CN102430046A - Aquatic product alive-keeping agent and preparation method and application thereof - Google Patents
Aquatic product alive-keeping agent and preparation method and application thereof Download PDFInfo
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- CN102430046A CN102430046A CN2011102532818A CN201110253281A CN102430046A CN 102430046 A CN102430046 A CN 102430046A CN 2011102532818 A CN2011102532818 A CN 2011102532818A CN 201110253281 A CN201110253281 A CN 201110253281A CN 102430046 A CN102430046 A CN 102430046A
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Abstract
The invention discloses the application of the following compositions in part by weight to the preparation of an aquatic product alive-keeping agent: 225 to 375 parts of codonopsis pilosula, 150 to 250 parts of dwarf lilyturf root and 75 to 125 parts of schisandra chinensis. The invention also provides the aquatic product alive-keeping agent, a preparation method thereof and the application thereof. The aquatic product alive-keeping agent is prepared from pure traditional Chinese medicines, can effectively improve the survival rate of various aquatic products during living body transportation, and has the advantages of safety, high efficiency and the like. The invention provides a new choice for the living body transportation of aquatic products.
Description
Technical field
The present invention relates to a kind of natural aquatic product keep-alive agent, and the method for preparing of this live keeping agent and purposes.
Background technology
Aquatic products are one type and take the biology of factor as the leading factor with water, mainly are divided into four types of fishes, shrimp, Eriocheir sinensis, shellfish, are one big type of indispensable in life diet sources.Along with improving constantly of living standard, people are also increasingly high to the prescription of aquatic products, all hope to buy aquatic products alive, and this also just makes this mode of the live transport important that seems.
In the live transport process,, will avoid the death of live body aquatic products and reduce the weak loss that is brought of the fish asthenia that causes owing to poor environment for quality and the economic worth that guarantees aquatic products.At present, water keep-alive transportation technology being arranged is one of live transport technology of using always.In water keep-alive transportation was arranged, the key factor that influences the variation of live body physiological situation had 1) oxygen: anoxia can cause its death by suffocation.2) the various aquatic products of temperature all have the comfort zone of oneself, and it is just dead easily to exceed comfort zone.3) carbon dioxide: carbon dioxide causes death by suffocation to aquatic products.4) ammonia nitrogen: ammonia nitrogen runs up to finite concentration, and aquatic products can be poisoned deadly.5) osmotic pressure of organism: in the transportation, the body surface regular meeting epidermis galling of fish, shrimp etc. makes unbalance the causing death of the interior osmotic pressure of body.6) body constitution of organism: the body constitution of organism is related to survival rate.7) breeding of antibacterial: a large amount of breedings rapidly of pathogenic bacteria are prone to make aquatic products dead.
In order to prolong the time-to-live of aquatic products when the live transport; The metabolism that adopt means such as low temperature, anesthesia to reduce aquatic products more; Reduce the oxygen consumption and the Excreta of aquatic products; Guarantee water quality, also can reduce the consumption of aquatic products endotrophic material simultaneously to the utmost, thereby improve the aquatic products survival rate.Like MS-222, be Aquatic product anesthetis commonly used, be the safest, the most reliable anaesthetic of countries in the world extensive use; Accumulate but it has at organs such as spleen, livers, adopt this narcotic aquatic products, need in clear water, to raise after 21 days and could sell; And this anesthetis is after the sunlight direct projection, to fish have strong toxicity (Liu Changlin, etc.; Fish anesthesia Review Study, fishery modernization, 2007 34 5 phases of volume).At present, research worker is arranged,, also used other additives such as antibacterial, buffer agent adding on the narcotic basis in order further to improve the survival rate of aquatic products.Like application number: CN92115229.9, that the live keeping agent in this application has adopted is stable, atropine sulfate, gentamycin, phosphate buffer and hydrogen peroxide, and its keep-alive time is long.But a stable type medicine has addiction property, and antibiotic life-time service also can produce drug resistance.To sum up, in existing Aquatic product live keeping agent, because the existence of chemical anesthetis, antibiotic etc makes the aquatic products that transported have potential chemical drugs harm.
The Chinese medicine kind is numerous, and is little to the toxic and side effects of human body, in Chinese medicine, seeks natural aquatic product keep-alive agent, becomes important research direction in this field.Be natural medicinal ingredients like eugenol, cheap and easy to get, health there is not influence, have safety preferably, be a kind of anesthetis of being paid close attention at present.SHENGMAI YIN is formed QI invigorating by Radix Ginseng (Radix Codonopsis side is claimed in Radix Codonopsis replacement back), Radix Ophiopogonis, Fructus Schisandrae Chinensis three flavor medical materials; YIN nourishing and the production of body fluid promoting is used for QIYINLIANGXU, shortness of breath and palpitation; Spontaneous perspiration is through experiment showed, SHENGMAI YIN non-toxic reaction (Ma Liang etc.; The rat long term toxicity research of SHENGMAI YIN, Zhejiang preventive medicine, 2003 15 3 phases of volume); Borneolum Syntheticum, the refreshment of having one's ideas straightened out, clearing away heat to alleviate pain is used for calentura coma, convulsions, the apoplexy syncope due to accumulation of phlegm; Stagnation of QI sudden syncope, attacked by pestiferous factors stupor, obstruction of qi in the chest and cardialgia, conjunctival congestion, aphtha; Laryngopharynx swelling and pain, auditory meatus is suppurated, and research shows that Borneolum Syntheticum does not have obvious toxic-side effects; Only when heavy dose is used, centering, late period, the anemia of pregnant woman had induced labor effect (Tianjin Chinese medicine, 2005 22 5 phases of volume, 371-374 page or leaf; Chinese patent medicine research, 1986,5 (3): 1).
At present, do not see the relevant report that SHENGMAI YIN, Borneolum Syntheticum and SHENGMAI YIN compatibility is used for the aquatic product keep-alive agent both at home and abroad as yet.
Summary of the invention
The purpose of technical scheme of the present invention provides the new purposes of SHENGMAI YIN; Another purpose of technical scheme of the present invention provides a kind of aquatic product keep-alive agent, and the method for preparing of this live keeping agent and purposes; The present invention also provides a kind of keepalive method of aquatic products.The aquatic product keep-alive agent is one type of additive in the life water body of aquatic products, uses anesthetis, tranquilizer, antibacterial, oxygenate, anti-damage agent etc. usually, can be used for improving the survival rate of aquatic products.
The invention provides the purposes of compositions in the agent of preparation aquatic product keep-alive of following weight proportioning:
Radix Codonopsis 225-375 part, Radix Ophiopogonis 150-250 part, Fructus Schisandrae Chinensis 75-125 part.
Further, the weight proportion of said compositions is following:
300 parts of Radix Codonopsis, 200 parts of Radix Ophiopogonis, 100 parts of Fructus Schisandrae Chinensis.
The present invention also provides a kind of aquatic product keep-alive agent, and it is to be prepared from following materials of weight proportions medicine:
Borneolum Syntheticum 3.6-6 part, Radix Codonopsis 225-375 part, Radix Ophiopogonis 150-250 part, Fructus Schisandrae Chinensis 75-125 part.
Further, it is to be prepared from following materials of weight proportions medicine:
4.8 parts of Borneolum Syntheticums, Radix Codonopsis 300-375 part, Radix Ophiopogonis 200-250 part, Fructus Schisandrae Chinensis 100-125 part.
Further, it is to be prepared from following materials of weight proportions medicine:
4.8 parts of Borneolum Syntheticums, Radix Codonopsis, Radix Ophiopogonis and Fructus Schisandrae Chinensis total amount are 600-750 part, and wherein, Radix Codonopsis: Radix Ophiopogonis: Fructus Schisandrae Chinensis is 300: 200: 100W/W.
Further preferably, it is to be prepared from following materials of weight proportions medicine:
4.8 parts of Borneolum Syntheticums, 300 parts of Radix Codonopsis, 200 parts of Radix Ophiopogonis, 100 parts of Fructus Schisandrae Chinensis.
Further, it is to be active component by the water of Radix Codonopsis, Radix Ophiopogonis, Fructus Schisandrae Chinensis or extractive with organic solvent, adds the preparation that acceptable other adjuvant is prepared from Borneolum Syntheticum and the aquatic product keep-alive agent, and wherein, described adjuvant is emulsifying agent, ethanol, water; Its consumption is following: Borneolum Syntheticum: emulsifying agent is 1: 2-3W/V, Borneolum Syntheticum: ethanol is 1: 10-20W/V, water is an amount of.
Wherein, said ethanol is 95%V/V ethanol.
Wherein, emulsifying agent is Polysorbate, polyoxyethylene nonylphenol ether, poloxamer, arabic gum, gelatin, phospholipid, tragacanth, Rhizoma Bletillae gel; Preferred Polysorbate.
Further, described emulsifying agent is a Tween-80, Borneolum Syntheticum: Tween-80 is 1: 2.5W/V.
Further preferably, every 1200ml Aquatic product live keeping agent is by Borneolum Syntheticum 4.8g, and SHENGMAI YIN compound recipe 600-750g is prepared from, and wherein, SHENGMAI YIN compound recipe proportioning is 300 parts of Radix Codonopsis, 200 parts of Radix Ophiopogonis, 100 parts of Fructus Schisandrae Chinensis.
Further preferably, every 1200ml Aquatic product live keeping agent is by Borneolum Syntheticum 4.8g, and SHENGMAI YIN compound recipe 600g is prepared from, and wherein, SHENGMAI YIN compound recipe proportioning is 300 parts of Radix Codonopsis, 200 parts of Radix Ophiopogonis, 100 parts of Fructus Schisandrae Chinensis.
The present invention also provides a kind of method for preparing above-mentioned live keeping agent, and it has following operating procedure:
A, by the prescription proportioning get Borneolum Syntheticum, add the emulsifying agent mixing after, add ethanol again, the dissolving, the Borneolum Syntheticum alcoholic solution subsequent use;
B, by the prescription proportioning get Radix Codonopsis, Radix Ophiopogonis, Fructus Schisandrae Chinensis, after the decocte with water, condensed water decocting liquid, precipitate with ethanol; Filter, after filtrate recycling ethanol also concentrates, add the Borneolum Syntheticum alcoholic solution of A step preparation again, mixing; Thin up, and regulate pH value to 5-7, filter, get filtrating and promptly get live keeping agent.
Wherein, the concrete grammar of B step is:
Get Radix Codonopsis, Radix Ophiopogonis, Fructus Schisandrae Chinensis, decocte with water 2-3 time, each 1-3 hour, merge decocting liquid, filter; After filtrating concentrates, add ethanol, leave standstill, filter to containing more than the alcohol amount 50%V/V; Decompression filtrate recycling ethanol also is concentrated into and contains crude drug 1g/ml, adds the Borneolum Syntheticum alcoholic solution that the A step obtains again, mixing, thin up; Regulate pH value to 5-7, leave standstill, filter, promptly get live keeping agent.
The present invention also provides the purposes of above-mentioned live keeping agent aspect the survival rate of raising aquatic products in transportation.
Wherein, described aquatic products are that Carassius auratus, Silurus asotus fish, Misgurni anguillicaudati, astacus, base enclose shrimp, crab, flower clam or Corbicula fluminea.
The present invention also provides a kind of keepalive method of aquatic products, and method is following: get the above-mentioned live keeping agent of effective dose, join in the life water body of aquatic products, mixing gets final product.
Wherein, said aquatic products are shrimp, shellfish aquatic products, in life water body, add live keeping agent, to the concentration of Borneolum Syntheticum be 10-20mg/L, preferred 20mg/L.
Wherein, said aquatic products are fish, Eriocheir sinensis class aquatic products, in life water body, add live keeping agent, to the concentration of Borneolum Syntheticum be 20-40mg/L, preferred 40mg/L.
Aquatic product keep-alive agent of the present invention is processed by natural Chinese medicines, can effectively improve the survival rate of the live transport of various aquatic products, has advantages such as safe, efficient, for the live transport of aquatic products provides a kind of new selection.
Obviously, according to foregoing of the present invention,,, can also make modification, replacement or the change of other various ways not breaking away under the above-mentioned basic fundamental thought of the present invention prerequisite according to the ordinary skill knowledge and the customary means of this area.
Below, foregoing of the present invention is remake further detailed description through the specific embodiment of embodiment form.But should this be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following instance.All technology that realizes based on foregoing of the present invention all belong to scope of the present invention.
Description of drawings
The flow chart of Fig. 1 aquatic product keep-alive agent of the present invention method for preparing.
The specific embodiment
The method for preparing of embodiment 1 aquatic product keep-alive agent of the present invention
Get Borneolum Syntheticum 4.8g, behind addition polymerization Pyrusussuriensis ester-8012ml mixing, add 48ml ethanol and make dissolving, it is subsequent use promptly to get the Borneolum Syntheticum alcoholic solution; Other gets Radix Codonopsis 300g, Radix Ophiopogonis 200g, Fructus Schisandrae Chinensis 100g, decocte with water secondary, 2 hours for the first time, 1.5 hours for the second time, collecting decoction; Filter, filtrating is concentrated into and contains crude drug 2g/ml, puts coldly, adds 2 times of volume ethanol of water extract, places 24 hours; Filter, filtrate decompression is returned ethanol and is concentrated into and contains crude drug 1g/ml, adds above-mentioned Borneolum Syntheticum alcoholic solution again, and mixing adds water to 1200ml; Regulate pH value to 5-7, leave standstill, filter, promptly get aquatic product keep-alive agent of the present invention.These article crude drug concentration is about 0.5g/ml.
The method for preparing of embodiment 2 aquatic product keep-alive agent of the present invention
Get Borneolum Syntheticum 4.8g, behind addition polymerization Pyrusussuriensis ester-8012ml mixing, add 96ml ethanol and make dissolving, it is subsequent use promptly to get the Borneolum Syntheticum alcoholic solution; Other gets Radix Codonopsis 300g, Radix Ophiopogonis 200g, Fructus Schisandrae Chinensis 100g, decocte with water secondary, 3 hours for the first time, 1 hour for the second time, collecting decoction; Filter, filtrating is concentrated into and contains crude drug 2g/ml, puts coldly, adds 2 times of volume ethanol of water extract, places 24 hours; Filter, filtrate decompression is returned ethanol and is concentrated into and contains crude drug 1g/ml, adds above-mentioned Borneolum Syntheticum alcoholic solution again, and mixing adds water to 1200ml; Regulate pH value to 5-7, leave standstill, filter, promptly get aquatic product keep-alive agent of the present invention.
The method for preparing of embodiment 3 aquatic product keep-alive agent of the present invention
Get Borneolum Syntheticum 3.8g, behind addition polymerization Pyrusussuriensis ester-809.5ml mixing, add 38ml ethanol and make dissolving, it is subsequent use promptly to get the Borneolum Syntheticum alcoholic solution; Other gets Radix Codonopsis 240g, Radix Ophiopogonis 240g, Fructus Schisandrae Chinensis 120g, decocte with water three times, and each each 1 hour, collecting decoction filtered; Filtrating is concentrated into and contains crude drug 2g/ml, puts coldly, adds 2 times of volume ethanol of water extract, places filtration 24 hours; Filtrate decompression is returned ethanol and is concentrated into and contains crude drug 1g/ml, adds above-mentioned Borneolum Syntheticum alcoholic solution again, and mixing adds water to 1200ml; Regulate pH value to 5-7, leave standstill, filter, promptly get aquatic product keep-alive agent of the present invention.
The method for preparing of embodiment 4 aquatic product keep-alive agent of the present invention
Get Borneolum Syntheticum 5.8g, behind addition polymerization Pyrusussuriensis ester-8017.4ml mixing, add 116ml ethanol and make dissolving, it is subsequent use promptly to get the Borneolum Syntheticum alcoholic solution; Other gets Radix Codonopsis 360g, Radix Ophiopogonis 160g, Fructus Schisandrae Chinensis 80g, decocte with water 4 times, 2 hours for the first time, each later on each 1 hour, collecting decoction; Filter, filtrating is concentrated into and contains crude drug 2g/ml, puts coldly, adds 2 times of volume ethanol of water extract, places 24 hours; Filter, filtrate decompression is returned ethanol and is concentrated into and contains crude drug 1g/ml, adds above-mentioned Borneolum Syntheticum alcoholic solution again, and mixing adds water to 1200ml; Regulate pH value to 5-7, leave standstill, filter, promptly get aquatic product keep-alive agent of the present invention.
The consumption screening experiment of each composition in the embodiment 5 aquatic product keep-alive agent of the present invention
1. the Borneolum Syntheticum working concentration confirms
Borneolum Syntheticum adds 2.5 times of amount Tween-80 mixings, adds 10-20 again and doubly measures dissolve with ethanol and become the dense alcoholic solution of Borneolum Syntheticum, adds water when needing and suitably dilutes.Misgurni anguillicaudati (the long 7-9cm of body), astacus (the long 5-7cm of body), Carassius auratus (the long 15-20cm of body) and crab (body weight 150-200g) are put into the Borneolum Syntheticum solution (each concentration group is put experimental animal 10 tails) that concentration is respectively 10mg/L, 20mg/L, 30mg/L, 40mg/L, 100mg/L, measure the time required required time of anesthesia with recovery.The anesthesia required time is meant that animal is placed into anesthetic solution and begins to the time of animal body rollover.Dopey animal taken out from medicinal liquid be put back in the clear water, from this time play can see animal begin with the normal attitude required time of swimming be the recovery time.The awake rate of record recovery simultaneously.The experimental data of the various aquatic animal anaesthetic effects of Borneolum Syntheticum medicinal liquid of different solubility is seen table 1-4.
The Borneolum Syntheticum medicinal liquid of the different solubility of table 1 is to the anaesthetic effect of Misgurni anguillicaudati
The Borneolum Syntheticum medicinal liquid of the different solubility of table 2 is to the anaesthetic effect of astacus
The Borneolum Syntheticum medicinal liquid of the different solubility of table 3 is to the anaesthetic effect of Carassius auratus
The Borneolum Syntheticum medicinal liquid of the different solubility of table 4 is to the anaesthetic effect of crab
It is 20mg/L to the righttest anesthetic concentration of small aquatic products such as Misgurni anguillicaudati, astacus that above experimental data draws Borneolum Syntheticum, and is 40mg/L to the righttest anaesthesia dosage of the big slightly aquatic animal of builds such as crab, Carassius auratus.
2. the SHENGMAI YIN extract concentration is confirmed
2.1 the preparation of SHENGMAI YIN extracting solution
Get Radix Codonopsis 300g, Radix Ophiopogonis 200g, Fructus Schisandrae Chinensis 100g, decocte with water secondary, 2 hours for the first time, 1.5 hours for the second time; Collecting decoction filters, and filtrating is concentrated into and contains crude drug 2g/ml; Put coldly, add 2 times of volume ethanol of water extract, placed 24 hours; Filter, filtrate decompression is returned ethanol and is concentrated into and contains crude drug 1g/ml, promptly gets the SHENGMAI YIN extracting solution.
2.2 definite test of SHENGMAI YIN extract concentration
Misgurni anguillicaudati (the long 7-9cm of body), astacus (the long 5-7cm of body), Carassius auratus (the long 15-20cm of body) and crab (body weight 150-200g) are divided into 5 groups at random, are divided into matched group and experiment 1-4 group, every group of 6-10 only.(it is the box of 250ml that Misgurni anguillicaudati and astacus are selected volume for use to the plastics box that matched group seals with band; It is the box of 1000ml that Carassius auratus and crab are selected volume for use; ) fill and boil after refrigerative tap water, put into the aquatic products of specified quantity.Test group then is respectively charged into the dilute solution of 1 ‰, 2.5 ‰, 5 ‰, 10 ‰, 20 ‰ Chinese medicine stock solution, and (volume ratio V: V), every then case is put into the aquatic products of specified quantity.Build plastic box, do not stay bubble in the case.Box is inverted in the water, and the time and the mantissa of animal dead in observation and the interpretation of records case are to inquire into the influence of each concentration Chinese medicine to the aquatic biological time-to-live.DPS software is adopted in data statistics; Data represent that with
difference adopts the T check.The SHENGMAI YIN extracting solution of variable concentrations is seen table 5-8 to the influence of four kinds of aquatic animal time-to-live.
The SHENGMAI YIN extracting solution of table 5 variable concentrations is to the influence of Misgurni anguillicaudati time-to-live
Remarks:
*P＜0.05,
*P＜0.01
The SHENGMAI YIN extracting solution of table 6 variable concentrations is to the influence of astacus time-to-live
Remarks:
*P＜0.05,
*P＜0.01
The SHENGMAI YIN extracting solution of table 7 variable concentrations is to the influence of Carassius auratus time-to-live
Remarks:
*P＜0.05,
*P＜0.01
The SHENGMAI YIN extracting solution of table 8 variable concentrations is to the influence of crab time-to-live
Remarks:
*P＜0.05,
*P＜0.01
Above data show: small aquatic products such as Misgurni anguillicaudati, astacus are that 2.5 ‰ o'clock effects are best at SHENGMAI YIN extracting solution working concentration; And to the big slightly aquatic animal of builds such as crab, Carassius auratus, SHENGMAI YIN extracting solution working concentration is that 5 ‰ o'clock effects are best.Therefore, confirm that SHENGMAI YIN extracting solution working concentration 2.5 ‰ is a basic concentration, can add consumption with one times for larger animal.
The screening of embodiment 6 keep-alive formula of liquid of the present invention
The active component of compound recipe keep-alive liquid of the present invention is Borneolum Syntheticum and SHENGMAI YIN extracting solution, and the optimum amount of single Borneolum Syntheticum and SHENGMAI YIN extract is studied through embodiment 5, writes out a prescription the optimum formula of research compound recipe keep-alive liquid on this basis below the design.
The design of table 9 keep-alive liquid different formulations
*Annotate: the preparation of SHENGMAI YIN extract is with in the instance five 2.1.
According to the prescription of table 9, get Borneolum Syntheticum, add the Tween-80 mixing of 2.5 times of amounts after, add 10 times the amount ethanol make dissolving, it is subsequent use promptly to get the Borneolum Syntheticum alcoholic solution; Other gets SHENGMAI YIN extract (pressing 2.1 preparations down among the embodiment 5), adds above-mentioned Borneolum Syntheticum alcoholic solution, and mixing adds water to 1200ml, regulates pH value to 5-7, leaves standstill, and filters the aquatic product keep-alive agent that promptly gets different formulations.
Keep-alive effect experimental technique: to toys such as Misgurni anguillicaudati, astacus, in every L life water body (25-28 ℃), add live keeping agent 5ml, put into animal behind the mixing; In every L life water body (25-28 ℃), add live keeping agent 10ml when transporting than large animal, put into animal behind the mixing for builds such as Carassius auratus, crabs.Observe the keep-alive effect of live keeping agent to aquatic products.The result sees table 10.
The screening study of table 10 keep-alive liquid of the present invention compatibility
Annotate: compare with the blank group
*P＜0.05
*P＜0.01
Experimental result shows: each prescription group and blank control group relatively all can obviously increase the time-to-live and the survival rate of aquatic products, and be better than other proportionings with the live keeping agent effect of 2,5 preparations of writing out a prescription; Wherein, 2 best results of writing out a prescription, promptly every 1200ml compound recipe keep-alive liquid; Be by Borneolum Syntheticum 4.8g; SHENGMAI YIN compound recipe 600-750g is prepared from, and wherein, SHENGMAI YIN compound recipe proportioning is 300 parts of Radix Codonopsis, 200 parts of Radix Ophiopogonis, 100 parts of Fructus Schisandrae Chinensis.
Below specify beneficial effect of the present invention through Test Example.
The keep-alive effect experiment of Test Example 1 Aquatic product live keeping agent of the present invention
The live keeping agent for preparing with embodiment 1 carries out the simulate experiment to various aquatic products, for getting rid of other factor affecting, does not use means such as cooling and oxygenation.Set up 1 group of embodiment, blank group, positive controls.When 1 group of embodiment encloses shrimp flower clam and Corbicula fluminea transportation for Misgurni anguillicaudati, astacus, base in every L life water body (25-28 ℃) adding live keeping agent 5ml, put into animal behind the mixing; In every L life water body (25-28 ℃), add live keeping agent 10ml when transporting than large animal, put into animal behind the mixing for builds such as Carassius auratus, Silurus asotus fish, crabs; Matched group adopts conventional water body, does not add live keeping agent, and all the other conditions are with the live keeping agent group.Positive controls is to add MS-2220.02g in every L life water body (25-28 ℃) when enclosing shrimp flower clam and Corbicula fluminea transportation for Misgurni anguillicaudati, astacus, base, puts into animal after the dissolving; In every L life water body (25-28 ℃), add MS-2220.06g when transporting than large animal, put into animal 0.06g behind the mixing, put into animal after the dissolving for builds such as Carassius auratus, Silurus asotus fish, crabs.Live keeping agent is seen table 11-12 to the keep-alive effect of above-mentioned aquatic products.
Table 11 live keeping agent of the present invention is to the keep-alive effect of small aquatic products
Annotate: compare with the blank group
*P＜0.05
*P＜0.01
Table 12 live keeping agent of the present invention is to the keep-alive effect of more large-scale aquatic products
Annotate: compare with the blank group
*P＜0.05
*P＜0.01
The experimental data of simulate shows; Compound recipe live keeping agent group of the present invention and positive MS-222 group compare with blank control group; For being small-sized no matter or the aquatic products of big type of build; Comprise that Fish, shrimps, Eriocheir sinensis class and shellfish all have keep-alive effect clearly, significantly improve the survival rate of the live transport of above-mentioned aquatic products, and live keeping agent of the present invention is more obvious to the more positive MS-222 of keep-alive effect of aquatic products.
The keep-alive effect contrast experiment of Test Example 2 live keeping agents of the present invention and Borneolum Syntheticum, SHENGMAI YIN
Prepare live keeping agent with embodiment 1, and prepare the Borneolum Syntheticum keep-alive liquid and the SHENGMAI YIN keep-alive liquid of single component respectively, aquatic products such as Misgurni anguillicaudati, astacus, Carassius auratus, crab are carried out the simulate experiment,, use means such as cooling and oxygenation for getting rid of other factor affecting.Set up 1 group of embodiment, Borneolum Syntheticum experimental group, SHENGMAI YIN experimental group and blank group.
1 group of embodiment: to toys such as Misgurni anguillicaudati, astacus, in every L life water body (25-28 ℃), add live keeping agent 5ml (embodiment 1 live keeping agent, crude drug concentration is about 0.5g/ml), put into animal behind the mixing; In every L life water body (25-28 ℃), add live keeping agent 10ml when transporting than large animal, put into animal behind the mixing for builds such as Carassius auratus, crabs.
Borneolum Syntheticum experimental group: to toys such as Misgurni anguillicaudati, astacus, in every L life water body (25-28 ℃), add Borneolum Syntheticum 20mg, put into animal after the dissolving; In every L life water body (25-28 ℃), go into Borneolum Syntheticum 40mg when transporting than large animal, put into animal behind the mixing for builds such as Carassius auratus, crabs.
SHENGMAI YIN experimental group: to toys such as Misgurni anguillicaudati, astacus, in every L life water body (25-28 ℃), add SHENGMAI YIN extract 2.5ml (preparation of the same 2.1 SHENGMAI YIN extracting solution of method for making, crude drug concentration are 1g/ml), put into animal behind the mixing; In every L life water body (25-28 ℃), add live keeping agent 5ml when transporting than large animal, put into animal behind the mixing for builds such as Carassius auratus, crabs.
Blank group: in life water body (25-28 ℃), add animal.
The above-mentioned survival of respectively organizing aquatic products is seen table 13.
The research of table 13 live keeping agent compatibility of the present invention effect
Annotate: compare with the blank group
*P＜0.05
*P＜0.01
Above-mentioned experiment can know that keep-alive liquid of the present invention, Borneolum Syntheticum, SHENGMAI YIN group and blank control group relatively all can increase the survival rate of aquatic products, and wherein, live keeping agent group of the present invention is suitable with SHENGMAI YIN group consumption, but effect obviously is superior to single SHENGMAI YIN group of using; Under the suitable situation of Borneolum Syntheticum consumption, the effect of live keeping agent group of the present invention also is superior to single using the Borneolum Syntheticum group, shows behind Borneolum Syntheticum and the SHENGMAI YIN compatibility, can produce synergistic function, with folk prescription mutually specific energy significantly improve the time-to-live and the survival volume of aquatic products.
In sum; Aquatic product keep-alive agent of the present invention is processed by natural Chinese medicines, and institute's medicament has no side effect, and can effectively improve the survival rate of the live transport of various aquatic products; Have advantages such as safe, efficient, for the live transport of aquatic products provides a kind of new selection.
Claims (10)
1. the purposes of the compositions of following weight proportioning in preparation aquatic product keep-alive agent:
Radix Codonopsis 225-375 part, Radix Ophiopogonis 150-250 part, Fructus Schisandrae Chinensis 75-125 part.
2. purposes according to claim 1 is characterized in that: the weight proportion of said compositions is following:
300 parts of Radix Codonopsis, 200 parts of Radix Ophiopogonis, 100 parts of Fructus Schisandrae Chinensis.
3. aquatic product keep-alive agent is characterized in that: it is to be prepared from following materials of weight proportions medicine:
Borneolum Syntheticum 3.6-6 part, Radix Codonopsis 225-375 part, Radix Ophiopogonis 150-250 part, Fructus Schisandrae Chinensis 75-125 part.
4. live keeping agent according to claim 3 is characterized in that: it is to be prepared from following materials of weight proportions medicine:
4.8 parts of Borneolum Syntheticums, Radix Codonopsis 300-375 part, Radix Ophiopogonis 100 1 125 parts of 200-250 part, Fructus Schisandrae Chinensis.
5. according to claim 3 or 4 described live keeping agents; It is characterized in that: it is to be active component by the water of Radix Codonopsis, Radix Ophiopogonis, Fructus Schisandrae Chinensis or extractive with organic solvent; Add the preparation that acceptable auxiliary is prepared from Borneolum Syntheticum and the aquatic product keep-alive agent; Wherein, described adjuvant is emulsifying agent, ethanol, water; Its consumption is following: Borneolum Syntheticum: emulsifying agent is 1: 2-3W/V, Borneolum Syntheticum: ethanol is 1: 10-20W/V, water is an amount of.
6. live keeping agent according to claim 5 is characterized in that: said emulsifying agent is Polysorbate, polyoxyethylene nonylphenol ether, poloxamer, arabic gum, gelatin, phospholipid, tragacanth, Rhizoma Bletillae gel; Preferred Polysorbate.
7. method for preparing any described live keeping agent of claim 3-6, it is characterized in that: it has following operating procedure:
A, by the prescription proportioning get Borneolum Syntheticum, add the emulsifying agent mixing after, add ethanol again, the dissolving, the Borneolum Syntheticum alcoholic solution subsequent use;
B, get Radix Codonopsis, Radix Ophiopogonis, Fructus Schisandrae Chinensis by the prescription proportioning, after the decocte with water, the condensed water decocting liquid, precipitate with ethanol filters; After filtrate recycling ethanol also concentrates, add the Borneolum Syntheticum alcoholic solution of A step preparation again, mixing, thin up; And regulate pH value to 5-7, and filter, get filtrating, promptly get live keeping agent.
8. method according to claim 7 is characterized in that: the concrete grammar of B step is:
Get Radix Codonopsis, Radix Ophiopogonis, Fructus Schisandrae Chinensis, decocte with water 2-3 time, each 1-3 hour, merge decocting liquid, filter; After filtrating concentrates, add ethanol to containing more than the alcohol amount 50%V/V, leave standstill, filter, decompression filtrate recycling ethanol also is concentrated into and contains crude drug 1g/ml; Add the Borneolum Syntheticum alcoholic solution that the A step obtains again, mixing, thin up is regulated pH value to 5-7; Leave standstill, filter, get filtrating, promptly get live keeping agent.
9. the keepalive method of aquatic products, it is any described live keeping agent of claim 3-6 of empoly effective amount, joins in the life water body of aquatic products, mixing gets final product.
10. method according to claim 9 is characterized in that:
Said aquatic products are shrimp, shellfish aquatic products, in life water body, add live keeping agent, to the concentration of Borneolum Syntheticum be 10-20mg/L, preferred 20mg/L; Perhaps
Said aquatic products are fish, Eriocheir sinensis class aquatic products, in life water body, add live keeping agent, to the concentration of Borneolum Syntheticum be 20-40mg/L, preferred 40mg/L.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104920251A (en) * | 2014-12-26 | 2015-09-23 | 浙江省海洋水产研究所 | Paralichthys olivaceus transportation method capable of keeping paralichthys olivaceus alive without water |
| CN105123585A (en) * | 2015-09-11 | 2015-12-09 | 上海婉静纺织科技有限公司 | Aliveness keeping transporting method for crabs |
| CN105166007A (en) * | 2015-09-11 | 2015-12-23 | 上海婉静纺织科技有限公司 | Eriocheir sinensis survival keeping agent |
| CN105941596A (en) * | 2016-05-18 | 2016-09-21 | 青岛博智汇力生物科技有限公司 | Aquatic product temporary rearing and living keeping agent capable of removing heavy metal, use method and application |
| CN107212073A (en) * | 2017-06-15 | 2017-09-29 | 中山火炬职业技术学院 | It is a kind of applied to plant source biological preservation keep-alive liquid of aquatic products and preparation method thereof |
| CN110150212A (en) * | 2018-04-17 | 2019-08-23 | 天津农学院 | It is a kind of reduce Portunus trituberculatus Miers injury of fighting transportation resources and device |
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| CN1279984A (en) * | 2000-06-02 | 2001-01-17 | 贺端澍 | Fish medicine for preventing fish from raising nose above water |
| CN102133332A (en) * | 2011-03-21 | 2011-07-27 | 承德医学院中药研究所 | Traditional Chinese medicine compound preparation for preventing and curing coronary heart disease and angina and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1279984A (en) * | 2000-06-02 | 2001-01-17 | 贺端澍 | Fish medicine for preventing fish from raising nose above water |
| CN102133332A (en) * | 2011-03-21 | 2011-07-27 | 承德医学院中药研究所 | Traditional Chinese medicine compound preparation for preventing and curing coronary heart disease and angina and preparation method thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104920251A (en) * | 2014-12-26 | 2015-09-23 | 浙江省海洋水产研究所 | Paralichthys olivaceus transportation method capable of keeping paralichthys olivaceus alive without water |
| CN105123585A (en) * | 2015-09-11 | 2015-12-09 | 上海婉静纺织科技有限公司 | Aliveness keeping transporting method for crabs |
| CN105166007A (en) * | 2015-09-11 | 2015-12-23 | 上海婉静纺织科技有限公司 | Eriocheir sinensis survival keeping agent |
| CN105941596A (en) * | 2016-05-18 | 2016-09-21 | 青岛博智汇力生物科技有限公司 | Aquatic product temporary rearing and living keeping agent capable of removing heavy metal, use method and application |
| CN107212073A (en) * | 2017-06-15 | 2017-09-29 | 中山火炬职业技术学院 | It is a kind of applied to plant source biological preservation keep-alive liquid of aquatic products and preparation method thereof |
| CN110150212A (en) * | 2018-04-17 | 2019-08-23 | 天津农学院 | It is a kind of reduce Portunus trituberculatus Miers injury of fighting transportation resources and device |
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|---|---|
| CN102430046B (en) | 2013-05-22 |
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