CN102512541B - Compound mulberry preparation capable of improving superoxide dismutase activity - Google Patents
Compound mulberry preparation capable of improving superoxide dismutase activity Download PDFInfo
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- CN102512541B CN102512541B CN2012100099200A CN201210009920A CN102512541B CN 102512541 B CN102512541 B CN 102512541B CN 2012100099200 A CN2012100099200 A CN 2012100099200A CN 201210009920 A CN201210009920 A CN 201210009920A CN 102512541 B CN102512541 B CN 102512541B
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Abstract
The invention belongs to the technical field of processing of health-care products and relates to a compound mulberry preparation capable of improving superoxide dismutase activity. The preparation method for the compound mulberry preparation comprises the following steps of: extracting 12g of mulberry and 9g of medlar in ethanol of which the concentration is 70 percent and the amount is 10 times that of the mulberry and the medlar at room temperature for 10 hours, sealing and crushing the extract with ultrasonic waves for 30 minutes, filtering the extract, and extracting the filtration residue with ethanol of which the concentration is 70 percent and the amount is 6 times and 4 times that of the filtration residue again for one time respectively; combining the filtrates, reclaiming the ethanol to obtain an extract of the mulberry and medlar; centrifuging 20g of porphyridium cruentum with a centrifugal for 10 minutes, suspending the porphyridium cruentum in 50mmol/L of phosphate buffer of which the pH value is 7.4, crushing the porphyridium cruentum with ultrasonic waves, centrifuging the crushed porphyridium cruentum with a high-speed centrifugal for 15 minutes to obtain a liquid supernatant, and refrigerating and drying the liquid supernatant to obtain a dried product; and uniformly mixing the dried product and the extract of the mulberry and medlar to obtain the mulberry preparation. The mulberry preparation can be applied to a natural antioxidant which is newly developed in fields, such as food, medicament and the like, and contributes to industrial production.
Description
Technical field
Health food processing technique field under the present invention the present invention relates to a kind of compound recipe Fructus Mori preparation that can improve superoxide dismutase activity.Product of the present invention can be used for the new Natural antioxidants of field exploitation such as food, medicine, and product of the present invention is beneficial to suitability for industrialized production.
Background technology
Fructus Mori is the dry fruit ear of moraceae plants Mulberry Morus alba L..Sweet, sour, cold.GUIXIN, liver, kidney channel.The YIN nourishing of enriching blood is promoted the production of body fluid and is moisturized.Contain glucose, fructose, tannin, malic acid, rutin, anthocyanin, carotene, Yan acid, linoleic acid, vitamin B, vitamin C, lymphatic temperament, cyanidin, calcareous etc.But the weight of modern pharmacology proof enhance immunity organ, can strengthen the nonspecific immunity function, humoral immunization is had the increase effect, the cell-mediated immunologic function of T is had significant facilitation, cell mutation is had preventive effect, have effect and the anti-AIDS effect of anti-hepatitis B virus.
Fructus Lycii is the dry mature fruit of plant of Solanaceae lycium barbarum Lycium barbarum L..Its sweet in the mouth, property are put down.Effect with the liver and the kidney tonifying.Modern pharmacology studies confirm that Fructus Lycii scalable body's immunity, can effectively suppress tumor growth and cell mutation, has slow down aging, the effect of anti-fatty liver, adjusting blood fat and aspects such as blood glucose, promotion hemopoietic function.
Porphyridium cruentum (Porphyridium cruentum) is a kind of unicellular red algae that belongs to Rhodophyta, can synthesize bioactive substances such as polysaccharide sulfate, phycobniliprotein and polyunsaturated fatty acid outside the born of the same parents in a large number in growth course.The Porphyridium cruentum phycoerythrin accounts for phycobniliprotein 84%, and is wherein maximum with B-phycoerythrin content.Phycoerythrin is of many uses, it both can be used as natural pigment and had been applied to industry such as food, cosmetics, dyestuff, can be made into fluorometric reagent again and be used for clinical diagnose and fields such as immunochemistry and biological engineering, phycoerythrin still is a kind of photosensitizer with potentiality to be exploited, is used for the optical dynamic therapy of tumor.The outer sulfated polysaccharides ntiviral characteristic of the born of the same parents of Porphyridium cruentum is outstanding, has functions such as antitumor, antiallergic, antioxidation, blood fat reducing, accent blood fat.Therefore, comprehensive exploitation Porphyridium cruentum phycoerythrin, polysaccharide and algae powder product series, economic results in society are obvious.
The generation development of oxygen-derived free radicals and lipid peroxidation and tumor has close relationship.Superoxide dismutase (Superoxide Dismutase; SOD) be one of the scavenger of oxygen-derived free radicals active oxygen in the body; can eliminate oxygen-derived free radicals by catalysis and play the obstruction oxygen production, suppress the generation of lipid peroxide, the normal function of protection cell.(Malondialdehyde is the end product of the peroxide of lipid peroxidation generation MDA) to malonaldehyde, and its content usually reflects the body cell level of lipid peroxidation, and also the indirect reaction cell is subjected to the order of severity that free radical is attacked.
At present, great mass of data is verified: the ability decline that the genesis mechanism of each side difficult diseases such as inflammation, tumor, aging, hematopathy and the heart, liver, lung, skin and interior free yl produced too much or removed free radical has close relationship.Because it is antioxidant has interference effect to the body injury that is caused by free radical, and is acknowledged as a kind of new treatment approach, in the preventive medicine field, unprecedentedly surging to antioxidant research interest.Though synthetic antioxidant antioxygenic property is better, and certain side effect is arranged.So the natural drug of developmental research antioxidation is the most important thing of studying at present.
The present invention relates to a kind of compound recipe Fructus Mori preparation that can improve superoxide dismutase activity, for it provides reference frame in the new Natural antioxidant of exploitation of fields such as food, medicine.
Summary of the invention
Health food processing technique field under the present invention the present invention relates to a kind of compound recipe Fructus Mori preparation that can improve superoxide dismutase activity.Product of the present invention can be used for the new Natural antioxidants of field exploitation such as food, medicine, and product of the present invention is beneficial to suitability for industrialized production.
1, a kind of preparation method that can improve the compound recipe Fructus Mori preparation of superoxide dismutase activity is characterized in that may further comprise the steps:
(1) weight proportion of crude drug is: Fructus Mori 9g-12g, Fructus Lycii 6g-9g, Porphyridium cruentum 10g-20g;
(2) Fructus Mori and Fructus Lycii adding 8-10 are doubly measured lixiviate 8-10h under the 70% ethanol room temperature, seal ultrasonic 30min again, temperature is 30 ℃, power is 80W, uses filtered through gauze then, and filtering residue repeats to extract each once with the ethanol of 6 times of amounts and 4 times of amounts 70% respectively again, filter, get filtrate;
(3) filtrate in (2) is merged, reclaim ethanol, obtain Fructus Mori, Fructus Lycii extract;
(4) with the Porphyridium cruentum centrifugal 10min of 5000r/min centrifuge, be suspended in pH7.4 then, in the phosphate buffer of 50mmol/L, carry out ultrasonication, broken 5s, 5s 20 times, uses the centrifugal 15min of 10000r/min High speed refrigerated centrifuge at interval, get supernatant, lyophilization gets dry thing;
(5) with Fructus Mori, Fructus Lycii extract mix homogeneously in the dry thing in (4) and (3), get compound recipe Fructus Mori preparation.
2. compound recipe Fructus Mori preparation is tested, said preparation has the effect of the superoxide dismutase activity of raising.
According to the present invention, " % " among the present invention is percentage by weight.
The specific embodiment
Of the present inventionly relate to a kind of compound recipe Fructus Mori preparation that can improve superoxide dismutase activity and comprise following examples, the following examples can further specify the present invention, but do not limit the present invention in any way.
Embodiment 1:
1, a kind of preparation method that can improve the compound recipe Fructus Mori preparation of superoxide dismutase activity is characterized in that may further comprise the steps:
(1) weight proportion of crude drug is: Fructus Mori 9g, Fructus Lycii 6g, Porphyridium cruentum 10g;
(2) Fructus Mori and Fructus Lycii are added lixiviate 8 under 8 times of amount 70% ethanol room temperatures, seal ultrasonic 30min again, temperature is 30 ℃, power is 80W, uses filtered through gauze then, and filtering residue repeats to extract each once with the ethanol of 6 times of amounts and 4 times of amounts 70% respectively again, filter, get filtrate;
(3) filtrate in (2) is merged, reclaim ethanol, obtain Fructus Mori, Fructus Lycii extract;
(4) with the Porphyridium cruentum centrifugal 10min of 5000r/min centrifuge, be suspended in pH7.4 then, in the phosphate buffer of 50mmol/L, carry out ultrasonication, broken 5s, 5s 20 times, uses the centrifugal 15min of 10000r/min High speed refrigerated centrifuge at interval, get supernatant, lyophilization gets dry thing;
(5) with Fructus Mori, Fructus Lycii extract mix homogeneously in the dry thing in (4) and (3), get compound recipe Fructus Mori preparation.
2. compound recipe Fructus Mori preparation is tested, said preparation has the effect of the superoxide dismutase activity of raising.
Embodiment 2:
1, a kind of preparation method that can improve the compound recipe Fructus Mori preparation of superoxide dismutase activity is characterized in that may further comprise the steps:
(1) weight proportion of crude drug is: Fructus Mori 12g, Fructus Lycii 9g, Porphyridium cruentum 20g;
(2) Fructus Mori and Fructus Lycii are added lixiviate 10h under 10 times of amount 70% ethanol room temperatures, seal ultrasonic 30min again, temperature is 30 ℃, power is 80W, uses filtered through gauze then, and filtering residue repeats to extract each once with the ethanol of 6 times of amounts and 4 times of amounts 70% respectively again, filter, get filtrate;
(3) filtrate in (2) is merged, reclaim ethanol, obtain Fructus Mori, Fructus Lycii extract;
(4) with the Porphyridium cruentum centrifugal 10min of 5000r/min centrifuge, be suspended in pH7.4 then, in the phosphate buffer of 50mmol/L, carry out ultrasonication, broken 5s, 5s 20 times, uses the centrifugal 15min of 10000r/min High speed refrigerated centrifuge at interval, get supernatant, lyophilization gets dry thing;
(5) with Fructus Mori, Fructus Lycii extract mix homogeneously in the dry thing in (4) and (3), get compound recipe Fructus Mori preparation.
2. compound recipe Fructus Mori preparation is tested, said preparation has the effect of the superoxide dismutase activity of raising.
Pharmacological testing compound recipe Fructus Mori preparation improves the test of superoxide dismutase activity
1 material and animal
1.1 laboratory animal and feedstuff: healthy cleaning level Kunming mouse, Shandong Luye Pharmaceutical Co., Ltd. provides, body weight 18-22g, male and female half and half (SCXK (Shandong) 20030008).Animal feed: the big feed corporation,Ltd of Jinan health (Shandong is raised to give birth to examine and stepped on word (2005) No. 0474).
1.2 be subjected to the reagent thing:
Compound recipe Fructus Mori preparation.The weight proportion of crude drug is: Fructus Mori 12g, Fructus Lycii 9g, Porphyridium cruentum 20g.
Fructus Mori and Fructus Lycii are added lixiviate 10h under 10 times of amount 70% ethanol room temperatures, seal ultrasonic 30min again, temperature is 30 ℃, power is 80W, uses filtered through gauze then, and filtering residue repeats to extract each once with the ethanol of 6 times of amounts and 4 times of amounts 70% respectively again, filter, get filtrate; Filtrate is merged, reclaim ethanol, obtain Fructus Mori, Fructus Lycii extract; Porphyridium cruentum with the centrifugal 10min of 5000r/min centrifuge, is suspended in pH7.4 then, in the phosphate buffer of 50mmol/L, carries out ultrasonication, broken 5s, 5s 20 times, uses the centrifugal 15min of 10000r/min High speed refrigerated centrifuge at interval, get supernatant, lyophilization gets dry thing; With dry thing and Fructus Mori, Fructus Lycii extract mix homogeneously, get compound recipe Fructus Mori preparation, be the 100ml aqueous solution with compound recipe Fructus Mori preparation standardize solution.
SOD (superoxide dismutase) and MDA (malonaldehyde) test kit build up bio-engineering research by Nanjing and are produced, and lot number is 20090728.
1.3. experimental condition: ambient temperature: 22 ± 2 ℃, relative humidity 40-70%, animal is quarantined in feeding environment and adapts to 3d before the test.
1.4 experimental apparatus
SPN202F electronic balance (U.S. Ao Haosi), AR1140 electronic balance (U.S. Ao Haosi), 752 type ultraviolet-uisible spectrophotometers (Shanghai Spectrum Apparatus Co., Ltd.), SC-3610 low speed centrifuge (Keda Innovation Co., Ltd).
2 experimental techniques
Superoxide dismutase SOD and malonaldehyde MDA measure SOD and MDA measures according to the method for test kit.
3 experimental results
The results are shown in Table 1,2.
Table 1 male mice liver SOD, MDA value
Table 2 female mice liver SOD, MDA value
Be subjected to the reagent thing can obviously increase mouse liver SOD vigor, reduce MDA content, improve the activity of antioxidase, strengthen the ability of removing free radical, lipid peroxidation injury is reduced.
Claims (2)
1. preparation method that can improve the compound recipe Fructus Mori preparation of superoxide dismutase activity is characterized in that may further comprise the steps:
(1) weight proportion of crude drug is: Fructus Mori 9g-12g, Fructus Lycii 6g-9g, Porphyridium cruentum 10g-20g;
(2) Fructus Mori and Fructus Lycii adding 8-10 are doubly measured lixiviate 8-10h under the 70 weight % ethanol room temperatures, seal ultrasonic 30min again, temperature is 30 ℃, power is 80W, uses filtered through gauze then, and filtering residue repeats to extract each once with the ethanol of 6 times of amounts and 4 times of amount 70 weight % respectively again, filter, get filtrate;
(3) filtrate in (2) is merged, reclaim ethanol, obtain Fructus Mori, Fructus Lycii extract;
(4) with the Porphyridium cruentum centrifugal 10min of 5000r/min centrifuge, be suspended in pH7.4 then, in the phosphate buffer of 50mmol/L, carry out ultrasonication, broken 5s, 5s 20 times, uses the centrifugal 15min of 10000r/min High speed refrigerated centrifuge at interval, get supernatant, lyophilization gets dry thing;
(5) with Fructus Mori, Fructus Lycii extract mix homogeneously in the dry thing in (4) and (3), get compound recipe Fructus Mori preparation.
2. the purposes of a kind of compound recipe Fructus Mori preparation described in claim 1 is characterized in that preparing the application that can improve in the superoxide dismutase activity medicine.
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| CN104432043A (en) * | 2014-11-29 | 2015-03-25 | 黄南概 | Green alga and mulberry health buccal tablets and preparation method thereof |
| CN104432094A (en) * | 2014-11-29 | 2015-03-25 | 黄南概 | Spirulina-mulberry health buccal tablets and preparation method thereof |
| CN104432093A (en) * | 2014-11-29 | 2015-03-25 | 黄南概 | Microalgae mulberry healthcare buccal tablet and preparation method thereof |
| CN106858592A (en) * | 2017-03-07 | 2017-06-20 | 青海极域生物资源开发有限公司 | Lycium ruthenicum pure powder tablet preparation method |
| CN107927784B (en) * | 2017-12-15 | 2021-02-05 | 齐鲁工业大学 | Optimization research of paste preparation process of mulberry extraction pulp |
| CN110150521A (en) * | 2019-05-15 | 2019-08-23 | 山东圣源绿色食品科技有限公司 | A kind of anti-oxidation health beverage of kidney tonifying protect liver |
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| CN101449821A (en) * | 2007-11-30 | 2009-06-10 | 北京因科瑞斯医药科技有限公司 | Composition capable of releasing asthenopia and preparation method thereof |
| CN101683161A (en) * | 2008-09-26 | 2010-03-31 | 刘泳宏 | Natural tea drink capable of clearing away heat and production method |
| CN102188658A (en) * | 2011-04-21 | 2011-09-21 | 北京身心康国际中医研究院 | Chinese medicine preparation for treating essence and blood deficiency for kidney-yin deficiency patients, preparation method thereof and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101449821A (en) * | 2007-11-30 | 2009-06-10 | 北京因科瑞斯医药科技有限公司 | Composition capable of releasing asthenopia and preparation method thereof |
| CN101683161A (en) * | 2008-09-26 | 2010-03-31 | 刘泳宏 | Natural tea drink capable of clearing away heat and production method |
| CN102188658A (en) * | 2011-04-21 | 2011-09-21 | 北京身心康国际中医研究院 | Chinese medicine preparation for treating essence and blood deficiency for kidney-yin deficiency patients, preparation method thereof and application thereof |
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