CN102498397B - 核酸分析 - Google Patents

核酸分析 Download PDF

Info

Publication number
CN102498397B
CN102498397B CN201080041496.7A CN201080041496A CN102498397B CN 102498397 B CN102498397 B CN 102498397B CN 201080041496 A CN201080041496 A CN 201080041496A CN 102498397 B CN102498397 B CN 102498397B
Authority
CN
China
Prior art keywords
microvesicles
nucleic acid
rna
urine
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201080041496.7A
Other languages
English (en)
Chinese (zh)
Other versions
CN102498397A (zh
Inventor
雷莱塔·M·鲁索
凯文·C·米兰达
约翰·斯科格
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Hospital Corp
Original Assignee
General Hospital Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Hospital Corp filed Critical General Hospital Corp
Publication of CN102498397A publication Critical patent/CN102498397A/zh
Application granted granted Critical
Publication of CN102498397B publication Critical patent/CN102498397B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D249/00—Heterocyclic compounds containing five-membered rings having three nitrogen atoms as the only ring hetero atoms
    • C07D249/02—Heterocyclic compounds containing five-membered rings having three nitrogen atoms as the only ring hetero atoms not condensed with other rings
    • C07D249/08—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/12—Antidiuretics, e.g. drugs for diabetes insipidus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Diabetes (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
CN201080041496.7A 2009-07-16 2010-07-16 核酸分析 Active CN102498397B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US22602509P 2009-07-16 2009-07-16
US22610609P 2009-07-16 2009-07-16
US61/226,025 2009-07-16
US61/226,106 2009-07-16
PCT/US2010/042365 WO2011009104A1 (en) 2009-07-16 2010-07-16 Nucleic acid analysis

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201610128465.4A Division CN105779436A (zh) 2009-07-16 2010-07-16 核酸分析

Publications (2)

Publication Number Publication Date
CN102498397A CN102498397A (zh) 2012-06-13
CN102498397B true CN102498397B (zh) 2016-04-06

Family

ID=43449850

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201610128465.4A Pending CN105779436A (zh) 2009-07-16 2010-07-16 核酸分析
CN201080041496.7A Active CN102498397B (zh) 2009-07-16 2010-07-16 核酸分析

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CN201610128465.4A Pending CN105779436A (zh) 2009-07-16 2010-07-16 核酸分析

Country Status (13)

Country Link
US (3) US20110195426A1 (enExample)
EP (1) EP2454588B1 (enExample)
JP (3) JP5909444B2 (enExample)
KR (2) KR20180029105A (enExample)
CN (2) CN105779436A (enExample)
AU (1) AU2010273928A1 (enExample)
BR (1) BR112012001077A2 (enExample)
CA (1) CA2768371A1 (enExample)
DK (1) DK2454588T3 (enExample)
ES (1) ES2547715T3 (enExample)
IN (1) IN2012DN00866A (enExample)
SG (2) SG177677A1 (enExample)
WO (1) WO2011009104A1 (enExample)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007103572A2 (en) * 2006-03-09 2007-09-13 Aethlon Medical, Inc. Extracorporeal removal of microvesicular particles
US11266736B2 (en) 2008-04-17 2022-03-08 Vin De Bona Trading Company Pte Ltd Method of painting micro vesicles
WO2011031892A1 (en) 2009-09-09 2011-03-17 The General Hospital Corporation Use of microvesicles in analyzing kras mutations
WO2011031877A1 (en) * 2009-09-09 2011-03-17 The General Hospital Corporation Use of microvesicles in analyzing nucleic acid profiles
WO2011156763A1 (en) * 2010-06-11 2011-12-15 Hitachi Chemical Co., Ltd. Methods for characterizing kidney function
WO2012031008A2 (en) 2010-08-31 2012-03-08 The General Hospital Corporation Cancer-related biological materials in microvesicles
EP2638057B1 (en) * 2010-11-10 2019-03-06 Exosome Diagnostics, Inc. Method for isolation of nucleic acid containing particles and extraction of nucleic acids therefrom
SG192226A1 (en) * 2011-01-31 2013-09-30 Esoterix Genetic Lab Llc Methods for enriching microparticles or nucleic acids using binding molecules
EP2707381B1 (en) * 2011-05-11 2019-08-14 Exosome Diagnostics, Inc. Nucleic acid extraction from heterogeneous biological materials
US9719129B2 (en) 2011-06-10 2017-08-01 Hitachi Chemical Co., Ltd. Methods for isolating vesicles from biological fluids
EP3492606B1 (en) 2011-08-22 2023-10-04 Exosome Diagnostics, Inc. Urine biomarkers
WO2013071239A1 (en) * 2011-11-10 2013-05-16 Exosome Diagnostics, Inc. Cerebrospinal fluid assay
WO2014036391A1 (en) 2012-08-30 2014-03-06 Exosome Diagnostics, Inc. Controls for nucleic acid assays
US20150252428A1 (en) * 2012-10-03 2015-09-10 Exosome Diagnostics, Inc. Use of microvesicles in diagnosis, prognosis, and treatment of medical diseases and conditions
EP2904118B1 (en) * 2012-10-05 2019-03-20 Hitachi Chemical Co., Ltd. Urine exosome mrnas and methods of using same to detect diabetic nephropathy
EP2906717B8 (en) 2012-10-11 2019-03-27 Vin-de-Bona Trading Company Pte Ltd Method of painting microvesicles
HUE049003T2 (hu) 2013-03-13 2020-09-28 Univ Miami Eljárás mikrovezikulumok izolálására és tisztítására sejttenyészet-felülúszóból és biológiai folyadékból
US9662649B2 (en) 2013-05-06 2017-05-30 Hitachi Chemical Company America, Ltd. Devices and methods for capturing target molecules
JP2016526922A (ja) 2013-08-06 2016-09-08 エクソサム ダイアグノスティクス，インコーポレイティド 尿バイオマーカーコホート、遺伝子発現特性、およびその使用の方法
AU2014346788B8 (en) 2013-11-06 2021-01-28 Arizona Board Of Regents On Behalf Of The Unviersity Of Arizona Method for subtyping lymphoma types by means of expression profiling
US10494676B2 (en) * 2013-12-02 2019-12-03 Albert-Ludwigs-Universitaet Freiburg Process for the diagnosis of cancer by using exosomes
WO2015153732A2 (en) 2014-04-01 2015-10-08 Cornell University Use of double-stranded dna in exosomes: a novel biomarker in cancer detection
CN106232815A (zh) * 2014-04-22 2016-12-14 特罗瓦基因公司 浓缩尿中的核酸
CN104031915A (zh) * 2014-06-17 2014-09-10 复旦大学附属肿瘤医院 用于结直肠癌预后的基因片段及其应用
US10266895B2 (en) 2014-11-05 2019-04-23 Hitachi Chemical Company Ltd. Exosomes and microvesicles in intestinal luminal fluids and stool and use of same for the assessment of inflammatory bowel disease
WO2016077537A1 (en) 2014-11-12 2016-05-19 Hitachi Chemical Co., Ltd. Method and device for diagnosing organ injury
WO2016172598A1 (en) * 2015-04-22 2016-10-27 The Broad Institute Inc. Exosomes and uses thereof
GB2539497A (en) 2015-06-19 2016-12-21 Airbus Operations Ltd A Rotational Joint For An Aircraft Folding Wing
JP6624704B2 (ja) 2015-08-31 2019-12-25 日立化成株式会社 尿路上皮疾患の評価のための分子法
US11702702B2 (en) 2016-04-15 2023-07-18 Predicine, Inc. Systems and methods for detecting genetic alterations
WO2018057820A1 (en) 2016-09-21 2018-03-29 Predicine, Inc. Systems and methods for combined detection of genetic alterations
EP3455355B1 (en) * 2016-05-13 2022-04-13 Exosome Diagnostics, Inc. Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free dna from biofluids
EP3635139A1 (en) 2017-05-17 2020-04-15 Exosome Diagnostics, Inc. Microvesicle nucleic acids and/or proteins and uses thereof as markers of renal transplant rejection
EP3652315A4 (en) 2017-07-12 2021-09-01 Exosome Diagnostics, Inc. METHODS FOR ISOLATION AND ENRICHMENT OF EXTRACELLULAR VESICLE POPULATIONS DERIVED FROM BIOFLUIDS, AND ASSOCIATED METHODS OF USE
JP7069587B2 (ja) * 2017-07-31 2022-05-18 東ソー株式会社 分析機器の洗浄方法
WO2019116234A1 (en) * 2017-12-12 2019-06-20 Nestec S.A. Methods for determining microbiome ribosomal rna composition changes
JP7444386B2 (ja) * 2018-06-07 2024-03-06 株式会社Ｌｓｉメディエンス ヒト尿からのマイクロベシクルの分離方法及び分析方法
US12258587B2 (en) 2018-11-20 2025-03-25 Exosome Diagnostics, Inc. Compositions and methods for internal controls of microvesicle isolations
CN109856259A (zh) * 2018-12-29 2019-06-07 南通大学附属医院 一种血清胰腺癌外泌体特异蛋白及其再验证过程
CN114958834A (zh) * 2020-03-09 2022-08-30 中山大学 一种adar蛋白细胞内高效结合底物及应用
WO2021243206A1 (en) 2020-05-29 2021-12-02 Exosome Diagnostics, Inc. Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection
CN116057185A (zh) 2020-08-15 2023-05-02 雷杰纳荣制药公司 治疗具有编码降钙素受体(calcr)的变体核酸分子的受试者的肥胖症
EP4370712A1 (en) 2021-07-16 2024-05-22 Exosome Diagnostics, Inc. Methods of detecting sjögren's syndrome using salivary exosomes
EP4479561A1 (en) 2022-02-18 2024-12-25 Exosome Diagnostics, Inc. Use of microvesicle signatures in the identification and treatment of renal disorders
CN114480656B (zh) * 2022-03-07 2023-12-26 温州医科大学附属眼视光医院 Slc12a3和/或slc12a9在作为葡萄膜黑色素瘤治疗和预后检测指标中的应用
EP4584397A1 (en) 2022-09-07 2025-07-16 Exosome Diagnostics, Inc. Methods of detecting sjögren's syndrome using salivary exosomes

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002099064A2 (en) * 2001-06-05 2002-12-12 Gene Logic, Inc. Rna purification methods
US20060223072A1 (en) * 2005-03-31 2006-10-05 Boyes Barry E Methods of using a DNase I-like enzyme
US20080287669A1 (en) * 2007-05-16 2008-11-20 Braman Jeffrey C Methods and compositions for identifying compounds useful in nucleic acid purification
WO2009036236A1 (en) * 2007-09-14 2009-03-19 The Ohio State University Research Foundation Mirna expression in human peripheral blood microvesicles and uses thereof
CN102084000A (zh) * 2008-02-01 2011-06-01 总医院有限公司 微泡在医学疾病和病况的诊断、预后以及治疗中的用途

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639611A (en) 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
US5219727A (en) 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
US5840867A (en) 1991-02-21 1998-11-24 Gilead Sciences, Inc. Aptamer analogs specific for biomolecules
CA2218875C (en) 1991-07-23 2000-11-07 The Research Foundation Of State University Of New York Improvements in the in situ pcr
US5582981A (en) 1991-08-14 1996-12-10 Gilead Sciences, Inc. Method for identifying an oligonucleotide aptamer specific for a target
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US5639606A (en) 1993-04-06 1997-06-17 The University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
US5556773A (en) 1993-08-06 1996-09-17 Yourno; Joseph Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes
ES2347854T5 (es) * 1996-03-26 2014-07-24 Michael S. Kopreski Método que permite el uso de RNA extracelular extraído de plasma o suero para detectar, monitorizar o evaluar un cáncer
AU735416B2 (en) 1996-11-06 2001-07-05 Sequenom, Inc. Dna diagnostics based on mass spectrometry
US6994960B1 (en) 1997-05-28 2006-02-07 The Walter And Eliza Hall Institute Of Medical Research Nucleic acid diagnostics based on mass spectrometry or mass separation and base specific cleavage
US6004755A (en) 1998-04-07 1999-12-21 Incyte Pharmaceuticals, Inc. Quantitative microarray hybridizaton assays
US6204375B1 (en) * 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
FR2788780B1 (fr) 1999-01-27 2001-03-30 Ap Cells Inc Procede de preparation de vesicules membranaires
GB9927320D0 (en) 1999-11-18 2000-01-12 Chiron Spa Exosome separation
US6870021B2 (en) 2000-01-28 2005-03-22 Mip Technologies Ab Functional monomers for molecular recognition and catalysis
US6812023B1 (en) 2000-04-27 2004-11-02 Anosys, Inc. Methods of producing membrane vesicles
US20040241176A1 (en) * 2000-04-27 2004-12-02 Ap Cells. Inc. Method of producing membrane vesicles
US6913879B1 (en) 2000-07-10 2005-07-05 Telechem International Inc. Microarray method of genotyping multiple samples at multiple LOCI
US6525154B1 (en) 2000-07-20 2003-02-25 The Regents Of The University Of California Molecular imprinting for the recognition of peptides in aqueous solution
US6902896B2 (en) * 2001-05-11 2005-06-07 Regents Of The University Of Minnesota Intron associated with myotonic dystrophy type 2 and methods of use
US6696271B2 (en) 2001-08-23 2004-02-24 The Regents Of The University Of California Frozen tissue microarray technology for analysis of RNA, DNA, and proteins
WO2003023065A1 (en) 2001-09-06 2003-03-20 Syngenta Participations Ag Dna methylation patterns
US20030162190A1 (en) 2001-11-15 2003-08-28 Gorenstein David G. Phosphoromonothioate and phosphorodithioate oligonucleotide aptamer chip for functional proteomics
EP2339025B1 (en) 2002-06-26 2013-10-09 Cold Spring Harbor Laboratory Methods for determining the methylation profiles
US7364848B2 (en) 2002-09-02 2008-04-29 Pamgene B.V. Integrated microarray analysis
US7141371B2 (en) 2002-09-06 2006-11-28 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Methods for detecting and localizing DNA mutations by microarray
EP1498144A1 (en) * 2003-07-15 2005-01-19 Universite Pierre Et Marie Curie Paris Vi Extracellular vesicles from non-pathogenic amoebae useful as vehicle for transferring a molecule of interest to an eukaryotic cell
US7384589B2 (en) 2003-08-01 2008-06-10 Lawrence Livermore National Security, Llc Nanoscale molecularly imprinted polymers and method thereof
WO2005121369A2 (en) * 2004-06-02 2005-12-22 Sourcepharm, Inc. Rna-containing microvesicles and methods therefor
US8021847B2 (en) * 2004-06-02 2011-09-20 Proxy Life Science Holdings, Inc. Microvesicle-based compositions and methods
KR20070058441A (ko) * 2004-07-01 2007-06-08 유니버시티 오브 피츠버그 오브 더 커먼웰쓰 시스템 오브 하이어 에듀케이션 면역억제성 엑소솜
US20080187923A1 (en) 2005-04-15 2008-08-07 Cedars-Sinai Medical Center Flow-Cytometric Heteroduplex Analysis for Detection of Genetic Alterations
WO2006119439A2 (en) * 2005-05-03 2006-11-09 Althea Technologies, Inc. Compositions and methods for the analysis of degraded nucleic acids
AU2007221154A1 (en) * 2006-02-24 2007-09-07 Novartis Ag Microparticles containing biodegradable polymer and cationic polysaccharide for use in immunogenic compositions
JP2008035779A (ja) * 2006-08-07 2008-02-21 Mitsubishi Rayon Co Ltd 核酸の分解度を測定する方法と核酸アレイ
EP2613149B1 (en) * 2007-07-25 2014-09-17 University Of Louisville Research Foundation, Inc. Exosome-associated microRNA as a diagnostic marker
US9186405B2 (en) * 2007-08-16 2015-11-17 The Royal Institution For The Advancement Of Learning/Mcgill University Tumor cell-derived microvesicles
EP2198296B1 (en) * 2007-09-05 2015-11-11 Laurentian University Method of using tumour rna integrity to measure response to chemotherapy in cancer patients

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002099064A2 (en) * 2001-06-05 2002-12-12 Gene Logic, Inc. Rna purification methods
US20060223072A1 (en) * 2005-03-31 2006-10-05 Boyes Barry E Methods of using a DNase I-like enzyme
US20080287669A1 (en) * 2007-05-16 2008-11-20 Braman Jeffrey C Methods and compositions for identifying compounds useful in nucleic acid purification
WO2009036236A1 (en) * 2007-09-14 2009-03-19 The Ohio State University Research Foundation Mirna expression in human peripheral blood microvesicles and uses thereof
CN102084000A (zh) * 2008-02-01 2011-06-01 总医院有限公司 微泡在医学疾病和病况的诊断、预后以及治疗中的用途

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Detection of microRNA Expression in Human Peripheral Blood Microvesicles;Melissa Piper Hunter et al;<PLoS ONE>;20081111;第3卷(第11期);1-11页 *
Prostate cancer-derived urine exosomes: a novel approach to biomarkers for prostate cancer;J Nilsson et al;《British Journal of Cancer》;20090428;第100卷;第1603页第2栏最后1段-1604页第1栏第1段 *

Also Published As

Publication number Publication date
US20160348095A1 (en) 2016-12-01
DK2454588T3 (en) 2015-09-28
JP2016116512A (ja) 2016-06-30
BR112012001077A2 (pt) 2020-08-11
SG177677A1 (en) 2012-02-28
WO2011009104A1 (en) 2011-01-20
EP2454588A4 (en) 2012-12-12
KR20120037992A (ko) 2012-04-20
SG2014014617A (en) 2014-05-29
US20170114389A1 (en) 2017-04-27
HK1171083A1 (zh) 2013-03-15
EP2454588A1 (en) 2012-05-23
HK1173213A1 (en) 2013-05-10
CN102498397A (zh) 2012-06-13
KR20180029105A (ko) 2018-03-19
CA2768371A1 (en) 2011-01-20
JP2012533308A (ja) 2012-12-27
US20110195426A1 (en) 2011-08-11
CN105779436A (zh) 2016-07-20
AU2010273928A1 (en) 2012-02-09
EP2454588B1 (en) 2015-07-15
ES2547715T3 (es) 2015-10-08
JP5909444B2 (ja) 2016-04-26
IN2012DN00866A (enExample) 2015-07-10
JP2017131244A (ja) 2017-08-03
AU2010273928A2 (en) 2012-02-09

Similar Documents

Publication Publication Date Title
CN102498397B (zh) 核酸分析
EP3529374B1 (en) Sequencing and analysis of exosome associated nucleic acids
KR101810799B1 (ko) 의학적 질환 및 병태의 진단, 예후, 및 치료에 있어서 미세소포체의 용도
EP3495509B1 (en) Urine biomarker cohorts, gene expression signatures, and methods of use thereof
US20200149036A1 (en) Methods to distinguish rna and dna in a combined preparation
AU2017219022A1 (en) Nucleic acid analysis
HK1171083B (en) Nucleic acid analysis
HK1173213B (en) Nucleic acid analysis
HK40010078A (en) Urine biomarker cohorts, gene expression signatures, and methods of use thereof
HK1158705B (en) Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions
HK1229857B (zh) 尿生物标志物群、基因表达特徵及其使用方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1171083

Country of ref document: HK

C14 Grant of patent or utility model
GR01 Patent grant
REG Reference to a national code

Ref country code: HK

Ref legal event code: GR

Ref document number: 1171083

Country of ref document: HK