CN108901832A - A kind of two-way cross method of quinoa - Google Patents
A kind of two-way cross method of quinoa Download PDFInfo
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- CN108901832A CN108901832A CN201811011523.0A CN201811011523A CN108901832A CN 108901832 A CN108901832 A CN 108901832A CN 201811011523 A CN201811011523 A CN 201811011523A CN 108901832 A CN108901832 A CN 108901832A
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- quinoa
- plant
- way cross
- maternal plant
- gynoecium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of two-way cross methods of quinoa, are related to crop breeding technical field；Solves the problem of quinoa crossing work large labor intensity, emasculation is not thorough and injures gynoecium and lead to hybridization failure；Technical solution is：The paternal plant and maternal plant that selection needs to hybridize, the two-way cross of quinoa is carried out by the method for artificial pollination；The flower for the maternal plant that the two-way cross uses is gynoecium prior to stamen maturation, and flower sufficiently opens when and stamen also prematurity mature in gynoecium, keeps gynoecium all exposed；The present invention can break through many and diverse and later period breeding trouble of quinoa artificial emasculation using specific quinoa maternal plant, only needing to match male parent according to breeding objective can be with hybridization target be realized, this method is the breakthrough of quinoa breeding technique, reaches quick, accurately, efficiently.
Description
Technical field
The present invention relates to crop breeding technical field, the two-way cross method of specially a kind of quinoa.
Background technique
Quinoa（Chenopodium quinoa willd）Belong to Chenopodiaceae, dicotyledonous, androgyny, self-pollination, it was reported that
Outcrossing rate 5-10%.Quinoa, by breeder's concern or nearly 30 years things, just has breeder to open in nearest more than 10 years as cereal crops
Open up quinoa crossbreeding.But the cross breeding method taken at present is still based on artificial emasculation, and then the method for artificial pollination obtains
Filial generation is obtained, by selecting the homozygous inbred lines with merit from offspring.
From the point of view of the Hybrid of incubation, the heterosis, hybrid vigor of quinoa is obvious, therefore the crossbreeding of quinoa can be changed in future
Become quinoa shape, improve yield, improve quality and various resistances and innovation source of variation it is indispensable it is important by way of.But
It is 1, quinoa flower very little that quinoa hybridization, which currently encounters maximum problem, and artificial emasculation difficulty is big, time-consuming；It will receive various aspects
Limitation, such as the qualification of technician, the quality of eyesight, emasculation process, all can be to hybridizing successfully to the damaged condition of floral organ
Rate has an impact；2, for other methods as having full flower using some quinoa kinds, the characteristic of imperfect flower takes part emasculation to carry out
Hybridization, success rate is relatively low, is difficult to meet hybridization demand mostly.
Summary of the invention
The present invention overcomes the deficiencies of the prior art and provide a kind of two-way cross method of convenient and practical quinoa, solves
The problem of quinoa crossing work large labor intensity, emasculation is not thorough and injures gynoecium and lead to hybridization failure.
In order to solve the above technical problems, the technical scheme adopted by the invention is as follows.
A kind of two-way cross method of quinoa, the paternal plant and maternal plant that selection needs to hybridize, passes through artificial pollination
Method carry out quinoa two-way cross；The flower for the maternal plant that the two-way cross uses for gynoecium prior to stamen at
Flower sufficiently opens when ripe and mature in gynoecium and stamen also prematurity, keeps gynoecium all exposed.
Preferably, it collects to obtain the maternal plant by natural mutant.
Preferably, pass through the mutagenic obtained maternal plant of EMS.
More preferably, quinoa seed is impregnated using ethylmethane sulfonate, the quinoa seed handled well is after planting selected into institute
The maternal plant stated.
Preferably, the paternal plant is subjected to by stages repeatedly sowing, makes have enough paternal plant pollen and female parent
The growth of the plant same period.
Preferably, when the maternal plant flower of > 30% is opened, paternal plant is matched with maternal plant, carries out bagging,
Periodically shake plant daily in the maternal plant flowering phase terminates until the florescence.
The present invention is utilized in homophyletic same colored female flower of quinoa prior to male flower maturation, moreover, flower can open dew completely
Female flower out, such target pollen can reach hybridization purpose to gynoecium pollination in advance.It can break through Chenopodiaceae using this species specificity flower
Many and diverse and later period breeding trouble of wheat artificial emasculation, it is only necessary to which mesh can be hybridized with realizing by matching male parent according to breeding objective
Mark, this method is the breakthrough of quinoa breeding technique, is reached quickly, accurately, efficiently.
Compared with prior art the invention has the advantages that：The present invention is planted by the first mature female parent of selection gynoecium
Strain, by reasonable date of seeding, Superior vine selec-tion, pollination solves previous quinoa crossing work large labor intensity in due course, and emasculation is not thorough
With injure gynoecium and cause hybridization failure the problem of, the technology can mass production hybrid seed, have saving of labor's save the cost, operation
It the advantages that simplicity, hybridization success rate is high, economic and environment-friendly, has a extensive future.
Specific embodiment
The technical solution that the present invention will be described in detail is come with specific embodiment below：
Embodiment 1
A kind of two-way cross method of quinoa, includes the following steps：
1）Quinoa maternal plant breeding：Winter in 2017 sows auspicious white Chenopodiaceae No. 8 in Yuanping City's test greenhouse（Complete stool green）There is one plant
Quinoa flower is presented flower and opens, this plant of quinoa is spent the maternal plant as two-way cross prior to the character of stamen maturation by gynoecium.
2）Using and numbering the aubergine quinoa for being JQ-778 as paternal plant is the maternal plant pollination, is produced seeds afterwards
Son, spring in 2018 are experimental field sowed in flat Lu Jiaqi and obtain aubergine F1 plant, it was demonstrated that hybridize successfully.
Embodiment 2
A kind of two-way cross method of quinoa, includes the following steps：
1）Quinoa maternal plant breeding：The ethylmethane sulfonate solution for the use of concentration being 0.8 mol/L, impregnates original quinoa seed
M1It 6 hours, is shaken 6 hours on shaking table, clear water cleans 2 hours, treated seed hole tray seeding and seedling raising, and seedling grows to 8 true leaves
After be transplanted to crop field, this batch processing date is on June 5th, 2017, is transplanted to flat Shandong experimental field June 27, and September harvest on the 10th is planted
Sub- M2, M is sowed in Yuanping City's brooder on March 25th, 20182, Post flowering selects that gynoecium is mature prior to stamen and flower is completely open
Character plant is as hybridization maternal plant.
2）Reasonable date of seeding：To M1During mutagenesis, using planting paternal plant in such a way that the phase is sowed, in this example, choosing
Use number JQ-00077's to sow auspicious quinoa resource as paternal plant.
3）Pollination in due course：When the maternal plant overall 30% or more of plantation blooms completely, by preferred paternal plant and mother
The matching of this plant, carries out artificial pollination, carries out bagging later, periodically shake the plant of pollination daily in flower of female parent open season, directly
Terminate to the florescence.The bag of set was removed in one week after pollination.
4）The F1 seed of harvest hybridization on June 25th, 2018, sowed in warm canopy, and had obtained within August 15th and male parent on June 28
The similar plant of JQ-00077, it was demonstrated that hybridize successfully, material has stability, and hybridizing method is reliable.
The above content is combine specific preferred embodiment to the further description done of the present invention, and it cannot be said that
A specific embodiment of the invention is only limitted to this, for those of ordinary skill in the art to which the present invention belongs, is not taking off
Under the premise of from the present invention, several simple deduction or replace can also be made, all shall be regarded as belonging to the present invention by being submitted
Claims determine scope of patent protection.
Claims (6)
1. a kind of two-way cross method of quinoa, the paternal plant and maternal plant that selection needs to hybridize, pass through artificial pollination
The two-way cross of method progress quinoa；It is characterized in that, the flower for the maternal plant that the two-way cross uses is gynoecium elder generation
In stamen maturation, and when and stamen also prematurity mature in gynoecium, flower sufficiently opens, and keeps gynoecium all exposed.
2. a kind of two-way cross method of quinoa according to claim 1, which is characterized in that collected by natural mutant
Obtain the maternal plant.
3. a kind of two-way cross method of quinoa according to claim 1, which is characterized in that pass through the mutagenic obtained institute of EMS
The maternal plant stated.
4. a kind of two-way cross method of quinoa according to claim 3, which is characterized in that soaked using ethylmethane sulfonate
Quinoa seed is steeped, the quinoa seed handled well is after planting selected to the maternal plant.
5. a kind of two-way cross method of quinoa according to claim 1, which is characterized in that by the paternal plant into
Row repeatedly sowing by stages, makes have enough paternal plant pollen and the maternal plant same period to grow.
6. a kind of two-way cross method of quinoa according to claim 1, which is characterized in that when the maternal plant of > 30%
When flower is opened, paternal plant is matched with maternal plant, carries out bagging, periodically shaken daily in the maternal plant flowering phase
Plant terminates until the florescence.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201811011523.0A CN108901832A (en) | 2018-08-31 | 2018-08-31 | A kind of two-way cross method of quinoa |
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| CN201811011523.0A CN108901832A (en) | 2018-08-31 | 2018-08-31 | A kind of two-way cross method of quinoa |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109644866A (en) * | 2019-02-22 | 2019-04-19 | 山西稼祺农业科技有限公司 | A kind of artificial accurate hybridizing method of quinoa |
| CN110338056A (en) * | 2019-07-08 | 2019-10-18 | 山西省农业科学院生物技术研究中心 | A method of quinoa mutant library is constructed using EMS |
| CN110447534A (en) * | 2019-09-23 | 2019-11-15 | 山东师范大学 | A kind of quinoa mutation construction method based on EMS induction |
| CN110771497A (en) * | 2019-08-22 | 2020-02-11 | 宁夏绿峰源农业科技有限公司 | Quinoa saline-alkali-resistant lodging-resistant breeding method |
| CN116904638A (en) * | 2023-09-08 | 2023-10-20 | 山西稼祺农业科技有限公司 | Kasp markers linked to early females of quinoa and uses thereof |
-
2018
- 2018-08-31 CN CN201811011523.0A patent/CN108901832A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109644866A (en) * | 2019-02-22 | 2019-04-19 | 山西稼祺农业科技有限公司 | A kind of artificial accurate hybridizing method of quinoa |
| CN109644866B (en) * | 2019-02-22 | 2021-05-18 | 山西稼祺农业科技有限公司 | Artificial accurate hybridization method for chenopodium quinoa |
| CN110338056A (en) * | 2019-07-08 | 2019-10-18 | 山西省农业科学院生物技术研究中心 | A method of quinoa mutant library is constructed using EMS |
| CN110338056B (en) * | 2019-07-08 | 2022-07-26 | 山西省农业科学院生物技术研究中心 | Method for constructing quinoa mutant library by using EMS |
| CN110771497A (en) * | 2019-08-22 | 2020-02-11 | 宁夏绿峰源农业科技有限公司 | Quinoa saline-alkali-resistant lodging-resistant breeding method |
| CN110447534A (en) * | 2019-09-23 | 2019-11-15 | 山东师范大学 | A kind of quinoa mutation construction method based on EMS induction |
| CN110447534B (en) * | 2019-09-23 | 2021-05-07 | 山东师范大学 | Quinoa mutant construction method based on EMS induction |
| CN116904638A (en) * | 2023-09-08 | 2023-10-20 | 山西稼祺农业科技有限公司 | Kasp markers linked to early females of quinoa and uses thereof |
| CN116904638B (en) * | 2023-09-08 | 2024-05-07 | 山西稼祺农业科技有限公司 | Kasp markers linked to early females of quinoa and uses thereof |
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Application publication date: 20181130 |