CN101933437A - Chinese pistache nondestructive embryo-stripping non-dormancy sprouting method - Google Patents
Chinese pistache nondestructive embryo-stripping non-dormancy sprouting method Download PDFInfo
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- CN101933437A CN101933437A CN 201010242900 CN201010242900A CN101933437A CN 101933437 A CN101933437 A CN 101933437A CN 201010242900 CN201010242900 CN 201010242900 CN 201010242900 A CN201010242900 A CN 201010242900A CN 101933437 A CN101933437 A CN 101933437A
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Abstract
The invention discloses a Chinese pistache nondestructive embryo-stripping non-dormancy sprouting method which comprises the following steps of: cutting a notch on the edge of the immature or mature seed of Chinese pistache, with the size of the notch causing no damage to an embryo as a standard, then transversely applying pressure to the seed to form a gap at the notch, lightly stripping a seed shell from the gap to obtain a complete Chinese pistache seed embryo, directly sprouting the Chinese pistache seed without a method for breaking dormancy through low-temperature wet (sand) storage lamination or gibberellin GA, rare-earth processing and the like, and growing into a plant. The method for nondestructively stripping the Chinese pistache seed shell has good embryo-stripping effect and high effect, and 100-150 grains of embryos can be stripped per hour by each person.
Description
Technical field
The present invention relates to biological technical field, specifically is that the harmless stripping of a kind of Chinese pistache embryo does not have dormancy and sends out seedling-growing method.
Background technology
Chinese pistache is the woody energy-source plant that China has application prospect at present most, 24 provinces (autonomous region, municipality directly under the Central Government) on the south China Hebei all have wild and cultivation, seed oil content is up to 42%, biodiesel with Chinese pistache production is the renewable energy resources of cleaning, is the petroleum diesel substitute of high-quality.
Be the harvesting and the harvest time of Chinese pistache seed annual October to November, and the seed shelf life of harvesting is generally 1 year, surpasses after 1 year, and the quality of seed seriously descends, and germination rate is extremely low.And just there has been resting stage in the Chinese pistache seed of results, can not directly germinate, and must hide through the low temperature about 100 days (4 ℃) wet (sand) and handle and could sprout.This not only loses time, also need simultaneously cryogenic conditions, need the cost manpower and financial resources, the more important thing is, this makes the time of seed sprouting be confined to a special time period, making manpower financial capacity's use undue concentration, particularly is that the purpose project implementation lacks flexibility with the scientific research, needs the time of several months.Therefore, be badly in need of that exploitation is a kind of can break the seed dormant technology of Chinese pistache, make its quick germination.
Seed dormancy is often referred to has blodynamic seed is not sprouted (germination) under suitable sprouting condition phenomenon.Mechanism according to dormancy can be divided into the seed dormancy type physics dormancy, chemical dormancy, physiological dormancy etc.; Degree according to dormancy can be divided into shallow dormancy, moderate dormancy and deep dormancy etc. again; Can be divided into again according to the origin of dormancy reason and to force dormancy (condition dormancy) and body dormancy (primary dormancy); And dormancy can be divided into external source dormancy (planting the shell dormancy) and Inner source dormancy (embryo dormancy) according to the anatomical position of diapause factor in seed.Different seed dormant mechanism differences, according to the mechanism of dormancy, seed dormancy can be broken with the method for various physics or chemistry, if after dormancy belonged to kind of a shell dormancy and then removes kind of shell, seed can germinate.
Summary of the invention
The invention provides the harmless stripping of a kind of Chinese pistache embryo does not have dormancy and sends out seedling-growing method, with the harmless kind shell of removing Chinese pistache prematurity or mature seed of artificial method, obtain complete seed embryo, can not need the method for low warm and humid (sand) Tibetan lamination or breaking dormancies such as gibberellin GA, rare earth processing and directly make the Chinese pistache seed sprouting, grow up to plant.
Technical scheme of the present invention is:
The harmless stripping of Chinese pistache embryo does not have dormancy and sends out seedling-growing method, it is characterized in that: be edge scissor one breach Chinese pistache prematurity or mature seed, the size of breach is a standard not hurt embryo, then seed is laterally exerted pressure, make indentation, there that the slit be arranged, from slit strip off kind gently shell, obtain complete Chinese pistache seed embryo, described Chinese pistache seed embryo through sterilization or without sterilization in the container of water is arranged or be applicable to germinate on the medium that plant cultivates and obtain the Chinese pistache seedling.
The harmless stripping of described Chinese pistache embryo does not have dormancy and sends out seedling-growing method, it is characterized in that: described sterilization is to be in the ethanol of 65-75% immersion treatment 1-2 minute with Chinese pistache seed embryo at mass fraction, is mercuric chloride immersion treatment 12-17 minute of 0.1-0.2% then with mass fraction.
Beneficial effect of the present invention is:
1, Chinese pistache kind subshell is hard, there is wax on the surface, obtain very difficulty of complete injury-free embryo, be to utilize embryo to germinate and the major obstacles of test such as tissue culture, do not see at present the report that obtains and utilize harmless Chinese pistache embryo to test is arranged, the harmless stripping of Chinese pistache embryo technology will provide the important techniques support for the research and development of the first-selected woody energy-source plant Chinese pistache of China, be in almost blank situation for a long time with the related basic study of breaking this plant that important application prospect is arranged;
2, the present invention confirms that the Chinese pistache Seed Dormancy is mainly caused by kind of a shell dormancy, remove kind of a shell and can break its resting stage, thereby broken the situation that in the past must germinate, can germinate and organize the training experiment at any time through the process ability breaking dormancy that low warm and humid (sand) of time-consuming, effort, consumption wealth is hidden.
Embodiment
The harmless stripping of Chinese pistache embryo does not have dormancy and sends out seedling-growing method:
Chinese pistache prematurity or mature seed is first at Chinese pistache seed edge scissor one small gap with instruments such as sharp mouth vices, and the size of breach is a standard not hurt embryo; With instruments such as tweezers seed is laterally exerted pressure then, make indentation, there that the slit be arranged, and with instrument or nail insertion breach such as sharp tweezers, the shell of strip off kind gently promptly can obtain complete embryo.Everyone per hour peelable embryo 100-150 grain.
The complete embryo that obtains was handled 1 minute and 15 seconds with 70% alcohol immersion, use 0.1% mercuric chloride immersion treatment 15 minutes again, in sterile petri dish, germinate then, can there be the root of 1-2CM to grow in three days, can become seedling in 7-10 days, germination rate can reach about 50%, quite or be higher than and hide lamination through suitable wet (sand) and handle seed germination rate.
Claims (2)
1. the harmless stripping of Chinese pistache embryo does not have dormancy and sends out seedling-growing method, it is characterized in that: be edge scissor one breach Chinese pistache prematurity or mature seed, the size of breach is a standard not hurt embryo, then seed is laterally exerted pressure, make indentation, there that the slit be arranged, from slit strip off kind gently shell, obtain complete Chinese pistache seed embryo, described Chinese pistache seed embryo through sterilization or without sterilization in the container of water is arranged or be applicable to germinate on the medium that plant cultivates and obtain the Chinese pistache seedling.
2. the harmless stripping of Chinese pistache according to claim 1 embryo does not have dormancy and sends out seedling-growing method, it is characterized in that: described sterilization is to be in the ethanol of 65-75% immersion treatment 1-2 minute with Chinese pistache seed embryo at mass fraction, is mercuric chloride immersion treatment 12-17 minute of 0.1-0.2% then with mass fraction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010242900 CN101933437A (en) | 2010-07-27 | 2010-07-27 | Chinese pistache nondestructive embryo-stripping non-dormancy sprouting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010242900 CN101933437A (en) | 2010-07-27 | 2010-07-27 | Chinese pistache nondestructive embryo-stripping non-dormancy sprouting method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101933437A true CN101933437A (en) | 2011-01-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010242900 Pending CN101933437A (en) | 2010-07-27 | 2010-07-27 | Chinese pistache nondestructive embryo-stripping non-dormancy sprouting method |
Country Status (1)
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| CN (1) | CN101933437A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498793A (en) * | 2011-10-22 | 2012-06-20 | 中国科学院合肥物质科学研究院 | Method for removing endophytes from pistacia chinensis bunge seeds and quickly germinating pistacia chinensis bunge seeds |
| CN105875315A (en) * | 2014-12-24 | 2016-08-24 | 山东农业大学 | Protected non-dormancy peach cultivation method |
-
2010
- 2010-07-27 CN CN 201010242900 patent/CN101933437A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 《林业科技通讯》 19890228 邵蓓蓓 层积和变温对十种林木种子萌发的影响 第4-7页 1-2 , 第2期 2 * |
| 《果树科学》 19960831 陶俊等 桃种子的休眠与萌发研究-种皮的调控作用 第233-236页 1-2 第13卷, 第4期 2 * |
| 《西北植物学报》 20030531 杨期和等 职务种子休眠的原因及休眠的多样性 第837-843页 1-2 第23卷, 第5期 2 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498793A (en) * | 2011-10-22 | 2012-06-20 | 中国科学院合肥物质科学研究院 | Method for removing endophytes from pistacia chinensis bunge seeds and quickly germinating pistacia chinensis bunge seeds |
| CN102498793B (en) * | 2011-10-22 | 2013-12-18 | 中国科学院合肥物质科学研究院 | Method for removing endophytes from pistacia chinensis bunge seeds and quickly germinating pistacia chinensis bunge seeds |
| CN105875315A (en) * | 2014-12-24 | 2016-08-24 | 山东农业大学 | Protected non-dormancy peach cultivation method |
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Application publication date: 20110105 |