CN102586220A - Method for producing candida utilis saccharomycetes enveloped dry powder bacteria - Google Patents
Method for producing candida utilis saccharomycetes enveloped dry powder bacteria Download PDFInfo
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- CN102586220A CN102586220A CN2012100559562A CN201210055956A CN102586220A CN 102586220 A CN102586220 A CN 102586220A CN 2012100559562 A CN2012100559562 A CN 2012100559562A CN 201210055956 A CN201210055956 A CN 201210055956A CN 102586220 A CN102586220 A CN 102586220A
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- candida utilis
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Abstract
The invention relates to a method for producing candida utilis saccharomycetes enveloped dry powder bacteria. Candida utilis saccharomycetes with high purity can be obtained, has very high activity, and is conveniently enveloped by envelope substances, so that the activity of the bacteria is prolonged. The method comprises the following steps of: (1) culturing a primary seed with 100 percent of purity of the candida utilis saccharomycetes through a standard method; (2) adopting an incubator, inoculating the primary purified seed bacteria to the incubator so as to be ventilated, stewed and cultured, and obtaining the candida utilis saccharomycetes with the purity of 98 percent, wherein the culturing temperature is 28 DEG C, the culturing time is 48 hours, and a culture medium adopts potato juice; (3) adopting a homogenizer to disperse the candida utilis saccharomycetes at low temperature and low speed, and separating mass at 4 DEG C; (4) enveloping at 4 DEG C-8 DEG C for 2-4 hours through the envelop substances; and (5) obtaining candida utilis saccharomycetes dry powder with high purity through low-temperature drying, and adding the candida utilis saccharomycetes dry powder into food and feed.
Description
One, technical field:
The present invention relates to a kind of bacterial classification method, especially relate to the working method that a kind of Candida utilis bacterium encapsulates the dry powder bacterium.
Two, background technology:
In the background technology, at present, in foodstuffs industry and fodder industry, use the main effect of Candida utilis bacterium to be: the Candida utilis bacteria growing is rapid, can a large amount of bacterium proteins be provided for the human and animal; This bacterium is in process of growth simultaneously, and plurality of enzymes preparations such as meeting extracellular proteinase, glycase, lypase help food digestion in animal body; This bacterium can also be synthesized vitamin B group in the humans and animals enteron aisle, like VitB
1, VitB
2, VitB
12Deng.Foodstuffs industry now and fodder industry all adopt liquid fermentation technology and semisolid fermentation technology to produce the Candida utilis bacterium.These two kinds shortcomings that technology exists: the pure bacterium of Candida utilis that can not turn out lot of pure; Can't encapsulate pure bacterium and make the biological activity of bacterium be difficult to prolong with encapsulating material; Simultaneously these two kinds of method production costs high, yield poorly, have high input, complex manufacturing and economic benefit be low, can not satisfy foodstuffs industry and fodder industry to encapsulating the quality and quantity demand of pure bacterium.
Liquid technology fermentative prodn Candida utilis bacterium: bacterium and meta-bolites are mingled in one, and make the growth of bacterium receive the inhibition of meta-bolites, can not reach the growth peak value.It is high to concentrate cost simultaneously, and is prone to discard the benefit materials in the meta-bolites, causes damage.Semisolid fermentation is produced the Candida utilis bacterium: bacterium and fermented product are grown together; Be difficult for obtaining purified yeast; Thereby can't encapsulate; Cause the vigor of bacterium producing the use reduction, the bacterium that produces with this method simultaneously just has the phenomenon of mass mortality in drying process, the viable bacteria amount requirement in causing can't guaranteeing to use.
Three, summary of the invention:
The present invention is in order to solve the weak point in the above-mentioned background technology; Provide a kind of Candida utilis bacterium to encapsulate the working method of dry powder bacterium, it can obtain highly purified Candida utilis bacterium, and the bacterium vigor is very high; Be convenient to encapsulate, thereby prolonged the vigor of bacterium with encapsulating material.
For realizing above-mentioned purpose, the technical scheme that the present invention adopts is:
A kind of Candida utilis bacterium encapsulates the working method of dry powder bacterium, it is characterized in that may further comprise the steps:
(1) standard law is cultivated the first order seed of Candida utilis bacterium 100% purity;
(2) adopt incubator, be inoculated into the enterprising gas that works of incubator to one-level purifying kind daughter bacteria and leave standstill cultivation, obtain about 98% the pure article of Candida utilis bacterium, culture temperature is 28 ℃, and incubation time is 48 hours, and substratum adopts the murphy juice substratum; The making method of murphy juice substratum is: the fresh potato of 200g is cut into small pieces, and the potato fritter adds water 1000ml, boils 30 minutes, and keeping the skin wet in the boiling part is 1000ml.Use murphy juice 200ml, water 800ml adds up to 1000ml, adds peptone 10g, glucose 20g, and sodium-chlor 5g, heating for dissolving is transferred pH6.5, sterilizes 15 minutes down for 115 ℃.
(3) adopt clarifixator low temperature low speed to carry out the Candida utilis bacterium and disperse, at 4 ℃ of homogeneous, clarifixator speed is 8000 rev/mins;
(4) encapsulate 2-4h with encapsulating material at 4 ℃-8 ℃;
(5) cryodrying obtains high purity Candida utilis bacterium dry powder, adds in food and the feed.
Encapsulating material is animal serum 3 ml of volume percent 3%, glycerine 10 ml of volume percent 3% and sucrose 5 grams of weight percent 5%.
Compared with prior art, the advantage and the effect that have of the present invention is following:
The present invention can well remedy the deficiency that exists in liquid fermenting and the semisolid fermentation; Can obtain the Candida utilis bacterium of 98% left and right sides purity, and the bacterium vigor is also very high, is convenient to encapsulate with encapsulating material; Thereby prolonged the vigor of bacterium, reduced bacterium death in use.This production technology process is simple simultaneously, cost is low, economic benefit is high, is well positioned to meet the user demand to the Candida utilis bacterium of foodstuffs industry and fodder industry.
Five, embodiment:
Embodiment:
(1) standard law is cultivated the first order seed of Candida utilis bacterium 100% purity;
(2) adopt incubator to be inoculated into the enterprising gas that works of incubator to one-level purifying kind daughter bacteria and leave standstill cultivation; The pure article culture temperature of Candida utilis bacterium of acquisition about 98% is 28 ℃, and incubation time is 48 hours, and substratum adopts the murphy juice substratum; The making method of murphy juice substratum is: the fresh potato of 200g is cut into small pieces; The potato fritter adds water 1000ml, boils 30 minutes, and keeping the skin wet in the boiling part is 1000ml.Use murphy juice 200ml, water 800ml adds up to 1000ml, adds peptone 10g, glucose 20g, and sodium-chlor 5g, heating for dissolving is transferred pH6.5, sterilizes 15 minutes down for 115 ℃.
(3) adopt clarifixator low temperature low speed to carry out the Candida utilis bacterium and disperse, 4 ℃ of branch matter, clarifixator speed is 8000 rev/mins.
(4) using volume percent is that 3% animal serum 3 ml, volume percent are that 3% glycerine 10 ml and weight percent are 5% sucrose 5 grams, encapsulates 2-4h at 4 ℃-8 ℃.
(5) cryodrying, temperature are subzero 8 ℃-subzero 10 ℃, and the time is 2-4 hours, obtain high purity Candida utilis bacterium dry powder, add in food and the feed.
Claims (2)
1. a Candida utilis bacterium encapsulates the working method of dry powder bacterium, it is characterized in that may further comprise the steps:
(1) standard law is cultivated the first order seed of Candida utilis bacterium 100% purity;
(2) adopt incubator, be inoculated into the enterprising gas that works of incubator to one-level purifying kind daughter bacteria and leave standstill cultivation, obtain about 98% the pure article of Candida utilis bacterium, culture temperature is 28 ℃, and incubation time is 48 hours, and substratum adopts the murphy juice substratum; The making method of murphy juice substratum is: the fresh potato of 200g is cut into small pieces, and the potato fritter adds water 1000ml, boils 30 minutes, and keeping the skin wet in the boiling part is 1000ml;
Use murphy juice 200ml, water 800ml adds up to 1000ml, adds peptone 10g, glucose 20g, and sodium-chlor 5g, heating for dissolving is transferred pH6.5, sterilizes 15 minutes down for 115 ℃;
(3) adopt clarifixator low temperature low speed to carry out the Candida utilis bacterium and disperse, at 4 ℃ of homogeneous, clarifixator speed is 8000 rev/mins;
(4) encapsulate 2-4h with encapsulating material at 4 ℃-8 ℃;
(5) cryodrying obtains high purity Candida utilis bacterium dry powder, adds in food and the feed.
2. a kind of Candida utilis bacterium according to claim 1 encapsulates the working method of dry powder bacterium, it is characterized in that: encapsulating material is the animal serum 3ml of volume percent 3%, glycerine 10 ml of volume percent 3% and sucrose 5 grams of weight percent 5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100559562A CN102586220A (en) | 2012-03-06 | 2012-03-06 | Method for producing candida utilis saccharomycetes enveloped dry powder bacteria |
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| CN2012100559562A CN102586220A (en) | 2012-03-06 | 2012-03-06 | Method for producing candida utilis saccharomycetes enveloped dry powder bacteria |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107373391A (en) * | 2017-07-24 | 2017-11-24 | 宁夏伊佳仁清真食品有限公司 | A kind of method that fermentation mutton is prepared using candida utili |
| CN116121085A (en) * | 2022-09-14 | 2023-05-16 | 大连理工大学 | Cold-resistant yeast suitable for low-temperature aquaculture and biocontrol application thereof |
-
2012
- 2012-03-06 CN CN2012100559562A patent/CN102586220A/en active Pending
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107373391A (en) * | 2017-07-24 | 2017-11-24 | 宁夏伊佳仁清真食品有限公司 | A kind of method that fermentation mutton is prepared using candida utili |
| CN116121085A (en) * | 2022-09-14 | 2023-05-16 | 大连理工大学 | Cold-resistant yeast suitable for low-temperature aquaculture and biocontrol application thereof |
| CN116121085B (en) * | 2022-09-14 | 2024-05-07 | 大连理工大学 | Cold-resistant yeast suitable for low-temperature aquaculture and biocontrol application thereof |
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Application publication date: 20120718 |