CN102586215A - 水稻抗除草剂蛋白及其在植物育种中的应用 - Google Patents
水稻抗除草剂蛋白及其在植物育种中的应用 Download PDFInfo
- Publication number
- CN102586215A CN102586215A CN2012100377899A CN201210037789A CN102586215A CN 102586215 A CN102586215 A CN 102586215A CN 2012100377899 A CN2012100377899 A CN 2012100377899A CN 201210037789 A CN201210037789 A CN 201210037789A CN 102586215 A CN102586215 A CN 102586215A
- Authority
- CN
- China
- Prior art keywords
- plant
- nucleic acid
- protein
- als
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000196324 Embryophyta Species 0.000 title claims abstract description 135
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 54
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 35
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 28
- 235000009566 rice Nutrition 0.000 title claims abstract description 28
- 230000002363 herbicidal effect Effects 0.000 title claims abstract description 22
- 239000004009 herbicide Substances 0.000 title claims abstract description 21
- 240000007594 Oryza sativa Species 0.000 title claims description 27
- 230000000740 bleeding effect Effects 0.000 title abstract 2
- 238000000034 method Methods 0.000 claims abstract description 45
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 35
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 35
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 35
- 230000014509 gene expression Effects 0.000 claims abstract description 14
- 235000018102 proteins Nutrition 0.000 claims description 32
- 230000008859 change Effects 0.000 claims description 23
- 230000009261 transgenic effect Effects 0.000 claims description 20
- CAAMSDWKXXPUJR-UHFFFAOYSA-N 3,5-dihydro-4H-imidazol-4-one Chemical compound O=C1CNC=N1 CAAMSDWKXXPUJR-UHFFFAOYSA-N 0.000 claims description 16
- 239000002773 nucleotide Substances 0.000 claims description 9
- 125000003729 nucleotide group Chemical group 0.000 claims description 9
- 102220534877 Importin subunit alpha-6_S627N_mutation Human genes 0.000 claims description 4
- 102200087432 rs104894685 Human genes 0.000 claims description 4
- 102220202916 rs775400768 Human genes 0.000 claims description 4
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 108010000700 Acetolactate synthase Proteins 0.000 abstract description 6
- 239000000969 carrier Substances 0.000 abstract description 3
- 241000209094 Oryza Species 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 description 37
- 230000008569 process Effects 0.000 description 15
- 102000004190 Enzymes Human genes 0.000 description 12
- 108090000790 Enzymes Proteins 0.000 description 12
- 150000001413 amino acids Chemical group 0.000 description 12
- 235000013311 vegetables Nutrition 0.000 description 12
- WTLNOANVTIKPEE-UHFFFAOYSA-N 2-acetyloxypropanoic acid Chemical compound OC(=O)C(C)OC(C)=O WTLNOANVTIKPEE-UHFFFAOYSA-N 0.000 description 7
- 240000008042 Zea mays Species 0.000 description 7
- 235000019504 cigarettes Nutrition 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 230000008029 eradication Effects 0.000 description 7
- PLUBXMRUUVWRLT-UHFFFAOYSA-N Ethyl methanesulfonate Chemical compound CCOS(C)(=O)=O PLUBXMRUUVWRLT-UHFFFAOYSA-N 0.000 description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 6
- 230000035772 mutation Effects 0.000 description 6
- 241000589158 Agrobacterium Species 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 235000005822 corn Nutrition 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 239000013598 vector Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 4
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 4
- 229940100389 Sulfonylurea Drugs 0.000 description 4
- 241000723873 Tobacco mosaic virus Species 0.000 description 4
- 241000700605 Viruses Species 0.000 description 4
- 238000003752 polymerase chain reaction Methods 0.000 description 4
- YROXIXLRRCOBKF-UHFFFAOYSA-N sulfonylurea Chemical class OC(=N)N=S(=O)=O YROXIXLRRCOBKF-UHFFFAOYSA-N 0.000 description 4
- 102000007469 Actins Human genes 0.000 description 3
- 108010085238 Actins Proteins 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- CUBCNYWQJHBXIY-UHFFFAOYSA-N benzoic acid;2-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=CC=C1.OC(=O)C1=CC=CC=C1O CUBCNYWQJHBXIY-UHFFFAOYSA-N 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 235000011089 carbon dioxide Nutrition 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 238000009396 hybridization Methods 0.000 description 3
- 230000008676 import Effects 0.000 description 3
- 210000003205 muscle Anatomy 0.000 description 3
- 238000003976 plant breeding Methods 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 150000003852 triazoles Chemical class 0.000 description 3
- CLQMBPJKHLGMQK-UHFFFAOYSA-N 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1H-imidazol-2-yl)nicotinic acid Chemical group N1C(=O)C(C(C)C)(C)N=C1C1=NC=CC=C1C(O)=O CLQMBPJKHLGMQK-UHFFFAOYSA-N 0.000 description 2
- 241000724328 Alfalfa mosaic virus Species 0.000 description 2
- 244000020551 Helianthus annuus Species 0.000 description 2
- 235000003222 Helianthus annuus Nutrition 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 101150017040 I gene Proteins 0.000 description 2
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 2
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical group OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 244000098338 Triticum aestivum Species 0.000 description 2
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
- 239000006035 Tryptophane Substances 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 238000006555 catalytic reaction Methods 0.000 description 2
- CVSVTCORWBXHQV-UHFFFAOYSA-N creatine Chemical compound NC(=[NH2+])N(C)CC([O-])=O CVSVTCORWBXHQV-UHFFFAOYSA-N 0.000 description 2
- 238000013016 damping Methods 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 229910017053 inorganic salt Inorganic materials 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 229940107700 pyruvic acid Drugs 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- AYEKOFBPNLCAJY-UHFFFAOYSA-O thiamine pyrophosphate Chemical compound CC1=C(CCOP(O)(=O)OP(O)(O)=O)SC=[N+]1CC1=CN=C(C)N=C1N AYEKOFBPNLCAJY-UHFFFAOYSA-O 0.000 description 2
- 229960004799 tryptophan Drugs 0.000 description 2
- FQVLRGLGWNWPSS-BXBUPLCLSA-N (4r,7s,10s,13s,16r)-16-acetamido-13-(1h-imidazol-5-ylmethyl)-10-methyl-6,9,12,15-tetraoxo-7-propan-2-yl-1,2-dithia-5,8,11,14-tetrazacycloheptadecane-4-carboxamide Chemical compound N1C(=O)[C@@H](NC(C)=O)CSSC[C@@H](C(N)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]1CC1=CN=CN1 FQVLRGLGWNWPSS-BXBUPLCLSA-N 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- QTJCKLQRJJTCIS-UHFFFAOYSA-N 2-hydroxybenzoic acid;pyrimidine Chemical class C1=CN=CN=C1.OC(=O)C1=CC=CC=C1O QTJCKLQRJJTCIS-UHFFFAOYSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 101150021974 Adh1 gene Proteins 0.000 description 1
- 241000589156 Agrobacterium rhizogenes Species 0.000 description 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
- 101710117679 Anthocyanidin 3-O-glucosyltransferase Proteins 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 241000209763 Avena sativa Species 0.000 description 1
- 235000007558 Avena sp Nutrition 0.000 description 1
- 235000000832 Ayote Nutrition 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 101000755953 Bacillus subtilis (strain 168) Ribosome maturation factor RimP Proteins 0.000 description 1
- 235000016068 Berberis vulgaris Nutrition 0.000 description 1
- 241000335053 Beta vulgaris Species 0.000 description 1
- 244000106835 Bindesalat Species 0.000 description 1
- 235000000318 Bindesalat Nutrition 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 240000007154 Coffea arabica Species 0.000 description 1
- 240000004270 Colocasia esculenta var. antiquorum Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 240000004244 Cucurbita moschata Species 0.000 description 1
- 235000009854 Cucurbita moschata Nutrition 0.000 description 1
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
- 238000012270 DNA recombination Methods 0.000 description 1
- 241000702421 Dependoparvovirus Species 0.000 description 1
- 235000002723 Dioscorea alata Nutrition 0.000 description 1
- 235000007056 Dioscorea composita Nutrition 0.000 description 1
- 235000009723 Dioscorea convolvulacea Nutrition 0.000 description 1
- 235000005362 Dioscorea floribunda Nutrition 0.000 description 1
- 235000004868 Dioscorea macrostachya Nutrition 0.000 description 1
- 235000005361 Dioscorea nummularia Nutrition 0.000 description 1
- 235000005360 Dioscorea spiculiflora Nutrition 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000219146 Gossypium Species 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 235000006350 Ipomoea batatas var. batatas Nutrition 0.000 description 1
- 235000010254 Jasminum officinale Nutrition 0.000 description 1
- 240000005385 Jasminum sambac Species 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 241000710118 Maize chlorotic mottle virus Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 241000209056 Secale Species 0.000 description 1
- 235000007238 Secale cereale Nutrition 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000046109 Sorghum vulgare var. nervosum Species 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- 241000700618 Vaccinia virus Species 0.000 description 1
- 241001464837 Viridiplantae Species 0.000 description 1
- 235000007244 Zea mays Nutrition 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 244000037672 biotech crops Species 0.000 description 1
- 150000005693 branched-chain amino acids Chemical class 0.000 description 1
- 229940027138 cambia Drugs 0.000 description 1
- 239000001390 capsicum minimum Substances 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 229960003624 creatine Drugs 0.000 description 1
- 239000006046 creatine Substances 0.000 description 1
- 238000006114 decarboxylation reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- KXZOIWWTXOCYKR-UHFFFAOYSA-M diclofenac potassium Chemical compound [K+].[O-]C(=O)CC1=CC=CC=C1NC1=C(Cl)C=CC=C1Cl KXZOIWWTXOCYKR-UHFFFAOYSA-M 0.000 description 1
- 235000004879 dioscorea Nutrition 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 244000037671 genetically modified crops Species 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- -1 naphthyl alcohol Chemical compound 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 235000021118 plant-derived protein Nutrition 0.000 description 1
- 210000002706 plastid Anatomy 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 230000009465 prokaryotic expression Effects 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 238000000734 protein sequencing Methods 0.000 description 1
- 235000015136 pumpkin Nutrition 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000001177 retroviral effect Effects 0.000 description 1
- 102220277134 rs776745497 Human genes 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000012090 tissue culture technique Methods 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- YWBFPKPWMSWWEA-UHFFFAOYSA-O triazolopyrimidine Chemical class BrC1=CC=CC(C=2N=C3N=CN[N+]3=C(NCC=3C=CN=CC=3)C=2)=C1 YWBFPKPWMSWWEA-UHFFFAOYSA-O 0.000 description 1
- SPOMEWBVWWDQBC-UHFFFAOYSA-K tripotassium;dihydrogen phosphate;hydrogen phosphate Chemical compound [K+].[K+].[K+].OP(O)([O-])=O.OP([O-])([O-])=O SPOMEWBVWWDQBC-UHFFFAOYSA-K 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 241000701447 unidentified baculovirus Species 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
- 238000009333 weeding Methods 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 201210037789 CN102586215B (zh) | 2011-09-06 | 2012-02-20 | 水稻抗除草剂蛋白及其在植物育种中的应用 |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201110261002 | 2011-09-06 | ||
CN201110261002.2 | 2011-09-06 | ||
CN 201210037789 CN102586215B (zh) | 2011-09-06 | 2012-02-20 | 水稻抗除草剂蛋白及其在植物育种中的应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102586215A true CN102586215A (zh) | 2012-07-18 |
CN102586215B CN102586215B (zh) | 2013-04-17 |
Family
ID=46475448
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 201210037789 Active CN102586215B (zh) | 2011-09-06 | 2012-02-20 | 水稻抗除草剂蛋白及其在植物育种中的应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN102586215B (zh) |
Cited By (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102986522A (zh) * | 2012-10-22 | 2013-03-27 | 北京金色农华种业科技有限公司 | 一种杂交水稻种子两优华占的生产方法 |
WO2014187312A1 (zh) * | 2013-05-23 | 2014-11-27 | 深圳市作物分子设计育种研究院 | 植物新型保持系及不育系建立及其用途 |
CN105104166A (zh) * | 2015-07-23 | 2015-12-02 | 上海市农业科学院 | 一种选育抗咪唑啉酮类除草剂粳稻的方法 |
CN105349623A (zh) * | 2014-08-13 | 2016-02-24 | 深圳市作物分子设计育种研究院 | 抗除草剂基因OsmALS的HRM检测方法和应用 |
CN105695493A (zh) * | 2016-04-12 | 2016-06-22 | 江苏省农业科学院 | 一种als突变型基因在抗除草剂方面的应用 |
CN105734071A (zh) * | 2016-04-22 | 2016-07-06 | 江苏省农业科学院 | Als突变型基因及其蛋白在抗除草剂方面的应用 |
CN105755024A (zh) * | 2016-04-12 | 2016-07-13 | 江苏省农业科学院 | Als突变型基因及其蛋白和应用 |
CN105779479A (zh) * | 2016-04-12 | 2016-07-20 | 江苏省农业科学院 | 一种als突变型基因及其在抗除草剂方面的应用 |
CN105821026A (zh) * | 2015-01-05 | 2016-08-03 | 上海市农业科学院 | 一种使植物具有抗咪唑啉酮类除草剂特性的乙酰乳酸合成酶及其应用 |
CN106636028A (zh) * | 2017-02-08 | 2017-05-10 | 上海市农业科学院 | 具有抗咪唑啉酮类除草剂活性的水稻蛋白质、其编码基因及应用 |
CN106636027A (zh) * | 2017-02-08 | 2017-05-10 | 上海市农业科学院 | 具有抗除草剂活性的小麦蛋白质、其编码基因及应用 |
CN106834260A (zh) * | 2017-02-08 | 2017-06-13 | 上海市农业科学院 | 具有抗咪唑啉酮类除草剂活性的玉米蛋白质、其编码基因及应用 |
CN106818347A (zh) * | 2017-02-23 | 2017-06-13 | 舒城县文禾种植专业合作社 | 一种高活性水稻育苗及后期富硒栽培的方法 |
CN106868028A (zh) * | 2017-03-02 | 2017-06-20 | 江苏省农业科学院 | 粳稻的als突变型基因及其蛋白和应用 |
CN106867977A (zh) * | 2017-01-20 | 2017-06-20 | 湖南杂交水稻研究中心 | 一种水稻抗除草剂蛋白与基因及其应用 |
CN107043761A (zh) * | 2017-02-08 | 2017-08-15 | 上海市农业科学院 | 具有抗除草剂活性的水稻蛋白质、其编码基因及应用 |
CN107090447A (zh) * | 2017-06-23 | 2017-08-25 | 江苏省农业科学院 | 使植物具有除草剂抗性的水稻als突变型蛋白、基因及其应用 |
CN107155873A (zh) * | 2017-07-17 | 2017-09-15 | 安徽省农业科学院水稻研究所 | 一种使杂交稻父本不结实的制种方法 |
CN107385053A (zh) * | 2017-08-08 | 2017-11-24 | 江苏省农业科学院 | 一种鉴定水稻产生als基因突变的四引物分子标记方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0730030A1 (en) * | 1986-08-26 | 1996-09-04 | E.I. Du Pont De Nemours And Company | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
CN101031646A (zh) * | 2004-07-30 | 2007-09-05 | 巴斯夫农业化学产品公司 | 抗除草剂的向日葵植物、编码抗除草剂的乙酰羟酸合酶大亚基蛋白的多核苷酸和使用方法 |
-
2012
- 2012-02-20 CN CN 201210037789 patent/CN102586215B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0730030A1 (en) * | 1986-08-26 | 1996-09-04 | E.I. Du Pont De Nemours And Company | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
CN101031646A (zh) * | 2004-07-30 | 2007-09-05 | 巴斯夫农业化学产品公司 | 抗除草剂的向日葵植物、编码抗除草剂的乙酰羟酸合酶大亚基蛋白的多核苷酸和使用方法 |
Non-Patent Citations (1)
Title |
---|
宋贵生等: "水稻乙酰乳酸合成酶基因的克隆和功能分析", 《中国农业科技导报》 * |
Cited By (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102986522A (zh) * | 2012-10-22 | 2013-03-27 | 北京金色农华种业科技有限公司 | 一种杂交水稻种子两优华占的生产方法 |
WO2014187312A1 (zh) * | 2013-05-23 | 2014-11-27 | 深圳市作物分子设计育种研究院 | 植物新型保持系及不育系建立及其用途 |
CN104837334A (zh) * | 2013-05-23 | 2015-08-12 | 深圳市作物分子设计育种研究院 | 植物新型保持系及不育系建立及其用途 |
CN105349623A (zh) * | 2014-08-13 | 2016-02-24 | 深圳市作物分子设计育种研究院 | 抗除草剂基因OsmALS的HRM检测方法和应用 |
CN105821026A (zh) * | 2015-01-05 | 2016-08-03 | 上海市农业科学院 | 一种使植物具有抗咪唑啉酮类除草剂特性的乙酰乳酸合成酶及其应用 |
CN105104166A (zh) * | 2015-07-23 | 2015-12-02 | 上海市农业科学院 | 一种选育抗咪唑啉酮类除草剂粳稻的方法 |
CN105104166B (zh) * | 2015-07-23 | 2021-05-07 | 上海市农业科学院 | 一种选育抗咪唑啉酮类除草剂粳稻的方法 |
CN105779479B (zh) * | 2016-04-12 | 2017-08-25 | 江苏省农业科学院 | 一种als突变型基因及其在抗除草剂方面的应用 |
CN105695493A (zh) * | 2016-04-12 | 2016-06-22 | 江苏省农业科学院 | 一种als突变型基因在抗除草剂方面的应用 |
CN105755024A (zh) * | 2016-04-12 | 2016-07-13 | 江苏省农业科学院 | Als突变型基因及其蛋白和应用 |
CN105779479A (zh) * | 2016-04-12 | 2016-07-20 | 江苏省农业科学院 | 一种als突变型基因及其在抗除草剂方面的应用 |
WO2017177765A1 (zh) * | 2016-04-12 | 2017-10-19 | 江苏省农业科学院 | 一种als突变型基因在抗除草剂方面的应用 |
CN105734071A (zh) * | 2016-04-22 | 2016-07-06 | 江苏省农业科学院 | Als突变型基因及其蛋白在抗除草剂方面的应用 |
CN106867977A (zh) * | 2017-01-20 | 2017-06-20 | 湖南杂交水稻研究中心 | 一种水稻抗除草剂蛋白与基因及其应用 |
CN106867977B (zh) * | 2017-01-20 | 2018-06-05 | 湖南杂交水稻研究中心 | 一种水稻抗除草剂蛋白与基因及其应用 |
CN107043761A (zh) * | 2017-02-08 | 2017-08-15 | 上海市农业科学院 | 具有抗除草剂活性的水稻蛋白质、其编码基因及应用 |
CN106834260A (zh) * | 2017-02-08 | 2017-06-13 | 上海市农业科学院 | 具有抗咪唑啉酮类除草剂活性的玉米蛋白质、其编码基因及应用 |
CN106636027A (zh) * | 2017-02-08 | 2017-05-10 | 上海市农业科学院 | 具有抗除草剂活性的小麦蛋白质、其编码基因及应用 |
CN106636028A (zh) * | 2017-02-08 | 2017-05-10 | 上海市农业科学院 | 具有抗咪唑啉酮类除草剂活性的水稻蛋白质、其编码基因及应用 |
CN106818347A (zh) * | 2017-02-23 | 2017-06-13 | 舒城县文禾种植专业合作社 | 一种高活性水稻育苗及后期富硒栽培的方法 |
CN106868028A (zh) * | 2017-03-02 | 2017-06-20 | 江苏省农业科学院 | 粳稻的als突变型基因及其蛋白和应用 |
CN106868028B (zh) * | 2017-03-02 | 2019-12-06 | 江苏省农业科学院 | 粳稻的als突变型基因及其蛋白和应用 |
CN107090447A (zh) * | 2017-06-23 | 2017-08-25 | 江苏省农业科学院 | 使植物具有除草剂抗性的水稻als突变型蛋白、基因及其应用 |
CN107155873A (zh) * | 2017-07-17 | 2017-09-15 | 安徽省农业科学院水稻研究所 | 一种使杂交稻父本不结实的制种方法 |
CN107385053A (zh) * | 2017-08-08 | 2017-11-24 | 江苏省农业科学院 | 一种鉴定水稻产生als基因突变的四引物分子标记方法 |
Also Published As
Publication number | Publication date |
---|---|
CN102586215B (zh) | 2013-04-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102586215B (zh) | 水稻抗除草剂蛋白及其在植物育种中的应用 | |
CN102559646B (zh) | 小麦抗除草剂蛋白及其在植物育种中的应用 | |
CN103215243B (zh) | 甘蓝型油菜抗除草剂蛋白及其在植物育种中的应用 | |
CN105755024B (zh) | Als突变型基因及其蛋白和应用 | |
CN103103176B (zh) | 玉米抗除草剂蛋白及其在植物育种中的应用 | |
CN105779479B (zh) | 一种als突变型基因及其在抗除草剂方面的应用 | |
CN105624177A (zh) | 一种抗虫融合基因、编码蛋白、载体及其应用 | |
CN107217044A (zh) | 使植物具有除草剂抗性的水稻als突变型蛋白及其应用 | |
WO2017177765A1 (zh) | 一种als突变型基因在抗除草剂方面的应用 | |
Yellisetty et al. | In planta transformation of sorghum (Sorghum bicolor (L.) Moench) using TPS1 gene for enhancing tolerance to abiotic stresses | |
CN105734071A (zh) | Als突变型基因及其蛋白在抗除草剂方面的应用 | |
US20140380525A1 (en) | Plants having enhanced nitrogen efficiency | |
CN106755019B (zh) | 一种小麦als突变型基因及其蛋白在抗除草剂方面的应用 | |
CN101608184B (zh) | 棉花促丝裂原活化蛋白激酶基因GhMAPK16的克隆及其应用 | |
CN101516180A (zh) | 早熟的转基因植物 | |
CN104293802A (zh) | 百脉根erf类转录因子、其编码基因及表达载体和应用 | |
CN111826364B (zh) | 一种抗病虫害相关基因及其应用 | |
CN103773795A (zh) | 具有增强的产量相关性状的植物及其生产方法 | |
CN106868027B (zh) | 粳稻als突变型基因及其蛋白在抗除草剂方面的应用 | |
CN102304526B (zh) | 小金海棠铁调控转运体基因在提高植物铁含量中的用途 | |
Okeyo-Ikawa et al. | In planta seed transformation of Kenyan cowpeas (Vigna unguiculata) with P5CS gene via Agrobacterium tumefaciens. | |
CN108034662A (zh) | 小麦条锈菌pstg_06025基因在条锈病防治中的应用和抗条锈菌小麦的培育方法 | |
CN114736280A (zh) | ZmROA1蛋白在调控植物耐密性中的应用 | |
CN104334731A (zh) | 具有一种或多种增强的产量相关性状的植物和其生产方法 | |
CN103667340A (zh) | 一种可稳定遗传的抗虫转基因玉米的构建方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: XINGWANG INVESTMENT PTY LTD. Effective date: 20140115 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20140115 Address after: Futian District Che Kung Temple Tairan Industrial Zone in Shenzhen city of Guangdong province 518040 213 industrial buildings 3B--2 Patentee after: Shenzhen Xingwang Biological Seed Industry Co., Ltd. Patentee after: Xingwang Investment Pty Ltd. Address before: Futian District Che Kung Temple Tairan Industrial Zone in Shenzhen city of Guangdong province 518040 213 industrial buildings 3B--2 Patentee before: Shenzhen Xingwang Biological Seed Industry Co., Ltd. |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20170623 Address after: 518107 Guangdong province Shenzhen Guangming New District Feng Xin Lu Xinjian Xing Technology Industrial Park A6 Building 2 floor West Patentee after: Shenzhen Xingwang Biological Seed Industry Co., Ltd. Address before: Futian District Che Kung Temple Tairan Industrial Zone in Shenzhen city of Guangdong province 518040 213 industrial buildings 3B--2 Co-patentee before: Xingwang Investment Pty Ltd. Patentee before: Shenzhen Xingwang Biological Seed Industry Co., Ltd. |
|
CP02 | Change in the address of a patent holder | ||
CP02 | Change in the address of a patent holder |
Address after: 518100 2C unit, Guangming science and Technology Park, China Merchants attraction 3009, Guangming New District, Shenzhen, Guangdong, B6 Patentee after: Shenzhen Xingwang Biological Seed Industry Co., Ltd. Address before: 518107 west of 2 A6, New Building Technology Industrial Park, Feng Xin Road, Guangming New District, Shenzhen, Guangdong. Patentee before: Shenzhen Xingwang Biological Seed Industry Co., Ltd. |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20210218 Address after: 518035 unit 2c, building B6, Guangming Science Park, China Merchants Group, 3009 sightseeing Road, Guangming New District, Shenzhen City, Guangdong Province Patentee after: Shenzhen Xingwang Biological Seed Industry Co.,Ltd. Patentee after: SHENZHEN JIETIAN MODEL BIOTECHNOLOGY Co.,Ltd. Address before: 518100 2C unit, Guangming science and Technology Park, China Merchants attraction 3009, Guangming New District, Shenzhen, Guangdong, B6 Patentee before: Shenzhen Xingwang Biological Seed Industry Co.,Ltd. |