CN104521747B - The screening of a kind of high temperature resisting cotton and authentication method - Google Patents
The screening of a kind of high temperature resisting cotton and authentication method Download PDFInfo
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Abstract
The screening of a kind of high temperature resisting cotton and authentication method, for high temperature resisting cotton Germplasms and the screening of kind and qualification.By nutrition bowl seedling transplanting or seed sowing mode in field planting cotton material, process at the mepiquat chloride that stem leaf length to 10～12 leaf period concentrations is 80～100mg/L, after process 18～20d time determine strain measure plant height and limit stem internode length, then according to plant height average rate of increase and restriction stem internode average length, detected materials is divided into high temperature resistant and sensitive two types.The screening of high temperature resisting cotton of the present invention and authentication method, it is not necessary to chamber facility provides High Temperature Stress, cost-effective, simple to operate, and evaluation criterion is directly perceived, reliable results, and energy Rapid identification in early days, have significant application value in the high temperature resistant breeding of Cotton Gossypii on producing.
Description
Technical field
The present invention relates to screening and the authentication method of a kind of high temperature resisting cotton, be exclusively used in high temperature resisting cotton Germplasms
Screening with kind and qualification, belong to crops breeding for stress tolerance field.
Background technology
Global warming has become people to know together.It is expected that, to this century inferior lobe global average temperature will raise 1.4～
5.8 DEG C, and the generation of extreme climate such as high temperature, arid etc. will be more frequent.The rising of the average annual temperature of China slightly above whole world
Heating 0.5-0.8 DEG C, following summer (6 to August) occurs that the probability of thermal extremes is up to more than 90% (Battisti D S
and Naylor R L.Historical Warnings of Future Food Insecurity with
Unprecedented Seasonal Heat.Science,2009,323:240-244).At present, more than 35 during 7 to August
DEG C seasonal high temperature take place frequently at Cotton in China planting area, now Cotton Gossypii is in and blooms and tie the bell phase, high temperature stress affect
Pollen development, pollen fertility reduces even abortion, causes a large amount of fruit abscission rate, thus has a strong impact on the formation of yield and quality.
Selection-breeding refractory wool flower variety is to adapt to when planting in advance cotton environment and the weather conditions that warm in the future are to stabilize and increase cotton
The effective way of flower yield and quality, and set up simplicity, high temperature resisting cotton screening accurately and authentication method, and acquisition has resistance to
The cotton material of hot properties is premise and the basis of high temperature resisting cotton breeding.Owing to Cotton Gossypii has indeterminate growth, flowering habit
Stronger compensation ability, thus can not be resistance to as high temperature using the most solid (bell) rate and yield traits as the crops such as Oryza sativa L.
The screening index of property.In early-stage Study, we combine indoor pollen cultures and field cultivation test, by external pollen germination rate,
Pollen tube growth length and flowering and boll-setting period tie bell characteristic screening index (Zhi Liu (Liu Zhi), You-Lu as high temperature resisting cotton
Yuan,Shao-Qing Liu,Xiao-Nan Yu and Li-Qun Rao.Screening for High-Temperature
Tolerant Cotton Cultivars by Testing in vitro Pollen Germination,Pollen Tube
Growth and Boll Retention.Journal of Integrative Plant Biology,2006,48(6):
706-714), and propose the selection (ZL201210073660.3) of a kind of high temperature resisting cotton hybrid.This screening technique
Need not chamber facility and provide High Temperature Stress, operation is relatively simple, and accuracy is high, but needs pollen cultures and knot bell to adjust during screening
Looking into, the whole screening cycle is longer, in addition it is also necessary to certain data handling procedure, and screening technique is the most directly perceived.Recently, Chinese invention is special
Profit " a kind of method for rapid identification of heat-resistance seedling state of cotton " (application number: 201210041026.1) disclosed in Cotton Gossypii 3 to the 4 leaf phase
After processing 5～12h with 40～45 DEG C, by calculating ascorbate peroxidase enzymatic activity, relative conductivity and seedling wilted percent
Relative and normal growth relative value determines the method for the thermostability of Cotton Gossypii.Though the method can resistance to cotton material in early days
Hot identify, but in early days seedling due to own growth fragile, physio-biochemical characteristics such as are not given full play at the factor, difficult
True resistance to sex expression with showing material；It addition, measuring of the indexs such as Physiology and biochemistry needs relevant instrument and equipment condition, count
According to reproducibility and reliability the most poor, thus have impact on the accuracy of the operability of the method, practicality and qualification result.
Another Chinese invention patent " a kind of resistant to elevated temperatures screening technique of field test Cotton Gossypii " (application number: 201410079760.6) is open
The artificial high-temperature process in field, by indoor observation with calculate pollen germination rate, it is judged that the method for the heat-resisting quantity of Cotton Gossypii.Should
Method needs to build simple high temperature canopy at Cotton Gossypii initial bloom stage and carries out artificial high temperature stress process, and high-temperature process is difficult to control, and is easily subject to
Natural environment variations in temperature and the impact of other conditions, and pollen germination test is the most rigorous, it is judged that and standard is fuzzyyer.
Summary of the invention
Present invention aims to the deficiencies in the prior art, it is provided that the screening of a kind of high temperature resisting cotton and qualification side
Method, with screening and the High Temperature Stress patience of evaluation cotton material, this invention is easy and simple to handle, and evaluation criterion is directly perceived, reliable results, and
Rapid screening and the effect of qualification in early days can be reached.
The screening of a kind of high temperature resisting cotton and authentication method, comprise the following steps:
(1) field planting: use nutrition bowl seedling transplanting or seed sowing mode field planting material to be screened；(2) contracting
Joint amine processes: carry out mepiquat chloride process in cotton seedling stem leaf length to 8～12 leaf periods；
(3) determine strain measure plant height and limit the length of stem internode: on the same day carrying out mepiquat chloride process, wait to sieve from every part
Selecting the individual plant that at least 10% growth and development state is basically identical in the material of choosing, each individual plant is carried out labelling, and is measured strain respectively
Height, is expressed as H0, record stem true leaf number, be expressed as N；After mepiquat chloride processes 16～22d, again measure every part of material to be screened
The plant height of each labelling individual plant, is designated as H, calculates the plant height rate of increase of each individual plant, and plant height Growth Rate Calculation method is:
Then the plant height as every part of material to be screened of averaging increases
Rate；Measure between every part of material to be screened each labelling individual plant stem N-1 true leaf and N+2 true leaf, i.e. N, N+1 and N+2
The length of continuous three sections of internodes, calculates the average length of each individual plant these three stem every section of internode of internode, the mark that then will obtain
The average length of internode selected by note individual plant is averaged the restriction stem panel length as every part of material again；
(4) thermo-tolerance evaluation: according to obtaining the plant height rate of increase of every part of material to be screened in step (3) and limiting stem
Panel length, is not less than 50% by plant height rate of increase, and limits stem panel length and be not shorter than the material of 5cm and be divided into high temperature resistant
Type, otherwise belongs to sensitive type.
The field planting detailed process of step (1): cotton seeds to be screened is used nutrition bowl seedling transplanting mode, in
Late April is sowed, or carries out seed sowing, every bunch planting kind 3～4 in early or mid May, and Seedling 1～2 is stayed in 1 every cave of leaf period
Strain, 3 leaf period every cave final singling 1 strains；Every part of detected materials at least plants 50 strains, and density is every 667m2Plantation 2000～3000
Strain, conventionally ensures sufficient rich water supply and normal pest management, does not carry out any chemical regulation seedling stage.
Step (2) preferably carries out mepiquat chloride process in cotton seedling stem leaf length to 10～12 leaf periods.
In step (2), mepiquat chloride is the aqueous solution of concentration 60～120mg/L, preferably 80～the aqueous solution of 100mg/L, often
667m2Using 20～30Kg, preferably 30Kg, Herb uniformly sprays.
Before spray time is 10:00 in the morning or after afternoon 15:00.Spray is avoided rainy day to process, if meeting in 6h after Chu Liing
Rain, fills spray once by the half of initial mepiquat chloride concentration for the treatment of after rain in time again.
The individual plant that in step (3), preferably 10%～20% growth promoter is basically identical from every part of material to be screened.
After preferred mepiquat chloride processes 18～20d in step (3), carry out plant height and limit the measurement of stem panel length.
The screening of the high temperature resisting cotton that the present invention provides and authentication method, have the advantage that and beneficial effect:
(1) simple to operate, method is feasible, and evaluation criterion is directly perceived.The mepiquat chloride used in the present invention be in Cotton Production
Conventional plant growth regulator, has retarding action to cotton nutrient growth, can be conducted extremely by plant leaf and root absorption
Herb, it is possible to decrease the activity of gibberellins in plant body, thus suppress cell elongation, weaken terminal bud growing way, control plant and give birth in length and breadth
Long, make plant shortened internodes.Field test for many years finds, mepiquat chloride sensitivity cotton material all shows flower under high temperature stress
Medicine abnormal development and pollen fertility decline phenomenon, and the mepiquat chloride sensitive property of cotton material reflects its patience journey to high temperature
Degree.The present invention is namely based on this and finds and the screening that proposes and authentication method, in the Cotton Gossypii fast-growth stage of development, passes through plant height
Rate of increase and limit stem panel length evaluate the material sensitivity to mepiquat chloride, thus indirectly but screen intuitively and identify this
The thermo-tolerance degree of material.Those of ordinary skill in the art is grasped easily by the present invention, simple to operate, practical.
(2) need not chamber facility and provide High Temperature Stress, cost-effective, the selection result is accurately and reliably.The method need not
Chamber facility provide high temperature stress condition, it is not required that can not expect occur land for growing field crops natural high-temperature environment, at mepiquat chloride
Cotton material to be screened just can be divided into high temperature resistant and sensitive two types by reason clearly.Result of the test table for many years
Bright, the exotic material that filters out during knot bell of blooming under seasonal High Temperature Stress and artificial hot conditions (35 DEG C～40 DEG C
High temperature continuous processing 7～10d) pollen fertility normal, flower pesticide is full, and pollen amount is big, still shows the feature that knot bell is strong,
Being accredited as the ratio of the most empty flat flower pesticide of material of sensitive type more than 20%, fruit abscission rate is serious, thus screening knot
Fruit is consistent with practical manifestation, reliable results.
(3) Rapid identification in early days, can the breeding utilization of accelerated material.The pollen development of Cotton Gossypii is the quickest to high-temperature response
Sense, but it is flat to need the seasonal High Temperature Stress meeting with the long period just to show flower pesticide sky during the knot bell of blooming in Production of Large Fields
With the performance of being injured of fruit abscission rate, thus late growth stage to be arrived could preferably distinguish the material patience degree to high temperature.This
Bright at Developmental of Cotton in early days (the flower bud phase is to initial bloom stage), thermo-tolerance is carried out Fast Evaluation, can carry out in field fast on a large scale
The screening of speed and appraisal, and typically have only to primary screening, can test without multiple years, thus reflect for material
The breeding plans such as the hybridization transformation in settled year, character polymerization have striven for the time, are conducive to accelerating the breeding utilization of exotic material,
Shorten the breeding of new variety cycle.
The method that the present invention provides is in addition to effectively screening and identify the thermo-tolerance of cotton material, it is also possible to be used for
Evaluate the cotton material sensitivity to plant growth regulator mepiquat chloride, carry for the safe handling of mepiquat chloride in Cotton Gossypii Production of Large Fields
For directive function.
The refractory wool floral material that the present invention filters out still has stronger during the high temperature that 7 to August, seasonality took place frequently
Knot bell, be conducive to the formation of this stage high-quality bell and be concentrated into bell, improving output of cotton and quality.If at late growth stage
Coordinate other chemical regulation measures, be also beneficial to Cotton Gossypii and concentrate blow-of-cottons, reduce artificial receipts and spend number of times and suitable mechanical to receive flower, fall
Low production cost.
Therefore, the method that the present invention provides has good practicality and application prospect.
Detailed description of the invention
It is further elucidated with below by the detailed description of detailed description of the invention, but is not limitation of the present invention, only
Only illustrate.
Embodiment 1
Within 2012, weeding proving ground, garden in the school in Agricultural University Of Hunan uses the present invention to 14 parts of cotton material (title materials
Be shown in Table 1) thermo-tolerance screened and identified.Using nutrition bowl seedling transplanting mode, April 21 was sowed, and May 18 moved
Plant, every 667m2Planting 3000 strains, 100 strains planted by every part of material, and according to general planting management by methods after transplanting, seedling stage is not carried out
Any chemical regulation.To prepare concentration on June 24 is that (mepiquat chloride is that Earthquake of Anyang station in Henan city well-to-do level pesticide is limited to 80mg/L mepiquat chloride
Responsible company produce 97% powder, according to explanation with tap water dissolve preparation) 30Kg solution aerosol apparatus at 9:00 in the morning
Rising and uniformly spray each detected materials, Herb sprays.The most each material all has been enter into containing the flower bud phase, and the open and flat true leaf of stem is all on 10 left sides
Right.From every part of material, growth selection grows basically identical individual plant 10 strain, and each individual plant is carried out labelling, and measured plant height respectively,
Record stem true leaf number.July 13 (after mepiquat chloride processes 19d) again determines strain and measures the strain of each labelling individual plant of every part of material
Height, calculates plant height rate of increase, the plant height rate of increase as every part of material of averaging respectively, and the plant height of 14 parts of materials of statistics increases
Rate；Measure the stem true leaf number-1 when each labelling individual plant of every part of material processes between stem true leaf number+2 continuous three sections simultaneously
The length of internode, calculates the average length of every section of internode, then the average length of internode selected by the labelling individual plant of acquisition is taken again
Meansigma methods, as the restriction stem panel length of every part of material, is added up 14 parts of materials to be screened and is limited stem panel length (table 1).
According to 14 parts of detected materials mepiquat chloride process after plant height rate of increase and limit stem panel length, HLY11 and
The plant height rate of increase that Soviet Union is cotton No. 16, more than 50%, limits stem panel length and is more than 5cm, be divided into high temperature resistant type, other
12 parts of materials are then divided into sensitive type (table 1).Visible, according to this invention screening and qualification exotic material out
It is consistent with our result of previous publications paper (being shown in Table the document in 1 remarks).
Table 1 cotton material plant height rate of increase, stem joint length and thermo-tolerance type
Remarks: the detected materials in this table is previously reported by paper Zhi Liu (Liu Zhi) with us, You-Lu Yuan,
Shao-Qing Liu,Xiao-Nan Yu and Li-Qun Rao.Screening for High-Temperature
Tolerant Cotton Cultivars by Testing in vitro Pollen Germination,Pollen Tube
Growth and Boll Retention.Journal of Integrative Plant Biology,2006,48(6):
Research material in 706-714 is consistent, is the English name used in this paper in bracket.
Embodiment 2
For accuracy and the using value of the checking present invention, within 2013, close mountain village at Changsha, Hunan Ningxiang County and continue to plant
Plant 14 parts of cotton materials in embodiment 1.Using nutrition bowl seedling transplanting mode, sowed April 13, May 13 transplanted, often
667m2Planting 2500 strains, 100 strains planted by every part of material, according to general planting management by methods after transplanting.On June about 20 each material
Material all enters and contains the flower bud phase.The hot weather more than 35 DEG C in one period has been met with, subsequently from June 30 from 13 to 25 June
The high temperature and drought weather of rare continuous more than 50 days was occurred in that to August 19.By the abiogenous high temperature stress in field, we
The thermo-tolerance of the cotton material utilizing the present invention to screen in embodiment 1 and identify is confirmed.During this period, root
According to the growth of cereal crop seedlings use man-made irrigation with guarantee test field supply water, apply fertilizer normally according to Routine Management method, chemical regulation and
The prevention and control of plant diseases, pest control.Between Cotton Gossypii flowering and boll-setting period, pass twice through hands sth. made by twisting flower pesticide in July 15 and August 10 respectively and judge that flower pesticide sky is flat
Degree and pollen grain quantity, and add up the percentage ratio of the empty flat flower pesticide of every part of material.Investigated single-strain bell-forming number August 20, calculate
Boll per plant rate, the strain of every part of material random searching 10, then add up average Bolls per plant and the bolling rate (table of 14 parts of materials
2).As shown in Table 2, under high temperature stress for a long time, the anther development of HLY11 and cotton No. 16 of Soviet Union is normal, full and flower
Powder amount is big, and flat almost without sky, fruit abscission rate is few, and single-strain bell-forming number and bolling rate are high, show the strongest knot bell, high temperature resistant
Characteristic is obvious；The empty flat rate of the flower pesticide of other 12 parts of materials is all more than 20%, and fruit abscission rate is more serious, and knot bell is poor, to high temperature
Performance sensitivity, the newest excellent cotton 68 and TS18 show male-sterile character, high temperature during do not become bell, the quickest to high temperature
Sense.These results confirm the present invention accuracy to high temperature resisting cotton screening with qualification result.
The empty flat rate of table 2 cotton anthers and boll per plant
| Title material | The empty flat rate (%) of flower pesticide | Single-strain bell-forming number (individual) | Boll per plant rate (%) |
| HLY11 | 0 | 38.6 | 74.3 |
| Cotton No. 4 of nasal mucus | 24.5 | 25.8 | 53.6 |
| JC108 | 22.3 | 26.2 | 51.7 |
| New excellent cotton 68 | 100 | 4.4 | 10.5 |
| Cotton No. 12 of Soviet Union | 42.3 | 14.7 | 24.5 |
| NuCOTN33B | 83.6 | 9.8 | 16.6 |
| Middle cotton 9409 | 36.2 | 17.8 | 38.4 |
| CCRI 41 | 34.7 | 19.2 | 35.8 |
| Zhongmiansuo 12 | 37.1 | 17.0 | 31.6 |
| TS18 | 100 | 3.2 | 9.4 |
| Shandong cotton 584 | 28.2 | 25.4 | 51.2 |
| Cotton No. 16 of Soviet Union | 3.8 | 30.6 | 67.3 |
| HLY15 | 86.4 | 11.2 | 15.2 |
| Cotton No. 3 of nasal mucus | 23.8 | 27.2 | 57.6 |
Embodiment 3
(title material is shown in 10 cotton varieties within 2014, to use the present invention in village of the Temple of the Dragon King, Chun Hua town, ChangSha, Hunan Province county
Table 3) thermo-tolerance screened and identified.Seed sowing was carried out May 12, every part of live 100 caves of material to be identified,
Every bunch planting kind 3～4, Seedling 1～2 strain, 3 leaf period every cave final singling 1 strains are stayed in 1 every cave of leaf period.Planting density is every 667m2
Plant 2000 strains, conventionally ensure sufficient rich water supply and normal pest management, do not carry out any chemistry seedling stage and adjust
Control.The 30Kg that concentration is 100mg/L mepiquat chloride (mepiquat chloride source and compound method are same as in Example 1) will be prepared on July 6
Uniformly spraying each detected materials after solution aerosol apparatus 15:00 in the afternoon, Herb sprays.The most each material all has been enter into containing flower bud
Phase, the open and flat true leaf of stem is all at about 10.From every part of material, growth selection grows basically identical individual plant 15 strain, each individual plant
Carry out labelling, and measure plant height respectively, record stem true leaf number.Again determine strain July 25 (after mepiquat chloride processes 18d) and measure every
The plant height of part each labelling individual plant of material, respectively calculating plant height rate of increase, the plant height rate of increase as every part of material of averaging,
The plant height rate of increase of 10 parts of materials of statistics；Measure the stem true leaf number-1 when each labelling individual plant of every part of material processes to master simultaneously
Between stem true leaf number+2, the length of continuous three sections of internodes, calculates the average length of every section of internode, selected by the labelling individual plant that will obtain
The average length of internode is averaged the restriction stem panel length as every part of material again, and then 10 parts of kinds of statistics limit main
Stem panel length.According to 10 parts of cotton varieties to be measured plant height rate of increase after mepiquat chloride processes and restriction stem panel length,
Wherein cotton No. 1 of Hunan agricultural university, miscellaneous cotton No. 23 of Hunan, cotton No. 11 of Hunan agriculture miscellaneous cotton 68 and Ya Hua plant height rate of increase more than 50%, limit
Stem panel length is more than 5cm, and these 4 kinds are divided into high temperature resistant type, and other 6 kinds are then divided into sensitive class
Type (table 3).
From continuous 9d on July 29 to August 6, the high temperature of more than 35 DEG C occurs in this proving ground, adjusted August 20 subsequently
Single-strain bell-forming number and the fruit abscission rate rate of 10 kinds to be measured, the strain of every part of material random searching 20 are looked into.Table 4 result shows, mirror
Cotton No. 1 of the Hunan agricultural university made, miscellaneous cotton No. 23 of Hunan, the cotton buds and bolls of cotton No. 11 4 the refractory wool flower varieties of Hunan agriculture miscellaneous cotton 68 and Ya Hua take off
The rate that falls is low, and upper, middle and lower Fruit branch knot bell is more uniform, and single-strain bell-forming number is many, and knot bell is relatively concentrated；And the flower bud of other 6 sensitive kinds
Bell expulsion rate is the highest, is coerced by natural high-temperature and affects and cause middle part Fruit branch Boll probability low, and sensitive is obvious, thus enters one
Step demonstrates present invention screening and the reliability of qualification result.
Table 3 cotton variety plant height rate of increase, stem joint length and thermo-tolerance type
Table 4 cotton variety fruit abscission rate rate and single-strain bell-forming number
| Title material | Fruit abscission rate rate (%) | Single-strain bell-forming number (individual) |
| CCRI 70 | 68.4 | 27.8 |
| Rich cotton No. 6 of Hunan | 63.5 | 23.1 |
| CCRI 60 | 71.6 | 20.3 |
| Cotton No. 1 of Hunan agricultural university | 30.5 | 44.2 |
| Miscellaneous cotton No. 23 of Hunan | 34.7 | 40.6 |
| Ji excellent 861 | 72.8 | 24.4 |
| Shandong cotton grinds No. 40 | 65.4 | 27.5 |
| Hunan agriculture miscellaneous cotton 68 | 36.6 | 39.8 |
| Shandong cotton grinds No. 37 | 73.5 | 25.7 |
| Cotton No. 11 of sub-China | 40.2 | 36.3 |
Claims (7)
1. the screening of a high temperature resisting cotton and authentication method, it is characterised in that comprise the following steps:
(1) field planting: use nutrition bowl seedling transplanting or seed sowing mode field planting material to be screened；
(2) mepiquat chloride processes: carry out mepiquat chloride process in cotton seedling stem leaf length to 8～12 leaf periods；Mepiquat chloride is dense
The aqueous solution of degree 60～120mg/L, every 667m2Using 20～30Kg, Herb uniformly sprays；Before spray time is 10:00 in the morning
Or after afternoon 15:00；Avoiding rainy day spray to process, if meeting rain after Chu Liing in 6h, processing dense by initial mepiquat chloride in time after rain
The half of degree fills spray once again；
(3) determine strain measure plant height and limit the length of stem internode: on the same day carrying out mepiquat chloride process, to be screened from every part
Selecting the individual plant that at least 10% growth and development state is basically identical in material, each individual plant carries out labelling, and measures plant height, table respectively
It is shown as H0, record stem true leaf number, be expressed as N；Mepiquat chloride processes after 16～22d, again measure every part of material to be screened each
The plant height of labelling individual plant, is designated as H, calculates the plant height rate of increase of each individual plant, and plant height Growth Rate Calculation method is:
Then average the plant height rate of increase as every part of material to be screened；Measure
Between every part of material to be screened each labelling individual plant stem N-1 true leaf and N+2 true leaf, i.e. continuous three sections of N, N+1 and N+2
The length of internode, calculates the average length of each individual plant these three stem every section of internode of internode, the labelling individual plant institute that then will obtain
The average length selecting internode is averaged the restriction stem panel length as every part of material again；
(4) thermo-tolerance evaluation: according to obtaining the plant height rate of increase of every part of material to be screened in step (3) and limiting stem internode
Length, is not less than 50% by plant height rate of increase, and limits stem panel length and be not shorter than the material of 5cm and be divided into high temperature resistant class
Type, otherwise belongs to sensitive type.
The screening of high temperature resisting cotton the most according to claim 1 and authentication method, it is characterised in that
The field planting detailed process of step (1): cotton seeds to be screened is used nutrition bowl seedling transplanting mode, April
The last ten-days period are sowed, or carry out seed sowing, every bunch planting kind 3～4 in early or mid May, and Seedling 1～2 strain is stayed in 1 every cave of leaf period, and 3
Every cave final singling 1 strain of sheet leaf period；Density is every 667m2Plantation 2000～3000 strains, conventionally ensure that sufficient rich water supplies
Should be with normal pest management, seedling stage does not carry out any chemical regulation.
The screening of high temperature resisting cotton the most according to claim 2 and authentication method, it is characterised in that every part of detected materials is extremely
Plant 50 strains less.
The screening of high temperature resisting cotton the most according to claim 1 and authentication method, it is characterised in that step (2) is in Cotton Gossypii
Seedling stem leaf length carries out mepiquat chloride process to 10～12 leaf periods.
5. according to screening and the authentication method of the high temperature resisting cotton described in claim 1 or 4, it is characterised in that contracting in step (2)
Joint amine is the aqueous solution of concentration 80～100mg/L, every 667m2Use 30Kg.
The screening of high temperature resisting cotton the most according to claim 1 and authentication method, it is characterised in that from often in step (3)
The material that part is to be screened selects the individual plant that 10%～20% growth promoter is basically identical.
The screening of high temperature resisting cotton the most according to claim 1 and authentication method, it is characterised in that casing coupling in step (3)
After amine processes 18～20d, carry out plant height and limit the measurement of stem panel length.
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| CN102577802A (en) * | 2012-02-22 | 2012-07-18 | 中国农业科学院棉花研究所 | Method for rapid identification of heat-resistance seedling state of cotton |
| CN103828580B (en) * | 2014-02-27 | 2015-09-23 | 中棉种业科技股份有限公司 | The resistant to elevated temperatures screening technique of a kind of field test cotton |
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