CN102498929A - Method for controlling yellow water exudation of plant bag-cultivated grifola frondosa at culture period - Google Patents
Method for controlling yellow water exudation of plant bag-cultivated grifola frondosa at culture period Download PDFInfo
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- CN102498929A CN102498929A CN2011103073284A CN201110307328A CN102498929A CN 102498929 A CN102498929 A CN 102498929A CN 2011103073284 A CN2011103073284 A CN 2011103073284A CN 201110307328 A CN201110307328 A CN 201110307328A CN 102498929 A CN102498929 A CN 102498929A
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Abstract
The invention discloses a method for controlling yellow water exudation of plant bag-cultivated grifola frondosa at a culture period. The method comprises the following steps of: (1) adjusting the water content of a compost to 62-64 percent according to a weight ratio, bagging with a collar buckling bag, and minimizing an air gap between a collar and the compost; and (2) culturing: inoculating, putting into a culture room, keeping parameters in the culture room constant: the temperature is 22-25 DEG C, the relative humidity is 60-70 percent and the concentration of carbon dioxide is controlled below 2,000 ppm, culturing in dark, controlling to induce the formation of a primordium within 20 days at the latter ripening stage of bagful hyphae. Before the induction of the primordium formation for the grifola frondosa, i.e., at the culturing and latter ripening stages of the grifola frondosa, the physicochemical properties of the compost are improved, the culture environment parameters are kept constant in a certain range, the production of metabolism yellow water can be reduced remarkably or avoided, the formation rate of the primordium is increased, pollution is lowered, and the mushroom yield and quality are improved.
Description
Technical field
The present invention relates to the control method that a kind of grifola frondosus cultivation culture period is told yellow water, is to disclose a kind of batch production bag to plant the control method that the grifola frondosus culture period is told yellow water specifically.
Background technology
Grifola frondosus [
Grifola frondosa (Dicks, ex Fr) S.F.Gray], having another name called polyporus frondosus, thousand Buddhist bacterium, chestnut mushroom, cloud gill fungus, lotus flower bacterium etc., it is fine and soft that Japan is referred to as dance.The grifola frondosus meat is tender and crisp, and is nutritious, contains bioactivator, and in Japan, grifola frondosus is used to treat cancer of the stomach, cancer of the esophagus, breast cancer.Its fruit body, mycelial extract tool active anticancer can obviously improve chemotherapy, the caused immunologic function reduction of radiotherapy.
Telling yellow water is meant: the mycelium of edible fungus species cultivation bag between culture period, the yellow liquid that occurs in the medium thing.Edible fungus species often after the mycelia atrophy, yellow liquid occurs under bad condition.Telling the yellow water most cases is that hypha of edible fungus is old and feeble, hyphal cell hydrolysis self-dissolving, and protoplasm overflows, and is the concrete manifestation of mycelial growth worsening condition, the aging decline of thalline.Grifola frondosus is because extremely responsive to composts or fertilisers of cultivating and condition of culture, and its resistance is poor, in case environmental inadequacies or fluctuate bigger than normally, mycelia is output metabolism yellow water very easily just, shows mycelia aging or degradation phenomena, causes cultivation bag pollution or original hase to form difficulty.This also is that to have only some areas at home be the cultivation of seasonal family workshop type, and does not occur the major reason of industrial and annual cultivation grifola frondosus as yet.
Domestic edible fungus industrial production at present is in the fast-developing phase, but the grifola frondosus factory culture still belongs to blank, for this reason; Find out and effectively control the parameter of each item environmental factor, the particularly incubation of grifola frondosus cultivation process; And keep constant, control produces the factor of metabolism yellow water, reduces and pollutes; Improving the original hase formation rate, is the important breakthrough that realizes China's grifola frondosus factory culture.
Summary of the invention
The objective of the invention is to solve the technological deficiency that grifola frondosus cultivation culture period is prone to tell yellow water in the prior art; Provide a kind of batch production bag to plant the control method that the grifola frondosus culture period is told yellow water; Through the adjustment of composts or fertilisers of cultivating physicochemical property, change pack mode,, effectively control appearance " telling yellow water " phenomenon in the incubation to each envirment factor quantification of whole culture period, constantization; Reduce grifola frondosus and pollute, improve the yield rate of grifola frondosus cultivation.
The present invention is achieved in that a kind of batch production bag plants the control method that the grifola frondosus culture period is told yellow water; Comprise Mixed culture material siccative, add water adjusting composts or fertilisers of cultivating water content; It is characterized in that: comprise the steps: 1) with the water content of composts or fertilisers of cultivating by weight being adjusted to 62 ~ 64%; Adopt the packed bag of cover latch closure then, the space between the control collar and the composts or fertilisers of cultivating makes the space reach minimum; 2) cultivate: put into culturing room after the inoculation; It is constant that the culturing room intrinsic parameter keeps: temperature is that 22 ~ 25 ℃, relative moisture are 60 ~ 70%, gas concentration lwevel is controlled at below the 2000ppm, dark culturing, and the mycelia latter stage of ripening of sending out purseful is controlled at and formed with the interior original hase of inducing in 20 days.
Culture bag keeps static in the incubation of said step 2, avoids that culture bag is carried out machinery and moves.
Contain weight ratio in the said Mixed culture siccative and be 20 ~ 30% band shell millet and 1.0 ~ 1.5% water-loss reducer.
When inoculating in the said step 2, insert 20 ~ 30ml grifola frondosus liquid strain in every bag of composts or fertilisers of cultivating.
The invention has the beneficial effects as follows: before inducing the grifola frondosus original hase to form; Be that grifola frondosus is cultivated and latter stage of ripening, constant through control culture period envirment factor, the improvement of composts or fertilisers of cultivating physicochemical property; Can effectively control late stage of culture and produce the metabolism yellow water; Improve the original hase formation rate, reduce and pollute, guarantee the regularity and the yield and quality of fruiting.
Embodiment
A kind of batch production bag of the present invention is planted the grifola frondosus culture period and is told the control method of yellow water and comprise the steps: 1) Mixed culture material siccative; And add water with the water content of composts or fertilisers of cultivating by weight being adjusted to 62 ~ 64%; Adopt the packed bag of cover latch closure then; Space between the control collar and the composts or fertilisers of cultivating makes the space reach minimum; 2) cultivate: insert grifola frondosus liquid strain 20 ~ 30ml inoculation with every bag of composts or fertilisers of cultivating; Put into culturing room after the inoculation; It is constant that the culturing room intrinsic parameter keeps: temperature is that 22 ~ 25 ℃, relative moisture are 60 ~ 70%, gas concentration lwevel is controlled at below the 2000ppm, dark culturing, and the mycelia latter stage of ripening of sending out purseful is controlled at and formed with the interior original hase of inducing in 20 days.Culture bag keeps static in the incubation, avoids that culture bag is carried out machinery and moves.
Contain weight ratio in the said Mixed culture siccative and be 20 ~ 30% band shell millet and 1.0 ~ 1.5% water-loss reducer.
Conventional Grifola frondosa culture material siccative mainly is made up of wood chip, wheat bran, corn flour, gypsum, earth, and the present invention is on the basis of conventional composts or fertilisers of cultivating, and the culture environment of grifola frondosus is improved.
With regard to specific embodiment the present invention is done further elaboration below.
Embodiment one: 1) in conventional composts or fertilisers of cultivating siccative by weight adding 20% band shell millet and 1.5% water-loss reducer; Mix and spice; Regulate composts or fertilisers of cultivating water content to 62% by weight adding entry; With the packed bag of cover latch closure, guarantee that pack back composts or fertilisers of cultivating is minimum with the space of cover interannular then, sterilization is cooled to 23 ℃; 2) cultivate: in culture bag, inserting and being placed on temperature behind the 20ml grifola frondosus liquid strain is 22 ℃, and relative moisture is 60%, and gas concentration lwevel is 1800ppm, dark culturing, and the mycelia latter stage of ripening of sending out purseful is 10 days, before inducing original hase to form, avoids machinery to move.And guarantee that above-mentioned condition of culture keeps constant before inducing original hase to form.
Embodiment two: 1) the composts or fertilisers of cultivating siccative is mixed, and add water and be adjusted to 63% by weight the water content with composts or fertilisers of cultivating, then with the packed bag of cover latch closure, guarantee that pack back composts or fertilisers of cultivating is minimum with the space of cover interannular, sterilization is cooled to 24 ℃; 2) cultivate: in culture bag, inserting and being placed on temperature behind the 25ml liquid strain is 23 ℃, and relative moisture is 65%, and gas concentration lwevel is 1700ppm, dark culturing, and the mycelia latter stage of ripening of sending out purseful is 15 days, before inducing original hase to form, avoids machinery to move.And guarantee that above-mentioned condition of culture keeps constant before inducing original hase to form.
Embodiment three: 1) in the composts or fertilisers of cultivating siccative by weight adding 25% band shell millet and 1% water-loss reducer, mix and stir, regulate composts or fertilisers of cultivating water content to 64% by weight adding entry; With the packed bag of cover latch closure, guarantee that pack back composts or fertilisers of cultivating is minimum with the space of cover interannular then, sterilization is cooled to 25 ℃; Being placed on temperature behind the access 25ml liquid strain is 24 ℃; Relative moisture is 70%, and gas concentration lwevel is 1850ppm, dark culturing; The mycelia latter stage of ripening of sending out purseful is 15 days, before inducing original hase to form, avoids machinery to move.And guarantee that above-mentioned condition of culture keeps constant before inducing original hase to form.
Embodiment four: 1) earth in the conventional composts or fertilisers of cultivating siccative is removed, the use weight ratio is 30% band shell millet replacement, and adds 1.2% water-loss reducer; Carrying out spice, packing the back water content is 64%, guarantees that the space of pack back composts or fertilisers of cultivating and cover interannular is minimum, and sterilization is cooled to 23 ℃; Being placed on temperature behind the access 30ml liquid strain is 24 ℃; Relative moisture is 68%, and gas concentration lwevel is 1600ppm, dark culturing; The mycelia latter stage of ripening of sending out purseful is 20 days, before inducing original hase to form, avoids machinery to move.And guarantee that above-mentioned condition of culture keeps constant before inducing original hase to form.
Embodiment five: 1), adds water by weight regulating composts or fertilisers of cultivating water content to 63%, then with the packed bag of cover latch closure with composts or fertilisers of cultivating siccative mixing stirring; Composts or fertilisers of cultivating is minimum with the space of cover interannular after guaranteeing pack, and sterilization is cooled to 24 ℃, and being placed on temperature behind the access 20ml liquid strain is 22 ℃; Relative moisture is 70%, and gas concentration lwevel is 1600ppm, dark culturing; The mycelia latter stage of ripening of sending out purseful is 20 days, before inducing original hase to form, avoids machinery to move.And guarantee that above-mentioned condition of culture keeps constant before inducing original hase to form.
Following table is the comparison of the embodiment of the invention and prior art Comparative Examples:
Can find out from last table; Contain weight ratio in the Mixed culture siccative and be 20 ~ 30% band shell millet and 1.0 ~ 1.5% water-loss reducer; Put into culturing room after the inoculation, it is constant that the culturing room intrinsic parameter keeps: temperature is that 22 ~ 25 ℃, relative moisture are 60 ~ 70%, gas concentration lwevel is controlled at below the 2000ppm, dark culturing, and the mycelia latter stage of ripening of sending out purseful is controlled at and formed with the interior original hase of inducing in 20 days; Culture bag keeps static in the incubation, avoids that culture bag is carried out machinery and moves.Can obviously reduce metabolism yellow water amount, reduce pollution rate, improve the grifola frondosus yield rate.
Claims (5)
1. a batch production bag is planted the control method that the grifola frondosus culture period is told yellow water; Comprise Mixed culture material siccative, add water adjusting composts or fertilisers of cultivating water content; It is characterized in that: comprise the steps: 1) with the water content of composts or fertilisers of cultivating by weight being adjusted to 62 ~ 64%; Adopt the packed bag of cover latch closure then, the space between the control collar and the composts or fertilisers of cultivating makes the space reach minimum; 2) cultivate: put into culturing room after the inoculation, it is constant that culturing room's intrinsic parameter keeps: temperature is that 22 ~ 25 ℃, relative moisture are 60 ~ 70%, gas concentration lwevel is controlled at below the 2000ppm, dark culturing.
2. plant the control method that the grifola frondosus culture period is told yellow water according to the described a kind of batch production bag of claim 1, it is characterized in that: culture bag keeps static in the incubation of said step 2, avoids that culture bag is carried out machinery and moves.
3. plant the control method that the grifola frondosus culture period is told yellow water according to the described a kind of batch production bag of claim 1, it is characterized in that: when inoculating in the said step 2, insert 20 ~ 30ml grifola frondosus liquid strain in every bag of composts or fertilisers of cultivating.
4. plant the control method that the grifola frondosus culture period is told yellow water according to the described a kind of batch production bag of claim 1, it is characterized in that: the mycelia latter stage of ripening of sending out purseful, is controlled at and formed with the interior original hase of inducing in 20 days.
5. plant the control method that the grifola frondosus culture period is told yellow water according to the described a kind of batch production bag of claim 1, it is characterized in that: contain weight ratio in the said Mixed culture siccative and be 20 ~ 30% band shell millet and 1.0 ~ 1.5% water-loss reducer.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106797805A (en) * | 2017-03-17 | 2017-06-06 | 北京市农业技术推广站 | A kind of chestnut mushroom exempts from soil fruiting cultivation technique |
| CN107242028A (en) * | 2017-07-26 | 2017-10-13 | 上海光明森源生物科技有限公司 | The method that positioning mycelium stimulation induces fruiting in grifola frondosus factorial praluction bag |
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| CN1082808A (en) * | 1993-06-30 | 1994-03-02 | 迁西县实用菌研究所 | Method for bionic cultivation of Huishuhua mushroom |
| CN101715696A (en) * | 2009-12-18 | 2010-06-02 | 上海市农业科学院 | Factory cultivation method of maitake |
| CN101731097A (en) * | 2009-12-18 | 2010-06-16 | 上海市农业科学院 | Grifola frondosus liquid strain and method for cultivating grifola frondosus by using liquid strain |
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2011
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1082808A (en) * | 1993-06-30 | 1994-03-02 | 迁西县实用菌研究所 | Method for bionic cultivation of Huishuhua mushroom |
| CN101715696A (en) * | 2009-12-18 | 2010-06-02 | 上海市农业科学院 | Factory cultivation method of maitake |
| CN101731097A (en) * | 2009-12-18 | 2010-06-16 | 上海市农业科学院 | Grifola frondosus liquid strain and method for cultivating grifola frondosus by using liquid strain |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106797805A (en) * | 2017-03-17 | 2017-06-06 | 北京市农业技术推广站 | A kind of chestnut mushroom exempts from soil fruiting cultivation technique |
| CN107242028A (en) * | 2017-07-26 | 2017-10-13 | 上海光明森源生物科技有限公司 | The method that positioning mycelium stimulation induces fruiting in grifola frondosus factorial praluction bag |
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Application publication date: 20120620 |