CN1570077A - Grifola frondosa mycelia moisture method ultramicro wall break method - Google Patents
Grifola frondosa mycelia moisture method ultramicro wall break method Download PDFInfo
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Abstract
A wet method ultramicro wall breaking method of ash tree flower myceliumis is disclosed. It includes the steps: 1. obtaining and pretreating of the ash tree flower fungus fermentation liquid 2. first level wall breaking by wet method: with the gap between the colloid mill stator and the rotor regulated to 0.5 to 1 mm, the colloid mil flow rate regulated to 0.1 to 1 ton/hours, the mycelium with granularity of 0.4 to 0.8 mm after broken the wall accounts for 70%. 3. secondary level ultromacro wall breaking by wet method, the pressure of the high pressure homogenizer being 60-100Mpa, the flow rate of the high pressure homogenizer being 0.1 to 1 ton/hours, the treated material with granularity of 40 to 100 nanometers accounts for 80%. It combines the colloid mill with highly pressure homogenizer, adopts two stage wet method ultromicro wall breaking technology to treat the ash tree mycelium in fermenting liquor to realize mycelium function factor full rate leaching. The treated material provided by the invention can not only be a material for extracting the effective components of ash tree flower polysaccharide but also can be directly processed to liquid or solid further processed product.
Description
Technical field
The invention belongs to the fungi fermentation industrial circle, particularly relate to the maitake mushroom mycelia wet method ultra micro wall-breaking method that a kind of tank fermentation method is cultivated.
Background technology
Grifola frondosa (Polyporus frondosus) is under the jurisdiction of Mycophyta, Basidiomycotina, Aphyllophorales, polyporaceae, Polyporus.Grifola frondosa is famous edible, medicinal fungi, and is nutritious, tasty, is the macro fungi of " but edible, can mend medicine, the whole body is precious ".Grifola frondosa is flat, flavor is sweet, can be used for treating diseases such as dysuria, oedema, beriberi, hepatic ascites, diabetes, hypertension and obesity.The functional component grifolan that contains in the Grifola frondosa has tangible antitumous effect.At present, domestic and international research all concentrates on the extraction of Grifola frondosa fungi submerged fermentation method, Grifola frondosa fungus polysaccharide, the molecular structure of Grifola frondosa fungus polysaccharide and the aspects such as pharmacological effect of polysaccharide mostly.
Through the maitake mushroom mycelia that tank fermentation method is produced, reach cell walls in the cell and contain a large amount of function nutrition compositions, because the existence of cell walls has hindered effective release of function nutrition composition, also influenced the absorption of human body to its function nutrition composition.The present invention adopts wet method ultra micro broken wall treatment maitake mushroom mycelia, has realized the full price stripping and efficient utilization of function nutrition composition in the mycelium, has set up to simultaneity factor wet method ultra micro wall-breaking technology, has further expanded the range of application of maitake mushroom mycelia.
Summary of the invention
The purpose of this invention is to provide a kind of maitake mushroom mycelia wet method ultra micro wall-breaking method.
Technical scheme of the present invention is summarized as follows:
A kind of maitake mushroom mycelia wet method ultra micro wall-breaking method comprises the steps:
(1) acquisition of Grifola frondosa fungal fermented filtrate and pre-treatment: the Grifola frondosa slant strains spread cultivation step by step obtains the Grifola frondosa fungal fermented filtrate, and fermented liquid is heated to 70～100 ℃, keeps 10～30 minutes, cools to 50～60 ℃ then;
(2) wet method one-level broken wall: the gap of adjusting colloidal mill stator and rotor is 0.5～1 micron, utilize its shear action that the mycelium in the fermented liquid is carried out the one-level broken wall treatment, the colloidal mill flow is 0.1～1 ton/hour, make hyphostoma particle degree behind the broken wall be 0.4～0.8 micron account for 70%;
(3) wet method secondary ultra micro broken wall: adopt particle size after cracking at 0.1～0.5 micron high pressure homogenizer, utilize its high pressure release force, hole effect, the isodynamic effect of shearing, mycelium in the fermented liquid is carried out the ultra micro broken wall, adjusting high pressure homogenizer pressure is 60～100Mpa, high-pressure homogeneous flow is 0.1～1 ton/hour, make handle the back raw meal particle size be 40～100 nanometers account for 80%;
Preferred 75～90 ℃ of Grifola gigantea (Pers.) Piat. Fermented liquid pre-treatment Heating temperature, the time kept 20 minutes, cooled to 52～58 ℃ and was advisable.
The adjusting play of colloidal mill stator and rotor preferably is adjusted to 0.5～0.7 micron, and the colloidal mill flow is 0.5 ton/hour, the hyphostoma particle degree behind the broken wall be 0.4～0.6 micron account for 70%.
High pressure homogenizer pressure preferably is adjusted to 80～90Mpa, 0.1～0.5 ton/hour of high-pressure homogeneous optimal flux, make handle the back raw meal particle size be 40～80 nanometers account for 80%.
Advantage of the present invention: it is the high pressure homogenizer combination at the 0.1-0.5 micron of 0.5～1 micron colloidal mill and grinding particle size that the present invention selects grinding particle size for use, adopt two-stage wet method ultra micro technology for broken wall that maitake mushroom mycelia in the fermented liquid is carried out the ultra micro broken wall treatment, raw meal particle size 80% has reached nano level after making the mycelium broken wall, stripping of mycelium functional factor full price and efficient the utilization have been realized, by adopting the combination of colloidal mill and high pressure homogenizer, reached the nano level crushing effect of maitake mushroom mycelia.With the fermented liquid behind the ultra micro broken wall is base-material, and the extraction raw material that both can be used as effective constituents such as grifolan also can be machined directly to the liquid or solid deep processed product.
The Grifola frondosa bacterial classification that is adopted among the present invention is purchased in Chinese industrial microbial strains preservation center, bacterium number: CICC 14007.
Xu Jin hall volume " Chinese medicinal fungi " is seen in the cultivation of Grifola frondosa fungi liquid submerged fermentation liquid among the present invention.
Embodiment
The following examples can make those skilled in the art more fully understand the present invention, but do not limit the present invention in any way.
Embodiment 1:(is an example with 1 ton of fermented liquid)
1, tank fermentation method is cultivated the process of Grifola frondosa fungal fermented filtrate:
(1) one-level is shaken a bottle culture of strains: the Grifola frondosa slant strains is inserted first order seed shake in the bottle, it is 500 milliliters of triangular flasks that first order seed shakes bottle, 100 milliliters of substratum loading amounts, 60 rev/mins of rotary shaking tables, 24 ℃ of culture temperature, incubation time 50 hours;
(2) secondary shakes a bottle culture of strains: one-level is shaken bottle bacterial classification insert secondary seed with 10% inoculum size and shake in the bottle, its culture condition is identical with first order seed;
(3) three grades are shaken a bottle culture of strains: secondary is shaken bottle bacterial classification insert three grades of seeds with 10% inoculum size and shake in the bottle, it is 5000 milliliters that three grades of seeds shake bottle, 1500 milliliters of substratum loading amounts, 100 rev/mins of rotary shaking tables, 24 ℃ of culture temperature, incubation time 50 hours;
(4) first class seed pot spawn culture: shake bottle bacterial classification with three grades and insert in the first class seed pot, the first order seed tank volume is 50 liters, 35 liters of substratum loading amounts, inoculum size 10%, 24 ℃ of culture temperature, 120 rev/mins of stirring velocitys, ventilation (V/V) 1: 0.5, tank pressure 0.05Mpa, incubation time 48 hours;
(5) secondary seed jar spawn culture: the bacterial classification that first class seed pot is cultivated inserts in the secondary seed jar, the secondary seed tank volume is 300 liters, 200 liters of substratum loading amounts, inoculum size 15%, 24 ℃ of culture temperature, 120 rev/mins of stirring velocitys, ventilation (V/V) 1: 0.5, tank pressure 0.05Mpa, incubation time 48 hours;
(6) fermentor cultivation: the bacterial classification that the secondary seed jar is cultivated inserts in 1.5 tons the fermentor tank, 1 ton of substratum loading amount, inoculum size 20%, 25 ℃ of culture temperature, 100 rev/mins of stirring velocitys, ventilation (V/V) 1: 0.5, tank pressure 0.05Mpa, incubation time 72 hours, fermentation ends.
2, fermented liquid wet method ultra micro broken wall:
(1) Grifola frondosa fungal fermented filtrate pre-treatment: after fermentation ends, fermented liquid is heated to 90 ℃, kept 20 minutes, cool to 50 ℃ then;
(2) wet method one-level broken wall: 50 ℃ fermented liquids are input in the colloidal mill with in-line pump, the gap of adjusting colloidal mill stator and rotor is 1 micron, the colloidal mill flow is 0.5 ton/hour, carries out mycelial one-level broken wall treatment, and the granularity behind the mycelium broken wall is 0.4～0.8 micron and accounts for 70%;
(3) wet method secondary ultra micro broken wall: the adjustment homogenization pressure is 60Mpa, high-pressure homogeneous flow is 0.4 ton/hour, utilize high pressure homogenizer to carry out mycelial ultra micro broken wall treatment, sampling detects through particle-size analyzer, sporoderm-broken rate is 100%, raw meal particle size be 40～100 nanometers account for 80%, realized the wet method ultra micro broken wall of maitake mushroom mycelia, the extraction raw material that both can be used as effective constituent such as grifolan through the maitake mushroom mycelia fermented liquid behind the ultra micro broken wall also can be machined directly to the liquid or solid deep processed product.
Slant medium consists of in the present embodiment: potato (liquor) 20%, glucose 2%, agar 2.0%.
First order seed, secondary seed, three grades of used substratum compositions of seed, first class seed pot, secondary seed jar and fermentative production are in the present embodiment: sucrose 5%, glucose 2%, defatted soybean protein powder 0.5%, potassium primary phosphate 0.2%, sal epsom 0.1%, PH6.0.
In this example during fermentation ends: pH value 4.0, mycelia weight in wet base 22%.
Granularity adopts laser particle size analyzer to carry out the mensuration of granularity in the fermented liquid in the present embodiment.
Embodiment 2:(is an example with 1 ton of fermented liquid)
1, tank fermentation method is cultivated the process of Grifola frondosa fungal fermented filtrate: with embodiment 1.
2, fermented liquid wet method ultra micro broken wall:
(1) Grifola frondosa fungal fermented filtrate pre-treatment: after fermentation ends, fermented liquid is heated to 100 ℃, kept 10 minutes, cool to 60 ℃ then;
(2) wet method one-level broken wall: 60 ℃ fermented liquids are input in the colloidal mill with in-line pump, the gap of adjusting colloidal mill stator and rotor is 0.5 micron, the colloidal mill flow is 0.1 ton/hour, carry out mycelial one-level broken wall treatment, the granularity behind the mycelium broken wall is 0.4～0.5 micron and accounts for 70%;
(3) wet method secondary ultra micro broken wall: the adjustment homogenization pressure is 80Mpa, high-pressure homogeneous flow is 0.1 ton/hour, utilizes high pressure homogenizer to carry out mycelial ultra micro broken wall treatment, and sampling detects through particle-size analyzer, sporoderm-broken rate is 100%, raw meal particle size be 40～90 nanometers account for 80%.
Embodiment 3:(is an example with 1 ton of fermented liquid)
1, tank fermentation method is cultivated the process of Grifola frondosa fungal fermented filtrate: with embodiment 1.
2, fermented liquid wet method ultra micro broken wall:
(1) Grifola frondosa fungal fermented filtrate pre-treatment: after fermentation ends, fermented liquid is heated to 70 ℃, kept 30 minutes, cool to 52 ℃ then;
(2) wet method one-level broken wall: 52 ℃ fermented liquids are input in the colloidal mill with in-line pump, the gap of adjusting colloidal mill stator and rotor is 0.7 micron, the colloidal mill flow is 1 ton/hour, carries out mycelial one-level broken wall treatment, and the granularity behind the mycelium broken wall is 0.4～0.6 micron and accounts for 70%;
(3) wet method secondary ultra micro broken wall: the adjustment homogenization pressure is 100Mpa, high-pressure homogeneous flow is 0.5 ton/hour, utilizes high pressure homogenizer to carry out mycelial ultra micro broken wall treatment, and sampling detects through particle-size analyzer, sporoderm-broken rate is 100%, raw meal particle size be 40～70 nanometers account for 80%.
Embodiment 4:(is an example with 1 ton of fermented liquid)
1, tank fermentation method is cultivated the process of Grifola frondosa fungal fermented filtrate: with embodiment 1.
2, fermented liquid wet method ultra micro broken wall:
(1) Grifola frondosa fungal fermented filtrate pre-treatment: after fermentation ends, fermented liquid is heated to 75 ℃, kept 30 minutes, cool to 58 ℃ then;
(2) wet method one-level broken wall: 58 ℃ fermented liquids are input in the colloidal mill with in-line pump, the gap of adjusting colloidal mill stator and rotor is 0.7 micron, the colloidal mill flow is 1 ton/hour, carries out mycelial one-level broken wall treatment, and the granularity behind the mycelium broken wall is 0.4～0.6 micron and accounts for 70%;
(3) wet method secondary ultra micro broken wall: the adjustment homogenization pressure is 90Mpa, high-pressure homogeneous flow is 0.5 ton/hour, utilizes high pressure homogenizer to carry out mycelial ultra micro broken wall treatment, and sampling detects through particle-size analyzer, sporoderm-broken rate is 100%, raw meal particle size be 40～80 nanometers account for 80%.
Claims (4)
1. a maitake mushroom mycelia wet method ultra micro wall-breaking method comprises the steps:
(1) acquisition of Grifola frondosa fungal fermented filtrate and pre-treatment: the Grifola frondosa slant strains spread cultivation step by step obtains the Grifola frondosa fungal fermented filtrate, and fermented liquid is heated to 70～100 ℃, keeps 10～30 minutes, cools to 50～60 ℃ then;
(2) wet method one-level broken wall: the gap of adjusting colloidal mill stator and rotor is 0.5～1 micron, and the colloidal mill flow is 0.1～1 ton/hour, make hyphostoma particle degree behind the broken wall be 0.4～0.8 micron account for 70%;
(3) wet method secondary ultra micro broken wall: adjusting high pressure homogenizer pressure is 60～100Mpa, and high-pressure homogeneous flow is 0.1～1 ton/hour, make handle the back raw meal particle size be 40～100 nanometers account for 80%.
2. a kind of maitake mushroom mycelia wet method ultra micro wall-breaking method according to claim 1 is characterized in that described Grifola gigantea (Pers.) Piat. Fermented liquid pre-treatment Heating temperature is 75～90 ℃, keeps 20 minutes, cools to 52～58 ℃.
3. a kind of maitake mushroom mycelia wet method ultra micro wall-breaking method according to claim 1, the adjusting play that it is characterized in that described colloidal mill stator and rotor is 0.5～0.7 micron, the colloidal mill flow is 0.5 ton/hour, the hyphostoma particle degree behind the broken wall be 0.4～0.6 micron account for 70%.
4. a kind of maitake mushroom mycelia wet method ultra micro wall-breaking method according to claim 1 is characterized in that described high pressure homogenizer pressure is 80～90Mpa, 0.1～0.5 ton/hour of high-pressure homogeneous flow, make handle the back raw meal particle size be 40～80 nanometers account for 80%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102433215A (en) * | 2011-09-22 | 2012-05-02 | 厦门汇盛生物有限公司 | Method of extracting grease from fungi or algae through physical wall-breaking |
| CN103549409A (en) * | 2013-11-15 | 2014-02-05 | 哈尔滨艾克尔食品科技有限公司 | Preparation method of Yun Xun electuary |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102433215A (en) * | 2011-09-22 | 2012-05-02 | 厦门汇盛生物有限公司 | Method of extracting grease from fungi or algae through physical wall-breaking |
| CN103549409A (en) * | 2013-11-15 | 2014-02-05 | 哈尔滨艾克尔食品科技有限公司 | Preparation method of Yun Xun electuary |
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