CN102504037B - Polysaccharide of marine algae and preparation method and application in cigarette thereof - Google Patents
Polysaccharide of marine algae and preparation method and application in cigarette thereof Download PDFInfo
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- CN102504037B CN102504037B CN 201110332968 CN201110332968A CN102504037B CN 102504037 B CN102504037 B CN 102504037B CN 201110332968 CN201110332968 CN 201110332968 CN 201110332968 A CN201110332968 A CN 201110332968A CN 102504037 B CN102504037 B CN 102504037B
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Abstract
The invention discloses polysaccharide of marine algae and a preparation method and application in cigarette thereof. The preparation method includes washing the marine algae clean, drying the marine algae, breaking the marine algae, conducting extraction in water to obtain extracting solution with the weight ratio of the marine algae and water as 1: 50-60, cooling the extracting solution, conducting centrifugal and vacuum filtration on the extracting solution to obtain filtrate, conducting ethanol precipitation on the filtrate, collecting precipitate and freezing and drying the precipitate to obtain the polysaccharide of the marine algae; or conducting separation and purification on the filtrate through a radial flow chromatography and freezing and drying the filtrate to obtain the polysaccharide of the marine algae. The preparation method is simple to operate, easy to control, low in cost and suitable for industrial production. The prepared polysaccharide of the marine algae is applied to the cigarette, enables the smoke and aroma quantity of the cigarette to be increased, shape to the smoke to be good, drying sensation of the smoke to be reduced and mellow full performance, fine smooth flexible degree and body fluid promotion to be improved, and smoke aroma has characteristics.
Description
Technical field
The present invention relates to preparation and the Application Areas of polysaccharide, be specifically related to a kind of marine algae vegetable polysaccharides and preparation method thereof and the application in cigarette.
Background technology
In the production of cigarettes process, in order to improve the physicals of tobacco, strengthen the anti-processibility of tobacco, it is broken that minimizing adds making of man-hour, needs to add the humectant with humectation effect usually.In tobacco leaf (blade) and pipe tobacco, add humectant, can improve the physicals of pipe tobacco, increase toughness, keep moisture, increase the soft degree of tobacco leaf (blade), pipe tobacco, minimizing adds broken, the loss of making of man-hour, and be conducive to product fragrance and send out thoroughly, cigarette quality is had a very important role.
Traditional humectant such as propylene glycol, glycerine and Xylitol etc. have weak point separately: the propenal that the glycerine combustion decomposition produces can make that flue gas is jealous to degenerate, and seldom uses in tobacco.And the very most of dependence import of propylene glycol, price is also high, is unfavorable for reducing the cigarette cost.Though the price of Xylitol, sorbyl alcohol is low, its humectation performance is good not as glycerine and propylene glycol, and is difficult for long storage time.Therefore if can seek that to draw a kind of humectation good but the cheap cigarette with higher use value and application value of price and production extraction cost is a good approach with the humectation that the marine organism material extract is applied to tobacco.
Delivering vegetables and claim Thallus Gracilariae in the sea, is rich in necessary normal, the trace element of multiple human body such as Sargassum polysaccharides, iodine, calcium, iron and Vitamin A, B1, C etc.Sea clearing lung-heat defaecation, the beauty treatment weight reducing of having delivered vegetables, effects such as physical function made in hypotensive blood fat and accent.
Sliver sea lettuce frond sap green, general high 10-12cm also can reach 60cm, irregular y-bend division.Sliver is like ligule or wire, the irregular wavy or cognate shape in edge.Membranous, thick 70-80 μ m.Distribution: near the rock that tide is with and the low tide band is with working energetically damp line in being grown in.Distribution Liaoning, Hebei, Shandong, Zhejiang to Guangdong Hainan Island coast, ground such as Jiangsu are coastal, and the East Sea on the south the Changjiang river and South Sea bank also have growth, but rare gradually by north orientation south.
Sharpening dish (Ulva conglobata) is the large-scale economical alga of South China Sea, is common kind in green laver (Ulva) algae.
Thick net algae (Pachydictyou coriaceum (Holm.) Okam.) frond is green and brown to brown, lobate, membranous, high 10～20 centimetres, wide 0.7～1.5 centimetre, multiple dichotomy, an end ligule or forked, 2 layers of tegumental cells, 1 layer in marrow cell.On the rock below the growth low tide band, 5～July of peak of growing season, the coastal sociales in Zhejiang; Originate in Sheng mountain, Middle St mountain, Yu Shan.China's southeastern coast all has distribution.
Polysaccharide is the high molecular polymer that is formed by connecting by glycosidic link by the monose more than 10, contains a large amount of hydroxyl isopolarity groups in its molecule, can slow down the lost speed of moisture by forming hydrogen bond with water molecules, thereby show certain moisture retention.Some polysaccharide has been applied to tobacco industry at present, played good humectation effect, as disclosed a kind of cactus polyoses among the Chinese patent ZL200910172520.X etc., and the sea deliver vegetables polysaccharide, sliver sea lettuce polysaccharide, sharpen the dish polysaccharide, the application of thick net polysaccharides in cigarette yet there are no report.
Summary of the invention
The invention provides a kind of polysaccharides of marine algae that can be applicable in the cigarette.
The present invention also provides a kind of preparation method of polysaccharides of marine algae, and this method is simple to operate, be easy to control.
The present invention also provides the application of a kind of polysaccharides of marine algae in cigarette, and polysaccharides of marine algae is applied in the cigarette, can significantly improve exquisiteness sense and the lightness of cigarette.
A kind of preparation method of polysaccharides of marine algae may further comprise the steps:
A. it is dry marine algae to be cleaned the back, obtains the marine algae powder through pulverizing;
B. the marine algae powder is extracted in water, obtain extracting solution, wherein, the weight ratio of marine algae powder and water is 1: 50-60;
C. the extracting solution cooling obtains filtrate by centrifugal and suction filtration;
D. adding volume fraction in the filtrate is the ethanol of 95%-100%, makes that the ethanol volume fraction reaches 75%-90% in the system, stirs, and leaves standstill 8h-12h, and centrifugal, collecting precipitation namely gets polysaccharides of marine algae after the lyophilize; Perhaps, filtrate is separated the purification postlyophilization with the radial flow chromatogram method and obtain polysaccharides of marine algae;
Described marine algae is to deliver vegetables in the sea, the sliver sea lettuce, sharpen dish or thick net algae.
Described sea deliver vegetables complete stool, sliver sea lettuce complete stool, sharpen dish complete stool or thick net algae complete stool and all be applicable to the present invention and pluck conveniently that therefore to deliver vegetables be the sea complete stool of delivering vegetables in described sea; Perhaps, described sliver sea lettuce is sliver sea lettuce complete stool; Perhaps, described whetstone dish is to sharpen the dish complete stool; Perhaps, described thick net algae is thick net algae complete stool.The marine algae that all can select for use Zhejiang to produce.
Among the step a, dry mode is varied, can dry naturally, dry in the shade and also can pass through oven for drying, and from saving time and avoiding destroying activeconstituents two aspects and take all factors into consideration, dry temperature is preferably 55 ℃-65 ℃.
Be the effect that further guarantees to extract, preferred:
The order number of described marine algae powder is 20 orders-100 orders.
Among the step b, the condition of described extraction is: 80 ℃ of-100 ℃ of refluxing extraction 2h-4h;
Perhaps, the condition of described extraction is: adopt ultrasonic-assisted extraction, ultrasonic power is 400w-500w, time 20min-30min.
In the steps d, the condition that filtrate is separated purification with the radial flow chromatogram method is: filtrate is mixed with the aqueous solution that filter liquor concentration is 10mg/ml, and last sample flow velocity is 2ml/min, uses the distilled water wash-out, and elution flow rate is 50ml/min.
Described volume fraction is that the ethanol of 95%-100% can adopt the commercially available prod.
Described polysaccharides of marine algae can be applicable in the cigarette, does not need to change the original complete processing of cigarette during application, but can strengthen sophistication and the saturation ratio of cigarette, reduces the dry sensation when sucking.
The consumption of described polysaccharides of marine algae is preferably 0.02 ‰-0.2 ‰ of cigarette shreds quality.
The application method of described polysaccharides of marine algae in cigarette comprises: through water dissolution, the solution spray that is mixed with 0.1mg/ml-1mg/ml is added on the cigarette shreds, obtains containing the pipe tobacco of polysaccharides of marine algae with described polysaccharides of marine algae.Adopt existing cigarette preparation method as after mixing operations such as silk, balance, cigarette, the pipe tobacco that will contain polysaccharides of marine algae is made cigarette.
Compared with prior art, the present invention has following advantage:
The preparation method of polysaccharides of marine algae of the present invention, adopt drying, pulverizing → water extraction → centrifugal, filtration → alcohol precipitation or radial flow chromatogram method to separate purification → freeze-drying → Crude polysaccharides, simple to operate, be easy to control and cost low, pollution-free, need not to change the complete processing of existing cigarette, be suitable for suitability for industrialized production.
Polysaccharides of marine algae of the present invention may be used in the cigarette, add the cigarette of polysaccharides of marine algae of the present invention, the flue gas perfume quantity increases when burning and sucking, form is good, dry sensation reduces, mellow and full sense, fine and smooth soften and the sense of promoting the production of body fluid strengthen, and have improved the shortcoming of China's cigarette product flue gas drying to a certain extent, have actual production meaning and value.
Embodiment
The present invention is described further below in conjunction with embodiment.
Embodiment 1
A, Jiang Hai deliver vegetables dry in the shade after complete stool is cleaned put into 60 ℃ in baking oven down oven dry be crushed to 60 orders, obtain the sea powder of delivering vegetables;
B, the Jiang Hai powder of delivering vegetables adds as the deliver vegetables water of 50 times of amounts of grain weight amount of sea and extracts down 4h in 90 ℃, obtains extracting solution;
C, extracting solution are cooled to room temperature by 10000r/min high speed centrifugation 10min, and the gained supernatant liquor obtains filtrate again behind vacuum filtration;
D, filtrate is mixed with behind the aqueous solution that filter liquor concentration is 10mg/ml to separate with the radial flow chromatogram method purifies: applied sample amount is 100ml, last sample flow velocity is 2ml/min, uses the distilled water wash-out, and elution flow rate is 50ml/min, concentrate after collecting elutriant, lyophilize namely gets the extra large polysaccharide of delivering vegetables again.
Take by weighing the sea polysaccharide of delivering vegetables according to 0.1 ‰ of tobacco quality, the spray solution that is made into 0.1mg/ml with pure water rolls into Cigarette (note props up 1 as cigarette) to pipe tobacco, carry out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h; Sensory evaluating smoking result is: with do not add the sea and deliver vegetables that (note is propped up A as cigarette for the common commercially available Cigarette of polysaccharide, it is 1 identical that pipe tobacco and cigarette prop up) relatively, flue gas fragrance matter promotes and perfume quantity increases, the form of flue gas is good, the dry sensation of flue gas is low, mellow and full sense, fine and smooth soften and the sense of promoting the production of body fluid strengthen, and cigarette perfume (or spice) is characteristic.
Embodiment 2
A, Jiang Hai deliver vegetables dry in the shade after complete stool is cleaned put into 55 ℃ in baking oven down oven dry be crushed to 20 orders, obtain the sea powder of delivering vegetables;
B, the Jiang Hai powder of delivering vegetables adds as the deliver vegetables water of 60 times of amounts of grain weight amount of sea and extracts down 3h in 80 ℃, obtains extracting solution;
C, extracting solution are cooled to room temperature by 10000r/min high speed centrifugation 10min, and the gained supernatant liquor obtains filtrate again behind vacuum filtration;
D, filtrate is mixed with behind the aqueous solution that filter liquor concentration is 10mg/ml to separate with the radial flow chromatogram method purifies: applied sample amount is 100ml, last sample flow velocity is 2ml/min, uses the distilled water wash-out, and elution flow rate is 50ml/min, concentrate after collecting elutriant, lyophilize namely gets the extra large polysaccharide of delivering vegetables again.
Take by weighing the sea polysaccharide of delivering vegetables according to 0.02 ‰ of tobacco quality, the spray solution that is made into 1mg/ml with pure water rolls into Cigarette (note props up 2 as cigarette) to pipe tobacco, carry out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h; Sensory evaluating smoking result is: with do not add the sea deliver vegetables polysaccharide common commercially available Cigarette (note is propped up A as cigarette) relatively, flue gas fragrance matter promotes and perfume quantity increases, and the form of flue gas is good, and the dry sensation of flue gas is low, mellow and full sense, fine and smooth soften and the sense of promoting the production of body fluid strengthen, and cigarette perfume (or spice) is characteristic.
Embodiment 3
A, Jiang Hai deliver vegetables dry in the shade after complete stool is cleaned put into 65 ℃ in baking oven down oven dry be crushed to 100 orders, obtain the sea powder of delivering vegetables;
B, the Jiang Hai powder of delivering vegetables adds as the sea and delivers vegetables the water of 55 times of amounts of grain weight amount in 100 ℃ of following refluxing extraction 2h, obtains extracting solution;
C, extracting solution are cooled to room temperature by 10000r/min high speed centrifugation 10min, and the gained supernatant liquor obtains filtrate again behind vacuum filtration;
D, filtrate is mixed with behind the aqueous solution that filter liquor concentration is 10mg/ml to separate with the radial flow chromatogram method purifies: applied sample amount is 100ml, last sample flow velocity is 2ml/min, uses the distilled water wash-out, and elution flow rate is 50ml/min, concentrate after collecting elutriant, lyophilize namely gets the extra large polysaccharide of delivering vegetables again.
Take by weighing the sea polysaccharide of delivering vegetables according to 0.2 ‰ of tobacco quality, the spray solution that is made into 0.5mg/ml with pure water rolls into Cigarette (note props up 3 as cigarette) to pipe tobacco, carry out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h; Sensory evaluating smoking result is: with do not add the sea deliver vegetables polysaccharide common commercially available Cigarette (note is propped up A as cigarette) relatively, flue gas fragrance matter promotes and perfume quantity increases, and the form of flue gas is good, and the dry sensation of flue gas is low, mellow and full sense, fine and smooth soften and the sense of promoting the production of body fluid strengthen, and cigarette perfume (or spice) is characteristic.
Embodiment 4
A, Jiang Hai deliver vegetables dry in the shade after complete stool is cleaned put into 60 ℃ in baking oven down oven dry be crushed to 40 orders, obtain the sea powder of delivering vegetables;
B, the Jiang Hai powder of delivering vegetables adds as the deliver vegetables water of 55 times of amounts of grain weight amount of sea, puts into the ultrasonic extraction device, ultrasonic power 480w, and supersound extraction 25min obtains extracting solution;
C, with extracting solution cooling by 10000r/min high speed centrifugation 10min, supernatant liquor obtains filtrate behind vacuum filtration;
D, to add volume fraction in the filtrate be 95% ethanol, constantly stirs, and makes that the ethanol volume fraction reaches 85% in the system, leaves standstill 10h, the centrifugal 15min of 4000r/min, and collecting precipitation namely gets the extra large polysaccharide of delivering vegetables after the lyophilize.
The sea that takes by weighing gained by 0.1 ‰ of the tobacco quality polysaccharide of delivering vegetables, spray solution through being mixed with 1mg/ml after the water dissolution rolls into Cigarette (note props up 4 as cigarette) and afterwards carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h to pipe tobacco; Sensory evaluating smoking result is: with do not add the sea deliver vegetables polysaccharide common commercially available Cigarette (note is propped up A as cigarette) relatively, flue gas fragrance matter promotes and perfume quantity increases, and the form of flue gas is good, and the dry sensation of flue gas is low, mellow and full sense, fine and smooth soften and the sense of promoting the production of body fluid strengthen, and cigarette perfume (or spice) is characteristic.
Embodiment 5
A, Jiang Hai deliver vegetables dry in the shade after complete stool is cleaned put into 55 ℃ in baking oven down oven dry be crushed to 100 orders, obtain the sea powder of delivering vegetables;
B, the Jiang Hai powder of delivering vegetables adds as the deliver vegetables water of 50 times of amounts of grain weight amount of sea, puts into the ultrasonic extraction device, ultrasonic power 400w, and supersound extraction 30min obtains extracting solution;
C, with extracting solution cooling by 10000r/min high speed centrifugation 10min, supernatant liquor obtains filtrate behind vacuum filtration;
D, to add volume fraction in the filtrate be 100% ethanol, constantly stirs, and makes that the ethanol volume fraction reaches 75% in the system, leaves standstill 12h, the centrifugal 15min of 4000r/min, and collecting precipitation namely gets the extra large polysaccharide of delivering vegetables after the lyophilize.
By 0.02 ‰ sea that takes by weighing gained of the tobacco quality polysaccharide of delivering vegetables, spray solution through being mixed with 0.1mg/ml after the water dissolution rolls into Cigarette (note props up 5 as cigarette) and afterwards carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h to pipe tobacco; Sensory evaluating smoking result is: with do not add the sea deliver vegetables polysaccharide common commercially available Cigarette (note is propped up A as cigarette) relatively, flue gas fragrance matter promotes and perfume quantity increases, and the form of flue gas is good, and the dry sensation of flue gas is low, mellow and full sense, fine and smooth soften and the sense of promoting the production of body fluid strengthen, and cigarette perfume (or spice) is characteristic.
Embodiment 6
A, Jiang Hai deliver vegetables dry in the shade after complete stool is cleaned put into 65 ℃ in baking oven down oven dry be crushed to 20 orders, obtain the sea powder of delivering vegetables;
B, the Jiang Hai powder of delivering vegetables adds as the deliver vegetables water of 60 times of amounts of grain weight amount of sea, puts into the ultrasonic extraction device, ultrasonic power 500w, and supersound extraction 20min obtains extracting solution;
C, with extracting solution cooling by 10000r/min high speed centrifugation 10min, supernatant liquor obtains filtrate behind vacuum filtration;
D, to add volume fraction in the filtrate be 98% ethanol, constantly stirs, and makes that the ethanol volume fraction reaches 90% in the system, leaves standstill 8h, the centrifugal 15min of 4000r/min, and collecting precipitation namely gets the extra large polysaccharide of delivering vegetables after the lyophilize.
The sea that takes by weighing gained by 0.2 ‰ of the tobacco quality polysaccharide of delivering vegetables, spray solution through being mixed with 0.5mg/ml after the water dissolution rolls into Cigarette (note props up 6 as cigarette) and afterwards carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h to pipe tobacco; Sensory evaluating smoking result is: with do not add the sea deliver vegetables polysaccharide common commercially available Cigarette (note is propped up A as cigarette) relatively, flue gas fragrance matter promotes and perfume quantity increases, and the form of flue gas is good, and the dry sensation of flue gas is low, mellow and full sense, fine and smooth soften and the sense of promoting the production of body fluid strengthen, and cigarette perfume (or spice) is characteristic.
Embodiment 7
A, dry in the shade after sliver sea lettuce complete stool cleaned put into 60 ℃ in baking oven down oven dry be crushed to 60 orders, obtain the full powder of sliver sea lettuce;
B, the water that the full powder of sliver sea lettuce is added as 50 times of amounts of the full grain weight amount of sliver sea lettuce extract 4h down in 90 ℃, obtain extracting solution;
C, extracting solution are cooled to room temperature by 10000r/min high speed centrifugation 10min, and the gained supernatant liquor obtains filtrate again behind vacuum filtration;
D, filtrate is mixed with behind the aqueous solution that filter liquor concentration is 10mg/ml to separate with the radial flow chromatogram method purifies: applied sample amount is 100ml, last sample flow velocity is 2ml/min, uses the distilled water wash-out, and elution flow rate is 50ml/min, concentrate after collecting elutriant, lyophilize namely gets sliver sea lettuce polysaccharide again.
Take by weighing sliver sea lettuce polysaccharide according to 0.1 ‰ of tobacco quality, the spray solution that is made into 0.1mg/ml with pure water rolls into Cigarette (note props up 7 as cigarette) to pipe tobacco, carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add sliver sea lettuce polysaccharide, flue gas fragrance matter promotes and perfume quantity increases, the form of flue gas is good, the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, comfort level, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
Embodiment 8
A, dry in the shade after sliver sea lettuce complete stool cleaned put into 55 ℃ in baking oven down oven dry be crushed to 20 orders, obtain the sliver ulva lactuca powder;
B, the water that the sliver ulva lactuca powder is added as 60 times of amounts of sliver ulva lactuca powder weight extract 3h down in 80 ℃, obtain extracting solution;
C, extracting solution are cooled to room temperature by 10000r/min high speed centrifugation 10min, and the gained supernatant liquor obtains filtrate again behind vacuum filtration;
D, filtrate is mixed with behind the aqueous solution that filter liquor concentration is 10mg/ml to separate with the radial flow chromatogram method purifies: applied sample amount is 100ml, last sample flow velocity is 2ml/min, uses the distilled water wash-out, and elution flow rate is 50ml/min, concentrate after collecting elutriant, lyophilize namely gets sliver sea lettuce polysaccharide again.
Take by weighing sliver sea lettuce polysaccharide according to 0.02 ‰ of tobacco quality, the spray solution that is made into 1mg/ml with pure water rolls into Cigarette (note props up 8 as cigarette) to pipe tobacco, carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add sliver sea lettuce polysaccharide, flue gas fragrance matter promotes and perfume quantity increases, the form of flue gas is good, the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, comfort level, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
Embodiment 9
A, dry in the shade after sliver sea lettuce complete stool cleaned put into 65 ℃ in baking oven down oven dry be crushed to 100 orders, obtain the sliver ulva lactuca powder;
B, the sliver ulva lactuca powder is added as 55 times of amounts of sliver ulva lactuca powder weight water in 100 ℃ of following refluxing extraction 2h, obtain extracting solution;
C, extracting solution are cooled to room temperature by 10000r/min high speed centrifugation 10min, and the gained supernatant liquor obtains filtrate again behind vacuum filtration;
D, filtrate is mixed with behind the aqueous solution that filter liquor concentration is 10mg/ml to separate with the radial flow chromatogram method purifies: applied sample amount is 100ml, last sample flow velocity is 2ml/min, uses the distilled water wash-out, and elution flow rate is 50ml/min, concentrate after collecting elutriant, lyophilize namely gets sliver sea lettuce polysaccharide again.
Take by weighing sliver sea lettuce polysaccharide according to 0.2 ‰ of tobacco quality, the spray solution that is made into 0.5mg/ml with pure water rolls into Cigarette (note props up 9 as cigarette) to pipe tobacco, carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add sliver sea lettuce polysaccharide, flue gas fragrance matter promotes and perfume quantity increases, the form of flue gas is good, the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, comfort level, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
Embodiment 10
A, dry in the shade after sliver sea lettuce complete stool cleaned put into 60 ℃ in baking oven down oven dry be crushed to 40 orders, obtain the sliver ulva lactuca powder;
B, the sliver ulva lactuca powder is added as the water of 55 times of amounts of sliver ulva lactuca powder weight, put into the ultrasonic extraction device, ultrasonic power 480w, supersound extraction 25min obtains extracting solution;
C, with extracting solution cooling by 10000r/min high speed centrifugation 10min, supernatant liquor obtains filtrate behind vacuum filtration;
D, to add volume fraction in the filtrate be 95% ethanol, constantly stirs, and makes that the ethanol volume fraction reaches 85% in the system, leaves standstill 10h, the centrifugal 15min of 4000r/min, and collecting precipitation namely gets sliver sea lettuce polysaccharide after the lyophilize.
Take by weighing the sliver sea lettuce polysaccharide of gained by 0.1 ‰ of tobacco quality, spray solution through being mixed with 1mg/ml after the water dissolution rolls into Cigarette (note props up 10 as cigarette) and afterwards carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h to pipe tobacco; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add sliver sea lettuce polysaccharide, flue gas fragrance matter promotes and perfume quantity increases, the form of flue gas is good, the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, comfort level, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
Embodiment 11
A, dry in the shade after sliver sea lettuce complete stool cleaned put into 55 ℃ in baking oven down oven dry be crushed to 100 orders, obtain the sliver ulva lactuca powder;
B, the sliver ulva lactuca powder is added as the water of 50 times of amounts of sliver ulva lactuca powder weight, put into the ultrasonic extraction device, ultrasonic power 400w, supersound extraction 30min obtains extracting solution;
C, with extracting solution cooling by 10000r/min high speed centrifugation 10min, supernatant liquor obtains filtrate behind vacuum filtration;
D, to add volume fraction in the filtrate be 100% ethanol, constantly stirs, and makes that the ethanol volume fraction reaches 75% in the system, leaves standstill 12h, the centrifugal 15min of 4000r/min, and collecting precipitation namely gets sliver sea lettuce polysaccharide after the lyophilize.
Take by weighing the sliver sea lettuce polysaccharide of gained by 0.02 ‰ of tobacco quality, spray solution through being mixed with 0.1mg/ml after the water dissolution rolls into Cigarette (note props up 11 as cigarette) and afterwards carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h to pipe tobacco; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add sliver sea lettuce polysaccharide, flue gas fragrance matter promotes and perfume quantity increases, the form of flue gas is good, the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, comfort level, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
Embodiment 12
A, dry in the shade after sliver sea lettuce complete stool cleaned put into 65 ℃ in baking oven down oven dry be crushed to 20 orders, obtain the sliver ulva lactuca powder;
B, the sliver ulva lactuca powder is added as the water of 60 times of amounts of sliver ulva lactuca powder weight, put into the ultrasonic extraction device, ultrasonic power 500w, supersound extraction 20min obtains extracting solution;
C, with extracting solution cooling by 10000r/min high speed centrifugation 10min, supernatant liquor obtains filtrate behind vacuum filtration;
D, to add volume fraction in the filtrate be 98% ethanol, constantly stirs, and makes that the ethanol volume fraction reaches 90% in the system, leaves standstill 8h, the centrifugal 15min of 4000r/min, and collecting precipitation namely gets sliver sea lettuce polysaccharide after the lyophilize.
Take by weighing the sliver sea lettuce polysaccharide of gained by 0.2 ‰ of tobacco quality, spray solution through being mixed with 0.5mg/ml after the water dissolution rolls into Cigarette (note props up 12 as cigarette) and afterwards carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h to pipe tobacco; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add sliver sea lettuce polysaccharide, flue gas fragrance matter promotes and perfume quantity increases, the form of flue gas is good, the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, comfort level, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
Embodiment 13
A, will sharpen dry in the shade after the dish complete stool is cleaned put into 60 ℃ in baking oven down oven dry be crushed to 60 orders, obtain sharpening the dish powder;
B, will sharpen the dish powder and add as the water of sharpening 50 times of amounts of dish grain weight amount and extract 4h down in 90 ℃, obtain extracting solution;
C, extracting solution are cooled to room temperature by 10000r/min high speed centrifugation 10min, and the gained supernatant liquor obtains filtrate again behind vacuum filtration;
D, filtrate is mixed with behind the aqueous solution that filter liquor concentration is 10mg/ml to separate with the radial flow chromatogram method purifies: applied sample amount is 100ml, last sample flow velocity is 2ml/min, uses the distilled water wash-out, and elution flow rate is 50ml/min, concentrate after collecting elutriant, lyophilize namely gets and sharpens the dish polysaccharide again.
Take by weighing whetstone dish polysaccharide according to 0.1 ‰ of tobacco quality, the spray solution that is made into 0.1mg/ml with pure water rolls into Cigarette (note props up 13 as cigarette) to pipe tobacco, carry out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add whetstone dish polysaccharide, flue gas fragrance matter promotes and perfume quantity increases, the form of flue gas is good, the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, comfort level, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
Embodiment 14
A, will sharpen dry in the shade after the dish complete stool is cleaned put into 55 ℃ in baking oven down oven dry be crushed to 20 orders, obtain sharpening the dish powder;
B, will sharpen the dish powder and add as the water of sharpening 60 times of amounts of dish grain weight amount and extract 3h down in 80 ℃, obtain extracting solution;
C, extracting solution are cooled to room temperature by 10000r/min high speed centrifugation 10min, and the gained supernatant liquor obtains filtrate again behind vacuum filtration;
D, filtrate is mixed with behind the aqueous solution that filter liquor concentration is 10mg/ml to separate with the radial flow chromatogram method purifies: applied sample amount is 100ml, last sample flow velocity is 2ml/min, uses the distilled water wash-out, and elution flow rate is 50ml/min, concentrate after collecting elutriant, lyophilize namely gets and sharpens the dish polysaccharide again.
Take by weighing whetstone dish polysaccharide according to 0.02 ‰ of tobacco quality, the spray solution that is made into 1mg/ml with pure water rolls into Cigarette (note props up 14 as cigarette) to pipe tobacco, carry out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add whetstone dish polysaccharide, flue gas fragrance matter promotes and perfume quantity increases, the form of flue gas is good, the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, comfort level, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
Embodiment 15
A, will sharpen dry in the shade after the dish complete stool is cleaned put into 65 ℃ in baking oven down oven dry be crushed to 100 orders, obtain sharpening the dish powder;
B, will sharpen the dish powder add as sharpen 55 times of amounts of dish grain weight amount water in 100 ℃ of following refluxing extraction 2h, obtain extracting solution;
C, extracting solution are cooled to room temperature by 10000r/min high speed centrifugation 10min, and the gained supernatant liquor obtains filtrate again behind vacuum filtration;
D, filtrate is mixed with behind the aqueous solution that filter liquor concentration is 10mg/ml to separate with the radial flow chromatogram method purifies: applied sample amount is 100ml, last sample flow velocity is 2ml/min, uses the distilled water wash-out, and elution flow rate is 50ml/min, concentrate after collecting elutriant, lyophilize namely gets and sharpens the dish polysaccharide again.
Take by weighing whetstone dish polysaccharide according to 0.2 ‰ of tobacco quality, the spray solution that is made into 0.5mg/ml with pure water rolls into Cigarette (note props up 15 as cigarette) to pipe tobacco, carry out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add whetstone dish polysaccharide, flue gas fragrance matter promotes and perfume quantity increases, the form of flue gas is good, the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, comfort level, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
Embodiment 16
A, will sharpen dry in the shade after the dish complete stool is cleaned put into 60 ℃ in baking oven down oven dry be crushed to 40 orders, obtain sharpening the dish powder;
B, will sharpen the dish powder and add as the water of sharpening 55 times of amounts of dish grain weight amount, put into the ultrasonic extraction device, ultrasonic power 480w, supersound extraction 25min obtains extracting solution;
C, with extracting solution cooling by 10000r/min high speed centrifugation 10min, supernatant liquor obtains filtrate behind vacuum filtration;
D, to add volume fraction in the filtrate be 95% ethanol, constantly stirs, and makes that the ethanol volume fraction reaches 85% in the system, leaves standstill 10h, the centrifugal 15min of 4000r/min, and collecting precipitation namely gets after the lyophilize and sharpens the dish polysaccharide.
Take by weighing the whetstone dish polysaccharide of gained by 0.1 ‰ of tobacco quality, spray solution through being mixed with 1mg/ml after the water dissolution rolls into Cigarette (note props up 16 as cigarette) and afterwards carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h to pipe tobacco; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add whetstone dish polysaccharide, flue gas fragrance matter promotes and perfume quantity increases, the form of flue gas is good, the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, comfort level, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
Embodiment 17
A, will sharpen dry in the shade after the dish complete stool is cleaned put into 55 ℃ in baking oven down oven dry be crushed to 100 orders, obtain sharpening the dish powder;
B, will sharpen the dish powder and add as the water of sharpening 50 times of amounts of dish grain weight amount, put into the ultrasonic extraction device, ultrasonic power 400w, supersound extraction 30min obtains extracting solution;
C, with extracting solution cooling by 10000r/min high speed centrifugation 10min, supernatant liquor obtains filtrate behind vacuum filtration;
D, to add volume fraction in the filtrate be 100% ethanol, constantly stirs, and makes that the ethanol volume fraction reaches 75% in the system, leaves standstill 12h, the centrifugal 15min of 4000r/min, and collecting precipitation namely gets after the lyophilize and sharpens the dish polysaccharide.
Take by weighing the whetstone dish polysaccharide of gained by 0.02 ‰ of tobacco quality, spray solution through being mixed with 0.1mg/ml after the water dissolution rolls into Cigarette (note props up 17 as cigarette) and afterwards carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h to pipe tobacco; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add whetstone dish polysaccharide, flue gas fragrance matter promotes and perfume quantity increases, the form of flue gas is good, the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, comfort level, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
Embodiment 18
A, will sharpen dry in the shade after the dish complete stool is cleaned put into 65 ℃ in baking oven down oven dry be crushed to 20 orders, obtain sharpening the dish powder;
B, will sharpen the dish powder and add as the water of sharpening 60 times of amounts of dish grain weight amount, put into the ultrasonic extraction device, ultrasonic power 500w, supersound extraction 20min obtains extracting solution;
C, with extracting solution cooling by 10000r/min high speed centrifugation 10min, supernatant liquor obtains filtrate behind vacuum filtration;
D, to add volume fraction in the filtrate be 98% ethanol, constantly stirs, and makes that the ethanol volume fraction reaches 90% in the system, leaves standstill 8h, the centrifugal 15min of 4000r/min, and collecting precipitation namely gets after the lyophilize and sharpens the dish polysaccharide.
Take by weighing the whetstone dish polysaccharide of gained by 0.2 ‰ of tobacco quality, spray solution through being mixed with 0.5mg/ml after the water dissolution rolls into Cigarette (note props up 18 as cigarette) and afterwards carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h to pipe tobacco; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add whetstone dish polysaccharide, flue gas fragrance matter promotes and perfume quantity increases, the form of flue gas is good, the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, comfort level, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
Embodiment 19
A, dry in the shade after thick net algae complete stool cleaned put into 60 ℃ in baking oven down oven dry be crushed to 60 orders, obtain thick net algae powder;
B, the water that thick net algae powder is added as 50 times of amounts of thick net algae grain weight amount extract 4h down in 90 ℃, obtain extracting solution;
C, extracting solution are cooled to room temperature by 10000r/min high speed centrifugation 10min, and the gained supernatant liquor obtains filtrate again behind vacuum filtration;
D, filtrate is mixed with behind the aqueous solution that filter liquor concentration is 10mg/ml to separate with the radial flow chromatogram method purifies: applied sample amount is 100ml, last sample flow velocity is 2ml/min, uses the distilled water wash-out, and elution flow rate is 50ml/min, concentrate after collecting elutriant, lyophilize namely gets thick net polysaccharides again.
Take by weighing thick net polysaccharides according to 0.1 ‰ of tobacco quality, the spray solution that is made into 0.1mg/ml with pure water rolls into Cigarette (note props up 19 as cigarette) to pipe tobacco, carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add thick net polysaccharides, the flue gas perfume quantity increases, and the form of flue gas is good, and the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
Embodiment 20
A, dry in the shade after thick net algae complete stool cleaned put into 55 ℃ in baking oven down oven dry be crushed to 20 orders, obtain thick net algae powder;
B, the water that thick net algae powder is added as 60 times of amounts of thick net algae grain weight amount extract 3h down in 80 ℃, obtain extracting solution;
C, extracting solution are cooled to room temperature by 10000r/min high speed centrifugation 10min, and the gained supernatant liquor obtains filtrate again behind vacuum filtration;
D, filtrate is mixed with behind the aqueous solution that filter liquor concentration is 10mg/ml to separate with the radial flow chromatogram method purifies: applied sample amount is 100ml, last sample flow velocity is 2ml/min, uses the distilled water wash-out, and elution flow rate is 50ml/min, concentrate after collecting elutriant, lyophilize namely gets thick net polysaccharides again.
Take by weighing thick net polysaccharides according to 0.02 ‰ of tobacco quality, the spray solution that is made into 1mg/ml with pure water rolls into Cigarette (note props up 20 as cigarette) to pipe tobacco, carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add thick net polysaccharides, the flue gas perfume quantity increases, and the form of flue gas is good, and the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
Embodiment 21
A, dry in the shade after thick net algae complete stool cleaned put into 65 ℃ in baking oven down oven dry be crushed to 100 orders, obtain thick net algae powder;
B, thick net algae powder is added as 55 times of amounts of thick net algae grain weight amount water in 100 ℃ of following refluxing extraction 2h, obtain extracting solution;
C, extracting solution are cooled to room temperature by 10000r/min high speed centrifugation 10min, and the gained supernatant liquor obtains filtrate again behind vacuum filtration;
D, filtrate is mixed with behind the aqueous solution that filter liquor concentration is 10mg/ml to separate with the radial flow chromatogram method purifies: applied sample amount is 100ml, last sample flow velocity is 2ml/min, uses the distilled water wash-out, and elution flow rate is 50ml/min, concentrate after collecting elutriant, lyophilize namely gets thick net polysaccharides again.
Take by weighing thick net polysaccharides according to 0.2 ‰ of tobacco quality, the spray solution that is made into 0.5mg/ml with pure water rolls into Cigarette (note props up 15 as cigarette) to pipe tobacco, carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add thick net polysaccharides, the flue gas perfume quantity increases, and the form of flue gas is good, and the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
Embodiment 22
A, dry in the shade after thick net algae complete stool cleaned put into 60 ℃ in baking oven down oven dry be crushed to 40 orders, obtain thick net algae powder;
B, thick net algae powder is added as the water of 55 times of amounts of thick net algae grain weight amount, put into the ultrasonic extraction device, ultrasonic power 480w, supersound extraction 25min obtains extracting solution;
C, with extracting solution cooling by 10000r/min high speed centrifugation 10min, supernatant liquor obtains filtrate behind vacuum filtration;
D, to add volume fraction in the filtrate be 95% ethanol, constantly stirs, and makes that the ethanol volume fraction reaches 85% in the system, leaves standstill 10h, the centrifugal 15min of 4000r/min, and collecting precipitation namely gets thick net polysaccharides after the lyophilize.
Take by weighing the thick net polysaccharides of gained by 0.1 ‰ of tobacco quality, spray solution through being mixed with 1mg/ml after the water dissolution rolls into Cigarette (note props up 16 as cigarette) and afterwards carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h to pipe tobacco; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add thick net polysaccharides, the flue gas perfume quantity increases, and the form of flue gas is good, and the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
Embodiment 23
A, dry in the shade after thick net algae complete stool cleaned put into 55 ℃ in baking oven down oven dry be crushed to 100 orders, obtain thick net algae powder;
B, thick net algae powder is added as the water of 50 times of amounts of thick net algae grain weight amount, put into the ultrasonic extraction device, ultrasonic power 400w, supersound extraction 30min obtains extracting solution;
C, with extracting solution cooling by 10000r/min high speed centrifugation 10min, supernatant liquor obtains filtrate behind vacuum filtration;
D, to add volume fraction in the filtrate be 100% ethanol, constantly stirs, and makes that the ethanol volume fraction reaches 75% in the system, leaves standstill 12h, the centrifugal 15min of 4000r/min, and collecting precipitation namely gets thick net polysaccharides after the lyophilize.
Take by weighing the thick net polysaccharides of gained by 0.02 ‰ of tobacco quality, spray solution through being mixed with 0.1mg/ml after the water dissolution rolls into Cigarette (note props up 17 as cigarette) and afterwards carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h to pipe tobacco; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add thick net polysaccharides, the flue gas perfume quantity increases, and the form of flue gas is good, and the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
Embodiment 24
A, dry in the shade after thick net algae complete stool cleaned put into 65 ℃ in baking oven down oven dry be crushed to 20 orders, obtain thick net algae powder;
B, thick net algae powder is added as the water of 60 times of amounts of thick net algae grain weight amount, put into the ultrasonic extraction device, ultrasonic power 500w, supersound extraction 20min obtains extracting solution;
C, with extracting solution cooling by 10000r/min high speed centrifugation 10min, supernatant liquor obtains filtrate behind vacuum filtration;
D, to add volume fraction in the filtrate be 98% ethanol, constantly stirs, and makes that the ethanol volume fraction reaches 90% in the system, leaves standstill 8h, the centrifugal 15min of 4000r/min, and collecting precipitation namely gets thick net polysaccharides after the lyophilize.
Take by weighing the thick net polysaccharides of gained by 0.2 ‰ of tobacco quality, spray solution through being mixed with 0.5mg/ml after the water dissolution rolls into Cigarette (note props up 18 as cigarette) and afterwards carries out the sensory evaluating smoking behind constant temperature (22 ± 1 ℃) constant humidity (relative humidity 60 ± 2%) the balance 48h to pipe tobacco; Sensory evaluating smoking result is: compare with the common commercially available Cigarette (note is propped up A as cigarette) that does not add thick net polysaccharides, the flue gas perfume quantity increases, and the form of flue gas is good, and the dry sensation of flue gas reduces, mellow and full sense, fine and smooth soften, promote the production of body fluid sense and time sweet sense enhancing, cigarette perfume (or spice) is characteristic.
The requirement of smokeing panel test: the smokeing panel test of each sample needs seven and the above persons to participate in two judging panels of cigarette country, five and above provincial judging panel; These qualifications all need to pass qualification examinations.
Smoke panel test with reference to State Standard of the People's Republic of China (GB 5606.4-2005) " cigarette the 4th part: sense organ technical requirements " (Cigarettes-Part 4:Technical requirements for sense-evaluation), 2005-6-17 issue, 2006-1-1 implement, by General Administration of Quality Supervision, Inspection and Quarantine o of the People's Republic of China and Standardization Administration of China's issue.
The contrast cigarette props up sensory evaluating smoking result's (every value is the mean value with 10 personnel's smoking results of smokeing panel test of qualification) that cigarette that (cigarette props up A) and embodiment of the invention 1-24 add polysaccharides of marine algae props up 1-24 and sees the following form 1:
Table 1
Claims (5)
1. the preparation method of a polysaccharides of marine algae may further comprise the steps:
A. it is dry marine algae to be cleaned the back, obtains the marine algae powder through pulverizing, and dry temperature is 55 ℃-65 ℃;
B. the marine algae powder is extracted in water, obtain extracting solution, wherein, the weight ratio of marine algae powder and water is 1:50-60; The condition of described extraction is: 80 ℃ of-100 ℃ of refluxing extraction 2h-4h; Perhaps, the condition of described extraction is: adopt ultrasonic-assisted extraction, ultrasonic power is 400w-500w, time 20min-30min;
C. the extracting solution cooling obtains filtrate by centrifugal and suction filtration;
D. adding volume fraction in the filtrate is the ethanol of 95%-100%, makes that the ethanol volume fraction reaches 75%-90% in the system, stirs, and leaves standstill 8h-12h, and centrifugal, collecting precipitation namely gets polysaccharides of marine algae after the lyophilize; Perhaps, filtrate is separated the purification postlyophilization with the radial flow chromatogram method and obtain polysaccharides of marine algae; Filtrate is separated the condition of purifying with the radial flow chromatogram method is: filtrate is mixed with the aqueous solution that filter liquor concentration is 10mg/ml, and last sample flow velocity is 2ml/min, uses the distilled water wash-out, and elution flow rate is 50ml/min;
Described marine algae is to deliver vegetables in the sea, the sliver sea lettuce, sharpen dish or thick net algae; Delivering vegetables in described sea is the sea complete stool of delivering vegetables; Perhaps, described sliver sea lettuce is sliver sea lettuce complete stool; Perhaps, described whetstone dish is to sharpen the dish complete stool; Perhaps, described thick net algae is thick net algae complete stool;
The order number of described marine algae powder is 20 orders-100 orders.
2. the polysaccharides of marine algae of the preparation method of polysaccharides of marine algae according to claim 1 preparation.
3. the application of polysaccharides of marine algae according to claim 2 in cigarette.
4. the application of polysaccharides of marine algae according to claim 3 in cigarette is characterized in that, the consumption of polysaccharides of marine algae is 0.02 ‰-0.2 ‰ of cigarette shreds quality.
5. according to claim 3 or 4 application of described polysaccharides of marine algae in cigarette, it is characterized in that, described application method comprises: through water dissolution, the solution spray that is mixed with 0.1mg/ml-1mg/ml is added on the cigarette shreds, the preparation cigarette with described polysaccharides of marine algae.
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| CN102940131B (en) * | 2012-12-11 | 2013-09-04 | 江西新世纪民星动物保健品有限公司 | Novel mycotoxin detoxification agent and preparation method thereof |
| CN103082405B (en) * | 2013-01-31 | 2015-03-04 | 中国烟草总公司郑州烟草研究院 | Application of ulva alcohol extract in cigarette |
| CN103054155B (en) * | 2013-01-31 | 2015-02-04 | 中国烟草总公司郑州烟草研究院 | Application of ulva water extract in cigarette |
| CN103070476B (en) * | 2013-01-31 | 2015-03-04 | 中国烟草总公司郑州烟草研究院 | Application of ulva polysaccharide in cigar |
| CN104250309A (en) * | 2014-10-08 | 2014-12-31 | 浙江工业大学 | Ulva fasciata polysaccharide and application thereof |
| CN105167176B (en) * | 2015-08-18 | 2017-05-10 | 浙江中烟工业有限责任公司 | Composite tobacco humectant and composite tobacco humectant preparation method and application of composite tobacco humectant |
| CN105175563A (en) * | 2015-08-18 | 2015-12-23 | 浙江中烟工业有限责任公司 | Extraction method and application of Grateloupia turuturu Yamada polysaccharide |
| CN105254774A (en) * | 2015-11-09 | 2016-01-20 | 湖南炎帝生物工程有限公司 | Nostoc commune polysaccharide extraction method |
| CN105622775A (en) * | 2016-02-29 | 2016-06-01 | 湖南炎帝生物工程有限公司 | Nostoc sphaeroides polysaccharide and extracting method thereof |
| CN113712238B (en) * | 2021-09-03 | 2023-03-10 | 福建中烟工业有限责任公司 | Preparation of coral algae enzymolysis product and application of coral algae enzymolysis product in cigarettes |
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