CN102580998B - Method for preparing solid repair agent by using protein peptide and repairing soil by using soil repair agent - Google Patents
Method for preparing solid repair agent by using protein peptide and repairing soil by using soil repair agent Download PDFInfo
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- CN102580998B CN102580998B CN201210040772.9A CN201210040772A CN102580998B CN 102580998 B CN102580998 B CN 102580998B CN 201210040772 A CN201210040772 A CN 201210040772A CN 102580998 B CN102580998 B CN 102580998B
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Abstract
The invention provides a method for preparing a solid repair agent by using protein peptide and repairing soil by using the soil repair agent. The method comprises the steps of: preparing the protein peptide prepared by using a method in the patent ZL200610019063.7 or the protein peptide prepared by using protein-containing waste according to the following formula in site, wherein the formula comprises 1) 5-10 percent of protein peptide, 2-6 percent of molasses and the balance of water; 2) 5-10 percent of protein peptide, 0.5-1.0 percent of laccase crude product, 1-2 percent of laccase strain and the balance of water; and 3), 5-10 percent of protein peptide and the balance of water. The formula 1 is suitable for the soil in which plants are planted and which is seriously polluted by heavy metal ions; the formula 2 is suitable for the soil in which plants are planted and which is seriously polluted by heavy metal ions and is polluted by polycyclic aromatic hydrocarbon; and the formula 3 is suitable for directly acting on conventional polluted soil with light pollution. The protein peptide soil repair agent can be used as a nutrient in which a microorganism or indigenous microorganism for promoting the enrichment of the plants to the heavy metal ions, so that the polluted soil is repaired. The soil repair agent has low price, high efficiency and good effect.
Description
Technical field
What the present invention relates to is contaminated soil remediation improving technology, protein peptides is particularly utilized to prepare the method for soil-repairing agent and rehabilitating soil thereof, utilize this soil-repairing agent can promote the quick growth of additional microorganism in soil, indigenous microorganism, also can promote the quick growth of plant simultaneously, strengthen the enrichment of microorganism, plant heavy metal ion, realize the pollution sources periphery agricultural land soil reparations such as city, mining site, chemical plant, and realize with the double environmental protection object of useless pollution treatment.
Background technology
Soil is one of elemental of environment, has irreplaceable effect in natural ecosystems.Along with the development that industrialization, urbanization and agricultural are highly intensive, three industrial wastes, the pollution of agrochemical, agricultural chemicals is more and more serious, due to the pollution that heavy metal chemicals etc. brings, cause increasing agricultural land soil Quality Down, agricultural product and ecological environment are brought and has a strong impact on.Soil pollution destroys the 26S Proteasome Structure and Function of farmland ecosystem.Soil pollution not only changes soil physico-chemical property, soil is degenerated, and polluter hinders or suppresses fauna composition and the vital movement of edaphon, affect conversion and the energy exchange of soil nutrient, crop failure, seed deterioration, agricultural quality is caused to decline, soil reduced or loses production capacity, causing the destruction of soil resource, the agroecological environment circulatory system worsened or interrupts.
The treatment measures of soil pollution generally have: engineering measure reparation, physical chemistry reparation, chemical redemption and biological prosthetic.Engineering measure, physical chemistry reparation and chemical redemption all various degrees shortcoming or cause secondary pollution.Biological prosthetic is a kind of new method utilizing biotechnology pollution administration soil.Utilize the heavy metal in biological reduction, purification soil or reduce heavy metal toxicity.Because the method is effective, be easy to operation, be day by day subject to people's attention, become the focus of contaminated soil remediation research.
Current bioremediation technology mainly utilizes the plant Adsorption of Heavy Metals with high summation.Microorganism is the important composition person of soil ecosystem, and it not only can indicate the ecosystem stability of contaminated soil, and has huge potential biological prosthetic function.Therefore, utilize plant and microorganism synergy regulating plant growth, administer heavy metal pollution and polycyclic aromatic hydrocarbons contaminated there is considerable meaning.
At present, biotechnology repairing polluted soil is utilized mainly to carry out from three aspects, the first, select hyperaccumulative plant; The second, strengthen the interaction of microorganism and plant, namely by effects such as microorganism enrichment heavy metal ion, attenuating toxicity, change valence states, promote absorption or the enrichment of plant heavy metal ion to reach the object of rehabilitating soil.Three, soil-repairing agent is applied, the soil-repairing agent range of goods reported at present is more, but mainly repair lean soil, its main component is containing higher nitrogen, phosphorus, potassium and biological nitrogen fixation bacterium etc., about repairing polluted soil, especially the soil of heavy-metal pollution, not yet there is concrete renovation agent commodity selling, part research report was tested compositions such as applying carbohydrate, nitrate, phosphate, sylvite or was directly applied the reparation of organic fertilizer for contaminated soil, show that there is certain repairing effect, but not yet industrialization and do not have concrete commodity selling.Report is had not yet to see as soil-repairing agent about protein peptides, current protein peptides is mainly used in protein foaming agent (food or fire foam) or protein foam concrete blowing agent, its preparation technology is usually with NaOH or hydrochloric acid hydrolysis, part is quick lime hydrolysis, due to hydrochloric acid, protein peptides prepared by sodium hydroxide hydrolysis has chlorion and sodium ion, to microorganism, the growth of plant can produce salt and poison, thus soil-repairing agent can not be used as, but quick lime the protein peptides that is hydrolyzed out and can directly using according to protein peptides prepared by ZL200610019063.7 patented technology, because calcium ion can by organic acid, carbon dioxide in air is neutralized into calcium salt, no matter be to plant, microorganism, soil is all harmless, this protein peptides can directly be prepared as soil-repairing agent or through various combination, for the soil remediation of different pollution level.
Summary of the invention
The object of the invention is to propose a kind of utilize protein peptides production cost low, effective soil remediation agent prescription and the method for soil remediation.This protein peptides utilizes patented technology (ZL 200610019063.7) to produce, or with quick lime hydrolysis containing albumen discarded object produce.Realize containing the recycling of albumen discarded object and the quick reparation to contaminated soil, and realize with the double environmental protection object of useless pollution treatment.
The present invention is achieved in that
1, protein peptides soil-repairing agent is pressed this proportions: protein peptides 5-10%, molasses 2-6%, all the other are water.Extemporaneous preparation also stirs, then is sprayed on and kind is implanted with plant and the heavier soil of polluted heavy metals ion, can realize contaminated soil reparation.
Molasses are sugar refinery discarded objects, sugary about 50%, and simultaneously containing trace element, biotin, the materials such as amino acid.
2, by protein peptides according to this proportions: protein peptides 5-10%, laccase semifinished product (a kind of enzyme preparation of decomposing polycyclic aromatic hydrocarbon, sterling potency unit about 12000) 0.5%-1.0%, laccase bacterium 1%-2%(ZL200710051995.4), all the other are water.Extemporaneous preparation also stirs, then be sprayed on kind be implanted with plant and polluted heavy metals ion heavier while again by polycyclic aromatic hydrocarbons contaminated soil, can realize contaminated soil reparation.
3, directly protein peptides solution is used: by protein peptides 5-10%, all the other are water, extemporaneous preparation, then are sprayed on the lighter soil of conventional pollution, can realize contaminated soil remediation.
Why want extemporaneous preparation, reason is that this protein peptides can not add anticorrisive agent, otherwise microorganism can not grow, and also have impact to a certain degree to plant growth and environment, protein peptides stoste itself is in alkalescence, can preserve 1-2 without the need to adding anticorrisive agent.
Protein peptides soil-repairing agent of the present invention is sprayed onto soil surface, only need about 5 days soil surfaces can grow one deck white micropopulation, mainly fungi, actinomycetes (bacterium is in soil surface and deep layer, do not form mycelium and spore, be difficult on the surface find out), also can promote the quick growth of plant, in the soil sprayed simultaneously, plant growth is fast, the florescence is long, plant is tall and big, and this feature is just being suitable for the reparation of contaminated soil.
This protein peptides soil-repairing agent can be used as the nutritional agents of additional microorganism or indigenous microorganism, promote that microorganism is to the enrichment of soil heavy metal ion, alleviate the pollution of heavy metal ion to around water source, change the valence state of heavy metal ion simultaneously, reduce the toxicity of heavy metal ion to plant, accelerate quick growth of plant, also accelerate, strengthen the enrichment of plant heavy metal ion, by utilization or the transfer of aboveground vegetation part, realize the reparation of contaminated soil.Realize the pollution sources periphery agricultural land soil reparations such as city, mining site, chemical plant, and realize with the double environmental protection object of useless pollution treatment.
The present invention has biochemical basis, in accelerate growth of microorganism, protein peptides contains fast more than 3 times of albumen fertilizer than common, because fertilizer need produce acid protease by microorganism, alkali protease, it is protein peptides that the various protease such as trypsase decomposes protein substance in fertilizer, can utilize through being converted into amino acid again, its process belongs to power consumption process, and the time is quite long, and the protein peptides soil-repairing agent that the present invention proposes spans this process, microorganism can Direct Resolution protein peptides (itself containing a large amount of amino acid), be converted into amino acid again to utilize, simultaneously, namely the quick growth of microorganism and plant shows that both interaction relationships are strengthened, also the enrichment of plant heavy metal ion is just strengthened, also the quick reparation of contaminated soil is just achieved, as additional laccase bacterium and laccase also achieve the in-situ immobilization of polycyclic aromatic hydrocarbon.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further described.
Embodiment 1
For the soil of heavy contamination, by protein peptides by following proportions: protein peptides 5-10%, molasses 2-6%, all the other are water.Extemporaneous preparation also stirs, be sprayed on the soil that the heavier kind of polluted heavy metals ion is implanted with plant again, add the amount of molasses, can implement according to the heavy metal ion content ratio of your soil, if higher, then need to add molasses 5-6%, the object of adding molasses wishes that additional microorganism and indigenous microorganism produce the materials such as a certain amount of acids, alcohol, polysaccharide through metabolism, can a certain amount of heavy metal ion of chemisorbed, alleviate the toxicity of heavy metal ion, promote the fast enriching of plant heavy metal ion.Spray method, after the protein peptides renovation agent prepared is sprayed onto soil, waters in a large number, until pollute bottom, but can not penetrate renovation agent solution.
Embodiment 2
Again by polycyclic aromatic hydrocarbons contaminated soil while heavier for heavy-metal pollution, by protein peptides by following proportions: protein peptides 5-10%, laccase semifinished product (a kind of enzyme preparation of decomposing polycyclic aromatic hydrocarbon, sterling potency unit about 12000) 0.5%-1.0%, laccase bacterium 1%-2%, all the other are water.Extemporaneous preparation also stirs, then is sprayed in above-mentioned soil, waters in a large number, until pollute bottom.
Each component of soil-repairing agent of the present invention can adopt commercially available prod, or self-control.
Claims (4)
1. utilize the soil-repairing agent that quick lime hydrolysis is prepared containing the protein peptides that albumen discarded object is produced, it is characterized in that formula is: protein peptides 5-10%, molasses 2-6%, all the other are water.
2. utilize the soil-repairing agent that quick lime hydrolysis is prepared containing the protein peptides that albumen discarded object is produced, it is characterized in that formula is: protein peptides 5-10%, laccase semifinished product 0.5%-1.0%, laccase bacterium 1%-2%, all the other are water.
3. the method for the soil-repairing agent repairing polluted soil utilizing quick lime hydrolysis to prepare containing the protein peptides that albumen discarded object is produced, it is characterized in that the formula of soil-repairing agent according to claim 1, prepare at the scene and stir, be sprayed on again and kind be implanted with plant and the heavier soil of polluted heavy metals ion, realize the reparation of contaminated soil.
4. the method for the soil-repairing agent repairing polluted soil utilizing quick lime hydrolysis to prepare containing the protein peptides that albumen discarded object is produced, it is characterized in that the formula of soil-repairing agent according to claim 2, prepare at the scene and stir, be sprayed on again and kind be implanted with plant and polluted heavy metals ion is heavier again by polycyclic aromatic hydrocarbons contaminated soil, realize the reparation of contaminated soil.
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| CN105294193A (en) * | 2015-10-08 | 2016-02-03 | 湖北大学 | Preparation method for nitrogen-loss-free and full water-soluble calcium polypeptide and application thereof in golf course |
| CN105272563A (en) * | 2015-10-12 | 2016-01-27 | 湖北大学 | Hydrated polypeptide compound for prolonging plant flowering period, preparation method and use method thereof |
| CN105290102A (en) * | 2015-10-26 | 2016-02-03 | 石阳 | Soil heavy metal treatment device and soil heavy metal treatment method |
| CN105315041A (en) * | 2015-11-16 | 2016-02-10 | 湖北大学 | Preparation method and application of calcium polypeptide promoter for improving soil micro-ecological population quantity |
| CN105461386A (en) * | 2015-11-16 | 2016-04-06 | 湖北大学 | Water-soluble carboxyl-calcium coupled polypeptide and preparation method and application thereof |
| CN105503332A (en) * | 2015-12-11 | 2016-04-20 | 湖北大学 | Bi-carboxyl coupling calcium polypeptide as well as preparation method thereof and application thereof in aspect of prolonging strawberry fruit production period |
| CN106085446A (en) * | 2016-06-17 | 2016-11-09 | 战锡林 | Polycyclic aromatic hydrocarbon pollution repair materials |
| CN106187442A (en) * | 2016-07-05 | 2016-12-07 | 袁广来 | Liquid peptidase biological organic fertilizer |
| CN106278581A (en) * | 2016-08-08 | 2017-01-04 | 袁广来 | Liquid peptidase microbe soil restoration agent |
| CN106966829A (en) * | 2017-03-28 | 2017-07-21 | 安徽中煦环保新材料科技有限公司 | A kind of forestry soil restoration of the ecosystem agent and preparation method thereof |
| CN107955616A (en) * | 2017-10-31 | 2018-04-24 | 浦江县合洪园艺研发有限公司 | A kind of preparation method suitable for harmful microorganism soil conditioner |
| CN108003879A (en) * | 2017-10-31 | 2018-05-08 | 浦江县合洪园艺研发有限公司 | A kind of modifying agent for chemical pollution soil |
| CN109385282B (en) * | 2018-10-30 | 2023-07-07 | 中国地质环境监测院 | Composition for repairing arsenic-phenanthrene composite contaminated soil and application thereof |
| CN111248227A (en) * | 2020-02-03 | 2020-06-09 | 湖北大学 | Modifying agent, preparation method thereof and method for promoting magnesium absorption of underground part of crop |
| CN111234828A (en) * | 2020-02-03 | 2020-06-05 | 南阳师范学院 | Passivating agent, preparation method and method for inhibiting cadmium absorption of overground part of crop |
| CN111187123A (en) * | 2020-02-03 | 2020-05-22 | 南阳师范学院 | Modifying agent, preparation method and method for promoting calcium absorption of crops in acid soil |
| CN112251238A (en) * | 2020-10-28 | 2021-01-22 | 武汉市秀谷科技有限公司 | Microbial preparation for farmland heavy metals and preparation method thereof |
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