CN102577691B - System using extremely low frequency pulsed electrical field to treat seeds and treatment method - Google Patents
System using extremely low frequency pulsed electrical field to treat seeds and treatment method Download PDFInfo
- Publication number
- CN102577691B CN102577691B CN201110349729.6A CN201110349729A CN102577691B CN 102577691 B CN102577691 B CN 102577691B CN 201110349729 A CN201110349729 A CN 201110349729A CN 102577691 B CN102577691 B CN 102577691B
- Authority
- CN
- China
- Prior art keywords
- seed
- low frequencies
- electric field
- voltage pulse
- seeds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a system using an extremely low frequency pulsed electric field to treat seeds, which comprises an extremely low frequency high voltage pulse generator, wherein a copper plate is respectively connected with two poles of the extremely low frequency high voltage pulse generator, the two copper plates are placed horizontally and serve as electrodes, and organic glass which is used for placing seeds is arranged between the two copper plates. A method which adopts the system to treat seeds includes the following steps: firstly, cleaning and sterilizing the seeds, killing surface microorganisms, and immersing the seeds in water for 3 to 24 hours; and then placing seeds which absorb water and germinate on the organic glass, and starting the extremely low frequency high voltage pulse generator to treat the seeds. According to the system using the extremely low frequency pulsed electric field to treat seeds and the treatment method, no pretreatment of seeds in any mode is required, only the extremely low frequency pulsed electric field is used for facilitating germination and growth of seeds, environmental pollution is avoided, nucleuses are not affected, chromosome variation of cells and environment hazards are avoided, the system and the method are simple and practical, and the effects are obvious.
Description
Technical field
The invention belongs to the low-frequency pulse electric field technical field, relate to a kind of method that promotes Seed Germination and growth, be specifically related to a kind of system that adopts the very low frequencies impulse electric field to process seed, the invention still further relates to the method that adopts said system to process seed.
Background technology
Plant seed can improve resistance and the output of seed vitality, promotion seed germination and growth of seedling, increase seed after treatment.The method of existing seed treatment mainly contains chemical method and physical method, chemical method is used various chemical reagent, hormone or drug treating seed, as be CN1915032 at publication number, within open day, be: 2007.02.21, the patent of invention that patent name is " a kind of for promoting the seed treatment of seed germination ", utilize gibberellin, methyl α-naphthyl acetate, zeatin and carbendazim to promote seed germination.Due to the use meeting of chemical agent in chemical method to environment, physical method comes into one's own, that successively studied has electrostatic processes, ultrasonic processing method, nuclear radiation processing method, magnetized drinking water processing method and a low energy ion processing method etc., wherein, high-voltage electrostatic field research is maximum.As be CN1031168 at publication number, within open day, be: 1989.02.22, in the patent of invention that patent name is " apparatus for treating seeds by electrostatic field ", process seed with high-voltage electrostatic field.In order to improve action effect, some comprehensive processing methods have also appearred, as be CN1295785 at publication number, within open day, be: 2001.05.23, in the patent of invention that patent name is " method for the treatment of seed in ac electric field ", electrostatic field is processed with chemical treatment method and integrated, the nutrient solution pretreatment first formed with ABT root-inducing powder, gibberellin, PEG conditioning agent and other chemical reagent, and then use the electric field treatment seed; Be CN102144443 at publication number, within open day, be: 2011.08.10, patent name is in " a kind of method that seed germination is processed " patent of invention, with electrochemical method, promotes seed germination.But, in these methods, because electric field action mechanism is unclear, cause used electric field dosage very chaotic, effect is very little in actual applications, the result of contradiction even often occurs, is difficult to application; Use due to chemical reagent in comprehensive method works the mischief to environment.
Summary of the invention
The purpose of this invention is to provide a kind of system that adopts the very low frequencies impulse electric field to process seed, solve in the physical technique that has seed treatment now the unconspicuous problem of the facilitation effect of seed germination and growth of seedling.
Another object of the present invention is to provide a kind of method that adopts said system to process seed.
The technical solution adopted in the present invention is, adopt the very low frequencies impulse electric field to process the system of seed, comprise the very low frequencies high-voltage pulse generator, the two poles of the earth of very low frequencies high-voltage pulse generator are connected with respectively a copper coin, two parallel placements of copper coin, as electrode, are provided with for placing the lucite of seed between two copper coins.
Another technical scheme of the present invention is, adopt the very low frequencies impulse electric field to process the method for seed, application adopts the very low frequencies impulse electric field to process the system of seed, the structure of this system is: comprise the very low frequencies high-voltage pulse generator, the two poles of the earth of very low frequencies high-voltage pulse generator are connected with respectively a copper coin, two parallel placements of copper coin, as electrode, are provided with between two copper coins for placing the lucite of seed;
Specifically according to following steps, implement:
Step 1: seed is cleaned, sterilizes, kill surface microorganism, soak in water 3～24 hours;
Step 2: the seed after the water suction that step 1 is obtained is sprouted is placed on lucite, opens the very low frequencies high-voltage pulse generator to the seed effect.
Characteristics of the present invention also are, step 2 is wherein opened the very low frequencies high-voltage pulse generator to the seed effect, electric-field intensity 100KV/m～500KV/m is set, the direction of an electric field vertical ground is downward, pulse frequency is 0.1～20Hz, pulsewidth is 10ms～100ms, acts on 2～8 hours every day, effect 5-6 days.
The invention has the beneficial effects as follows, without seed being carried out to the pretreatment of any mode, utilize merely the very low frequencies impulse electric field to promote Germination and growth, environmentally safe, without impact, can not cause cell chromosome variation and environmental hazard on cell nucleus, simple and practical, successful.
The accompanying drawing explanation
Fig. 1 is that the present invention adopts the very low frequencies impulse electric field to process the structural representation of the system of seed;
Fig. 2 is the schematic diagram of very low frequencies high-voltage pulse generator in the present invention;
Fig. 3 is the very low frequencies high-voltage pulse adopted in the present invention;
Fig. 4 is the impact of very low frequencies impulse electric field on sprouting mung bean delayed luminescence integrated intensity in the embodiment of the present invention;
Fig. 5 is the impact of very low frequencies impulse electric field on the luminous integrated intensity of germinating corn in the embodiment of the present invention.
In figure, 1. very low frequencies high-voltage pulse generator, 2. copper coin, 3. lucite.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in detail.
The present invention adopts the very low frequencies impulse electric field to process the structure of the system of seed, as shown in Figure 1, by sample room and 1 two parts of very low frequencies high-voltage pulse generator, formed, the two poles of the earth of very low frequencies high-voltage pulse generator 1 are connected with respectively a copper coin 2, the copper coin 2 of two parallel placements, as electrode, is provided with the lucite 3 for placing seed between two copper coins 2.High-voltage pulse at the Electro-pulsing Field produced between the two poles of the earth on seed.
The programmable pulse string generator that in Fig. 1, the very low frequencies high-voltage pulse generator is designed by single-chip microcomputer and LC oscillation circuit and high-frequency pulse transformer form, and its theory structure block diagram as shown in Figure 2.High-frequency pulse string drives the LC loop to produce and coerces vibration, the transient process of vibration realizes with boosting inverter frequently, make the crest voltage of train of pulse rise to the left and right into about 1000V, after the secondary booster through high-frequency pulse transformer and the LPF that formed by the capacitance-resistance electronic device, obtain the very low frequencies high-voltage pulse.The exportable amplitude high pressure of this system in 6KV～20KV scope continuously adjustable, output frequency at the very low frequencies high-voltage pulse of 0.1Hz～15Hz, Fig. 3 for oscillograph at the timing chart of between Fig. 1 two-plate, through high pressure bar (impedance 50M Ω) and oscilloprobe, decaying after 4000 times and measuring.
The present invention adopts the very low frequencies impulse electric field to process the method for seed, specifically according to following steps, implements:
Step 1: seed is cleaned, sterilizes, kill surface microorganism.In water, soak 3～24 hours, with abundant imbibition.Seed of the present invention comprises the various plant seeds such as cereal, beans and greengrocery.
Step 2: the seed after water suction is sprouted is put into two parallel-plate electrodes and is stood Electro-pulsing Field, act on 2～8 hours every day, electric-field intensity 100KV/m～500KV/m, direction of an electric field vertical ground are downwards, pulse frequency is that 0.1～20Hz, pulsewidth are 10ms～100ms.Acting on the seed germination acceleration of processing through impulse electric field two days later, cellular metabolism, seedling fresh weight, root length and bud length etc. have been compared notable difference with the contrast without the extra pulse electric field treatment.
The method of the invention is with very low frequencies Electro-pulsing Field seed defined above, and the resonance effects fluctuateed by impulse electric field and cell membrane potential impacts structure and the function of epicyte, reaches the effect that promotes Germination and growth.Foundation of the present invention is: 1. " every directly leading friendship " due to the cell capacitance device acts on, seed cell film with double electrical layers electrostatic field to external world has shielding action, the extraneous static field is difficult to permeates cell membranes to the enzymic activity in cytoplasm and cellular metabolism generation effect, and the very low frequencies impulse electric field both can have been had an effect to epicyte, also can permeates cell membranes, the enzyme system generation effect in cell membrane; 2. the very low frequencies impulse electric field can not act on the cell inner membrance, on the not impact of the organelles such as intracellular cell nucleus, thereby can not cause cell damage; 3. the frequency of cell self potential fluctuation is at 0.1～20Hz, the very low frequencies Electro-pulsing Field that proportion is 0.1～20Hz left and right is in seed, cause the resonance of cell potential, thereby can cause remarkable impact to the ionic pump on epicyte and enzyme system, reach and promote the purpose of sprouting.
In the present invention, crucial parameter is electric-field intensity 100KV/m～500KV/m, pulse frequency 0.1～20Hz and pulsewidth 10ms～100ms, this is because the electric-field intensity of this strength range can cause disturbance to the structure of epicyte, the pulse frequency of this scope and cell membrane self potential fluctuation frequency is close, be easy to occur current potential resonance, ms level pulsewidth can not cause the damage of cell membrane.If operational factors surpasses this scope, or action effect is not obvious, or causes cellular damage, suppress Germination and growth.
Embodiment
By Zhengnong 7278 corn and No. 2 mung bean seed 0.2%HgCl of medium green
2sterilization 2min, fully imbibition in distilled water after washing.Select the seed of uniformity, evenly be placed in culture dish, a Medium speed filter paper is placed in the culture dish bottom, adds appropriate distilled water, is placed in (27 ± 0.2 ℃) constant incubator and cultivates in the dark, regularly adds distilled water every day.
The corn that shows money or valuables one carries unintentionally and mung bean seed are placed in two pole plates shown in Fig. 1 and process, and the field intensity direction is downward, processes 8h every day, processes continuously 5 days, process direction of an electric field downwards, electric-field intensity is that 100KV/m, pulse frequency are 1Hz, pulsewidth 80ms.Separately get identical seed as a control group, cultivate under the same conditions, except processing without impulse electric field, a tangent condition homogeneous phase is same.In experiment, processed group and control group are respectively established five repetitions.Measure the delayed luminescence integrated intensity of seed the every day in electric field treatment to obtain cellular metabolism information, measure respectively corn seedling control group and processed group when electric field treatment 5d in the long and bud of the root of 25 strain corn seedlings long with mung bean seedlings control group and processed group in the root of 30 strains long and bud is long.
The delayed luminescence integrated intensity that Fig. 4 is control group and processed group in the Mung Bean Germination process, as seen from Figure 4, when sprouting second day and the 3rd day, the delayed luminescence integrated intensity of control group and processed group there is no significant change, but the delayed luminescence integrated intensity of processed group is apparently higher than contrast when the 4th day and the 5th day.The variation that Fig. 5 is germinating corn delayed luminescence, as seen from Figure 5, along with the sprouting process, the delayed luminescence integrated intensity of the processed group of process Electro-pulsing Field, apparently higher than control group, presents very large difference.Because the size of delayed luminescence integrated intensity has represented the power of cellular metabolism, therefore, Fig. 4 and Fig. 5 show, impulse electric field is processed the cellular metabolism that has promoted germinating corn and mung bean seed.
Table 1 is the impact that in the embodiment of the present invention, the very low frequencies impulse electric field is long on mung bean sprouts and root is long; Table 2 is very low frequencies impulse electric field impacts on maize bud length, radical and root length in the embodiment of the present invention.In table 1, the long control group of processing without impulse electric field that all obviously is better than of the bud of the mung bean seed of processing through very low frequencies impulse electric field length and root, growth rate has reached respectively 65% and 80.2%; In table 2, the bud of corn processed group is long, long the comparing with contrast with radical of root all has significantly increase, and growth rate has reached respectively 94%, 83.3% and 196%.Statistical analysis shows, processed group is compared with control group, the difference of each index all reaches utmost point significance level (P<0.01), demonstrated electric-field intensity and be 100KV/m, pulse frequency and be the Germination and growth that very low frequencies impulse electric field that 1Hz, pulsewidth are 80ms has obviously promoted mung bean and corn.
The impact that table 1 very low frequencies impulse electric field is long on mung bean sprouts and root is long
Table 2 very low frequencies impulse electric field is on maize bud length, radical and the long impact of root
Claims (2)
1. adopt the very low frequencies impulse electric field to process the device of seed, it is characterized in that, comprise very low frequencies high-voltage pulse generator (1), the two poles of the earth of very low frequencies high-voltage pulse generator (1) are connected with respectively a copper coin (2), the parallel placement of two copper coins (2), as electrode, is provided with the lucite (3) for placing seed between two copper coins (2); The low pass filter that programmable pulse string generator, LC oscillation circuit, high-frequency pulse transformer and the capacitance-resistance electronic device that described very low frequencies high-voltage pulse generator (1) is designed by the single-chip microcomputer connected successively forms forms.
2. adopt the very low frequencies impulse electric field to process the method for seed, it is characterized in that, application adopts the very low frequencies impulse electric field to process the device of seed, the structure of this device is: comprise very low frequencies high-voltage pulse generator (1), the two poles of the earth of very low frequencies high-voltage pulse generator (1) are connected with respectively a copper coin (2), the parallel placement of two copper coins (2), as electrode, is provided with the lucite (3) for placing seed between two copper coins (2); The low pass filter that programmable pulse string generator, LC oscillation circuit, high-frequency pulse transformer and the capacitance-resistance electronic device that described very low frequencies high-voltage pulse generator (1) is designed by the single-chip microcomputer connected successively forms forms;
Specifically according to following steps, implement:
Step 1: seed is cleaned, sterilizes, kill surface microorganism, soak 3-24 hour in water;
Step 2: the seed after the water suction that step 1 is obtained is sprouted is placed on lucite (3), open very low frequencies high-voltage pulse generators (1) to the seed effect, it is 100KV/m that electric-field intensity is set, the direction of an electric field vertical ground is downward, pulse frequency is 1Hz, pulsewidth is 80ms, acts on 8 hours every day, acts on 5 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110349729.6A CN102577691B (en) | 2011-11-08 | 2011-11-08 | System using extremely low frequency pulsed electrical field to treat seeds and treatment method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110349729.6A CN102577691B (en) | 2011-11-08 | 2011-11-08 | System using extremely low frequency pulsed electrical field to treat seeds and treatment method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102577691A CN102577691A (en) | 2012-07-18 |
| CN102577691B true CN102577691B (en) | 2014-01-08 |
Family
ID=46467383
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110349729.6A Expired - Fee Related CN102577691B (en) | 2011-11-08 | 2011-11-08 | System using extremely low frequency pulsed electrical field to treat seeds and treatment method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102577691B (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107135705A (en) * | 2017-05-25 | 2017-09-08 | 上海交通大学 | Seed priming processing method based on HEHA |
| CN107182361B (en) * | 2017-06-06 | 2022-06-21 | 浙江大学 | Corn seed vitality on-line measuring device based on electrical impedance |
| CN107388348A (en) * | 2017-06-19 | 2017-11-24 | 西安理工大学 | A kind of green plant air purifier and the method for purifying air |
| CN107549000B (en) * | 2017-10-22 | 2022-11-08 | 王瑛 | Intelligent electrolysis air nutrition incubation seedling system and incubation method |
| TR201720295A2 (en) | 2017-12-13 | 2019-06-21 | Bolu Abant Izzet Baysal Ueniversitesi | Pulsed electric field system for processing granular products. |
| CN109121810A (en) * | 2018-10-25 | 2019-01-04 | 中国农业科学院农业环境与可持续发展研究所 | A kind of cultural method and device preventing fruits and vegetables calcium deficiency |
| CN110786100A (en) * | 2019-11-12 | 2020-02-14 | 哈尔滨盛世康虹生物技术有限公司 | Biological seed bopp apparatus |
| RU2734975C1 (en) * | 2019-11-21 | 2020-10-26 | Владимир Александрович Шиблев | Method of exposing cereal and fruit crops seeds to pre-sowing treatment with physical fields |
| CN112844846B (en) * | 2021-02-23 | 2022-09-16 | 上海丸西空环保科技有限公司 | Multi-frequency pulse discharge high-temperature flue gas dust collection device and dust collection method |
| CN114667821A (en) * | 2022-03-29 | 2022-06-28 | 内蒙古蒙草草种业有限公司 | Method for improving germination rate of torch tree seeds |
| CN116746323B (en) * | 2023-07-11 | 2024-05-28 | 济南市农业科学研究院 | Tomato seed treatment method and equipment |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2561194Y (en) * | 2002-03-06 | 2003-07-23 | 刘启武 | High-efficiency seed optimizing processor |
| RU2412574C2 (en) * | 2009-05-14 | 2011-02-27 | Общество с ограниченной ответственностью "Научно-производственное предприятие "Импульсные наукоемкие технологии"-ООО "НПП ИНТЕХ" | Method and device for pre-sowing stimulating and disinfecting treatment of seeds |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001136812A (en) * | 1999-11-17 | 2001-05-22 | Sanyo Electric Co Ltd | Apparatus for improving germination rate of seeds utilizing high voltage and method of improving germination rate of seeds using the same |
-
2011
- 2011-11-08 CN CN201110349729.6A patent/CN102577691B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2561194Y (en) * | 2002-03-06 | 2003-07-23 | 刘启武 | High-efficiency seed optimizing processor |
| RU2412574C2 (en) * | 2009-05-14 | 2011-02-27 | Общество с ограниченной ответственностью "Научно-производственное предприятие "Импульсные наукоемкие технологии"-ООО "НПП ИНТЕХ" | Method and device for pre-sowing stimulating and disinfecting treatment of seeds |
Non-Patent Citations (3)
| Title |
|---|
| Evelina Costanzo.The influence of an electric field on the growth of soy seedlings.《Journal of Electrostatics》.2008,第66卷(第7期), |
| JP特开2001-136812A 2001.05.22 |
| The influence of an electric field on the growth of soy seedlings;Evelina Costanzo;《Journal of Electrostatics》;20080521;第66卷(第7期);第417、418页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102577691A (en) | 2012-07-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102577691B (en) | System using extremely low frequency pulsed electrical field to treat seeds and treatment method | |
| CN102696472A (en) | Device and method for producing nuisanceless bean sprouts by adopting extremely-low-frequency pulsed electric field | |
| Takaki et al. | Improvements in plant growth rate using underwater discharge | |
| CN101686638B (en) | Seed treatment with PRF | |
| CN103891454B (en) | Method for increasing germination rate of soybean seeds | |
| CN102559483B (en) | Device and method for real-time monitoring of behaviors and states of cells | |
| CN104871845B (en) | It is a kind of to promote to transplant the method that crop nutrition content absorbs based on high-voltage electrostatic field | |
| CN110622648A (en) | Alfalfa seed treatment method and cold plasma test platform | |
| CN102598914A (en) | Method for breaking through dormancy of magnolia biloba seeds | |
| CN202949768U (en) | System for breading plant seedlings under compound induction of magnetic field and electric field | |
| CN105532121A (en) | Sterile sowing method for ginseng seeds | |
| CN106332577A (en) | Full-automatic plant seed yield-increase processing device | |
| CN111011191A (en) | Highland barley malt rich in melatonin and production method thereof | |
| CN207054041U (en) | A kind of seed magnetization device | |
| CN104087629A (en) | Method for increasing yield of intercellular polysaccharide in phellinus igniarius mycelium fermentation process by utilizing ultrasonic technique | |
| CN211152670U (en) | Electric field treatment device capable of improving crop seed yield and quality | |
| CN100356836C (en) | Multifunctional sonic wave processing technology and sonic wave generator for increasing quality plant production with protection | |
| CN109385370A (en) | A kind of quick endothelialization device and method thereof of intravascular stent | |
| CN107926679A (en) | A kind of leaf vegetables growth rate promote device based on high-pressure pulse electric | |
| Sonoda et al. | Growth control of leaf lettuce using pulsed electric field | |
| CN211960460U (en) | N-S quantum energy wave hydrogen plant seedling raising equipment | |
| Kadowaki et al. | Stimulation and Inhibition of Arabidopsis Seed Germination with Repetitive Barrier Discharges Produced by Polarity‐Reversed Voltage Pulses | |
| CN105039178A (en) | Phlebopus portentosus basidiospore germination method | |
| CN202744371U (en) | Electromagnetization generation device for water-saving drip irrigation of saline alkali soil | |
| CN112930920A (en) | Method for promoting growth of tissue culture seedlings of detoxified potatoes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140108 Termination date: 20201108 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |