CN102558386A - Method for extracting pectin from sweet potato dregs - Google Patents
Method for extracting pectin from sweet potato dregs Download PDFInfo
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- CN102558386A CN102558386A CN2012100151750A CN201210015175A CN102558386A CN 102558386 A CN102558386 A CN 102558386A CN 2012100151750 A CN2012100151750 A CN 2012100151750A CN 201210015175 A CN201210015175 A CN 201210015175A CN 102558386 A CN102558386 A CN 102558386A
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Abstract
The invention discloses a method for extracting pectin from sweet potato dregs. The key point of the technical scheme is that: the method comprises the following steps of: (1) weighing the sweet potato dregs, high-temperature alpha-amylase, glucuroide, and extractant for later use; (2) mixing the sweet potato dregs and water, adding the high-temperature alpha-amylase, and filling into a stirring and heating device; raising temperature, opening and stirring, heating, adding glucuroide, preserving heat, inspecting whether a mixture becomes blue by using an iodine solution, and if the mixture becomes blue, stopping heating and stirring; (3) filtering matter remained in the device, and washing by using water to obtain filter dregs, namely the sweet potato dregs from which starch is removed; (4) mixing the sweet potato dregs from which the starch is removed and water, adding extractant, stirring and preserving heat, heating and filtering, performing centrifugation on filtrate, allowing supernate to flow through a membrane separation device, and concentrating; (5) adding ethanol into a concentrated solution, fully stirring, centrifuging, and precipitating to obtain the pectin. The extraction method has low requirements on equipment, is high in practicability, has comprehensive economic benefit, and reduces environment pollution caused by the sweet potato dregs.
Description
Technical field:
The present invention relates to the production of pectin, particularly a kind of method of from sweet potato residue, extracting pectin.
Background technology:
Pectin is to receive FAO/WHO foodstuff additive joint committee to recommend not receive the natural nutrition health-care additive of addition restriction.Natural pectin is present in cell walls and the cell middle layer of all terrestrial plants, and commodity pectin extracts from orange peel and apple residue and obtains at present.
Find that through check and analysis except moisture, also contain remaining starch of 40-50% and 20% pectin in the sweet potato waste residue of suitability for industrialized production, sweet potato residue must be removed remaining starch earlier before extracting.Sweet potato residue is the sub product in the sweet potato course of processing, is difficult for storing, and major part is abandoned as refuse in the batch production production process; Not only causing environmental pollution, also is the significant wastage to affluent resources in the potato slag, has reduced utilization ratio and the sweet potato economic benefit of processing of sweet potato in the course of processing simultaneously; How effectively to utilize the pectin resource in the potato slag; Developing high value added product, improve utilization ratio and the sweet potato economic benefit of processing of sweet potato, is sweet potato processing industry problem demanding prompt solution.
Summary of the invention:
The purpose of this invention is to provide that a kind of cost is lower, the method for from sweet potato residue, extracting pectin of environmental protection and energy saving.
Technical scheme of the present invention is, a kind of method of from sweet potato residue, extracting pectin, and it is characterized in that: it may further comprise the steps: (1) takes by weighing a certain amount of dried sweet potato residue, high-temperature, glucuroide respectively, and extraction agent is subsequent use; (2) sweet potato residue and water are mixed, add high-temperature, packing into has in the agitating heater; Heating unit is heated up, open stirring, temperature remains on 90-95 ℃, insulation 10-15min; Treat that temperature reduces to 55-60 ℃, add glucuroide, insulation 50-70min checks constant indigo plant with iodine liquid, stops heated and stirred; (3) surplus materials in the heating unit is filtered with 60 mesh sieves, filter residue washing 4-5 time obtains filter residue and is the sweet potato residue except that destarching; (4) it is evenly mixed to remove sweet potato residue and the water of starch; Proportionally add extraction agent; Stir 85-95 ℃ of insulation certain hour down, heat filtering, filtrating is carried out 5000-6000r/min; Centrifugal 15-25min, it is original 1/5-1/4 that supernatant is concentrated into volume through membrane separation unit (retaining molecular weight 50kD); (5) add ethanol in the liquid concentrator, make that ethanol content reaches more than 60% in the mixed solution, fully stir 1.5-2.5h, 6000-7000 r/min, centrifugal 15-25min, deposition is the pectin that from sweet potato residue, extracts.Said extraction agent is: EDTA (YD 30), ammonium oxalate, potassium oxalate, sodium polyphosphate, sulfurous acid.Said sweet potato residue is the residue of sweet potato after extracting beta-amylase and starch.The add-on of said high-temperature is the dried sweet potato residue of 10-20U/g, and the add-on of glucuroide is the dried sweet potato residue of 20-30U/g.
The present invention has following beneficial effect:
(1) method that the present invention relates to extract is lower to the requirement of equipment, practical, is easy to industrialization production;
(2) present method extraction pectin belongs to high methoxyl, high Zhi Huadu pectin;
(3) the inventive method is extracted pectin extraction yield height, and purity is high;
(4) pectin soluble of the present invention is good, and viscosity is high, and product can directly be used as natural health additive or medicine auxiliary material;
(5) the inventive method can improve the overall economic efficiency of sweet potato processing, reduces the environmental pollution that the potato slag causes.
Embodiment:
Describe the embodiment of the invention in detail,
Embodiment one,
(1) get the thick sweet potato residue of 40g respectively, add 1600mL water, transfer pH=6.5, increase temperature the dried sweet potato residue of AMS 15U/g, 95 ℃ of insulation preliminary hydrolyzed starches of 10min also destroy polygalacturonase; Cooling, temperature is reduced to 60 ℃, transfers pH4.5, adds the dried sweet potato residue of glucuroide 25U/g, and insulation 60min checks constant indigo plant with iodine liquid, stops heating; Subsequent use;
(2) sweet potato residue of enzymolysis in (1) being crossed filters with 60 mesh sieves, and filter residue cleans 4 times with 4 times of clear water, removes sugar, 55-60 ℃ of dry for standby.
(3) get the sweet potato residue 20g in (2), add 800mL water, add ammonium oxalate and EDTA then, make ammonium oxalate content reach 5 ‰; EDTA content reaches 1.75 ‰, transfers 6.5,85 ~ 95 ℃ of insulations of pH value of solution 3.0h; Stir speed (S.S.) 50r/min uses the filter cloth filtered while hot, carries out 6000 r/min then; 20min is centrifugal, collects supernatant, and is subsequent use.
(4) with membrane separation unit (molecular weight cut-off is the filter membrane of 50kD) supernatant in (3) is carried out concentration, it is original 1/4 that extracting solution is concentrated into volume.
(5) liquid concentrator in (4) is added 95% ethanol, make that ethanol content fully stirs 2h greater than 60% (V/V) in the mixing solutions, the centrifugal 20min of 6000 r/min, collecting precipitation, pectin gets product after the drying.
This instance extracts sweet potato pectin each item index: yield 12.3 ± 0.7%, purity 83 ± 2.5%, viscosity 24 ± 1.5%, Zhi Huadu 65 ± 1.4%, degree of gelation 130 ± 2.5%, solubleness 99.8 ± 1.4%.
Embodiment two,
(1) get the thick sweet potato residue of 40g respectively, add 1600mL water, transfer pH=6.5, increase temperature the dried sweet potato residue of AMS 15U/g, 95 ℃ of insulation preliminary hydrolyzed starches of 10min also destroy polygalacturonase; Cooling, temperature is reduced to 60 ℃, transfers pH4.5, adds the dried sweet potato residue of glucuroide 25U/g, and insulation 60min checks constant indigo plant with iodine liquid, stops heating; Subsequent use.
(2) sweet potato residue of enzymolysis in (1) being crossed filters with 60 mesh sieves, and filter residue cleans 4 times with 4 times of clear water, removes sugar, dry for standby.
(3) get the sweet potato residue 20g in (2), add 800mL water, add potassium oxalate and EDTA then, make potassium oxalate content reach 5 ‰, EDTA content reaches 1.75 ‰, transfers pH value of solution 6.5,85-95 ℃ of insulation 3.0h; Stir speed (S.S.) 50r/min uses the filter cloth filtered while hot, carries out 6000 r/min then, and 20min is centrifugal, collects supernatant, and is subsequent use.
(4) with membrane separation unit (molecular weight cut-off is the filter membrane of 50kD) supernatant in (3) is carried out concentration, it is original 1/4 that extracting solution is concentrated into volume.
(5) liquid concentrator in (4) is added 95% ethanol, make that ethanol content fully stirs 2h greater than 60% (V/V) in the mixing solutions, the centrifugal 20min of 6000 r/min, collecting precipitation, pectin gets product after the drying.
This instance extracts sweet potato pectin each item index: yield 13.8 ± 0.6%, purity 75 ± 1.6%, viscosity 23 ± 1.5%, Zhi Huadu 64 ± 1.5%, degree of gelation 130 ± 2.5%, solubleness 99.2 ± 1.6%.
Embodiment three,
(1) get the thick sweet potato residue of 40g respectively, add 1600mL water, transfer pH=6.5, increase temperature the dried sweet potato residue of AMS 15U/g, 95 ℃ of insulation preliminary hydrolyzed starches of 10min also destroy polygalacturonase; Cooling, temperature is reduced to 60 ℃, transfers pH4.5, adds the dried sweet potato residue of glucuroide 25U/g, and insulation 60min checks constant indigo plant with iodine liquid, stops heating; Subsequent use.
(2) sweet potato residue of enzymolysis in (1) being crossed filters with 60 mesh sieves, and filter residue cleans 4 times with 4 times of clear water, removes sugar, dry for standby.
(3) get the sweet potato residue 20g in (2), add 800mL water, add sodium polyphosphate and EDTA then, make the polyphosphoric acid sodium content reach 5 ‰, EDTA content reaches 1.75 ‰, transfers pH value of solution 7.0,85-95 ℃ of insulation 4.0h; Stir speed (S.S.) 50r/min uses the filter cloth filtered while hot, carries out 6000 r/min then, and 20min is centrifugal, collects supernatant, and is subsequent use.
(4) with membrane separation unit (molecular weight cut-off is the filter membrane of 50kD) supernatant in (3) is carried out concentration, it is original 1/4 that extracting solution is concentrated into volume.
(5) liquid concentrator in (4) is added 95% ethanol, make that ethanol content fully stirs 2h greater than 60% (V/V) in the mixing solutions, the centrifugal 20min of 6000 r/min, collecting precipitation, pectin gets product after the drying.
This instance extracts sweet potato pectin each item index: yield 12.6 ± 0.6%, purity 81 ± 19%, viscosity 22 ± 1.5%, Zhi Huadu 63 ± 1.4%, degree of gelation 146 ± 2.4%, solubleness 99.8 ± 1.0%.
Embodiment four,
(1) get the thick sweet potato residue of 40g respectively, add 1600mL water, transfer pH=6.5, increase temperature the dried sweet potato residue of AMS 15U/g, 95 ℃ of insulation preliminary hydrolyzed starches of 10min also destroy polygalacturonase; Cooling, temperature is reduced to 60 ℃, transfers pH4.5, adds the dried sweet potato residue of glucuroide 25U/g, and insulation 60min checks constant indigo plant with iodine liquid, stops heating; Subsequent use.
(2) sweet potato residue of enzymolysis in (1) being crossed filters with 60 mesh sieves, and filter residue cleans 4 times with 4 times of clear water, removes sugar, dry for standby.
(3) get the sweet potato residue 20g in (2), add 800mL water, add sulfurous acid solution solution, pH2.0,85-95 ℃ of insulation 2.0h, stir speed (S.S.) 50r/min; Use the filter cloth filtered while hot, carry out 6000 r/min then, 20min is centrifugal, collects supernatant, and is subsequent use.
(4) with membrane separation unit (molecular weight cut-off is the filter membrane of 50kD) supernatant in (3) is carried out concentration, it is original 1/4 that extracting solution is concentrated into volume.
(5) liquid concentrator in (4) is added 95% ethanol, make that ethanol content fully stirs 2h greater than 60% (V/V) in the mixing solutions, the centrifugal 20min of 6000 r/min, collecting precipitation, pectin gets product after the drying.
This instance extracts sweet potato pectin each item index: yield 11.0 ± 0.6%, purity 75 ± 2.8%, viscosity 24 ± 1.4%, Zhi Huadu 70 ± 1.4%, degree of gelation 140 ± 5%, solubleness 98.4 ± 2.3%.
The method that the present invention relates to extract is lower to the requirement of equipment, and is practical, is easy to industrialization production; It is high that method of the present invention is extracted the pectin extraction yield, and purity is higher; The pectin soluble that the present invention extracts is good, and viscosity is high, and product can directly be used as natural health additive or medicine auxiliary material; The inventive method can improve the overall economic efficiency of sweet potato processing, reduces the environmental pollution that the potato slag causes, friendly environment.
Claims (4)
1. method of from sweet potato residue, extracting pectin, it is characterized in that: it may further comprise the steps: (1) takes by weighing a certain amount of dried sweet potato residue, high-temperature, glucuroide respectively, and extraction agent is subsequent use; (2) sweet potato residue and water are mixed, add high-temperature, packing into has in the agitating heater; Heating unit is heated up, open stirring, temperature remains on 90-95 ℃, insulation 10-15min; Treat that temperature reduces to 55-60 ℃, add glucuroide, insulation 50-70min checks constant indigo plant with iodine liquid, stops heated and stirred; (3) surplus materials in the heating unit is filtered with 60 mesh sieves, filter residue washing 4-5 time obtains filter residue and is the sweet potato residue except that destarching; (4) it is evenly mixed to remove sweet potato residue and the water of starch; Proportionally add extraction agent; Stir 85-95 ℃ of insulation certain hour down, heat filtering, filtrating is carried out 5000-6000r/min; Centrifugal 15-25min, it is original 1/5-1/4 that supernatant is concentrated into volume through membrane separation unit (retaining molecular weight 50kD); (5) add ethanol in the liquid concentrator, make that ethanol content reaches more than 60% in the mixed solution, fully stir 1.5-2.5h, 6000-7000 r/min, centrifugal 15-25min, deposition is the pectin that from sweet potato residue, extracts.
2. a kind of method of from sweet potato residue, extracting pectin as claimed in claim 1, it is characterized in that: said extraction agent is: EDTA (YD 30), ammonium oxalate, potassium oxalate, sodium polyphosphate, sulfurous acid.
3. a kind of method of from sweet potato residue, extracting pectin as claimed in claim 1 is characterized in that: said sweet potato residue is the residue of sweet potato after extracting beta-amylase and starch.
4. a kind of method of from sweet potato residue, extracting pectin as claimed in claim 1, it is characterized in that: the add-on of said high-temperature is the dried sweet potato residue of 10-20U/g, the add-on of glucuroide is the dried sweet potato residue of 20-30U/g.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106243242A (en) * | 2016-08-31 | 2016-12-21 | 山西天骄食业有限公司 | A kind of utilize degraded the solubilization technique method that Fructus Jujubae pectin is prepared in extraction from Fructus Jujubae Fructus Jujubae slag |
| CN106361798A (en) * | 2016-08-18 | 2017-02-01 | 广东药科大学 | Extraction method of resin glycoside-type compound |
| CN106519067A (en) * | 2016-11-19 | 2017-03-22 | 朱莉 | Comprehensive utilization method for sweet potato residues |
| CN107095317A (en) * | 2017-06-28 | 2017-08-29 | 山东省食品发酵工业研究设计院 | A kind of method that soluble dietary fiber is extracted from sweet potato dregs |
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| CN101041699A (en) * | 2006-03-22 | 2007-09-26 | 中国农业科学院农产品加工研究所 | Sweet potato pectin and production technique thereof |
| CN101627825A (en) * | 2008-07-15 | 2010-01-20 | 中国农业科学院农产品加工研究所 | Novel method for extracting pectin from sweet potato dregs |
| CN101891840A (en) * | 2010-08-09 | 2010-11-24 | 黑龙江农垦北大荒马铃薯产业有限公司 | Method for extracting pectin by utilizing potato scraps |
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2012
- 2012-01-18 CN CN2012100151750A patent/CN102558386A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101041699A (en) * | 2006-03-22 | 2007-09-26 | 中国农业科学院农产品加工研究所 | Sweet potato pectin and production technique thereof |
| CN101627825A (en) * | 2008-07-15 | 2010-01-20 | 中国农业科学院农产品加工研究所 | Novel method for extracting pectin from sweet potato dregs |
| CN101891840A (en) * | 2010-08-09 | 2010-11-24 | 黑龙江农垦北大荒马铃薯产业有限公司 | Method for extracting pectin by utilizing potato scraps |
Non-Patent Citations (1)
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| 魏海香等: "果胶制备的研究进展", 《食品研究与开发》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106361798A (en) * | 2016-08-18 | 2017-02-01 | 广东药科大学 | Extraction method of resin glycoside-type compound |
| CN106361798B (en) * | 2016-08-18 | 2019-04-26 | 广东药科大学 | A kind of extracting method of Resin glycoside class compound |
| CN106243242A (en) * | 2016-08-31 | 2016-12-21 | 山西天骄食业有限公司 | A kind of utilize degraded the solubilization technique method that Fructus Jujubae pectin is prepared in extraction from Fructus Jujubae Fructus Jujubae slag |
| CN106519067A (en) * | 2016-11-19 | 2017-03-22 | 朱莉 | Comprehensive utilization method for sweet potato residues |
| CN107095317A (en) * | 2017-06-28 | 2017-08-29 | 山东省食品发酵工业研究设计院 | A kind of method that soluble dietary fiber is extracted from sweet potato dregs |
| CN107095317B (en) * | 2017-06-28 | 2020-09-18 | 山东省食品发酵工业研究设计院 | Method for extracting soluble dietary fiber from sweet potato residue |
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Application publication date: 20120711 |