CN103725403A - Method for extracting engraulis japonicus oil by two-enzyme method - Google Patents
Method for extracting engraulis japonicus oil by two-enzyme method Download PDFInfo
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- CN103725403A CN103725403A CN201310650254.3A CN201310650254A CN103725403A CN 103725403 A CN103725403 A CN 103725403A CN 201310650254 A CN201310650254 A CN 201310650254A CN 103725403 A CN103725403 A CN 103725403A
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Abstract
The invention relates to a method for extracting engraulis japonicus oil by a two-enzyme method. The method comprises the following steps: selecting frozen and thawed fresh engraulis japonicas as a raw material, cleaning, smashing, adding water to dilute, regulating the pH value, adding alkaline protease, and controlling the enzymolysis condition to further carry out primary enzymolysis; regulating the pH value, adding pepsin, controlling the enzymolysis condition to further carry out secondary enzymolysis; separating after enzyme deactivation to obtain crude fish oil; performing deacidification, decoloration and deodorization treatment on the crude fish oil to obtain refined fish oil. The method is reasonable in process, advanced in technology, feasible in operation, and high in fish oil yield and purity.
Description
Technical field
The present invention relates to the preparation of grease, especially a kind of double-enzyme method extracting method of anchovy oil.
Background technology
Anchovy is that one way of life is at ocean, temperate zone small fish at the middle and upper levels, be distributed widely in Bohai and Yellow Seas and the East Sea of China, being the single fingerling of planting fish stock biomass maximum in the yellow East Sea, is the food organism of other economic fish, is also the keystone species in the food chain of the yellow East Sea.More than 20 % of the total catches of anchovy annual production Zeng Gaoda marine fishes, be that year amount of fishing comes one of marine fishes of first 10.At present, the manufacture raw material of fish meal during anchovy is main, average every 5.3 tons of anchovies can be made one ton of fish meal.And oil is the byproduct of the processing of fish meal, Anchovy Oil contains abundant EPA and DHA, and polyunsaturated fatty acid has different physiological roles, can be widely used in food, medicine and culture fishery.The traditional preparation method of anchovy oil by anchovy and waste thereof through boiling, squeeze and be isolated, there is the deficiency that yield is low.
Therefore, researching and developing a kind of method that adopts zymin enzymolysis anchovy to produce fish oil, to improve oily yield, and solve in fish oil and have variegated and stink, is an important channel that effectively utilizes anchovy resource and improve anchovy added value.
Summary of the invention
In order to overcome available technology adopting boiling, squeeze and be isolated anchovy oil, exist the deficiency that yield is low, the invention provides a kind of double-enzyme method extracting method of anchovy oil, the method technique rationally, advanced technology, operation feasible, fish oil yield and purity high.
The technical solution adopted for the present invention to solve the technical problems is: a kind of double-enzyme method extracting method of anchovy oil, is characterized in that: process following process steps:
Choosing and processing that to choose fresh anchovy freezing and that thaw be raw material of A, raw material, after rinsing well, carries out pulverization process with pulverizer with clear water;
B, primary enzymolysis add water by the anchovy after pulverization process and mix, feed liquid mass ratio 1: 3～5, regulate pH value 8～9, add Sumizyme MP, the enzyme dosage of described Sumizyme MP is 1～2 % of anchovy raw materials quality, control 45～55 ℃ of temperature, enzymolysis time 2～3h, obtains anchovy primary enzymolysis liquid;
C, secondary enzymolysis regulate pH value 2～3 by resulting primary enzymolysis liquid, add stomach en-, described pepsic enzyme dosage is 0.25～1.25 % of anchovy raw materials quality, controls 35～45 ℃ of temperature, enzymolysis time 2.5～4.5 h, obtain anchovy secondary enzymolysis liquid;
It is 4～5 that D, the enzyme that goes out regulate pH value by resulting secondary enzymolysis liquid, the water-bath enzyme 20～30min that goes out under 90～100 ℃ of temperature parts;
E, separation adopt whizzer to carry out solid-liquid separation the anchovy secondary enzymolysis liquid going out after enzyme, obtain fluid mixture; Standing to fluid demixing, get upper strata oily liquids, obtain crude fish oil;
F, depickling are processed resulting crude fish oil are heated to 40～50 ℃, add the sodium hydroxide solution that crude fish oil quality 0.5～1%, concentration are 20～25%, after stirring, be warming up to 60～65 ℃, Heat preservation 20～30min, takes out, cooling, adopt whizzer to carry out solid-liquid separation, remove precipitation, obtain depickling fish oil;
G, decolouring are processed resulting depickling fish oil are heated to 50～60 ℃, add the gac of depickling fish oil quality 1-3%, stir decolouring 20～30 min, adopt whizzer to carry out solid-liquid separation, obtain decolouring fish oil;
H, deodorization are processed resulting decolouring fish oil under 60～70 ℃ of conditions, adopt Rotary Evaporators, control vacuum tightness 0.05～0.06Mpa, and vacuum deodorization is processed 20～30 minutes, obtains refined fish oil.
The present invention is that to choose fresh anchovy freezing and that thaw be raw material, cleans, and pulverizes, and adds water and carries out dilution process, and adjust pH, adds Sumizyme MP, and controlled enzymatic hydrolysis condition is carried out primary enzymolysis; Adjust pH adds stomach en-, and controlled enzymatic hydrolysis condition is carried out secondary enzymolysis; Go out separated after enzyme, obtain crude fish oil; Crude fish oil is carried out to depickling, decolouring, deodorization processing, obtain refined fish oil.The present invention adopts zymin to be hydrolyzed to the protein of anchovy, thereby destroys protein and fatty marriage relation, discharges grease.Meanwhile, two enzymes that the present invention adopts carry out secondary hydrolysis to the protein of anchovy successively, and proteolysis degree is high, and oil yield reaches optimum quantity.The depickling of then carrying out, decolouring, deodorization are processed, and the color of its fish oil, state, smell have obtained obvious improvement, and on not impact of the polyunsaturated fatty acid in fish oil.The double-enzyme method extracting method technique of anchovy oil of the present invention rationally, advanced technology, operation feasible, fish oil yield and purity high.
Embodiment
Below in conjunction with embodiment, the present invention will be further described.
embodiment mono-
A double-enzyme method extracting method for anchovy oil, process following process steps:
Choosing and processing that to choose fresh anchovy 100kg freezing and that thaw be raw material of A, raw material, after rinsing well, carries out pulverization process with pulverizer with clear water;
B, primary enzymolysis add water by the anchovy after pulverization process and mix, and feed liquid mass ratio 1: 4 regulates pH value 8.5, add Sumizyme MP, the enzyme dosage of Sumizyme MP is 1.5% of anchovy raw materials quality, controls temperature 50 C, enzymolysis time 2.5h, obtains anchovy primary enzymolysis liquid;
C, secondary enzymolysis regulate pH value 2.5 by resulting primary enzymolysis liquid, add stomach en-, and pepsic enzyme dosage is 1% of anchovy raw materials quality, control 40 ℃ of temperature, and enzymolysis time 3h, obtains anchovy secondary enzymolysis liquid;
It is 4 that D, the enzyme that goes out regulate pH value by resulting secondary enzymolysis liquid, the water-bath enzyme 25min that goes out under 95 ℃ of temperature parts;
E, separation adopt whizzer to carry out solid-liquid separation the anchovy secondary enzymolysis liquid going out after enzyme, obtain fluid mixture; Standing to fluid demixing, get upper strata oily liquids, obtain crude fish oil 72kg;
F, depickling are processed resulting crude fish oil are heated to 45 ℃, add the sodium hydroxide solution that crude fish oil quality 0.8%, concentration are 23%, after stirring, are warming up to 60 ℃, Heat preservation 30 min, take out, cooling, adopt whizzer to carry out solid-liquid separation, remove precipitation, obtain depickling fish oil;
G, decolouring are processed resulting depickling fish oil are heated to 55 ℃, add the gac of depickling fish oil quality 2%, stir decolouring 25min, adopt whizzer to carry out solid-liquid separation, obtain decolouring fish oil;
H, deodorization are processed resulting decolouring fish oil under 65 ℃ of conditions, adopt Rotary Evaporators, control vacuum tightness 0.05Mpa, and vacuum deodorization is processed 25 minutes, obtains refined fish oil 61kg.
embodiment bis-
A double-enzyme method extracting method for anchovy oil, process following process steps:
Choosing and processing that to choose fresh anchovy 200kg freezing and that thaw be raw material of A, raw material, after rinsing well, carries out pulverization process with pulverizer with clear water;
B, primary enzymolysis add water by the anchovy after pulverization process and mix, and feed liquid mass ratio 1: 5 regulates pH value 9, add Sumizyme MP, the enzyme dosage of Sumizyme MP is 2% of anchovy raw materials quality, controls temperature 45 C, enzymolysis time 2h, obtains anchovy primary enzymolysis liquid;
C, secondary enzymolysis regulate pH value 3 by resulting primary enzymolysis liquid, add stomach en-, and pepsic enzyme dosage is 1.25% of anchovy raw materials quality, control 35 ℃ of temperature, and enzymolysis time 2.5h, obtains anchovy secondary enzymolysis liquid;
It is 4.5 that D, the enzyme that goes out regulate pH value by resulting secondary enzymolysis liquid, the water-bath enzyme 20min that goes out under 100 ℃ of temperature parts;
E, separation adopt whizzer to carry out solid-liquid separation the anchovy secondary enzymolysis liquid going out after enzyme, obtain fluid mixture; Standing to fluid demixing, get upper strata oily liquids, obtain crude fish oil 135kg;
F, depickling are processed resulting crude fish oil are heated to 50 ℃, add the sodium hydroxide solution that crude fish oil quality 0.5%, concentration are 23%, after stirring, are warming up to 65 ℃, Heat preservation 20min, takes out, cooling, adopt whizzer to carry out solid-liquid separation, remove precipitation, obtain depickling fish oil;
G, decolouring are processed resulting depickling fish oil are heated to 60 ℃, add the gac of depickling fish oil quality 3%, stir decolouring 20min, adopt whizzer to carry out solid-liquid separation, obtain decolouring fish oil;
H, deodorization are processed resulting decolouring fish oil under 70 ℃ of conditions, adopt Rotary Evaporators, control vacuum tightness 0.06Mpa, and vacuum deodorization is processed 20 minutes, obtains refined fish oil 113kg.
embodiment tri-
A double-enzyme method extracting method for anchovy oil, process following process steps:
Choosing and processing that to choose fresh anchovy 100kg freezing and that thaw be raw material of A, raw material, after rinsing well, carries out pulverization process with pulverizer with clear water;
B, primary enzymolysis add water by the anchovy after pulverization process and mix, and feed liquid mass ratio 1: 3 regulates pH value 8, add Sumizyme MP, the enzyme dosage of Sumizyme MP is 1% of anchovy raw materials quality, controls 55 ℃ of temperature, enzymolysis time 3h, obtains anchovy primary enzymolysis liquid;
C, secondary enzymolysis regulate pH value 2 by resulting primary enzymolysis liquid, add stomach en-, and pepsic enzyme dosage is 0.25% of anchovy raw materials quality, control temperature 45 C, and enzymolysis time 4.5 h, obtain anchovy secondary enzymolysis liquid;
It is 4 that D, the enzyme that goes out regulate pH value by resulting secondary enzymolysis liquid, the water-bath enzyme 30min that goes out under 90 ℃ of temperature parts;
E, separation adopt whizzer to carry out solid-liquid separation the anchovy secondary enzymolysis liquid going out after enzyme, obtain fluid mixture; Standing to fluid demixing, get upper strata oily liquids, obtain crude fish oil 77.3kg;
F, depickling are processed resulting crude fish oil are heated to 40 ℃, add the sodium hydroxide solution that crude fish oil quality 1%, concentration are 25%, after stirring, are warming up to 60 ℃, Heat preservation 25min, takes out, cooling, adopt whizzer to carry out solid-liquid separation, remove precipitation, obtain depickling fish oil;
G, decolouring are processed resulting depickling fish oil are heated to 50 ℃, add the gac of depickling fish oil quality 1%, stir decolouring 30min, adopt whizzer to carry out solid-liquid separation, obtain decolouring fish oil;
H, deodorization are processed resulting decolouring fish oil under 60 ℃ of conditions, adopt Rotary Evaporators, control vacuum tightness 0.05-0.06Mpa, and vacuum deodorization is processed 20-30 minute, obtains refined fish oil 61.84kg.
Claims (1)
1. a double-enzyme method extracting method for anchovy oil, is characterized in that: through following process steps:
Choosing and processing that to choose fresh anchovy freezing and that thaw be raw material of A, raw material, after rinsing well, carries out pulverization process with pulverizer with clear water;
B, primary enzymolysis add water by the anchovy after pulverization process and mix, feed liquid mass ratio 1: 3～5, regulate pH value 8～9, add Sumizyme MP, the enzyme dosage of described Sumizyme MP is 1～2 % of anchovy raw materials quality, control 45～55 ℃ of temperature, enzymolysis time 2～3h, obtains anchovy primary enzymolysis liquid;
C, secondary enzymolysis regulate pH value 2～3 by resulting primary enzymolysis liquid, add stomach en-, described pepsic enzyme dosage is 0.25～1.25 % of anchovy raw materials quality, controls 35～45 ℃ of temperature, enzymolysis time 2.5～4.5 h, obtain anchovy secondary enzymolysis liquid;
It is 4～5 that D, the enzyme that goes out regulate pH value by resulting secondary enzymolysis liquid, the water-bath enzyme 20～30min that goes out under 90～100 ℃ of temperature parts;
E, separation adopt whizzer to carry out solid-liquid separation the anchovy secondary enzymolysis liquid going out after enzyme, obtain fluid mixture; Standing to fluid demixing, get upper strata oily liquids, obtain crude fish oil;
F, depickling are processed resulting crude fish oil are heated to 40～50 ℃, add the sodium hydroxide solution that crude fish oil quality 0.5～1%, concentration are 20～25%, after stirring, be warming up to 60～65 ℃, Heat preservation 20～30min, takes out, cooling, adopt whizzer to carry out solid-liquid separation, remove precipitation, obtain depickling fish oil;
G, decolouring are processed resulting depickling fish oil are heated to 50～60 ℃, add the gac of depickling fish oil quality 1-3%, stir decolouring 20～30 min, adopt whizzer to carry out solid-liquid separation, obtain decolouring fish oil;
H, deodorization are processed resulting decolouring fish oil under 60～70 ℃ of conditions, adopt Rotary Evaporators, control vacuum tightness 0.05～0.06Mpa, and vacuum deodorization is processed 20～30 minutes, obtains refined fish oil.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105670781A (en) * | 2016-01-21 | 2016-06-15 | 华南农业大学 | Method of extracting decapterus maruadsi oil through low-temperature continuous phase transition |
| CN106753755A (en) * | 2017-01-23 | 2017-05-31 | 石狮市华宝海洋生物医药有限公司 | A kind of preparation technology of isoamyl ester type fish oil |
| CN106800969A (en) * | 2017-01-23 | 2017-06-06 | 石狮市华宝海洋生物医药有限公司 | It is a kind of to be segmented the technique that conbined enzymolysis extract fish oil from cuttlefish internal organ |
| CN106819955A (en) * | 2017-01-23 | 2017-06-13 | 石狮市华宝海洋生物医药有限公司 | A kind of preparation technology of the stable type fish oil of decoloration deodorization |
| CN112625786A (en) * | 2020-12-31 | 2021-04-09 | 浙江工业大学 | Preparation method of tuna oil |
| CN114057574A (en) * | 2021-12-03 | 2022-02-18 | 浙江工商大学 | Method for preparing high-purity EPA ethyl ester |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105670781A (en) * | 2016-01-21 | 2016-06-15 | 华南农业大学 | Method of extracting decapterus maruadsi oil through low-temperature continuous phase transition |
| CN105670781B (en) * | 2016-01-21 | 2019-08-16 | 华南农业大学 | The method and decapterus maruadsi fish oil of decapterus maruadsi fish oil are extracted in a kind of low temperature continuous phase transistion |
| CN106753755A (en) * | 2017-01-23 | 2017-05-31 | 石狮市华宝海洋生物医药有限公司 | A kind of preparation technology of isoamyl ester type fish oil |
| CN106800969A (en) * | 2017-01-23 | 2017-06-06 | 石狮市华宝海洋生物医药有限公司 | It is a kind of to be segmented the technique that conbined enzymolysis extract fish oil from cuttlefish internal organ |
| CN106819955A (en) * | 2017-01-23 | 2017-06-13 | 石狮市华宝海洋生物医药有限公司 | A kind of preparation technology of the stable type fish oil of decoloration deodorization |
| CN106800969B (en) * | 2017-01-23 | 2020-06-05 | 石狮市华宝海洋生物医药有限公司 | Process for extracting fish oil from internal organs of cuttlefish by segmented compound enzymolysis method |
| CN112625786A (en) * | 2020-12-31 | 2021-04-09 | 浙江工业大学 | Preparation method of tuna oil |
| CN114057574A (en) * | 2021-12-03 | 2022-02-18 | 浙江工商大学 | Method for preparing high-purity EPA ethyl ester |
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Application publication date: 20140416 |