CN111189800A - Method for determining content and concentration of vitronectin by using refractive index test - Google Patents
Method for determining content and concentration of vitronectin by using refractive index test Download PDFInfo
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- CN111189800A CN111189800A CN201911396367.9A CN201911396367A CN111189800A CN 111189800 A CN111189800 A CN 111189800A CN 201911396367 A CN201911396367 A CN 201911396367A CN 111189800 A CN111189800 A CN 111189800A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
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Abstract
The invention belongs to the field of fine chemicals and daily chemicals, and particularly relates to a method for determining content and concentration of vitronectin by using a refractive index test. The method comprises the steps of measuring the refractive indexes of a series of vitreous color factor solutions with different concentrations by utilizing the characteristic that the refractive index of the vitreous color factor solution is in a linear relation with the concentration of the vitreous color factor solution, drawing a linear graph of the relation between the refractive indexes and the concentration, obtaining a functional relation between the concentration and the refractive index, finally measuring the refractive index of the vitreous color factor solution with unknown concentration, and calculating the concentration of the vitreous color factor based on the functional relation between the concentration and the refractive index. The method is simple and convenient to operate, simple in instrument and short in testing time, and is suitable for accurate and rapid determination in the fields of cosmetics, fine chemical engineering and the like.
Description
Technical Field
The invention belongs to the field of fine chemicals and daily chemicals, and particularly relates to a method for determining content and concentration of vitronectin by using a refractive index test.
Background
Vitriol, also known as hydroxypropyl tetrahydropyrane triol (CAS number 439685-79-7), is a bioactive substance, can resist skin aging, dehydration and other symptoms, and is widely applied to various fields of food, biology, medicines, cosmetics and the like. To achieve these applications, the content of the vitreous color factor must first be accurately determined. Therefore, how to quickly and effectively determine the actual content of the vitreous color in various formulas has important significance for the production and detection of related products. For quantitative analysis of organic compounds, Liquid Chromatography (LC), Gas Chromatography (GC), gas chromatography-mass spectrometry (GC-MS), fluorescence, ultraviolet-visible spectrophotometer, and the like are commonly used. These methods often require complex instrumentation, sample pre-treatment, or long test times. Therefore, it is highly desirable to develop a method for determining the concentration of the content of the vitronectin with high efficiency and accuracy.
Disclosure of Invention
The invention aims to provide a method for determining the content and concentration of a vitreous color factor by using a refractive index test.
In order to achieve the purpose of the invention, the invention adopts the technical scheme that: a method for determining the content and concentration of vitronectin by using a refractive index test comprises the following steps:
(1) preparing an instrument: preparing an automatic Abbe refractometer WYT-ZT, fully cleaning the glass surface of a test dish by using high-grade mirror wiping paper, and covering the glass surface with a cover. If the surface is stained with oil dirt, the surface can be wiped clean by using alcohol.
(2) Preparing a sample: preparing the vitronectin solutions with the concentrations of 10%, 20%, 30%, 40% and 50% respectively.
(3) The refractive index of the solution with the concentration at 20 ℃ is measured by an Abbe refractometer.
(4) And (4) drawing a linear graph of the relationship between the refractive index and the concentration according to the data in the step (3), and obtaining a functional relationship between the concentration and the refractive index.
(5) And (4) taking one part of the vitronectin aqueous solution, determining the refractive index of the aqueous solution, and calculating the content of the vitronectin according to the functional relation obtained in the step (4).
The invention has the beneficial effects that:
the invention provides a method for determining content and concentration of a vitreous color factor by using a refractive index test. The method is simple and convenient to operate, simple in instrument and short in testing time, and is suitable for accurate and rapid determination in the fields of cosmetics, fine chemical engineering and the like.
Drawings
FIG. 1 is a linear plot of refractive index versus concentration as referred to in example one.
Detailed Description
Example one (determination of refractive index of a standard and calculation of the linearity of the refractive index versus concentration):
(1) preparing an automatic Abbe refractometer WYT-ZT, fully cleaning the glass surface of a test dish by using high-grade mirror wiping paper, and covering the glass surface with a cover. If the surface is stained with oil dirt, the surface can be wiped clean by using alcohol.
(2) The method is characterized in that the vitreous color factor solutions with different concentrations are prepared from the following components in percentage by mass:
50 percent of butanediol
0 to 50 percent of vitreous origin
Pure water balance
(3) The refractive index of the solution with the concentration at 20 ℃ is measured by an Abbe refractometer:
and clicking a test monitoring button to switch the temperature control switch to an ON state to start controlling the temperature of the sample cell. And after the sample pool reaches the set temperature, opening the cover, dripping liquid to be measured, covering the cover when the sample amount reaches the depth of more than 3-5 mm, and waiting for 1-3 minutes to balance the liquid temperature and the control temperature of the instrument. Selecting the type of measurement required: refracting light (n)D) And a soluble solid. The measurement is started by clicking the "test" button and after the measurement is finished, the reading is taken and recorded (taking the average of the two readings as the refractive index/soluble solids of the sample). The sample cell was wiped clean and covered with a lid.
Repeating the steps, respectively measuring the refractive indexes of the vitronectin aqueous solutions with different concentrations, and obtaining the following results:
| concentration of | 10％ | 20％ | 30％ | 40％ | 50％ |
| Refractive index | 1.407 | 1.422 | 1.438 | 1.451 | 1.465 |
(4) And (4) obtaining the linear relation between the refractive index and the concentration according to the data in the step (3) as shown in figure 1, wherein the function relation is as follows:
y is 0.145x +1.3931, where y is the refractive index and x is the concentration of the vitronectin.
Example two (determination of unknown sample concentration):
a portion of the vitronectin solution was taken, the vitronectin content was unknown, the refractive index was determined to be 1.442, and the vitronectin content was calculated to be 33.7% based on the functional relationship obtained in the first example.
Example three (determination of unknown sample concentration):
a portion of the vitronectin solution was taken, the vitronectin content was unknown, the refractive index was determined to be 1.432, and the vitronectin content was calculated to be 26.8% based on the functional relationship obtained in example one.
Example four (determination of unknown sample concentration):
a portion of the vitronectin solution was taken, the vitronectin content was unknown, the refractive index was determined to be 1.412, and the vitronectin content was calculated to be 13.0% based on the functional relationship obtained in the first example.
Claims (6)
1. A method for determining the content and concentration of vitronectin by using a refractive index test is characterized by comprising the following steps: the method comprises the steps of measuring the refractive indexes of a series of vitreous color factor solutions with different concentrations by utilizing the characteristic that the refractive index of the vitreous color factor solution is in a linear relation with the concentration of the vitreous color factor solution, drawing a linear graph of the relation between the refractive indexes and the concentration, obtaining a functional relation between the concentration and the refractive index, finally measuring the refractive index of the vitreous color factor solution with unknown concentration, and calculating the concentration of the vitreous color factor based on the functional relation between the concentration and the refractive index.
2. The method for determining concentration of vitronectin content by using refractive index test as claimed in claim 1, which comprises the following steps:
(1) preparing an automatic Abbe refractometer, fully cleaning the glass surface of a test dish by using high-grade lens wiping paper, and covering the glass surface with a cover.
(2) Preparing a sample: the method is characterized in that the vitreous color factor solutions with different concentrations are prepared from the following components in percentage by mass:
50 percent of butanediol
0 to 50 percent of vitreous origin
Pure water balance
(3) The refractive index of the solution with the above concentration is measured by an Abbe refractometer.
(4) And (4) drawing a linear graph of the relationship between the refractive index and the concentration according to the data in the step (3), and obtaining a functional relationship between the concentration and the refractive index.
(5) And (4) taking the vitronectin solution, determining the refractive index of the vitronectin solution when the content of the vitronectin is unknown, and calculating the content of the vitronectin according to the functional relation obtained in the step (4).
3. The method of claim 2, wherein the concentration of the boscalid is determined by a refractive index test, and the concentration of the boscalid solution is any reasonable concentration.
4. The method of claim 2, wherein the concentration of the bose in the solution is 10%, 20%, 30%, 40%, 50%, or the like, in a series of bose standard solutions with different concentrations.
5. The method for determining the concentration of the vitronectin according to claim 2, wherein the refractive index is measured at a temperature of 20 ℃ but any temperature which is operable and reasonable can be used as long as all samples (including standard samples and unknown samples) are measured at the same temperature.
6. The method for determining concentration of vitronectin using refractive index test as claimed in claim 2, wherein: the steps (1) and (2) may be exchanged or performed simultaneously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911396367.9A CN111189800A (en) | 2019-12-30 | 2019-12-30 | Method for determining content and concentration of vitronectin by using refractive index test |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911396367.9A CN111189800A (en) | 2019-12-30 | 2019-12-30 | Method for determining content and concentration of vitronectin by using refractive index test |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112255198A (en) * | 2020-10-19 | 2021-01-22 | 西安工程大学 | Method for detecting photosensitivity of substance |
| CN112697933A (en) * | 2020-12-04 | 2021-04-23 | 珀莱雅化妆品股份有限公司 | Method for measuring content of hydroxypropyl tetrahydropyrane triol and diastereoisomer ratio thereof |
| CN112730720A (en) * | 2020-12-28 | 2021-04-30 | 湖州中科院应用技术研究与产业化中心 | Liquid phase detection method for vitronectin in cosmetics |
| CN114397392A (en) * | 2022-01-18 | 2022-04-26 | 宁波中盛产品检测有限公司 | Ion chromatographic analysis method for vitronectin effective substances in cosmetics |
-
2019
- 2019-12-30 CN CN201911396367.9A patent/CN111189800A/en not_active Withdrawn
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112255198A (en) * | 2020-10-19 | 2021-01-22 | 西安工程大学 | Method for detecting photosensitivity of substance |
| CN112255198B (en) * | 2020-10-19 | 2023-07-28 | 西安工程大学 | Method for detecting photosensitivity of substance |
| CN112697933A (en) * | 2020-12-04 | 2021-04-23 | 珀莱雅化妆品股份有限公司 | Method for measuring content of hydroxypropyl tetrahydropyrane triol and diastereoisomer ratio thereof |
| CN112730720A (en) * | 2020-12-28 | 2021-04-30 | 湖州中科院应用技术研究与产业化中心 | Liquid phase detection method for vitronectin in cosmetics |
| CN112730720B (en) * | 2020-12-28 | 2022-05-24 | 湖州中科院应用技术研究与产业化中心 | Liquid phase detection method for vitronectin in cosmetics |
| CN114397392A (en) * | 2022-01-18 | 2022-04-26 | 宁波中盛产品检测有限公司 | Ion chromatographic analysis method for vitronectin effective substances in cosmetics |
| CN114397392B (en) * | 2022-01-18 | 2023-08-11 | 宁波中盛产品检测有限公司 | Ion chromatography analysis method for vitronectin effective matters in cosmetics |
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Application publication date: 20200522 |
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