CN102449168A - 基因毒性测试 - Google Patents

基因毒性测试 Download PDF

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Publication number
CN102449168A
CN102449168A CN2010800231330A CN201080023133A CN102449168A CN 102449168 A CN102449168 A CN 102449168A CN 2010800231330 A CN2010800231330 A CN 2010800231330A CN 201080023133 A CN201080023133 A CN 201080023133A CN 102449168 A CN102449168 A CN 102449168A
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CN
China
Prior art keywords
gluc
cell
cells
assay
expression cassette
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Pending
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CN2010800231330A
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English (en)
Chinese (zh)
Inventor
A.拉比诺维茨
R.瓦尔姆斯利
M.塔特
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Gentronix Ltd
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Gentronix Ltd
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Application filed by Gentronix Ltd filed Critical Gentronix Ltd
Publication of CN102449168A publication Critical patent/CN102449168A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN2010800231330A 2009-03-28 2010-03-26 基因毒性测试 Pending CN102449168A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0905410.7 2009-03-28
GBGB0905410.7A GB0905410D0 (en) 2009-03-28 2009-03-28 Genotoxicity testing
PCT/GB2010/000581 WO2010112821A1 (en) 2009-03-28 2010-03-26 Genotoxicity testing

Publications (1)

Publication Number Publication Date
CN102449168A true CN102449168A (zh) 2012-05-09

Family

ID=40671920

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2010800231330A Pending CN102449168A (zh) 2009-03-28 2010-03-26 基因毒性测试

Country Status (8)

Country Link
US (2) US20120035079A1 (enExample)
EP (1) EP2411540A1 (enExample)
JP (1) JP2012524523A (enExample)
CN (1) CN102449168A (enExample)
AU (1) AU2010231209A1 (enExample)
CA (1) CA2755985A1 (enExample)
GB (1) GB0905410D0 (enExample)
WO (1) WO2010112821A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102851388A (zh) * 2012-09-29 2013-01-02 山东大学齐鲁医院 一种用于鉴别卵巢癌易感性的分子标记、试剂盒及其鉴别方法
CN102864234A (zh) * 2012-09-29 2013-01-09 山东大学齐鲁医院 一种用于鉴别卵巢癌易感性的分子标记
CN104364649A (zh) * 2012-06-13 2015-02-18 默克专利股份有限公司 用于鉴定具有基因毒性的化合物的蛋白表达分析
CN105200069A (zh) * 2015-10-16 2015-12-30 北京交通大学 基于rna聚合酶ⅰ的rsv微型复制基因组及其应用

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9285358B2 (en) * 2011-04-08 2016-03-15 Anthem Biosciences Pvt Ltd Vitro method for high throughput screening of genotoxic agents in eukaryotic cells
US11307205B2 (en) 2016-09-19 2022-04-19 University Of Southern California Non-radioactive cytotoxicity assays
SG11202003885UA (en) 2017-11-08 2020-05-28 Twinstrand Biosciences Inc Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
AU2019221549C1 (en) * 2018-02-13 2025-11-27 Twinstrand Biosciences, Inc. Methods and reagents for detecting and assessing genotoxicity
SG11202100141SA (en) 2018-07-12 2021-02-25 Twinstrand Biosciences Inc Methods and reagents for characterizing genomic editing, clonal expansion, and associated applications
CN119955899A (zh) * 2018-10-03 2025-05-09 普洛麦格公司 用于稳定腔肠素及其类似物和衍生物的组合物、方法和试剂盒
CN114606269B (zh) * 2022-03-17 2024-02-23 国家食品安全风险评估中心 一种用于食品接触材料迁移物的遗传毒性的高通量检测方法及应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005113802A2 (en) * 2004-05-20 2005-12-01 Gentronix Limited Genotoxic testing
CN101149327A (zh) * 2007-11-06 2008-03-26 浙江大学 基于细胞显微图像信息的抗肿瘤药物评价和筛选方法
CN101322033A (zh) * 2005-09-26 2008-12-10 乌尔里希·洛斯 用于检测针对tsh受体的自身免疫抗体的方法和新型tsh受体嵌合体

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005113802A2 (en) * 2004-05-20 2005-12-01 Gentronix Limited Genotoxic testing
CN101322033A (zh) * 2005-09-26 2008-12-10 乌尔里希·洛斯 用于检测针对tsh受体的自身免疫抗体的方法和新型tsh受体嵌合体
CN101149327A (zh) * 2007-11-06 2008-03-26 浙江大学 基于细胞显微图像信息的抗肿瘤药物评价和筛选方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
TANNOUS等: "Codon-Optimized Gaussia Luciferase cDNA for Mammalian Gene Expression in Culture and in Vivo", 《MOLECULAR THERAPY》 *
曹永献等: "流式细胞仪计数网织红细胞方法评价", 《检验医学》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104364649A (zh) * 2012-06-13 2015-02-18 默克专利股份有限公司 用于鉴定具有基因毒性的化合物的蛋白表达分析
CN104364649B (zh) * 2012-06-13 2017-05-17 默克专利股份有限公司 用于鉴定具有基因毒性的化合物的蛋白表达分析
CN102851388A (zh) * 2012-09-29 2013-01-02 山东大学齐鲁医院 一种用于鉴别卵巢癌易感性的分子标记、试剂盒及其鉴别方法
CN102864234A (zh) * 2012-09-29 2013-01-09 山东大学齐鲁医院 一种用于鉴别卵巢癌易感性的分子标记
CN102851388B (zh) * 2012-09-29 2014-03-19 山东大学齐鲁医院 一种用于鉴别卵巢癌易感性的分子标记、试剂盒及其鉴别方法
CN105200069A (zh) * 2015-10-16 2015-12-30 北京交通大学 基于rna聚合酶ⅰ的rsv微型复制基因组及其应用

Also Published As

Publication number Publication date
US20120035079A1 (en) 2012-02-09
EP2411540A1 (en) 2012-02-01
WO2010112821A1 (en) 2010-10-07
CA2755985A1 (en) 2010-10-07
JP2012524523A (ja) 2012-10-18
AU2010231209A1 (en) 2011-10-06
GB0905410D0 (en) 2009-05-13
US20140302512A1 (en) 2014-10-09

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Application publication date: 20120509