CN107604009A - A kind of technique of biofermentation production lycopene - Google Patents
A kind of technique of biofermentation production lycopene Download PDFInfo
- Publication number
- CN107604009A CN107604009A CN201710981389.6A CN201710981389A CN107604009A CN 107604009 A CN107604009 A CN 107604009A CN 201710981389 A CN201710981389 A CN 201710981389A CN 107604009 A CN107604009 A CN 107604009A
- Authority
- CN
- China
- Prior art keywords
- technique
- lycopene
- fermentation
- spore
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a kind of technique of biofermentation production lycopene, it is related to field of biological pharmacy.The technique of the biofermentation production lycopene of the present invention includes：Positive bacterium spore and negative bacterium spore access physiological saline are obtained into positive bacterium and negative bacterium spore suspension respectively；It is 2 according to positive bacterium and negative bacterium spore suspension concentration ratio：35 access seed culture medium cultures obtain seed liquor；Seed liquor is accessed into fermentation medium fermented and cultured, after fermented and cultured carries out 12h, the fermentation medium of supplement and original fermentation medium equivalent into zymotic fluid.The form of the process modification seed liquor, positive and negative spore suspension is directly seeded to culture in seed culture medium and carries out conjugation, caused thalline is minimum bacterium ball or mycelia, does not produce agglomerating thalline, is advantageous to the absorption of oxygen and nutriment in its liquid medium within；Fermentation condition is improved, strengthens metabolism and the product expression activity of thalline, beneficial to the accumulation of lycopene, improves the yield of lycopene.
Description
Technical field
The present invention relates to field of biological pharmacy, especially a kind of technique of biofermentation production lycopene.
Background technology
Lycopene is a Carotenoids, has and improves body immunity, anticancer, anti-oxidant, reducing blood lipid, protection skin
Skin and other effects, it is widely used in food, medicine and cosmetic field.Natural lycopene, is primarily present in plant of Solanaceae
In the ripening fruits of tomato.As people increasingly focus on green, health, lycopene is constantly by the extensive pass of people
Note.Mankind itself can not synthesize lycopene, need to be supplemented by modes such as meals.According to U.S. CMR（Customer account management relation）It is international
Company predicts that year is increased 35% by lycopene product sale, it is contemplated that in two or three years reaches 20,000,000,000 dollars of sales volume, the world is transnational
Medical giant and domestic medical giants march lycopene industry one after another.
At present, the production method of lycopene mainly has natural extraction, chemical synthesis, microbial fermentation.It is it has proven convenient that natural
Lycopene is more easy to absorb than the lycopene of chemical synthesis, moreover, the presence of chemical synthesis chemicals residual, therefore, in recent years
To have shifted towards the exploitation of natural prodcuts.Natural lycopene is extracted from plant material and is vulnerable to condition and yield limitation, and it is micro-
Biological fermentation process produces natural lycopene and considers to be superior to above two from factors such as quality, technology, resource, cost and environment
Method, thus more application prospect.It is disadvantageous in that microorganism not currently with Production by Microorganism Fermentation lycopene
Can high-caliber accumulation lycopene, cause that fermentation production rate is low, production cost is higher.
The content of the invention
The goal of the invention of the present invention is：For above-mentioned problem, there is provided a kind of fermenting and producing side of lycopene
Method, this method have higher expression activity by improving the seed preparation condition of microorganism, the microorganism for being used in fermentation；Together
Shi Gaijin fermentation culture conditions, the metabolic fluxes of lycopene are accumulated with enhancing, so as to improve the yield of lycopene.
The technical solution adopted by the present invention is as follows：
A kind of technique of biofermentation production lycopene, it includes：
Step 1：Prepared by seed, respectively access positive bacterium spore and negative bacterium spore in sterilized physiological saline, and stirring obtains just
Bacterium spore suspension and negative bacterium spore suspension；
Step 2：Seed culture, according to concentration ratio it is 2 according to positive bacterium spore suspension and negative bacterium spore suspension：3-5 access seed trainings
Support base progress seed culture and obtain seed liquor；
Step 3：Fermented and cultured, seed liquor access fermentation medium is subjected to fermented and cultured, after fermented and cultured carries out 12h, Xiang Fa
Afterfermentation and the fresh fermentation medium of original culture medium equivalent in zymotic fluid.
The strain used in the present invention is trispore Bruce mould（Blakeslea trispora）'s（+）（-）Bacterial strain, it is former
Beginning strain is purchased from ATCC, and numbering is respectively ATCC 14271（+）, ATCC 14272（-）.It should be noted that in the present invention
Method other most of subspecies of trispore Bruce mould are also suitable.The strain without mutagenesis is directly used in the present invention,
Reason is that the strain stability through mutagenesis is not high, is not suitable for industry's enlarging production.
Connect by adopting the above-described technical solution, positive and negative spore suspension is directly seeded into culture in seed culture medium
Symphysis is grown, and caused thalline is minimum bacterium ball or mycelia, and agglomerating thalline does not occur, is advantageous to oxygen in its liquid medium within
With the absorption of nutriment.Negative bacterium is the main producing strains of lycopene, and positive bacterium produces as pairing bacterium and negative bacterium co-fermentation
Trisporic acid, so as to promote the synthesis of lycopene.Because trispore Bruce mould needs to carry out conjugation during the fermentation
Ability expression product, to improve the yield of lycopene, needs to meet certain ratio between positive bacterium and negative bacterium, properly increases negative
The ratio of bacterium, be advantageous to lycopene accumulation, but its ratio is too high, trisporic acid growing amount very little, influences Product formation.
In fermentation process, nutriment constantly consumption and the increase of concomitant fermentations fluid viscosity, supplement the nutrients substance advantageous in time
Reproduction and product expression in thalline, while reduce fermentation broth viscosity.
In preferred embodiments of the present invention, the feed postition of the fresh culture is：Respectively the 12h in fermentation,
16h, 20h, 24h are according to 1：2 ：4：8 volume ratio adds by several times.
Due to taking above-mentioned technical proposal, fresh culture adds zymotic fluid in a manner of class index, composite thallus
Growth rhythm, advantageously in the growth of thalline.
In preferred embodiments of the present invention, the positive bacterium spore and negative bacterium spore obtain by following culture：By strain
Setting-out accesses the plate of the culture medium containing potato, in sterile culture case, 26-30 DEG C of keeping temperature, cultivates 2-4d；Then reduce temperature
Degree stimulates spore to generate, and cultivate spore 1-3d to 16-20 DEG C.
By adopting the above-described technical solution, the 26-30 DEG C of optimum temperature for growth, grows 2- at this temperature
4d, strain are in the reduction of exponential phase, now cultivation temperature, are advantageous to stimulate the generation of spore.
In preferred embodiments of the present invention, during cultivating spore, oxygen is passed through into incubator with 2L/min speed,
Give illumination, intensity of illumination 4WLx simultaneously.
By adopting the above-described technical solution, the generation of the change of increase environment, further stimulation spore, on the other hand,
The thermophilic oxygen of trispore Bruce mould spore, light, is advantageous to spore growth.
In preferred embodiments of the present invention, the temperature of seed culture process is 26-30 DEG C, incubation time 1-3d, culture
Process stir speed (S.S.) is 200r/min, to the ventilation rate for giving 1vvm simultaneously, and 4WLx intensities of illumination.
By adopting the above-described technical solution, the positive and negative rapid conjugation of spore, produces spherical or thread thalline, does not send out
Generation group phenomenon.
In preferred embodiments of the present invention, it is characterised in that the composition of the fermentation medium is cornstarch 4%, grape
Sugar 2%, soybean cake powder 1%, soybean protein isolate 3%, corn steep liquor 2%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.02%, vegetable oil 2%,
VB10.5%, 2,6- di-tert-butyl-4-methy phenols 0.05%, and pH is adjusted to 7.3.
By adopting the above-described technical solution, cornstarch, glucose are carbon source, soybean cake powder, soybean protein isolate with
And corn steep liquor is nitrogen source, magnesium sulfate, vegetable oil, VB1For nourishing additive agent, 2,6- di-tert-butyl-4-methy phenols are anti-oxidant
Agent, potassium dihydrogen phosphate are stabilizer.The starting pH7.3 of zymotic fluid is advantageous to the accumulation of lycopene, but during the fermentation, pH
Need not constant control, no silhouette rings accumulation of the thalline using organic acid and lycopene caused by fermentation.
In preferred embodiments of the present invention, when spore suspension is accessed into fermentation medium, bacterium spore suspension and fermentation are born
The volume ratio of culture medium is 1：4-6.
Due to taking above-mentioned technical proposal, inoculative proportion is improved in right amount, is advantageous to increase and the product of thalline quantity
Accumulation, but inoculum concentration is too high, easily causes the serious bacteriolysis of deuterogenesis of fermenting.
In preferred embodiments of the present invention, fermented and cultured process lucifuge, incubation time 120h, cultivation temperature 22-26
DEG C, stir speed (S.S.) 400r/min, ventilation rate 1vvm.
The optimum temperature for producing lycopene is 22-26 DEG C, and temperature is too low, and microbial activity reduces, and temperature is too high will increase
The content of beta carotene and γ carrotene.The appropriate rotating speed that improves can improve dissolved oxygen speed, but high speed of agitator is formed and done
Shearing force, thalline is damaged, reduces bioactivity.
In preferred embodiments of the present invention, after fermentation carries out 48h, conditioning agent, the conditioning agent bag are added into zymotic fluid
Include leucine in mass：Abscisic acid：Creatinine：Penicillin=60：5：4：1, wherein concentration of the penicillin in zymotic fluid is
0.1g/L。
Due to taking above-mentioned technical proposal, creatinine is lycopene cox-2 inhibitors, can effectively suppress tomato red
The epoxidation of element and follow-up metabolic process, to accumulate lycopene.Abscisic acid is trisporic acid analogue, and trisporic acid is
The sex hormone of trispore Bruce mould, energy effective stimulus promote the generation of lycopene.Leucine is 3- hydroxy-3-methyl coenzyme
A precursor substance, 3- hydroxy-3-methyls coacetylase can form mevalonic acid, and penicillin is mevalonate kinase activator, activation
Mevalonate kinase, the synthesis of carotenoid precursors material Isoprenoid is improved, so as to improve yield of lycopene.Four
Kind material passes through different approaches：Or suppress lycopene and decompose, or promote its synthesis, act synergistically, to improve lycopene production
Amount.
In preferred embodiments of the present invention, after fermentation carries out 48h, glucose 1.0%, ammonium sulfate are added into zymotic fluid
1.0%, dipotassium hydrogen phosphate 0.1%.
By adopting the above-described technical solution, further supplementing bacterial metabolism needed nutrient matter, and maintain zymotic fluid
In acidity, promote lycopene accumulation.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
1. improving the form of seed liquor, positive and negative spore suspension is directly seeded into culture in seed culture medium carries out conjugation,
Caused thalline is minimum bacterium ball or mycelia, and agglomerating thalline does not occur, is advantageous to oxygen and nutrition in its liquid medium within
The absorption of material.
2. improving fermentation condition, strengthen metabolism and the product expression activity of thalline, beneficial to the accumulation of lycopene, improve
The yield of lycopene.
Embodiment
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive
Feature and/or step beyond, can combine in any way.
This specification（Including any accessory claim, summary）Disclosed in any feature, unless specifically stated otherwise,
Replaced by other equivalent or with similar purpose alternative features.I.e., unless specifically stated otherwise, each feature is a series of
An example in equivalent or similar characteristics.
Embodiment 1
The present embodiment provides a kind of technique of biofermentation production lycopene, and it comprises the following steps：
Step 1：Peeled potatoes 200g is taken, 1000mL deionized waters is added and boils and keep 30min；Two layers of gauze mistake after cooling
Filter, takes filtrate, adds deionized water to 1000mL, and add 20g glucose and 10g agar；Sterilize 30min at 115 DEG C, is made
Potato culture medium（PDA）.Take ATCC 14271（+）（Hereinafter referred to as positive bacterium）With ATCC 14272（-）（Hereinafter referred to as negative bacterium）Point
It is not coated on PDA plate, is cultivated 4 days in 26 DEG C of constant incubators, then reduces temperature to 16 DEG C, stimulate spore to generate,
And oxygen is passed through into incubator with 2L/min speed, while give the illumination cultivation spore 3d that intensity is 4WLx.
Step 2：Positive bacterium spore and negative bacterium spore are respectively connected to the triangular flask equipped with 20mL sterile salines, magnetic force
30min is stirred, obtains positive bacterium spore suspension and negative bacterium spore suspension.
Step 3：By positive bacterium spore suspension and the same triangle equipped with sterilizing seed culture medium of negative bacterium spore suspension access
Bottle, and positive the ratio between bacterium spore and negative bacterium spore concentration are 2：3, it is then 26 DEG C, stir speed (S.S.) 200r/min in temperature, ventilation
Rate is 1vvm, and cultivating 3d under conditions of intensity of illumination 4WLx obtains seed liquor.The formula of seed culture medium is（In mass）：Form sediment
Powder 4%, glucose 2.5%, corn steep liquor 5%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.01%, vitamin B1 0.001%, pH7.0 is adjusted,
It is dissolved to 1L.
Step 4：According to seed liquor：Fermentation medium=1：Seed liquor is seeded to sterilized fermented and cultured by 4 ratio
In base, the volume of original fermentation medium is calculated as V0, the formula of fermentation medium is（In mass）：Cornstarch 4%, Portugal
Grape sugar 2%, soybean cake powder 1%, soybean protein isolate 3%, corn steep liquor 2%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.02%, vegetable oil
2%, vitamin B10.5%, 2,6- di-tert-butyl-4-methy phenols 0.05%, and pH is adjusted to 7.3.
Fermented and cultured continues 120h, and temperature is 22 DEG C, stir speed (S.S.) 400r/min, ventilation rate 1vvm.Fermentation is carried out
After 12h, it is V to supplement volume into zymotic fluid0Fresh culture.Preferably, fresh culture respectively the 12h in fermentation,
16h, 20h, 24h are according to 1：2 ：4：8 volume ratio adds by several times.After fermentation carries out 48h, conditioning agent is added into zymotic fluid, with
Adjust metabolic fluxes.The conditioning agent includes leucine in mass：Abscisic acid：Creatinine：Penicillin=60：5：4：1, wherein blue or green
Concentration of the mycin in zymotic fluid is 0.1g/L.While adding conditioning agent, glucose 1.0%, ammonium sulfate are added into zymotic fluid
1.0%, dipotassium hydrogen phosphate 0.1% is with the material that supplements the nutrients.
Embodiment 2
The present embodiment provides a kind of technique of biofermentation production lycopene, and it comprises the following steps：
Step 1：Peeled potatoes 200g is taken, 1000mL deionized waters is added and boils and keep 30min；Two layers of gauze mistake after cooling
Filter, takes filtrate, adds deionized water to 1000mL, and add 20g glucose and 10g agar；Sterilize 30min at 115 DEG C, is made
Potato culture medium（PDA）.Take ATCC 14271（+）（Hereinafter referred to as positive bacterium）With ATCC 14272（-）（Hereinafter referred to as negative bacterium）Point
It is not coated on PDA plate, is cultivated 3 days in 28 DEG C of constant incubators, then reduces temperature to 18 DEG C, stimulate spore to generate,
And oxygen is passed through into incubator with 2L/min speed, while give the illumination cultivation spore 2d that intensity is 4WLx.
Step 2：Positive bacterium spore and negative bacterium spore are respectively connected to the triangular flask equipped with 20mL sterile salines, magnetic force
30min is stirred, obtains positive bacterium spore suspension and negative bacterium spore suspension.
Step 3：By positive bacterium spore suspension and the same triangle equipped with sterilizing seed culture medium of negative bacterium spore suspension access
Bottle, and positive the ratio between bacterium spore and negative bacterium spore concentration are 1：2, it is then 28 DEG C, stir speed (S.S.) 200r/min in temperature, ventilation
Rate is 1vvm, and cultivating 2d under conditions of intensity of illumination 4WLx obtains seed liquor.The formula of seed culture medium is（In mass）：Form sediment
Powder 4%, glucose 2.5%, corn steep liquor 5%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.01%, vitamin B1 0.001%, pH7.0 is adjusted,
It is dissolved to 1L.
Step 4：According to seed liquor：Fermentation medium=1：Seed liquor is seeded to sterilized fermented and cultured by 5 ratio
In base, the volume of original fermentation medium is calculated as V0, the formula of fermentation medium is（In mass）：Cornstarch 4%, Portugal
Grape sugar 2%, soybean cake powder 1%, soybean protein isolate 3%, corn steep liquor 2%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.02%, vegetable oil
2%, vitamin B10.5%, 2,6- di-tert-butyl-4-methy phenols 0.05%, and pH is adjusted to 7.3.
Fermented and cultured continues 120h, and temperature is 25 DEG C, stir speed (S.S.) 400r/min, ventilation rate 1vvm.Fermentation is carried out
After 12h, it is V to supplement volume into zymotic fluid0Fresh culture.Preferably, fresh culture respectively the 12h in fermentation,
16h, 20h, 24h are according to 1：2 ：4：8 volume ratio adds by several times.After fermentation carries out 48h, conditioning agent is added into zymotic fluid, with
Adjust metabolic fluxes.The conditioning agent includes leucine in mass：Abscisic acid：Creatinine：Penicillin=60：5：4：1, wherein blue or green
Concentration of the mycin in zymotic fluid is 0.1g/L.While adding conditioning agent, glucose 1.0%, ammonium sulfate are added into zymotic fluid
1.0%, dipotassium hydrogen phosphate 0.1% is with the material that supplements the nutrients.
Embodiment 3
The present embodiment provides a kind of technique of biofermentation production lycopene, and it comprises the following steps：
Step 1：Peeled potatoes 200g is taken, 1000mL deionized waters is added and boils and keep 30min；Two layers of gauze mistake after cooling
Filter, takes filtrate, adds deionized water to 1000mL, and add 20g glucose and 10g agar；Sterilize 30min at 115 DEG C, is made
Potato culture medium（PDA）.Take ATCC 14271（+）（Hereinafter referred to as positive bacterium）With ATCC 14272（-）（Hereinafter referred to as negative bacterium）Point
It is not coated on PDA plate, is cultivated 2 days in 30 DEG C of constant incubators, then reduces temperature to 20 DEG C, stimulate spore to generate,
And oxygen is passed through into incubator with 2L/min speed, while give the illumination cultivation spore 1d that intensity is 4WLx.
Step 2：Positive bacterium spore and negative bacterium spore are respectively connected to the triangular flask equipped with 20mL sterile salines, magnetic force
30min is stirred, obtains positive bacterium spore suspension and negative bacterium spore suspension.
Step 3：By positive bacterium spore suspension and the same triangle equipped with sterilizing seed culture medium of negative bacterium spore suspension access
Bottle, and positive the ratio between bacterium spore and negative bacterium spore concentration are 2：5, it is then 30 DEG C, stir speed (S.S.) 200r/min in temperature, ventilation
Rate is 1vvm, and cultivating 1d under conditions of intensity of illumination 4WLx obtains seed liquor.The formula of seed culture medium is（In mass）：Form sediment
Powder 4%, glucose 2.5%, corn steep liquor 5%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.01%, vitamin B1 0.001%, pH7.0 is adjusted,
It is dissolved to 1L.
Step 4：According to seed liquor：Fermentation medium=1：Seed liquor is seeded to sterilized fermented and cultured by 6 ratio
In base, the volume of original fermentation medium is calculated as V0, the formula of fermentation medium is（In mass）：Cornstarch 4%, Portugal
Grape sugar 2%, soybean cake powder 1%, soybean protein isolate 3%, corn steep liquor 2%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.02%, vegetable oil
2%, vitamin B10.5%, 2,6- di-tert-butyl-4-methy phenols 0.05%, and pH is adjusted to 7.3.
Fermented and cultured continues 120h, and temperature is 26 DEG C, stir speed (S.S.) 400r/min, ventilation rate 1vvm.Fermentation is carried out
After 12h, it is V to supplement volume into zymotic fluid0Fresh culture.Preferably, fresh culture respectively the 12h in fermentation,
16h, 20h, 24h are according to 1：2 ：4：8 volume ratio adds by several times.After fermentation carries out 48h, conditioning agent is added into zymotic fluid, with
Adjust metabolic fluxes.The conditioning agent includes leucine in mass：Abscisic acid：Creatinine：Penicillin=60：5：4：1, wherein blue or green
Concentration of the mycin in zymotic fluid is 0.1g/L.While adding conditioning agent, glucose 1.0%, ammonium sulfate are added into zymotic fluid
1.0%, dipotassium hydrogen phosphate 0.1% is with the material that supplements the nutrients.
The invention is not limited in foregoing embodiment.The present invention, which expands to, any in this manual to be disclosed
New feature or any new combination, and disclose any new method or process the step of or any new combination.
Claims (10)
1. a kind of technique of biofermentation production lycopene, it is characterised in that it includes：
Step 1：Prepared by seed, respectively access positive bacterium spore and negative bacterium spore in sterilized physiological saline, and stirring obtains just
Bacterium spore suspension and negative bacterium spore suspension；
Step 2：Seed culture, it is 2 according to positive bacterium spore suspension and negative bacterium spore suspension concentration ratio：3-5 accesses seed culture medium
Carry out seed culture and obtain seed liquor；
Step 3：Fermented and cultured, seed liquor access fermentation medium is subjected to fermented and cultured, after fermented and cultured carries out 12h, Xiang Fa
The fresh fermentation medium of supplement and original fermentation medium equivalent in zymotic fluid.
2. the technique of biofermentation production lycopene according to claim 1, it is characterised in that the fresh culture
Feed postition be：Respectively fermentation 12h, 16h, 20h, 24h according to 1：2 ：4：8 volume ratio adds by several times.
3. the technique of biofermentation according to claim 2 production lycopene, it is characterised in that the positive bacterium spore and
Negative bacterium spore obtains by following culture：By the plate of strain setting-out access culture medium containing potato, in sterile culture case, protect
26-30 DEG C of temperature is held, cultivates 2-4d；Temperature is then reduced to 16-20 DEG C, stimulates spore to generate, and cultivate spore 1-3d.
4. the technique of biofermentation production lycopene according to claim 3, it is characterised in that culture spore process
In, oxygen is passed through into incubator with 2L/min speed, while illumination is given, intensity of illumination 4WLx.
5. the technique of biofermentation production lycopene according to claim 4, it is characterised in that seed culture process
Temperature is 26-30 DEG C, incubation time 1-3d, and incubation stir speed (S.S.) be 200r/min, to and meanwhile give 1vvm ventilation rate,
And 4WLx intensities of illumination.
6. the technique of biofermentation production lycopene according to claim 5, it is characterised in that the fermentation medium
Composition be cornstarch 4%, glucose 2%, soybean cake powder 1%, soybean protein isolate 3%, corn steep liquor 2%, potassium dihydrogen phosphate
0.1%, magnesium sulfate 0.02%, vegetable oil 2%, VB10.5%, 2,6- di-tert-butyl-4-methy phenols 0.05%, and pH is adjusted to 7.3.
7. the technique of the biofermentation production lycopene according to any one of claim 1-6, it is characterised in that Xiang Fa
When spore suspension is accessed in ferment culture medium, the volume ratio for bearing bacterium spore suspension and fermentation medium is 1：4-6.
8. the technique of biofermentation production lycopene according to claim 7, it is characterised in that fermented and cultured process is kept away
Light, incubation time 120h, cultivation temperature are 22-26 DEG C, stir speed (S.S.) 400r/min, ventilation rate 1vvm.
9. the technique of biofermentation production lycopene according to claim 9, it is characterised in that after fermentation carries out 48h,
Conditioning agent is added into zymotic fluid, the conditioning agent includes leucine in mass：Abscisic acid：Creatinine：Penicillin=60：5：
4：1, wherein concentration of the penicillin in zymotic fluid is 0.1g/L.
10. the technique of biofermentation production lycopene according to claim 9, it is characterised in that fermentation carries out 48h
Afterwards, glucose 1.0%, ammonium sulfate 1.0%, dipotassium hydrogen phosphate 0.1% are added into zymotic fluid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710981389.6A CN107604009A (en) | 2017-10-20 | 2017-10-20 | A kind of technique of biofermentation production lycopene |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710981389.6A CN107604009A (en) | 2017-10-20 | 2017-10-20 | A kind of technique of biofermentation production lycopene |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107604009A true CN107604009A (en) | 2018-01-19 |
Family
ID=61078923
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710981389.6A Withdrawn CN107604009A (en) | 2017-10-20 | 2017-10-20 | A kind of technique of biofermentation production lycopene |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107604009A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108277238A (en) * | 2018-04-09 | 2018-07-13 | 中国科学院合肥物质科学研究院 | A kind of method and lycopene of Blakeslea trispora fermentation production lycopene |
-
2017
- 2017-10-20 CN CN201710981389.6A patent/CN107604009A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108277238A (en) * | 2018-04-09 | 2018-07-13 | 中国科学院合肥物质科学研究院 | A kind of method and lycopene of Blakeslea trispora fermentation production lycopene |
| CN108277238B (en) * | 2018-04-09 | 2021-09-21 | 中国科学院合肥物质科学研究院 | Method for producing lycopene by fermenting blakeslea trispora and lycopene |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102168115A (en) | Industrialized production method of coenzyme Q10 | |
| CN103173371B (en) | Production of saccharomyces cerevisiae and lactobacillus acidophilus composite microbe preparation used for feed | |
| CN105506048B (en) | A kind of fermentation process preparing beta carotene using Blakeslea trispora | |
| CN103250568A (en) | Method for preparing hericium erinaceus rich in selenium | |
| CN103740772B (en) | A kind of method promoting camphor tree sesame liquid state fermentation biosynthesizing Android tonquinol | |
| CN106148451B (en) | Biological cellulose gel culture medium and the biological cellulose gel product prepared with it | |
| CN102381896B (en) | Crab-flavor mushroom liquid strain medium formula and preparation method thereof | |
| CN107868778A (en) | A kind of preparation method of Se-enriched yeast | |
| CN110982750A (en) | High-density fermentation method for rhodopseudomonas palustris and application of high-density fermentation method | |
| CN108719991B (en) | A method for preparing red rice containing Bacillus natto from bean dregs and bean curd yellow serofluid | |
| CN102225875B (en) | Medium and cultivating method for Tibetan mushroom rapid propagation | |
| CN109182397A (en) | A method of improving reuterin yield | |
| CN108034687A (en) | A kind of preparation method of composition containing carotenoid and the composition containing carotenoid | |
| CN107604009A (en) | A kind of technique of biofermentation production lycopene | |
| CN108018324A (en) | A kind of fermentation medium for producing doractin and preparation method and application | |
| CN103497896B (en) | A kind of dehydration pit mud functional bacteria protective material and application thereof | |
| CN107604010A (en) | A kind of fermentation manufacturing technique of lycopene | |
| CN110004068A (en) | A kind of mushroom strain storage medium and method for preserving | |
| CN116120110A (en) | Application of rhodozyma in microbial compound bacterial fertilizer and rhodozyma compound fertilizer | |
| CN101861796B (en) | Method for culturing amanita pantherina by using waste distillage after fermentation of coloured rice | |
| CN101845475B (en) | Nutrition-enhanced culture medium for preparing 2-KGA through fermentation and method thereof for preparing 2-KGA | |
| CN103387427B (en) | Method for manufacture of biofertilizer by pleuromutilin bacterial residue harmless treatment | |
| CN103103062A (en) | Method for preparing nutritional mulberry beverage from composite probiotics | |
| CN104711208B (en) | A kind of lactic acid bacteria with high starch capacity of decomposition | |
| CN108517338B (en) | Method for producing arachidonic acid oil by fermenting mortierella alpina based on active oxygen regulation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180119 |