveratrol has pro-apoptosis bioactivity to prostate tumor cells, and harmless to normal cell.Apoptosis is the initiative death process that cell self is regulated, its reaction that do not cause inflammation, and untoward reaction can so not take place in body, net and apoptosis is an oncotherapy research-individual key areas.Resveratrol has shown its potential using value in oncotherapy undoubtedly with the mode antitumor of inducing apoptosis of tumour cell.Narayanan is in Resveratrol Inducing thyroid carcinoma cell system and 2 strain folliculus shape thyroid carcinoma cells systems (FTC); Handle with the white hellebore of 1 μ mol/L～10 μ mol/L respectively; The showed cell external signal is regulated the activation and the nuclear translocation of kinases (MAPK) as a result, and the abundance of inscription propylhomoserin phosphorylation, c-fos, c-jun and the p21mRNA of p53 all has increase in addition.Detect nucleosome level and dna break through ELISA and test and show from veratryl alcohol all apoptosis-inducedly in 2 cell lines, these effects are suppressed by the transfection of the resistance oligonucleotide of sub-pifithrin-a of the inhibition of p53 or p53.Therefore, resveratrol is to promote the expression of p53 and the serine phosphorylation of p53 through Ras-MAPK and kinases one MAPK signal transduction pathway, to reach the cell death inducing effect.Nonsteroidal anti-inflammatory active gene one I (NAG-1) is one of member of transforming growth factor (TGF-β) superfamily.It is expressed and urgees cancer cell-apoptosis and be associated with resisting to swell and ache.The expression that resveratrol can be induced NAG-1 with concentration dependence mode is wanted early but resveratrol improves the expression ratio NAG-1 induction of tumor suppressor protein p53.This shows that the expression of the NAG 1 of Resveratrol Inducing possibly be to be mediated by the p53 expression.P53 connection site in the promoter region of NAG-I plays a crucial role in the p53 of control Resveratrol Inducing expresses.

Curcumin

Curcumin is a kind of plant polyphenol.Derive from rhizome such as Rhizoma Curcumae Longae, Radix Curcumae, Rhizoma Curcumae, Rhizoma Acori Graminei, chemical constitution is C21 H20 06.Molecular weight 368.37.The major production areas in the subtropical zone like China, India and Japan and other places.Over past ten years, both at home and abroad medical circle has been carried out system, extensive, deep research to it.Find that curcumin has effects such as antioxidation, infection, antiinflammatory, blood fat reducing, atherosclerosis and inhibition tumor growth. the state-run tumor of the U.S. is classified it as third generation cancer chemoprevention medicine.

1, regulation and control oncogene and antioncogene

Research shows that the inactivation of oncogene active and antioncogene is that tumorigenic reed is wanted mechanism.Kakar etc. have studied curcumin to the inductive CD-1 mouse skin of TPA c-ju; 1, c-b with " influence that myc proto-oncogene mRNA expresses, the TPA of 30nmM can make c-jun; the expression that p adds with c-myc proto-oncogene mRNA increases by 2～3 times; if but before using TPA 30min smear curcumin at the skin of back of mice, then can obviously suppress the expression of inductive these proto-oncogenes of TPA, the rejection ratio c-Bye that wherein c-jun and c-JOs is expressed is obvious more _.It is necessary that the high expressed of the inductive open gene of TPA is that tumor forms, and changes and ask factor c-jun/AP-1 in the signal transduction that the TPA induced tumor forms, to play an important role.Discover; 101; The curcumin of xmol/L just can suppress the activity that l cell is transcribed the sub-c-jun/AP-1 in ridge; Suppress c-jun/AP-1 and its combining of homologous amino acid sequence 5-TGAACTCAG-3, this possibly be the main cause that curcumin suppresses the gene expression that c-jun/AP 1 mediates.`` such as Chen one utilize RT.PCR and NorthernHolting technology to find that also curcumin can suppress the expression of smooth muscle cell c-myc and bcl_2mRNA, but the expression of antioncogene p53 mRNA is not then had influence.Researchs such as Han think that then curcumin can following survey B cell lymphoma BKS one 2 cell c-myc expression of gene and the expression of downward modulation p53 antioncogene, the old J of cytostatic effect.If in food, add 0.2% or 1% curcumin, promptly can suppress the inductive auspicious ten albinism mouse skin c-ras of TPA and the c-fos gene expression that start by DMBA.Breast cancer cell research shows not, and curcumin suppresses the expression of p53 mRNA, the expression of the antioncogene p21 mRNA that withers down, the expression of rise BAX mRNA.

3, downward modulation NF- _KB is active. and suppress iNOS, COX-2 is active

Cyclooxygenase (COX) is a kind of embrane-associated protein, is the biosynthetic rate-limiting enzyme of PGs, and this enzyme has COX-1 and two kinds of hypotypes of COX-2, discovers the confidential relation that has with tumor that increases of COX one 2 mRNA and protein expression.Zhang etc. carry out r curcumin inhibition bile acid and the Fo Bo ester is induced digestive tract SK-GT-SCC450; The research of IEC 1 and HCA-7 cell line COX-2 expression; The result finds that curcumin not only suppresses COX-2 mRNA and proteic expression, also suppresses the activity of COX-2 and returns.Discovery curcumins such as Goel both can suppress the growth of HT29 cell, and inhibitory action presentative time, dose-effect relationship again can obvious suppression COX-2 mRNA and proteic expression, but COXl is not then had influence.Induce punishment nitricoxide synthase (iNOS) in normal structure, seldom to express, but in a lot of tumor tissues, then present high expressed.The unusual focus of crypts (ACF) is considered to the earliest pathological changes of colon cancer.But the curcumin legendary small dragon with horns reaches through the activity that suppresses iNO% and COX-2 and reduces the effect that rat ACF forms.Lichuan Rhizoma Curcumae Longae rope such as Pan and metabolite thereof produce iNOS to activated table tennis phagocyte one RAw 264.7 of lipopolysaccharide (LPS) and study, and the result shows that curcumin obviously reduces the generation of the activated macrophage iNOS of LPS.NF-kB is a kind of important nuclear factor in the cell.Under the normal condition, NF-kB is present in the endochylema with the non-activity state, and it is the dimer that is made up of NF-kBl and Rel, combines the trimer that forms with its CKIs (IKB).Plmnmer etc.91 think that expression that curcumin suppresses colon cancer cell COX-2 is thereby that it suppresses the activity of NF-kB through the activity that suppresses IKK and finally is achieved.Sur1 etc. think that the preventive effect of curcumin is the activity that suppresses NF-KB through it, and the latter suppresses INOS again, and the expression of COX-2 realizes with activity.

4, to EGFR, the influence of PKC signal transduction pathway

Korutla etc. have studied curcumin to EGF-R ELISA (EGFR) tyrosine

Kinase whose activity and to the influence of the phosphorylation of the EGFR of EGF mediation, discover: it is sad that 1. the Rhizoma Curcumae Longae rope can suppress the kinase whose work of A431 cell EG-FR; 2. utilize the EGF of saturated concentration can promote to cultivate NIH3T3 cell EGFR tyrosine phosphorylation effect increase 4-9 doubly, this effect almost can be suppressed by curcumin fully; 3. curcumin can suppress the effect that EGF promotes the cell growth, but this effect is not to realize through the quantity that suppresses EGFR.Application curcumins such as Dorai are studied the EGFR signal transduction pathway of responsive (LNCaP) and insensitive (FC 1) the two kinds of prostate cancer cell lines of estrogen; Find that curcumin passes through the signal transduction that 3 kinds of different model of action influence EGFR, the proteic expression of downward modulation EG}R; The activity that suppresses Endogenous EGF R EGFR-TK; The activity that suppresses the inductive EGFR of part.Curcumin is the inhibition curcumin or the kinase whose inhibitor of multiple protein of EGFR signal transduction pathway, no matter is to protein kinase c (PKC), still egg is all had inhibitory action from kinases A (PKA), protamine kinase (CPK), phosphorylase kinase (PHK) etc.0.1 the curcumin of μ mol/L just can make the activity of PKC descend 15%.The Rhizoma Curcumae Longae rope of 15 μ mol/L or 20 μ mol/L can suppress the inductive PKC activity of TPA and reach 26%～60%, but the inhibitory action of curcumin to PKC proteic expression then do not have influence.It is active that experiment in vitro finds that also curcumin can suppress PKC through the antagonism Phosphatidylserine.

5, inducing cell cycle arrest and apoptosis apoptosis

In the tumor development process, mainly play the negative regulation effect, can check the tumor cell ramp.Give laboratory the mechanism that curcumin suppresses the growth of colorectal cancer LO.VO cell is studied, find: the cell after 1. curcumin is handled mainly concentrates on cell cycle S and G2/M phase, makes cell 4i can get into next cycle; 2. curcumin can cell death inducing, and the curcumin of 20 μ mol/L is made the trapezoid-shaped strips that apoptosis-specific can appear in DNA electrophoresis behind HJ cell 24～36h, and corresponding change also takes place in the morphology of cell, and raised path between farm fields is to see apoptotic body.Discovery curcumins such as Moragoda both can block the phase in G2/M with colorectal cancer HCT-166 cell through the activity that suppresses cell hilllock phase protein D and E. and fork is through stimulating caspase 1, the activity of casepase 1, the horizontal cell death inducing of reduction Bcl-xL.

6, suppressing the activity of urokinase and the secretion of MMP-9 grinds

Study carefully discovery; Curcumin also has the effect of anti metastasis, and urokinase is a kind of serine proteinase enzyme, by tumor cell or other emiocytosis; Quiet property uPA can through to the activation I of fibrinolysin " ii degraded extracellular matrix and basement membrane, thus promote invading bag and shifting of tumor cell.Fibronectin (FN) is the macromole glycoprotein in the extracellular matrix, combines with integrin receptor on the cell membrane, promotes the bull of cell to echo migration.Transforming growth factor 1 (TGF-β) can stimulate the release and the expression of migration, invasion and attack and the urokinase of mice horn cell; Curcumin not only can be blocked release that TGF-BI induces urokinase, suppress the synthetic of the beta induced FN of TGF-; Can also reduce TGF-β, the migration of stimulated cells and infiltration.Matrix metalloproteinase (MMPs) belongs to zinciferous metalloproteases family member.MMPO also claims gelatinase B or 92kB gelatinase, and its main degradation products is for constituting the Iv Collagen Type VI of the important framework ingredient of basement membrane.Lin etc. have inquired into migration, invasion and attack and the MMPO excretory influence of curcumin to high secondary liver cancer SK-Hep one 1 cells; Find MMP-9; Curcumin can with the mobility of SK-Hep one 1 cells and aggressivity suppress respectively 17.4% with the TGF-β of 70.6%:SK-Hep-I emiocytosis HCC and Huh_7 cell apparently higher than low metastatic capacity, but the SK-Hep-1 cell is urged MMP-9 and is divided secret effect to be suppressed by curcumin.Curcumin suppresses the activity of urokinase and the secretion of MMP-9 is the possible mechanism of its anti metastasis.

Selenocarrageenan

Selenium is one of nutrient of the definite needed by human of World Health Organization (WHO); Also be that at present various nutrients with immunoloregulation function (comprise that unique and opposing viral infection in the vitamin A, vitamin C/Zn/Mg) have the nutrient of direct relation; It is enhances human body immune system ability in several ways, effectively stops virus mutation, resists multiple disease of viral infection; And can effectively improve people's emotion, have resisting fatigue, anti-depressed effect.Selenium is the important component that constitutes glutathione peroxidase (GPX).Selenium Supplement can make the GPX increased activity, removes free radical and oxidation resistance thereby strengthen, protection body cell film, nucleic acid and proteinic normal configuration and function.

1, selenium is to immune influence

Research shows that selenium influences immunity of organism and mainly comprises 3 kinds of immunization wayses, i.e. cellular immunization, humoral immunization and nonspecific immunity.Selenium can promote the generation of interferon, and can increase the plain activity of γ-disturb external, enhances human body NK cytotoxicity, and do not damage target cell membrane.Selenium can also impel the secretion capacity of lymphocyte IL-1 and IL-2 significantly to strengthen, and the formation of immune stimulatory globulin improves the ability that body synthesizes antibody such as IgG, IgM.In addition, selenium for phagocyte to the chemotactic of virion, engulf and the process of killing all has influence in various degree.Discover, selenium can with the macrophage factor (MAF) the synergistic activation macrophage of living, thereby strengthen anti-tumor activity, can reduce macrophage again to lymphocytic inhibitory action simultaneously.

2, selenic antioxidation is to the inhibition of tumor

The existence of life depends on oxygen, and life entity is through oxidizing process acquisition activity energy needed.From this respect, oxygen is indispensable to life entity.But then, oxygen also possibly cause oxidative damage to life entity, and is described as Diplock: " keeping balance on the cutter ball of life between aspect oxygen metabolism optimum and pernicious." can cause the oxygen of damage to belong to active oxygen to body, free radical is wherein arranged, like ultra-oxygen anion free radical (O ₂ ^-) free radical (HO); Hydroperoxyl radical (HOO), organic peroxy free radical (ROO) etc.; These free radicals comprise that the endogenous free radical and the exogenous chemical factors that are produced by enzyme system in the body or non-enzyme system cause the free radical that produces; But the equal various biomacromolecules of attack are like nucleic acid, protein etc.It not only makes membrane molecule receive oxidative damage, and can change or destroy membrane structure and film function, causes body injury; Or produce biomolecule free radical (secondary free radical) with the living matter effect, and in its subsequent reactions, continue to cause damage, cause the cell biological function reduction, even degeneration causes cancerous protuberance.In the face of so serious free radical threatens; In the organism; Aerobe has formed a cover anti-oxidative defense system in evolution of long period of time, can on different links, block radical reaction, makes life process be able in the balance of the generation of reactive oxygen free radical and removing, normally carry out.In the body antioxidant system, selenium also is an important member.Prove at present; Selenium is the essential ingredient of the sweet skin peroxidase of paddy moon bright (GSH-Px); Contain 4 selenium among each molecule GSH-Px, the GSH-Px ability catalytic reduction type sweet skin of paddy moon bright (GSH) becomes the sweet skin of paddy moon bright (GSSG) of oxidized form, makes deleterious peroxide (ROOH) simultaneously; Be reduced into nontoxicly, and make H through based compound (ROH) ₂O ₂Decompose, the protection cell membrane does not receive the damage of peroxide.

3, selenium is to the influence of mouse tumor weight, tumor weight/body weight index

Visible by table 23, the 25d that continues medication, with the normal control ratio, the selenium of 3 kinds of dosage can both significantly reduce heavy, the tumor weight/body weight of the tumor of tumor-bearing mice, and tumor killing effect is remarkable.

Table 23 selenium is heavy to mouse tumor, the influence

of tumor weight/body weight index

Compare with matched group.* P＜0.05, * * P＜0.01; Compare Δ P＞0.05 with the interferon group

4, selenium is to the influence of macrophage phagocytic function, NK (NK) cytoactive

Can find out that by table 24 continue to selenium 15d and normal control ratio, the selenium of 3 all dosage can both significantly strengthen spleen natural killer cell activity and the peritoneal macrophage of mice and engulf the chicken red blood cell ability.

The nano red essence selenium of table 24 is heavy to mouse tumor, the influence

of tumor weight/body weight index

Microcrystalline Cellulose:

Consider that Caulis et Folium Brassicae capitatae, the Radix Astragali, Radix Salviae Miltiorrhizae, Radix Codonopsis, resveratrol, curcumin, selenocarrageenan contain sugar and vitamin is higher and the taste medicine does not meet the characteristics of modern's custom; And the operability of the course of processing we selected the food additive microcrystalline Cellulose for use, show in " one one kinds of multi-functional macromolecular materials of microcrystalline Cellulose " literary composition of on " Guangzhou chemical industry " 1993 the 21st the 3rd phases of volume, delivering according to the Zhu Yu of Guangzhou Inst of Chemistry, Chinese Academy of Sciences qin, Tang Liegui, Pan Songhan: microcrystalline Cellulose can be used as antiplastering aid.The microcrystalline Cellulose addition meets GB2760 " food additive use sanitary standard " for 15% of prescription, and microcrystalline Cellulose toxicity property ADI does not do particular provisions (FAO/WHO, 1994); Make its whole prescription have more reasonability and science like this.

The unique effect of various compositions more than comprehensive, the prescription ratio of this medicine is: Caulis et Folium Brassicae capitatae 6-15g, Radix Astragali 9-20g, Radix Salviae Miltiorrhizae 9-20g, Radix Codonopsis 6-12g, resveratrol 6-12g, curcumin 0.04-0.1g, selenocarrageenan 0.004-0.01g microcrystalline Cellulose 2-10g.

The effective ingredient of Caulis et Folium Brassicae capitatae is that sulfur is sweet; The effective ingredient of the Radix Astragali is a polysaccharide; The effective ingredient of Radix Salviae Miltiorrhizae extract is a saponin, and the effective ingredient of Radix Codonopsis extract is a saponin, and the effective ingredient of resveratrol extract is a polydatin; The effective ingredient of curcumin is that the effective ingredient of selenocarrageenan is a microcrystalline Cellulose.

The effective ingredient of medicine distributes as follows: 100g contains the sweet 375mg of effective composition Radix Raphani sulfur, saponin 11000mg, crude polysaccharides 10000mg, triterpene 2000mg.

Several kinds of raw materials are assisted mutually in whole prescription and are deposited mutually more than this specific embodiment; Bring out the best in each other; Make full side present effect preferably, meet the composite reason of Chinese medicine, product has tangible enhancing immunity, alleviating physical fatigue, anti-tumor function effect through these article of function test proof.

Claims

1. an antineoplastic health medicine, it is filled a prescription than being: Caulis et Folium Brassicae capitatae 6-15g, Radix Astragali 9-20g, Radix Salviae Miltiorrhizae 9-20g, Radix Codonopsis 6-12g, resveratrol 6-12g, curcumin 0.04-0.1g, selenocarrageenan 0.004-0.01g microcrystalline Cellulose 2-10g.

2. health medicine according to claim 1 is characterized in that, prescription is than being Caulis et Folium Brassicae capitatae 6g, Radix Astragali 9g, Radix Salviae Miltiorrhizae 9g, Radix Codonopsis 6g, resveratrol 6g, curcumin 0.04g, selenocarrageenan 0.004g microcrystalline Cellulose 2g.

3. health medicine according to claim 1 is characterized in that, prescription is than being Caulis et Folium Brassicae capitatae 15g, Radix Astragali 20g, Radix Salviae Miltiorrhizae 20g, Radix Codonopsis 6-12g, resveratrol 12g, curcumin 0.1g, selenocarrageenan 0.01g microcrystalline Cellulose 10g.

4. health medicine according to claim 1 is characterized in that, prescription is than doing,, Caulis et Folium Brassicae capitatae 11g, Radix Astragali 16g, Radix Salviae Miltiorrhizae 14g, Radix Codonopsis 9g, resveratrol 10g, curcumin 0.08g, selenocarrageenan 0.006g microcrystalline Cellulose 8g.

5. health medicine according to claim 1 is characterized in that, the effective ingredient of this medicine distributes as follows: 100g contains the sweet 375mg of effective composition Radix Raphani sulfur, saponin 11000mg, crude polysaccharides 10000mg, triterpene 2000mg.

6. a manufacture method of making said each the described health medicine of claim 1-5 is characterized in that, respectively the Radix Astragali, Radix Salviae Miltiorrhizae, Radix Codonopsis, resveratrol medicine is pulverized earlier; Cross the 60-80 mesh sieve, mix incorporation time control certain hour after getting its powder and other supplementary material weighing respectively; Carrying out capsule again fills; Filling process is controlled filling weight in strict accordance with inner quality standard, screens then, polishing, inner packing, outer package, at last according to touchstone check warehouse-in.

7. method according to claim 6, incorporation time control 45-50 minute.