CN104261972A - Method for promoting water chestnut seeds to sprout and used culture medium - Google Patents
Method for promoting water chestnut seeds to sprout and used culture medium Download PDFInfo
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- CN104261972A CN104261972A CN201410457519.2A CN201410457519A CN104261972A CN 104261972 A CN104261972 A CN 104261972A CN 201410457519 A CN201410457519 A CN 201410457519A CN 104261972 A CN104261972 A CN 104261972A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention relates to a method for promoting plant seeds to sprout, and in particular relates to a method for promoting water chestnut seeds to sprout and a used culture medium, belonging to the technical field of biology. The method for promoting the plant seeds to sprout disclosed by the invention comprises the following steps of: immersing, sterilizing and cleaning water chestnut seed teguments after peeling, transferring the obtained seeds on the culture medium, and culturing by illuminating for 8-12h every day under the illumination conditions of 26-30 DEG C and 8000-10000 Lx, wherein the culture cycle is 15-25 d. According to the invention, after the water chestnut seed teguments are peeled and removed, on one hand, the sprouting inhibition effect caused by the teguments can be reduced; on the other hand, the chestnut seeds can absorb moisture rapidly, so that the sprouting requirements are achieved; after the seeds are immersed by warm water, the seed sprouting inhibitors can be obviously reduced; the seeds can sprout easily; furthermore, due to hormone of the culture medium and nutrition supply, the seeds can be promoted to sprout; the survival rate of sprouted seeds is increased; the seed sprouting rate is above 60%; and the survival rate of the sprouted seeds achieves 100%.
Description
Technical field
The present invention relates to a kind of method promoting plant seed germination, particularly relate to the method and used medium that promote water chestnut seed germination, belong to biological technical field.
Background technology
Seed is the distinctive propagulum of plant.But since several thousand, water chestnut is all adopt bulb to breed, and this modes of reproduction is in fact a kind of vegetative propagation, and long-term planting easily causes the degeneration of kind, and this modes of reproduction can not improve kind and upgrade.The problems such as in addition, its disease and pest is serious, and cultivation period is long become increasingly conspicuous, and have had a strong impact on the seed output and quality of water chestnut.Further, Water Chestnuts carries multiple virus disease, and cause water chestnut quality to decline, production rate decline, this exists grave danger to the sound development of water chestnut industry.How to solve the kind sexual involution of bulb, improve water chestnut quality, improve the breeding coefficient of water chestnut, become current water chestnut and produced upper problem in the urgent need to address.
Therefore, adopt seed to study as propagulum, be a brand-new field for water chestnut, breaking through this field will have an immense impact on to water chestnut breeding and industry development.At present, the research that industry is carried out it is considerably less, and this is also determined by water chestnut seed self character, and its form is very tiny, length and width are all less than 1mm, thickness is less than 0.5mm, grows, and wrapped up by outer kind of shell of one deck compact structure in reversed cardioid, be difficult to germinate without process seed, even if the seed after germinateing, be subject to the little impact of endosperm and cause nutrition supply not enough, surviving rate is also very low.Therefore, in the plantation of traditional water chestnut, water chestnut seed is always out in the cold as propagulum, and its excellent heritability can not get embodying.Moreover the substratum of prior art to applicable water chestnut seed germination not yet has research.
Summary of the invention
The object of the present invention is to provide a kind of method and the used medium that promote water chestnut seed germination.The method and substratum used can improve percentage of germination and the surviving rate of water chestnut seed.
For solving above technical problem, the invention provides following technical scheme:
At the substratum promoting to use in the method for water chestnut seed germination, described substratum is made up of MS substratum, 6-BA (6-benzyl aminoadenine), NAA (naphthylacetic acid), sucrose.
Preferably: described often liter of MS substratum adds 0.5-1.0mg6-BA, 0.05-0.1mgNAA, 25g sucrose, and described medium pH is 5.8-6.4.
The method of the promotion water chestnut seed germination utilizing above-mentioned substratum to carry out, comprises the following steps:
1) seed soaking after water chestnut seed shell being peeled off;
2) by step 1) gained seed carries out disinfection and cleans;
3) seed culture: by step 2) gained seed moves to substratum, and under 26-30 DEG C of temperature, 8000-10000Lx illumination condition, illumination every day 8-12h cultivates, and culture cycle is 15-25d.
Preferably: step 1) described seed soaking is until water temperature naturally cools to room temperature in warm water seed being soaked in 50-60 DEG C.
Preferably: step 2) described sterilization comprises alcohol disinfecting and mercuric chloride sterilization, described alcohol disinfecting is by step 1) gained seed be placed in volume fraction be 75% spirituous solution to sterilize 5s, described mercuric chloride sterilization be seed after alcohol disinfecting is placed in volume fraction be 0.1% mercuric chloride solution to sterilize 7-10min.
Preferably: step 2) described cleaning utilizes sterilized water to carry out 6-8 cleaning.
The present invention can reduce kind of shell on the one hand to the inhibition germinateed, make water chestnut seed absorb moisture fast on the other hand, reach the requirement of germination after being peeled off by water chestnut kind shell and removing.Seed, after warm water soaking, can significantly reduce seed germination inhibition, be conducive to seed germination.In addition, hormone in medium and nutrition supply can promote seed germination and improve the seed viability after germinateing.The inventive method operating procedure is simple, equipment investment is few, cost is low, is easy to expand the scale of production.Rate of emergence of the present invention reaches more than the percentage of germination of 60%, and the seed viability after germination reaches 100%.
Embodiment
Below in conjunction with specific embodiment, elaboration detailed is further done to the present invention, but embodiments of the present invention are not limited to the scope that embodiment represents.These embodiments only for illustration of the present invention, but not for limiting the scope of the invention.In addition, after reading content of the present invention, those skilled in the art can do various amendment to the present invention, and these equivalent variations fall within appended claims limited range of the present invention equally.
Embodiment 1
Fixed by water chestnut seed tweezers, the kind shell will with scalper with dense structure adopts mechanical effect to peel off, by putting into the warm water of 50 DEG C without breakage, full grains, 100 seeds that glossiness is good, until water temperature naturally cools to room temperature.Seed is moved to the spirituous solution sterilization 5s that volume fraction is 75%, then move to the mercuric chloride solution immersion 7min that volume fraction is 0.1%, move to substratum 6 times by sterile water wash, under 26 DEG C of temperature, 8000Lx illumination condition, illumination every day 8h cultivates, and culture cycle is 25d, makes it to germinate.Described substratum is made up of MS substratum, 6-BA, NAA, sucrose, and often liter of MS substratum adds 0.5mg6-BA, 0.05mgNAA, 25g sucrose, pH regulator to 5.8.
Embodiment 2
Fixed by water chestnut seed tweezers, the kind shell will with scalper with dense structure adopts mechanical effect to peel off, by putting into the warm water of 55 DEG C without breakage, full grains, 100 seeds that glossiness is good, until water temperature naturally cools to room temperature.Seed is moved to the spirituous solution sterilization 5s that volume fraction is 75%, move to the mercuric chloride solution immersion 8min that volume fraction is 0.1% again, substratum is moved to 7 times by sterile water wash, under 28 DEG C of temperature, 9000Lx illumination condition, illumination every day 10h cultivates, culture cycle is 20d, makes it to germinate.Described substratum is made up of MS substratum, 6-BA, NAA, and often liter of MS substratum adds 0.8mg6-BA, 0.08mgNAA, 25g sucrose, pH regulator to 6.0.
Embodiment 3
Fixed by water chestnut seed tweezers, the kind shell will with scalper with dense structure adopts mechanical effect to peel off, by putting into the warm water of 60 DEG C without breakage, full grains, 100 seeds that glossiness is good, until water temperature naturally cools to room temperature.Seed is moved to the spirituous solution sterilization 5s that volume fraction is 75%, move to the mercuric chloride solution immersion 10min that volume fraction is 0.1% again, substratum is moved to 8 times by sterile water wash, under 30 DEG C of temperature, 10000Lx illumination condition, illumination every day 12h cultivates, culture cycle is 15d, makes it to germinate.Described substratum is made up of MS substratum, 6-BA, NAA, and often liter of MS substratum adds 1.0mg6-BA, 0.1mgNAA, 25g sucrose, pH regulator to 6.4.
In order to the effect of the present invention in the method and substratum that promote water chestnut seed germination is better described, makes following control group and test:
Experimental group 1
The water chestnut seed 100 not being abolished shell puts into the warm water of 60 DEG C, until water temperature naturally cools to room temperature.Seed is moved to the spirituous solution sterilization 5s that volume fraction is 75%, then move to volume fraction be 0.1% mercuric chloride solution soak 7min, move to yarn bed with after sterile water wash 6 times, under 28 DEG C of temperature, 8000Lx illumination condition, illumination every day 8h cultivates, and culture cycle is 20d, makes it to germinate.
Experimental group 2
Shell is abolished, selects the warm water putting into 60 DEG C without breakage, full grains, 100, water chestnut seed that glossiness is good, until water temperature naturally cools to room temperature.Seed is moved to the spirituous solution sterilization 5s that volume fraction is 75%, then move to volume fraction be 0.1% mercuric chloride solution soak 7min, move to yarn bed with after sterile water wash 6 times, under 28 DEG C of temperature, 8000Lx illumination condition, illumination every day 8h cultivates, and culture cycle is 20d, makes it to germinate.
Experimental group 3
Fixed by water chestnut seed tweezers, the kind shell will with scalper with dense structure adopts mechanical effect to peel off, and puts into normal temperature sterilized water soak about 3h by without breakage, full grains, 100 seeds that glossiness is good.Seed is moved to the spirituous solution sterilization 5s that volume fraction is 75%, then move to the mercuric chloride solution immersion 7min that volume fraction is 0.1%, move to substratum 6 times by sterile water wash, under 28 DEG C of temperature, 8000Lx illumination condition, illumination every day 8h cultivates, and culture cycle is 20d, makes it to germinate.Described substratum is made up of MS substratum, 6-BA, NAA, and often liter of MS substratum adds 1.0mg6-BA, 0.1mgNAA, 25g sucrose, pH regulator to 5.8.
Experimental group 4
Fixed by water chestnut seed tweezers, the kind shell will with scalper with dense structure adopts mechanical effect to peel off, by putting into the warm water of 60 DEG C without breakage, full grains, 100 seeds that glossiness is good, until water temperature naturally cools to room temperature.Seed is moved to the spirituous solution sterilization 5s that volume fraction is 75%, then move to the mercuric chloride solution immersion 7min that volume fraction is 0.1%, move to substratum 6 times by sterile water wash, under 28 DEG C of temperature, 8000Lx illumination condition, illumination every day 8h cultivates, and culture cycle is 20d, makes it to germinate.Described substratum is made up of MS substratum, 6-BA, NAA, and often liter of MS substratum adds 1.0mg6-BA, 0.1mgNAA, 25g sucrose, pH regulator to 5.8.
Tradition water chestnut is all adopt propagation by corm, the research of water chestnut seed germination is a frontier, so four experimental group contrasts of the present invention's design all adopt water chestnut seed to carry out, and by the key element in the present invention, comparative study is carried out to rate of emergence and the surviving rate after germinateing, the results are shown in Table 1.
Table 1 is not abolished shell seed group and is abolished shell seed group percentage of germination and seed viability compares
As can be seen from experimental data, placed on yarn bed by the seed not abolishing shell in first group of experiment and cultivate, its percentage of germination is 0%; Under the seed abolishing shell being placed on the cultivation of yarn bed in second group of experiment, percentage of germination can be brought up to 30%, but due to nutrition supply not enough, seed can not send out roots in time must, also cannot transplant, cause surviving rate to be 0%; Placed in substratum by the seed abolishing shell in 3rd group of experiment and cultivate, its percentage of germination can reach 40%, and surviving rate is 100%; After the seed abolishing shell first being used 60 DEG C of warm water process in 4th group of experiment, then place in substratum and cultivate, rate of emergence reaches 60%, and surviving rate also can reach 100%.Comprehensively above-mentioned, use advantage of the present invention to be: the percentage of germination improving water chestnut seed, and ensure the surviving rate after germinateing.
Claims (6)
1., at the substratum promoting to use in the method for water chestnut seed germination, it is characterized in that: described substratum is made up of MS substratum, 6-BA, NAA, sucrose.
2. substratum according to claim 1, is characterized in that: described often liter of MS substratum adds 0.5-1.0mg6-BA, 0.05-0.1mgNAA, 25g sucrose, and described medium pH is 5.8-6.4.
3. the method for the promotion water chestnut seed germination utilizing substratum described in claim 1 or 2 to carry out, comprises the following steps:
1) seed soaking after water chestnut seed shell being peeled off;
2) by step 1) gained seed carries out disinfection and cleans;
3) seed culture: by step 2) gained seed moves to substratum, and under 26-30 DEG C of temperature, 8000-10000Lx illumination condition, illumination every day 8-12h cultivates, and culture cycle is 15-25d.
4. promoting the method for water chestnut seed germination according to claim 3, it is characterized in that: step 1) described seed soaking is until water temperature naturally cools to room temperature in warm water seed being soaked in 50-60 DEG C.
5. promote the method for water chestnut seed germination according to claim 3, it is characterized in that: step 2) described sterilization comprises alcohol disinfecting and mercuric chloride is sterilized, described alcohol disinfecting is by step 1) gained seed be placed in volume fraction be 75% spirituous solution to sterilize 5s, described mercuric chloride sterilization be by alcohol disinfecting after seed be placed in volume fraction be 0.1% mercuric chloride solution to sterilize 7-10min.
6. promoting the method for water chestnut seed germination according to claim 3, it is characterized in that: step 2) described cleaning utilizes sterilized water to carry out 6-8 time to clean.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410457519.2A CN104261972A (en) | 2014-09-10 | 2014-09-10 | Method for promoting water chestnut seeds to sprout and used culture medium |
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| CN201410457519.2A CN104261972A (en) | 2014-09-10 | 2014-09-10 | Method for promoting water chestnut seeds to sprout and used culture medium |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106717280A (en) * | 2016-12-29 | 2017-05-31 | 吴萍 | A kind of processing method of tea tree seed |
| CN107711381A (en) * | 2017-11-21 | 2018-02-23 | 五河县金塘水产养殖有限公司 | A kind of fast seedling-cultivating method of water chestnut |
| CN107710957A (en) * | 2017-11-03 | 2018-02-23 | 五河县金塘水产养殖有限公司 | A kind of processing method for promoting water chestnut germination |
| CN107864713A (en) * | 2017-11-18 | 2018-04-03 | 蚌埠市徽吉星农业科技农民专业合作社 | A kind of processing method for accelerating China tree germination |
| CN109804749A (en) * | 2019-03-25 | 2019-05-28 | 西安同人五凤农业有限公司 | A kind of method that elaeagnus mollis seed stratification is taken root |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487829A (en) * | 2011-12-19 | 2012-06-13 | 广州甘蔗糖业研究所湛江甘蔗研究中心 | Method of comprehensive detoxification and rapid propagation for starch-type water chestnut |
| CN103688862A (en) * | 2013-12-16 | 2014-04-02 | 广西壮族自治区农业科学院生物技术研究所 | Method for efficiently propagating water chestnut tissue culture seedlings |
-
2014
- 2014-09-10 CN CN201410457519.2A patent/CN104261972A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487829A (en) * | 2011-12-19 | 2012-06-13 | 广州甘蔗糖业研究所湛江甘蔗研究中心 | Method of comprehensive detoxification and rapid propagation for starch-type water chestnut |
| CN103688862A (en) * | 2013-12-16 | 2014-04-02 | 广西壮族自治区农业科学院生物技术研究所 | Method for efficiently propagating water chestnut tissue culture seedlings |
Non-Patent Citations (1)
| Title |
|---|
| 李双梅等: "不同处理方法对荸荠种子发芽的影响", 《中国蔬菜》, no. 8, 30 April 2011 (2011-04-30), pages 55 - 59 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106717280A (en) * | 2016-12-29 | 2017-05-31 | 吴萍 | A kind of processing method of tea tree seed |
| CN107710957A (en) * | 2017-11-03 | 2018-02-23 | 五河县金塘水产养殖有限公司 | A kind of processing method for promoting water chestnut germination |
| CN107864713A (en) * | 2017-11-18 | 2018-04-03 | 蚌埠市徽吉星农业科技农民专业合作社 | A kind of processing method for accelerating China tree germination |
| CN107711381A (en) * | 2017-11-21 | 2018-02-23 | 五河县金塘水产养殖有限公司 | A kind of fast seedling-cultivating method of water chestnut |
| CN109804749A (en) * | 2019-03-25 | 2019-05-28 | 西安同人五凤农业有限公司 | A kind of method that elaeagnus mollis seed stratification is taken root |
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