CN102487848A - Forced nucleus retention method in nucleated pearl cultivation and nucleus retainer used in method - Google Patents

Forced nucleus retention method in nucleated pearl cultivation and nucleus retainer used in method Download PDF

Info

Publication number
CN102487848A
CN102487848A CN2011103629187A CN201110362918A CN102487848A CN 102487848 A CN102487848 A CN 102487848A CN 2011103629187 A CN2011103629187 A CN 2011103629187A CN 201110362918 A CN201110362918 A CN 201110362918A CN 102487848 A CN102487848 A CN 102487848A
Authority
CN
China
Prior art keywords
pearl
nucleus
steel disc
admittedly
pedestal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN2011103629187A
Other languages
Chinese (zh)
Other versions
CN102487848B (en
Inventor
刘永
张春芳
焦宗垚
于培松
邓陈茂
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangdong Ocean University
Original Assignee
Guangdong Ocean University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangdong Ocean University filed Critical Guangdong Ocean University
Priority to CN2011103629187A priority Critical patent/CN102487848B/en
Priority to PCT/CN2012/076844 priority patent/WO2013071746A1/en
Publication of CN102487848A publication Critical patent/CN102487848A/en
Application granted granted Critical
Publication of CN102487848B publication Critical patent/CN102487848B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/54Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention discloses a forced nucleus retention method in nucleated pearl cultivation and a nucleus retainer used in the method. The nucleus retainer comprises a nucleus retaining bar and a base, wherein the nucleus retaining bar is used for retaining a nucleus, and the base is used for fastening the nucleus retaining bar to a pearl shell. The method includes: fastening the base to the edge of the pearl shell through bolts or fastening the base to the outer side of the pearl shell in an adhered manner, extending one end of the nucleus retaining bar into an outer holding cavity of the pearl shell, exerting certain pressure to hold the nucleus retaining bar onto the outer side of a visceral sac at the position of the nucleus, and fastening the other end of the nucleus retaining bar onto the base; placing the pearl shell in culture water for 10 to 15 days of temporary culture, and removing the nucleus retainer; and forcedly retaining the nucleus which is implanted and has a wound healed in the pearl shell without 'exposure'. The forced nucleus retention method and the nucleus retainer are effective in nucleus fixation, nucleus retention rate of nucleus implanted shells can reach 100% under experimental conditions, and the problem that a nucleus of a large nucleated pearl is 'exposed' during culturing is solved.

Description

Culture the pressure of nucleated pearl and stay kernel method and used solid nuclear device thereof
Technical field
The present invention relates to a kind of method of culturing large-scale nucleated pearl, specifically is that kernel method and the used utensil of this method are stayed in a kind of pressure that is used to culture nucleated pearl.
Background technology
As everyone knows; The nuclear operation method of planting of culturing nucleated pearl is: open an otch with the opening cutter at a certain suitable position of pearl shell internal sac epidermis; Again respectively with nucleus sending device with send the sheet pin that circular beads nuclear and a slice mantle piece are implanted in pearl shell internal sac through this otch, and make mantle piece secrete nacreous one side to be close to pearl and to examine; Mantle piece is organized in vivo and is formed pearl sac around the pearl nuclear breeding, and the nacre of pearl sac emiocytosis is deposited on pearl nuclear surface and is formed with nucleus pearl.Therefore, no matter be breeding seawater nucleated pearl or breed fresh water genuine pearl with pearl, all need be implanted into pearl nuclear at the pearl shell body; But because the shellfish body is to external foreign matter---the rejection effect of pearl nuclear, to plant the nuclear shellfish and all deposit serious " telling " nuclear phenomenon, that is: the pearl nuclear of implanting internal sac is excreted along the passage of implanting pearl nuclear again.This " telling " nuclear phenomenon is particularly serious when using the large pearl shellfish to plant nuclear, and core-remaining rate is only below 10% when for example planting nuclear with wild large nacre.The earliest; People suppress to plant the generation that the method for examining operation shellfish physiological vigor reduces " telling " nuclear phenomenon with the art pre-treatment; Use adhesive to bind the generation that technical measures such as wound, operation stitching wound stop " telling " nuclear phenomenon in recent years again, but effect is all undesirable.Have only the pearl nuclear of implanting internal sac to stay in the pearl shell body; Just can be formed with nucleus pearl; Therefore, how efficiently fixedly pearl nuclear, prevent that pearl nuclear " telling " from going out is that the great technical barrier that will solve is dreamed of by numerous scientific research personnel that are engaged in pearl culture and enterprise always.
Summary of the invention
The invention provides a kind of pressure that is used to culture nucleated pearl efficiently and stay the solid nuclear device of kernel method and use; Its purpose is to stay kernel method and use the present invention's solid nuclear device simple in structure, easy to use through the pressure of using the present invention's innovation, fundamentally solves " telling " nuclear issue of culturing in the large-scale nucleated pearl process.
For solving the problems of the technologies described above, the technical scheme that the present invention adopted is:
Culture the pressure of nucleated pearl and stay kernel method, include following steps:
A, clamp the pedestal of solid nuclear device at the shell edge of the pearl shell of implanting pearl nuclear; Perhaps before implanting pearl nuclear, the pedestal that utilizes adhesive will examine device admittedly is bonded on the shell of pearl shell;
B, the end that will examine the solid nuclear bar of device admittedly extend in the mantle cavity of pearl shell, and it is outside to be pressed in the internal sac of pearl nuclear position with certain pressure, will examine bar admittedly then and be fixed on the pedestal.
C, admittedly examine device and install after, place breeding water body to support temporarily pearl shell;
D, after the wound healing of the implantation pearl of pearl shell nuclear, unload solid nuclear device, pearl nuclear is forced to be stayed pearl shell and has suffered.
It is said that to examine device admittedly be by pedestal and examine bar admittedly constitutes; Said pedestal is by pedestal, fixedly steel disc, movable steel disc, screw bolt and nut constitute; Pedestal is the U-shaped structure; One sidewall of said U-shaped structure is provided with through hole, and the through hole is welded with nut, and the shell that the puller bolt that cooperates with nut passes from through hole pearl shell is fixed in the groove of pedestal; Said fixedly steel disc is located on the lateral surface of bottom of U-shaped structure, two bolts from movable steel disc and fixedly steel disc pass, admittedly examine that bar is located at movable steel disc and fixedly between the steel disc and between two bolts, and fix with nut.
Another sidewall of the pedestal of said U-shaped structure is provided with groove, be convenient to solid nuclear bar from then in the groove through and stretch in the pearl shell mantle cavity.
A said end of examining bar admittedly adopts elbow structure or Z-shaped structure, and this end is provided with the briquetting of band arcwall face, and the other end is provided with operating grip.
It is said that to examine device admittedly be by pedestal and examine bar admittedly constitutes; Said pedestal is by pedestal, fixedly steel disc, movable steel disc, screw bolt and nut constitute, and pedestal is to be made up of Z-shaped steel plate, and the plate face of Z-shaped steel plate can be bonded on the shell of pearl shell through adhesive; Said fixedly steel disc is located on the Z-shaped steel plate; Two bolts from movable steel disc and fixedly steel disc pass, admittedly examine that bar is located at movable steel disc and fixedly between the steel disc and between two bolts, and fix with nut.
Beneficial effect of the present invention: owing to adopt the technical measures of the mandatory fixedly pearl nuclear of external force, " telling " nuclear phenomenon can not take place in the pearl of being implanted nuclear; It is small and exquisite that the present invention examines the solid nuclear bar structure of device admittedly, in the use pearl shell growth, life had no adverse effects.The present invention fixedly method of pearl nuclear is simple, reliable for effect, plants nuclear shellfish core-remaining rate under the experimental condition and can reach 100%.
Description of drawings
Below in conjunction with accompanying drawing the present invention is done further detailed description.
Fig. 1 is a base construction sketch map in the embodiment of the invention 1;
Fig. 2 is a vertical view shown in Figure 1;
Fig. 3 is a upward view shown in Figure 1;
Fig. 4 is an end view shown in Figure 1;
Fig. 5 is a base construction sketch map in the embodiment of the invention 2;
Fig. 6 is a vertical view shown in Figure 5;
Fig. 7 is a upward view shown in Figure 5;
Fig. 8 is an end view shown in Figure 5;
Fig. 9 is the structural representation of the solid nuclear bar in the embodiment of the invention 1;
Figure 10 is the structural representation of the solid nuclear bar in the embodiment of the invention 2;
Figure 11 examines device overall structure sketch map admittedly for the present invention.
Among the figure, 1: base plate; 2: side plate; 3: upper plate; 4: fixing steel disc; 5: movable steel disc; 6: bolt; 7: fin neck bolt; 8: nut; 9: operating grip; 10: the body of rod; 11: briquetting; 12, groove.
Embodiment
Embodiment 1: constitute admittedly to examine device be by pedestal and examine bar admittedly.
Shown in Fig. 1-4; Said pedestal is by pedestal, fixedly steel disc 4, movable steel disc 5, bolt 6, fin neck bolt 7, nut 8 constitute; Pedestal is to be made up of base plate 1, side plate 2 and upper plate 3; Said base plate 1, side plate 2, upper plate 3 can be processed a U-shaped structure through cutting technologies such as pin, bending, polishing by a corrosion resistant plate, and fixedly steel disc 4 is welded on the side plate 2, make upper plate 2 inner faces and fixedly above the steel disc 4 at grade; In base plate 1, fixedly boring on steel disc 4, the movable steel disc 5; Again nut 8 is welded to base plate 1 and fixing steel disc 4 bore positions respectively, in the nut that is welded to base plate 18, is torqued into fin neck bolt 7, through the nut 8 on bolt 6 and the fixing steel disc 4 movable steel disc 5 and fixing steel disc 4 are coupled together again.
As shown in Figure 9, a said end of examining the body of rod 10 of bar admittedly adopts Z-shaped structure, and this end is provided with spherical briquetting 11, and the other end is provided with operating grip 9.
Utilize the pressure of the solid nuclear device breed nucleated pearl of embodiment 1 to stay kernel method, may further comprise the steps:
A, clamp the pedestal of solid nuclear device, and make that fixedly steel disc 4 is surperficial parallel with the shell inside edge at the shell edge of the pearl shell of implanting pearl nuclear;
B, shown in figure 11; Admittedly the briquetting of examining bar 11 ends are stretched in the large nacre mantle cavity; To examine club shaft 10 more admittedly places between the fixedly steel disc 4 and movable steel disc 5 centres and two bolts 6 on the pedestal through groove 12; Adjusting is examined bar admittedly and is stretched into the length in the pearl shell mantle cavity, makes briquetting 11 be pressed in the internal sac outside of pearl nuclear position; Appropriateness fastening bolt 6, making fixedly, steel disc 4 clamps the body of rod 10 of examining bar admittedly with movable steel disc 5 appropriateness; Turn the operating grip 9 of solid nuclear bar, make 11 pairs of internal sacs of briquetting of rod end form certain pressure; Further fastening bolt 6 makes solid nuclear bar stiff, remains certain pressure admittedly examine 11 pairs of pearls nuclear of the rod end briquetting position internal sac of bar, and the pearl of being implanted nuclear is fixed on the position of being implanted and can not " tells ".
C, admittedly examine device and install after, place breeding water body to support temporarily pearl shell;
D, culture after 10-15 days, at this moment, the wound of the implantation pearl of pearl shell nuclear heals basically, unloads solid nuclear device, and pearl nuclear is forced to be stayed pearl shell and suffered.
 
Embodiment 2: constitute admittedly to examine device be by pedestal and examine bar admittedly.
Shown in Fig. 5-8; Pedestal is by base plate 1, fixedly steel disc 4, movable steel disc 5, bolt 6, nut 8 constitute; Base plate 1, fixedly steel disc 4 can be processed through technologies such as cutting, bending, polishings by a corrosion resistant plate; Boring on fixing steel disc 4 and movable steel disc 5, and at the fixing bore position welded nut 8 of steel disc 4, again through the nut 8 on bolt 6 and the fixing steel disc 4 with movable steel disc 5 and fixedly steel disc 4 couple together.
Shown in figure 10, adopt elbow structure admittedly examine an end of the body of rod 10 of bar, this end is provided with sheet briquetting 11, and the other end is provided with operating grip 9.
Utilize the pressure of the solid nuclear device breed nucleated pearl of embodiment 2 to stay kernel method, may further comprise the steps:
A, before planting nuclear operation, with epoxy resin, water-borne glue etc. the base plate 1 of pedestal is bonded in the right shell of large nacre outside and is fit to the position, make that fixedly steel disc 4 is surperficial parallel with the shell inside edge;
B, briquetting 11 ends that will examine bar admittedly stretch in the large nacre mantle cavity; To examine bar more admittedly places between the fixedly steel disc 4 and movable steel disc 5 centres and two bolts 6 on the pedestal; Adjusting is examined bar admittedly and is stretched into the length in the pearl shell mantle cavity, makes briquetting 11 be pressed in the internal sac outside of pearl nuclear position; Appropriateness fastening bolt 6, making fixedly, steel disc 4 clamps the body of rod 10 of examining bar admittedly with movable steel disc 5 appropriateness; Turn the operating grip 9 of solid nuclear bar, make 11 pairs of internal sacs of briquetting form certain pressure; Further fastening bolt 6 makes solid nuclear bar stiff, remains certain pressure admittedly examine 11 pairs of pearls nuclear of the rod end briquetting position internal sac of bar, and the pearl of being implanted nuclear is fixed on the position of being implanted and can not " tells ".
C, admittedly examine device and install after, place breeding water body to support temporarily pearl shell;
D, culture after 10-15 days, at this moment, the wound of the implantation pearl of pearl shell nuclear heals basically, unloads solid nuclear device, and pearl nuclear is forced to be stayed pearl shell and suffered.
The above is a preferred implementation of the present invention, can not limit the present invention's interest field certainly with this.Should be pointed out that for those skilled in the art, technical scheme of the present invention is made amendment or is equal to replacement, do not break away from protection scope of the present invention.

Claims (6)

1. kernel method is stayed in a pressure of culturing nucleated pearl, it is characterized in that: include following steps: A, clamp the pedestal of solid nuclear device at the shell edge of the pearl shell of implanting pearl nuclear; Perhaps before implanting pearl nuclear, the pedestal that utilizes adhesive will examine device admittedly is bonded on the shell of pearl shell; B, the end that will examine the solid nuclear bar of device admittedly extend in the mantle cavity of pearl shell, and it is outside to be pressed in the internal sac of pearl nuclear position with certain pressure, will examine bar then admittedly and be fixed on the pedestal; C, admittedly examine device and install after, place breeding water body to support temporarily pearl shell; D, after the wound healing of the implantation pearl of pearl shell nuclear, unload solid nuclear device, pearl nuclear is forced to be stayed pearl shell and has suffered.
2. stay the used solid nuclear device of kernel method based on the pressure of the said breed nucleated pearl of claim 1; It is characterized in that: said to examine device admittedly be by pedestal and examine bar admittedly constitutes; Said pedestal is by pedestal, fixedly steel disc, movable steel disc, screw bolt and nut constitute, and pedestal is the U-shaped structure, and a sidewall of said U-shaped structure is provided with through hole; The through hole is welded with nut, and the shell that the puller bolt that cooperates with nut passes from through hole pearl shell is fixed in the groove of pedestal; Said fixedly steel disc is located on the lateral surface of bottom of U-shaped structure, two bolts from movable steel disc and fixedly steel disc pass, admittedly examine that bar is located at movable steel disc and fixedly between the steel disc and between two bolts, and fix with nut.
3. the device of examining admittedly according to claim 2 is characterized in that: another sidewall of the pedestal of said U-shaped structure is provided with groove.
4. the device of examining admittedly according to claim 2 is characterized in that: a said end of examining bar admittedly adopts elbow structure or Z-shaped structure, and this end is provided with the briquetting of band arcwall face, and the other end is provided with operating grip.
5. the device of examining admittedly according to claim 2 is characterized in that: said nut and fixedly steel disc be welded into an integral body.
6. stay the used solid nuclear device of kernel method according to the pressure of the said breed nucleated pearl of claim 1; It is characterized in that: said to examine device admittedly be by pedestal and examine bar admittedly constitutes; Said pedestal is by pedestal, fixedly steel disc, movable steel disc, screw bolt and nut constitute, and pedestal is to be made up of Z-shaped steel plate, and the plate face of Z-shaped steel plate can be bonded on the shell of pearl shell through adhesive; Said fixedly steel disc is located on the Z-shaped steel plate; Two bolts from movable steel disc and fixedly steel disc pass, admittedly examine that bar is located at movable steel disc and fixedly between the steel disc and between two bolts, and fix with nut.
CN2011103629187A 2011-11-16 2011-11-16 Forced nucleus retention method in nucleated pearl cultivation and nucleus retainer used in method Active CN102487848B (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN2011103629187A CN102487848B (en) 2011-11-16 2011-11-16 Forced nucleus retention method in nucleated pearl cultivation and nucleus retainer used in method
PCT/CN2012/076844 WO2013071746A1 (en) 2011-11-16 2012-06-13 Forced nucleus retention method in nucleated pear cultivation and nucleus retainer used in method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2011103629187A CN102487848B (en) 2011-11-16 2011-11-16 Forced nucleus retention method in nucleated pearl cultivation and nucleus retainer used in method

Publications (2)

Publication Number Publication Date
CN102487848A true CN102487848A (en) 2012-06-13
CN102487848B CN102487848B (en) 2013-12-04

Family

ID=46179767

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2011103629187A Active CN102487848B (en) 2011-11-16 2011-11-16 Forced nucleus retention method in nucleated pearl cultivation and nucleus retainer used in method

Country Status (2)

Country Link
CN (1) CN102487848B (en)
WO (1) WO2013071746A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103609499A (en) * 2013-12-02 2014-03-05 浙江海洋学院 Butterfly embracing type nucleus feeding device
CN103918582A (en) * 2013-01-10 2014-07-16 王俊杰 Pearl nucleus for pearl culture and production method thereof
CN106035154A (en) * 2016-06-04 2016-10-26 广东海洋大学 Forced nucleus-retaining method for culturing nucleated pearls

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20070070599A (en) * 2005-12-29 2007-07-04 정남진 흑진주 장흥키조개 영어조합법인 Tool for fixing pearl nucleus and cultivating method of black-pearl using the same
WO2007075064A1 (en) * 2005-12-29 2007-07-05 Jeongnamjin Heukjinju Jang Hung Kijoje Yeong Yeo Jo Hab Beob Yin Tool for fixing pearl nucleus and method for cultivating black pearl using the same
CN101253849A (en) * 2008-03-11 2008-09-03 广东海洋大学 Method for breeding blister pearl using magnetic attractive force to fix bead nuclei
JP2008237187A (en) * 2007-03-29 2008-10-09 Meiko Pearl Ushiku Kanko Kk Nucleus for pearl, method for producing the same, and pearl
CN101849521A (en) * 2010-06-24 2010-10-06 雷州市宝贝珍珠养殖有限公司 Nucleus inserting and pearl culturing method for self-absorbing pearl nucleus of pearl shell

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0819349A (en) * 1994-07-06 1996-01-23 Mitsuo Horiguchi Pearl seed inoculation structure in pearl culture
JP4378542B2 (en) * 2003-08-22 2009-12-09 学校法人日本大学 Mother shell cutting method and apparatus
US7404378B2 (en) * 2006-02-17 2008-07-29 Batzer William B Pearl culture method and product
CN102160530B (en) * 2011-04-28 2012-12-26 广东海洋大学 Method for culturing nucleated pearls by using magnetic attraction fixed pearl nucleuses

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20070070599A (en) * 2005-12-29 2007-07-04 정남진 흑진주 장흥키조개 영어조합법인 Tool for fixing pearl nucleus and cultivating method of black-pearl using the same
WO2007075064A1 (en) * 2005-12-29 2007-07-05 Jeongnamjin Heukjinju Jang Hung Kijoje Yeong Yeo Jo Hab Beob Yin Tool for fixing pearl nucleus and method for cultivating black pearl using the same
JP2008237187A (en) * 2007-03-29 2008-10-09 Meiko Pearl Ushiku Kanko Kk Nucleus for pearl, method for producing the same, and pearl
CN101253849A (en) * 2008-03-11 2008-09-03 广东海洋大学 Method for breeding blister pearl using magnetic attractive force to fix bead nuclei
CN101849521A (en) * 2010-06-24 2010-10-06 雷州市宝贝珍珠养殖有限公司 Nucleus inserting and pearl culturing method for self-absorbing pearl nucleus of pearl shell

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
郑俊英: "淡水有核珍珠的固核措施及其存在问题", 《淡水渔业》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103918582A (en) * 2013-01-10 2014-07-16 王俊杰 Pearl nucleus for pearl culture and production method thereof
CN103609499A (en) * 2013-12-02 2014-03-05 浙江海洋学院 Butterfly embracing type nucleus feeding device
CN106035154A (en) * 2016-06-04 2016-10-26 广东海洋大学 Forced nucleus-retaining method for culturing nucleated pearls

Also Published As

Publication number Publication date
CN102487848B (en) 2013-12-04
WO2013071746A1 (en) 2013-05-23

Similar Documents

Publication Publication Date Title
Gémes-Juhász et al. Effect of optimal stage of female gametophyte and heat treatment on in vitro gynogenesis induction in cucumber (Cucumis sativus L.)
RU2010138801A (en) METHODS, SURFACE-MODIFIED CARRIERS AND COMPOSITIONS FOR IMMOBILIZATION, CULTIVATION AND FASTENING OF CELLS
CN102487848B (en) Forced nucleus retention method in nucleated pearl cultivation and nucleus retainer used in method
CN102067823B (en) Buoyant-raft net cage sea cucumber culture device and sea cucumber culture method
CN204676512U (en) A kind of aquaculture floater collecting device
WO2019061560A1 (en) In vitro maturation culture medium of immature oocytes and use thereof
CN208972169U (en) A kind of forestry nursery stock transplanting fixed frame
CN204047598U (en) Combined type deep water mesh cage
CN204948858U (en) Fixing device based on forest grafting
CN102499193A (en) Near-shore cultivating and breeding method and device suitable for dendroidal Hermatypic coral
CN204837561U (en) Broadcast breed cage at bottom of scallop of luxurious comb hole
CN111316943A (en) Pond seedling raising method for ostrea rivularis
CN201299040Y (en) Aquatic animal breeding container with vertical-clapboard fixer
CN205320548U (en) Automatic transplanting equipment is cultivated to plant
CN213245905U (en) Economic forest nursery stock breeding device
CN108834968B (en) Shallow sea attached shellfish culture and proliferation device and using method thereof
CN206150035U (en) Trees heel in structure
CN201617093U (en) Offshore abalone hanging culture device
CN103636547B (en) Method for acquiring mature eggs of aurelia
KR101847789B1 (en) Rope fastener for aquaculture of seaweed
CN101248772A (en) Breeding method of fresh water genuine pearl with pearl
CN115362964B (en) Method for culturing Pinctada maxima Bei Shenyuan sea
CN209283839U (en) A kind of mausoleum party device for raising seedlings convenient for transplanting
CN104430096B (en) A kind of Siniperca chuatsi family selective breeding group isolation hatching method
Florin et al. In vitro cryopreservation of cacao genetic resources

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant