CN102498793B - Method for removing endophytes from pistacia chinensis bunge seeds and quickly germinating pistacia chinensis bunge seeds - Google Patents
Method for removing endophytes from pistacia chinensis bunge seeds and quickly germinating pistacia chinensis bunge seeds Download PDFInfo
- Publication number
- CN102498793B CN102498793B CN 201110323130 CN201110323130A CN102498793B CN 102498793 B CN102498793 B CN 102498793B CN 201110323130 CN201110323130 CN 201110323130 CN 201110323130 A CN201110323130 A CN 201110323130A CN 102498793 B CN102498793 B CN 102498793B
- Authority
- CN
- China
- Prior art keywords
- seeds
- seed
- chinensis bunge
- pistacia chinensis
- germination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a method for removing endophytes from pistacia chinensis bunge seeds and quickly germinating the pistacia chinensis bunge seeds. The method mainly comprises the following steps of: sterilizing the pistacia chinensis bunge seeds, of which the shells are removed by using ethanol and sodium hypochlorite; soaking the seeds in water to perform ultrasonic processing; and flushing the seeds, and then inoculating the seeds to a seed germination culture medium to obtain aseptic robust seedlings. By adoption of the method, the difficulty that the germination rate of the seeds is influenced by pollution caused by inexhaustive sterilization and long sterilization time is solved, and more importantly, the germination rate of the seeds is increased, the seedlings grow robustly, and a basis is provided for tissue culture and genetic transformation of pistacia chinensis bunge.
Description
Technical field
The present invention relates to biotechnology and modern agricultural technology field, be specifically related to a kind of method of Chinese pistache seed endophyte removal and fast-germination.
Background technology
chinese pistache (
pistacia chinensisbunge) be a kind of important perennial woody oil plants, have a very wide distribution, can be in waste mountain waste and sand ground plantation, suitable large tracts of land afforestation, and the seed oil content is up to more than 40%, by the biodiesel of Chinese pistache seed production, being clean renewable energy resources, is the petroleum diesel substitute of high-quality, has been confirmed as following eco-energy woods construction and biodiesel industry and has first developed seeds.Because it is used as the promise well of Tree Species as Bio-energy, at present more and more for the research of Chinese pistache, but be substantially all to concentrate on the aspect such as culture technique, and less for the research of its tissue cultivation and genetic transformation aspect; At present, country builds more and more and payes attention to for development bioenergy woods, and carrying out genetic improvement by Molecular tools is a kind of mode faster.Yet there are the problems such as seed disinfection is difficult for thoroughly, rudiment is slow, germination rate is low in Chinese pistache, therefore need to a kind ofly can remove preferably its pollution and can improve the method for germination rate again.By using general disinfection way effect, be very undesirable, a kind of therefore develop low pollution, high germination rate method seems extremely urgent more.
Summary of the invention
The acquisition of Chinese pistache aseptic explant is the basis of organizing cultivation and Study on Genetic Transformation.Yet, because the Chinese pistache seed disinfection is difficult for thoroughly, particularly storage time length or plum rain season are very difficult especially, have had a strong impact on its research progress.Therefore, invent that a kind of not only Disinfection Effect is good but also germination rate is high, the more healthy and stronger method of the aseptic seedling of acquisition is key character of the present invention.
The object of the invention is to for the undesirable problem of the common Disinfection Effect of Chinese pistache, design that a kind of not only Disinfection Effect is good but also aseptic seedling germination rate that obtain is high, the method for robust growth.
The present invention adopts following technical scheme to achieve these goals:
A kind of method of Chinese pistache seed endophyte removal and fast-germination, it is characterized in that: the Chinese pistache seed that will remove shell is disinfected through ethanol and clorox, then seed is immersed in water and carries out ultrasonic processing, be seeded in after rinsing well on the seed germination medium, obtain the seedling of aseptic stalwartness.
The method of described Chinese pistache endophyte removal and fast-germination, it is characterized in that: described ethanol and clorox are disinfected and are referred to the Ethanol Treatment 1 minute of seed first being used to 65-75%, aseptic water washing once, is then processed 10 minutes with the clorox of 15-25%.
The method of described Chinese pistache endophyte removal and fast-germination, it is characterized in that: described ultrasonic processing refers to seed is immersed in sterile water, then be placed in the ultrasonic instrument that temperature is arranged on 18-22 ℃ and process 8-12 minute, sterile water wash is clean afterwards.
The method of described Chinese pistache endophyte removal and fast-germination, it is characterized in that: described germination medium refers to: 1/2MS+6-BA2.0 mg/L+SNP15 mg/L+ glucose 20g/L+ agar 8 g/L, pH is adjusted to 5.8-6.0.
Beneficial effect of the present invention:
The present invention processes common sterilization to combine with ultrasonic cleverly, by the synergy between them, has significantly reduced the pollution rate of seed endophyte, has improved the germination rate of seed simultaneously; The clean seed of sterilization is seeded on defined medium, has obtained the aseptic seedling of robust growth.
Embodiment
embodiment 1:the concrete operation method of the removal of Chinese pistache seed endophyte and fast-germination is as follows:
1, at first the Chinese pistache seed that will remove shell cleans several times with pure water, then uses 70% Ethanol Treatment 1 minute, aseptic water washing once, then with 20% clorox processing 10 minutes, during ceaselessly shake, aseptic water washing 3-4 time;
2, the seed through rinsing well is immersed in aseptic conical flask, puts into 20 ℃ of ultrasonic processing of ultrasonic instrument 10 minutes, and aseptic water washing is to clean afterwards;
3, the clean seed of sterilizing is seeded in germination medium 1/2MS+6-BA2.0 mg/L+SNP15 mg/L+ glucose 20g/L+ agar 8 g/L, within about one week, can obtain without endophytic bacterial contamination, germination rate is high and grow sturdy aseptic seedling.
Claims (2)
1. a Chinese pistache seed endophyte is removed and the method for fast-germination, it is characterized in that: the Chinese pistache seed that will remove shell is disinfected through ethanol and clorox, then seed is immersed in water and carries out ultrasonic processing, be seeded in after rinsing well on the seed germination medium, obtain the seedling of aseptic stalwartness; Described ethanol and clorox are disinfected and are referred to the Ethanol Treatment 1 minute of seed first being used to 65-75%, and aseptic water washing once, is then processed 10 minutes with the clorox of 15-25%; Described ultrasonic processing refers to seed is immersed in sterile water, then is placed in the ultrasonic instrument that temperature is arranged on 18-22 ℃ and processes 8-12 minute, and sterile water wash is clean afterwards.
2. Chinese pistache endophyte according to claim 1 is removed and the method for fast-germination, and it is characterized in that: described germination medium refers to: 1/2MS+6-BA2.0 mg/L+SNP15 mg/L+ glucose 20g/L+ agar 8 g/L, pH is adjusted to 5.8-6.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110323130 CN102498793B (en) | 2011-10-22 | 2011-10-22 | Method for removing endophytes from pistacia chinensis bunge seeds and quickly germinating pistacia chinensis bunge seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110323130 CN102498793B (en) | 2011-10-22 | 2011-10-22 | Method for removing endophytes from pistacia chinensis bunge seeds and quickly germinating pistacia chinensis bunge seeds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102498793A CN102498793A (en) | 2012-06-20 |
| CN102498793B true CN102498793B (en) | 2013-12-18 |
Family
ID=46210976
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110323130 Active CN102498793B (en) | 2011-10-22 | 2011-10-22 | Method for removing endophytes from pistacia chinensis bunge seeds and quickly germinating pistacia chinensis bunge seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102498793B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102907324B (en) * | 2012-11-06 | 2014-02-05 | 河南科技大学 | Method for rapidly and efficiently rooting pistacia chinensis bunge by taking stem tips and stem segments as explants |
| CN104472064A (en) * | 2014-11-17 | 2015-04-01 | 柳州市天姿园艺有限公司 | Method for accelerating germination of Brazilian cherry seed before sowing |
| CN105123750A (en) * | 2015-10-15 | 2015-12-09 | 贵州省生物技术研究所 | Mold bacteriostatic agent for open culture of potato tissue culture seedlings and using method thereof |
| CN105706571A (en) * | 2016-03-07 | 2016-06-29 | 兰州大学 | Method for eliminating seed-borne grass endophytic fungi |
| CN107022510A (en) * | 2017-06-06 | 2017-08-08 | 昆明学院 | A kind of separation method of endophyte of plant |
| CN108781818A (en) * | 2018-02-06 | 2018-11-13 | 安徽新安古建园林建设股份有限公司 | A kind of high efficiency seedling cultivating method of Chinese pistache nursery stock |
| CN110915356A (en) * | 2019-12-06 | 2020-03-27 | 怀化学院 | Germination promoting method for plant seeds |
| CN114303515A (en) * | 2021-12-23 | 2022-04-12 | 昆明学院 | Method for removing endophytes from corn seeds and rapidly germinating corn seeds |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001122728A (en) * | 1999-10-20 | 2001-05-08 | Shiseido Co Ltd | Protease inhibitor |
| CN101933437A (en) * | 2010-07-27 | 2011-01-05 | 中国科学院等离子体物理研究所 | Chinese pistache nondestructive embryo-stripping non-dormancy sprouting method |
| WO2011028601A2 (en) * | 2009-08-26 | 2011-03-10 | Mary Kay Inc. | Topical skin care formulations comprising plant extracts |
| CN102144457A (en) * | 2010-02-04 | 2011-08-10 | 李洁 | Cultivation method of pistacia chinensis bunge |
-
2011
- 2011-10-22 CN CN 201110323130 patent/CN102498793B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001122728A (en) * | 1999-10-20 | 2001-05-08 | Shiseido Co Ltd | Protease inhibitor |
| WO2011028601A2 (en) * | 2009-08-26 | 2011-03-10 | Mary Kay Inc. | Topical skin care formulations comprising plant extracts |
| CN102144457A (en) * | 2010-02-04 | 2011-08-10 | 李洁 | Cultivation method of pistacia chinensis bunge |
| CN101933437A (en) * | 2010-07-27 | 2011-01-05 | 中国科学院等离子体物理研究所 | Chinese pistache nondestructive embryo-stripping non-dormancy sprouting method |
Non-Patent Citations (5)
| Title |
|---|
| 刘洋.阿月浑子和黄连木外植体启动培养研究.《中国优秀博硕士学位论文全文数据库（硕士）农业科技辑》.2005,(第4期),第47页. * |
| 周会玲等.超声波清洗对鲜切豆角贮藏品质的影响.《食品研究与开发》.2010,第31卷(第9期),第191-193页. |
| 超声波消毒研究进展;陈荣凤等;《上海预防医学杂志》;19991130;第11卷(第11期);第492页 * |
| 超声波清洗对鲜切豆角贮藏品质的影响;周会玲等;《食品研究与开发》;20100930;第31卷(第9期);第191-193页 * |
| 陈荣凤等.超声波消毒研究进展.《上海预防医学杂志》.1999,第11卷(第11期),第492页. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102498793A (en) | 2012-06-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102498793B (en) | Method for removing endophytes from pistacia chinensis bunge seeds and quickly germinating pistacia chinensis bunge seeds | |
| CN102150624B (en) | Tissue culture and rapid propagation method for pinellia tuber plant | |
| CN101926287B (en) | Method for culturing tissue of 'Zhongzhen No.1' | |
| CN103858762A (en) | Rapid proliferation method for anoectochilus formosanus tissue culture | |
| CN102783415B (en) | Method for conservation in vitro of cassava germplasm resources with stability and high efficiency | |
| US11499134B2 (en) | Method for culturing ginseng cell with high content of ginsenoside | |
| CN108834894B (en) | Tissue culture method of uncaria | |
| CN103583357B (en) | Method for sterile seeding of lithops and establishing regeneration system | |
| CN102919131B (en) | Tissue cultivation method of soybean | |
| CN104255499A (en) | Diospyros rhombifolia tissue culture and immediate propagation method | |
| CN1742563A (en) | Method for fast breeding water caltrop seedlings | |
| CN110122334B (en) | Special culture medium for rapid propagation of arundo donax linn and culture method thereof | |
| CN101816287B (en) | Method for culturing Iris pseudacorus callus | |
| CN106069754A (en) | A kind of Arundo donax tissue culture special culture media and cultural method | |
| KR101047306B1 (en) | Somatic Embryogenesis of Lily Trees | |
| CN101743907B (en) | Rhizoma curculiginis breeding technique | |
| CN100463960C (en) | Separation technology of orchidaceal plant mycorrhizal fungi monobyssisede | |
| CN109601392A (en) | A kind of Huperzia serrata excised cotyledon, breeding and preservation method | |
| CN105409773A (en) | Lophophora williamsii sterile seeding and regeneration system establishing method | |
| CN109380083A (en) | A kind of tissue culture technique of bicolor | |
| CN106962189A (en) | A kind of method for promoting Snakegourd Fruit tissue culture to break up | |
| CN103004605B (en) | Culture technique for aseptic pepper seedlings | |
| CN102301959A (en) | Method for regenerating Chinese pistache plant by taking cotyledons as explants | |
| CN102893864B (en) | Rhus chinensis adventitious bud high frequency plant regeneration method | |
| CN108668899B (en) | Method for improving induction rate of masson pine somatic embryos |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |