CN102524883B - Sea cucumber polysaccharide health-care beverage and preparation method thereof - Google Patents
Sea cucumber polysaccharide health-care beverage and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a sea cucumber polysaccharide health-care beverage and a preparation method thereof. Fresh sea cucumbers are selected to serve as raw materials, and enzymolysis, enzyme deactivation, concentration, ethanol precipitation and drying are performed by controlling enzymolysis conditions after homogenate to prepare sea cucumber polysaccharide for standby application; hawthorn 40-80%, cassia seeds 10-30% and honeysuckle 10-30% are utilized to prepare natural plant extracting solution by adopting a conventional traditional Chinese liquid medicine extracting method; xylitol, erythritol and fructo-oligosaccharide are combined to prepare sweetener according to the ratio of 1:1:3; weighing the sea cucumber polysaccharide 1-10%, the natural plant extracting solution 5-15%, the sweetener 5-15% and the balance water by weight to be mixed, filled and sterilized to obtain the sea cucumber polysaccharide health-care beverage. The sea cucumber polysaccharide health-care beverage is rich in nutrition, moderate in sour and sweet and natural in special flavor and has health-care functions of reducing blood pressure and blood lipid, improving liver function, adjusting intestinal flora, enhancing immunity and the like. The preparation method is advanced in process, reasonable in working procedures and strong in operability.
Description
Technical field
The present invention relates to a kind of non-alcoholic beverage and processing thereof, especially a kind of sea cucumber polysaccharide health drink and preparation method thereof.
Background technology
In recent years, the increase of the decline of global environmental quality and crowd's life stress, make the crowd who is in sub-health state day by day increase, and simultaneously along with the enhancing with health perception of improving constantly of living standard, people select healthy food, drink gradually, and the generation of this demand makes drinks begin to be subjected to people's favor.Drinks can be by adjusting composition and the content ratio of natural nutrient in the beverage, to adapt to the needs of some special population nutrition.At present, on the market fast-selling drinks to comprise pulsing be the nutrients beverage of representative, with strength is run, dimension body etc. is representative sports drink with the energy drink of red Niu Wei representative etc.
Sea cucumber polysaccharide is the important component of sea cucumber body wall, and its content can account for more than 6% of trepang total organic matter.Sea cucumber body wall polysaccharide mainly contains two kinds, and a kind of is glycosaminoglycan extracted from sea cucumber or mucopolysaccharide, and by branch's heteroglycan that D-N-acetylamino galactosamine, D-glucuronic acid and L-fucose are formed, relative molecular mass is 4 to 50,000; Another kind is the sea cucumber fucoidin, is the straight-chain polysaccharide that is made of the L-fucose, and relative molecular mass is 8 to 100,000.Though both composition glycosyls are different, and part of hydroxyl generation Sulfation is arranged on their sugar chains, and sulfuric ester base class polysaccharide mass fraction is all about 32%.Now definite, the structure of 2 kinds of sea cucumber polysaccharides is special, and is all peculiar by sea cucumber.In recent years, both at home and abroad to sea cucumber polysaccharide, particularly the pharmacological action of sea cucumber mucopolysaccharide and isomeric compound thereof is studied, confirmed that it has multiple drug effect: 1. strengthen immunity; 2. suppress tumour; 3. promote fibrinolysis; 4. reduce blood viscosity; 5. anticoagulation; 6. neuroprotective tissue.
At present, yet there are no open report and the sale of the non-alcohol type drinks that includes the sea cucumber polysaccharide composition.
Summary of the invention
In order to overcome deficiency of the prior art, the invention provides a kind of sea cucumber polysaccharide health drink and preparation method thereof.This beverage not only nutritious, sour-sweet moderate, local flavor is natural, and bioavilability height, and have health care.Its preparation method technology advanced person, operation feasible.
The technical solution adopted for the present invention to solve the technical problems is: a kind of sea cucumber polysaccharide health drink, it is characterized in that: it comprises sea cucumber polysaccharide, natural botanical extraction liquid, sweetener and water, the percentage by weight of its each component is respectively: sea cucumber polysaccharide 1～10%, natural botanical extraction liquid 5～15%, sweetener 5～15%, surplus are water; Wherein, described sea cucumber polysaccharide is control sea cucumber enzymolysis, alcohol precipitation gained; Described natural botanical extraction liquid is to adopt conventional traditional Chinese medicine liquid extraction method to obtain by hawthorn, cassia seed, honeysuckle.
Described sweetener is to be combined according to 1: 1: 3 ratio of weight ratio by xylitol, antierythrite, FOS.
The conventional traditional Chinese medicine liquid extraction method of described employing obtains hawthorn, cassia seed, the honeysuckle of natural botanical extraction liquid, and the percentage by weight during its compatibility is: hawthorn 40～80%, cassia seed 10～30%, honeysuckle 10～30%.
A kind of sea cucumber polysaccharide preparation method of health-care beverage is characterized in that: through following technology
The preparation of a, sea cucumber polysaccharide
It is raw material that fresh sea cucumber is chosen in the processing of a1, raw material, sends into after cleaning and carries out fragmentation, homogenized in the tissue mashing machine;
A2, enzymolysis dilute the water that the sea cucumber milk of step a1 gained adds 3～10 times of its weight, regulating the pH value is 6.0～8.0, add trypsase, wherein tryptic consumption is that every gram sea cucumber is added 2000～4000 enzymes unit alive, the control temperature is 50～60 ℃, enzymolysis 2～4h gets sea cucumber hydrolysate; With this sea cucumber hydrolysate boiling water bath, enzyme 10～20min goes out;
A3, alcohol precipitation go out step a2 gained, and to be evaporated to relative density for 50～60 ℃ be 1～1.5 for sea cucumber hydrolysate behind the enzyme, and the cooling back is 95% ethanol with the concentration of 2～3 times of amounts, and alcohol precipitation left standstill 24～36 hours; The control rotating speed is 2000～4000r/min, centrifugal 10～15min, and collecting precipitation, 40～80 ℃ of dry processing of temperature control get sea cucumber polysaccharide, and are standby;
The preparation of b, natural botanical extraction liquid takes by weighing hawthorn 40～80%, cassia seed 10～30%, honeysuckle 10～30% by weight percentage, cleans, and adopts the steam water cooking method to process, and makes natural botanical extraction liquid more after filtration, and is standby;
The choose xylitol, antierythrite, FOS of c, sweetener are combined into sweetener, and be standby; It makes up by weight: xylitol: antierythrite: FOS=1: 1: 3;
The sea cucumber polysaccharide of d, allotment weighing step a preparation, the natural botanical extraction liquid of step b preparation, sweetener and the water that step c chooses mix, the percentage by weight of each component is during weighing: sea cucumber polysaccharide 1～10%, natural botanical extraction liquid 5～15%, sweetener 5～15%, surplus are water;
E, can, sterilization are carried out can with the mixed liquor of steps d gained, carry out sterilization processing, its sterilization mode again: intensification 10min, 105～115 ℃ of sterilization 15～40min, cooling 10min; Then it is cooled to central temperature less than 40 ℃, gets product.
The present invention is as raw material with fresh sea cucumber, adopt modern biotechnology, to sea cucumber control enzymatic hydrolysis condition, the high sea cucumber polysaccharide of preparation bioavilability, adopt advanced technology again, be aided with natural botanical extraction liquid, sweetener and water and prepare the sea cucumber polysaccharide health drink.The compatibility raw material hawthorn of its natural botanical extraction liquid has hemangiectasis, cardiac stimulant, increase CF, improve the heart vigor, bring high blood pressure down and cholesterol, softening blood vessel, prevent and treat artery sclerosis, anti-aging, anticancer effect; That cassia seed has is hypotensive, the effect of reducing blood lipid and platelet aggregation-against; Honeysuckle has the effect of anti-inflammation, stimulating central nervous system system, reducing blood lipid.Its sweetener xylitol is a kind of sweetener, nutritious supplementary pharmaceutical and auxiliary therapeutical agent, it is the intermediate of human body carbohydate metabolism, lacking insulin in vivo influences under the glycometabolism situation, need not insulin promotes, xylitol also can permeate through cell membranes, be organized and absorb, promote liver glycogen synthetic, supply cell with nutrition and energy, and can not cause that blood glucose value raises, reduce the consumption of the protein in fat and the hepatic tissue, improve the effect that liver function and AFL form; Antierythrite belongs to low in calories, high-intensity sweeteners is not degraded by enzyme in vivo, can only see through kidney (easily by little intestinal absorption) and drain in the urine from blood and discharge, and not involved in sugar metabolism and change of blood sugar are so be suitable for patients with diabetes mellitus; FOS is a kind of natural active matter.Have the absorption of regulating gut flora, promoting calcium, the content that reduces cholesterol in serum and triglycerides, minimizing hepatotoxin and significant preventing cancer function, and pure taste is fragrant and sweet good to eat, has the adipose fragrance of class and tasty and refreshing soapy feeling.Therefore, sea cucumber polysaccharide health drink of the present invention, not only nutritious, clear, sour-sweet moderate, local flavor is natural, and has hypotensive, reducing blood lipid, improves liver function, regulates gut flora, strengthens health cares such as immunity, be fit to diabetic and wait special population to drink.
The specific embodiment
The present invention will be further described below in conjunction with embodiment.
Embodiment 1
A kind of sea cucumber polysaccharide preparation method of health-care beverage is through following technology
The preparation of a, sea cucumber polysaccharide
It is raw material that fresh sea cucumber is chosen in the processing of a1, raw material, sends into after cleaning and carries out fragmentation, homogenized in the tissue mashing machine;
A2, enzymolysis dilute the water that the sea cucumber milk of step a1 gained adds 5 times of its weight, and regulating pH value is 7.0, adding trypsase, wherein tryptic consumption is that every gram sea cucumber is added 3000 enzymes unit alive, the control temperature is 55 ℃, and enzymolysis 3h gets sea cucumber hydrolysate; With this sea cucumber hydrolysate boiling water bath, enzyme 15min goes out;
A3, alcohol precipitation go out step a2 gained, and to be evaporated to relative density for 50 ℃ be 1.02 for sea cucumber hydrolysate behind the enzyme, and the cooling back is 95% ethanol with the concentration of 2 times of amounts, and alcohol precipitation left standstill 24～36 hours; The control rotating speed is 4000r/min, centrifugal 10min, and collecting precipitation, 45 ℃ of dry processing of temperature control get sea cucumber polysaccharide, and are standby;
The preparation of b, natural botanical extraction liquid takes by weighing hawthorn 80%, cassia seed 10%, honeysuckle 10% by weight percentage, cleans, and adopts the steam water cooking method to process, and makes natural botanical extraction liquid more after filtration, and is standby;
The choose xylitol, antierythrite, FOS of c, sweetener are combined into sweetener, and be standby; It makes up by weight: xylitol: antierythrite: FOS=1: 1: 3;
Natural botanical extraction liquid 10kg, sweetener 10kg and the pure water 82kg that step c chooses of sea cucumber polysaccharide 8kg, the step b preparation of d, allotment weighing step a preparation mix;
E, can, sterilization are carried out can with the mixed liquor of steps d gained respectively by every jar of 250g, carry out sterilization processing, its sterilization mode again: intensification 10min, 105～115 ℃ of sterilization 15～40min, cooling 10min; Then it is cooled to central temperature less than 40 ℃, gets product.
Embodiment 2
A kind of sea cucumber polysaccharide preparation method of health-care beverage is characterized in that: through following technology
The preparation of a, sea cucumber polysaccharide
It is raw material that fresh sea cucumber is chosen in the processing of a1, raw material, sends into after cleaning and carries out fragmentation, homogenized in the tissue mashing machine;
A2, enzymolysis dilute the water that the sea cucumber milk of step a1 gained adds 3 times of its weight, and regulating pH value is 8.0, adding trypsase, wherein tryptic consumption is that every gram sea cucumber is added 2000 enzymes unit alive, the control temperature is 60 ℃, and enzymolysis 4h gets sea cucumber hydrolysate; With this sea cucumber hydrolysate boiling water bath, enzyme 20min goes out;
A3, alcohol precipitation go out step a2 gained, and to be evaporated to relative density for 55 ℃ be 1.05 for sea cucumber hydrolysate behind the enzyme, and the cooling back is 95% ethanol with the concentration of 2.5 times of amounts, and alcohol precipitation left standstill 24 hours; The control rotating speed is 3000r/min, centrifugal 12min, and collecting precipitation, 80 ℃ of dry processing of temperature control get sea cucumber polysaccharide, and are standby;
The preparation of b, natural botanical extraction liquid takes by weighing hawthorn 50%, cassia seed 30%, honeysuckle 20% by weight percentage, cleans, and adopts the steam water cooking method to process, and makes natural botanical extraction liquid more after filtration, and is standby;
The choose xylitol, antierythrite, FOS of c, sweetener are combined into sweetener, and be standby; It makes up by weight: xylitol: antierythrite: FOS=1: 1: 3;
Natural botanical extraction liquid 5kg, sweetener 15kg and the drinking water 70kg that step c chooses of sea cucumber polysaccharide 10kg, the step b preparation of d, allotment weighing step a preparation mix;
E, can, sterilization are carried out can with the mixed liquor of steps d gained respectively by every bottle of 400g, carry out sterilization processing, its sterilization mode again: intensification 10min, 105～115 ℃ of sterilization 15～40min, cooling 10min; Then it is cooled to central temperature less than 40 ℃, gets product.
Embodiment 3
A kind of sea cucumber polysaccharide preparation method of health-care beverage is characterized in that: through following technology
The preparation of a, sea cucumber polysaccharide
It is raw material that fresh sea cucumber is chosen in the processing of a1, raw material, sends into after cleaning and carries out fragmentation, homogenized in the tissue mashing machine;
A2, enzymolysis dilute the water that the sea cucumber milk of step a1 gained adds 10 times of its weight, and regulating pH value is 6.0, adding trypsase, wherein tryptic consumption is that every gram sea cucumber is added 4000 enzymes unit alive, the control temperature is 50 ℃, and enzymolysis 2h gets sea cucumber hydrolysate; With this sea cucumber hydrolysate boiling water bath, enzyme 10min goes out;
A3, alcohol precipitation go out step a2 gained, and to be evaporated to relative density for 60 ℃ be 1.2 for sea cucumber hydrolysate behind the enzyme, and the cooling back is 95% ethanol with the concentration of 3 times of amounts, and alcohol precipitation left standstill 30 hours; The control rotating speed is 2000r/min, centrifugal 15min, and collecting precipitation, 55 ℃ of dry processing of temperature control get sea cucumber polysaccharide, and are standby;
The preparation of b, natural botanical extraction liquid takes by weighing hawthorn 45%, cassia seed 25%, honeysuckle 30% by weight percentage, cleans, and adopts the steam water cooking method to process, and makes natural botanical extraction liquid more after filtration, and is standby;
The choose xylitol, antierythrite, FOS of c, sweetener are combined into sweetener, and be standby; It makes up by weight: xylitol: antierythrite: FOS=1: 1: 3;
Natural botanical extraction liquid 15kg, sweetener 5kg and the pure water 83kg that step c chooses of sea cucumber polysaccharide 2kg, the step b preparation of d, allotment weighing step a preparation mix;
E, can, sterilization are carried out can with the mixed liquor of steps d gained by every jar of 300g, carry out sterilization processing, its sterilization mode again: intensification 10min, 105～115 ℃ of sterilization 15～40min, cooling 10min; Then it is cooled to central temperature less than 40 ℃, gets product.
Embodiment 4
A kind of sea cucumber polysaccharide preparation method of health-care beverage is characterized in that: through following technology
The preparation of a, sea cucumber polysaccharide
It is raw material that fresh sea cucumber is chosen in the processing of a1, raw material, sends into after cleaning and carries out fragmentation, homogenized in the tissue mashing machine;
A2, enzymolysis dilute the water that the sea cucumber milk of step a1 gained adds 8 times of its weight, and regulating pH value is 7.5, adding trypsase, wherein tryptic consumption is that every gram sea cucumber is added 3500 enzymes unit alive, the control temperature is 60 ℃, and enzymolysis 2.5h gets sea cucumber hydrolysate; With this sea cucumber hydrolysate boiling water bath, enzyme 12min goes out;
A3, alcohol precipitation go out step a2 gained, and to be evaporated to relative density for 60 ℃ be 1.5 for sea cucumber hydrolysate behind the enzyme, and the cooling back is 95% ethanol with the concentration of 3 times of amounts, and alcohol precipitation left standstill 36 hours; The control rotating speed is 2500r/min, centrifugal 15min, and collecting precipitation, 60 ℃ of dry processing of temperature control get sea cucumber polysaccharide, and are standby;
The preparation of b, natural botanical extraction liquid takes by weighing hawthorn 60%, cassia seed 15%, honeysuckle 15% by weight percentage, cleans, and adopts the steam water cooking method to process, and makes natural botanical extraction liquid more after filtration, and is standby;
The choose xylitol, antierythrite, FOS of c, sweetener are combined into sweetener, and be standby; It makes up by weight: xylitol: antierythrite: FOS=1: 1: 3;
Natural botanical extraction liquid 10kg, sweetener 8kg and the pure water 72kg that step c chooses of sea cucumber polysaccharide 10kg, the step b preparation of d, allotment weighing step a preparation mix;
E, can, sterilization are carried out can with the mixed liquor of steps d gained by every jar of 250g, carry out sterilization processing, its sterilization mode again: intensification 10min, 105～115 ℃ of sterilization 15～40min, cooling 10min; Then it is cooled to central temperature less than 40 ℃, gets product.
Further specify the health care of sea cucumber polysaccharide health drink of the present invention below by reducing blood lipid drug effect experiment (preventative).
Be model with the high cholesterol rat, adopt the administering mode of oral administration gavage, observe the sea cucumber polysaccharide health drink to high cholesterol rat blood serum T-CHOL (TCH), the influence of triglycerides (TC) and HDL (HDL-C).
Experimental program is as follows:
72 of SPF level Wistar male rats, and body weight (200 ± 20g), available from Shandong Traditional Chinese Medicine University's Experimental Animal Center.After 72 Wistar rats were fed for 1 week with normal diet, free choice feeding conformed, and heart extracting blood behind the separation of serum, is measured wherein T-CHOL (TCH), the level of triglycerides (TG) and HDL-C (HDL-C).Being divided into 6 groups at random, is respectively the physiological saline group, the basic, normal, high dosage group of sea cucumber polysaccharide, positive controls and blank model group, 12 every group.1～5 group is high cholesterol emulsion group, wherein irritates stomach with the various dose sea cucumber polysaccharide 2～4 groups of every days and handles.The 1st group: high cholesterol emulsion+physiological saline; The 2nd group: high cholesterol emulsion+200mg/kg sea cucumber polysaccharide; The 3rd group: high cholesterol emulsion+400mg/kg sea cucumber polysaccharide; The 4th group: high cholesterol emulsion+800mg/kg sea cucumber polysaccharide; The 5th group: high cholesterol emulsion+2mg/kg simvastatin; The 6th group: the 400mg/kg sea cucumber polysaccharide.After irritating stomach 30d, fasting 12h, heart extracting blood, the centrifugal 15min of 3000g, separation of serum is measured T-CHOL (TCH) wherein, the concentration of triglycerides (TG) and HDL-C (HDL-C).High cholesterol emulsion: cholesterol 10%, lard 20%, NaTDC 15%, propylthiouracil 1%, glycerine 30%.Tween-80 5% adds water to 100%, prepares with high-shear emulsion machine.
Its sea cucumber polysaccharide health drink the results are shown in subordinate list to the influence of hyperlipemia rat blood lipid level.
Subordinate list sea cucumber polysaccharide health drink is to the influence (mmol/L) of hyperlipemia rat blood lipid level
*：P＜0.05
Show tentatively that according to pharmacodynamics the sea cucumber polysaccharide health drink has and reduces blood fat preferably.
Claims (1)
1. a sea cucumber polysaccharide preparation method of health-care beverage is characterized in that: through following technology
The preparation of a, sea cucumber polysaccharide
It is raw material that fresh sea cucumber is chosen in the processing of a1, raw material, sends into after cleaning and carries out fragmentation, homogenized in the tissue mashing machine;
A2, enzymolysis dilute the water that the sea cucumber milk of step a1 gained adds 3～10 times of its weight, regulating the pH value is 6.0～8.0, add trypsase, wherein tryptic consumption is that every gram sea cucumber is added 2000～4000 enzymes unit alive, the control temperature is 50～60 ℃, enzymolysis 2～4 h get sea cucumber hydrolysate; With this sea cucumber hydrolysate boiling water bath, enzyme 10～20 min go out;
A3, alcohol precipitation go out step a2 gained, and to be evaporated to relative density for 50～60 ℃ be 1～1.5 for sea cucumber hydrolysate behind the enzyme, and the cooling back is 95% ethanol with the concentration of 2～3 times of amounts, and alcohol precipitation left standstill 24～36 hours; The control rotating speed is 2000～4000 r/min, centrifugal 10～15 min, and collecting precipitation, 40～80 ℃ of dry processing of temperature control get sea cucumber polysaccharide, and are standby;
The preparation of b, natural botanical extraction liquid takes by weighing hawthorn 40～80%, cassia seed 10～30%, honeysuckle 10～30% by weight percentage, cleans, and adopts the steam water cooking method to process, and makes natural botanical extraction liquid more after filtration, and is standby;
The choose xylitol, antierythrite, FOS of c, sweetener are combined into sweetener, and be standby; It makes up by weight: Mu Tang Chun ︰ erythrose Chun ︰ FOS=1 ︰, 1 ︰ 3;
The sea cucumber polysaccharide of d, allotment weighing step a preparation, the natural botanical extraction liquid of step b preparation, sweetener and the water that step c chooses mix, the percentage by weight of each component is during weighing: sea cucumber polysaccharide 1～10%, natural botanical extraction liquid 5～15%, sweetener 5～15%, surplus are water;
E, can, sterilization are carried out can with the mixed liquor of steps d gained, carry out sterilization processing, its sterilization mode again: intensification 10min, 105～115 ℃ of sterilization 15～40 min, cool off 10 min; Then it is cooled to central temperature less than 40 ℃, gets product.
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| CN104068418B (en) * | 2013-03-28 | 2016-05-04 | 威海健方医药研究所 | A kind of sea cucumber drink production method that strengthens immunity |
| CN103704795A (en) * | 2013-11-28 | 2014-04-09 | 林淑琴 | Sea cucumber soup and manufacturing method thereof |
| CN103719193B (en) * | 2013-12-06 | 2016-05-18 | 山东好当家海洋发展股份有限公司 | A kind of sea cucumber brown alga nutrient cakes and preparation method thereof |
| CN104886583A (en) * | 2015-06-23 | 2015-09-09 | 青岛浩大海洋保健食品有限公司 | Composite scallop and holothurin liquid with blood fat regulating efficacy |
| CN104997024A (en) * | 2015-06-23 | 2015-10-28 | 青岛浩大海洋保健食品有限公司 | Composite scallop trepang liquid with immunity adjustment efficacy |
| CN104921125A (en) * | 2015-06-23 | 2015-09-23 | 青岛浩大海洋保健食品有限公司 | Dried scallop and trepang compound liquid having blood glucose reducing function |
| CN105011309A (en) * | 2015-07-05 | 2015-11-04 | 青岛浩大海洋保健食品有限公司 | Abalone and sea cucumber health-care drink |
| CN104997122A (en) * | 2015-08-14 | 2015-10-28 | 青岛浩大海洋保健食品有限公司 | Yin nourishing and yang strengthening maca compound health-care beverage |
| CN111789253A (en) * | 2020-07-07 | 2020-10-20 | 大连工业大学 | Method for preparing functional recombinant fish steak through 3D printing |
| CN113925142A (en) * | 2020-07-14 | 2022-01-14 | 天津星宇航天生物科技有限公司 | Nutritional formula suitable for astronauts to regulate high blood pressure, high blood sugar and improve immunity and preparation method thereof |
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Effective date of registration: 20161207 Address after: Tiger Tiger Town, Rongcheng city 264305 street Shandong city of Weihai province No. 1500 Patentee after: Shandong Haipu shield Biotechnology Co. Ltd. Address before: Homey Industrial Park in Shandong province Rongcheng City Tiger Town 264305 Patentee before: Shandong Homey Aquatic Development Co., Ltd. Patentee before: Biologic Inst., Shandong Prov. Academy of Sciences |