CN102907324B - Method for rapidly and efficiently rooting pistacia chinensis bunge by taking stem tips and stem segments as explants - Google Patents
Method for rapidly and efficiently rooting pistacia chinensis bunge by taking stem tips and stem segments as explants Download PDFInfo
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- CN102907324B CN102907324B CN201210437288.XA CN201210437288A CN102907324B CN 102907324 B CN102907324 B CN 102907324B CN 201210437288 A CN201210437288 A CN 201210437288A CN 102907324 B CN102907324 B CN 102907324B
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Abstract
The invention belongs to the technical field of plant biology, particularly relating to a method for rapidly and efficiently rooting pistacia chinensis bunge by taking stem tips and stem segments as explants. In the method, inoculated culture comprises the following specific operation steps of inoculating disinfected and rinsed seeds on an MS culture medium without any hormone, wherein the culture conditions are as follow: 20 to 30 DEG C of temperature, 1,500 to 2,500 lx of illumination intensity and 14 h/d of illumination time; explant culture comprises the following specific operation steps of taking pistacia chinensis bunge seed seedlings; and inoculating the stem tips and the stem segments which are cut off on an ultra-clean workbench onto a rooting culture medium with 1/2 WPM inorganic salt, MS organic matter, 1.0 mg/L of IBA (Indole Butyric Acid), 0.2 mg/L of NAA (Naphthyl Acetic Acid), 30 g/L of sucrose and 6.8 g/L of agar powder, wherein the pH of the rooting culture medium is 5.8. According to the method, the rooting time of the pistacia chinensis bunge is further shortened, and a fast, high-efficiency, high-quality, wide-application range method for rooting the pistacia chinensis bunge is obtained.
Description
Technical field
The invention belongs to plant biotechnology field, be specifically related to a kind of rapidly and efficiently rooting method of Chinese pistache that stem apex and stem section be explant of take.
Background technology
Chinese pistache (
pistacia chinensisbunge) dioecism, has another name called pattern wood, pattern tree etc., belongs to Anacardiaceae pistache deciduous tree.It is drought-resistant, saline and alkaline, barren, and strong adaptability has a very wide distribution, be a kind of good greening, with material, view and admire, medicinal and oil plant seeds.Bright leaf can extract aromatic oil, has the drug effects such as clearing heat and detoxicating.Root, stem, leaf, bark, Ye Junke are used as medicine.Blade, seed and bark etc. are rich in tannin, are the first-class raw materials of producing tannin extract.Male and female plant has different economic worths, the tough and tensile densification of staminiferous plant timber, have a fine grain, corrosion resistance is strong, can build, furniture and engraving material.Female plant fruit oil content 35%; seed oil content 40%, plants benevolence oil content 56%, is the excellent raw material of preparing biological diesel oil; by expert, classified as following one of the biodiesel raw material seeds of development potentiality that have most for 20 years, to solving energy crisis current and future, there is important effect.
At present, Chinese pistache, mainly by sowing, cuttage and propagation by grafiting, can not meet the great demand to Chinese pistache seedling both at home and abroad far away.The application of Chinese pistache group culturation rapid propagating technology can be for providing the seedling of a large amount of high-qualitys on producing, wherein Chinese pistache group training seedling rooting is that Chinese pistache is grown into the requisite step of seedling, and the Chinese pistache group training speed of seedling rooting and the height of rooting rate indicate the success or failure of experiment.Academic circles at present is increasing to the research of Chinese pistache tissue-culturing rapid propagation, such as Cheng Shiping, Shi Jiang etc. adopt 1/2 WPM+1.0 mg/L IBA+0.2 mg/L NAA as root media, cultivates 35 days, and rooting rate reaches 100%.What respects the employing 1/2 DKW+6.0 mg/L IBA such as room as root media, cultivates 30 days, and rooting rate reaches 91.15%.The people such as Guo Yuxia adopt MS+10.0 mg/L IBA+3.0 mg/L IAA+30 g/L sucrose+4 g/L agar as root media, cultivate 30 days, and rooting rate reaches more than 60%.Yet, its quality of rooting, to the adaptability of material, shorten rootage duration aspect and have much room for improvement.
Summary of the invention
The object of the invention is to find a kind of simple, convenient and method that can make Chinese pistache rapidly and efficiently take root.
The technical solution adopted in the present invention is, a kind ofly take rapidly and efficiently rooting method of Chinese pistache that stem apex and stem section be explant, comprises the following steps:
(1) selection: choose the Chinese pistache seed of the mature and plump in the first tenday period of a month in the last ten-days period in September to October then;
(2) disinfect: the duricrust that first removes Chinese pistache seed outside, then seed is placed in 75% ethanolic solution and soaks 30 seconds, with sterile water washing 3-5 time, then seed is placed in 0.1% mercuric chloride solution and soaks 8-10 minute, ceaselessly shake during this time, with sterile water washing 3-5 time;
(3) inoculated and cultured: the seed after sterilization rinsing is seeded on the MS medium that does not add any hormone, and condition of culture is temperature 20-30 ℃, intensity of illumination 1500-2500 lx, light application time 14 h/d, obtain the aseptic seedling of Chinese pistache;
(4) explant is cultivated: get the growth aseptic seedling of growing directly from seeds of Chinese pistache of 30 days, on superclean bench, cut stem apex and stem section, by stem apex and stem section be seeded in be added with mineral salt in WPM 1/2, on the pH of organic matter, 1.0 mg/L IBA, 0.2 mg/L NAA, 30 g/L sucrose and 6.8 g/L agar powders in the MS root media that is 5.8, explant has little Bai root to grow for 6 days, cultivate after 25 days, taken root, obtained complete Chinese pistache aseptic seedlings.
the invention has the beneficial effects as follows:in first culture process, the WPM of take is fine as basic medium effect, and MS medium effect is far short of what is expected; In seed germination and growth of seedling process, MS medium is more better than WPM medium.The present invention by choosing active ingredient from WPM and MS medium, optimize proportioning, mix with other medium component again, combine the advantage of these two kinds of medium of WPM and MS, the rootage duration of Chinese pistache is further shortened, obtained a kind of Chinese pistache rooting method quick, efficient, high-quality, applied widely.
Accompanying drawing explanation
Fig. 1 is for adopting 30 days situations of taking root of Chinese pistache group training seedling of the present invention.
Embodiment
Below embodiments of the invention are elaborated: the present embodiment is implemented take technical solution of the present invention under prerequisite, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
The Chinese pistache that stem apex and stem section are explant is a rooting method rapidly and efficiently, comprises the following steps:
(1) selection: the Chinese pistache seed of choosing mature and plump;
(2) disinfect: the duricrust that first removes Chinese pistache seed outside, then seed is placed in 75% ethanolic solution and soaks 30 seconds, with sterile water washing 4 times, then seed is placed in 0.1% mercuric chloride solution and soaks 9 minutes, ceaselessly shake during this time, with sterile water washing 4 times;
(3) inoculated and cultured: the seed after sterilization rinsing is seeded on the MS medium that does not add any hormone, and condition of culture is 25 ℃ of temperature, intensity of illumination 2000 lx, light application time 14 h/d, obtain the aseptic seedling of Chinese pistache;
(4) explant is cultivated: get the growth aseptic seedling of growing directly from seeds of Chinese pistache of 30 days, on aseptic platform, cut stem apex and stem section, on the root media that stem apex and stem section are seeded in and are added with that 1/2 WPM is inorganic, MS is organic, the pH of 1.0 mg/L IBA, 0.2 mg/L NAA, 30 g/L sucrose and 6.8 g/L agar powders is 5.8, explant has little Bai root to grow for 6 days, cultivate after 25 days, taken root, obtain complete Chinese pistache aseptic seedlings, within 25 days, statistics rooting rate reaches 100%, has reached the object of rapidly and efficiently taking root.
Fig. 1 is for adopting 30 days situations of taking root of Chinese pistache group training seedling of the present invention.
Above-described embodiment result shows: the present invention is rapidly and efficiently rooting method of a kind of effective Chinese pistache, and the Chinese pistache that adopts the present invention to cultivate has the advantage fast, rooting rate is high of taking root.
Claims (1)
1. take a rapidly and efficiently rooting method of Chinese pistache that stem apex and stem section be explant, it is characterized in that, comprise the following steps:
(1) selection: choose the Chinese pistache seed of the mature and plump in the first tenday period of a month in the last ten-days period in September to October then;
(2) disinfect: the duricrust that first removes Chinese pistache seed outside, then seed is placed in 75% ethanolic solution and soaks 30 seconds, with sterile water washing 3-5 time, then seed is placed in 0.1% mercuric chloride solution and soaks 8-10 minute, ceaselessly shake during this time, with sterile water washing 3-5 time;
(3) inoculated and cultured: the seed after sterilization rinsing is seeded on the MS medium that does not add any hormone, and condition of culture is temperature 20-30 ℃, intensity of illumination 1500-2500 lx, light application time 14 h/d, obtain the aseptic seedling of Chinese pistache;
(4) explant is cultivated: get the growth aseptic seedling of growing directly from seeds of Chinese pistache of 30 days, on superclean bench, cut stem apex and stem section, by stem apex and stem section be seeded in be added with mineral salt in WPM 1/2, on the pH of organic matter, 1.0 mg/L IBA, 0.2 mg/L NAA, 30 g/L sucrose and 6.8 g/L agar powders in the MS root media that is 5.8, explant has little Bai root to grow for 6 days, cultivate after 25 days, taken root, obtained complete Chinese pistache aseptic seedlings.
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| CN201210437288.XA CN102907324B (en) | 2012-11-06 | 2012-11-06 | Method for rapidly and efficiently rooting pistacia chinensis bunge by taking stem tips and stem segments as explants |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010046434A1 (en) * | 2008-10-22 | 2010-04-29 | Basf Se | Use of bleacher herbicides on cultivated plants |
| CN102138524A (en) * | 2011-01-07 | 2011-08-03 | 中国科学院合肥物质科学研究院 | Method for quickly propagating pistacia chinensis bunge by using stem sections |
| CN102301959A (en) * | 2011-08-17 | 2012-01-04 | 中国科学院合肥物质科学研究院 | Method for regenerating Chinese pistache plant by taking cotyledons as explants |
| CN102498793A (en) * | 2011-10-22 | 2012-06-20 | 中国科学院合肥物质科学研究院 | Method for removing endophytes from pistacia chinensis bunge seeds and quickly germinating pistacia chinensis bunge seeds |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10130254A (en) * | 1996-11-01 | 1998-05-19 | Jumoku Seiri Kinousei Butsushitsu Gijutsu Kenkyu Kumiai | Dihydrocoumarin-based compound and estrogenic agent |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010046434A1 (en) * | 2008-10-22 | 2010-04-29 | Basf Se | Use of bleacher herbicides on cultivated plants |
| CN102138524A (en) * | 2011-01-07 | 2011-08-03 | 中国科学院合肥物质科学研究院 | Method for quickly propagating pistacia chinensis bunge by using stem sections |
| CN102301959A (en) * | 2011-08-17 | 2012-01-04 | 中国科学院合肥物质科学研究院 | Method for regenerating Chinese pistache plant by taking cotyledons as explants |
| CN102498793A (en) * | 2011-10-22 | 2012-06-20 | 中国科学院合肥物质科学研究院 | Method for removing endophytes from pistacia chinensis bunge seeds and quickly germinating pistacia chinensis bunge seeds |
Non-Patent Citations (3)
| Title |
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| JP特开平10130254A 1998.05.19 |
| 程世平等.黄连木茎尖组织快繁技术研究.《江苏农业科学》.2010,(第6期),第66页1.2.1、1.2.3、第67页右栏第1段及表4. |
| 黄连木茎尖组织快繁技术研究;程世平等;《江苏农业科学》;20101231(第6期);第66页1.2.1、1.2.3、第67页右栏第1段及表4 * |
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