CN102507522B - Data acquisition method and heating system for oxygen radical absorbance capacity measuring analyzer - Google Patents
Data acquisition method and heating system for oxygen radical absorbance capacity measuring analyzer Download PDFInfo
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- CN102507522B CN102507522B CN201110346694.0A CN201110346694A CN102507522B CN 102507522 B CN102507522 B CN 102507522B CN 201110346694 A CN201110346694 A CN 201110346694A CN 102507522 B CN102507522 B CN 102507522B
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Abstract
The invention relates to an antioxidant capacity analyzing tester in the field of analyzing testers, in particular to a data acquisition method and a heating system for an oxygen radical absorbance capacity (ORAC) measuring analyzer. A method for acquiring data by scanning is adopted on a data acquisition system with a positive temperature coefficient (PTC) constant temperature heating system, namely in a measured tube diameter range of a certain data acquisition point, data sets are acquired by scanning in a short period of time, and the maximum data acquired by scanning in the short period of time is selected as data acquired at the time point. By the invention, the coefficient of variation of the data sets acquired by the type of instruments can be obviously reduced on statistical significance, so that errors of a measurement result within assays and a measurement result between assays are directly reduced obviously.
Description
Technical field
The oxidation resistance that the present invention relates to analyze in determining instrument field is analyzed collecting method and heating system in analyzer, particularly a kind of oxygen radical receptivity (ORAC) determination and analysis instrument.
Background technology
The data acquisition in determination and analysis of existing oxygen radical receptivity (ORAC) determination and analysis instrument adopts " some collecting method ", this fixed point acquisition method is that the several sample hoses to be determined on tubular type specimen holder are moved on to detection position successively, by pipe, measure fluorescence data value (see figure 1), its heating system adopts the electrical heating of instrument cavity.
Due to this quasi-instrument in determination and analysis process, using gather each time point fluorescence emission strong and weak data as determination data, in certain hour section (30 minutes-60 minutes), the data point set constituent curve gathering, as a certain reagent at time dependent curve of this time period, by several agent combination, the data point set that gathers these several reagent in certain hour section forms several curves, and by calculating, can obtain oxygen radical receptivity (ORAC) measurement result of sample to be determined, therefore when some time point image data, within the scope of tested fixed tube interior diameter, the concave moon surface of fluid to be measured liquid level makes measured point liquid level have height, image data position and a little skew of measuring light path center, many factors such as liquid level that tested fixed tube is in operation is unstable, capital causes in this time point left and right of image data (5-10 seconds) institute image data in a bit of time to have certain difference, and put a certain position sometime, adopt to obtain fluorescent emission data, directly affect the correctness of institute's image data.In other words, the data of (< 0.9cm) difference collection have larger difference in the cursor in-scope within the scope of tested fixed tube interior diameter.Prior art image data is all with " some collecting method ", and as shown in Figure 2, specimen holder is moved to the left to the disposable fixed point image data in exciting light focal position from initial position the central point of the 1st sample hose to its collecting flowchart.Then same method, then according to gathering 2～8 pipe data.Be the light initial point of emission wavelength in which which position of time, just at this time point, gather this position data.From collection effect, as shown in Figure 3: when fixed point gathers, due to a variety of causes, collection point is not necessarily at the peak of curve.And may occur (each circulation) each time data acquisition of same tested fixed tube not necessarily completely at same identical position and the identical time point of this mensuration pipe, the difference slightly of each data like this, will cause the coefficient of variation of institute's image data collection to increase, therefore United States Department of Agriculture (authority of this quasi-instrument and founder) stipulates, as ORAC, analyze the reliable data of measurement result, its coefficient of variation is≤15%.
In addition, because existing ORAC analyser is the electrical heating of instrument cavity, therefore the temperature of each tested fixed tube can be strictly not identical, respectively manage fluid to be measured temperature contrast and be difficult to reach the strict same range as of 0.05 ℃ of <, and temperature there is considerable influence to ORAC determination data result exactly.
Summary of the invention
The object of the present invention is to provide collecting method and heating system in a kind of oxygen radical receptivity (ORAC) determination and analysis instrument, mainly solve the existing problem of above-mentioned prior art, implement the present invention to can significantly reducing the coefficient of variation of this quasi-instrument institute image data collection in statistical significance, thus in directly significantly reducing batch and batch between the error of measurement result.
For achieving the above object, the present invention realizes like this.
Collecting method in a kind of oxygen radical receptivity determination and analysis instrument, it is characterized in that: it has the upper method that adopts scanning collection data of the data acquisition system (DAS) of PTC constant-temperature heating system (as Fig. 1), in the mensuration pipe diameter range of certain data collection point, at a bit of time interscan image data collection, select this segment scan maximum data, the data that gather as this time point.
Collecting method in described oxygen radical receptivity determination and analysis instrument, it is characterized in that: specifically move on to every front 30 steps in sample hose center and start scanning until 30 steps after every tube hub, every step all will be tested and be gathered a secondary data, by the maximal value in 60 secondary data as acquired data values, as shown in Figure 4,5.From effect, when scanning fixed point gathers, whatsoever factor is disturbed, and is all to find peak in 60 collection points.
The heating system of using in collecting method in above-mentioned oxygen radical receptivity determination and analysis instrument, as shown in Figure 6,7: it comprises that a specimen holder supporting case 11 and lid are located at the specimen holder sheet metal 12 on specimen holder supporting case 11, these specimen holder sheet metal 12 back sides are fixed with respectively PTC heating plate 14 and temperature sensor 15 by heat-conducting glue 13, and these specimen holder sheet metal 12 fronts have for putting the working sample frame groove 16 of specimen holder.Described specimen holder adopts identical metal material with specimen holder sheet metal 12.
Concrete, well heater adopts the PTC electronic ceramics of power 100w, and temperature sensor Pt100, in order to improve control accuracy, adopts the Pt100 bridge diagram of constant current, and instrument amplification and reference power supply, as 37 ℃ of given voltages, are controlled sensitivity to improve temperature; PTC drive current changes within the scope of 220V ~ 20MA～500MA, so the temperature accuracy of specimen holder is higher.Its temperature control principle drawing as shown in Figure 8.
Because original heating system adopts the electrical heating of instrument cavity, need to make sample chamber environment keep constant temperature, but this constant temperature method not only can not be strict constant temperature require (respectively managing 0.05 ℃ of fluid to be measured temperature contrast <), and this temperature control often has hysteresis quality, general constant temperature precision is at 0.5 ℃～1.0 ℃, and temperature contrast has a significant impact ORAC measurement result.And heating system of the present invention adopts PTC temperature control, PTC heating plate and specimen holder are arranged on one, whole close contact, and PTC heating plate jointly moves together with specimen holder under the drive of shift servo mechanism, replaces common cavity electrical heating constant temperature system.
Accompanying drawing explanation
Fig. 1 be oxygen radical receptivity (ORAC) determination and analysis instrument by pipe data acquisition system (DAS) structural representation.
Fig. 2 is existing fixed point collecting flowchart figure.
Fig. 3 is existing fixed point collection effect figure.
Fig. 4 is the process flow diagram of the inventive method.
Fig. 5 is the inventive method scan-data collection effect figure.
Fig. 6 is the structural representation of heating system of the present invention.
Fig. 7 is the structural representation at the specimen holder sheet metal back side of heating system of the present invention.
Fig. 8 is the PTC temperature control principle of heating system of the present invention.
Embodiment
One, sample tube frame temperature stability is investigated result
Method: two thermometers are placed respectively and the nearly two ends of working sample frame groove (Fig. 6), and with rooved face close contact.Specimen holder groove is under stepper motor drives simultaneously, and during by mensuration, the same manner moves around, and in certain hour section, regularly reads specimen holder rooved face temperature value.The results are shown in following table 1.
Table 1: the temperature control effect of specimen holder sheet metal (sample hose internal solvent temperature is in design temperature (37) ± 0.1 ℃):
From table, in the minute of two hours (it is 0.5～1 hour that general ORAC analyzes minute), two ends temperature variation is all very little, and extreme difference is respectively 0.05 ℃ and 0.04 ℃.The coefficient of variation is respectively 0.015136% and 0.018684%.Two ends temperature difference is 0.03 ℃ to the maximum.All far below design error ± 0.1 ℃
The present invention relates to the change of collecting method and heating element in a kind of oxygen radical receptivity (ORAC) determination and analysis instrument, its goal of the invention is to reduce oxygen radical receptivity (ORAC) the analyzer coefficient of variation of institute's image data under the same conditions, and temperature is reduced to Min. to the impact of measurement result, will directly reduce the error of measurement result.
Two, two kinds of collecting methods are measured the comparison of same sample fluorescent value result
Method: by certain density synthetic fluorescein Sodum Fluorescence(3 ', 6 '-dihydroxyspiro[isobenzofuran-1[3H], 9 ' [9H]-xanthen]-3-one, be called for short FL) be placed in respectively the test tube (have 8 and measure pipe) on each mensuration pipe support, with fixed-point data acquisition method and scan-data acquisition method, measure respectively with excitation wavelength: 485nm; Wavelength of transmitted light: 538nm measure one by one 8 pipes fluorescent value data, every pipe is measured 10 times, investigates respectively the stability of two kinds of acquisition methods, the results are shown in Table scan-data acquisition method in 2(table is " method 1 "; Fixed-point data acquisition method is " method 2 ")
Table 2:8 manages every pipe and measures 10 fluorescent emission light values
By table 2, by the 10 secondary data coefficient of variation (CV%) that the every pipe of scan-data acquisition method (method 1) is measured, be 0.43% to the maximum as seen, and being 16.87% to the maximum by the coefficient of variation that the every pipe of fixed-point data acquisition method (method 2) is measured 10 secondary data, both coefficient of variation differ nearly 40 times.Visual scan data acquisition method is extremely significantly better than fixed-point data acquisition method.
Table 3 is respectively managed the significance of difference statistics between average
From table 3, from 8 population means of measuring pipes, fix point method is low nearlyer 21% than scanning method, and what visible fix point method measurement result embodied is not exclusively the True Data value of tested reagent.The coefficient of variation between 8 pipes differs from 16.3 times, and the stability approach 1 of visible two kinds of method measurement results is significantly better than method 2.Through TTEST check, two kinds of collecting method measurement result otherness P=3.01 * 10-6 < 0.01, show that the data that two kinds of collecting methods are measured have utmost point significant difference.
Claims (4)
1. collecting method in an oxygen radical receptivity determination and analysis instrument, it is characterized in that: it adopts the method for scanning collection data in the data acquisition system (DAS) with PTC constant-temperature heating system, in the mensuration pipe diameter range of certain data collection point, at a bit of time interscan image data collection, select this segment scan maximum data, the data that gather as this time point.
2. collecting method in oxygen radical receptivity determination and analysis instrument according to claim 1, it is characterized in that: specifically move on to every front 30 steps in sample hose center and start scanning until 30 steps after every tube hub, every step all will be tested and be gathered a secondary data, by the maximal value in 60 secondary data as acquired data values.
3. the heating system of using in collecting method in an oxygen radical receptivity determination and analysis instrument as claimed in claim 1 or 2, it is characterized in that: it comprises that a specimen holder supporting case (11) and lid are located at the specimen holder sheet metal (12) on specimen holder supporting case (11), this specimen holder sheet metal (12) back side is fixed with respectively PTC heating plate (14) and temperature sensor (15) by heat-conducting glue (13), and this specimen holder sheet metal (12) is positive to be had for putting the working sample frame groove (16) of specimen holder.
4. heating system according to claim 3, is characterized in that: described specimen holder and specimen holder sheet metal (12) adopt identical metal material.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4667105A (en) * | 1984-06-09 | 1987-05-19 | Horiba, Ltd. | Infrared radiation gas analyzer with PTC resistance heater |
| CN1594830A (en) * | 2004-06-17 | 2005-03-16 | 沈阳金田石油机械制造有限公司 | Intelligent oil recovery control system and control method thereof |
| JP2007504443A (en) * | 2003-08-26 | 2007-03-01 | マナテック、インク． | Antioxidant sensor, detection method, and composition |
| CN201926624U (en) * | 2010-12-03 | 2011-08-10 | 上海智城分析仪器制造有限公司 | Light source system in oxygen radical absorbing capacity determination analyzer |
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| JPS62151760A (en) * | 1985-12-26 | 1987-07-06 | Toshiba Corp | Liquid heating unit for sample inspection system |
| CN101359041A (en) * | 2008-09-27 | 2009-02-04 | 上海微电子装备有限公司 | Synchronous calibration method and system for intensity sampling of pulse |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4667105A (en) * | 1984-06-09 | 1987-05-19 | Horiba, Ltd. | Infrared radiation gas analyzer with PTC resistance heater |
| JP2007504443A (en) * | 2003-08-26 | 2007-03-01 | マナテック、インク． | Antioxidant sensor, detection method, and composition |
| CN1594830A (en) * | 2004-06-17 | 2005-03-16 | 沈阳金田石油机械制造有限公司 | Intelligent oil recovery control system and control method thereof |
| CN201926624U (en) * | 2010-12-03 | 2011-08-10 | 上海智城分析仪器制造有限公司 | Light source system in oxygen radical absorbing capacity determination analyzer |
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