CN102499067A - Colored hybrid cotton with aubergine leaves and method for identifying purity and trueness of cotton - Google Patents
Colored hybrid cotton with aubergine leaves and method for identifying purity and trueness of cotton Download PDFInfo
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- CN102499067A CN102499067A CN2011103706183A CN201110370618A CN102499067A CN 102499067 A CN102499067 A CN 102499067A CN 2011103706183 A CN2011103706183 A CN 2011103706183A CN 201110370618 A CN201110370618 A CN 201110370618A CN 102499067 A CN102499067 A CN 102499067A
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Abstract
The invention belongs to the field of agricultural breeding and particularly relates to colored hybrid cotton with aubergine leaves and a method for identifying purity and trueness of the cotton. A male parent adopting anti-insect upland cotton of colored cotton 05-77 with aubergine leaves and a female parent adopting anti-insect low gossypol cotton of male sterile line 10-380A are crossbred for seed production so as to obtain hybrid of F1 generation, namely the colored hybrid cotton with aubergine leaves. The colored hybrid cotton with aubergine leaves has the advantages that the purity of the hybrid can be judged directly according to the aubergine leaf character, and the abnormal plants are removed, so that the hybrid vigor can be exerted completely, the color of produced colored cutton is ensured uniform, and the trueness of the hybrid can be judged according to the character separation proportion. Both parents of the hybrid have mark characters, and abnormal plants can be removed from the parents for production of hybrid seeds according to the marked characters, so that the purpose that the color of the colored hybrid cotton is uniform can be achieved, and the purity of the hybrid can be improved at the same time; besides, the trueness of the hybrid can be judged according to the character separation proportion.
Description
Technical field
The invention belongs to the agricultural breeding field, be specifically related to a kind of red autumnal leaves mark color hybrid cotton and purity thereof, true and false and the generation other authentication method.
Background technology
The cloth that is made into natural color-cotton need not dye, not have the chemical dye toxin, quality softness and high resilience, and the clothes of processing are strong to stand up to hard wear, comfortable and easy to wear, favourable health.Because of not needing dyeing,, prevented of the pollution of common cotton fabric to environment so can reduce the weaving cost greatly.Because these color cotton outstanding advantages, be described as the ecological textile of the tool potentiality in 21 century international market.
China carries out colored cotton breeding research in early 1990s, starts late but develop rapidly, successfully cultivates a collection of colored cotton new varieties through authorizing.1998～2007 years Chinese colored cotton seeds are planted area and are enlarged year by year, and cultivated area is from the 70hm in every year
2Expand 1.3khm to
2More than, lint yield is increased to more than 20,000 t from the 800t in every year, and China has become the colored cotton research scale country maximum with producing utilization.Through tackling key problem in recent years, colored cotton breeding has obtained breakthrough.Authorized 40 of kinds by 2009, wherein the Xinjiang brown cotton is 11,6 of green cottons.Brown cotton kind fiber 2.5% span length is 25.3～30.7mm, and specific strength is 20.7～30.7cNtex
-1, micron value is 2.9～4.9; The fiber 2.5% span length 26～31.2mm of green cotton kind, specific strength 12.7～26.3cNtex
-1, micron value 2.5～3.6.The ginning outturn of brown cotton, green cotton kind is respectively 31.3%～38.9% and 23.3%～33.4%, and what have is suitable with the white cotton kind.
Although color cotton production and breeding have certain progress, still there are some outstanding shortcomings such as ginning outturn is low, bell is little, resistance is poor, fiber is short, brute force is poor, micron value is low.In order to overcome above shortcoming in the short time, adopt the method for seed selection crossbreed more, utilize hybrid vigour to improve color cotton yield and quality; And the kind of authorizing at present, because the lack of significant mark property, in seed growing, production of hybrid seeds process; Purity to seed lacks effectively evaluation and control method, and on the one hand, hybrid vigour is not in full use and utilizes; On the other hand, cotton boll blowing such as have only could distinguish to have or not and mix, the color cotton color consistency of producing is difficult to guarantee.
Summary of the invention
The purpose of this invention is to provide a kind of red autumnal leaves mark color hybrid cotton and purity thereof, true and false authentication method, both had the merit of hybrid cotton, again the purity of this hybrid cotton, true and false authentication method can be provided intuitively.Two parents of this hybrid cotton all have the mark property that can discern in seedling stage, can control the purity of hybrid cotton from the source, guarantee that the color cotton color and luster of producing is consistent.
A kind of red autumnal leaves mark color hybrid cotton of the present invention is obtained by following preparation method:
(1) hybridizes with the color cotton germplasm line 95-06 of anti-red autumnal leaves parent of upland cotton and nigger-brown fibre, at F
2Select to have simultaneously the plant of red autumnal leaves and nigger-brown fibre in generation, continuously 3～4 generations of selfing, breeding red autumnal leaves coloured silk cotton is 99-111;
(2) the color cotton of selecting with step (1) of red autumnal leaves is 99-111 and Insect Resistant Cotton GK-12-5 hybridization, at first to F
2Carry out resistance to insects for colony and identify, then in pest-resistant colony, select to have simultaneously the plant of red autumnal leaves and nigger-brown fibre, in continuous 3～4 generations of selfing, also carry out resistance to insects and identify, breeding the color cotton of pest-resistant upland cotton red autumnal leaves is 05-77;
(3) with pest-resistant caryon sterile line ms-6 and pest-resistant low-phenol cotton strain 7-7 hybridization, at F
2In colony, sterile strain pollination keeps the low-phenol cotton plant that choosing can be educated to low-phenol cotton, and continuous selfing is also carried out resistance to insects and identified, to the 4th generation, breeds pest-resistant low-phenol cotton caryon sterile line 10-380A;
(4) utilizing the color cotton of pest-resistant upland cotton red autumnal leaves that step (2) selects is that 05-77 is the pest-resistant low-phenol cotton caryon sterile line 10-380A that male parent and step (3) select and does the hybridization of female parent production of hybrid seeds, acquisition F
1Be red autumnal leaves mark color hybrid cotton for crossbreed.
Wherein, all preferably resist characteristic of disease, yield traits and quality trait in the selfing process in step (1)～(3) and screen, wherein, disease resistance is carried out in sick naturally garden, selects good individual plant of anti-blight or plant; Yield traits is investigated knot bell property, and under per hectare 4.5 ten thousand strain density, boll per plant more than 10, Dan Lingchong >=4.5 grams, caryon sterile line ginning outturn 38% or more, color cotton are that ginning outturn is standard more than 32%; Quality trait is according to HVI900 series of tests result; The caryon sterile line selection criteria is 2.5% span length >=29.0mm, specific strength >=28.0cN/tex, mic value 3.5～5.0, and color cotton set selection criteria is 2.5% span length >=25.0mm, specific strength >=20.0cN/tex, mic value 3.0～4.0.
Resistance to insects authentication method in step (2) and the step (3) is preferably following: grow into 1～3 leaf period at cotton seedling; Kanamycin sulfate solution with 4% spreads upon on the seedling leaves; Observe after seven days and smear blade, the seedling of blade flavescence is extracted, keep the cotton seedling of normal growth.
Red autumnal leaves mark hybrid cotton purity authentication method of the present invention; The crossbreed that produces in the step (4) planted in the land for growing field crops or with incubator cultivate, after cotyledon is open and flat, identify according to the cotyledon color; The leaf look of true crossbreed is red, and the leaf look of false crossbreed is green.
The true and false authentication method of red autumnal leaves mark hybrid cotton of the present invention, the F of the crossbreed that produces in the results steps (4)
1In generation, carries out selfing and obtains F
2In generation, observe F
2Dai Yese, plant have or not phenol and fertility separating ratio, and the ratio of true crossbreed is redness: green=3: 1, the phenol strain is arranged: and no phenol strain=15: 1, can educate strain: sterile strain=3: 1.
The color cotton of the color cotton germplasm line 95-06 of the anti-red autumnal leaves parent of upland cotton of the present invention, nigger-brown fibre, red autumnal leaves is that 99-111, Insect Resistant Cotton GK-12-5, the pest-resistant color cotton of upland cotton red autumnal leaves are that 05-77, pest-resistant caryon sterile line ms-6, pest-resistant low-phenol cotton strain 7-7 and pest-resistant low-phenol cotton caryon sterile line 10-380A all purchase in Shandong emerging kinds of farming Co., Ltd already.
The invention has the advantages that: the inbred line parent who selects has comprehensive good, characteristics that coordinate force is high, and is good with its color cotton crossbreed performance that filters out.This red autumnal leaves mark hybrid cotton can directly be judged the purity of crossbreed according to the red autumnal leaves mark property; Remove assorted strain, 100% guarantees heterotic performance, guarantees that the color cotton color and luster of producing is consistent; And can judge the true and false of crossbreed according to the segregation ratio of proterties.The parents of this crossbreed all have mark property, can carry out impurity elimination to production of hybrid seeds parent according to mark property, realize that the color hybrid cotton color and luster is consistent, improve the purity of crossbreed simultaneously.
Embodiment
Below in conjunction with embodiment the present invention is further specified.
Prepare red autumnal leaves mark color hybrid cotton according to following preparation method:
In July, (1) first, with anti-red autumnal leaves parent of upland cotton and the color cotton germplasm line 95-06 hybridization that is brown fibre, plant F in Hainan winter
1Generation, 1 year, at F
2Select to have simultaneously 45 of the plant of red autumnal leaves and nigger-brown fibre in generation, educated through Nan Fanbei in the 3rd year～the 4th year, continuously 4 generations of selfing, the 5th year, breeding upland cotton red autumnal leaves coloured silk cotton was 99-111.The color cotton of red autumnal leaves is 14.6 of 99-111 boll per plants, ginning outturn 34.7%, and bell weighs 4.52 grams, 2.5% span length 25.0mm, specific strength 22.50cN/tex, mic value 3.2.
(2) the 5th years is 99-111 and Insect Resistant Cotton GK-12-5 hybridization with the color cotton of red autumnal leaves, and plant F in Hainan winter
1Generation, the 6th year, at first to F
2Carry out resistance to insects for colony and identify, then in pest-resistant colony, select to have simultaneously 23 of the plant of red autumnal leaves and nigger-brown fibre, in the 7th year～the 8th year continuous 4 generations of selfing, also carried out the resistance to insects evaluation, and the 9th year, breeding pest-resistant upland cotton red autumnal leaves coloured silk cotton was 05-77.The color cotton of pest-resistant upland cotton red autumnal leaves is the 05-77 high resistance helicoverpa armigera, 13.8 of boll per plants, and ginning outturn 34.2%, bell weighs 4.61 grams, 2.5% span length 25.6mm, specific strength 23.10cN/tex, mic value 3.1.
(3) the 7th years～the 9th year, with pest-resistant caryon sterile line ms-6 and pest-resistant low-phenol cotton strain 7-7 hybridization, at F
2In colony, the low-phenol cotton plant that choosing can be educated keeps 34 sterile strain pollinations of low-phenol cotton, and continuous selfing is also observed sterile plant rate, to the 4th generation, breeds pest-resistant low-phenol cotton caryon sterile line 10-380A.Pest-resistant low-phenol cotton caryon sterile line 10-380A high resistance helicoverpa armigera, sterile plant rate 49.8%, plant and seed do not have phenol, 14.8 of boll per plants, ginning outturn 43.2%, bell weighs 5.24 grams, 2.5% span length 29.3mm, specific strength 31.50cN/tex, mic value 4.6.
(4) the tenth years; Utilizing the color cotton of upland cotton red autumnal leaves is that 05-77 does the hybrid combination improved crossing kind that male parent and pest-resistant low-phenol cotton caryon sterile line 10-380A do hybridization of female parent; At the 11 year variety comparative test; Unginned cotton, gined cotton, the preceding gined cotton of frost are 228.4 kilograms, 211.8 kilograms, 92.4 kilograms and 86.2 kilograms before average yield per mu unginned cotton, the frost, grind No. 28 raising the output 2.9%, 5.0% ,-0.8% and-5.1% than Shandong cotton respectively; Fibre length 30.3mm, specific strength 28.7cN/tex, mic value 4.3.This crossbreed characteristic is to belong to the mid-early maturity kind.Emerge, the time of infertility, growing way was sane, medium blade size, red.125 days breeding times, 108 centimetres of plant heights, the plant turriform, 7.2 of first fruit branch joint positions, several 15.1 of fruit branch, 16.2 of boll per plants, bell weigh 5.9 grams, bell oval.Ginning outturn 38.6% before the frost, seed refer to 11.0 grams, flower rate 92.7% before the frost.Anti-blight, anti-verticillium wilt, high resistance helicoverpa armigera.
Wherein, in order to improve the seed selection effect, all resist characteristic of disease, yield traits and quality trait in the selfing process in step (1)～(3) and screen, wherein, disease resistance is carried out in sick naturally garden, selects good individual plant of anti-blight or plant; Yield traits is investigated knot bell property, and under per hectare 4.5 ten thousand strain density, boll per plant more than 10, Dan Lingchong >=4.5 grams, caryon sterile line ginning outturn 38% or more, color cotton are that ginning outturn is standard more than 32%; Quality trait is according to HVI900 series of tests result; The caryon sterile line selection criteria is 2.5% span length >=29.0mm, specific strength >=28.0cN/tex, mic value 3.5～5.0, and color cotton set selection criteria is 2.5% span length >=25.0mm, specific strength >=20.0cN/tex, mic value 3.0～4.0.
Resistance to insects authentication method in step (2) and the step (3) is following: grow into 1～3 leaf period at cotton seedling; Kanamycin sulfate solution with 4% spreads upon on the seedling leaves; Observe after seven days and smear blade, the seedling of blade flavescence is extracted, keep the cotton seedling of normal growth.
Purity of hybrid, true and false, generation other authentication method: the 11 year, the crossbreed that produces in (4) set by step, the 12 year plantation F
1In generation, the leaf look all is red, and flower portion proterties is identical with normal cotton does not see and separate.The 13 year, plantation F
2Generation, observe 560 strain leaf looks and flower portion proterties segregation ratio continuously in the land for growing field crops, the result is redness: green=422: 138, the fertility normal plant: sterile strain=425: 135, segregation ratio meets 3: 1.The phenol strain is arranged: no phenol strain=527: 33, segregation ratio meets 15: 1.
Claims (5)
1. red autumnal leaves mark color hybrid cotton is characterized in that being obtained by following preparation method:
(1) hybridizes with the color cotton germplasm line 95-06 of anti-red autumnal leaves parent of upland cotton and nigger-brown fibre, at F
2Select to have simultaneously the plant of red autumnal leaves and nigger-brown fibre in generation, continuously 3～4 generations of selfing, breeding red autumnal leaves coloured silk cotton is 99-111;
(2) the color cotton of selecting with step (1) of red autumnal leaves is 99-111 and Insect Resistant Cotton GK-12-5 hybridization, at first to F
2Carry out resistance to insects for colony and identify, then in pest-resistant colony, select to have simultaneously the plant of red autumnal leaves and nigger-brown fibre, in continuous 3～4 generations of selfing, also carry out resistance to insects and identify, breeding the color cotton of pest-resistant upland cotton red autumnal leaves is 05-77;
(3) with pest-resistant caryon sterile line ms-6 and pest-resistant low-phenol cotton strain 7-7 hybridization, at F
2In colony, sterile strain pollination keeps the low-phenol cotton plant that choosing can be educated to low-phenol cotton, and continuous selfing is also carried out resistance to insects and identified, to the 4th generation, breeds pest-resistant low-phenol cotton caryon sterile line 10-380A;
(4) utilizing the color cotton of pest-resistant upland cotton red autumnal leaves that step (2) selects is that 05-77 is the pest-resistant low-phenol cotton caryon sterile line 10-380A that male parent and step (3) select and does the hybridization of female parent production of hybrid seeds, acquisition F
1Be red autumnal leaves mark color hybrid cotton for crossbreed.
2. red autumnal leaves mark hybrid cotton according to claim 1; It is characterized in that all preferably resisting in the selfing process in step (1)～(3) characteristic of disease, yield traits and quality trait screens; Wherein, disease resistance is carried out in sick naturally garden, selects good individual plant of anti-blight or plant; Yield traits is investigated knot bell property, and under per hectare 4.5 ten thousand strain density, boll per plant more than 10, Dan Lingchong >=4.5 grams, caryon sterile line ginning outturn 38% or more, color cotton are that ginning outturn is standard more than 32%; Quality trait is according to HVI900 series of tests result; The caryon sterile line selection criteria is 2.5% span length >=29.0mm, specific strength >=28.0cN/tex, mic value 3.5～5.0, and color cotton set selection criteria is 2.5% span length >=25.0mm, specific strength >=20.0cN/tex, mic value 3.0～4.0.
3. red autumnal leaves mark hybrid cotton according to claim 1; It is characterized in that the resistance to insects authentication method in step (2) and the step (3) is: grow into 1～3 leaf period at cotton seedling; Kanamycin sulfate solution with 4% spreads upon on the seedling leaves; Observe after seven days and smear blade, the seedling of blade flavescence is extracted, keep the cotton seedling of normal growth.
4. the described red autumnal leaves mark of claim 1 hybrid cotton purity authentication method; It is characterized in that the crossbreed that produces in the step (4) planted in the land for growing field crops or with incubator and cultivate; After cotyledon is open and flat; Identify that according to the cotyledon color leaf look of true crossbreed is red, the leaf look of false crossbreed is green.
5. the true and false authentication method of the described red autumnal leaves mark of claim 1 hybrid cotton is characterized in that gathering in the crops the crossbreed F that produces in the step (4)
1In generation, carries out selfing and obtains F
2In generation, observe F
2Dai Yese, plant have or not phenol and fertility separating ratio, and the ratio of true crossbreed is redness: green=3: 1, the phenol strain is arranged: and no phenol strain=15: 1, can educate strain: sterile strain=3: 1.
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Cited By (5)
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| CN103392587A (en) * | 2013-06-26 | 2013-11-20 | 江苏省农业科学院 | Breeding method for improving lint percentage and micronaire value of green fiber cotton |
| CN103477972A (en) * | 2013-09-13 | 2014-01-01 | 山东农兴种业有限责任公司 | Red leaf marking restoring line and three-line crossbreed selection and breeding method thereof |
| CN103493728A (en) * | 2013-08-28 | 2014-01-08 | 山东农兴种业有限责任公司 | Sterile line selective-breeding and hybrid breeding method of pest-resistant and low-gossypol natural colored cotton |
| CN103493724A (en) * | 2013-08-28 | 2014-01-08 | 山东农兴种业有限责任公司 | Seed selection and hybrid seed production method of pest-resistant, low-gossypol and stigma-exposed bilinear hybrid cotton |
| CN108935073A (en) * | 2018-07-18 | 2018-12-07 | 邯郸市农业科学院 | A kind of producing method for seed of the three-line cotton of purplish red color marker |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392587A (en) * | 2013-06-26 | 2013-11-20 | 江苏省农业科学院 | Breeding method for improving lint percentage and micronaire value of green fiber cotton |
| CN103493728A (en) * | 2013-08-28 | 2014-01-08 | 山东农兴种业有限责任公司 | Sterile line selective-breeding and hybrid breeding method of pest-resistant and low-gossypol natural colored cotton |
| CN103493724A (en) * | 2013-08-28 | 2014-01-08 | 山东农兴种业有限责任公司 | Seed selection and hybrid seed production method of pest-resistant, low-gossypol and stigma-exposed bilinear hybrid cotton |
| CN103493724B (en) * | 2013-08-28 | 2016-04-27 | 山东农兴种业有限责任公司 | The cotton seed selection of a kind of pest-resistant low phenol stigma appearing double-line hybrid and cross-breeding method |
| CN103477972A (en) * | 2013-09-13 | 2014-01-01 | 山东农兴种业有限责任公司 | Red leaf marking restoring line and three-line crossbreed selection and breeding method thereof |
| CN103477972B (en) * | 2013-09-13 | 2016-04-27 | 山东农兴种业有限责任公司 | A kind of red autumnal leaves mark restorer and three line hybrid seed selection thereof |
| CN108935073A (en) * | 2018-07-18 | 2018-12-07 | 邯郸市农业科学院 | A kind of producing method for seed of the three-line cotton of purplish red color marker |
| CN108935073B (en) * | 2018-07-18 | 2021-08-13 | 邯郸市农业科学院 | Seed production method of purplish red marked three-line hybrid cotton |
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