CN102507522A - Data acquisition method and heating system for oxygen radical absorbance capacity measuring analyzer - Google Patents
Data acquisition method and heating system for oxygen radical absorbance capacity measuring analyzer Download PDFInfo
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- CN102507522A CN102507522A CN2011103466940A CN201110346694A CN102507522A CN 102507522 A CN102507522 A CN 102507522A CN 2011103466940 A CN2011103466940 A CN 2011103466940A CN 201110346694 A CN201110346694 A CN 201110346694A CN 102507522 A CN102507522 A CN 102507522A
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Abstract
The invention relates to an antioxidant capacity analyzing tester in the field of analyzing testers, in particular to a data acquisition method and a heating system for an oxygen radical absorbance capacity (ORAC) measuring analyzer. A method for acquiring data by scanning is adopted on a data acquisition system with a positive temperature coefficient (PTC) constant temperature heating system, namely in a measured tube diameter range of a certain data acquisition point, data sets are acquired by scanning in a short period of time, and the maximum data acquired by scanning in the short period of time is selected as data acquired at the time point. By the invention, the coefficient of variation of the data sets acquired by the type of instruments can be obviously reduced on statistical significance, so that errors of a measurement result within assays and a measurement result between assays are directly reduced obviously.
Description
Technical field
The present invention relates to the oxidation resistance assay determination appearance in the assay determination instrument field, collecting method and heating system in particularly a kind of oxygen radical receptivity (ORAC) determination and analysis appearance.
Background technology
" point data acquisition method " adopted in the data acquisition in determination and analysis of existing oxygen radical receptivity (ORAC) determination and analysis appearance; This fixed point acquisition method is that the several sample hoses to be determined on the tubular type specimen holder are moved on to the detection position successively; Measure fluorescence data value (see figure 1) by pipe, its heating system then adopts the electrical heating of instrument cavity.
Since this quasi-instrument in the determination and analysis process with the data of the power of gathering each time point fluorescence emission as determination data, in certain hour section (30 minutes-60 minutes), the data point set of being gathered is formed curve; As a certain reagent at time dependent curve of this time period; Through several kinds of agent combination, gather the data point set of these several kinds of reagent in the certain hour section and form several curves, and through calculating; The oxygen radical receptivity (ORAC) that can obtain sample to be determined is measured the result; Therefore when some time point image data, in tested fixed tube interior diameter scope, the concave moon surface of fluid to be measured liquid level makes the measured point liquid level that height arranged; Image data position and a little skew of measuring the light path center; Many factors such as the liquid level instability that tested fixed tube is in operation; The capital causes in this time point left and right sides of image data that (5-10 seconds) institute image data has certain difference in a bit of time; And put sometime a certain position adopt fluorescent emission data, directly influence the correctness of institute's image data.In other words, promptly in the cursor in-scope in tested fixed tube interior diameter scope (＜0.9cm) data of difference collection have than big-difference.The prior art image data all is that its collecting flowchart is as shown in Figure 2 with " point data acquisition method ", and specimen holder is moved to the left the disposable fixed point image data in exciting light focal position to the central point of the 1st sample hose from initial position.Same then quadrat method is again according to gathering 2～8 pipe data.The light initial point that is emission wavelength is just gathered this position data at this time point in which which position of time.See that from collection effect as shown in Figure 3: when fixed point was gathered, because a variety of causes, the collection point was not necessarily at the peak of curve.And (each circulation) each time data acquisition not necessarily complete same identical position and identical time point at this mensuration pipe to same tested fixed tube possibly appear; The difference slightly of each data like this; Will cause the coefficient of variation of institute's image data collection to increase; Therefore United States Department of Agriculture (authority of this quasi-instrument and founder) stipulates, as the data of ORAC assay determination reliable results, its coefficient of variation is≤15%.
In addition; Because existing ORAC analyser is the electrical heating of instrument cavity; Therefore the temperature of each tested fixed tube can be strict not identical, promptly respectively manage the fluid to be measured temperature contrast and be difficult to reach＜0.05 ℃ strict same range as, and temperature has considerable influence to ORAC determination data result exactly.
Summary of the invention
The object of the present invention is to provide collecting method and heating system in a kind of oxygen radical receptivity (ORAC) determination and analysis appearance; Mainly solve above-mentioned existing in prior technology problem; Embodiment of the present invention be to can significantly reducing the coefficient of variation of this quasi-instrument institute image data collection on statistical significance, thus in directly significantly reducing batch and batch between measure result's error.
For realizing above-mentioned purpose, the present invention realizes like this.
Collecting method in a kind of oxygen radical receptivity determination and analysis appearance; It is characterized in that: it goes up the method that adopts the scanning collection data in the data acquisition system (DAS) with PTC heated at constant temperature system (like Fig. 1); Promptly in the mensuration pipe diameter range of certain data collection point; At a bit of time interscan image data collection, select this segment scan maximum data, the data of gathering as this time point.
Collecting method in the described oxygen radical receptivity determination and analysis appearance; It is characterized in that: specifically be to move on to preceding 30 steps of every sample hose center to begin scanning till 30 steps behind every tube hub; Per step all will be tested and gathered a secondary data; With the maximal value in 60 secondary data as acquired data values, like Fig. 4, shown in 5.See that from effect when the scanning fixed point was gathered, whatsoever factor was disturbed, and all is in 60 collection points, to find peak.
The heating system of using in the collecting method in the above-mentioned oxygen radical receptivity determination and analysis appearance; Like Fig. 6, shown in 7: it comprises that a specimen holder supporting case 11 and lid are located at the specimen holder sheet metal 12 on the specimen holder supporting case 11; These specimen holder sheet metal 12 back sides are fixed with PTC heating plate 14 and temperature sensor 15 respectively through heat-conducting glue 13, and these specimen holder sheet metal 12 fronts then have the working sample frame groove 16 that is used to put specimen holder.Described specimen holder adopts identical metal material with specimen holder sheet metal 12.
Concrete, well heater adopts the PTC electronic ceramics of power 100w, and temperature sensor Pt100 in order to improve control accuracy, adopts the Pt100 bridge diagram of constant current, and instrument amplification and reference power supply are as 37 ℃ of given voltages, to improve temperature control sensitivity; The PTC drive current changes in 220V ~ 20MA～500MA scope, so the temperature accuracy of specimen holder is higher.Figure is as shown in Figure 8 for its temperature control principle.
Because original heating system adopts the electrical heating of instrument cavity; Need make the sample chamber environment keep constant temperature; But this constant temperature method not only can not be strict constant temperature require (respectively manage fluid to be measured temperature contrast＜0.05 ℃); And this temperature control often has hysteresis quality, and general constant temperature precision is at 0.5 ℃～1.0 ℃, and temperature contrast has very big influence to ORAC mensuration result.And heating system of the present invention adopts the PTC temperature control, and PTC heating plate and specimen holder are installed in one, and integral body contacts closely, and the PTC heating plate replaces common cavity electrical heating constant temperature system with specimen holder common moving under shift servo mechanism drives.
Description of drawings
Fig. 1 is that pursuing of oxygen radical receptivity (ORAC) determination and analysis appearance managed the data acquisition system (DAS) structural representation.
Fig. 2 is existing fixed point collecting flowchart figure.
Fig. 3 is existing fixed point collection effect figure.
Fig. 4 is the process flow diagram of the inventive method.
Fig. 5 is the inventive method scan-data collection effect figure.
Fig. 6 is the structural representation of heating system of the present invention.
Fig. 7 is the structural representation at the specimen holder sheet metal back side of heating system of the present invention.
Fig. 8 is the PTC temperature control principle of heating system of the present invention.
Embodiment
One, sample tube frame temperature stability is investigated the result
Method: two thermometers are placed respectively and the nearly two ends of working sample frame groove (Fig. 6), and closely contacted with rooved face.The specimen holder groove is under step motor drive simultaneously, and the same manner moves around during by mensuration, in the certain hour section, regularly reads specimen holder rooved face temperature value.The result sees the following form 1.
Table 1: the temperature control effect of specimen holder sheet metal (sample hose internal solvent temperature design temperature (37) ± 0.1 ℃):
Visible by table, in two hours minute (the general ORAC assay determination time is 0.5～1 hour), the two ends temperature variation is all very little, and extreme difference is respectively 0.05 ℃ and 0.04 ℃.The coefficient of variation is respectively 0.015136% and 0.018684%.The two ends temperature difference is 0.03 ℃ to the maximum.All far below design error ± 0.1 ℃
The present invention relates to the change of collecting method and heating element in a kind of oxygen radical receptivity (ORAC) determination and analysis appearance; Its goal of the invention is to reduce oxygen radical receptivity (ORAC) the analyzer coefficient of variation of institute's image data under the same conditions; And temperature is reduced to Min. to the influence of measuring the result, with directly reducing the error of measuring the result.
Two, two kinds of collecting methods are measured same sample fluorescence value result relatively
Method: with certain density synthetic luciferin Sodum Fluorescence (3 '; 6 '-dihydroxyspiro [isobenzofuran-1 [3H]; 9 ' [9H]-xanthen]-3-one; Be called for short FL) place each to measure the test tube (have 8 and measure pipe) on the pipe support respectively, measure respectively with excitation wavelength with fixed-point data acquisition method and scan-data acquisition method: 485nm; Wavelength of transmitted light: 538nm measure one by one 8 pipes the fluorescent value data, every pipe is measured 10 times, investigates the stability of two kinds of acquisition methods respectively, the result sees that (the scan-data acquisition method is " method 1 " to table 2 in the table; The fixed-point data acquisition method is " method 2 ")
Table 2:8 manages every pipe and measures 10 fluorescent emission light values
Be 0.43% to the maximum by the visible 10 secondary data coefficient of variation of measuring with the every pipe of scan-data acquisition method (method 1) (CV%) of table 2; And being 16.87% to the maximum with the coefficient of variation that the every pipe of fixed-point data acquisition method (method 2) is measured 10 secondary data, both coefficient of variation differ nearly 40 times.Visual scan data acquisition method significantly is superior to the fixed-point data acquisition method unusually.
Table 3 is respectively managed the significance of difference statistics between the average
Visible by table 3, see that from 8 population means of measuring pipe fix point method is low nearlyer 21% than scanning method, what visible fix point method was measured result's embodiment not exclusively is the True Data value of tested reagent.The coefficient of variation between 8 pipes differs from 16.3 times, and the stability approach 1 that visible two kinds of methods are measured the result significantly is superior to method 2.Through the TTEST check, two kinds of collecting methods are measured result difference property P=3.01 * 10-6＜0.01, show that the data that two kinds of collecting methods are measured have utmost point significant difference.
Claims (4)
1. collecting method in the oxygen radical receptivity determination and analysis appearance; It is characterized in that: it adopts the method for scanning collection data on the data acquisition system (DAS) with PTC heated at constant temperature system; Promptly in the mensuration pipe diameter range of certain data collection point; At a bit of time interscan image data collection, select this segment scan maximum data, the data of gathering as this time point.
2. collecting method in the oxygen radical receptivity determination and analysis appearance according to claim 1; It is characterized in that: specifically be to move on to preceding 30 steps of every sample hose center to begin scanning till 30 steps behind every tube hub; Per step all will be tested and gathered a secondary data, with the maximal value in 60 secondary data as acquired data values.
3. the heating system of using in the collecting method in the oxygen radical receptivity determination and analysis appearance according to claim 1 or claim 2; It is characterized in that: it comprises that a specimen holder supporting case (11) and lid are located at the specimen holder sheet metal (12) on the specimen holder supporting case (11); This specimen holder sheet metal (12) back side is fixed with PTC heating plate (14) and temperature sensor (15) respectively through heat-conducting glue (13), and this specimen holder sheet metal (12) front then has the working sample frame groove (16) that is used to put specimen holder.
4. heating system according to claim 3 is characterized in that: described specimen holder adopts identical metal material with specimen holder sheet metal (12).
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104807797A (en) * | 2015-05-12 | 2015-07-29 | 扬州市扬大康源乳业有限公司 | Method for measuring antioxidant activity of lactic acid bacteria based on cellular level |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4667105A (en) * | 1984-06-09 | 1987-05-19 | Horiba, Ltd. | Infrared radiation gas analyzer with PTC resistance heater |
| JPS62151760A (en) * | 1985-12-26 | 1987-07-06 | Toshiba Corp | Liquid heating unit for sample inspection system |
| CN1594830A (en) * | 2004-06-17 | 2005-03-16 | 沈阳金田石油机械制造有限公司 | Intelligent oil recovery control system and control method thereof |
| JP2007504443A (en) * | 2003-08-26 | 2007-03-01 | マナテック、インク． | Antioxidant sensor, detection method, and composition |
| CN101359041A (en) * | 2008-09-27 | 2009-02-04 | 上海微电子装备有限公司 | Synchronous calibration method and system for intensity sampling of pulse |
| CN201926624U (en) * | 2010-12-03 | 2011-08-10 | 上海智城分析仪器制造有限公司 | Light source system in oxygen radical absorbing capacity determination analyzer |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4667105A (en) * | 1984-06-09 | 1987-05-19 | Horiba, Ltd. | Infrared radiation gas analyzer with PTC resistance heater |
| JPS62151760A (en) * | 1985-12-26 | 1987-07-06 | Toshiba Corp | Liquid heating unit for sample inspection system |
| JP2007504443A (en) * | 2003-08-26 | 2007-03-01 | マナテック、インク． | Antioxidant sensor, detection method, and composition |
| CN1594830A (en) * | 2004-06-17 | 2005-03-16 | 沈阳金田石油机械制造有限公司 | Intelligent oil recovery control system and control method thereof |
| CN101359041A (en) * | 2008-09-27 | 2009-02-04 | 上海微电子装备有限公司 | Synchronous calibration method and system for intensity sampling of pulse |
| CN201926624U (en) * | 2010-12-03 | 2011-08-10 | 上海智城分析仪器制造有限公司 | Light source system in oxygen radical absorbing capacity determination analyzer |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104807797A (en) * | 2015-05-12 | 2015-07-29 | 扬州市扬大康源乳业有限公司 | Method for measuring antioxidant activity of lactic acid bacteria based on cellular level |
| CN104807797B (en) * | 2015-05-12 | 2018-04-06 | 扬州市扬大康源乳业有限公司 | A kind of method based on cellular level measure lactic acid bacteria antioxidation activity |
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