CN102557797B - Preparation method of compound microbial calcium agent - Google Patents
Preparation method of compound microbial calcium agent Download PDFInfo
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- CN102557797B CN102557797B CN 201110456383 CN201110456383A CN102557797B CN 102557797 B CN102557797 B CN 102557797B CN 201110456383 CN201110456383 CN 201110456383 CN 201110456383 A CN201110456383 A CN 201110456383A CN 102557797 B CN102557797 B CN 102557797B
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- calcium sulfate
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- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 title claims abstract description 29
- 239000011575 calcium Substances 0.000 title claims abstract description 29
- 229910052791 calcium Inorganic materials 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 12
- 150000001875 compounds Chemical class 0.000 title abstract description 6
- 230000000813 microbial effect Effects 0.000 title abstract description 6
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 claims abstract description 26
- 244000005700 microbiome Species 0.000 claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 235000012907 honey Nutrition 0.000 claims abstract description 10
- 239000000725 suspension Substances 0.000 claims abstract description 8
- 239000008367 deionised water Substances 0.000 claims abstract description 6
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims abstract description 5
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 2
- 239000000047 product Substances 0.000 claims description 2
- 238000005303 weighing Methods 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 abstract description 14
- 235000007688 Lycopersicon esculentum Nutrition 0.000 abstract description 13
- 240000003768 Solanum lycopersicum Species 0.000 abstract description 13
- 235000013399 edible fruits Nutrition 0.000 abstract description 13
- 238000000034 method Methods 0.000 abstract description 11
- 239000007788 liquid Substances 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000000855 fermentation Methods 0.000 abstract description 3
- 238000013329 compounding Methods 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 239000011550 stock solution Substances 0.000 abstract 1
- 206010006956 Calcium deficiency Diseases 0.000 description 14
- 239000002689 soil Substances 0.000 description 12
- 230000000694 effects Effects 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 239000007921 spray Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- 230000000536 complexating effect Effects 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 235000014655 lactic acid Nutrition 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 238000009335 monocropping Methods 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 238000002161 passivation Methods 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 230000000243 photosynthetic effect Effects 0.000 description 2
- 230000003716 rejuvenation Effects 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 235000012976 tarts Nutrition 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- -1 add said preparation Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003124 biologic agent Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 235000003715 nutritional status Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000037039 plant physiology Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 239000002688 soil aggregate Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241001446247 uncultured actinomycete Species 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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- Fertilizers (AREA)
Abstract
The invention belongs to the technical field of multifunctional biological preparations, and provides a method for preparing a compound microbial calcium agent. The method comprises the following preparation step of: adding a 10 percent calcium sulfate suspension in a process for preparing an active liquid of compound microorganisms, and comprises the following specific steps of: compounding 3-5 percent of an EM stock solution, 2-5 percent of honey, 5-10 percent of a calcium sulfate suspension and 80-92 percent of deionized water according to volume proportions, and mixing uniformly; putting into a rotary shaking table of which the speed is 120-180 revolutions per minute under a room temperature enclosed condition for performing anaerobic fermentation for 3-5 days; and standing and filtering for separating solid from liquid to obtain a liquid preparation of compound microbial calcium. The method has the advantages that: the fermented calcium is in a chelated (complexed) state or a cation-anion balance state, is dissolved into water, and is easy to be absorbed by plants through foliar application, and physiological disorder (such as tomato fruit blossom-end rot caused by the lack of calcium) caused by the lack of calcium in plants can be eliminated effectively.
Description
Technical field
The invention belongs to the technical field of multifunctional biological agent, utilize the compound microorganism ferments technology that the calcium sulfate that is insoluble in water is transformed into water-soluble Calcium of intensifiens.Made complex microorganism calcium agent is used for foliage-spray can effectively solve the physiological barrier that the plant calcium deficiency causes (the tomato fruit blossom-end rot that causes as calcium deficiency).This microbial preparation cultivation manufacture craft is easy, the invention cost is low, using method is simple, has broad application prospects.
Background technology
Calcium is the essential nutritive element of plant, and plant is because the physiological disease that calcium deficiency causes is a lot, and the modal tomato fruit blossom-end rot that causes as calcium deficiency influences very big to yield and quality.Simultaneously at present in the facility plastic greenhouse agriculture production because multiple crop index is high and use chemical fertilizer in a large number, secondary salinization and the acidifying of soil are commonplace, make calcium passivation in soil become disappearance.The shortcoming of used calcium preparation is to be insoluble in water or to be easy to precipitation or passivation in environment in the agriculture production at present, is difficult for by plant absorbing; Though chelating or complexing calcium preparation are water-soluble, price is very high, is difficult to large scale application in agriculture production.
EM is the english abbreviation of Effective Microorganisms, and the Chinese meaning is effective microorganism, claims complex microorganism again, and it is good for professor's husband invention to dredge ball university ratio by Japan.EM probiotic bacterium stoste is that kind of microorganism was formed surplus photosynthetic bacterium, lactobacillus, yeast flora, actinomycete group, thread flora etc. belonged to 80 for 5 kind 10.The EM technology is a present biotechnology of range of application maximum in the world.Compare with general biotechnological formulation, it has complex structure, stable performance, multiple functional advantage, and up to now, EM is in more than 90 countries and regions widespread uses such as Japan, the U.S., Brazil, France, Taiwan.According to document announcement, EM is applicable to multiple fields such as plant husbandry, aquaculture, environmental protection, body-care.
At present domestic technical elements at alleviation hunger sign in plant shape has been obtained some patents of invention, but utilizes the patent of invention of the relevant active bio calcium preparation of microbial fermentation technology preparation few.
Summary of the invention
Goal of the invention:The present invention is directed to the plant calcium deficiency and cause physiological barrier, utilize microbial fermentation technology that the calcium sulfate that is insoluble in water is transformed into water-soluble Calcium of intensifiens.Said preparation science in industrialized agriculture is used, and can reach to improve the soil, effectively control the plant physiology obstacle that calcium deficiency causes and improve crop yield and improve the effect of quality.
Technical scheme:
The agent of a kind of complex microorganism calcium, it is characterized in that being obtained by following steps: per-cent meter by volume, 3%～5%EM stoste, 2%～5% honey, 5%～10% calcium sulfate suspension, 80%～92% deionized water mix, under the room temperature air tight condition, vibrate, anaerobically fermenting 3-5d leaves standstill after-filtration and gets liquid portion and concentrate and to form.
The present invention relates to the preparation method of a kind of complex microorganism calcium agent, it is characterized in that being obtained by following steps: per-cent meter by volume, 3%～5% volume percent EM stoste, 2%～5% honey, 5%～10% 10% calcium sulfate suspension, 80%～92% deionized water are mixed, under the room temperature air tight condition, the speed of putting into is the rotary shaking table anaerobically fermenting 3-5d of 120r/min～180r/min, leave standstill filtration, concentrated filtrate forms.
Described 10% calcium sulfate suspension is to take by weighing 100 gram calcium sulfate in 100 ml beakers, adds deionized water and heating for dissolving, is settled at last in 1000 milliliters of volumetric flasks.
Described honey is the honey product of purchasing in the supermarket, and its effect is as energy substance in microorganism rejuvenation, fermenting process.
Described complex microorganism is that EM stoste contains effective active microorganisms such as milk-acid bacteria, yeast, photosynthetic bacteria, and viable count is no less than 100,000,000/milliliter, and pH is not less than 3.8, and color is tawny, translucent liquid, and smell is denseer pure tart flavour or tart flavour.But its effect is meta-bolites such as organic acid chelating or the complexing calcium such as glucose, lactic acid that produce, and insoluble calcium is changed into solubility calcium.EM produces for liking peaceful happy environment friendly biological technology (Nanjing) company limited.
Technique effect:
A kind of preparation method at the complex microorganism calcium agent of eliminating the physiological disease that the plant calcium deficiency causes, it is cultivated, and manufacture craft is easy, application cost is low, using method is simple, range of application is wider.Can eliminate the calcium deficiency symptom of plant rapidly as foliage spray, can effectively prevent the tomato fruit blossom-end rot that is caused by calcium deficiency, improve the sugar degree of fruit, increase output.In the process of pouring water, add said preparation, can improve soil physical and chemical property, increase the retaining water retention capacity of soil, improve plant to the utilization ratio of nitrogen (N), phosphorus (P), potassium (K) inorganic nutrients, promote plant-growth.
⑴ the single soil nutrient that can cause to fertilize of the medium-term and long-term continuous cropping of tomato facility cultivation is lacked of proper care, and causes calcium deficiency physiological disease (tomato fruit blossom-end rot), and the yield and quality of tomato is brought disadvantageous effect.Test shows when finding that tomato has the calcium deficiency symptom, can eliminate the tomato fruit blossom-end rot that is caused by calcium deficiency in the complex microorganism calcium agent of foliage spray 5%, improves the sugar degree of fruit, increases output.
⑵ said preparation is used for irritating root as a kind of biotechnological formulation, can improve the chemical property of continuous cropping soil, improves soil nutrient status.In the process of pouring water, add said preparation, soil pH value is risen, increase the content of alkali-hydrolyzable nitrogen and available potassium, increase the retaining water retention capacity of soil, improve plant to the utilization ratio of nitrogen (N), phosphorus (P), potassium (K) inorganic nutrients, promote the beneficial microorganism growth and suppress pathogenic bacteria to the infecting of plant, promote soil aggregate to form.
⑶ bear fruit the phase at tomato, and the complex microorganism calcium agent of foliage-spray 3%-5% 2-3 time can effectively prevent the tomato fruit blossom-end rot that caused by calcium deficiency, improves the sugar degree of fruit, increases output.
Embodiment
Below in conjunction with 6 examples the present invention is carried out detailed narration, but the present invention is not limited to given embodiment.
Among the embodiment, earlier EM stoste, honey and water are mixed, and then add 10% calcium sulfate suspension in mixed solution, the speed of putting in confined conditions is the rotary shaking table anaerobically fermenting 3-5d of 120r/min～180r/min, leave standstill filtration, concentrated filtrate forms.Through the NY/T789-2004(composite microbiological fertilizer) method that provides of standard measures, and the concentrated solution pH 6.0-7.5 of the complex microorganism calcium preparation that each example is prepared, living bacteria count (CFU) are all greater than 1.0 hundred million/milliliter, Calcium of intensifiens content 0.5%-1.0%.
In the compound microorganism ferments process, the effect of honey is to provide C source and the energy for microorganism rejuvenation and metabolism, after microorganism obtains C source and the energy, become active state from dormant state, contain lot of organic acids (lactic acid, glucose), amino acid and many active substances in the complex microorganism liquid anaerobically fermenting meta-bolites, with calcium sulfate complexing or chelating, become dissolved state.Preparation, concentrated complex microorganism calcium preparation are further diluted the back irritate root as foliage spray or soil, in the plastic greenhouse of the plantation tomato of Nanjing vegetable or flower institute, use, obtained the tomato fruit blossom-end rot that effective prevention is caused by calcium deficiency, improve the sugar degree of fruit, effect such as increase output and improve the soil.
Claims (3)
1. the preparation method of complex microorganism calcium agent, it is characterized in that being obtained by following steps: per-cent meter by volume, 3%～5% volume percent EM stoste, 2%～5% honey, 5%～10% 10% calcium sulfate suspension, 80%～92% deionized water are mixed, under the room temperature air tight condition, the speed of putting into is the rotary shaking table anaerobically fermenting 3-5d of 120r/min～180r/min, leave standstill filtration, concentrated filtrate forms.
2. the preparation method of complex microorganism calcium according to claim 1 agent, it is characterized in that described 10% calcium sulfate suspension, be to take by weighing 100 gram calcium sulfate in 100 ml beakers, add deionized water and heating for dissolving, be settled at last in 1000 milliliters of volumetric flasks.
3. the preparation method of complex microorganism calcium according to claim 1 agent is characterized in that described honey is the honey product of purchasing in the supermarket.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110456383 CN102557797B (en) | 2011-12-31 | 2011-12-31 | Preparation method of compound microbial calcium agent |
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| CN 201110456383 CN102557797B (en) | 2011-12-31 | 2011-12-31 | Preparation method of compound microbial calcium agent |
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| CN102557797A CN102557797A (en) | 2012-07-11 |
| CN102557797B true CN102557797B (en) | 2013-09-25 |
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Families Citing this family (9)
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| CN102836454A (en) * | 2012-08-06 | 2012-12-26 | 河海大学 | Compound biological deodorant solution used in refuse landfills and refuse transfer stations |
| CN105028992B (en) * | 2015-06-15 | 2018-10-23 | 河海大学 | A kind of biological active calcium and its preparation method and application |
| CN105294344A (en) * | 2015-11-13 | 2016-02-03 | 山东郁茏生物科技有限公司 | Composite microbial fertilizer for promoting calcareous absorption of plants and preparation method thereof |
| CN105860981B (en) * | 2016-05-05 | 2019-03-22 | 河海大学 | A kind of facility acidified soil conditioner and preparation method thereof |
| CN106083297A (en) * | 2016-06-02 | 2016-11-09 | 河海大学 | A kind of manufacture method of training algae agent used for aquiculture |
| CN109503251A (en) * | 2018-10-25 | 2019-03-22 | 武汉大学 | A kind of preparation method and applications of biostimulant |
| CN110002912A (en) * | 2019-04-03 | 2019-07-12 | 李凤兰 | The rich calcium preparation of biofermentation and its application |
| CN111393237A (en) * | 2020-03-17 | 2020-07-10 | 河海大学 | Biological seaweed calcium fertilizer for facility tomato planting, and preparation method and application method thereof |
| CN112716994A (en) * | 2020-12-26 | 2021-04-30 | 付军磊 | Preparation method of active calcium |
Citations (4)
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|---|---|---|---|---|
| CN1322697A (en) * | 2000-05-10 | 2001-11-21 | 山东龙凤制造有限公司 | Preparation of multiple-biological organic and inorganic fertilizer |
| CN101591206A (en) * | 2009-05-27 | 2009-12-02 | 江西百叶神生态科技有限公司 | A kind of biological liquid fertilizer and preparation method thereof |
| CN101613225A (en) * | 2009-07-22 | 2009-12-30 | 山东光大肥业科技有限公司 | A kind of special apple organic-inorganic compound fertilizer that contains Calcium of intensifiens, iron nutrition and preparation method thereof |
| CN101805223A (en) * | 2010-03-16 | 2010-08-18 | 范鸿基 | High-efficiency biological soluble fertilizer and strain preparation thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3993907B2 (en) * | 1996-12-02 | 2007-10-17 | キリンフードテック株式会社 | Calcium absorption promoter |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1322697A (en) * | 2000-05-10 | 2001-11-21 | 山东龙凤制造有限公司 | Preparation of multiple-biological organic and inorganic fertilizer |
| CN101591206A (en) * | 2009-05-27 | 2009-12-02 | 江西百叶神生态科技有限公司 | A kind of biological liquid fertilizer and preparation method thereof |
| CN101613225A (en) * | 2009-07-22 | 2009-12-30 | 山东光大肥业科技有限公司 | A kind of special apple organic-inorganic compound fertilizer that contains Calcium of intensifiens, iron nutrition and preparation method thereof |
| CN101805223A (en) * | 2010-03-16 | 2010-08-18 | 范鸿基 | High-efficiency biological soluble fertilizer and strain preparation thereof |
Non-Patent Citations (2)
| Title |
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| JP特开平10-158173A 1998.06.16 |
| 张烈，等.有效微生物群特点及其在种植业中的应用.《生态农业研究》.2000,第8卷(第3期),第28-31页. * |
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Effective date of registration: 20171221 Address after: 210000 software Avenue, Yuhuatai District, Nanjing, Jiangsu Province, No. 11 Patentee after: Jiangsu Yimu Biotechnology Co.,Ltd. Address before: Xikang Road, Gulou District of Nanjing city of Jiangsu Province, No. 1 210098 Patentee before: HOHAI University |
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