CN102499067B - Colored hybrid cotton with aubergine leaves and method for identifying purity and trueness of cotton - Google Patents
Colored hybrid cotton with aubergine leaves and method for identifying purity and trueness of cotton Download PDFInfo
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- CN102499067B CN102499067B CN201110370618.3A CN201110370618A CN102499067B CN 102499067 B CN102499067 B CN 102499067B CN 201110370618 A CN201110370618 A CN 201110370618A CN 102499067 B CN102499067 B CN 102499067B
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Abstract
The invention belongs to the field of agricultural breeding and particularly relates to colored hybrid cotton with aubergine leaves and a method for identifying purity and trueness of the cotton. A male parent adopting anti-insect upland cotton of colored cotton 05-77 with aubergine leaves and a female parent adopting anti-insect low gossypol cotton of male sterile line 10-380A are crossbred for seed production so as to obtain hybrid of F1 generation, namely the colored hybrid cotton with aubergine leaves. The colored hybrid cotton with aubergine leaves has the advantages that the purity of the hybrid can be judged directly according to the aubergine leaf character, and the abnormal plants are removed, so that the hybrid vigor can be exerted completely, the color of produced colored cutton is ensured uniform, and the trueness of the hybrid can be judged according to the character separation proportion. Both parents of the hybrid have mark characters, and abnormal plants can be removed from the parents for production of hybrid seeds according to the marked characters, so that the purpose that the color of the colored hybrid cotton is uniform can be achieved, and the purity of the hybrid can be improved at the same time; besides, the trueness of the hybrid can be judged according to the character separation proportion.
Description
Technical field
The invention belongs to agricultural breeding field, be specifically related to a kind of red autumnal leaves mark color hybrid cotton and purity thereof, true and false and generation other authentication method.
Background technology
The cloth being made into natural color-cotton do not need dyeing, without chemical dye toxin, quality softness and high resilience, the clothes of making are strong to stand up to hard wear, comfortable and easy to wear, favourable health.Because not needing dyeing, so can greatly reduce weaving cost, prevent the pollution of common cotton fabric to environment.Due to these color cotton outstanding advantages, be described as the ecological textile of the tool potentiality in 21 century international market.
China carries out the breeding research of color cotton in early 1990s, start late but develop rapidly, successfully cultivates a collection of by the color cotton new varieties of authorization.Within 1998～2007 years, Chinese color cotton cultivated area expands year by year, and cultivated area is from annual 70hm
2expand 1.3khm to
2above, more than lint yield is increased to 20,000 t from annual 800t, China has become color cotton research scale and has produced the country of utilization maximum.Through tackling key problem in recent years, Breeding of Color Cotton has been obtained breakthrough.Authorized 40 of kinds, wherein 11 of Xinjiang brown cottons, 6 of green cottons by 2009.Brown cotton kind fiber 2.5% span length is 25.3～30.7mm, and specific strength is 20.7～30.7cNtex
-1, micron value is 2.9～4.9; The fiber 2.5% span length 26～31.2mm of green cotton kind, specific strength 12.7～26.3cNtex
-1, micron value 2.5～3.6.The ginning outturn of brown cotton, green cotton kind is respectively 31.3%～38.9% and 23.3%～33.4%, and what have is suitable with white cotton kind.
Although coloured cotton production and breeding have certain progress, still there are some outstanding shortcomings such as ginning outturn is low, bell is little, resistance is poor, fiber is short, brute force is poor, micron value is low.In order to overcome above shortcoming in the short time, the methods of many employing seed selection crossbreed, utilize hybrid vigour to improve color cotton yield and quality, and the kind of authorizing at present, owing to lacking obvious mark property, in seed growing, production of hybrid seeds process, the purity of seed is lacked and effectively identified and control method, on the one hand, hybrid vigour is not in full use and utilizes, on the other hand, the cotton boll blowing such as only have could distinguish to have or not and mix, the cotton color consistency of coloured silk of producing is difficult to guarantee.
Summary of the invention
The object of this invention is to provide a kind of red autumnal leaves mark color hybrid cotton and purity thereof, true and false authentication method, both there is the merit of hybrid cotton, the purity of this hybrid cotton, true and false authentication method can be provided again intuitively.Two parents of this hybrid cotton all have the mark property that can identify in seedling stage, can control from source the purity of hybrid cotton, guarantee that the cotton color and luster of the coloured silk of producing is consistent.
A kind of red autumnal leaves mark color hybrid cotton of the present invention, is obtained by following preparation method:
(1) hybridize with the cotton germplasm line 95-06 of coloured silk of the anti-red autumnal leaves parent of upland cotton and nigger-brown fibre, at F
2in generation, select to have the plant of red autumnal leaves and nigger-brown fibre, in 3～4 generations of selfing continuously, being bred as the color cotton of red autumnal leaves is 99-111 simultaneously;
(2) the color cotton of red autumnal leaves of selecting by step (1) is 99-111 and Insect Resistant Cotton GK-12-5 hybridization, first to F
2carry out resistance to insects evaluation for colony, then in pest-resistant colony, select to have the plant of red autumnal leaves and nigger-brown fibre simultaneously, in continuous 3～4 generations of selfing, are also carried out resistance to insects evaluation, and being bred as the color cotton of pest-resistant upland cotton red autumnal leaves is 05-77;
(3) with pest-resistant caryon sterile line ms-6 and pest-resistant low-phenol cotton strain 7-7 hybridization, at F
2in colony, to low-phenol cotton, sterile strain pollination keeps the low-phenol cotton plant that choosing can be educated, and continuous selfing is also carried out resistance to insects evaluation, to the 4th generation, is bred as pest-resistant low-phenol cotton caryon sterile line 10-380A;
(4) utilizing the color cotton of insect-resistant upland cotton red autumnal leaves that step (2) selects is that 05-77 is the pest-resistant low-phenol cotton caryon sterile line 10-380A that male parent and step (3) select and does the hybridization of female parent production of hybrid seeds, acquisition F
1be red autumnal leaves mark color hybrid cotton for crossbreed.
Wherein, all preferably resist characteristic of disease, yield traits and quality trait and screen in the selfing process in step (1)～(3), wherein, disease resistance is carried out in Infected Field, selects individual plant or plant that anti-blight is good; Yield traits is investigated knot bell, and under per hectare 4.5 ten thousand strain density, 10 of boll per plants are above, Dan Lingchong >=4.5 gram, caryon sterile line ginning outturn more than 38%, color cotton are that ginning outturn more than 32% is standard; Quality trait is according to HVI900 series of tests result, caryon sterile line selection criteria is 2.5% span length >=29.0mm, specific strength >=28.0cN/tex, mic value 3.5～5.0, and color cotton set selection criteria is 2.5% span length >=25.0mm, specific strength >=20.0cN/tex, mic value 3.0～4.0.
Resistance screening methods in step (2) and step (3) is preferably as follows: a leaf period in Cotton Seedling-Growth to 1～3, spread upon in seedling leaves with 4% kanamycin sulfate solution, within seven days, observe and smear blade afterwards, the seedling of blade flavescence is extracted, retained the cotton seedling of normal growth.
Red autumnal leaves mark hybrid cotton Purity method of the present invention, the crossbreed producing in step (4) planted in land for growing field crops or cultivated with incubator, after cotyledon is open and flat, identifying according to Cotyledon color, the leaf look of true crossbreed is red, and the leaf look of false crossbreed is green.
The true and false authentication method of red autumnal leaves mark hybrid cotton of the present invention, the F of the crossbreed producing in results steps (4)
1in generation, carries out selfing and obtains F
2in generation, observe F
2dai Yese, plant have or not phenol and fertility separating ratio, and the ratio of true crossbreed is, redness: green=3: 1, there is phenol strain: without phenol strain=15: and 1, fertile plant: sterile strain=3: 1.
The cotton germplasm line 95-06 of the coloured silk of the anti-red autumnal leaves parent of upland cotton of the present invention, nigger-brown fibre, the color cotton of red autumnal leaves are that 99-111, Insect Resistant Cotton GK-12-5, the pest-resistant color cotton of upland cotton red autumnal leaves are that 05-77, pest-resistant caryon sterile line ms-6, pest-resistant low-phenol cotton strain 7-7 and pest-resistant low-phenol cotton caryon sterile line 10-380A are all purchased from Shandong Nong Xingzhong industry Co., Ltd.
The invention has the advantages that: the inbred line parent who selects has comprehensive good, feature that coordinate force is high, the cotton crossbreed performance of the coloured silk filtering out with it is good.This red autumnal leaves mark hybrid cotton can directly judge according to red autumnal leaves mark property the purity of crossbreed, remove hybrid strain, 100% guarantees heterotic performance, guarantees that the cotton color and luster of the coloured silk of producing is consistent, and can, according to the segregation ratio of proterties, judge the true and false of crossbreed.The parents of this crossbreed all have mark property, can carry out impurity elimination to production of hybrid seeds parent according to mark property, realize color hybrid cotton color and luster consistent, improve the purity of crossbreed simultaneously.
Embodiment
Below in conjunction with embodiment, the present invention will be further described.
Prepare red autumnal leaves mark color hybrid cotton according to following preparation method:
(1) First Year July, with the anti-red autumnal leaves parent of upland cotton and the cotton germplasm line 95-06 hybridization of the coloured silk that is brown fibre, plant F in Hainan winter
1in generation, Second Year, at F
245 of plant selecting simultaneously to have red autumnal leaves and nigger-brown fibre in generation, educate through Nan Fanbei for the 3rd year～the 4th year, and in 4 generations of selfing continuously, the 5th year, being bred as the color cotton of upland cotton red autumnal leaves was 99-111.The color cotton of red autumnal leaves is 14.6 of 99-111 boll per plants, ginning outturn 34.7%, and bell weighs 4.52 grams, 2.5% span length 25.0mm, specific strength 22.50cN/tex, mic value 3.2.
(2) the 5th years is that 99-111 is hybridized with Insect Resistant Cotton GK-12-5 with the color cotton of red autumnal leaves, and plant F in Hainan winter
1generation, the 6th year, first to F
2carry out resistance to insects evaluation for colony, then in pest-resistant colony, select to have 23 of the plant of red autumnal leaves and nigger-brown fibre simultaneously, in the 7th year～the 8th year continuous 4 generations of selfing, were also carried out resistance to insects evaluation, and the 9th year, being bred as pest-resistant upland cotton red autumnal leaves coloured silk cotton was 05-77.The color cotton of pest-resistant upland cotton red autumnal leaves is 05-77 high resistance helicoverpa armigera, 13.8 of boll per plants, and ginning outturn 34.2%, bell weighs 4.61 grams, 2.5% span length 25.6mm, specific strength 23.10cN/tex, mic value 3.1.
(3) the 7th years～the 9th year, with pest-resistant caryon sterile line ms-6 and pest-resistant low-phenol cotton strain 7-7 hybridization, at F
2in colony, the low-phenol cotton plant that choosing can be educated keeps 34 sterile strain pollinations of low-phenol cotton, and continuous selfing is also observed sterile plant rate, to the 4th generation, is bred as pest-resistant low-phenol cotton caryon sterile line 10-380A.Pest-resistant low-phenol cotton caryon sterile line 10-380A high resistance helicoverpa armigera, sterile plant rate 49.8%, plant and seed be without phenol, 14.8 of boll per plants, ginning outturn 43.2%, bell weighs 5.24 grams, 2.5% span length 29.3mm, specific strength 31.50cN/tex, mic value 4.6.
(4) the tenth years, utilizing the color cotton of upland cotton red autumnal leaves is the hybrid combination improved crossing kind that 05-77 is male parent and pest-resistant low-phenol cotton caryon sterile line 10-380A and does hybridization of female parent, at the 11 year variety comparative test, before average yield per mu unginned cotton, frost, before unginned cotton, gined cotton, frost, gined cotton is 228.4 kilograms, 211.8 kilograms, 92.4 kilograms and 86.2 kilograms, grinds No. 28 volume increase 2.9%, 5.0% ,-0.8% and-5.1% respectively than Shandong cotton; Fibre length 30.3mm, specific strength 28.7cN/tex, mic value 4.3.This crossbreed characteristic is to belong to mid-early maturity kind.Emerge, the time of infertility, growing way was sane, medium blade size, redness.125 days breeding times, 108 centimetres of plant heights, plant turriform, the first 7.2 of fruit branch joint positions, 15.1 of fruit branch numbers, 16.2 of boll per plants, bell weighs 5.9 grams, bell oval.Ginning outturn 38.6% before frost, seed refers to 11.0 grams, flower rate 92.7% before frost.Anti-blight, resistance to verticillium wilt, high resistance helicoverpa armigera.
Wherein, in order to improve Breeding Effect, in the selfing process in step (1)～(3), all resist characteristic of disease, yield traits and quality trait and screen, wherein, disease resistance is carried out in Infected Field, selects individual plant or plant that anti-blight is good; Yield traits is investigated knot bell, and under per hectare 4.5 ten thousand strain density, 10 of boll per plants are above, Dan Lingchong >=4.5 gram, caryon sterile line ginning outturn more than 38%, color cotton are that ginning outturn more than 32% is standard; Quality trait is according to HVI900 series of tests result, caryon sterile line selection criteria is 2.5% span length >=29.0mm, specific strength >=28.0cN/tex, mic value 3.5～5.0, and color cotton set selection criteria is 2.5% span length >=25.0mm, specific strength >=20.0cN/tex, mic value 3.0～4.0.
Resistance screening methods in step (2) and step (3) is as follows: a leaf period in Cotton Seedling-Growth to 1～3, spread upon in seedling leaves with 4% kanamycin sulfate solution, within seven days, observe and smear blade afterwards, the seedling of blade flavescence is extracted, retained the cotton seedling of normal growth.
Purity of hybrid, true and false, generation other authentication method: the 11 year, by the crossbreed producing in step (4), within the 12 year, plant F
1in generation, leaf look is all red, and flower portion proterties is identical with normal cotton has no separation.The 13 year, plantation F
2in generation, at land for growing field crops Continuous Observation 560 strain leaf looks and flower portion proterties segregation ratio, result is, redness: green=422: 138, and fertility normal plant: sterile strain=425: 135, segregation ratio meets 3: 1.There is phenol strain: without phenol strain=527: 33, segregation ratio meets 15: 1.
Claims (4)
1. a preparation method for red autumnal leaves mark color hybrid cotton, is characterized in that being obtained by following preparation method:
(1) hybridize with the cotton germplasm line 95-06 of coloured silk of the anti-red autumnal leaves parent of upland cotton and nigger-brown fibre, at F
2in generation, select to have the plant of red autumnal leaves and nigger-brown fibre, in 3～4 generations of selfing continuously, being bred as the color cotton of red autumnal leaves is 99-111 simultaneously;
(2) the color cotton of red autumnal leaves of selecting by step (1) is 99-111 and Insect Resistant Cotton GK-12-5 hybridization, first to F
2carry out resistance to insects evaluation for colony, then in pest-resistant colony, select to have the plant of red autumnal leaves and nigger-brown fibre simultaneously, in continuous 3～4 generations of selfing, are also carried out resistance to insects evaluation, and being bred as the color cotton of pest-resistant upland cotton red autumnal leaves is 05-77;
(3) with pest-resistant caryon sterile line ms-6 and pest-resistant low-phenol cotton strain 7-7 hybridization, at F
2in colony, to low-phenol cotton, sterile strain pollination keeps the low-phenol cotton plant that choosing can be educated, and continuous selfing is also carried out resistance to insects evaluation, to the 4th generation, is bred as pest-resistant low-phenol cotton caryon sterile line 10-380A;
(4) utilizing the color cotton of insect-resistant upland cotton red autumnal leaves that step (2) selects is that 05-77 is the pest-resistant low-phenol cotton caryon sterile line 10-380A that male parent and step (3) select and does the hybridization of female parent production of hybrid seeds, acquisition F
1be red autumnal leaves mark color hybrid cotton for crossbreed;
In selfing process in step (1)～(3), all resist characteristic of disease, yield traits and quality trait and screen, wherein, disease resistance is carried out in Infected Field, selects individual plant or plant that anti-blight is good; Yield traits is investigated knot bell, and under per hectare 4.5 ten thousand strain density, 10 of boll per plants are above, Dan Lingchong >=4.5 gram, caryon sterile line ginning outturn more than 38%, color cotton are that ginning outturn more than 32% is standard; Quality trait is according to HVI900 series of tests result, caryon sterile line selection criteria is 2.5% span length >=29.0mm, specific strength >=28.0cN/tex, mic value 3.5～5.0, and color cotton set selection criteria is 2.5% span length >=25.0mm, specific strength >=20.0cN/tex, mic value 3.0～4.0.
2. the preparation method of red autumnal leaves mark color hybrid cotton according to claim 1, it is characterized in that the resistance screening methods in step (2) and step (3) is: a leaf period in Cotton Seedling-Growth to 1～3, spread upon in seedling leaves with 4% kanamycin sulfate solution, within seven days, observe and smear blade afterwards, the seedling of blade flavescence is extracted, retained the cotton seedling of normal growth.
3. the Purity method of red autumnal leaves mark color hybrid cotton described in a claim 1, it is characterized in that the crossbreed obtaining in claim 1 step (4) to plant in land for growing field crops or cultivate with incubator, after cotyledon is open and flat, identify according to Cotyledon color, the leaf look of true crossbreed is red, and the leaf look of false crossbreed is green.
4. a true and false authentication method for the mark of red autumnal leaves described in claim 1 color hybrid cotton, is characterized in that the F obtaining in claim 1 step (4)
1carry out selfing for crossbreed and obtain F
2in generation, observe F
2dai Yese, plant have or not phenol and fertility separating ratio, and the ratio of true crossbreed is that redness: green=3:1, has phenol strain: without phenol strain=15:1, and fertile plant: sterile strain=3:1.
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| CN102499067B true CN102499067B (en) | 2014-05-28 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103392587A (en) * | 2013-06-26 | 2013-11-20 | 江苏省农业科学院 | Breeding method for improving lint percentage and micronaire value of green fiber cotton |
| CN103493728B (en) * | 2013-08-28 | 2016-04-06 | 山东农兴种业有限责任公司 | The color cotton sterile line breeding of a kind of pest-resistant low phenol and hybrid breeding method |
| CN103493724B (en) * | 2013-08-28 | 2016-04-27 | 山东农兴种业有限责任公司 | The cotton seed selection of a kind of pest-resistant low phenol stigma appearing double-line hybrid and cross-breeding method |
| CN103477972B (en) * | 2013-09-13 | 2016-04-27 | 山东农兴种业有限责任公司 | A kind of red autumnal leaves mark restorer and three line hybrid seed selection thereof |
| CN108935073B (en) * | 2018-07-18 | 2021-08-13 | 邯郸市农业科学院 | Seed production method of purplish red marked three-line hybrid cotton |
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