CN102440235B - Method for preserving pollen of asteraceae and allied plants - Google Patents
Method for preserving pollen of asteraceae and allied plants Download PDFInfo
- Publication number
- CN102440235B CN102440235B CN2011102605202A CN201110260520A CN102440235B CN 102440235 B CN102440235 B CN 102440235B CN 2011102605202 A CN2011102605202 A CN 2011102605202A CN 201110260520 A CN201110260520 A CN 201110260520A CN 102440235 B CN102440235 B CN 102440235B
- Authority
- CN
- China
- Prior art keywords
- pollen
- chrysanthemum
- preserving
- activity
- artemisia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 21
- 241000196324 Embryophyta Species 0.000 title claims abstract description 16
- 241000208838 Asteraceae Species 0.000 title abstract 3
- 241000723353 Chrysanthemum Species 0.000 claims abstract description 47
- 235000007516 Chrysanthemum Nutrition 0.000 claims abstract description 47
- 230000000694 effects Effects 0.000 claims abstract description 25
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000004321 preservation Methods 0.000 claims description 22
- 240000000073 Achillea millefolium Species 0.000 claims description 5
- 235000007754 Achillea millefolium Nutrition 0.000 claims description 5
- 241000271889 Artemisia japonica Species 0.000 claims description 5
- 235000017521 Artemisia japonica Nutrition 0.000 claims description 5
- 235000007871 Chrysanthemum coronarium Nutrition 0.000 claims description 5
- 244000067456 Chrysanthemum coronarium Species 0.000 claims description 5
- 229920001030 Polyethylene Glycol 4000 Polymers 0.000 claims description 5
- 240000004460 Tanacetum coccineum Species 0.000 claims description 5
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 claims description 5
- 230000007198 pollen germination Effects 0.000 claims description 5
- 238000005070 sampling Methods 0.000 claims description 5
- 230000000007 visual effect Effects 0.000 claims description 5
- 241000828937 Ajania salicifolia Species 0.000 claims description 4
- 240000006891 Artemisia vulgaris Species 0.000 claims description 4
- 235000017371 Persisch Vlokruid Nutrition 0.000 claims description 4
- 241000404034 Ajania Species 0.000 claims 1
- 241000404033 Ajania fruticulosa Species 0.000 claims 1
- 241001201008 Artemisia sieversiana Species 0.000 claims 1
- 235000004040 Artemisia sieversiana Nutrition 0.000 claims 1
- 235000008495 Chrysanthemum leucanthemum Nutrition 0.000 claims 1
- 244000035851 Chrysanthemum leucanthemum Species 0.000 claims 1
- 244000192528 Chrysanthemum parthenium Species 0.000 claims 1
- 235000000604 Chrysanthemum parthenium Nutrition 0.000 claims 1
- 241000404166 Mauranthemum paludosum Species 0.000 claims 1
- 238000002955 isolation Methods 0.000 abstract description 6
- 241000894007 species Species 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 238000001514 detection method Methods 0.000 abstract description 4
- 238000009395 breeding Methods 0.000 abstract 2
- 230000001488 breeding effect Effects 0.000 abstract 2
- 238000009402 cross-breeding Methods 0.000 description 4
- 230000035899 viability Effects 0.000 description 4
- 238000001035 drying Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 3
- 238000009396 hybridization Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 241000722941 Achillea Species 0.000 description 1
- 235000003826 Artemisia Nutrition 0.000 description 1
- 235000003261 Artemisia vulgaris Nutrition 0.000 description 1
- 240000005250 Chrysanthemum indicum Species 0.000 description 1
- 235000018959 Chrysanthemum indicum Nutrition 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 244000030166 artemisia Species 0.000 description 1
- 235000009052 artemisia Nutrition 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for preserving pollen of asteraceae and allied plants, belonging to the field of hybridizing and breeding of chrysanthemums. The method comprises the following steps of: putting collected fresh pollen in a tinfoil paper parcel after airing for 1-2 h, preserving the pollen at -80-4 DEG C, and carrying out pollen activity detection one time every two months so as to evaluate the preserving activity of the pollen. Aiming at the asteraceae and allied wild species, a scientific and simple pollen preserving method is provided; the method is beneficial to developing the hybridizing and breeding work without time difference and geographic isolation; furthermore, the preserving temperature most suitable for maintaining the activity of the pollen is explored.
Description
Technical field
The present invention relates to chrysanthemum crossbreeding field, relate in particular to a kind of method for preserving of Chrysanthemum relative genus plant pollen.
Background technology
Chrysanthemum Origin, in China, at China's cultivation history of existing more than 2000 year, is not only one of Chinese ten great tradition famous flowers, and is one of the large cut-flower in the world four and ten large potted flowers, has become one of most important flower plant in the world flower industry.The chrysanthemum cultivar is various, pattern is abundant, but also there is certain deficiency at aspects such as fancy points such as Resistant, heat resistanceheat resistant, the resistance trait such as cold-resistant and plant types, cause serious its output, quality and the productivity effect of having reduced, also limited it and applied territorial scope simultaneously.The chrysanthemum sibling species belongs in wild plant and has the merit that many cultivating chrysanthemums lack, disease and insect resistance as camomile, mother chrysanthemum etc., cover the drought resistance of chrysanthemum, the warm tolerance of chrysanthemum brain and the cold resistance of A Erju etc., therefore by middle Crossing system, these merits being imported to the cultivation chrysanthemum is an important channel of carrying out chrysanthemum germplasm innovation and breed improvement.
Yet most of Chrysanthemum relative genus wild species and cultivating chrysanthemum distant hybridization exist flowering asynchronism and geographic isolation problem.As shown in table 1, the natural florescence of cultivating chrysanthemum, normally July, and the majority of plants such as inferior Chrysanthemum, a Chrysanthemum, artemisia and Achillea nature was April to June at the florescence, causes flowering asynchronism, further forms the reproduction isolation to November.In Chrysanthemum and Related species plant thereof, the Taihang Chrysanthemum is confined to central China, thereby causes the geographic isolation between not belonging to together.It is one of important channel solved flowering asynchronism and geographic isolation problem that pollen is preserved.But the pollen of Chrysanthemum and relative genus plant thereof is under general condition preserved, pollen viability there will be significantly decline, is difficult to for the research that is cross-breeding.
Good characteristic, distribution and the florescence of 10 Chrysanthemum relative genus wild species of table 1
Summary of the invention
Goal of the invention: the purpose of this invention is to provide a kind of method for preserving of Chrysanthemum relative genus plant pollen, to overcoming the obstacle in chrysanthemum hybridization, realize the transfer of various excellent genes between different Chrysanthemums.
Technical scheme: for achieving the above object, the method for preserving of a kind of Chrysanthemum relative genus plant pollen of the present invention comprises: the fresh pollen airing of collection is put into to the tinfoil paper paper bag after 1～2 hour, in-80～4 ℃ of lower preservations.
Within every two months, carry out a Pollen Activity and detect, described Pollen Activity detects and comprises: the pollen sampling is put into to ME3+200gL
-1in the PEG4000 medium, under 20 ℃ of dark conditions, cultivate 2～10h, 10 visuals field of random observation under the microscope, if pollen tube length is greater than pollen, diameter is considered as pollen germination.
Described pollen acquisition time should be between the 10:00～14:00 of fine day.
As preferred version of the present invention, the polliniferous tinfoil paper paper bag of described bag is placed in-25～-15 ℃ of lower low-temperature preservations.As optimal case of the present invention, described preservation temperature is-20 ℃.
But adopt the pollen of the various Chrysanthemums of method preservation provided by the invention and relative genus wild species thereof, in crossbreeding work, generally introduce the various resistances that resistant gene strengthens chrysanthemum, comprise drought resisting, cold-resistant, pest-resistant etc., as cited as above-mentioned table 1.
Beneficial effect: the method for preserving of a kind of Chrysanthemum relative genus plant pollen of the present invention is for Chrysanthemum and relative genus wild species thereof, science, simple pollen method for preserving are provided, be beneficial to crossbreeding work without the time difference, without carrying out on geographic isolation ground, and explore and be beneficial to the preservation temperature that maintains pollen activity most.
Embodiment
Embodiment 1
Gather respectively the fresh pollen of Ajania salicifolia (Mattf.) Poljak, the inferior chrysanthemum of shrub, Artemisia japonica, large seed wormwood artemisia, crowndaisy chrysanthemum, Bai Jingju, chrysanthemum coccineum, " Duanshepizu ", shore chrysanthemum, 10 Chrysanthemum relative genus of Achillea millefolium, acquisition time belongs to kind of a corresponding florescence for each, selects the fine day between 10:00～14:00.
The fresh pollen airing gathered is guaranteed to its drying after 1～2 hour, then get the part fresh pollen and carry out vitality test, all the other fresh pollen are put into the tinfoil paper paper bag, will wrap polliniferous tinfoil paper paper bag and be placed in 4 ℃ of lower low-temperature preservations, detected pollen viability, METHOD FOR CONTINUOUS DETERMINATION 3 times every 2 months.
The detection of Pollen Activity is specific as follows: the pollen sampling is put into to ME3+200gL
-1in the PEG4000 medium, under 20 ℃ of dark conditions, cultivate 2～10h, 10 visuals field of random observation under the microscope, if pollen tube length is greater than pollen, diameter is considered as pollen germination.
Above-mentioned pollen, after preservation, detects respectively Pollen Activity every other month every 2, and METHOD FOR CONTINUOUS DETERMINATION 3 times adds up as shown in table 2:
The Pollen Activity of 4 ℃ of lower pollen preservations that table 2 was measured every February
Annotate: the table intermediate value is that mean value adds the standard error, and the female representative of upper right corner institute marking-up is through the significant difference (p≤0.05) of Turkey check
As can be seen from the above table, carry out its Pollen Activity of pollen preservation under 4 ℃ and descend comparatively fast, the Pollen Activity that preservation 1～2 month is most of to be belonged to kind descends not remarkable, but still has part to belong to kind 4 ℃ of lower preservations after 2 months, and Pollen Activity descends more obvious.
Embodiment 2
Gather respectively the fresh pollen of Ajania salicifolia (Mattf.) Poljak, the inferior chrysanthemum of shrub, Artemisia japonica, large seed wormwood artemisia, crowndaisy chrysanthemum, Bai Jingju, chrysanthemum coccineum, " Duanshepizu ", shore chrysanthemum, 10 Chrysanthemum relative genus of Achillea millefolium, acquisition time belongs to kind of a corresponding florescence for each, selects the fine day between 10:00～14:00.
The fresh pollen airing gathered is guaranteed to its drying after 1～2 hour, then get the part fresh pollen and carry out vitality test, all the other fresh pollen are put into the tinfoil paper paper bag, will wrap polliniferous tinfoil paper paper bag and be placed in-20 ℃ of lower low-temperature preservations, detected pollen viability, METHOD FOR CONTINUOUS DETERMINATION 3 times every 2 months.
The detection of Pollen Activity is specific as follows: the pollen sampling is put into to ME3+200gL
-1in the PEG4000 medium, under 20 ℃ of dark conditions, cultivate 2～10h, 10 visuals field of random observation under the microscope, if pollen tube length is greater than pollen, diameter is considered as pollen germination.
Above-mentioned pollen, after preservation, detects respectively Pollen Activity every other month every 2, and METHOD FOR CONTINUOUS DETERMINATION 3 times adds up as shown in table 3:
The Pollen Activity of-20 ℃ of lower pollen preservations that table 3 was measured every February
Annotate: the table intermediate value is that mean value adds the standard error, and the female representative of upper right corner institute marking-up is through the significant difference (p≤0.05) of Turkey check
As can be seen from the above table, carry out its Pollen Activity of pollen preservation under-20 ℃ and have remarkable improvement, Pollen Activity descends hardly, and the preservation time can be greater than 6 months.
Embodiment 3
Gather respectively the fresh pollen of Ajania salicifolia (Mattf.) Poljak, the inferior chrysanthemum of shrub, Artemisia japonica, large seed wormwood artemisia, crowndaisy chrysanthemum, Bai Jingju, chrysanthemum coccineum, " Duanshepizu ", shore chrysanthemum, 10 Chrysanthemum relative genus of Achillea millefolium, acquisition time belongs to kind of a corresponding florescence for each, selects the fine day between 10:00～14:00.
The fresh pollen airing gathered is guaranteed to its drying after 1～2 hour, then get the part fresh pollen and carry out vitality test, all the other fresh pollen are put into the tinfoil paper paper bag, will wrap polliniferous tinfoil paper paper bag and be placed in-80 ℃ of lower low-temperature preservations, detected pollen viability, METHOD FOR CONTINUOUS DETERMINATION 3 times every 2 months.
The detection of Pollen Activity is specific as follows: the pollen sampling is put into to ME3+200gL
-1in the PEG4000 medium, under 20 ℃ of dark conditions, cultivate 2～10h, 10 visuals field of random observation under the microscope, if pollen tube length is greater than pollen, diameter is considered as pollen germination.
Above-mentioned pollen, after preservation, detects respectively Pollen Activity every other month every 2, and METHOD FOR CONTINUOUS DETERMINATION 3 times adds up as shown in table 4:
The Pollen Activity of-80 ℃ of lower pollen preservations that table 4 was measured every February
Annotate: the table intermediate value is that mean value adds the standard error, and the female representative of upper right corner institute marking-up is through the significant difference (p≤0.05) of Turkey check
As can be seen from the above table, carry out pollen preservation Pollen Activity under-80 ℃ and just descended significantly when preserving 2 months, subsequently 4 the middle of the month Pollen Activity there is no marked change, therefore-80 ℃ are unfavorable for the pollen preservation.
The above is only the preferred embodiment of the present invention; be noted that for those skilled in the art; under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (2)
1. the method for preserving of a Chrysanthemum relative genus plant pollen, is characterized in that, the method comprises: the fresh pollen airing gathered is put into to the tinfoil paper paper bag after 1 ~ 2 hour, in-20 ℃ of lower preservations;
Described pollen is taken from any one of following Chrysanthemum relative genus plant: Ajania salicifolia (Mattf.) Poljak (
ajania salicifoli)
,the inferior chrysanthemum of shrub (
ajania fruticulosa), Artemisia japonica (
artemisia japonica)
,large seed wormwood artemisia (
artemisia sieversiana)
,crowndaisy chrysanthemum (
chrysanthemum coronarium)
,bai Jingju (
chrysanthemum paludosum)
,chrysanthemum coccineum (
pyrethrum coccineum)
," Duanshepizu " (
pyrethrum parthenium)
,the shore chrysanthemum (
leucanthemum vulgare)
,achillea millefolium (
achillea millefolium).
2. the method for preserving of a kind of Chrysanthemum relative genus plant pollen according to claim 1 is characterized in that: within every two months, carry out a Pollen Activity and detect, described Pollen Activity detects and comprises: the pollen sampling is put into to ME3+200gL
-1in the PEG4000 medium, under 20 ℃ of dark conditions, cultivate 2 ~ 10h, 10 visuals field of random observation under the microscope, if pollen tube length is greater than pollen, diameter is considered as pollen germination.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102605202A CN102440235B (en) | 2011-09-05 | 2011-09-05 | Method for preserving pollen of asteraceae and allied plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102605202A CN102440235B (en) | 2011-09-05 | 2011-09-05 | Method for preserving pollen of asteraceae and allied plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102440235A CN102440235A (en) | 2012-05-09 |
| CN102440235B true CN102440235B (en) | 2013-12-11 |
Family
ID=46003563
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011102605202A Expired - Fee Related CN102440235B (en) | 2011-09-05 | 2011-09-05 | Method for preserving pollen of asteraceae and allied plants |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102440235B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104351170B (en) * | 2014-09-23 | 2017-08-08 | 江苏省林业科学研究院 | A kind of shrub willow pollen store method |
-
2011
- 2011-09-05 CN CN2011102605202A patent/CN102440235B/en not_active Expired - Fee Related
Non-Patent Citations (8)
| Title |
|---|
| 吕晋慧等.地被菊花粉生活力测定及影响因素研究.《华北农学报》.2011,第26卷(第4期),第189-193页. |
| 地被菊花粉生活力测定及影响因素研究;吕晋慧等;《华北农学报》;20110828;第26卷(第4期);第189-193页 * |
| 地被菊花粉萌发及花粉活力的研究;毛洪玉等;《辽宁林业科技》;20040930(第5期);第12-13页 * |
| 小菊花粉生活力及贮藏力的研究;王涛;《辽宁林业科技》;20100115(第1期);摘要，第9页第1栏1.2.2花粉贮藏与活力测定 * |
| 栽培小菊和几种菊属植物花粉离体萌发研究;赵宏波等;《南京农业大学学报》;20050630;第28卷(第2期);摘要，第23页1.2.2试验处理部分 * |
| 毛洪玉等.地被菊花粉萌发及花粉活力的研究.《辽宁林业科技》.2004,(第5期),第12-13页. |
| 王涛.小菊花粉生活力及贮藏力的研究.《辽宁林业科技》.2010,(第1期),第8-11页. |
| 赵宏波等.栽培小菊和几种菊属植物花粉离体萌发研究.《南京农业大学学报》.2005,第28卷(第2期),第22-27页. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102440235A (en) | 2012-05-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101803564B (en) | Simplified breeding method for preparing hybrid cabbage by using cytoplasmic male sterile lines | |
| Zhao et al. | Chrysanthemum genetic resources and related genera of Chrysanthemum collected in China | |
| CN102960146B (en) | Planting method for planting eco-economic vegetation in gentle slope land of karst stony desertification region | |
| CN103314840B (en) | 'Three-selection, two-advancing and one-promotion' apple breeding method | |
| CN104304238B (en) | The Embedding drying cryopreservation method of downy grape stem apex | |
| CN106212269A (en) | The method of the Herba Alii fistulosi new varieties of the selection-breeding high yield and high quality of resistance to storage | |
| Zhong et al. | The breeding progress and development status of the kiwifruit industry in China | |
| CN101697707B (en) | Method for cultivating amomum villosum | |
| CN102440235B (en) | Method for preserving pollen of asteraceae and allied plants | |
| CN105961377A (en) | Castanopsis hystrix pollen collecting and storing method for increasing germination rate and improving pollen viability | |
| CN103098705A (en) | Study on breeding and cultivation technology of butterfly orchid variety | |
| CN105340560A (en) | Introduction and cultivation method of selaginella tamariscina | |
| CN102986400B (en) | Domestication method of cold-resistant vetiver grass | |
| CN104206266A (en) | Method for rapidly screening restorer of pepper yellow sprout cytoplasm male sterile line | |
| Chen et al. | Tea plant (Camellia sinensis) breeding in Korea | |
| Tel-Zur | Vine cacti (Hylocereus species): an emerging fruit crop | |
| Pal et al. | Evaluation of double whorled balsam (Impatiens balsamina L.) genotypes for growth, flowering and seed attributes | |
| CN104365383A (en) | New grape variety cultivation method | |
| CN104521747B (en) | The screening of a kind of high temperature resisting cotton and authentication method | |
| CN101015272B (en) | Method for breeding novel varieties of mellow-type ground-cover chrysanthemum utilizing D.indicum var.aromaticum | |
| Dhiman et al. | Lilium: Conservation, characterization, and evaluation | |
| Singh et al. | Assessment of African marigold (Tagetes erecta) genotypes in Uttarakhand | |
| CN103535278B (en) | Production method of cockscomb tube flower | |
| Bisht et al. | Studies on flowering behaviour of double type varieties of African marigold (Tagetes erecta L.) in different seasons under Uttarakhand conditions. | |
| CN102630568A (en) | Gomphrena globosa test tube flower and culture method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131211 |