CN102524185A - Foam test tube platform for insect observation experiment and application method thereof - Google Patents
Foam test tube platform for insect observation experiment and application method thereof Download PDFInfo
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- CN102524185A CN102524185A CN2011104404265A CN201110440426A CN102524185A CN 102524185 A CN102524185 A CN 102524185A CN 2011104404265 A CN2011104404265 A CN 2011104404265A CN 201110440426 A CN201110440426 A CN 201110440426A CN 102524185 A CN102524185 A CN 102524185A
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Abstract
The invention discloses a foam test tube platform, which is prepared from foam plates as a raw material, and is composed of a bottom plate with grooves and a back plate with arc teeth. The foam test tube platform can be used for large-scale culturing of rice seedlings in a single plant in single tube manner and individual breeding and observation of rice planthopper, and has the advantages that the raw materials are easily obtained and low in price, the manufacturing process is simple and the foam test tube platform is convenient and practical.
Description
Technical field
The present invention relates to insect observation field, particularly a kind of foam test tube platform and application method thereof that is used for the insect observation experiment.
Background technology
Rice fulgorid (brown planthopper, white-backed planthopper and small brown rice planthopper etc.) and the paddy rice virus disease of propagating thereof have the characteristics of the diffusion of migrating at a distance, are the harmful insects that has a strong impact on Rice Production.Rice fulgorid not only causes damage to rice yield and quality through thorn suction rice juice, and the virus disease of its propagation can infect each breeding time of paddy rice, causes the popular and heavy losses of disease easily.For the virus disease of prevention and control rice fulgorid and propagation thereof, the biological property of research rice fulgorid, the biological mechanism of rice fulgorid transmitted virus and virus thereof are very important to the influence of rice fulgorid life parameters.
In order to observe the interference of other factors in statistics and the minimizing test exactly, generally select for use the single tube single head to raise and supply the examination rice fulgorid, fill the live body paddy rice seedling that nutrient solution is cultivated the same kind of same age in days in the pipe.Existing correlative study shows; Paddy rice virus generally passes without the rice fulgorid ovum, and the offspring of rice fulgorid must get food on strain just can obtain poison, so select the offspring of same female worm for use; Can reduce interindividual variation property, compare the otherness variation after rice fulgorid is felt poison more accurately; And the rice fulgorid population is got food on strain might not feel poison, reaches standard in order to guarantee the repeat number of feeling poisonous insect, must increase the experiment repeat number.Therefore, when single tube individual plant paddy rice seedling is raised the single head rice fulgorid, need to adopt large batch of large size test tube.And, require test tube to be positioned at heeling condition at the trial, if because test tube is an erectility; When the paddy rice seedling is put into test tube, owing to the paddy rice seedling that supports is kept upright, when inserting less insect individuality; Be difficult to it is adhered on the paddy rice seedling, be not easy again to climb on the paddy rice seedling and get food if receive on the test tube tube wall insect, and in experimentation; The paddy rice seedling all needs sufficient illumination with the growth that supplies the examination insect, and upright test tube is difficult to satisfy above-mentioned requirements.
Cultivation and the observation of the at present commercially available extremely inconvenient rice fulgorid of rack for test tube.At first, existing rack for test tube can only be fixed small size test tube, can not fix circular test tube of large batch of large size or erose test tube, and ability to bear is limited, and stability is limited; Secondly, existing rack for test tube can only make test tube upright, is unfavorable for invisible spectro paddy rice seedling contact tube wall, is unfavorable for that also the test-tube plantlet that is placed on same level goes to accept conditions such as illumination preferably with confession examination worm; Its three, existing rack for test tube is processed by wood or metal, cost is higher.
Summary of the invention
The objective of the invention is to overcome the shortcoming that exists in the prior art, the foam test tube platform that a kind of cost is low, can tilt to fix large size test tube in enormous quantities is provided.
Another object of the present invention is to provide a kind of method for using of above-mentioned foam test tube platform.
The object of the invention is realized through following technical proposals:
A kind of foam test tube platform, raw material are cystosepiments, are made up of base plate that has groove and the backup plate with arc tooth.
The arc tooth that is provided with on the groove that is provided with on the said base plate and the backup plate is corresponding each other, and the quantity of groove and arc tooth is more than ten.
Said base plate and backup plate are 90 ° of right angles, and base plate is wideer than backup plate.
Principle of the present invention is on base plate, to dig out groove with fixedly test tube bottom, vertically landing according to the diameter of test tube; On the inner side edge at backup plate top, dig out the arc tooth, make to correct on the test tube mouth to be fixed in the arc tooth, not cross deflection according to the caliber size of test tube.
The application method of above-mentioned foam test tube platform is that foam test tube platform is applied in phytotron or the climatic cabinate: (1) is applied to artificial climate when indoor, is foam test tube platform is positioned on the smooth desktop and parallel with fluorescent-lamp tube; Earlier test tube is placed on the arc tooth of foam test tube platform, this moment, test tube was the state of inclination, and the test tube face is towards light source; Pour nutrient solution into to each test tube; Then the paddy rice seedling is touched test tube wall and be placed on the mouth of pipe, and pressing the root of paddy rice seedling to slide to down gently at the bottom of the test tube in the nutrient solution, again test tube is placed on the foam test tube platform; Interval between the adjustment foam test tube platform; Make parallel rays just reach paddy rice rhizome boundary to the inverted image of front-seat test tube, every pipe inserts the rice fulgorid nymph in one 2 age, seals with gauze and rubber band; Its single female egg laying amount of observed and recorded, nymphal development progress and survival rate of difference nymph in the length of time and the life-span of adult, observed and recorded different disposal insect is to the degree situation that causes harm of individual plant paddy rice simultaneously; When (2) being applied in the climatic cabinate,, can utilize shelf in the climate box as base plate for the heeling condition of easier assurance test tube guarantees ventilation condition good in the climate box simultaneously; Rib on the shelf plays the effect of base plate upper groove, and foam test tube platform is only made backup plate part, and keeps at a certain distance away and dug the arc tooth; Make the phase adjacent tubes rigidly connect to touch and do not push, be placed on the shelf and with climate box in fluorescent tube vertical, equally test tube is placed on the arc tooth of backup plate; Inclined and the test tube face of test tube is towards the light source of respective side, and the same nutrient solution of pouring into is put into rice seedling; Insert nymph, seal observation.
The present invention compared with prior art has following advantage and effect:
(1) the present invention can tilt to fix the test tube platform of large size test tube in enormous quantities, efficiently solves the technical problem that single tube individual plant paddy rice seedling is cultivated and single head supplies the examination rice fulgorid to raise.
(2) raw material of the present invention obtain easily and are cheap, and manufacture craft is simple, and is convenient and practical.
Description of drawings
Fig. 1 is the structural representation of foam test tube platform of the present invention.
Fig. 2 is the sketch map of foam test tube platform of the present invention when tilting fixing large size test tube.
Fig. 3 for foam test tube platform of the present invention in the overall schematic that tilts during fixing test tube in enormous quantities.
Among the figure, 1, base plate; 2, arc tooth; 3, groove; 4, backup plate; 5, test tube.
Embodiment
Below in conjunction with embodiment the present invention is done further detailed description, but embodiment of the present invention is not limited thereto.
The preparation method of foam test tube platform: select the rice seedling about test tube individual plant water planting 10 ages in days of 25*200 specification for use.The base plate 1 and the backup plate 4 of foam test tube platform are 90 ° of right angles.Backup plate is long to be 990mm, and height is 75mm, and thick is 30mm; Base plate is long to be 990mm, and wide is 140mm, and thick is 45mm.On base plate, from backup plate one lateral edges 20mm place with test tube mouth back-off, the groove 3 of a diameter 30mm, dark 30mm is dug out in rotation.On the inner side edge at backup plate top, with the corresponding arc tooth 2 that diameter is 30mm, the tooth depth 10mm of plucking out of groove on the base plate.With as shown in Figure 1 in a row being placed on of making on the laboratory table of foam test tube platform, put into the test tube 5 of 25*200 specification, pipe is fixed in the groove at the end just, in the arc tooth, test tube is about 30 ° with respect to the inclination angle of base plate to test tube apart from the position of the about 5cm of the mouth of pipe.Every row can be put 25 in test tube; Pour the 2ml nutrient solution into to each test tube respectively, and the rice seedling about a strain 10 ages in days is touched tube wall be placed on the mouth of pipe, the root of pushing down rice seedling with writing brush slides to down in the pipe end nutrient solution gently; Interval between the adjustment foam test tube platform; Make parallel rays just reach paddy rice rhizome boundary to the inverted image of front-seat test tube, every pipe inserts the rice fulgorid nymph in one 2 age, seals with gauze and rubber band.
Use: the indoor condition of artificial climate is: air-conditioning is provided with 28 ℃ of temperature, humidifier adjustment humidity 70-80%, and illumination is common fluorescent lamp; Purpose is the poor opposite sex under the approaching in the controlled environment chamber natural condition, and experiment is provided with two processing, no poisonous insect and poisonous insect; The offspring of same female worm is after rice strain hatching, and a part directly is transferred to nontoxic rice seedling and carries out the raising of single tube single head, sets 40 repetitions; A part concentrates on earlier raises malicious 3d on the strain of tests positive; The rice seedling that transfers to the same kind of same age in days carries out the single tube single head raises, and sets 80, effectively repeats to be confirmed by final detection result.Adopt the foam test tube platform of present embodiment; Guarantee that on the one hand invisible spectro paddy rice can accept directional light fully and shine; Guarantee that on the other hand rice seedling contact tube wall is convenient to rice fulgorid and climbs up rice seedling and get food, also be convenient to directly observe rice seedling situation and rice fulgorid upgrowth situation simultaneously.
The preparation method of foam test tube platform: select the rice seedling about test tube individual plant water planting 10 ages in days of 25*200 specification for use, the climatic cabinate specification is LRH-550-GSI, interior case size 1100mm*540mm*910mm, and shelf is of a size of 930mm*360mm; Fluorescent tube is distributed in other three sides except door, in order to keep the inner good ventilation state of climate box, can utilize the interior shelf of climate box as base plate; Rib on the shelf plays the effect of base plate upper groove, only uses length to be 930mm, and height is 35mm; Thick is the backup plate of 25mm, and the backup plate two ends have more one section cystosepiment of about 20mm, is used for fixing to prevent that on the perpendicular rib of dividing plate both sides backup plate from sliding; The gap length of shelf plucks out the arc tooth in the corresponding climate box on backup plate, and diameter is 30mm, tooth depth 10mm; The test tube of putting into the 25*200 specification as shown in Figure 2, every row can be put 23 in test tube, and every layer of shelf can be put 46 in test tube; Totally 3 layers, the adjustment backup plate laterally is placed on the position apart from the horizontal rib 90mm in shelf the inside, about 30 ° of test tube angle of inclination; Make test tube face in a row just in time towards the direction of fluorescent tube, pour the 2ml nutrient solution respectively into, and the rice seedling about a strain 10 ages in days is touched tube wall be placed on the mouth of pipe; The root of pushing down rice seedling with writing brush slides to down in the pipe end nutrient solution gently, and every pipe inserts the rice fulgorid nymph in one 2 age, seals with gauze and rubber band.
Use: imposing a condition of climatic cabinate is 28 ℃ of temperature, humidity 80%, 6 grades of illumination; Purpose is the poor opposite sex under the condition of optimum rice fulgorid growth, and experiment is provided with two processing, no poisonous insect and poisonous insect; The offspring of same female worm is after rice strain hatching, and a part directly is transferred to nontoxic rice seedling and carries out the raising of single tube single head, sets 40 repetitions; A part concentrates on earlier after raising malicious 3d on the strain of tests positive; The rice seedling that transfers to the same kind of same age in days carries out the single tube single head raises, and sets 80, effectively repeats to be confirmed by final detection result.Adopt the foam test tube platform of present embodiment; To environmental condition in the climatic cabinate and interior; Done flexibly and transformed; The backup plate that only has the arc tooth on the one hand can guarantee that test tube is facing fluorescent tube but not the test tube mouth is creeped towards light to prevent rice fulgorid, has guaranteed also that on the other hand test tube is in that suitable angle of inclination is beneficial to rice growth and rice fulgorid is got food.
The preparation method of foam test tube platform: select the rice seedling about test tube individual plant water planting 10 ages in days of 25*200 specification for use, the climatic cabinate specification is GXZ-380A, interior case size 540mm*540mm*1300mm, and shelf is of a size of 430mm*480mm; Fluorescent-lamp tube is distributed in two sides, in order to keep the inner good ventilation state of climate box, can utilize the interior shelf of climate box as base plate, and the rib on the shelf plays the effect of base plate upper groove; Only use length to be 480mm, height is 35mm, and thick is the backup plate of 25mm; Backup plate just is stuck between the two horizontal horizontal ribs, and the distance beginning of distance one lateral edges 30mm plucks out the arc tooth about 1cm at interval successively from the backup plate, apart from backup plate opposite side edge 30mm; The arc tooth diameter is 30mm, and tooth depth 10mm puts into the test tube of 25*200 specification; Every row can be put 13 in test tube, and every layer of vertical two row of shelf can be put 26 in test tube; Totally 5 layers, the adjustment backup plate vertically is placed on the position apart from the perpendicular rib 80mm in shelf side, about 30 ° of test tube angle of inclination; Make test tube face in a row just in time towards the direction of fluorescent tube, pour the 2ml nutrient solution respectively into, and the rice seedling about a strain 10 ages in days is touched tube wall be placed on the mouth of pipe; The root of pushing down rice seedling with writing brush slides to down in the pipe end nutrient solution gently, and every pipe inserts the rice fulgorid nymph in one 2 age, seals with gauze and rubber band.
Use: imposing a condition of climatic cabinate is 25 ℃ or 31 ℃ of temperature, humidity 80%, illumination 4000LX; Purpose is the design temperature gradient ratio than rice fulgorid sense poison and the otherness of growth to temperature, draws temperature to rice fulgorid sense poison and the influence that passes poison, and the experiment of each temperature is provided with two processing; No poisonous insect and poisonous insect, the offspring of same female worm is after rice strain hatching, and a part directly is transferred to nontoxic rice seedling and carries out the raising of single tube single head; Set 40 repetitions, a part concentrates on earlier raises malicious 3d on the strain of tests positive, and the rice seedling that transfers to the same kind of same age in days carries out the single tube single head and raises; Set 80, effectively repeat to confirm by final detection result.Adopt the foam test tube platform of present embodiment; To environmental condition in the climatic cabinate and interior; Done flexibly and transformed; The backup plate that only has the arc tooth on the one hand can guarantee that test tube is facing fluorescent tube but not the test tube mouth is creeped towards light to prevent rice fulgorid, has guaranteed that on the other hand test tube is in that suitable angle of inclination is beneficial to rice growth and rice fulgorid is got food.
Claims (4)
1. foam test tube platform that is used for the insect observation experiment, it is characterized in that: raw material are cystosepiments, are made up of base plate that has groove and the backup plate with arc tooth.
2. foam test tube platform according to claim 1 is characterized in that: the arc tooth that is provided with on the groove that is provided with on the said base plate and the backup plate is corresponding each other, and the quantity of groove and arc tooth is more than ten.
3. foam test tube platform according to claim 1 is characterized in that: said base plate and backup plate are 90 ° of right angles, and base plate is wideer than backup plate.
4. application method that is used for the foam test tube platform of insect observation experiment; It is characterized in that: foam test tube platform is applied in phytotron or the climatic cabinate: (1) is applied to artificial climate when indoor, is foam test tube platform is positioned on the smooth desktop and parallel with fluorescent-lamp tube, earlier test tube is placed on the arc tooth of foam test tube platform; This moment, test tube was the state of inclination; The test tube face is poured nutrient solution towards light source into to each test tube, then the paddy rice seedling is touched test tube wall and is placed on the mouth of pipe; And the root of pressing the paddy rice seedling slides to down at the bottom of the test tube in the nutrient solution gently; Test tube is placed on the foam test tube platform, the interval between the adjustment foam test tube platform makes parallel rays just reach paddy rice rhizome boundary to the inverted image of front-seat test tube again; Every pipe inserts the rice fulgorid nymph in one 2 age; Seal with gauze and rubber band, its single female egg laying amount of observed and recorded, nymphal development progress and survival rate of difference nymph in the length of time and the life-span of adult, observed and recorded different disposal insect is to the degree situation that causes harm of individual plant paddy rice simultaneously; When (2) being applied in the climatic cabinate, as base plate, be the groove on the base plate with the rib on the shelf with the shelf in the climate box; On the backup plate of foam test tube platform, whenever keep at a certain distance away and dug the arc tooth, adjacent tubes is rigidly connected touch and do not push, be placed on the shelf and with climate box in fluorescent tube vertical; Test tube is placed on the arc tooth of backup plate, the inclined and test tube face of test tube is poured nutrient solution into towards the light source of respective side; Put into rice seedling, insert nymph, seal observation.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201110440426.5A CN102524185B (en) | 2011-12-23 | 2011-12-23 | Foam test tube platform for insect observation experiment and application method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201110440426.5A CN102524185B (en) | 2011-12-23 | 2011-12-23 | Foam test tube platform for insect observation experiment and application method thereof |
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| CN102524185A true CN102524185A (en) | 2012-07-04 |
| CN102524185B CN102524185B (en) | 2014-02-26 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110269048A (en) * | 2019-07-11 | 2019-09-24 | 河南工业大学 | The test device and method of continuous warm area test insect behavior under the high temperature conditions |
| CN110384075A (en) * | 2019-07-11 | 2019-10-29 | 河南工业大学 | Single water-saving essence of pump continous way mobility controls water-bath test device and method |
| CN110726719A (en) * | 2019-10-17 | 2020-01-24 | 广西贺州市桂东电子科技有限责任公司 | Device and method for detecting content of chloride ions in anodic formed foil electrolyte |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110269048A (en) * | 2019-07-11 | 2019-09-24 | 河南工业大学 | The test device and method of continuous warm area test insect behavior under the high temperature conditions |
| CN110384075A (en) * | 2019-07-11 | 2019-10-29 | 河南工业大学 | Single water-saving essence of pump continous way mobility controls water-bath test device and method |
| CN110269048B (en) * | 2019-07-11 | 2021-06-29 | 河南工业大学 | Testing device and method for testing behavior of insects under high-temperature condition by continuous temperature zone |
| CN110384075B (en) * | 2019-07-11 | 2021-06-29 | 河南工业大学 | Single-pump continuous type mobile water-saving precision control water bath testing device and method |
| CN110726719A (en) * | 2019-10-17 | 2020-01-24 | 广西贺州市桂东电子科技有限责任公司 | Device and method for detecting content of chloride ions in anodic formed foil electrolyte |
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| CN102524185B (en) | 2014-02-26 |
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Effective date of registration: 20160330 Address after: Moon Road, Science City high tech Industrial Development Zone of Guangzhou City, Guangdong province 510000 No. 80 science and technology innovation base D 607-609 unit 610-612 unit Patentee after: GUANGZHOU RUIFENG BIOTECHNOLOGY CO., LTD. Address before: 510642 Tianhe District, Guangdong, No. five road, No. 483, Patentee before: South China Agricultural University |
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| CB03 | Change of inventor or designer information |
Inventor after: Li Shenlei Inventor after: Zhang Maoxin Inventor after: Tu Zhi Inventor after: Ling Bing Inventor after: Zhou Guohui Inventor after: Jin Xin Inventor after: Lin Xiaojun Inventor after: Chen Fang Inventor after: Liu Huan Inventor before: Zhang Maoxin Inventor before: Tu Zhi Inventor before: Ling Bing Inventor before: Zhou Guohui |
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