CN102422773A - Separating, domesticating and culturing method for wild white glossy ganoderma - Google Patents
Separating, domesticating and culturing method for wild white glossy ganoderma Download PDFInfo
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- CN102422773A CN102422773A CN2011102521480A CN201110252148A CN102422773A CN 102422773 A CN102422773 A CN 102422773A CN 2011102521480 A CN2011102521480 A CN 2011102521480A CN 201110252148 A CN201110252148 A CN 201110252148A CN 102422773 A CN102422773 A CN 102422773A
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Abstract
The invention discloses a separating, domesticating and culturing method for wild white glossy ganoderma. The method is characterized by comprising the following steps of collecting glossy ganoderma; separating the collected glossy ganoderma; inoculating the isolate on a separating culture medium; placing the separating culture medium and the isolate in a tube; culturing the separating culture medium and the isolate till growing white fluffy hypha; inoculating the hypha on bag-shaped sterilized foster body culture medium; maintaining the temperature of 20-30 DEG C and relative humidity of 60-90%; ventilating to culture fruit body hypha; performing fruit body culturing for the fruit body hypha in a greenhouse with the temperature of 15-25 DEG C and relative humidity of 75-95%. Compared with the prior art, the method provided by the invention has the advantages of high survival rate and large fruit body.
Description
Technical field
The present invention relates to a kind of separation standardization and cultural method of mushroom, separation standardization of especially wild white glossy ganoderma and cultural method.
Background technology
The characteristics of white Ganoderma lucidum mycelium, white Ganoderma lucidum mycelium shows as white mycelium in parent, sturdy, be fine hair shape, calm growth, climb the wall phenomenon after covering with medium slant.At 23-25 ℃, cultivated 8-10 days, cover with the inclined-plane of medium.
Existing wild white glossy ganoderma artificial culture survival rate is lower, generally can reach 60-70%.
Summary of the invention
The object of the present invention is to provide separation standardization and the cultural method of a kind of wild white glossy ganoderma, it has the survival rate height, many advantages of deriving.
For achieving the above object, the technical scheme that the present invention adopted is following:
1, separation standardization of a kind of wild white glossy ganoderma and cultural method is characterized in that may further comprise the steps:
A, choose that the sesame body is plump, cap is big and the Wild ganoderma of circle is the seed collecting target;
B, the glossy ganoderma of gathering is separated immediately;
C, above-mentioned separator is seeded on the isolation medium, isolation medium and separator are inserted in the pipe, be cultured to and grow white fine hair shape mycelia;
D, above-mentioned original seed mycelia is connected to educating on the body medium after the sterilization of bag shape, keeps 20-30 ℃ of temperature, relative moisture 60-90% ventilates and cultivates the fruit body mycelia;
E, word entity mycelia is carried out entity cultivate in booth, temperature 15-25 degree centigrade, relative moisture 75-95%.
Above-mentioned method, among the said step D, temperature is 24-28 ℃, relative moisture 75-80%; In the said step e, temperature is 16-23 ℃, relative moisture 85-95%.
Above-mentioned method describedly is separated into that separate tissue, matrix are separated, at least a in the spore separation; Isolation medium among the step C is a potato dextrose agar.
Above-mentioned method, the said body culture medium prescription of educating is following: cotton seed hulls 100 mass parts, wood chip 35 mass parts, white sugar 1 mass parts, wheat bran 6 quality, land plaster 1 mass parts.
Above-mentioned method, the said body moisture content in medium 60-70% that educates, pH value is 5-6.
Above-mentioned method, said booth is designed to: back wall is high 3 meters, front wall is high 1.5 meters, grow 15 meters, and wide 9 meters, back wall is provided with 7 ventilating openings, and each is of a size of 50CM*50CM; The row's of being provided with bag frame in the said booth, ground level is 50 centimetres in row's bag frame, and 0.4-0.5 centimetre space is arranged between the bacterium bag.
Beneficial effect of the present invention shows:
White Ganoderma lucidum mycelium grows to need to cultivate and keeps certain pH value, and this is a necessary condition of keeping protoplasm life, and PH is too high or cross the low growth that all influences mycelia.
Temperature should not be too high, and too high button flavescence begins to stop growing, and temperature is too low, and fruit body should not be launched.
Ventilation, improper ventilation, fruit body should not be launched, and the bad temp. and humidity in addition of ventilating changes, and fruit body very easily forms knob.
Adopt separating method immediately, can keep the activity of white glossy ganoderma, improve the success rate of cultivating.
Size is set booth and relevant setting of arranging the bag frame can be with white ganoderma lucidum fruitbody according to arranging as in the booth, and fruit body can not increase the cultivation amount of fruit body too much perhaps very little to greatest extent in the situation of the survival rate that guarantees fruit body.
Embodiment
Come the invention is described in detail with embodiment below:
Separation standardization of a kind of wild white glossy ganoderma and cultural method may further comprise the steps:
A, choose that the sesame body is plump, cap is big and the Wild ganoderma of circle is the seed collecting target;
B, the glossy ganoderma of gathering is carried out breeding immediately separate, this breeding separates can adopt that separate tissue, matrix are separated, the form of one or more combinations of spore separation;
C, above-mentioned separator is seeded on the potato dextrose agar, isolation medium and separator are inserted in the pipe, one of a pipe is cultivated a Zhou Houzhi and is grown white fine hair shape mycelia;
D, making medium: the material of cotton seed hulls 100 mass parts, wood chip 35 mass parts, white sugar 1 mass parts, wheat bran 6 quality, land plaster 1 mass parts is added water mixing stirring; Make that mixing the back water content is about 65%; And the pH value of material is adjusted to 5-6, pack, the sterilization 8 hours down of normal pressure kitchen range;
Above-mentioned original seed mycelia is connected to educating on the body medium after the sterilization of bag shape, keeps 24-28 ℃ of temperature, relative moisture 75-80% ventilates and cultivates the fruit body mycelia;
The fruit body mycelia is carried out entity cultivate in booth, temperature 16-23 degree centigrade, relative moisture 85-95%.
Wherein the booth size is following: back wall is high 3 meters, front wall is high 1.5 meters, grow 15 meters, and wide 9 meters, back wall is provided with 7 ventilating openings, and each is of a size of 50CM*50CM; The row's of being provided with bag frame in the booth, ground level is 50 centimetres in row's bag frame, and 0.4-0.5 centimetre space is arranged between the bacterium bag.
After adopting this method, yield rate reaches 93%, and other indexs will obviously be superior to wild size such as the size of cap, can reach wild 1.5 times, and taste, nutrient component are compared wild indifference.
Claims (9)
1. separation standardization of a wild white glossy ganoderma and cultural method is characterized in that may further comprise the steps:
Choosing the Wild ganoderma that the sesame body is plump, cap is big and justify is the seed collecting target;
The glossy ganoderma of gathering is separated immediately;
Above-mentioned separator is seeded on the isolation medium, isolation medium and separator are inserted in the pipe, be cultured to and grow white fine hair shape mycelia;
Above-mentioned original seed mycelia is connected to educating on the body medium after the sterilization of bag shape, keeps 20-30 ℃ of temperature, relative moisture 60-90% ventilates and cultivates the fruit body mycelia;
Word entity mycelia is carried out entity cultivate in booth, temperature 15-25 degree centigrade, relative moisture 75-95%.
2. according to right 1 described method, it is characterized in that: among the said step D, temperature is 24-28 ℃, relative moisture 75-80%.
3. according to right 1 described method, it is characterized in that: in the said step e, temperature is 16-23 ℃, relative moisture 85-95%.
4. according to right 1 described method, it is characterized in that: describedly be separated into that separate tissue, matrix are separated, at least a in the spore separation.
5. according to right 1 described method, it is characterized in that: the isolation medium among the step C is a potato dextrose agar.
6. method according to claim 1 is characterized in that: the said body culture medium prescription of educating is following: cotton seed hulls 100 mass parts, wood chip 35 mass parts, white sugar 1 mass parts, wheat bran 6 quality, land plaster 1 mass parts.
7. according to claim 1 or 6 described methods, it is characterized in that: the said body moisture content in medium 60-70% that educates, pH value is 5-6.
8. method according to claim 1 is characterized in that: said booth is designed to: back wall is high 3 meters, front wall is high 1.5 meters, grow 15 meters, and wide 9 meters, back wall is provided with 7 ventilating openings, and each is of a size of 50CM*50CM.
9. according to claim 1 or 8 described methods, it is characterized in that: the row's of being provided with bag frame in the said booth, ground level is 50 centimetres in row's bag frame, and 0.4-0.5 centimetre space is arranged between the bacterium bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102521480A CN102422773A (en) | 2011-08-30 | 2011-08-30 | Separating, domesticating and culturing method for wild white glossy ganoderma |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102521480A CN102422773A (en) | 2011-08-30 | 2011-08-30 | Separating, domesticating and culturing method for wild white glossy ganoderma |
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| CN102422773A true CN102422773A (en) | 2012-04-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2011102521480A Pending CN102422773A (en) | 2011-08-30 | 2011-08-30 | Separating, domesticating and culturing method for wild white glossy ganoderma |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103355101A (en) * | 2013-07-27 | 2013-10-23 | 何寒 | Lucid ganoderma stock culture and preparation method thereof |
| CN103739349A (en) * | 2013-12-09 | 2014-04-23 | 广西柳城县成霖农业科技有限公司 | Fungus bags used for bag cultivation of lucid ganoderma |
| CN104956914A (en) * | 2015-06-18 | 2015-10-07 | 唐伟 | Breeding method for natural ganoderma lucidum |
-
2011
- 2011-08-30 CN CN2011102521480A patent/CN102422773A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103355101A (en) * | 2013-07-27 | 2013-10-23 | 何寒 | Lucid ganoderma stock culture and preparation method thereof |
| CN103739349A (en) * | 2013-12-09 | 2014-04-23 | 广西柳城县成霖农业科技有限公司 | Fungus bags used for bag cultivation of lucid ganoderma |
| CN104956914A (en) * | 2015-06-18 | 2015-10-07 | 唐伟 | Breeding method for natural ganoderma lucidum |
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Application publication date: 20120425 |