Summary of the invention
It is beautiful forever 208 that technical problem to be solved by this invention provides a kind of high yield, stable yields, high-quality, high black grain corn hybrid seed anti-, wide adaptability, in order to overcome the deficiency that exists in the prior art.
The present invention solves the overall technical architecture that its technical problem adopts:
A kind of black grain corn hybrid seed is beautiful 208 breeding method forever, specifically may further comprise the steps:
A. the seed selection of the black grain of horse flute profile corn inbred line black 151
To deceive glutinous No. 3 is basic material, and with 478 hybridization of horse flute profile yellow corn inbred line, its first-filial generation F1 breeds the black corn inbred line black 151 of horse flute profile through five separation screenings of selfing from generation to generation;
B. the seed selection of the black grain of osculant corn inbred line black 231
To deceive glutinous No. 3 is basic material, and with 5237 hybridization of solids type yellow corn inbred line, its first-filial generation F1 breeds the black corn inbred line black 231 of osculant through five separation screenings of selfing from generation to generation;
C. the horse flute profile black grain corn inbred line seed selection of H83 forever
Is background material with the black grain of horse flute profile corn inbred line black 151 with horse flute profile yellow corn inbred Zheng 58, breeds the black grain of horse flute profile corn inbred line H83 forever;
D. the osculant black grain corn inbred line seed selection of H52 forever
Is background material with the black grain of osculant corn inbred line black 231 with the prosperous 7-2 of solids type yellow corn inbred line, breeds the black grain of osculant corn inbred line H52 forever;
E. beautiful 208 the seed selection forever of black grain corn hybrid seed
So that H83 inbred line work is maternal forever, the H52 inbred line is made male parent forever, and black grain corn hybrid seed jade 208 is forever bred in hybridization.
Concrete technical scheme of the present invention also has:
Black glutinous No. 3 and horse flute profile yellow corn inbred line 478 hybridization back generation first-filial generation F1 among the said step a; Select for use the black grain of the non-glutinous matter fruit ear seed among the F1 to do kind of a continuous selfing again; Wherein each self-generation all selects for use the black grain of the non-glutinous matter fruit ear seed of a generation to plant; Cultivate through the selfing of five generations, breed the black grain of horse flute profile corn inbred line black 151.
Black glutinous No. 3 and solids type yellow corn inbred line 5237 hybridization back generation first-filial generation F1 among the said step b; Select for use the black grain of the non-glutinous matter fruit ear seed among the F1 to do kind of a continuous selfing again; Wherein each self-generation all selects for use the black grain of the non-glutinous matter fruit ear seed of a generation to plant; Cultivate through the selfing of five generations, breed the black grain of osculant corn inbred line black 231.
The black grain of horse flute profile corn inbred line black 151 generates first-filial generation F1 with horse flute profile yellow corn inbred Zheng 58 hybridization backs among the said step c; Choosing black grain fruit ear seed among the F1, to do after kind of the selfing generation be that the parent backcrosses with Zheng 58 again; The black grain fruit ear seed choosing again in the kind of backcrossing is done kind of a continuous selfing; Wherein each self-generation all selects for use the black grain fruit ear seed of a generation to plant, and through six separation screenings of selfing from generation to generation, breeds the black corn inbred line of horse flute profile H83 forever.
The black grain of osculant in said steps d corn inbred line black 231 generates first-filial generation (F1) with the prosperous 7-2 hybridization of solids type yellow corn inbred line back; Choosing black grain fruit ear seed among the F1, to do after kind of the selfing generation be that the parent backcrosses with prosperous 7-2 again; The black grain fruit ear seed choosing again in the kind of backcrossing is done kind of a continuous selfing; Wherein each self-generation all selects for use the black grain fruit ear seed of a generation to plant, and through six separation screenings of selfing from generation to generation, breeds the black corn inbred line of osculant H52 forever.
The female parent of the present invention principal character of H83 forever is following:
About summer sowing breeding time 90 days, the seedling leaf sheath is green, and plant type is compact, 160 centimetres of plant heights, 60 centimetres of fringe positions; 21 leaves of complete stool, 5~10 of tassel branches, flower pesticide is yellow, and female fringe bract is moderate, and filigree is dark green; The fruit ear cylinder type, 16 centimetres of spike lengths, 12~14 row grains, cob black, the axle center is red; Grain black, horse flute profile, anti-corn southern leaf blight, leaf blight, smut and bacterial wilt, high resistance to fold.
The male parent of the present invention principal character of H52 forever is following:
About summer sowing breeding time 89 days, show purple during the seedling leaf sheath is green slightly, the plant type hemicompact is gathered, 162 centimetres of plant heights, 85 centimetres of fringe positions; 21 leaves of complete stool, 14~19 of tassel branches, flower pesticide is yellow, and female fringe bract is moderate; Filigree is dark green, fruit ear short cylinder type, 10.2 centimetres of spike lengths, thick 4.3 centimetres of fringe; 16 row grains, seed is tight, cob black, the axle center is red.Grain dark (length) black, osculant, anti-corn southern leaf blight, bacterial wilt and rough dwarf disease.
Beautiful forever 208 principal characters that formed by this method cultivation are following:
About summer sowing breeding time 103 days, seedling leaf sheath purple, plant type is compact, 256 centimetres of plant heights, 100 centimetres of fringe positions, 21 leaves of complete stool, 13~16 of tassel branches, flower pesticide is yellow, and filigree is dark green.The fruit ear cylinder type, 16.4 centimetres of spike lengths, tassel row number 16 row, seed-producing rate 87%, cob black, the axle center is red.Grain black, horse flute profile, thousand kernel weight 328 grams.Identify through Inst. of Plant Protection, Hebei-Prov. Academy of Agricultural and Forestry Scie inoculation, anti-gall smut, short mosaic disease, in anti-helminthosporium maydis, high resistance to fold, the straw of living are ripe.
The technique effect that the present invention obtained is:
(1) high yield, stable yields, the summer in 2007, our company produced than test, 598.2 kilograms of average yield per mus; Comparison is according to kind of Zheng Dan 958 raising the output 3.6%; 2008~2009 years low close group of multiple spot regional tests of Hebei province's summer corn, 572~594 kilograms of average yield per mus are than 471 kilograms of the black glutinous No. 3 multiple spots test average yield per mus in Shandong; Mu increases 101~173 kilograms, raising the output 21%; 2010, the test of Hebei province's Summer Maize Production, 563 kilograms of average yield per mus.
(2) grain quality is good, and is nutritious, edible favourable health, feeding favourable raising domestic birds and animals rate of body weight gain.Beautiful forever 208 (the inspection declaration name H211 of jet rice of the present invention; After name be forever beautiful 208) detect through the analysis room, Beijing City Nutrient Source Inst with Zheng Dan 958 and relatively; Generally higher (the detection foundation: GB/T 18246-2000) of beautiful forever 208 amino acid contents; Especially higher to the necessary lysine content of human body, improve 37.5% than Zheng Dan 958, it has, and the human calcium of promotion absorbs, prevents osteoporosis, increases hemochrome, does aging, multiple function such as build up health; Also have multiple efficacies such as the domestic birds and animals of increasing appetite, weightening finish, raising meat, specifically see table 1; Beautiful forever 208 main trace element generally are higher than Zheng Dan 958; Especially calcium, Se content are high, and calcium is that human body institute is necessary, and selenium is to removing body's cholesterol, getting rid of toxin, enhancing human immune etc. good effect is all arranged; Be referred to as " anticancer king " by people, specifically see table 2,3,4.
Table 1 is beautiful (H211) and the main amino acid comparison sheet of yellow maize (Zheng Dan 958) forever
Table 2 H211 corn (jade 208 forever) is examined then result
Table 3 Zheng Dan 958 corns are examined then result
Table 4 is beautiful (H211) and yellow maize (Zheng Dan 958) main trace element comparison sheet forever
(3) disease-resistant, lodging resistance good.Identify through Inst. of Plant Protection, Hebei-Prov. Academy of Agricultural and Forestry Scie, anti-gall smut, short mosaic disease, in anti-helminthosporium maydis; In addition, beautiful forever 208 plant type structures of the present invention are reasonable, and the fringe position is lower, has extremely strong lodging resistance.
(4) breeding technique is simple, and the seed production cost is low, and adaptability is wider, is suitable for large scale application.
Embodiment
Below will combine accompanying drawing and embodiment that the present invention is further described:
A kind of black grain corn hybrid seed is beautiful 208 breeding method forever, specifically may further comprise the steps:
A. the seed selection of the black grain of horse flute profile corn inbred line black 151
Black waxy corn single cross hybrid in 1998 is bought by Changwei seeds company for black glutinous No. 3, and this kind is bred by agricultural school, Changwei, Shandong Province, and nineteen ninety-five is assert through the Shandong Province.Make female parent with black glutinous No. 3 the same year, and the horse flute profile yellow corn inbred line 478 that effluent north seeds company obtains is made male parent, breeds black glutinous No. 3 * 478 crossbreed.The division headquarters farm sowing of black glutinous No. 3 * 478 first-filial generations (F1) of winter in 1998 in Sanya, Hainan; Delegation's 15 strains; Flowering stage whole plant female fringe and tassel bagging, the artificial pollination selfing generates selfing generation fruit ear seed; Ripe consequence tassel seed color has two kinds of fruit ears of black grain and yellow grain, but does not have rule.According to the field performance, from the first-filial generation head progeny row, choose the black grain of three non-glutinous matter of selfing generation fruit ear, dry threshing and plant.Work kind seed summer sowing with the black grain of three non-glutinous matter of selfing generation fruit ear in 1999 in the experimental field, the seed of every fringe is broadcast 1 row, and selfing is also all carried out in every row 19 strains, generates selfing two generations fruit ear seed, and the black grain in ripe back is 1: 1 with yellow grain fruit ear and separates.According to plant and the performance of fringe portion, from black grain fruit ear selfing generation head progeny row, choose the black grain of three selfing non-glutinous matter of two generations fruit ear, dry threshing and plant.Thereafter,, still have a small amount of yellow grain fruit ear to occur, but do not have rule from accompanying each other generation by last method, to the 5th generation of selfing, the fruit ear that delegation is arranged all be the black grain of a non-glutinous matter fruit ear, breeds the black corn inbred line of horse flute profile and deceives 151.
B. the seed selection of the black grain of osculant corn inbred line black 231
1998 maternal with black glutinous No. 3 works, makes male parent with the solids type yellow corn inbred line 5237 that effluent north seeds company obtains, and breeds black glutinous No. 3 * 5237 crossbreed.The division headquarters farm sowing of black glutinous No. 3 * 5237 first-filial generations (F1) of winter in 1998 in Sanya, Hainan; Delegation's 15 strains; Flowering stage whole plant female fringe and tassel bagging, the artificial pollination selfing generates selfing generation fruit ear seed; Ripe consequence tassel seed color has two kinds of fruit ears of black grain and yellow grain, but does not have rule.According to the field performance, from the first-filial generation head progeny row, choose the black grain of three non-glutinous matter of selfing generation fruit ear, dry threshing and plant.Work kind seed summer sowing with the black grain of three non-glutinous matter of selfing generation fruit ear in 1999 in the experimental field, the seed of every fringe is broadcast 1 row, and selfing is also all carried out in every row 19 strains, generates selfing two generations fruit ear seed, and the black grain in ripe back is 1: 1 with yellow grain fruit ear and separates.According to plant and the performance of fringe portion, from black grain fruit ear selfing generation head progeny row, choose the black grain of three selfing non-glutinous matter of two generations selfing fruit ear, dry threshing and plant.Thereafter,, still have a small amount of yellow grain fruit ear to occur, but do not have rule from accompanying each other generation by last method, to the 5th generation of selfing, the fruit ear that delegation is arranged all be the black grain of a non-glutinous matter fruit ear, breeds the black corn inbred line of osculant and deceives 231.
C. the horse flute profile black grain corn inbred line seed selection of H83 forever
Winter in 2002 was done maternal with black 151 in Hainan, the yellow corn inbred Zheng 58 that kind industry company of effluent south Jinboshi obtains is made male parent, is made into black 151 * Zheng, 58 first-filial generations (F1).
Summer in 2003 is in black 151 * Zheng, 58 first-filial generations (F1), 1 row district's 19 strains of our company's experimental field sowing; Whole selfings in flowering stage; Generate selfing generation fruit ear seed, the fruit ear seed color after the maturation has two kinds of fruit ears of black grain and yellow grain, but does not have rule; Choose the black grain of 3 selfing generation fruit ear, dry threshing respectively and plant.
2003 winters, the work kind seed of the black grain of 3 selfing generation fruit ear is respectively broadcast delegation's (15 strain) in Hainan, all plant makes maternal (ripe fruit ear seed color; Black grain is 1: 1 with yellow grain fruit ear and separates); Zheng 58 makes male parent, backcrosses, and forms a selfing generation * Zheng 58 kind of backcrossing; Choose three black fruit ears of backcrossing after the maturation, dry threshing respectively and plant.
Summer in 2004; The seed of three black kind of fruit ears of backcrossing is respectively broadcast delegation's (19 strain) in our company experimental field; Whole plant selfings in flowering stage; Generation has the selfing generation fruit ear (black grain does not still have rule with the quantity of yellow grain fruit ear) of black grain and yellow grain, chooses three black grain selfing generation fruit ears from kind of the head progeny row of backcrossing, and dries threshing respectively and plants.
Winter in 2004; Three black grain selfing generation fruit ear seeds of sowing in Hainan, every tassel seed is broadcast delegation, and flowering stage, whole plant carried out the selfing second time; Generation has the selfing two generations fruit ear (black grain is 1: 1 with yellow grain fruit ear and separates) of black grain and yellow grain; Choose three black grain selfing two generations fruit ears, dry threshing and plant, supply next to continue selfing choosing system from generation to generation.According to the method described above, altogether through six separation screenings of selfing from generation to generation, breed the black grain of horse flute profile corn inbred line H83 forever in the summer in 2006.
D. the osculant black grain corn inbred line seed selection of H52 forever
Winter in 2002 was done maternal with black 231 in Hainan, the prosperous 7-2 of yellow corn inbred line that kind industry company of effluent south Jinboshi obtains makes male parent, is made into black 231 * prosperous 7-2 first-filial generation (F1).
Summer in 2003 is in black 231 * prosperous 7-2 first-filial generation (F1), 1 row district's 19 strains of our company's experimental field sowing; Whole selfings in flowering stage; Generate selfing generation fruit ear seed, the fruit ear seed color after the maturation has two kinds of fruit ears of black grain and yellow grain, but does not have rule; Choose the black grain of 3 selfing generation fruit ear, dry threshing respectively and plant.
2003 winters; The seed of the black grain of 3 selfing generation fruit ear is respectively broadcast delegation's (15 strain) in Hainan, all plant does maternal (the black grain of the fruit ear seed color after the maturation is 1: 1 with yellow grain fruit ear and separates), and prosperous 7-2 makes male parent; Backcross; Form a selfing generation * prosperous 7-2 kinds of backcrossing, choose three black grains fruit ears of backcrossing after the maturation, dry and distinguish threshing and plant.
Summer in 2004; Three black kind of fruit ear seeds of backcrossing are respectively broadcast delegation's (19 strain) in our company experimental field; Whole plant selfings in flowering stage; Generation has the selfing generation fruit ear (black grain does not still have rule with the quantity of yellow grain fruit ear) of black grain and yellow grain, chooses three black grain selfing generation fruit ears from kind of the head progeny row of backcrossing, and dries threshing respectively and plants.
Winter in 2004; The seed of three black grain selfing generation fruit ears of sowing in Hainan, the seed of every fringe is broadcast delegation, and flowering stage, whole plant carried out the selfing second time; Generation has the selfing two generations fruit ear (black grain is 1: 1 with yellow grain fruit ear and separates) of black grain and yellow grain; Choose three black grain selfing two generations fruit ears, dry threshing and plant, supply next to continue selfing choosing system from generation to generation.According to the method described above, altogether through six separation screenings of selfing from generation to generation, breed the black grain of horse flute profile corn inbred line H52 forever in the summer in 2006.
E. beautiful 208 the seed selection forever of black grain corn hybrid seed
Winter in 2006 was female parent in Hainan with H83 forever, was male parent with H52 forever; The temporary called after H211 of black grain corn hybrid seed is bred in hybridization, and the summer in 2007 was carried out the output comparative test in our unit, 598.2 kilograms of average yield per mus; Comparison was according to kind of Zheng Dan 958 raising the output 3.6%, and the economical character performance was better promoted Hebei province's corn variety preliminary experiment in 2008; Kind name H211 names and is forever beautiful 208; Pressed special corn through the authorization of the Hebei province variety certification committee in 2011 through Hebei province's corn regional test and production test in 2010, authorization is numbered the Ji and examines beautiful No. 2011005.
Of the present invention kind of method for planting characteristics are: suitable dense planting, and planting density 4000-4500 strain ∕ mu, field management needs the heavy dressing jointing fertile, takes out and need water sufficient water before male, and the fruit ear flavescence can be gathered in the crops, if results evenings, plants skin and has the be full of cracks Signs of Mould that absorbs water.