CN102500129A - Method for high-efficiently realizing separation of soil animals and soil in crude products obtained by Tullgren method - Google Patents
Method for high-efficiently realizing separation of soil animals and soil in crude products obtained by Tullgren method Download PDFInfo
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- CN102500129A CN102500129A CN2011103338482A CN201110333848A CN102500129A CN 102500129 A CN102500129 A CN 102500129A CN 2011103338482 A CN2011103338482 A CN 2011103338482A CN 201110333848 A CN201110333848 A CN 201110333848A CN 102500129 A CN102500129 A CN 102500129A
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- sucrose solution
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Abstract
A method for high-efficiently realizing separation of soil animals and soil in crude products obtained by a Tullgren method relates to a separation method and resolves problems that extraction efficiency of soil animals in crude products obtained by the Tullgren method is low, and the individually small, soil animals with the color similar to that of the soil are not easy to be found. The method includes preparing sucrose solution; adopting the Tullgren method to separate the soil animals, pouring liquid supernatant and collecting the soil animals after a beaker for collecting the soil animals is in standing, adding the sucrose solution into the crude products in the beaker, standing the beaker after being oscillated, pouring liquid into a culture dish and repeatedly using the sucrose solution to realize separation; and taking large-size soil animals, cleaning the large-size soil animals by alcohol, collecting and storing the large-size soil animals, then sucking medium and small-size soil animals, cleaning the medium and small-size animals by alcohol, and collecting and storing the medium and small-size animals after the medium and small-size animals are repeatedly cleaned, so that the steps of the method are finished. The method resolves the problem that the individually small animals with the color similar to that of the soil are not easy to be found, separation efficiency is improved by once to ten times, and statistical accuracy is improved by 5% to 10%.
Description
Technical field
The present invention relates to a kind of method of separation.
Background technology
Soil animal is meant the animal that lives in soil or the face of land The litter layer.Soil animal plays an important role in the residual body of decomposing organism, change soil physico-chemical property, the circulation of promotion soil material and energy conversion process.The research soil animal needs it is separated earlier from field soil.The Tullgren method is the method commonly used of a kind of separated and collected soil animal; It is that the soil that collects is placed on the sieve in 2mm aperture; The sieve top is being covered with the tube that the 25-40W incandescent lamp is housed and is being toasted; A funnel is put in the sieve below, and hopper outlet is transferred a 50mL beaker that 10-20mL alcohol is housed.The interior soil sample temperature of back separator of turning on light raises, and moisture reduces, and soil animal is heated and moves down, and up to leaving soil, falls in the alcohol along funnel, collects soil animal with this.Because diameter is easy to fall in the beaker through sieve less than the soil of 2mm, soil mixes with soil animal, makes that extraction soil animal efficient is low; Individual less and close with native color soil animal is difficult for coming to light; Mask work is heavy, and sample is prone to loss, and statistical error increases.
Summary of the invention
The objective of the invention is to solve that low, individual less and close with the native color soil animal of soil animal extraction efficiency is difficult for found problem in the bullion that the Tullgren method obtains, and the method for soil animal and native high efficiency separation in a kind of bullion that the Tullgren method is obtained is provided.
The method of soil animal and native high efficiency separation in the bullion of Tullgren method acquisition is realized according to the following steps:
One, takes by weighing 500g sucrose and be dissolved in the 500mL distilled water, obtain sucrose solution;
Two, adopt the Tullgren method to separate soil animal; The beaker that to collect soil animal then leaves standstill 1-2h, pours out supernatant then and collects soil animal, adds the sucrose solution of 8～10ml again in the deposition bullion in beaker; There is soil animal to float in the sucrose solution after the vibration; Leave standstill 20～30s, pour out liquid in culture dish, repeat to separate 1～3 time with sucrose solution;
Three, use the large-scale soil animal of tweezers gripping and to use volumetric concentration be that 95% alcohol cleans; Collect and preserve; Use the middle-size and small-size soil animal of suction pipe sucking-off then in another culture dish; Add 20～25mL volumetric concentration and be 95% alcohol and clean, collect after the repeated washing 1～3 time and preserve, promptly accomplish the method for soil animal and native high efficiency separation in the bullion that the Tullgren method is obtained.
Among the present invention the service property (quality) specific concentration be 50% sucrose solution can make in the soil big portion in addition all soil animal separate with soil; Avoided sneaking into soil in the washing sample process; Solved the less and close soil animal of animal individual and be difficult for found problem, improved extraction efficiency and statistical accuracy with native color; Using volumetric concentration is that 95% alcohol cleans to reduce cane sugar content and to improve alcohol concentration repeatedly.The present invention compares with existing separation method, and separative efficiency has improved 1～10 times, and the soil animal loss late is near zero, and statistical accuracy has improved 5%～10%.
The specific embodiment
The specific embodiment one: this embodiment is realized the method for soil animal and native high efficiency separation in the bullion of Tullgren method acquisition according to the following steps:
One, takes by weighing 500g sucrose and be dissolved in the 500mL distilled water, obtain sucrose solution;
Two, adopt the Tullgren method to separate soil animal; The beaker that to collect soil animal then leaves standstill 1-2h, pours out supernatant then and collects soil animal, adds the sucrose solution of 8～10ml again in the deposition bullion in beaker; There is soil animal to float in the sucrose solution after the vibration; Leave standstill 20～30s, pour out liquid in culture dish, repeat to separate 1～3 time with sucrose solution;
Three, use the large-scale soil animal of tweezers gripping and to use volumetric concentration be that 95% alcohol cleans; Collect and preserve; Use the middle-size and small-size soil animal of suction pipe sucking-off then in another culture dish; Add 20～25mL volumetric concentration and be 95% alcohol and clean, collect after the repeated washing 1～3 time and preserve, promptly accomplish the method for soil animal and native high efficiency separation in the bullion that the Tullgren method is obtained.
This embodiment is compared with existing separation method, and separative efficiency has improved 1～10 times, and the soil animal loss late is near zero, and statistical accuracy has improved 5%～10%.
The quality of sucrose solution is 50% than concentration in this embodiment step 1.
Adopting the Tullgren method to separate soil animal in the present embodiment step 2 is routine operation.
This embodiment can also be according to the sucrose concentration that different sample change added; 15% sucrose can make part kind soil animal separate with soil; But, need with more alcohol wash to improve the alcohol concentration of preserving soil animal because water content is 85%.Extract the bigger soil animal of quality and can improve concentration of sucrose to 30% or 70%.
The specific embodiment two: what this embodiment and the specific embodiment one were different is the sucrose solution that adds 9ml in the step 2 in the bullion in beaker.Other step and parameter are identical with the specific embodiment one.
The specific embodiment three: what this embodiment and the specific embodiment one were different is to leave standstill 25s in the step 2, pours out liquid in culture dish, repeats to separate 2 times with sucrose solution.Other step and parameter are identical with the specific embodiment one.
The specific embodiment four: this embodiment and the specific embodiment one are different be in the step 2 in the deposition bullion in the beaker if contain remaining alcohol; The sucrose solution that then adds 8～10ml; The alcohol that vibration back soil animal is suspended in and the joint of sucrose solution; Leave standstill 20～30s, pour out liquid in culture dish, repeat to separate 1～3 time with sucrose solution.Other step and parameter are identical with the specific embodiment one.
The specific embodiment five: this embodiment and the specific embodiment one are different is that to add the 22mL volumetric concentration in the step 3 be that 95% alcohol cleans, and collects after the repeated washing 2 times and preserves.Other step and parameter are identical with the specific embodiment one.
The specific embodiment six: this embodiment and the specific embodiment one are different is that the length of the medium-and-large-sized soil animal of step 3 is 0.01～0.1mm.Other step and parameter are identical with the specific embodiment one.
The specific embodiment seven: this embodiment and the specific embodiment one are different is that the length of middle-size and small-size soil animal in the step 3 is 0.1～1mm.Other step and parameter are identical with the specific embodiment one.
Claims (7)
1. the method for soil animal and native high efficiency separation in the bullion that the Tullgren method is obtained is characterized in that the method for soil animal and native high efficiency separation in the bullion of Tullgren method acquisition is realized according to the following steps:
One, takes by weighing 500g sucrose and be dissolved in the 500mL distilled water, obtain sucrose solution;
Two, adopt the Tullgren method to separate soil animal; The beaker that to collect soil animal then leaves standstill 1-2h, pours out supernatant then and collects soil animal, adds the sucrose solution of 8～10ml again in the deposition bullion in beaker; There is soil animal to float in the sucrose solution after the vibration; Leave standstill 20～30s, pour out liquid in culture dish, repeat to separate 1～3 time with sucrose solution;
Three, use the large-scale soil animal of tweezers gripping and to use volumetric concentration be that 95% alcohol cleans; Collect and preserve; Use the middle-size and small-size soil animal of suction pipe sucking-off then in another culture dish; Add 20～25mL volumetric concentration and be 95% alcohol and clean, collect after the repeated washing 1～3 time and preserve, promptly accomplish the method for soil animal and native high efficiency separation in the bullion that the Tullgren method is obtained.
2. the method for soil animal and native high efficiency separation in a kind of bullion that the Tullgren method is obtained according to claim 1 is characterized in that in the step 2 sucrose solution of adding 9ml in the bullion in beaker.
3. a kind of method with soil animal and native high efficiency separation in the bullion of Tullgren method acquisition according to claim 1 is characterized in that leaving standstill 25s in the step 2, pours out liquid in culture dish, repeats to separate 2 times with sucrose solution.
4. a kind of method according to claim 1 with soil animal and native high efficiency separation in the bullion of Tullgren method acquisition; It is characterized in that in the deposition bullion in the beaker in the step 2 if contain remaining alcohol; The sucrose solution that then adds 8～10ml, 20～30s is left standstill in the alcohol that vibration back soil animal is suspended in and the joint of sucrose solution; Pour out liquid in culture dish, repeat to separate 1～3 time with sucrose solution.
5. the method for soil animal and native high efficiency separation in a kind of bullion that the Tullgren method is obtained according to claim 1 is characterized in that adding in the step 3 22mL volumetric concentration and is 95% alcohol and cleans, and collects preservation after the repeated washing 2 times.
6. a kind of method with soil animal and native high efficiency separation in the bullion of Tullgren method acquisition according to claim 1, the length that it is characterized in that the medium-and-large-sized soil animal of step 3 is 0.01～0.1mm.
7. a kind of method with soil animal and native high efficiency separation in the bullion of Tullgren method acquisition according to claim 1, the length that it is characterized in that middle-size and small-size soil animal in the step 3 is 0.1～1mm.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103338482A CN102500129A (en) | 2011-10-28 | 2011-10-28 | Method for high-efficiently realizing separation of soil animals and soil in crude products obtained by Tullgren method |
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| CN2011103338482A CN102500129A (en) | 2011-10-28 | 2011-10-28 | Method for high-efficiently realizing separation of soil animals and soil in crude products obtained by Tullgren method |
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| CN102500129A true CN102500129A (en) | 2012-06-20 |
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| CN2011103338482A Pending CN102500129A (en) | 2011-10-28 | 2011-10-28 | Method for high-efficiently realizing separation of soil animals and soil in crude products obtained by Tullgren method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103658000A (en) * | 2013-11-28 | 2014-03-26 | 东北师范大学 | SCM-controlled (single-chip microcomputer) soil-animal separator |
| CN104094916A (en) * | 2014-06-17 | 2014-10-15 | 中国科学院动物研究所 | Temperature-adjustable small and medium-sized soil animal separator researched based on landscape architectures and ecological functions |
-
2011
- 2011-10-28 CN CN2011103338482A patent/CN102500129A/en active Pending
Non-Patent Citations (4)
| Title |
|---|
| D.A.CROSSLEY JR.ET AL.: "A high-efficiency,"low-technology"Tullgren-type extractor for soil microarthropods", 《AGRICULTURE,ECOSYSTEMS AND ENVIRONMENT》 * |
| 崔振东: "土壤动物采集方法的介绍", 《动物学杂志》 * |
| 郭建英等: "土壤动物的采集方法", 《生物学通报》 * |
| 陈立杰: "蔗糖离心法分离土壤线虫的最佳条件筛选", 《沈阳农业大学学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103658000A (en) * | 2013-11-28 | 2014-03-26 | 东北师范大学 | SCM-controlled (single-chip microcomputer) soil-animal separator |
| CN104094916A (en) * | 2014-06-17 | 2014-10-15 | 中国科学院动物研究所 | Temperature-adjustable small and medium-sized soil animal separator researched based on landscape architectures and ecological functions |
| CN104094916B (en) * | 2014-06-17 | 2017-02-15 | 中国科学院动物研究所 | Temperature-adjustable small and medium-sized soil animal separator researched based on landscape architectures and ecological functions |
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Application publication date: 20120620 |