CN102424852B - 实时荧光定量pcr检测方法 - Google Patents
实时荧光定量pcr检测方法 Download PDFInfo
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- CN102424852B CN102424852B CN201110444695.9A CN201110444695A CN102424852B CN 102424852 B CN102424852 B CN 102424852B CN 201110444695 A CN201110444695 A CN 201110444695A CN 102424852 B CN102424852 B CN 102424852B
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Abstract
Description
名称 | 代号 | 序列 | 长度 |
上游引物 | HBV-F1 | 5’-GTG TCT GCG GCG TTT TAT CAT-3’ | 21bp |
下游引物 | HBV-R1 | 5’-ACA AAC GGG CAA CAT ACC TTG-3’ | 21bp |
第一探针 | HBV-P1 | 5’FAM-CAT CCT GCT GCT ATG CCT CAT CTT CTT-Dabcyl 3’ | 27bp |
增补探针 | ICP1 | 5’HEX-CCTCATCTTCTTGTTGGTTCTTCTGGACT-Dabcyl 3’ | 29bp |
内标探针 | ICP2 | 5’HEX-TCATCCAGTGCAAGTCTTGATCCTGTC-Dabcyl 3’ | 27bp |
名称 | 试剂厂家 | 浓度 |
10×PCRbuffer | 上海Promega公司 | |
dNTPs | 上海Promega公司 | A、T、C、G各8nmol |
HBV-F1 | Invetrogen公司合成 | 10pmol |
HBV-R1 | Invetrogen公司合成 | 10pmol |
HBV-P1 | Invetrogen公司合成 | 15pmol |
ICP1 | Invetrogen公司合成 | 5pmol |
ICP2 | Invetrogen公司合成 | 10pmol |
H-taq | 上海Promega公司 | 5U |
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CN201110444695.9A CN102424852B (zh) | 2011-12-27 | 2011-12-27 | 实时荧光定量pcr检测方法 |
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CN102424852A CN102424852A (zh) | 2012-04-25 |
CN102424852B true CN102424852B (zh) | 2014-04-02 |
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Publication number | Priority date | Publication date | Assignee | Title |
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JP5944999B2 (ja) * | 2012-07-03 | 2016-07-05 | マッカイ メモリアル ホスピタルMackay Memorial Hospital | 自己競合型プライマーおよびこの自己競合型プライマーの使用方法 |
CN103468829B (zh) * | 2013-09-25 | 2016-05-25 | 北京普利耐特生物科技有限公司 | 一种检测乙肝病毒核酸试剂盒 |
WO2016013115A1 (ja) * | 2014-07-25 | 2016-01-28 | 株式会社 リージャー | 希釈生体試料成分の分析方法 |
CN105296666A (zh) * | 2015-12-07 | 2016-02-03 | 湖南圣维基因科技有限公司 | 一种braf基因v600e突变荧光pcr检测试剂盒 |
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CN101475988A (zh) * | 2009-02-03 | 2009-07-08 | 戴立忠 | 一种实时荧光定量pcr实验内标的设计方法 |
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