CN102577935A - Breeding method for black-kernel hybrid corn seeds Yongyu 208 - Google Patents

Breeding method for black-kernel hybrid corn seeds Yongyu 208 Download PDF

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CN102577935A
CN102577935A CN2012100854366A CN201210085436A CN102577935A CN 102577935 A CN102577935 A CN 102577935A CN 2012100854366 A CN2012100854366 A CN 2012100854366A CN 201210085436 A CN201210085436 A CN 201210085436A CN 102577935 A CN102577935 A CN 102577935A
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black
generation
grain
inbred line
corn
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CN102577935B (en
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崔绍平
吴涛
白金芳
邢树良
燕利彬
李建国
徐士磊
杨国平
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Beijing Pu Tai Agricultural Development Co., Ltd.
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HEBEI JINAN SEED INDUSTRY Co Ltd
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Abstract

The invention relates to a breeding method for black-kernel hybrid corn seeds Yongyu 208. Specifically, the breeding method comprises the steps of: breeding black 151 by taking black glutinous No.3 and a yellow-kernel corn self-bred line 478 as background materials, breeding black 231 by taking the black glutinous No.3 and a yellow-kernel corn self-bred line 5237 as background materials, breeding a black-kernel corn self-bred line Yong H83 by taking the black-kernel corn self-bred line black 151 and a yellow-kernel corn self-bred line Zheng 58 as background materials, breeding a black-kernel corn self-bred line Yong H52 by taking the black-kernel corn self-bred line black 231 and a yellow-kernel corn self-bred line Chang 7-2 as background materials, and finally breeding the black-kernel hybrid corn seeds Yongyu 208 by taking Yong H83 as female parents and Yong H52 as male parents. The breeding method for the black-kernel hybrid corn seeds Yongyu 208 has the advantages that the yield is high and stable, the nutrients of grains are rich, the content of minerals, trace elements, amino acid and the like of the black-kernel hybrid corn seeds Yongyu 208 is much higher than the content of minerals, trace elements, amino acid and the like of common corns, the human body health is promoted by eating the black-kernel hybrid corn seeds Yongyu 208, the growth and the development of livestock and poultry are facilitated by using the black-kernel hybrid corn seeds Yongyu 208 as feed, the black-kernel hybrid corn seeds Yongyu 208 are lodging-resistant and disease-and-pest-resistant and the popularization and application scope is wide.

Description

A kind of black grain corn hybrid seed is beautiful 208 breeding method forever
Technical field
The present invention relates to a kind of breeding method of corn variety, particularly a kind of black grain corn hybrid seed is beautiful 208 breeding method forever.
Background technology
Corn accounts for more than 65% of world's coarse food grain output, accounts for 90% of China's coarse food grain output, also is simultaneously the main raw material of making mixed feed, how to combine existing kind, and cultivating better corn variety is the key subjects that China agricultural science and technology worker faces.Black grain corn is claimed jet rice again, is a mutation in the maize culture, originates from American continent equally with conventional corn.Black grain milpa phenotype is identical with conventional corn, is characterized in that grain is black, and axle is black, the heart red (the cob heart), and seed is nutritious.According to " plantation of a jet rice and processing and utilization " book (Zhang Xiaomei: 2002. Beijing Golden Shield publishing houses 2002.79); The nutrient component of jet rice is apparently higher than yellow maize; Protein, amino acid, fat and coarse-fibred content improve 10.9% respectively than yellow maize; More than 30.3%, 56.6% and 6.1%; The content of the necessary trace elements iron of human body, zinc, calcium, copper, manganese exceeds 17~80% than yellow maize respectively, and the content that especially has the selenium of anti-cancer, antitumaous effect is higher than the content of yellow maize far away.2002. Golden Shield publishing houses) according to " special corn improved seeds and culture technique " book (Huang Changling etc.: bibliographical information such as; Lysine is the necessary amino acid of people and nonruminant; Utilization contains the high corn of lysine domestic animals and fowls such as feed pigs, and its rate of body weight gain can significantly improve.Therefore, the black grain of research and development corn has important function to uplifting the people's living standard, building up people's health.At present more domestic R&D institutions begin research and development utilization to a black grain corn in succession, but its kind all exists that output is on the low side, accommodation has limitation, disease and insect resistance and lodging tolerance are poor, can not large scale application etc. defective.
Summary of the invention
It is beautiful forever 208 that technical problem to be solved by this invention provides a kind of high yield, stable yields, high-quality, high black grain corn hybrid seed anti-, wide adaptability, in order to overcome the deficiency that exists in the prior art.
The present invention solves the overall technical architecture that its technical problem adopts:
A kind of black grain corn hybrid seed is beautiful 208 breeding method forever, specifically may further comprise the steps:
A. the seed selection of the black grain of horse flute profile corn inbred line black 151
To deceive glutinous No. 3 is basic material, and with 478 hybridization of horse flute profile yellow corn inbred line, its first-filial generation F1 breeds the black corn inbred line black 151 of horse flute profile through five separation screenings of selfing from generation to generation;
B. the seed selection of the black grain of osculant corn inbred line black 231
To deceive glutinous No. 3 is basic material, and with 5237 hybridization of solids type yellow corn inbred line, its first-filial generation F1 breeds the black corn inbred line black 231 of osculant through five separation screenings of selfing from generation to generation;
C. the horse flute profile black grain corn inbred line seed selection of H83 forever
Is background material with the black grain of horse flute profile corn inbred line black 151 with horse flute profile yellow corn inbred Zheng 58, breeds the black grain of horse flute profile corn inbred line H83 forever;
D. the osculant black grain corn inbred line seed selection of H52 forever
Is background material with the black grain of osculant corn inbred line black 231 with the prosperous 7-2 of solids type yellow corn inbred line, breeds the black grain of osculant corn inbred line H52 forever;
E. beautiful 208 the seed selection forever of black grain corn hybrid seed
So that H83 inbred line work is maternal forever, the H52 inbred line is made male parent forever, and black grain corn hybrid seed jade 208 is forever bred in hybridization.
Concrete technical scheme of the present invention also has:
Black glutinous No. 3 and horse flute profile yellow corn inbred line 478 hybridization back generation first-filial generation F1 among the said step a; Select for use the black grain of the non-glutinous matter fruit ear seed among the F1 to do kind of a continuous selfing again; Wherein each self-generation all selects for use the black grain of the non-glutinous matter fruit ear seed of a generation to plant; Cultivate through the selfing of five generations, breed the black grain of horse flute profile corn inbred line black 151.
Black glutinous No. 3 and solids type yellow corn inbred line 5237 hybridization back generation first-filial generation F1 among the said step b; Select for use the black grain of the non-glutinous matter fruit ear seed among the F1 to do kind of a continuous selfing again; Wherein each self-generation all selects for use the black grain of the non-glutinous matter fruit ear seed of a generation to plant; Cultivate through the selfing of five generations, breed the black grain of osculant corn inbred line black 231.
The black grain of horse flute profile corn inbred line black 151 generates first-filial generation F1 with horse flute profile yellow corn inbred Zheng 58 hybridization backs among the said step c; Choosing black grain fruit ear seed among the F1, to do after kind of the selfing generation be that the parent backcrosses with Zheng 58 again; The black grain fruit ear seed choosing again in the kind of backcrossing is done kind of a continuous selfing; Wherein each self-generation all selects for use the black grain fruit ear seed of a generation to plant, and through six separation screenings of selfing from generation to generation, breeds the black corn inbred line of horse flute profile H83 forever.
The black grain of osculant in said steps d corn inbred line black 231 generates first-filial generation (F1) with the prosperous 7-2 hybridization of solids type yellow corn inbred line back; Choosing black grain fruit ear seed among the F1, to do after kind of the selfing generation be that the parent backcrosses with prosperous 7-2 again; The black grain fruit ear seed choosing again in the kind of backcrossing is done kind of a continuous selfing; Wherein each self-generation all selects for use the black grain fruit ear seed of a generation to plant, and through six separation screenings of selfing from generation to generation, breeds the black corn inbred line of osculant H52 forever.
The female parent of the present invention principal character of H83 forever is following:
About summer sowing breeding time 90 days, the seedling leaf sheath is green, and plant type is compact, 160 centimetres of plant heights, 60 centimetres of fringe positions; 21 leaves of complete stool, 5~10 of tassel branches, flower pesticide is yellow, and female fringe bract is moderate, and filigree is dark green; The fruit ear cylinder type, 16 centimetres of spike lengths, 12~14 row grains, cob black, the axle center is red; Grain black, horse flute profile, anti-corn southern leaf blight, leaf blight, smut and bacterial wilt, high resistance to fold.
The male parent of the present invention principal character of H52 forever is following:
About summer sowing breeding time 89 days, show purple during the seedling leaf sheath is green slightly, the plant type hemicompact is gathered, 162 centimetres of plant heights, 85 centimetres of fringe positions; 21 leaves of complete stool, 14~19 of tassel branches, flower pesticide is yellow, and female fringe bract is moderate; Filigree is dark green, fruit ear short cylinder type, 10.2 centimetres of spike lengths, thick 4.3 centimetres of fringe; 16 row grains, seed is tight, cob black, the axle center is red.Grain dark (length) black, osculant, anti-corn southern leaf blight, bacterial wilt and rough dwarf disease.
Beautiful forever 208 principal characters that formed by this method cultivation are following:
About summer sowing breeding time 103 days, seedling leaf sheath purple, plant type is compact, 256 centimetres of plant heights, 100 centimetres of fringe positions, 21 leaves of complete stool, 13~16 of tassel branches, flower pesticide is yellow, and filigree is dark green.The fruit ear cylinder type, 16.4 centimetres of spike lengths, tassel row number 16 row, seed-producing rate 87%, cob black, the axle center is red.Grain black, horse flute profile, thousand kernel weight 328 grams.Identify through Inst. of Plant Protection, Hebei-Prov. Academy of Agricultural and Forestry Scie inoculation, anti-gall smut, short mosaic disease, in anti-helminthosporium maydis, high resistance to fold, the straw of living are ripe.
The technique effect that the present invention obtained is:
(1) high yield, stable yields, the summer in 2007, our company produced than test, 598.2 kilograms of average yield per mus; Comparison is according to kind of Zheng Dan 958 raising the output 3.6%; 2008~2009 years low close group of multiple spot regional tests of Hebei province's summer corn, 572~594 kilograms of average yield per mus are than 471 kilograms of the black glutinous No. 3 multiple spots test average yield per mus in Shandong; Mu increases 101~173 kilograms, raising the output 21%; 2010, the test of Hebei province's Summer Maize Production, 563 kilograms of average yield per mus.
(2) grain quality is good, and is nutritious, edible favourable health, feeding favourable raising domestic birds and animals rate of body weight gain.Beautiful forever 208 (the inspection declaration name H211 of jet rice of the present invention; After name be forever beautiful 208) detect through the analysis room, Beijing City Nutrient Source Inst with Zheng Dan 958 and relatively; Generally higher (the detection foundation: GB/T 18246-2000) of beautiful forever 208 amino acid contents; Especially higher to the necessary lysine content of human body, improve 37.5% than Zheng Dan 958, it has, and the human calcium of promotion absorbs, prevents osteoporosis, increases hemochrome, does aging, multiple function such as build up health; Also have multiple efficacies such as the domestic birds and animals of increasing appetite, weightening finish, raising meat, specifically see table 1; Beautiful forever 208 main trace element generally are higher than Zheng Dan 958; Especially calcium, Se content are high, and calcium is that human body institute is necessary, and selenium is to removing body's cholesterol, getting rid of toxin, enhancing human immune etc. good effect is all arranged; Be referred to as " anticancer king " by people, specifically see table 2,3,4.
Table 1 is beautiful (H211) and the main amino acid comparison sheet of yellow maize (Zheng Dan 958) forever
Table 2 H211 corn (jade 208 forever) is examined then result
Figure 2012100854366100002DEST_PATH_IMAGE003
Table 3 Zheng Dan 958 corns are examined then result
Table 4 is beautiful (H211) and yellow maize (Zheng Dan 958) main trace element comparison sheet forever
Figure 2012100854366100002DEST_PATH_IMAGE005
(3) disease-resistant, lodging resistance good.Identify through Inst. of Plant Protection, Hebei-Prov. Academy of Agricultural and Forestry Scie, anti-gall smut, short mosaic disease, in anti-helminthosporium maydis; In addition, beautiful forever 208 plant type structures of the present invention are reasonable, and the fringe position is lower, has extremely strong lodging resistance.
(4) breeding technique is simple, and the seed production cost is low, and adaptability is wider, is suitable for large scale application.
Description of drawings
Fig. 1 is a breeding line spectrogram of the present invention.
Embodiment
Below will combine accompanying drawing and embodiment that the present invention is further described:
A kind of black grain corn hybrid seed is beautiful 208 breeding method forever, specifically may further comprise the steps:
A. the seed selection of the black grain of horse flute profile corn inbred line black 151
Black waxy corn single cross hybrid in 1998 is bought by Changwei seeds company for black glutinous No. 3, and this kind is bred by agricultural school, Changwei, Shandong Province, and nineteen ninety-five is assert through the Shandong Province.Make female parent with black glutinous No. 3 the same year, and the horse flute profile yellow corn inbred line 478 that effluent north seeds company obtains is made male parent, breeds black glutinous No. 3 * 478 crossbreed.The division headquarters farm sowing of black glutinous No. 3 * 478 first-filial generations (F1) of winter in 1998 in Sanya, Hainan; Delegation's 15 strains; Flowering stage whole plant female fringe and tassel bagging, the artificial pollination selfing generates selfing generation fruit ear seed; Ripe consequence tassel seed color has two kinds of fruit ears of black grain and yellow grain, but does not have rule.According to the field performance, from the first-filial generation head progeny row, choose the black grain of three non-glutinous matter of selfing generation fruit ear, dry threshing and plant.Work kind seed summer sowing with the black grain of three non-glutinous matter of selfing generation fruit ear in 1999 in the experimental field, the seed of every fringe is broadcast 1 row, and selfing is also all carried out in every row 19 strains, generates selfing two generations fruit ear seed, and the black grain in ripe back is 1: 1 with yellow grain fruit ear and separates.According to plant and the performance of fringe portion, from black grain fruit ear selfing generation head progeny row, choose the black grain of three selfing non-glutinous matter of two generations fruit ear, dry threshing and plant.Thereafter,, still have a small amount of yellow grain fruit ear to occur, but do not have rule from accompanying each other generation by last method, to the 5th generation of selfing, the fruit ear that delegation is arranged all be the black grain of a non-glutinous matter fruit ear, breeds the black corn inbred line of horse flute profile and deceives 151.
B. the seed selection of the black grain of osculant corn inbred line black 231
1998 maternal with black glutinous No. 3 works, makes male parent with the solids type yellow corn inbred line 5237 that effluent north seeds company obtains, and breeds black glutinous No. 3 * 5237 crossbreed.The division headquarters farm sowing of black glutinous No. 3 * 5237 first-filial generations (F1) of winter in 1998 in Sanya, Hainan; Delegation's 15 strains; Flowering stage whole plant female fringe and tassel bagging, the artificial pollination selfing generates selfing generation fruit ear seed; Ripe consequence tassel seed color has two kinds of fruit ears of black grain and yellow grain, but does not have rule.According to the field performance, from the first-filial generation head progeny row, choose the black grain of three non-glutinous matter of selfing generation fruit ear, dry threshing and plant.Work kind seed summer sowing with the black grain of three non-glutinous matter of selfing generation fruit ear in 1999 in the experimental field, the seed of every fringe is broadcast 1 row, and selfing is also all carried out in every row 19 strains, generates selfing two generations fruit ear seed, and the black grain in ripe back is 1: 1 with yellow grain fruit ear and separates.According to plant and the performance of fringe portion, from black grain fruit ear selfing generation head progeny row, choose the black grain of three selfing non-glutinous matter of two generations selfing fruit ear, dry threshing and plant.Thereafter,, still have a small amount of yellow grain fruit ear to occur, but do not have rule from accompanying each other generation by last method, to the 5th generation of selfing, the fruit ear that delegation is arranged all be the black grain of a non-glutinous matter fruit ear, breeds the black corn inbred line of osculant and deceives 231.
C. the horse flute profile black grain corn inbred line seed selection of H83 forever
Winter in 2002 was done maternal with black 151 in Hainan, the yellow corn inbred Zheng 58 that kind industry company of effluent south Jinboshi obtains is made male parent, is made into black 151 * Zheng, 58 first-filial generations (F1).
Summer in 2003 is in black 151 * Zheng, 58 first-filial generations (F1), 1 row district's 19 strains of our company's experimental field sowing; Whole selfings in flowering stage; Generate selfing generation fruit ear seed, the fruit ear seed color after the maturation has two kinds of fruit ears of black grain and yellow grain, but does not have rule; Choose the black grain of 3 selfing generation fruit ear, dry threshing respectively and plant.
2003 winters, the work kind seed of the black grain of 3 selfing generation fruit ear is respectively broadcast delegation's (15 strain) in Hainan, all plant makes maternal (ripe fruit ear seed color; Black grain is 1: 1 with yellow grain fruit ear and separates); Zheng 58 makes male parent, backcrosses, and forms a selfing generation * Zheng 58 kind of backcrossing; Choose three black fruit ears of backcrossing after the maturation, dry threshing respectively and plant.
Summer in 2004; The seed of three black kind of fruit ears of backcrossing is respectively broadcast delegation's (19 strain) in our company experimental field; Whole plant selfings in flowering stage; Generation has the selfing generation fruit ear (black grain does not still have rule with the quantity of yellow grain fruit ear) of black grain and yellow grain, chooses three black grain selfing generation fruit ears from kind of the head progeny row of backcrossing, and dries threshing respectively and plants.
Winter in 2004; Three black grain selfing generation fruit ear seeds of sowing in Hainan, every tassel seed is broadcast delegation, and flowering stage, whole plant carried out the selfing second time; Generation has the selfing two generations fruit ear (black grain is 1: 1 with yellow grain fruit ear and separates) of black grain and yellow grain; Choose three black grain selfing two generations fruit ears, dry threshing and plant, supply next to continue selfing choosing system from generation to generation.According to the method described above, altogether through six separation screenings of selfing from generation to generation, breed the black grain of horse flute profile corn inbred line H83 forever in the summer in 2006.
D. the osculant black grain corn inbred line seed selection of H52 forever
Winter in 2002 was done maternal with black 231 in Hainan, the prosperous 7-2 of yellow corn inbred line that kind industry company of effluent south Jinboshi obtains makes male parent, is made into black 231 * prosperous 7-2 first-filial generation (F1).
Summer in 2003 is in black 231 * prosperous 7-2 first-filial generation (F1), 1 row district's 19 strains of our company's experimental field sowing; Whole selfings in flowering stage; Generate selfing generation fruit ear seed, the fruit ear seed color after the maturation has two kinds of fruit ears of black grain and yellow grain, but does not have rule; Choose the black grain of 3 selfing generation fruit ear, dry threshing respectively and plant.
2003 winters; The seed of the black grain of 3 selfing generation fruit ear is respectively broadcast delegation's (15 strain) in Hainan, all plant does maternal (the black grain of the fruit ear seed color after the maturation is 1: 1 with yellow grain fruit ear and separates), and prosperous 7-2 makes male parent; Backcross; Form a selfing generation * prosperous 7-2 kinds of backcrossing, choose three black grains fruit ears of backcrossing after the maturation, dry and distinguish threshing and plant.
Summer in 2004; Three black kind of fruit ear seeds of backcrossing are respectively broadcast delegation's (19 strain) in our company experimental field; Whole plant selfings in flowering stage; Generation has the selfing generation fruit ear (black grain does not still have rule with the quantity of yellow grain fruit ear) of black grain and yellow grain, chooses three black grain selfing generation fruit ears from kind of the head progeny row of backcrossing, and dries threshing respectively and plants.
Winter in 2004; The seed of three black grain selfing generation fruit ears of sowing in Hainan, the seed of every fringe is broadcast delegation, and flowering stage, whole plant carried out the selfing second time; Generation has the selfing two generations fruit ear (black grain is 1: 1 with yellow grain fruit ear and separates) of black grain and yellow grain; Choose three black grain selfing two generations fruit ears, dry threshing and plant, supply next to continue selfing choosing system from generation to generation.According to the method described above, altogether through six separation screenings of selfing from generation to generation, breed the black grain of horse flute profile corn inbred line H52 forever in the summer in 2006.
E. beautiful 208 the seed selection forever of black grain corn hybrid seed
Winter in 2006 was female parent in Hainan with H83 forever, was male parent with H52 forever; The temporary called after H211 of black grain corn hybrid seed is bred in hybridization, and the summer in 2007 was carried out the output comparative test in our unit, 598.2 kilograms of average yield per mus; Comparison was according to kind of Zheng Dan 958 raising the output 3.6%, and the economical character performance was better promoted Hebei province's corn variety preliminary experiment in 2008; Kind name H211 names and is forever beautiful 208; Pressed special corn through the authorization of the Hebei province variety certification committee in 2011 through Hebei province's corn regional test and production test in 2010, authorization is numbered the Ji and examines beautiful No. 2011005.
Of the present invention kind of method for planting characteristics are: suitable dense planting, and planting density 4000-4500 strain ∕ mu, field management needs the heavy dressing jointing fertile, takes out and need water sufficient water before male, and the fruit ear flavescence can be gathered in the crops, if results evenings, plants skin and has the be full of cracks Signs of Mould that absorbs water.

Claims (5)

1. deceive grain corn hybrid seed beautiful 208 breeding method forever for one kind, it is characterized in that: specifically may further comprise the steps:
A. the seed selection of the black grain of horse flute profile corn inbred line black 151
To deceive glutinous No. 3 is basic material, and with 478 hybridization of horse flute profile yellow corn inbred line, its first-filial generation (F1) is bred the black corn inbred line black 151 of horse flute profile through five separation screenings of selfing from generation to generation;
B. the seed selection of the black grain of osculant corn inbred line black 231
To deceive glutinous No. 3 is basic material, and with 5237 hybridization of solids type yellow corn inbred line, its first-filial generation (F1) is bred the black corn inbred line black 231 of osculant through five separation screenings of selfing from generation to generation;
C. the horse flute profile black grain corn inbred line seed selection of H83 forever
Is background material with the black grain of horse flute profile corn inbred line black 151 with horse flute profile yellow corn inbred Zheng 58, breeds the black grain of horse flute profile corn inbred line H83 forever;
D. the osculant black grain corn inbred line seed selection of H52 forever
Is background material with the black grain of osculant corn inbred line black 231 with the prosperous 7-2 of solids type yellow corn inbred line, breeds the black grain of osculant corn inbred line H52 forever;
E. beautiful 208 the seed selection forever of black grain corn hybrid seed
So that H83 inbred line work is maternal forever, the H52 inbred line is made male parent forever, and black grain corn hybrid seed jade 208 is forever bred in hybridization.
2. a kind of black grain corn hybrid seed according to claim 1 is beautiful 208 breeding method forever; It is characterized in that black glutinous No. 3 and horse flute profile yellow corn inbred line 478 hybridization back generation first-filial generations (F1) among the said step a; Select for use the black grain of the non-glutinous matter fruit ear seed among the F1 to do kind of a continuous selfing again; Wherein each self-generation all selects for use the black grain of the non-glutinous matter fruit ear seed of a generation to plant, and through five separation screenings of selfing from generation to generation, breeds the horse flute profile and deceives grain corn inbred line black 151.
3. a kind of black grain corn hybrid seed according to claim 1 is beautiful 208 breeding method forever; It is characterized in that black glutinous No. 3 and solids type yellow corn inbred line 5237 hybridization back generation first-filial generations (F1) among the said step b; Select for use the black grain of the non-glutinous matter fruit ear seed among the F1 to do kind of a continuous selfing again; Wherein each self-generation all selects for use the black grain of the non-glutinous matter fruit ear seed of a generation to plant, and through five separation screenings of selfing from generation to generation, breeds osculant and deceives grain corn inbred line black 231.
4. a kind of black grain corn hybrid seed according to claim 1 is beautiful 208 breeding method forever; It is characterized in that the black grain of horse flute profile corn inbred line black 151 generates first-filial generation (F1) with horse flute profile yellow corn inbred Zheng 58 hybridization backs among the said step c; Choosing black grain fruit ear seed in (F1), to do after kind of the selfing generation be that the parent backcrosses with Zheng 58 again; The black grain fruit ear seed choosing again in the kind of backcrossing is done kind of a continuous selfing; Wherein each self-generation all selects for use the black grain fruit ear seed of a generation to plant, and through six separation screenings of selfing from generation to generation, breeds the black corn inbred line of horse flute profile H83 forever.
5. a kind of black grain corn hybrid seed according to claim 1 is beautiful 208 breeding method forever; It is characterized in that the black grain of the osculant corn inbred line black 231 in the said steps d generates first-filial generation (F1) with the prosperous 7-2 hybridization of solids type yellow corn inbred line back; Choosing black grain fruit ear seed in (F1), to do after kind of the selfing generation be that the parent backcrosses with prosperous 7-2 again; The black grain fruit ear seed choosing again in the kind of backcrossing is done kind of a continuous selfing; Wherein each self-generation all selects for use the black grain fruit ear seed of a generation to plant, and through six separation screenings of selfing from generation to generation, breeds the black corn inbred line of osculant H52 forever.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103181322A (en) * 2013-04-19 2013-07-03 河北冀南种业有限公司 Breeding method of br-2 short-gene-type maize dwarf 268

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1367999A (en) * 2001-02-06 2002-09-11 刘绍东 Method for breeding hybridized corn
CN101554134A (en) * 2009-05-21 2009-10-14 西北农林科技大学 Method for breeding waxy corn crossbreed
CN101889537A (en) * 2010-07-05 2010-11-24 李荣河 Method for preparing high-yield hybrid waxy corn seeds

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1367999A (en) * 2001-02-06 2002-09-11 刘绍东 Method for breeding hybridized corn
CN101554134A (en) * 2009-05-21 2009-10-14 西北农林科技大学 Method for breeding waxy corn crossbreed
CN101889537A (en) * 2010-07-05 2010-11-24 李荣河 Method for preparing high-yield hybrid waxy corn seeds

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103181322A (en) * 2013-04-19 2013-07-03 河北冀南种业有限公司 Breeding method of br-2 short-gene-type maize dwarf 268
CN103181322B (en) * 2013-04-19 2015-09-23 河北冀南种业有限公司 The breeding method of the short list 268 of a kind of br-2 dwarf type genes corn

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