CN102523920A - Preparation method of Grifola frondosa mycelial pellets and products thereof - Google Patents
Preparation method of Grifola frondosa mycelial pellets and products thereof Download PDFInfo
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- CN102523920A CN102523920A CN2012100059970A CN201210005997A CN102523920A CN 102523920 A CN102523920 A CN 102523920A CN 2012100059970 A CN2012100059970 A CN 2012100059970A CN 201210005997 A CN201210005997 A CN 201210005997A CN 102523920 A CN102523920 A CN 102523920A
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- pompon
- grifolas frondosa
- frondosa germ
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- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 240000001080 Grifola frondosa Species 0.000 title abstract description 15
- 235000007710 Grifola frondosa Nutrition 0.000 title abstract description 15
- 239000008188 pellet Substances 0.000 title abstract description 7
- 238000000855 fermentation Methods 0.000 claims abstract description 49
- 230000004151 fermentation Effects 0.000 claims abstract description 49
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 41
- 239000007788 liquid Substances 0.000 claims abstract description 25
- 235000015110 jellies Nutrition 0.000 claims abstract description 21
- 239000008274 jelly Substances 0.000 claims abstract description 21
- 235000013361 beverage Nutrition 0.000 claims abstract description 17
- 239000002775 capsule Substances 0.000 claims abstract description 14
- 241000222684 Grifola Species 0.000 claims description 110
- 241001388119 Anisotremus surinamensis Species 0.000 claims description 94
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 43
- 235000021552 granulated sugar Nutrition 0.000 claims description 31
- 238000004519 manufacturing process Methods 0.000 claims description 29
- 238000012807 shake-flask culturing Methods 0.000 claims description 18
- 235000015190 carrot juice Nutrition 0.000 claims description 13
- 239000002054 inoculum Substances 0.000 claims description 12
- 239000012530 fluid Substances 0.000 claims description 11
- 235000015197 apple juice Nutrition 0.000 claims description 10
- 238000010899 nucleation Methods 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 6
- 238000011068 loading method Methods 0.000 claims description 6
- 235000013175 Crataegus laevigata Nutrition 0.000 claims description 5
- 240000007087 Apium graveolens Species 0.000 claims description 3
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 claims description 3
- 235000010591 Appio Nutrition 0.000 claims description 3
- 240000007124 Brassica oleracea Species 0.000 claims description 3
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 claims description 3
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 claims description 3
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 claims description 3
- 244000241235 Citrullus lanatus Species 0.000 claims description 3
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 claims description 3
- 240000000111 Saccharum officinarum Species 0.000 claims description 3
- 235000007201 Saccharum officinarum Nutrition 0.000 claims description 3
- 240000001717 Vaccinium macrocarpon Species 0.000 claims description 3
- 235000012545 Vaccinium macrocarpon Nutrition 0.000 claims description 3
- 235000002118 Vaccinium oxycoccus Nutrition 0.000 claims description 3
- 235000004634 cranberry Nutrition 0.000 claims description 3
- 235000020379 cucumber juice Nutrition 0.000 claims description 3
- 235000015205 orange juice Nutrition 0.000 claims description 3
- 235000013944 peach juice Nutrition 0.000 claims description 3
- 235000015206 pear juice Nutrition 0.000 claims description 3
- 235000013948 strawberry juice Nutrition 0.000 claims description 3
- 235000015193 tomato juice Nutrition 0.000 claims description 3
- 239000000796 flavoring agent Substances 0.000 abstract description 8
- 235000019634 flavors Nutrition 0.000 abstract description 8
- 235000012055 fruits and vegetables Nutrition 0.000 abstract 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 230000008821 health effect Effects 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 230000035764 nutrition Effects 0.000 abstract 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 72
- 239000000047 product Substances 0.000 description 48
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 20
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 20
- 239000001630 malic acid Substances 0.000 description 20
- 235000011090 malic acid Nutrition 0.000 description 20
- 235000012907 honey Nutrition 0.000 description 18
- 241000222640 Polyporus Species 0.000 description 15
- 238000003556 assay Methods 0.000 description 12
- 239000012141 concentrate Substances 0.000 description 12
- 239000000843 powder Substances 0.000 description 12
- 230000001954 sterilising effect Effects 0.000 description 12
- 238000004659 sterilization and disinfection Methods 0.000 description 12
- 238000001035 drying Methods 0.000 description 8
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
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- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
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- 241000251468 Actinopterygii Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
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- 235000015165 citric acid Nutrition 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a preparation method of Grifola frondosa mycelial pellets and products thereof. The preparation method includes: using Grifola frondosa as an original strain for liquid fermentation; and obtaining Grifola frondosa mycelial pellets, wherein a culture medium is mainly composed of fruit and vegetable juice. The invention further provides products made of the Grifola frondosa mycelial pellets obtained by the preparation method, such as dried Grifola frondosa mycelial pellets, beverages, jelly, oral liquid and capsules. By using the fruit and vegetable juice as the culture medium, the prepared Grifola frondosa mycelial pellets and the products thereof have the advantages of natural and attractive color, unique flavor, rich nutrition and a certain health effect.
Description
Technical field
The present invention relates to preparation method of a kind of Grifolas frondosa germ pompon and products thereof.
Technical background
Edible mushroom is a kind of delicious flavour, nutritious health food, and its nutritive value is worth with health care and is mainly reflected in several like this aspects, the one, and protein content is high in the edible mushroom, and contains multiple essential amino acid; The 2nd, carbohydrate content is low in the edible mushroom, is a kind of food low in calories; The 3rd, unsaturated fatty acid content is high in the edible mushroom, the often edible urban disease that can prevent the modern times such as high fat of blood well; The 4th, contain the trace element and the multivitamin of multiple needed by human body such as phosphorus, iron in the edible mushroom; The 5th, edible mushroom contains a large amount of fungi polysaccharides, can effectively improve human immunological competence and anti-tumor capacity, and therefore, FAO's proposition 21 century, the most rational diet structure was " meat or fish one a plain mushroom ".
At present, all kinds of health products that at home and abroad are processed into the edible and medical fungi fruit body like various edible and medical fungi beverages, oral liquid, capsule etc., do not have still that to relate to the Juice be raw material, prepare method of Grifolas frondosa germ pompon and products thereof through solution fermentation.
Summary of the invention
The object of the present invention is to provide preparation method of a kind of Grifolas frondosa germ pompon and products thereof, is raw material with the Juice, prepares Grifolas frondosa germ pompon and products thereof through solution fermentation.
The present invention at first provides a kind of preparation method of Grifolas frondosa germ pompon, is starting strain with the grifola frondosus, carries out liquid fermentation, and the main component of medium is a Juice, obtains the Grifolas frondosa germ pompon.
Said liquid fermentation comprises and shakes a bottle enlarged culture, seeding tank enlarged culture and fermentation tank enlarged culture.
Said liquid fermentation may further comprise the steps:
(1) medium is formed: shake-flask culture base: Juice 50-300 ml, white granulated sugar 5-20g, KH
2PO
41-5g, MgSO
47H
2O 1-5g, ZnSO
47H
2O 0.1-0.5g adds water and is settled to 1000ml, the pH nature; Fermentation medium: Juice 50-300 ml, white granulated sugar 5-20g, KH
2PO
41-5g, MgSO
47H
2O 1-5g, ZnSO
47H
2O 0.1-0.5g, CaCO
33-7g adds water and is settled to 1000ml, pH pH nature;
(2) condition of culture:
One-level is shaken bottle: production inclined-plane kind is carried out enlarged culture with the shake-flask culture base, obtain producing and shake a bottle one-level kind, condition of culture is: with the 100-250mL/500mL loading amount, insert 0.5-2cm
2Slant strains, 70-250rpm shaken cultivation 5-10d, temperature 20-30 ℃;
Secondary shakes bottle: a bottle one-level kind is shaken in production use the inoculum concentration of volume ratio as 5-20%, by above-mentioned one-level shake-flask culture base and condition of culture, enlarged culture 3-7 days once more, obtain producing and shake a bottle secondary kind;
Seed tank culture: a bottle secondary kind is shaken in production transfer and carry out the enlarged culture one-level kind that obtains fermenting into the seeding tank that fermentation medium is housed; Condition of culture is: inoculum concentration 5-15% (v/v), warm 20-30 ℃ in jar, tank pressure 0.03-0.1MPa, mixing speed 50-200rpm, throughput 1:0.2-1.0v/v.min, fermented and cultured 3-7d;
Fermentation tank culture: by described fermentation medium of seed tank culture and condition of culture, fermentation one-level kind is transferred into the fermentation tank culture that fermentation medium is housed, ferment and put jar in 5-10 days, karusen obtains zymotic fluid and fresh Grifolas frondosa germ pompon through filtering.
Said Juice is one or more in must, apple juice, orange juice, peach juice, haw juice, pear juice, watermelon juice, sugar-cane juice, strawberry juice, cranberry juice, carrot juice, Fresh Cucumber Juice, tomato juice, cabbage juice, the Celery Juice.
The present invention also provides the Grifolas frondosa germ pompon that above-mentioned preparation method is obtained to process various Grifolas frondosa germ pompon dry products, beverage, jelly, oral liquid or capsule product.
Make medium with Juice, Grifolas frondosa germ pompon of preparation and products thereof has the characteristics of natural attractive in appearance, the unique flavor of color and luster, nutritious and certain function of health care.
Specific embodiment
The preparation method of a kind of Grifolas frondosa germ pompon of the present invention is a starting strain with the grifola frondosus, carries out liquid fermentation, and the main component of medium is a Juice, obtains the Grifolas frondosa germ pompon.
Said liquid fermentation comprises and shakes a bottle enlarged culture, seeding tank enlarged culture and fermentation tank enlarged culture.
Said liquid fermentation may further comprise the steps:
(1) medium is formed: shake-flask culture base: carrot juice 50-300 ml, white granulated sugar 5-20g, KH
2PO
41-5g, MgSO
47H
2O 1-5g, ZnSO
47H
2O 0.1-0.5g adds water and is settled to 1000ml, the pH nature; Fermentation medium: Juice 50-300 ml, white granulated sugar 5-20g, KH
2PO
41-5g, MgSO
47H
2O 1-5g, ZnSO
47H
2O 0.1-0.5g, CaCO
33-7g adds water and is settled to 1000ml, pH pH nature;
(2) condition of culture:
One-level is shaken bottle: production inclined-plane kind is carried out enlarged culture with the shake-flask culture base, obtain producing and shake a bottle one-level kind, condition of culture is: with the 100-250mL/500mL loading amount, insert 0.5-2cm
2Slant strains, 70-250rpm shaken cultivation 5-10d, temperature 20-30 ℃;
Secondary shakes bottle: a bottle one-level kind is shaken in production use the inoculum concentration of volume ratio as 5-20%, by above-mentioned one-level shake-flask culture base and condition of culture, enlarged culture 3-7 days once more, obtain producing and shake a bottle secondary kind;
Seed tank culture: a bottle secondary kind is shaken in production transfer and carry out the enlarged culture one-level kind that obtains fermenting into the seeding tank that fermentation medium is housed; Condition of culture is: inoculum concentration 5-15% (v/v), warm 20-30 ℃ in jar, tank pressure 0.03-0.1MPa, mixing speed 50-200rpm, throughput 1:0.2-1.0v/v.min, fermented and cultured 3-7d;
Fermentation tank culture: by described fermentation medium of seed tank culture and condition of culture, fermentation one-level kind is transferred into the fermentation tank culture that fermentation medium is housed, ferment and put jar in 5-10 days, karusen obtains zymotic fluid and fresh Grifolas frondosa germ pompon through filtering.
Said Juice is one or more in must, apple juice, orange juice, peach juice, haw juice, pear juice, watermelon juice, sugar-cane juice, strawberry juice, cranberry juice, carrot juice, Fresh Cucumber Juice, tomato juice, cabbage juice, the Celery Juice.
Grifolas frondosa germ pompon by above-mentioned preparation method obtains is processed various Grifolas frondosa germ pompon dry products, beverage, jelly, oral liquid or capsule product, and specific product is following:
1. Grifolas frondosa germ pompon product
1.1 Grifolas frondosa germ pompon dry product
Fresh Grifolas frondosa germ pompon obtains Grifolas frondosa germ pompon dry product after heated-air drying or freeze drying or microwave drying.
1.2 Grifolas frondosa germ pompon suspending beverage
Prescription: Grifolas frondosa germ pompon dry product (a kind or several kinds) 2-5% or fresh Grifolas frondosa germ pompon (a kind or several kinds) 10-25%; Acid such as citric acid, malic acid 0.08%-0.15%; Flavour of candy agent such as white granulated sugar 3-9%, honey 0-3%; Juice 0-15%, zymotic fluid 0-10% (a kind or several kinds) add pure water to 100%.
Production technology: Grifolas frondosa germ pompon dry product (a kind or several kinds) 2-5% or fresh Grifolas frondosa germ pompon (a kind or several kinds) 10-25% add the 1-3 pure water and boiled 10-15 minute; Add acid 0.08%-0.15% such as citric acid, malic acid then, flavour of candy agent such as white granulated sugar 3-9%, honey 0-3%, Juice 0-15%, zymotic fluid 0-10% (a kind or several kinds) add pure water to 100%, mixing preparation, with the citric acid adjust pH to 3.2-4.0; Beverage bottling while hot, capping, sterilization that allotment is good are product through after the assay was approved.
1.3 Grifolas frondosa germ pompon jelly
Prescription: Grifolas frondosa germ pompon dry product (a kind or several kinds) 2-5% or fresh Grifolas frondosa germ pompon (a kind or several kinds) 10-25%; Jelly powder 0.3-0.8%; Acid such as citric acid, malic acid 0.08%-0.15%; Flavour of candy agent such as white granulated sugar 3-9%, honey 0-3%, Juice 0-10%, zymotic fluid 0-10% (a kind or several kinds) add pure water to 100%.
Production technology: Grifolas frondosa germ pompon dry product (a kind or several kinds) 2-5% or fresh Grifolas frondosa germ pompon (a kind or several kinds) 10-25% add the 1-3 pure water and boiled 10-15 minute; Add jelly powder 0.3-0.8% then, stirring and dissolving continues heating 3-5 minute; Add acid 0.08%-0.15% such as citric acid, malic acid, flavour of candy agent such as white granulated sugar 3-9%, honey 0-3%, Juice 0-15%, zymotic fluid 0-10% (a kind or several kinds) add pure water to 100%, stir; Grifolas frondosa germ pompon jelly package, sterilization that allotment is good are product through after the assay was approved.
1.4 Grifola frondosa oral liquid, capsule
Grifolas frondosa germ pompon dry product (a kind or several kinds) or fresh Grifolas frondosa germ pompon (a kind or several kinds) are added the water of 2-5 times of weight, put in the 95-100 ℃ of water-bath and extract 0.5-1h, get extract A and filter residue through plate compression; This filter residue is repeated to extract, filter 1-2 time again, get extract B, merge zymotic fluid, extract A and extract B, 40-75 ℃, it is 3-10% that the 0.05-0.1MPa cryogenic vacuum is concentrated into soluble solid, gets concentrate.
Said concentrate is boiled; Behind the centrifugal removal insoluble matter wherein, by weight adding acid 0.08%-0.15% such as citric acid, malic acid, white granulated sugar 3-9%, honey 0-3% etc.; Oral liquid bottling, sterilization that allotment is good are the Grifola frondosa oral liquid product through after the assay was approved.
With said concentrate atomized drying, get grifola frondosus extract dry powder, adopt prior art to process the grifola frondosus capsule.
For fully disclosing preparation method of a kind of Grifolas frondosa germ pompon of the present invention and products thereof, explain below in conjunction with embodiment.
Embodiment 1:
1. the preparation of Grifolas frondosa germ pompon
1.1 the grifola frondosus conserving species is carried out activation, rejuvenation with potato culture (PDA medium), put 26 ℃ of static cultivations of insulating box 7 days, obtain production inclined-plane kind, put refrigerator and cooled and hide subsequent use; The prescription of described PDA medium is (g/L): peeling potato 200, glucose 15, agar 20, add water be settled to volume required, pH nature;
1.2 production inclined-plane kind is carried out enlarged culture with the shake-flask culture base, obtains producing and shake a bottle one-level kind; The prescription of described shake-flask culture base be (/L): haw juice 50ml, carrot juice 50ml, white granulated sugar 5g, KH
2PO
41g, MgSO
47H
2O 2g, ZnSO
47H
2O 0.1g adds water constant volume 1000 ml, the pH nature; Condition of culture is: with the 100mL/500mL loading amount, insert 1cm
2Slant strains, rotary shaking table 250rpm cultivates 5d, 30 ℃ of temperature;
1.3 production is shaken bottle one-level kind with 5% inoculum concentration, 1.2 described shake-flask culture based formulas and condition of culture set by step, enlarged culture is 3 days once more, must produce and shake a bottle secondary kind;
1.4 a bottle secondary kind is shaken in production transfers and carries out the enlarged culture one-level kind that obtains fermenting into the seeding tank that fermentation medium is housed; Described fermentative medium formula be (/L): haw juice 50ml, carrot juice 50ml, white granulated sugar 5g, KH
2PO
41g, MgSO
47H
2O 2g, ZnSO
47H
2O 0.1g, CaCO
33g adds water and is settled to 1000 ml, the pH nature; Condition of culture is: inoculum concentration 5%, 30 ℃ of jar temperature, tank pressure 0.1MPa, speed of agitator 200rpm, throughput 1:1v/v.min, fermented and cultured 5d;
1.5 1.4 described fermentative medium formula and condition of culture transfer fermentation one-level kind into the fermentation tank culture that fermentation medium is housed set by step, ferment and put jar in 5 days, karusen obtains polyporus frondosus fermenting liquor and fresh Grifolas frondosa germ pompon through filtering.
2. Grifolas frondosa germ pompon product
2.1 Grifolas frondosa germ pompon dry product
Fresh Grifolas frondosa germ pompon obtains Grifolas frondosa germ pompon dry product after heated-air drying or freeze drying or microwave drying.
2.2 Grifolas frondosa germ pompon suspending beverage
Prescription: fresh Grifolas frondosa germ pompon 20%, citric acid 0.05%, malic acid 0.03%, white granulated sugar 8%, carrot juice 5%, polyporus frondosus fermenting liquor 5% add pure water to 100%.
Production technology: fresh Grifolas frondosa germ pompon 20% adds 2 times of pure water and boiled 10-15 minute; Add citric acid 0.05%, malic acid 0.03% then, granulated sugar 8%, carrot juice 5%, polyporus frondosus fermenting liquor 5% add pure water to 100%, mixing preparation, with the citric acid adjust pH to 3.2-4.0; The bottling while hot of Grifolas frondosa germ pompon suspending beverage, capping, sterilization that allotment is good are Grifolas frondosa germ pompon suspending beverage finished product through after the assay was approved.
2.3 Grifolas frondosa germ pompon jelly
Prescription: Grifolas frondosa germ pompon dry product 3%, jelly powder 0.5%, citric acid 0.05%, malic acid 0.03%, white granulated sugar 6%, honey 0.5%, carrot juice 5%, polyporus frondosus fermenting liquor 5% add pure water to 100%.
Production technology: Grifolas frondosa germ pompon dry product 3% adds 3 times of pure water and boiled 15 minutes; Add jelly powder 0.5% then, stirring and dissolving continues heating 5 minutes; Add citric acid 0.05%, malic acid 0.03%, white granulated sugar 6%, honey 0.5%, carrot juice 5%, polyporus frondosus fermenting liquor 5% add pure water to 100%, stir; Grifolas frondosa germ pompon jelly package, sterilization that allotment is good are product through after the assay was approved.
2.4 Grifola frondosa oral liquid, capsule
With the water of fresh 2 times of weight of Grifolas frondosa germ pompon adding, put in 100 ℃ of water-baths and extract 0.5h, get extract A and filter residue through plate compression; This filter residue is repeated to extract, filter 1 time again, get extract B, merge zymotic fluid, extract A and extract B, 55 ℃, it is 10% that the 0.05-0.1MPa cryogenic vacuum is concentrated into soluble solid, gets concentrate.
Said concentrate is boiled; Behind the centrifugal removal insoluble matter wherein, by weight adding citric acid 0.05%, malic acid 0.03%, white granulated sugar 6%, honey 0.5% etc.; Oral liquid bottling, sterilization that allotment is good are the Grifola frondosa oral liquid product through after the assay was approved.
With said concentrate atomized drying, get grifola frondosus extract dry powder, adopt prior art to process the grifola frondosus capsule.
Embodiment 2:
1. the preparation of Grifolas frondosa germ pompon
1.1 the grifola frondosus conserving species is carried out activation, rejuvenation with potato culture (PDA medium), put 28 ℃ of static cultivations of insulating box 7 days, obtain production inclined-plane kind, put refrigerator and cooled and hide subsequent use; The prescription of described PDA medium is (g/L): peeling potato 250, glucose 15, agar 15, add water be settled to volume required, pH nature;
1.2 production inclined-plane kind is carried out enlarged culture with the shake-flask culture base, obtains producing and shake a bottle one-level kind; The prescription of described shake-flask culture base be (/L): carrot juice 100ml, must 50 ml, white granulated sugar 5 g, KH
2PO
42g, MgSO
47H
2O 2g, ZnSO
47H
2O 0.25g adds water constant volume 1000 ml, the pH nature; Condition of culture is: with the 250mL/500mL loading amount, insert 1cm
2Slant strains, rotary shaking table 120rpm cultivates 7d, 28 ℃ of temperature;
1.3 production is shaken bottle one-level kind with 10% inoculum concentration, 1.2 described shake-flask culture based formulas and condition of culture set by step, enlarged culture is 5 days once more, must produce and shake a bottle secondary kind;
1.4 a bottle secondary kind is shaken in production transfers and carries out the enlarged culture one-level kind that obtains fermenting into the seeding tank that fermentation medium is housed; Described fermentative medium formula be (/L): carrot juice 100ml, must 50 ml, white granulated sugar 5 g, KH
2PO
42g, MgSO
47H
2O 2g, ZnSO
47H
2O 0.25g, CaCO
33g adds water and is settled to volume requiredly, and pH 6.5; Condition of culture is: inoculum concentration 10%, 28 ℃ of jar temperature, tank pressure 0.05MPa, speed of agitator 120rpm, throughput 1:0.5v/v.min, fermented and cultured 5d;
1.5 1.4 described fermentative medium formula and condition of culture transfer fermentation one-level kind into the fermentation tank culture that fermentation medium is housed set by step, ferment and put jar in 7 days, karusen obtains polyporus frondosus fermenting liquor and fresh Grifolas frondosa germ pompon through filtering.
2. Grifolas frondosa germ pompon product
2.1 Grifolas frondosa germ mycelium pellet dry product
Fresh Grifolas frondosa germ pompon obtains Grifolas frondosa germ pompon dry product after heated-air drying or freeze drying or microwave drying.
2.2 Grifolas frondosa germ pompon suspending beverage
Prescription: fresh Grifolas frondosa germ pompon 20%, citric acid 0.06%, malic acid 0.02%, white granulated sugar 5%, honey 1%, must 3%, polyporus frondosus fermenting liquor 6% add pure water to 100%.
Production technology: fresh Grifolas frondosa germ pompon 20% adds 2 times of pure water and boiled 10-15 minute; Add citric acid 0.06%, malic acid 0.02% then, granulated sugar 5%, honey 1%, must 3%, polyporus frondosus fermenting liquor 6% add pure water to 100%, mixing preparation, with the citric acid adjust pH to 3.2-4.0; The bottling while hot of Grifolas frondosa germ pompon suspending beverage, capping, sterilization that allotment is good are Grifolas frondosa germ pompon suspending beverage finished product through after the assay was approved.
2.3 Grifolas frondosa germ pompon jelly
Prescription: Grifolas frondosa germ pompon dry product 3%, jelly powder 0.5%, citric acid 0.06%, malic acid 0.02%, white granulated sugar 5%, honey 1%, must 3%, polyporus frondosus fermenting liquor 6% add pure water to 100%.
Production technology: Grifolas frondosa germ pompon dry product 3% adds 3 times of pure water and boiled 15 minutes; Add jelly powder 0.5% then, stirring and dissolving continues heating 5 minutes; Add citric acid 0.05%, malic acid 0.03%, white granulated sugar 5%, honey 1%, must 3%, polyporus frondosus fermenting liquor 6% add pure water to 100%, stir; Grifolas frondosa germ pompon jelly package, sterilization that allotment is good are product through after the assay was approved.
2.4 Grifola frondosa oral liquid, capsule
With the water of fresh 2 times of weight of Grifolas frondosa germ pompon adding, put in 100 ℃ of water-baths and extract 0.5h, get extract A and filter residue through plate compression; This filter residue is repeated to extract, filter 1 time again, get extract B, merge zymotic fluid, extract A and extract B, 55 ℃, it is 10% that the 0.1MPa cryogenic vacuum is concentrated into soluble solid, gets concentrate.
Said concentrate is boiled, and behind the centrifugal removal insoluble matter wherein, by weight adding citric acid 0.06%, malic acid 0.02%, white granulated sugar 5%, honey 1% etc. with allotment oral liquid bottling, sterilization well, are the Grifola frondosa oral liquid product through after the assay was approved.
With said concentrate atomized drying, get grifola frondosus extract dry powder, adopt prior art to process the grifola frondosus capsule.
Embodiment 3:
1. the preparation of Grifolas frondosa germ pompon
1.1 the grifola frondosus conserving species is carried out activation, rejuvenation with potato culture (PDA medium), put 30 ℃ of static cultivations of insulating box 7 days, obtain production inclined-plane kind, put refrigerator and cooled and hide subsequent use; The prescription of described PDA medium is (g/L): peeling potato 150, glucose 20, agar 15, add water be settled to volume required, pH nature;
1.2 production inclined-plane kind is carried out enlarged culture with the shake-flask culture base, obtains producing and shake a bottle one-level kind; The prescription of described shake-flask culture base be (/L): apple juice 50 ml, white granulated sugar 10 g, KH
2PO
45g, MgSO
47H
2O 5g, ZnSO
47H
2O 0.5g adds water constant volume 1000 ml, the pH nature; Condition of culture is: with the 250mL/500mL loading amount, insert 1cm
2Slant strains, rotary shaking table 70rpm cultivates 10d, 20 ℃ of temperature;
1.3 production is shaken bottle one-level kind with 10% inoculum concentration, 1.2 described shake-flask culture based formulas and condition of culture set by step, enlarged culture is 7 days once more, must produce and shake a bottle secondary kind;
1.4 a bottle secondary kind is shaken in production transfers and carries out the enlarged culture one-level kind that obtains fermenting into the seeding tank that fermentation medium is housed; Described fermentative medium formula be (/L): apple juice 50 ml, KH
2PO
45g, MgSO
47H
2O 5g, ZnSO
47H
2O 0.5g, CaCO
35g, add water be settled to volume required, pH nature; Condition of culture is: inoculum concentration 15%, 20 ℃ of jar temperature, tank pressure 0.03MPa, speed of agitator 50rpm, throughput 1:0.2v/v.min, fermented and cultured 7d;
1.5 1.4 described fermentative medium formula and condition of culture transfer fermentation one-level kind into the fermentation tank culture that fermentation medium is housed set by step, ferment and put jar in 10 days, karusen obtains polyporus frondosus fermenting liquor and fresh Grifolas frondosa germ pompon through filtering.
2. Grifolas frondosa germ pompon product
2.1 Grifolas frondosa germ pompon dry product
Fresh Grifolas frondosa germ pompon obtains Grifolas frondosa germ pompon dry product after heated-air drying or freeze drying or microwave drying.
2.2 Grifolas frondosa germ pompon suspending beverage
Prescription: fresh Grifolas frondosa germ pompon 15%, citric acid 0.06%, malic acid 0.02%, white granulated sugar 5%, honey 0.5%, apple juice 3%, polyporus frondosus fermenting liquor 5% add pure water to 100%.
Production technology: fresh Grifolas frondosa germ pompon 15% adds 2 times of pure water and boiled 10-15 minute; Add citric acid 0.06%, malic acid 0.02% then, granulated sugar 5%, honey 0.5%, apple juice 3%, polyporus frondosus fermenting liquor 5% add pure water to 100%, mixing preparation, with the citric acid adjust pH to 3.2-4.0; The bottling while hot of Grifolas frondosa germ pompon suspending beverage, capping, sterilization that allotment is good are Grifolas frondosa germ pompon suspending beverage finished product through after the assay was approved.
2.3 Grifolas frondosa germ pompon jelly
Prescription: Grifolas frondosa germ pompon dry product 3%, jelly powder 0.5%, citric acid 0.06%, malic acid 0.02%, white granulated sugar 5%, honey 0.5%, apple juice 3%, polyporus frondosus fermenting liquor 5% add pure water to 100%.
Production technology: Grifolas frondosa germ pompon dry product 3% adds 3 times of pure water and boiled 15 minutes; Add jelly powder 0.5% then, stirring and dissolving continues heating 5 minutes; Add citric acid 0.05%, malic acid 0.03%, white granulated sugar 5%, honey 0.5%, apple juice 3%, polyporus frondosus fermenting liquor 5% add pure water to 100%, stir; Grifolas frondosa germ pompon jelly package, sterilization that allotment is good are product through after the assay was approved.
2.4 Grifola frondosa oral liquid, capsule
With the water of fresh 2 times of weight of Grifolas frondosa germ pompon adding, put in 100 ℃ of water-baths and extract 0.5h, get extract A and filter residue through plate compression; This filter residue is repeated to extract, filter 1 time again, get extract B, merge zymotic fluid, extract A and extract B, 55 ℃, it is 10% that the 0.1MPa cryogenic vacuum is concentrated into soluble solid, gets concentrate.
Said concentrate is boiled, and behind the centrifugal removal insoluble matter wherein, by weight adding citric acid 0.06%, malic acid 0.02%, white granulated sugar 5%, honey 1% etc. with allotment oral liquid bottling, sterilization well, are the Grifola frondosa oral liquid product through after the assay was approved.
With said concentrate atomized drying, get grifola frondosus extract dry powder, adopt prior art to process the grifola frondosus capsule.
Preparation method of Grifolas frondosa germ pompon and products thereof is a raw material with Juices such as carrot juice, must, apple juices, is starting strain with the grifola frondosus; Carry out liquid fermentation, obtain a large amount of Grifolas frondosa germ pompons, then with Grifolas frondosa germ pompon major ingredient; Process products such as various Grifolas frondosa germ pompon dry products, beverage, jelly, oral liquid, capsule; Product has nutritious, unique flavor, and color and luster is natural attractive in appearance and the characteristics of certain function of health care are arranged.
Claims (5)
1. the preparation method of a Grifolas frondosa germ pompon, it is characterized in that: be starting strain with the grifola frondosus, carry out liquid fermentation, the main component of medium is a Juice, obtains the Grifolas frondosa germ pompon.
2. the preparation method of Grifolas frondosa germ pompon according to claim 1 is characterized in that: said liquid fermentation comprises and shakes a bottle enlarged culture, seeding tank enlarged culture and fermentation tank enlarged culture.
3. the preparation method of Grifolas frondosa germ pompon according to claim 2 is characterized in that: said liquid fermentation may further comprise the steps:
(1) medium is formed: shake-flask culture base: Juice 50-300 ml, white granulated sugar 5-20g, KH
2PO
41-5g, MgSO
47H
2O 1-5g, ZnSO
47H
2O 0.1-0.5g adds water and is settled to 1000ml, the pH nature; Fermentation medium: Juice 50-300 ml, white granulated sugar 5-20g, KH
2PO
41-5g, MgSO
47H
2O 1-5g, ZnSO
47H
2O 0.1-0.5g, CaCO
33-7g adds water and is settled to 1000ml, the pH nature;
(2) condition of culture:
One-level is shaken bottle: production inclined-plane kind is carried out enlarged culture with the shake-flask culture base, obtain producing and shake a bottle one-level kind, condition of culture is: with the 100-250mL/500mL loading amount, insert 0.5-2cm
2Slant strains, 70-250rpm shaken cultivation 5-10d, temperature 20-30 ℃;
Secondary shakes bottle: a bottle one-level kind is shaken in production use the inoculum concentration of volume ratio as 5-20%, by above-mentioned one-level shake-flask culture base and condition of culture, enlarged culture 3-7 days once more, obtain producing and shake a bottle secondary kind;
Seed tank culture: a bottle secondary kind is shaken in production transfer and carry out the enlarged culture one-level kind that obtains fermenting into the seeding tank that fermentation medium is housed; Condition of culture is: inoculum concentration 5-15% (v/v), warm 20-30 ℃ in jar, tank pressure 0.03-0.1MPa, mixing speed 50-200rpm, throughput 1:0.2-1.0v/v.min, fermented and cultured 3-7d;
Fermentation tank culture: by described fermentation medium of seed tank culture and condition of culture, fermentation one-level kind is transferred into the fermentation tank culture that fermentation medium is housed, ferment and put jar in 5-10 days, karusen obtains zymotic fluid and fresh Grifolas frondosa germ pompon through filtering.
4. the preparation method of Grifolas frondosa germ pompon according to claim 1 is characterized in that: said Juice is one or more in must, apple juice, orange juice, peach juice, haw juice, pear juice, watermelon juice, sugar-cane juice, strawberry juice, cranberry juice, carrot juice, Fresh Cucumber Juice, tomato juice, cabbage juice, the Celery Juice.
5. Grifolas frondosa germ pompon product, it is characterized in that: the Grifolas frondosa germ pompon that utilizes claim 1,2,3 or 4 said preparation methods to obtain is processed various Grifolas frondosa germ pompon dry products, beverage, jelly, oral liquid or capsule product.
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| CN104145711A (en) * | 2014-04-18 | 2014-11-19 | 湖南省春华生物科技有限公司 | Grifola frondosa three-stage culture production method |
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| CN107629964A (en) * | 2017-08-29 | 2018-01-26 | 上海雪榕生物科技股份有限公司 | A kind of preparation method of Grifolas frondosa germ pompon and products thereof |
| CN108034593A (en) * | 2018-01-31 | 2018-05-15 | 北京农学院 | A kind of fungi culture medium and its application |
| CN108497153A (en) * | 2018-03-01 | 2018-09-07 | 广州大学 | A kind of Ganoderma Lucidum pompon chewing gum and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107629964A (en) * | 2017-08-29 | 2018-01-26 | 上海雪榕生物科技股份有限公司 | A kind of preparation method of Grifolas frondosa germ pompon and products thereof |
| CN108034593A (en) * | 2018-01-31 | 2018-05-15 | 北京农学院 | A kind of fungi culture medium and its application |
| CN108034593B (en) * | 2018-01-31 | 2021-01-29 | 北京农学院 | Fungus culture medium and application thereof |
| CN108497153A (en) * | 2018-03-01 | 2018-09-07 | 广州大学 | A kind of Ganoderma Lucidum pompon chewing gum and preparation method thereof |
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| CN102523920B (en) | 2013-12-04 |
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