CN102406668B - Preparation method of hypocrella bambusae extract - Google Patents
Preparation method of hypocrella bambusae extract Download PDFInfo
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- CN102406668B CN102406668B CN 201110394487 CN201110394487A CN102406668B CN 102406668 B CN102406668 B CN 102406668B CN 201110394487 CN201110394487 CN 201110394487 CN 201110394487 A CN201110394487 A CN 201110394487A CN 102406668 B CN102406668 B CN 102406668B
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- bambusae
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Abstract
The invention relates to a preparation method of hypocrella bambusae extract. The preparation method comprises the following steps in sequence: (1) material preparation: handpicking hypocrella bambusae; (2) smashing: smashing hypocrella bambusae into coarse granules or coarse powder; (3) cold soaking extraction: putting hypocrella bambusae smashed into coarse granules or coarse powder into a multifunctional extraction pot, adding 90% or above ethanol, the weight of which is 4-8 times that of hypocrella bambusae, and carrying out cold soaking for 12-24 hours twice to three times; and (4) condensation:mixing extracting solution in a vacuum tank to recover ethanol until the specific weight of condensed solution is 1.2-1.6, thus the hypocrella bambusae extract is obtained. According to the invention, during extraction, the condensation speed is high, the water content of the extract is relatively low, the property and appearance of the extract are better, the yield is relatively high, the safety is improved and energy is relatively saved.
Description
Technical field
The present invention relates to a kind of preparation method of Hypocrella bambusae (Bet Br). Sace extractum.
Background technology
Hypocrella bambusae (Bet Br). Sace (Hypocrella bambusae (B.et.Br) Sacc) belongs to Ascomycetes, Hypocreales, Hypocreaceae, it is a kind of natural bacterium that colonizes in Folium Bambusae stalk section, originate in the areas such as Lijiang, yunnan, its steep in wine orally taken for curing stomachache and arthritis commonly used among the people has dry Hypocrella bambusae (Bet Br). Sace to sell on the medical material market.For improving the use drug effect of Hypocrella bambusae (Bet Br). Sace, the method that prior art adopts alcohol heat reflux to extract is prepared into Hypocrella bambusae (Bet Br). Sace extractum with Hypocrella bambusae (Bet Br). Sace, such as Chinese patent bamboo parasitic fungus extract and its preparation method and application (publication number: CN1284494A, open day February 21 calendar year 2001), a kind of preparation method of bamboo parasitic fungus extract is disclosed, this method is take the Hypocrella bambusae (Bet Br). Sace coarse powder as raw material, drop in the multi-function extractor, the alcohol heat reflux of adding 75～85%, forced circulation, extract twice, merging extracted twice liquid Recycled ethanol to concentrated solution proportion in vacuum tank is 1.1～1.4, the acetone of 4～6 times of weight of adding while hot stirring and refluxing left standstill and is cooled to room temperature after 8～15 minute, filtered and reclaimed acetone and namely get bamboo parasitic fungus extract.The method is owing to adopt the alcohol heat reflux method, and operation is trouble, also is unfavorable for energy-conservationly, and the method adopts virose acetone to make extractant, so production security reduces.
In addition, the red bamboo fungus stroma diameter that can buy on market at present is 3～12mm, is significantly less than 7～20 mm, Stroma inside mostly is baby pink, reaches peony and only accounts for 10～20%, and raw materials quality obviously descends, content adopts prior art alcohol heat reflux extracting method, and its extraction ratio is low.
Summary of the invention:
For the above-mentioned deficiency of prior art, the object of the present invention is to provide that a kind of technique is simple, recovery rate is high, and the preparation method of the Hypocrella bambusae (Bet Br). Sace extractum of safety raising, relative energy-saving.
The objective of the invention is to be achieved through the following technical solutions: 1, a kind of preparation method of Hypocrella bambusae (Bet Br). Sace extractum is characterized in that being comprised of the step of following order:
(1) gets the raw materials ready: selected Hypocrella bambusae (Bet Br). Sace;
(2) pulverize: Hypocrella bambusae (Bet Br). Sace is ground into coarse granule or coarse powder;
(3) merceration extracts: the Hypocrella bambusae (Bet Br). Sace that will be ground into coarse granule or coarse powder drops in the multi-function extractor, and the 90% above ethanol that adds 4～6 times of amount weight is distinguished merceration 2～3 times, each 12～24 hours;
(4) concentrated: merge extractive liquid, Recycled ethanol to concentrated solution proportion in vacuum tank is 1.2～1.6, namely makes Hypocrella bambusae (Bet Br). Sace extractum.
The described selected red bamboo fungus stroma diameter of described step (1) is 10 ~ 12mm, and the inner peony of Stroma accounts for 15% ~ 18%.
The invention has the beneficial effects as follows: because the method that the present invention adopts 90% above ethanol merceration to extract, processing step only has pulverizing and merceration to extract for two steps, reduces than prior art step, concentrated fast during extraction, the extractum water content is lower, and character and outward appearance are better, and recovery rate is higher; Since do not use alcohol heating reflux method of the prior art, therefore simple to operate, relative energy-saving; Owing to do not select the virose organic solvent such as acetone as extractant, extracting method safety therefore of the present invention improves.
The specific embodiment
The invention will be further described below in conjunction with embodiment, but be not limited to embodiment.
Embodiment 1
Take commercially available Hypocrella bambusae (Bet Br). Sace as raw material, the red bamboo fungus stroma diameter is 10mm, and the inner peony of Stroma accounts for 15%, and it is ground into coarse granule; Taking by weighing Hypocrella bambusae (Bet Br). Sace coarse granule 250kg drops in the multi-function extractor, add twice of 90% ethanol difference merceration, the weight ratio of Hypocrella bambusae (Bet Br). Sace and ethanol is 1:11, measured 24 hours for 5 times for the first time, measured 24 hours for 6 times for the second time, merging extracted twice liquid Recycled ethanol to concentrated solution proportion in vacuum concentration pot is 1.2 (recording under 80 ℃), namely gets Hypocrella bambusae (Bet Br). Sace extractum, and content of hypocrellin A and Uv and visible light domain scanning feature thereof see Table 1 in the Hypocrella bambusae (Bet Br). Sace extractum.
Embodiment 2
Take commercially available Hypocrella bambusae (Bet Br). Sace as raw material, the red bamboo fungus stroma diameter is 12mm, and the inner peony of Stroma accounts for 18%, and it is ground into coarse powder; Taking by weighing Hypocrella bambusae (Bet Br). Sace coarse powder 250kg drops in the multi-function extractor, add 95% ethanol difference merceration 3 times, the weight ratio of Hypocrella bambusae (Bet Br). Sace and ethanol is 1:12, measured 12 hours for 4 times for the first time, measured 12 hours for 4 times for the second time, measured 12 hours for 4 times for the third time, merging 3 extracting solution Recycled ethanol to concentrated solution proportion in vacuum concentration pot is 1.6 (80 ℃ record), namely gets Hypocrella bambusae (Bet Br). Sace extractum.Content of hypocrellin A and Uv and visible light domain scanning feature thereof see Table 1 in the Hypocrella bambusae (Bet Br). Sace extractum.
The prior art convention
Take commercially available Hypocrella bambusae (Bet Br). Sace as raw material, the red bamboo fungus stroma diameter is 10mm, and the inner peony of Stroma accounts for 15%, and it is ground into coarse powder; Taking by weighing Hypocrella bambusae (Bet Br). Sace coarse powder 250kg drops in the multi-function extractor, add 80% alcohol heat reflux, the weight ratio of Hypocrella bambusae (Bet Br). Sace and ethanol is 1:12, measured 2 hours for 6 times for the first time, measured 2 hours for 6 times for the second time, merging extracted twice filtrate Recycled ethanol to concentrated solution proportion in vacuum concentration pot is 1.1, the acetone that adds 5 times of amounts is stirring and refluxing after 15 minutes while hot, leave standstill and be cooled to room temperature, filter, get Hypocrella bambusae (Bet Br). Sace extractum behind the recovery acetone.Content of hypocrellin A and Uv and visible light domain scanning feature thereof see Table 1 in the Hypocrella bambusae (Bet Br). Sace extractum.
Content of hypocrellin A and Uv and visible light domain scanning feature thereof in the table 1 Hypocrella bambusae (Bet Br). Sace extractum
Embodiment 1 and 2 and the scan feature of prior art convention be in 200-700nm scanning, observe the absorbing state in the Uv and visible light district.Experiment is by the TU-1800PC(ultraviolet-uisible spectrophotometer) record, wavelength width is 1nm, the sampling interval is 0.20 nm.As can be seen from Table 1, the maximum absorption wavelength of embodiment 1 and embodiment 2 is 464 nm, and 465 nm match with standard, and content of hypocrellin A reaches 4.4～4.9%; The prior art convention adopts the alcohol heat reflux extracting method of prior art, and its maximum absorption wavelength is 461nm, with standard value minor shifts is arranged, and content of hypocrellin A only has 1.7%.
Claims (2)
1. the preparation method of a Hypocrella bambusae (Bet Br). Sace extractum is characterized in that, is comprised of the step of following order:
(1) gets the raw materials ready: selected Hypocrella bambusae (Bet Br). Sace;
(2) pulverize: Hypocrella bambusae (Bet Br). Sace is ground into coarse granule or coarse powder;
(3) merceration extracts: the Hypocrella bambusae (Bet Br). Sace that will be ground into coarse granule or coarse powder drops in the multi-function extractor, adds 4～6 times of amounts, 90% above ethanol difference merceration 2～3 times, each 12～24 hours;
(4) concentrated: merge extractive liquid, Recycled ethanol to concentrated solution proportion in vacuum tank is 1.2～1.6, namely makes Hypocrella bambusae (Bet Br). Sace extractum.
2. the preparation method of Hypocrella bambusae (Bet Br). Sace extractum according to claim 1 is characterized in that, the described selected red bamboo fungus stroma diameter of described step (1) is 10 ~ 12mm, and the inner peony of Stroma accounts for 15% ~ 18%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110394487 CN102406668B (en) | 2011-12-02 | 2011-12-02 | Preparation method of hypocrella bambusae extract |
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| CN 201110394487 CN102406668B (en) | 2011-12-02 | 2011-12-02 | Preparation method of hypocrella bambusae extract |
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| CN102406668A CN102406668A (en) | 2012-04-11 |
| CN102406668B true CN102406668B (en) | 2013-01-30 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103006716A (en) * | 2012-12-28 | 2013-04-03 | 昆明振华制药厂有限公司 | Hypocrellin external preparation for treating leucoderma |
| CN113149819B (en) * | 2021-05-18 | 2022-04-15 | 山东国力生物科技有限公司 | Method for extracting hypocrellin from solid-state fermentation material of tabasheer fungus |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1284494A (en) * | 2000-08-22 | 2001-02-21 | 云南医药工业股份有限公司 | Bamboo parasitic fungus extract and its preparation and application |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1284494A (en) * | 2000-08-22 | 2001-02-21 | 云南医药工业股份有限公司 | Bamboo parasitic fungus extract and its preparation and application |
Non-Patent Citations (2)
| Title |
|---|
| "云南民族药物竹红菌的化学成分研究";郑立雄等;《云南中医学院学报》;20100630;第33卷(第3期);25-29 * |
| 郑立雄等."云南民族药物竹红菌的化学成分研究".《云南中医学院学报》.2010,第33卷(第3期),25-29. |
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