CN102406668A - Preparation method of hypocrella bambusae extract - Google Patents
Preparation method of hypocrella bambusae extract Download PDFInfo
- Publication number
- CN102406668A CN102406668A CN2011103944872A CN201110394487A CN102406668A CN 102406668 A CN102406668 A CN 102406668A CN 2011103944872 A CN2011103944872 A CN 2011103944872A CN 201110394487 A CN201110394487 A CN 201110394487A CN 102406668 A CN102406668 A CN 102406668A
- Authority
- CN
- China
- Prior art keywords
- hypocrella bambusae
- sace
- bet
- hypocrella
- bambusae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a preparation method of hypocrella bambusae extract. The preparation method comprises the following steps in sequence: (1) material preparation: handpicking hypocrella bambusae; (2) smashing: smashing hypocrella bambusae into coarse granules or coarse powder; (3) cold soaking extraction: putting hypocrella bambusae smashed into coarse granules or coarse powder into a multifunctional extraction pot, adding 90% or above ethanol, the weight of which is 4-8 times that of hypocrella bambusae, and carrying out cold soaking for 12-24 hours twice to three times; and (4) condensation:mixing extracting solution in a vacuum tank to recover ethanol until the specific weight of condensed solution is 1.2-1.6, thus the hypocrella bambusae extract is obtained. According to the invention, during extraction, the condensation speed is high, the water content of the extract is relatively low, the property and appearance of the extract are better, the yield is relatively high, the safety is improved and energy is relatively saved.
Description
Technical field
The present invention relates to a kind of Hypocrella bambusae (Bet Br). Sace extract making method.
Background technology
Hypocrella bambusae (Bet Br). Sace (Hypocrella bambusae (B.et.Br) Sacc) belongs to Ascomycetes, Hypocreales, Hypocreaceae; It is a kind of natural bacterium that colonizes in Folium Bambusae stalk portion; Originate in areas such as Yunnan Lijing; Its steep in wine orally taken for curing stomachache and arthritis commonly used among the people has exsiccant Hypocrella bambusae (Bet Br). Sace to sell on the medical material market.Be to improve the use drug effect of Hypocrella bambusae (Bet Br). Sace, the method that prior art adopts alcohol heat reflux to extract is prepared into Hypocrella bambusae (Bet Br). Sace extractum with Hypocrella bambusae (Bet Br). Sace, as the Chinese patent bamboo parasitic fungus extract and its production and application (publication number: CN1284494A; Open day February 21 calendar year 2001), disclose a kind of method for preparing of bamboo parasitic fungus extract, this method is a raw material with the Hypocrella bambusae (Bet Br). Sace coarse powder; Drop in the multi-function extractor; The alcohol heat reflux of adding 75～85%, forced circulation is extracted twice; Merging extracted twice liquid reclaims ethanol to concentrated solution proportion in vacuum tank be 1.1～1.4; The acetone of 4～6 times of weight of adding stirring and refluxing while hot left standstill and is cooled to room temperature after 8～15 minute, filtered and reclaimed acetone and promptly get bamboo parasitic fungus extract.This method is owing to adopt the alcohol heat reflux method, and operation is trouble, also is unfavorable for energy-conservationly, and this method adopts virose acetone to make extractant, so production security reduces.
In addition, the red bamboo fungus stroma diameter that on market, can buy at present is 3～12mm, is significantly less than 7～20 mm; Mostly Stroma inside is baby pink, reaches peony and only accounts for 10～20%, and raw materials quality obviously descends; Content reduces, and adopts prior art alcohol heat reflux method for distilling, and its extraction ratio is low.
Summary of the invention:
To the above-mentioned deficiency of prior art, the object of the present invention is to provide that a kind of technology is simple, recovery rate is high, and safety improves, more energy-conservation Hypocrella bambusae (Bet Br). Sace extract making method.
The objective of the invention is to realize through following technical scheme: 1, a kind of Hypocrella bambusae (Bet Br). Sace extract making method is characterized in that being made up of the step of following order:
(1) gets the raw materials ready: selected Hypocrella bambusae (Bet Br). Sace;
(2) pulverize: Hypocrella bambusae (Bet Br). Sace is ground into coarse granule or coarse powder;
(3) merceration extracts: the Hypocrella bambusae (Bet Br). Sace that will be ground into coarse granule or coarse powder drops in the multi-function extractor, and the 90% above ethanol that adds 4～6 times of amount weight is distinguished merceration 2～3 times, each 12～24 hours;
(4) concentrate: merge extractive liquid, reclaims ethanol to concentrated solution proportion in vacuum tank be 1.2～1.6, promptly makes Hypocrella bambusae (Bet Br). Sace extractum.
The described selected red bamboo fungus stroma diameter of said step (1) is 10 ~ 12mm, and the inner peony of Stroma accounts for 15% ~ 18%.
The invention has the beneficial effects as follows: because the method that the present invention adopts 90% above ethanol merceration to extract, processing step has only pulverizing and merceration to extract for two steps, reduces than prior art step; Concentrate fast during extraction; The extractum water content is lower, and character and outward appearance are better, and recovery rate is higher; Since do not use alcohol heating reflux method of the prior art, therefore simple to operate, more energy-conservation; Owing to do not select for use virose organic solvent such as acetone as extractant, method for distilling safety therefore of the present invention improves.
The specific embodiment
Below in conjunction with embodiment the present invention is further described, but be not limited to embodiment.
Embodiment 1
With commercially available Hypocrella bambusae (Bet Br). Sace is raw material, and the red bamboo fungus stroma diameter is 10mm, and the inner peony of Stroma accounts for 15%, and it is ground into coarse granule; Taking by weighing Hypocrella bambusae (Bet Br). Sace coarse granule 250kg drops in the multi-function extractor; Add 90% ethanol merceration twice respectively, Hypocrella bambusae (Bet Br). Sace and alcoholic acid weight ratio are 1:11, measure 24 hours for 5 times for the first time; Measured 24 hours for 6 times for the second time; Merging extracted twice liquid reclaims ethanol to concentrated solution proportion in vacuum concentration pot be 1.2 (recording under 80 ℃), promptly gets Hypocrella bambusae (Bet Br). Sace extractum, and content of hypocrellin A and ultraviolet thereof and visible region scan feature are seen table 1 in the Hypocrella bambusae (Bet Br). Sace extractum.
Embodiment 2
With commercially available Hypocrella bambusae (Bet Br). Sace is raw material, and the red bamboo fungus stroma diameter is 12mm, and the inner peony of Stroma accounts for 18%, and it is ground into coarse powder; Taking by weighing Hypocrella bambusae (Bet Br). Sace coarse powder 250kg drops in the multi-function extractor; Add 95% ethanol difference merceration 3 times, Hypocrella bambusae (Bet Br). Sace and alcoholic acid weight ratio are 1:12, measure 12 hours for 4 times for the first time; Measured 12 hours for 4 times for the second time; 4 times of amounts are 12 hours for the third time, and merging 3 extracting solution, in vacuum concentration pot, to reclaim ethanol to concentrated solution proportion be 1.6 (80 ℃ record), promptly gets Hypocrella bambusae (Bet Br). Sace extractum.Content of hypocrellin A and ultraviolet thereof and visible region scan feature are seen table 1 in the Hypocrella bambusae (Bet Br). Sace extractum.
The prior art convention
With commercially available Hypocrella bambusae (Bet Br). Sace is raw material, and the red bamboo fungus stroma diameter is 10mm, and the inner peony of Stroma accounts for 15%, and it is ground into coarse powder; Take by weighing Hypocrella bambusae (Bet Br). Sace coarse powder 250kg and drop in the multi-function extractor, add 80% alcohol heat reflux, Hypocrella bambusae (Bet Br). Sace and alcoholic acid weight ratio are 1:12; Measured 2 hours for 6 times for the first time, measured 2 hours for 6 times for the second time, the merging extracted twice is filtrated and in vacuum concentration pot, reclaimed ethanol to concentrated solution proportion is 1.1; The acetone that adds 5 times of amounts is stirring and refluxing after 15 minutes while hot; Leave standstill and be cooled to room temperature, filter, get Hypocrella bambusae (Bet Br). Sace extractum behind the recovery acetone.Content of hypocrellin A and ultraviolet thereof and visible region scan feature are seen table 1 in the Hypocrella bambusae (Bet Br). Sace extractum.
Content of hypocrellin A and ultraviolet thereof and visible region scan feature in the table 1 Hypocrella bambusae (Bet Br). Sace extractum
Embodiment 1 and 2 and the scan feature of prior art convention be in 200-700nm scanning, observe absorbing state at ultraviolet and visible region.Experiment is to record through TU-1800PC (ultraviolet-uisible spectrophotometer), and wavelength width is 1nm, and the sampling interval is 0.20 nm.Can find out that from table 1 maximum absorption wavelength of embodiment 1 and embodiment 2 is 464 nm, 465 nm match with standard, and content of hypocrellin A reaches 4.4～4.9%; The prior art convention adopts the alcohol heat reflux method for distilling of prior art, and its maximum absorption wavelength is 461nm, with standard value minor shifts is arranged, and content of hypocrellin A has only 1.7%.
Claims (2)
1. a Hypocrella bambusae (Bet Br). Sace extract making method is characterized in that, is made up of the step of following order:
(1) gets the raw materials ready: selected Hypocrella bambusae (Bet Br). Sace;
(2) pulverize: Hypocrella bambusae (Bet Br). Sace is ground into coarse granule or coarse powder;
(3) merceration extracts: the Hypocrella bambusae (Bet Br). Sace that will be ground into coarse granule or coarse powder drops in the multi-function extractor, adds 4～6 times of amounts, 90% above ethanol difference merceration 2～3 times, each 12～24 hours;
(4) concentrate: merge extractive liquid, reclaims ethanol to concentrated solution proportion in vacuum tank be 1.2～1.6, promptly makes Hypocrella bambusae (Bet Br). Sace extractum.
2. Hypocrella bambusae (Bet Br). Sace extract making method according to claim 1 is characterized in that, the described selected red bamboo fungus stroma diameter of said step (1) is 10 ~ 12mm, and the inner peony of Stroma accounts for 15% ~ 18%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110394487 CN102406668B (en) | 2011-12-02 | 2011-12-02 | Preparation method of hypocrella bambusae extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110394487 CN102406668B (en) | 2011-12-02 | 2011-12-02 | Preparation method of hypocrella bambusae extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102406668A true CN102406668A (en) | 2012-04-11 |
| CN102406668B CN102406668B (en) | 2013-01-30 |
Family
ID=45909206
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110394487 Active CN102406668B (en) | 2011-12-02 | 2011-12-02 | Preparation method of hypocrella bambusae extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102406668B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103006716A (en) * | 2012-12-28 | 2013-04-03 | 昆明振华制药厂有限公司 | Hypocrellin external preparation for treating leucoderma |
| CN113149819A (en) * | 2021-05-18 | 2021-07-23 | 山东国力生物科技有限公司 | Method for extracting hypocrellin from solid-state fermentation material of tabasheer fungus |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1284494A (en) * | 2000-08-22 | 2001-02-21 | 云南医药工业股份有限公司 | Bamboo parasitic fungus extract and its preparation and application |
-
2011
- 2011-12-02 CN CN 201110394487 patent/CN102406668B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1284494A (en) * | 2000-08-22 | 2001-02-21 | 云南医药工业股份有限公司 | Bamboo parasitic fungus extract and its preparation and application |
Non-Patent Citations (2)
| Title |
|---|
| 《云南中医学院学报》 20100630 郑立雄等 "云南民族药物竹红菌的化学成分研究" 25-29 1，2 第33卷, 第3期 * |
| 郑立雄等: ""云南民族药物竹红菌的化学成分研究"", 《云南中医学院学报》, vol. 33, no. 3, 30 June 2010 (2010-06-30), pages 25 - 29 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103006716A (en) * | 2012-12-28 | 2013-04-03 | 昆明振华制药厂有限公司 | Hypocrellin external preparation for treating leucoderma |
| CN113149819A (en) * | 2021-05-18 | 2021-07-23 | 山东国力生物科技有限公司 | Method for extracting hypocrellin from solid-state fermentation material of tabasheer fungus |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102406668B (en) | 2013-01-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101928639B (en) | Natural tea essence prepared by using tea and preparation method thereof | |
| CN101986855A (en) | Method for preparing low-caffeine high-tea polyphenol tea powder | |
| CN101999493B (en) | Preparation method of plant health-care cool tea | |
| CN103564595A (en) | Instant solid lotus leaf beverage as well as processing method thereof | |
| CN103039653A (en) | Production method of instant honeysuckle tea | |
| CN103859098A (en) | Preparation method for green tea type extract | |
| CN102919422B (en) | Process for extracting herbal tea concentrate | |
| CN103704420A (en) | Processing method of instant tea powder | |
| CN102040579A (en) | Method for extracting luteolin from peanut roots, stems, leaves and shells | |
| CN103976453A (en) | Chuzhou chrysanthemum extract, solid beverage, lozenges and food additive as well as preparation method of Chuzhou chrysanthemum extract | |
| CN101391951A (en) | Production preparation process of high-purity shikimic acid | |
| CN101570581A (en) | Method for comprehensively extracting pueraria polysaccharide and flavone | |
| CN102772521B (en) | Method for extracting coffee polyphenols from coffee bean | |
| CN102406668B (en) | Preparation method of hypocrella bambusae extract | |
| CN103193832A (en) | Method for extracting and separating high-purity tea polyphenol from tea leaves | |
| CN103356740B (en) | Preparation method of baicalein and scutellaria baicalensis flavone total-aglycone extractives | |
| CN101492350B (en) | Method for producing D-pinit from plant locust | |
| CN102002083A (en) | Method for extracting high-purity rutin with flash-extraction technology | |
| CN104606288A (en) | Novel method for preparing total flavonoid aglycone extract of scutellaria baicalensis georgi | |
| CN102061219A (en) | Method for extracting tea seed oil | |
| CN102047997A (en) | Wuyi Mountain rock tea extract, and preparation method and application thereof | |
| CN103059158A (en) | Method for inhibiting foam generation in tea flower polysaccharide extraction | |
| CN102552363A (en) | Preparation method for mother chrysanthemum extract | |
| CN104045523A (en) | Method for extracting and separating resveratrol from polygonum cuspidatum root | |
| CN102731309B (en) | A kind of method extracting separating chlorogenic acid from Herba Dendranthematis indici |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |