CN102433215A - Method of extracting grease from fungi or algae through physical wall-breaking - Google Patents
Method of extracting grease from fungi or algae through physical wall-breaking Download PDFInfo
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- CN102433215A CN102433215A CN2011102835257A CN201110283525A CN102433215A CN 102433215 A CN102433215 A CN 102433215A CN 2011102835257 A CN2011102835257 A CN 2011102835257A CN 201110283525 A CN201110283525 A CN 201110283525A CN 102433215 A CN102433215 A CN 102433215A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/06—Production of fats or fatty oils from raw materials by pressing
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/16—Refining fats or fatty oils by mechanical means
Abstract
The invention discloses a method of extracting grease from fungi or algae cells rich in grease through physical wall-breaking. The method mainly comprises the following steps: separating fungi or algae fermentation broth obtained after fermentation by using a separating system, collecting cells, controlling cell mud, carrying out physical wall-breaking on the cell mud by using a colloid mill or a high pressure homogenizer, carrying out secondary ball milling wall breaking and emulsion breaking by using a ball mill, and separating feed liquid with a three-phase seperator so as to obtain grease rich in polyunsaturated fatty acid. According to the invention, a physical wall breaking and extraction method is utilized, so a simple process and high wall breaking efficiency of cells are achieved; active components in algae and fungi cells can be effectively protected because of low temperature and anti-oxidizing treatment; an obtained product is green, nontoxic and residue-free; the method can be used for extracting DHA grease, ARA grease, EPA grease, GLA and ALA grease, etc., and is suitable for industrial production.
Description
Technical field
The present invention relates to a kind of from fungi or algae the method for extracting effective components, particularly relate to a kind of from fungi or algae physical wall breaking extract greasy method.
Background technology
Pufas (polyunsaturated fatty acid; PUFA) generally be meant and contain two or more pairs key; The straight chain fatty acid of carbon chain lengths 16-22; Position difference according to two keys place carbon atom is divided into serial unsaturated fatty acids of ω-3 and the serial unsaturated fatty acids of ω-6 again, and wherein linolenic acid (GLA), timnodonic acid (EPA), docosahexenoic acid (DHA) belong to ω-3 series, and linolic acid, arachidonic acid (ARA) belong to ω-6 series.It is active that PUFA has many important physical, and it can regulate the homergy of interior blood fat of human body and lipoprotein, reduces cholesterol level in blood viscosity and the blood; The protection vessel wall; Prevent the formation of cerebral thrombosis, have the prevention myocardial infarction, hypertension, cardiopathic function; Thereby it can be converted to the effect that prostanoid plays immunne response in human body simultaneously, and aspects such as enhance immunity are all had positive effect; Some composition in the unsaturated fatty acids still is the indispensable nutritive substance of brain and nervous system development in addition; Can promote the neural growth of fetus brain like the DHA in the pufas; Significantly improve the infant intelligent development level; Simultaneously, DHA delays the brain aging and plays an important role keeping the brain function.
Human body self can not synthesize polyunsaturated fatty acid, but it is again one of indispensable important substance of human body, therefore can only satisfy the human body needs through absorbing from the external world.The main source of traditional unsaturated fatty acids is a fish oil, but fish oil itself has many shortcomings, as: unpleasant fishy smell, mouthfeel be not good, contain SUV and the erucic acid that is bound to arouse fear etc., greatly influences the quality of product; And unsaturated fatty acid content receives influences such as season, area, kind and fish stock restriction easily in the fish oil; In addition, owing to receive the influence of environmental pollution, the fish of certain areas possibly contain heavy metal and other pollutents of high level, and this can extra increase extraction cost.
Discover, contain a large amount of unsaturated fatty acidss in many fungies and the alga cells.From fungi or algae, extract unsaturated fatty acids and can overcome existing the problems referred to above of extraction unsaturated fatty acids from fish oil; And fungi and algae can carry out mass artificial and cultivate; Output is high; Cycle is short, therefore utilizes fungi and algae to extract unsaturated fatty acids as the alternate resources of fish oil and has far reaching significance.
Because fungi and algae self cell walls, the problem of from fungi or alga cells, extracting the maximum of unsaturated fatty acids is a cell wall breaking.The domestic method of from fungi or alga cells, extracting unsaturated fatty acids is: organic solvent method, acid heat method, enzyme process etc.Organic solvent method has advantage simple to operate, but time-consuming, and extraction efficiency is low, has problems such as product residue organic solvent and production safety hidden danger simultaneously.Adopt the acid heat method can destroy the cell walls of fungi or algae fast and effectively, but reaction conditions is violent, destroys desired product composition in the cell easily, and have problems such as sour residual.Adopt enzymatic shell-broken, though reaction conditions is gentle, equipment is simple; But because the difference of cell walls moity between different microorganisms, different microorganisms iuntercellular shell-broken effect significant difference is simultaneously because enzymatic hydrolysis condition is harsh than additive method; Therefore the enzymolysis cost is higher, is not suitable for suitability for industrialized production.
Summary of the invention
The present invention aims to provide a kind of the auxiliary of any organic solvent that need not, and the method for employing physics is to fungi or alga cells carries out broken wall and extracts grease in the born of the same parents, is suitable for scale operation cheaply.
For achieving the above object, technical scheme proposed by the invention is: a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: comprise the steps:
Step 1: fungi after the fermentation ends or algae fermented liquid are passed through the separation system collecting cell, keep fungi or frustule mud water cut at 70-90%;
Step 2: control cell mud temperature is at 15-40 ℃;
Step 3: use acid or alkali to regulate cell mud PH and be 0-5 or 8-14;
Step 4: the inhibitor that in cell mud, adds cell shale amount 1-3%;
Step 5: the cell dredge pump is gone into colloidal mill or high pressure homogenizer carries out broken wall, and controlled temperature is at 30-95 ℃;
Step 6: the cell mud that colloidal mill or high pressure homogenizer come out carries out secondary broken wall and breakdown of emulsion through ball mill, and controlled temperature is at 30-95 ℃;
Step 7: the cell mud behind the broken wall is warming up to 30-95 ℃ under the agitation condition of 20-150rpm, and keeps 0.5-5 hour;
Step 8: the bottom adds hot water through gas distribution pipe by weight cell Ni ﹕ water=1 ﹕ 2.5-6.5 from cell mud, and controlled temperature 30-95 ℃, stir 50-80min at 250-350rpm, feed liquid is separated obtaining grease through three-phase separator.
Further, described in the step 4 inhibitor be vitamin E, natural Rosmarinus officinalis, a kind of in the anti-bad courageous and upright sour cetylate or their mixture.
Further, the WP of the high pressure homogenizer described in the step 6 is 120-130Mpa.
Further, the working speed of colloidal mill is below the 14000rpm in the step 6.
Further, described acid is hydrochloric acid, sulfuric acid, Hydrocerol A or lactic acid.
Further, described alkali is sodium hydroxide.
Further, described ball mill is sand mill or ball mill etc., and its WP is 20-30Mpa.
Further, described algae is for splitting kettle algae or dino flagellate, and grease is the DHA grease.
Further, described fungi is a Mortierella alpina, and grease is the ARA grease.
Further, described fungi is a Mortierella isabellina, and grease is the EPA grease.
Further, described fungi is little Ke Yinhan, and grease is GLA and ALA grease.
The present invention has overcome and must carry out drying traditionally and process powder and just pulverize, and the technological barrier that is difficult to the hard cell walls of broken fungi or algae, has realized low-cost and the cell walls of high efficiency broken algae and fungi.Its concrete characteristic is following: (1) is for avoiding fungi and alga cells mud under high velocity impact or high-pressure squeezing condition; Be easy to generate heat; And the biological activity of destruction intracellular product; The means of the present invention's employing cooling in advance are reduced to 15-40 ℃ with the temperature of fungi and alga cells mud; Adjust simultaneously storage that pH more helps feed liquid controlled effectively fungi and alga cells mud particularly in the born of the same parents polyunsaturated fatty acid grease be exposed in the high temperature, the effective constituent of having avoided Yin Gaowen to cause is decomposed or inactivation, adjusts pH simultaneously and more helps the storage of feed liquid and improve sporoderm-broken rate; (2) added protective material in advance; In high pressure homogenizer or colloidal mill broken wall process; Protective material can be uniformly and effectively with grease behind the broken wall or particle embedding; Form effectively protection barrier, thus reduce pufas in the born of the same parents greatly with the contacting of air, reduce the possibility of the oxidized decomposition of polyunsaturated fatty acid grease.(3) adopt the process for extracting of physics, overcome the residual and hidden peril of explosion that organic solvent traditionally brings, a kind of product of safe green nontoxic residue-free is provided; (4) the cell mud that adopts colloidal mill or high pressure homogenizer to come out carries out secondary broken wall and breakdown of emulsion through ball mill, has improved productive rate.
Embodiment
Below in conjunction with specific embodiment, the present invention is further specified.
Embodiment 1
1) the kettle algae fermented liquid that splits after the fermentation ends is passed through separation system separated and collected frustule, keep algae mud water cut at 70%-90%; Algae mud is collected in the storage tank that has spiral coil cooling tube and a stirring,
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of algae mud to reduce to 15-40 ℃, close cooling water intakeoutfall;
3) under stirring state, utilize acid for adjusting pH, make algae mud pH between 0.0-5.0;
4) under stirring state, add 3% antioxidant blends (vitamin E 1%, natural Rosmarinus officinalis 0.5%, anti-bad courageous and upright sour cetylate 1.5%) according to algae mud weight ratio, mix;
5) the algae dredge pump is gone in the paulownia body of high pressure homogenizer, the WP of regulating high pressure homogenizer is 120-130Mpa, and the temperature that keeps high pressure homogenizer is carried out high-pressure homogeneous broken wall at 30-95 ℃ with algae mud, and the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 30Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the kettle algae algae mud that splits behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight Zao Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from algae mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min, feed liquid is separated obtaining the DHA grease through three-phase separator, extraction yield is 93%.
Embodiment 2
1) dino flagellate fermentation liquor after the fermentation ends is utilized separation system separated and collected dino flagellate cell, keep algae mud water cut at 70%-90%; Algae mud is collected in the storage tank that has spiral coil cooling tube and a stirring;
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of algae mud to reduce to 20 ℃, close cooling water intakeoutfall;
3) under the high-speed stirring situation, utilize acid for adjusting pH, make between the algae mud pH 0.0-5.0;
4) under the high-speed stirring situation, add 1% antioxidant blends (vitamin E 0.3%, natural Rosmarinus officinalis 0.5%, anti-bad courageous and upright sour cetylate 0.2%) according to algae mud weight ratio, mix;
5) with the algae dredge pump go into colloidal mill in, the working speed of regulating colloidal mill is below the 14000rpm, controlled temperature carries out broken wall at 30-95 ℃ with algae mud, the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 20Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the dino flagellate algae mud behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight Zao Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from algae mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min, feed liquid is separated obtaining the DHA grease through three-phase separator, extraction yield is 88%.
Embodiment 3
1) the Mortierella alpina fermented liquid after the fermentation ends is passed through separation system separated and collected fungal cell, keep cell mud water cut at 70%-90%; Cell mud is collected in the storage tank that has spiral coil cooling tube and a stirring,
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of cell mud to reduce to 15-40 ℃, close cooling water intakeoutfall;
3) under stirring state, utilize acid for adjusting pH, make between the cell mud pH 0.0-5.0;
4) under stirring state, add 2% antioxidant blends (vitamin E 0.5%, natural Rosmarinus officinalis 0.5%, anti-bad courageous and upright sour cetylate 1%) according to cell mud weight ratio, mix;
5) the cell dredge pump is gone in the paulownia body of high pressure homogenizer; The WP of regulating high pressure homogenizer is 120-130Mpa; The temperature that keeps high pressure homogenizer is carried out high-pressure homogeneous broken wall at 30-95 ℃ with cell mud, and the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 25Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the cell mud behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight cell Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from cell mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min; Feed liquid is obtained pufas ARA grease through the three-phase separator separation, and extraction yield is 83%.
Embodiment 4
1) the Mortierella isabellina fermented liquid after the fermentation ends is passed through separation system separated and collected fungal cell, keep cell mud water cut at 70%-90%; Cell mud is collected in the storage tank that has spiral coil cooling tube and a stirring,
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of cell mud to reduce to 15-40 ℃, close cooling water intakeoutfall;
3) under stirring state, utilize acid to carry out the allotment of pH, make between the cell mud pH 0.0-5.0;
4) under stirring state, add 1% antioxidant vitamin E according to cell mud weight ratio, mix;
5) with the cell dredge pump go into colloidal mill in, the working speed of regulating colloidal mill is below the 14000rpm, controlled temperature carries out high-pressure homogeneous broken wall at 30-95 ℃ with cell mud, the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 30Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the cell mud behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight cell Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from cell mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min; Feed liquid is obtained pufas EPA grease through the three-phase separator separation, and extraction yield is 85%.
Embodiment 5
1) the little Ke Yinhan fermented liquid after the fermentation ends is passed through separation system separated and collected fungal cell, keep cell mud water cut at 70%-90%; Cell mud is collected in the storage tank that has spiral coil cooling tube and a stirring,
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of cell mud to reduce to 15-40 ℃, close cooling water intakeoutfall;
3) under stirring state, utilize acid for adjusting pH, make between the cell mud pH 0.0-5.0;
4) under stirring state, add the natural Rosmarinus officinalis of 2% inhibitor according to cell mud weight ratio, mix;
5) the cell dredge pump is gone in the paulownia body of high pressure homogenizer; The WP of regulating high pressure homogenizer is 120-130Mpa; The temperature that keeps high pressure homogenizer is carried out high-pressure homogeneous broken wall at 30-95 ℃ with cell mud, and the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 20Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the cell mud behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight cell Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from cell mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min; Feed liquid is obtained pufas GLA and ALA grease through the three-phase separator separation, and extraction yield is 82%.
Embodiment 6
1) the kettle algae fermented liquid that splits after the fermentation ends is passed through separation system separated and collected frustule, keep algae mud water cut at 70%-90%; Algae mud is collected in the storage tank that has spiral coil cooling tube and a stirring,
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of algae mud to reduce to 15-40 ℃, close cooling water intakeoutfall;
3) under stirring state, utilize alkali to regulate pH, make between the algae mud pH 8.0-14.0;
4) under stirring state, add the anti-bad courageous and upright sour cetylate of 3% inhibitor according to algae mud weight ratio, mix;
5) the algae dredge pump is gone in the paulownia body of high pressure homogenizer, the WP of regulating high pressure homogenizer is 120-130Mpa, and the temperature that keeps high pressure homogenizer is carried out high-pressure homogeneous broken wall at 30-95 ℃ with algae mud, and the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 25Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the kettle algae algae mud that splits behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight Zao Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from algae mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min, feed liquid is separated obtaining the DHA grease through three-phase separator, extraction yield is 94%.
Embodiment 7
1) dino flagellate fermentation liquor after the fermentation ends is utilized separation system separated and collected dino flagellate cell, keep algae mud water cut at 70%-90%; Algae mud is collected in the storage tank that has spiral coil cooling tube and a stirring;
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of algae mud to reduce to 20 ℃, close cooling water intakeoutfall;
3) under the high-speed stirring situation, utilize alkali to regulate pH, make between the algae mud pH 8.0-14.0;
4) under the high-speed stirring situation, add 2% antioxidant blends (vitamin E 1.5%, natural Rosmarinus officinalis 0.5%) according to algae mud weight ratio, mix;
5) with in the algae mud colloidal mill, the working speed of regulating colloidal mill is below the 14000rpm, and controlled temperature carries out high-pressure homogeneous broken wall at 30-95 ℃ with algae mud, and the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 30Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the dino flagellate algae mud behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight Zao Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from algae mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min, feed liquid is separated obtaining the DHA grease through three-phase separator, extraction yield is 91%.
Embodiment 8
1) the Mortierella alpina fermented liquid after the fermentation ends is passed through separation system separated and collected fungal cell, keep cell mud water cut at 70%-90%; Cell mud is collected in the storage tank that has spiral coil cooling tube and a stirring,
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of cell mud to reduce to 15-40 ℃, close cooling water intakeoutfall;
3) under stirring state, utilize alkali to regulate pH, make between the cell mud pH 8.0-14.0;
4) under stirring state, add 2.5% antioxidant blends (vitamin E 1%, anti-bad courageous and upright sour cetylate 1.5%) according to cell mud weight ratio, mix;
5) the cell dredge pump is gone in the paulownia body of high pressure homogenizer; The WP of regulating high pressure homogenizer is 120-130Mpa; The temperature that keeps high pressure homogenizer is carried out high-pressure homogeneous broken wall at 30-95 ℃ with cell mud, and the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 30Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the cell mud behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight cell Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from cell mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min; Feed liquid is obtained pufas ARA grease through the three-phase separator separation, and extraction yield is 85%.
Embodiment 9
1) the Mortierella isabellina fermented liquid after the fermentation ends is passed through separation system separated and collected fungal cell, keep cell mud water cut at 70%-90%; Cell mud is collected in the storage tank that has spiral coil cooling tube and a stirring,
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of cell mud to reduce to 15-40 ℃, close cooling water intakeoutfall;
3) under stirring state, utilize alkali to regulate pH, make between the cell mud pH 8.0-14.0;
4) under stirring state, add 1.5% antioxidant blends (vitamin E 1%, natural Rosmarinus officinalis 0.5%) according to cell mud weight ratio and mix;
5) the cell dredge pump is gone in the paulownia body of high pressure homogenizer; The WP of regulating high pressure homogenizer is 120-130Mpa; The temperature that keeps high pressure homogenizer is carried out high-pressure homogeneous broken wall at 30-95 ℃ with cell mud, and the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 25Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the cell mud behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight cell Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from cell mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min; Feed liquid is obtained pufas EPA grease through the three-phase separator separation, and extraction yield is 85%.
Embodiment 10
1) the little Ke Yinhan fermented liquid after the fermentation ends is passed through separation system separated and collected fungal cell, keep cell mud water cut at 70%-90%; Cell mud is collected in the storage tank that has spiral coil cooling tube and a stirring,
2) be that 10 ℃ of water coolants cool off through in the coil pipe of tank body, feeding temperature, wait the temperature of cell mud to reduce to 15-40 ℃, close cooling water intakeoutfall;
3) under stirring state, utilize alkali to regulate pH, make between the cell mud pH 8.0-14.0;
4) under stirring state, add 3% antioxidant blends (vitamin E 1%, natural Rosmarinus officinalis 0.5%, anti-bad courageous and upright sour cetylate 1.5%) according to cell mud weight ratio, mix;
5) the cell dredge pump is gone in the paulownia body of high pressure homogenizer; The WP of regulating high pressure homogenizer is 120-130Mpa; The temperature that keeps high pressure homogenizer is carried out high-pressure homogeneous broken wall at 30-95 ℃ with cell mud, and the feed liquid behind the broken wall is directly squeezed in the storage tank of ball mill;
6) pressure of adjusting ball mill is 20Mpa, and controlled temperature carries out secondary broken wall and breakdown of emulsion at 30-95 ℃, and the feed liquid behind the secondary broken wall is packed into and had in the storage tank of gas distribution pipeline and coil pipe;
7) the cell mud behind the broken wall is warmed up to 30-95 ℃, under 20-150rpm stirs, heats and kept 0.5-5 hour;
8) ratio by weight cell Ni ﹕ water=1 ﹕ 2.5-6.5 adds hot water from cell mud bottom through gas distribution pipe; And control is expected warm 30-95 ℃; Add an amount of edible salt and under 250-350rpm stirs, keep 50-80min; Feed liquid is obtained pufas GLA and ALA grease through the three-phase separator separation, and extraction yield is 87%.
Although specifically show and introduced the present invention in conjunction with preferred embodiment; But the those skilled in the art should be understood that; In the spirit and scope of the present invention that do not break away from appended claims and limited; In form with on the details the present invention is made various variations, be protection scope of the present invention.
Claims (10)
- One kind from fungi or algae physical wall breaking extract greasy method, it is characterized in that: comprise the steps:Step 1: fungi after the fermentation ends or algae fermented liquid are passed through the separation system collecting cell, keep fungi or frustule mud water cut at 70-90%;Step 2: control cell mud temperature is at 15-40 ℃;Step 3: use acid or alkali to regulate cell mud PH and be 0-5 or 8-14;Step 4: the inhibitor that in cell mud, adds cell shale amount 1-3%;Step 5: the cell dredge pump is gone into colloidal mill or high pressure homogenizer carries out broken wall, and controlled temperature is at 30-95 ℃;Step 6: the cell mud that colloidal mill or high pressure homogenizer come out carries out secondary broken wall and breakdown of emulsion through ball mill, and controlled temperature is at 30-95 ℃;Step 7: the cell mud behind the broken wall is warming up to 30-95 ℃ under the agitation condition of 20-150rpm, and keeps 0.5-5 hour;Step 8: the bottom adds hot water through gas distribution pipe by weight cell Ni ﹕ water=1 ﹕ 2.5-6.5 from cell mud, and controlled temperature 30-95 ℃, stir 50-80min at 250-350rpm, feed liquid is separated obtaining grease through three-phase separator.
- 2. according to claim 1 a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: described in the step 4 inhibitor be vitamin E, natural Rosmarinus officinalis, a kind of in the anti-bad courageous and upright sour cetylate or their mixture.
- 3. according to claim 1 a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: the WP of the high pressure homogenizer described in the step 6 is 120-130Mpa.
- 4. according to claim 1 a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: the working speed of colloidal mill is below the 14000rpm in the step 6.
- 5. according to claim 1 a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: described acid is hydrochloric acid, sulfuric acid, Hydrocerol A or lactic acid, described alkali is sodium hydroxide.
- 6. according to claim 1 a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: described ball mill is sand mill or ball mill, its WP is 20-30Mpa.
- According to the arbitrary claim of claim 1-6 described a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: described algae is for splitting kettle algae or dino flagellate, and grease is the DHA grease.
- According to the arbitrary claim of claim 1-6 described a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: described fungi is a Mortierella alpina, grease is the ARA grease.
- According to the arbitrary claim of claim 1-6 described a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: described fungi is a Mortierella isabellina, grease is the EPA grease.
- According to the arbitrary claim of claim 1-6 described a kind of from fungi or algae physical wall breaking extract greasy method, it is characterized in that: described fungi is little Ke Yinhan, grease is GLA and ALA grease.
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| CN2011102835257A CN102433215A (en) | 2011-09-22 | 2011-09-22 | Method of extracting grease from fungi or algae through physical wall-breaking |
| PCT/CN2011/001799 WO2013040732A1 (en) | 2011-09-22 | 2011-10-27 | Method for extracting grease from fungi or alga through physical wall-breaking |
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| CN2011102835257A CN102433215A (en) | 2011-09-22 | 2011-09-22 | Method of extracting grease from fungi or algae through physical wall-breaking |
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| CN102433215A true CN102433215A (en) | 2012-05-02 |
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| WO (1) | WO2013040732A1 (en) |
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