CN102580045A - Application of intestine-consolidating capsules to preparation of medicines for reducing blood viscosity and inhibiting thrombopoiesis - Google Patents

Abstract

The invention relates to application of intestine-consolidating capsules to the preparation of medicines for reducing blood viscosity and inhibiting thrombopoiesis. The blood circulation promoting effect of the intestine-consolidating capsules is observed by experimental models of the intestine-consolidating capsules on the hemarheology of cold coagulation and blood stasis mice and the thrombopoiesis of the blood stasis mice; and pharmacodynamic experimental results indicate that 0.81 gram or 1.62 grams of intestine-consolidating capsules in each kilogram of crude medicines can reduce the blood viscosity of the mice inordinately and prolong the time of thrombopoiesis. The application opens up a bright prospect for the clinical application of the intestine-consolidating capsules.

Description

Guchang capsule suppresses the application in the thrombosis medicine at preparation treatment blood viscosity lowering

Technical field

The invention belongs to the Chinese medicine preparation technical field, relate to the new purposes of Chinese medicine, the Guchang capsule of saying so more specifically suppresses the application in the thrombosis medicine at preparation treatment blood viscosity lowering.

Background technology

Thrombosis originally is the very important self-protective mechanism of human body, is bearing the hemostatic function.When our health suffered wound and be hemorrhage, will rely on thrombosis stopped blooding, and this hemostasis thrombosis generally occurs in outside the blood vessel.But, if thrombosis occurs in the blood vessel, will influence blood circulation, the artery-clogging chamber causes that vascular flow obviously reduces even interruption fully, produces disease thus.Thrombosis is a kind of process that relates to the multifactor variation of many h and E factors interact with each other.The common clinically patient to thrombosis quality, topmost characteristics are that family history is arranged, repeated relapsing, youth, the seriousness of outbreak symptom and thrombosis position unusual.

Blood viscosity is the abbreviation of blood viscosity, is one of index of reflection viscosity of blood.Blood viscosity is high, hyperlipidemia, and flow velocity slows down, and lipid just is deposited on the inwall of blood vessel in the blood like this, causes luminal stenosis, blood supply insufficiency, causes the generation of diseases such as myocardial ischemia, cerebral thrombosis, extremity vascular thrombosis.

Guchang capsule is the Shi Dianbang chief physician's of Xiyuan Hospital, Chinese Medicine Academy of China clinical experience side; Having loose bowels is common clinically a kind of disease; Its pathological changes is mainly at liver,spleen,kidney and stomach, intestine and small intestine, normal lack of proper care with being invaded by exogenous pathogen, injury due to diet, temperament and interest and factors such as internal organs weakness relevant.Its main pathogenesis is the liver,spleen,kidney functional disorder, dysfunction of the spleen in transportation, and water-damp not being transformed is walked large intestine, acute diarrhea down; Wrong treatment is controlled in mistake, and state of an illness protracted course of disease, so that damaging the spleen and stomach burnt (taste) more involve the part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels (Liver and kidney), deficiency of spleen-YANG and kidneyYANG and give birth to cold; Temperature is lost and solid by following unit, so the clear rare or aqueous-grainy diarrhea of stool, very then slippage can't help, and it is stagnant that turbid damp accumulates, and QI and blood is stopped up resistance and freely then do not suffered from abdominal pain; Borborygmus, turbid damp dyspepsia are long-pending for a long time not to remove, strongly fragrant heat-transformation of a specified duration, then visible coldheat complex, the double card of seeing of deficiency and excess.To the primary disease characteristics, to control headed by sour and astringent solid intestinal and be engaged in, and be aided with the antidiarrheal of damming of closing the door, warming the interior is supporing yang with cold expelling, and heat clearing and damp drying is with hard intestinal, and promoting flow of QI and blood is double regulates the flow of vital energy.

Guchang capsule is made up of Halloysitum Rubrum, Galla Chinensis, Fructus Chebulae, Fructus Mume, Rhizoma Zingiberis, Semen Myristicae, Fructus Evodiae, Fructus Zanthoxyli, Cortex Cinnamomi, Rhizoma Coptidis, Cortex Phellodendri, Cortex Magnoliae Officinalis, Rhizoma Chuanxiong, Concha Ostreae, Massa Medicata Fermentata etc., has intestine-stabling and anti-diarrheal, the dispel function of the hot QI and blood regulating of cold expelling.Be used for the chronic diarrhea person that irritable bowel syndrome shows as coldheat complex, deficient and excessive syndromes appearing together.Disease is seen: it is rare clearly to defecate, or accompanies a little sticking in vain freezing, or aqueous-grainy diarrhea, and very then slippage can't help, stomachache borborygmus, aversion to cold and cold limbs, soreness of the waist and knees.Treat chronic diarrhea, coldheat complex, deficient and excessive syndromes appearing together card clinically effectively.But about the effect of invigorating blood circulation of Guchang capsule, particularly suppress the application in the thrombosis medicine, do not appear in the newspapers as yet at preparation treatment blood viscosity lowering.

Summary of the invention

The invention discloses Guchang capsule and suppress the application in the thrombosis medicine at preparation treatment blood viscosity lowering, wherein the consumption of Guchang capsule is: 0.81 or 1.62g/kg crude drug.

Consisting of of Guchang capsule: Halloysitum Rubrum(calcined) 500g, Rhizoma Coptidis 250g, Cortex Phellodendri 250g, Fructus Chebulae (pitted) 250g; Semen Myristicae 250g processs Cortex Magnoliae Officinalis 250g, Massa Medicata Fermentata 250g, Fructus Evodiae (processed) 200g; Cortex Cinnamomi 200g, Rhizoma Zingiberis 250g, Pericarpium Zanthoxyli 200g, Rhizoma Chuanxiong 200g; Concha Ostreae (calcined) 200g, Galla Chinensis 250g, Fructus Mume (core removed), 250g; Above ten five tastes crude drugs are pressed the ratio compatibility of recipe quantity, selected roguing thing, after silt was removed in washing, oven dry was ground into fine powder, and mixing adds suitable quantity of water, the system granule, drying, granulate divides the capsule of packing into, promptly gets.

The side of Guchang capsule separates as follows:

Fructus Mume is sour and astringent in the side, and relieving diarrhea with astringents is used for the chronic diarrhea chronic dysentery.The Rhizoma Coptidis bitter cold, heat clearing and damp drying, Galla Chinensis relieving diarrhea with astringents, a heat clearing away one antidiarrheal, giving consideration to both the incidental and fundamental.The hot warming spleen and stomach for dispelling cold of Rhizoma Zingiberis is used for the middle-jiao yang, function of the spleen and stomach deficiency and coldness that the chronic diarrhea chronic dysentery produces, but Rhizoma Coptidis (processed) is cold in addition, the Cortex Cinnamomi promoting the circulation of QI to relieve pain, and strengthening the spleen to promote digestion, the pain relieving of Rhizoma Chuanxiong blood-activating and qi-promoting, the stomachache that can alleviate the QI and blood stasis is coerced, and is adjuvant drug altogether.Put compatibility entirely, receive regulating the liver and spleen altogether, the merit of astringing intestine to stop diarrhea pain relieving.

The present invention observes the drug action of Guchang capsule through the research to the cold blood stasis aspect.

Method: adopt Guchang capsule that cold blood stasis hemorheology of rat, blood stasis rat suppository are formed experimental model, observe the effect of invigorating blood circulation of Guchang capsule;

The result: Guchang capsule 0.81 or 1.62g crude drug/kg can reduce rat blood viscosity to some extent, prolong TFT.

Conclusion: Guchang capsule has tangible function of promoting blood circulation to disperse blood clots, significantly reduces the thrombotic time.

The specific embodiment

Below in conjunction with embodiment the present invention is described; The scheme of embodiment described here; Do not limit the present invention; One of skill in the art can make improvements and change according to spirit of the present invention, and described these improvement and variation all should be regarded as within the scope of the invention, and scope of the present invention and essence are limited claim.Wherein said various medical material unless otherwise indicated, other all has commercially available.

Embodiment 1

Halloysitum Rubrum(calcined) 500g, Rhizoma Coptidis 250g, Cortex Phellodendri 250g, Fructus Chebulae (pitted) 250g, Semen Myristicae 250g processs Cortex Magnoliae Officinalis 250g; Massa Medicata Fermentata 250g, Fructus Evodiae (processed) 200g, Cortex Cinnamomi 200g, Rhizoma Zingiberis 250g, Pericarpium Zanthoxyli 200g; Rhizoma Chuanxiong 200g, Concha Ostreae (calcined) 200g, Galla Chinensis 250g, Fructus Mume (core removed), 250g; Above ten five tastes crude drugs are pressed the ratio compatibility of recipe quantity, selected roguing thing, after silt was removed in washing, oven dry was ground into fine powder, and mixing adds suitable quantity of water, the system granule, drying, granulate divides the capsule of packing into, promptly gets.

Embodiment 2

1 experiment purpose

Through research, observe the drug action of Guchang capsule, and inquire into the possible mechanism of action, for more wide prospect is opened up in the clinical practice of Guchang capsule to aspects such as colitis, chronic diarrhea, active chronic inflammation, cold blood stasis, gastrointestinal motility, immunomodulating.

2 experiment materials

2.1 experiment medicine and reagent

(1) receives reagent: Guchang capsule, lot number J002 (per 1 gram content is equivalent to crude drug 1 gram)

(2) other medicines and reagent

Heart pill of Musk: 22.5mg/ ball, Hehuang Pharmaceutical Co., Ltd., Shanghai, lot number: 100103.

Formalin: Tianjin Fengchuan Chemical Reagent Science & Technology Co., Ltd., lot number: 2010.02.25.

2,4 dinitrophenyl hydrazine: the import packing, valid until: 2012.12.

Adrenalin hydrochloride injection: Tianjin gold credit aminoacid company limited, lot number 1006231.

Fecal occult blood (FOB) diagnostic kit (duplex practice): lot number 201101251.

The rat leukocyte plain 6 enzyme immunoassay test kits that are situated between: Tianjin Hao ocean biological product science and technology limited Company, lot number 201106.

The rat leukocyte plain 8 enzyme immunoassay test kits that are situated between: Tianjin Hao ocean biological product science and technology limited Company, lot number 201106.

2.2 laboratory animal

Wistar rat: SPF level, Beijing Vital River Experimental Animals Technology Co., Ltd., credit number SCXK (capital) 2006-0009.

2.3 experimental apparatus

LG-R-80C type blood viscosity instrument: diligent generation Supreme Being's scientific instrument company limited in Beijing.

TU-1810 ultraviolet-uisible spectrophotometer: the general all purpose instrument company limited of analysing in Beijing.

The experimental thrombus in vivo of BT87-3 type forms analyzer: the development of medical college cardiovascular research chamber, packet header.

3. experimental technique and result

3.1 Guchang capsule is to the influence experiment of cold blood stasis

(1) Guchang capsule is to the influence experiment of cold blood stasis hemorheology of rat

Select 60 of healthy Wistar rats for use; Male and female half and half, body weight 200-220g is divided into 6 groups at random; Every group 10; That is: blank group, model control group, Guchang capsule 1.62g crude drug/kg, 0.81g crude drug/kg, three dose groups of 0.405g crude drug/kg and positive drug Heart pill of Musk 0.05g ball weight/kg group.Each group is gastric infusion respectively, administration volume 10ml/kg.Administration every day 1 time, continuous 7 days, blank and model control group gave equal-volume 0.5%CMC-Na, and other gives corresponding volume medicine.Behind the administration every day 1h; Except that the normal control group; All the other each groups all are placed on the 30min that swims in the frozen water, test the anesthesia of the 7th day swimming pneumoretroperitoneum injection 40mg/kg pentobarbital, abdominal aortic blood; With 3.8% sodium citrate anticoagulant (anticoagulant is 1:9 with the ratio of whole blood), measure WBV with LG-R-80C type blood viscosity instrument.

Table 1 Guchang capsule to the influence of cold blood stasis hemorheology of rat (

, n=10)

△ △ P < 0.01 (comparing) with the normal control group; * P 0.05, * * P 0.01 (with model group relatively)

Table 1 is the result show: model group each cut speed down blood viscosity significantly raise.Guchang capsule 1.62g crude drug/kg, 0.81g crude drug/kg can reduce rat blood viscosity to some extent, and be remarkable with the model control group comparing difference.Positive drug has similar effect.

(2) Guchang capsule is tested the influence that the blood stasis rat suppository forms

Select 72 of healthy Wistar rats for use; Male and female half and half, body weight 200-220g is divided into 6 groups at random; Every group 12; That is: normal control group, model control group, Guchang capsule 1.62g crude drug/kg, 0.81g crude drug/kg, three dose groups of 0.405g crude drug/kg and positive drug Heart pill of Musk 0.05g ball weight/kg group.Each group is gastric infusion respectively, administration volume 10ml/kg.Administration every day 1 time, continuous 7 days, blank and model control group gave equal-volume 0.5%CMC-Na, and other gives corresponding volume medicine.Tested the 6th day, behind the administration 1h, except that the normal control group, all the other respectively organize equal subcutaneous injection 0.1% adrenalin hydrochloride 0.8mg/kg, and the 5min that swims in the 3h frozen water of injection back injects equivalent 0.1% adrenalin hydrochloride once more behind the 3h.Fasting 12h before the administration in the 7th day; 1h lumbar injection 40mg/kg pentobarbital anesthesia after the administration; Separate the about 15mm of a side common carotid artery, on common carotid artery, continue to stimulate blood vessel wall 7min with the electric current of 1.5mA with the stimulating electrode on the thrombosis appearance and temperature sensor hook; Close the thorn energizing switch then, temperature sensor monitors blood vessel surface variations in temperature.When the thrombosis plug flow, blood vessel distal end temperature bust, instrument is reported to the police.From stimulation begin to time of fire alarming as TFT.

Table 2 Guchang capsule to the influence of blood stasis rat suppository formation time (

, n=12)

△ △ P < 0.01 (comparing) with the normal control group; * P 0.05 (with model group relatively)

Table 2 is the result show: the model group TFT significantly reduces.Guchang capsule 1.62g crude drug/kg, 0.81g crude drug/kg can prolong TFT to some extent, and be remarkable with the model control group comparing difference.Positive drug has similar effect.

Claims (2)

1. Guchang capsule suppresses the application in the thrombosis medicine at preparation treatment blood viscosity lowering, and Guchang capsule consumption wherein is: 0.81g crude drug/kg or 1.62g crude drug/kg.

2. the described application of claim 1, Guchang capsule wherein refer to remove the content behind the softgel shell.