CN105385715A - Method improving antioxidation of unsaturated fatty acid - Google Patents
Method improving antioxidation of unsaturated fatty acid Download PDFInfo
- Publication number
- CN105385715A CN105385715A CN201510824311.4A CN201510824311A CN105385715A CN 105385715 A CN105385715 A CN 105385715A CN 201510824311 A CN201510824311 A CN 201510824311A CN 105385715 A CN105385715 A CN 105385715A
- Authority
- CN
- China
- Prior art keywords
- unsaturated fatty
- fatty acids
- fatty acid
- rhizoma curcumae
- curcumae longae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention belongs to the field of microbial fermentation and provides a method improving antioxidation of unsaturated fatty acid. Certain amount of turmeric extract is added in a microbial fermentation process, so that the antioxidation of the unsaturated fatty acid is improved, and the oxidating induction time is prolonged by 0.5-2h. By adopting the method, without changing the conditions such as fermentation temperature, ventilation, and fermentation time, the shelf life of the unsaturated fatty acid can be prolonged.
Description
Technical field:
The present invention relates to microbe oil fermentation field.Relate to specifically and add a certain amount of Rhizoma Curcumae Longae extract in microbe oil fermentation process, the unsaturated fatty acids antioxidant property in grease can be improved.
Background technology:
Microbial oil (microbialoils) is also known as Unicell Oils and Fats (singlecelloil, SCO), be utilize that carbohydrate, hydrocarbon polymer and common grease are carbon source under certain condition by microorganisms such as yeast, mould, bacterium and algae, nitrogenous source, be aided with the grease of production of inorganic.Containing some specific unsaturated fatty acids in microbial oil, as ARA, DHA, EPA, linolic acid, squalene etc., microorganism now for the production of polyunsaturated fatty acid is mainly algae, bacterium and fungi, because Bacterial production is low, so mainly concentrate on algae and fungi at present.Unsaturated fatty acids can keep the relative flow of cytolemma, to ensure the normal physiological function of cell.And make cholesterol esterification, reduce blood cholesterol and triglyceride level.Unsaturated fatty acids is the precursor substance of prostaglandin(PG) and thromboxane in synthesized human.Can blood viscosity be reduced, improve blood microcirculation.The activity of brain cell can be improved, memory and thinking ability.
The vital role of unsaturated fatty acids (Polyunsaturatedfattyacids, PUFAs) to human health is more and more subject to people's attention.Adopt in fermentative Production unsaturated fatty acids process, improve the antioxidant property of grease, can extend the quality guaranteed period of grease, purport of the present invention adds a certain amount of Rhizoma Curcumae Longae extract during the fermentation, can improve unsaturated fatty acids antioxidant property, oxidation induction time extends 0.5h-2h.
Summary of the invention:
(1) technical problem that will solve
The object of the present invention is to provide and add a certain amount of Rhizoma Curcumae Longae extract in fermentation process, unsaturated fatty acids antioxidant property can be improved, adopt described method can not also change the conditions such as leavening temperature, air flow, fermentation time.
(2) summary of the invention
For realizing the object of the invention, the method for raising unsaturated fatty acids antioxidant property provided by the invention, is characterized in that adding Rhizoma Curcumae Longae extract in fermentation process.
Preferably, the method for described raising unsaturated fatty acids antioxidant property, adds 0.2 μ g/ml-20 μ g/ml Rhizoma Curcumae Longae extract in fermentation process.
Preferably, the method of described raising unsaturated fatty acids antioxidant property, adopts and splits the fermentable such as kettle algae (Schizochytriumsp.), I Ken Shi kettle algae (Ulkeniaamoeboida), Kou Shi Crypthecodinium cohnii (Crypthecodiniumcohnii), Mortierella alpine enzyme (Mortierellaalpina) cultivation production DHA grease and ARA grease.
Microbe oil fermentation is produced usually to adopt and is split the cultivation of the fermentable such as kettle algae (Schizochytriumsp.), I Ken Shi kettle algae (Ulkeniaamoeboida), Kou Shi Crypthecodinium cohnii (Crypthecodiniumcohnii), Mortierella alpine enzyme (Mortierellaalpina), and used medium can containing suitable carbon source, nitrogenous source, inorganic salt and VITAMIN.Described substratum is with glucose or glycerine or fructose for carbon source, with a kind of in yeast extract, peptone, casein hydrolysate, L-glutamic acid, corn steep liquor, soybean protein or mixture for nitrogenous source.Inorganic salt are sodium-chlor, magnesium sulfate, calcium chloride, Repone K, potassium primary phosphate.VITAMIN be VITMAIN B1, vitamin B6, vitamin B12 one or more.
The invention has the advantages that:
Change the conditions such as leavening temperature, air flow, fermentation time.Add a certain amount of Rhizoma Curcumae Longae extract in fermenting process, can improve unsaturated fatty acids antioxidant property, oxidation induction time extends 0.5h-2h.
Embodiment
Embodiment one:
Culture medium prescription (g/100ml)
Sodium-chlor 1.24g Repone K 0.07g calcium chloride 0.035g
Magnesium sulfate 0.7g potassium primary phosphate 0.2g Sodium Glutamate 4.2g
Corn steep liquor 3.5g glucose 7.5g vitamins B
10.003g
Vitamins B
60.005g adds water to volume required, PH nature.
Contrast experiment is carried out with above-mentioned formula, employing is split kettle algae (Schizochytriumsp.) slant strains and is carried out liquid submerged culture, liquid shaking bottle enlarged culturing, first order seed is cultivated, secondary seed is cultivated and three grade fermemtation is cultivated, grease is extracted when adding the experimental group 90h of Rhizoma Curcumae Longae extract 0.001g, the oxidation induction time of 80 DEG C of greases is detected with Rancimat determinator, detected result is 15.5h, grease is extracted when not adding the experimental group 90h of Rhizoma Curcumae Longae extract, the oxidation induction time of 80 DEG C of greases is detected with Rancimat determinator, detected result is 13.7h, experimental data shows, Rhizoma Curcumae Longae extract group comparatively comparative group oxidation induction time extends 1.8h.
Embodiment two:
(1) strain activation and culture
Slant culture based formulas is as follows:
Potato 30g, glucose 2-4g, agar 1.5-2g, water 100ml;
The activation of Mortierella alpine trichoderma strain adopts slant strains to cultivate, culture temperature 28-30 DEG C, incubation time 7-10 days;
(2) shake-flask culture
Shake-flask culture based formulas volumetric concentration is as follows:
Glucose 20-40g/L, yeast powder 2.5-5g/L;
Slant strains is adopted to carry out liquid submerged culture, culture temperature 28-30 DEG C, rotating speed 140-200rpm, incubation time 7-10 days;
(3) fermention medium volumetric concentration is as follows:
Glucose 30-60g/L, corn steep liquor 50-60g/L;
1. one grade fermemtation is cultivated: by step (2) in shaking flask strain transfer enter one and be equipped with in the fermentor tank of primary-seed medium, inoculum size is 0.1-1%, culture temperature 28-30 DEG C, and tank pressure controls at 0.015-0.05MPa, rotating speed 140-200rpm, time 2-5 days;
2. second order fermentation is cultivated: the strain transfer in first class seed pot is entered one and is equipped with in the fermentor tank of secondary seed medium, inoculum size is 1-10%, culture temperature 28-30 DEG C, and tank pressure controls at 0.015-0.05MPa, rotating speed 140-200rpm, time 2-5 days;
3. three grade fermemtation is cultivated: the strain transfer of secondary seed tank kind is entered one and is equipped with in the fermentor tank of three grade fermemtation substratum, inoculum size is 1-10%, culture temperature 28-30 DEG C, and tank pressure controls at 0.015-0.05MPa, and rotating speed 140-200rpm, ferments.Rhizoma Curcumae Longae extract 0.2mg/L is added at fermentation 78h, grease is extracted during Rhizoma Curcumae Longae extract group 168h, the oxidation induction time of 80 DEG C of greases is detected with Rancimat determinator, detected result is 26h, extract grease when not adding the experimental group 168h of Rhizoma Curcumae Longae extract, detect the oxidation induction time of 80 DEG C of greases with Rancimat determinator, detected result is 24h, experimental data shows, and Rhizoma Curcumae Longae extract group comparatively comparative group unsaturated fatty acids oxidation induction time extends 1.8h.
Claims (3)
1. improve the method for unsaturated fatty acids antioxidant property, it is characterized in that adding Rhizoma Curcumae Longae extract in fermentation process.
2. the method for raising unsaturated fatty acids antioxidant property according to claim 1, is characterized in that adding 0.2 μ g/ml-20 μ g/ml Rhizoma Curcumae Longae extract in fermenting process.
3. the method for raising unsaturated fatty acids antioxidant property according to claim 1 and 2, is characterized in that employing is split the fermentable such as kettle algae (Schizochytriumsp.), I Ken Shi kettle algae (Ulkeniaamoeboida), Kou Shi Crypthecodinium cohnii (Crypthecodiniumcohnii), Mortierella alpine enzyme (Mortierellaalpina) and cultivated production DHA grease and ARA grease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510824311.4A CN105385715A (en) | 2015-11-24 | 2015-11-24 | Method improving antioxidation of unsaturated fatty acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510824311.4A CN105385715A (en) | 2015-11-24 | 2015-11-24 | Method improving antioxidation of unsaturated fatty acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105385715A true CN105385715A (en) | 2016-03-09 |
Family
ID=55418494
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510824311.4A Pending CN105385715A (en) | 2015-11-24 | 2015-11-24 | Method improving antioxidation of unsaturated fatty acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105385715A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112662712A (en) * | 2021-01-26 | 2021-04-16 | 湖北福星生物科技有限公司 | Microbial-derived DHA grease, preparation method thereof and functional food |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1284277A (en) * | 1999-07-19 | 2001-02-21 | 大城清利 | Edible oil Radix Cureumae and its making method |
| CN1986822A (en) * | 2006-12-15 | 2007-06-27 | 湖北友芝友生物科技有限公司 | Crypthecodinium connii fermenting process for producing docosahexaenoic acid grease |
| CN101400359A (en) * | 2006-03-17 | 2009-04-01 | 草药科学新加坡私人有限公司 | Extracts and methods comprising curcuma species |
| CN102433215A (en) * | 2011-09-22 | 2012-05-02 | 厦门汇盛生物有限公司 | Method of extracting grease from fungi or algae through physical wall-breaking |
-
2015
- 2015-11-24 CN CN201510824311.4A patent/CN105385715A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1284277A (en) * | 1999-07-19 | 2001-02-21 | 大城清利 | Edible oil Radix Cureumae and its making method |
| CN101400359A (en) * | 2006-03-17 | 2009-04-01 | 草药科学新加坡私人有限公司 | Extracts and methods comprising curcuma species |
| CN1986822A (en) * | 2006-12-15 | 2007-06-27 | 湖北友芝友生物科技有限公司 | Crypthecodinium connii fermenting process for producing docosahexaenoic acid grease |
| CN102433215A (en) * | 2011-09-22 | 2012-05-02 | 厦门汇盛生物有限公司 | Method of extracting grease from fungi or algae through physical wall-breaking |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112662712A (en) * | 2021-01-26 | 2021-04-16 | 湖北福星生物科技有限公司 | Microbial-derived DHA grease, preparation method thereof and functional food |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| NL2026990B1 (en) | Microbial oil containing dha at sn-2 position and preparation method and uses therefor | |
| CN109777607B (en) | Method for purifying DHA (docosahexaenoic acid) crude oil | |
| Peng et al. | A temperature-shift strategy for efficient arachidonic acid fermentation by Mortierella alpina in batch culture | |
| CN105567752A (en) | Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors | |
| CN101631870A (en) | Method for production of polyunsaturated fatty acid and lipid containing the polyunsaturated fatty acid | |
| US11345943B2 (en) | Semi-continuous culture methods | |
| CN1986822A (en) | Crypthecodinium connii fermenting process for producing docosahexaenoic acid grease | |
| CN102911868B (en) | A kind of microbiological culture media and cultural method | |
| CN103642857A (en) | Production method for microbial lipid fermentation by utilization of corn steep liquor enzymatic hydrolysate | |
| US11466297B2 (en) | Methods of oil production in microorganisms | |
| CN1737109B (en) | Method for polyunsaturated fatty acid production using novel cell preservation technique | |
| US20240060112A1 (en) | Repeated fed-batch culture methods | |
| CN103627640A (en) | Low-chlorine medium used for culturing Schizochytrium limacinum and production method for docosahexenoic acid (DHA) by using same | |
| CN105400836A (en) | Production method for increasing unsaturated fatty acid content | |
| CN105385715A (en) | Method improving antioxidation of unsaturated fatty acid | |
| CN105331670B (en) | Split the method for pot algae Yu the hidden dinoflagellate mixed fermentation of Kou Shi | |
| CN107604009A (en) | A kind of technique of biofermentation production lycopene | |
| CN104946691A (en) | Method for producing DHA-containing oil through fermentation by utilizing schizochytrium limacinum | |
| CN107177640A (en) | A kind of method of promotion schizochytrium limacinum Lipid-producing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160309 |