CN102405769A

CN102405769A - Industrial rapid culture technology of hypsizigus marmoreus

Info

Publication number: CN102405769A
Application number: CN2011102625704A
Authority: CN
Inventors: 盛岩; 赵磊; 李凤玲; 李波; 张金霞; 王艳霞; 蔡王涛; 董孝刚
Original assignee: SHANDONG ZHENGHAN BIOTECHNOLOGY GROUP CO Ltd
Current assignee: SHANDONG ZHENGHAN BIOTECHNOLOGY GROUP CO Ltd
Priority date: 2011-08-31
Filing date: 2011-08-31
Publication date: 2012-04-11

Abstract

The invention discloses an industrial rapid culture technology of hypsizigus marmoreus. The technology comprises a strain A and a strain B, wherein the strain A is a culture strain, and the strain B is a stock strain. The technology comprises the following steps: selecting the strain A with age of 50 to 80 days, and the strain B with age of 50 to 70 days, treating aged mycelia on the surface of the strain B, and inoculating the strain A; culturing for 7 to 15 days, and performing mycelium stimulation after the strain A and the strain B are combined; and after mycelium stimulation, placing a strain bottle into a planting room to perform fruiting cultivation. According to the technology, the growing period is shortened, the production cost is greatly reduced, the area of the planting room and the culturing energy can be saved, the bottle cap can be saved, regular fruiting effect is achieved and management can be carried out conveniently, and the mushroom cap has dark color, so that quality and yield of the hypsizigus marmoreus are improved.

Description

The quick culture technique of a kind of batch production Hypsizygus marmoreus

Technical field

The present invention relates to the quick culture technique of a kind of batch production Hypsizygus marmoreus.

Background technology

Conventional Hypsizygus marmoreus cultivation whole growth needs 105～130 days growth cycle, comprising sending out bacterium cultivation cycle 75～100 days, 20～30 days mushroom producing culture cycles.This is because under the cultivation mode of routine, and Hypsizygus marmoreus adopts solid spawn once to inoculate, and the time that inoculation back mycelia is covered with whole bacterium bottle is 35～50 days, and mycelia needs 35～55 days cultivation latter stage of ripening then, and whole bacterium cultivation cycle is 70～100 days.Send out bacterium and cultivate the end back entering mushroom producing culture cycle, gathering to fruit body also needs 20～30 days puberty, and wherein sending out the bacterium cultivation cycle long is the main factor of whole cultivation excessive cycle.The cell age of bacterial classification all has bigger influence to the whole growth cycle of Hypsizygus marmoreus.Cell age is too small, and then secondary metabolite such as cellulase etc. are less, and is lower with batch cultivated species cell age unification degree, and fruit body takes place and gathers asynchronously, influences output, has prolonged the whole production cycle of Hypsizygus marmoreus; Cell age is excessive, though can shorten the time in bacterial classification maturation stage, mycelia recovery and material feeding are slower, the control of pollution rate relative difficult.

Summary of the invention

The problem that the present invention is directed to the prior art existence provides a kind of batch production Hypsizygus marmoreus quick culture technique, and cultivation cycle shortens greatly, the fruiting regularity is higher, has practiced thrift production cost greatly, has improved the quality and yield of Hypsizygus marmoreus.

To achieve the above object, the present invention is a technical solution: A factory marmoreus rapid cultivation techniques, including the A and B strains strains, A strain was cultivated species, B strains of the original species; selected strain age of 50 to 80 days of the A strain, strain age of 50 to 70 days of B strains, the B strain of the old mycelium surface after processing, access A strain; Then after 7 to 15 days of training, to be A and B strains strains combined for Sao bacteria; finally Sao bottle into bacteria bacteria after birth cultured fruiting chamber.

In Hypsizygus marmoreus bacterial classification inoculation process, the A bacterial classification of bacterium bottle graft kind 15～25g of every bottle of 1100ml.

The beneficial effect that the present invention obtains is: through its cultivation cycle of this secondary inoculation technology shorten greatly, the fruiting regularity is higher; And this secondary inoculation technology is through the cell age of strict control A bacterial classification and the cell age of B bacterial classification; Thereby both best cell age section combinations have been confirmed; Through this combination, make the present invention have following characteristics:

1, shortened growth cycle, shortened at least 20 days, practiced thrift production cost greatly than once inoculating;

2, the energy is used in culturing room's area of saving 20% and cultivation;

3, the bottle cap of saving 20%;

4, fruiting is neat, is convenient to management;

5, mushroom lid color is darker, has improved the quality of Hypsizygus marmoreus;

6, improved output.

Embodiment

Embodiment one:

Select A strain of bacteria age of 70 days, B strains of bacteria strains age of 60 days, the surface of the B strain of the old mycelium after processing, 1100ml bottle access A strains cultivated around 15g, After 15 days of culture, to be A, B strains combined for Sao bacteria.After the Sao bacteria bottle bacteria cultured fruiting chamber into fertility.Give birth to cultivation after 20 days in fertility, gather.The whole production cycle is 95 days.

Embodiment two:

Select A strain of bacteria age of 80 days, B strains of bacteria strains age of 65 days, the surface of the B strain of the old mycelium after processing, 1100ml bottle cultivation access A strain 20g, by 10 days of culture, to be A, B strains combined for Sao bacteria.After the Sao bacteria bottle bacteria cultured fruiting chamber into fertility.Give birth to cultivation after 25 days in fertility, gather.The whole production cycle is 100 days.

Adopt the A+B secondary inoculation technology and the main distinction of once inoculation to be that its cultivation cycle shortens greatly, the fruiting regularity is higher.Since Hypsizygus marmoreus need be on the solid spawn of certain cell age, after-ripening fruiting, so the cell age of B bacterial classification and after-ripening degree are very important, cell age is too short, the after-ripening degree is not enough; Fruiting is few, otherwise cell age is long, after-ripening is excessive; Bacterial classification is dry, nutritional deficiency, and fruiting is few, influences output.Therefore, the cell age of B bacterial classification was advisable with 50～70 days.A+B secondary inoculation technology is controlled the cell age of A bacterial classification and the cell age of B bacterial classification through strict, thereby has confirmed both best cell age section combinations.

The present invention can shorten growth cycle, has practiced thrift production cost greatly; Can practice thrift culturing room's area and cultivate and to use the energy; Can practice thrift bottle cap; Fruiting is neat, is convenient to management; Mushroom lid color is darker, has improved the quality and yield of Hypsizygus marmoreus.

Claims

1 A factory marmoreus rapid cultivation techniques, wherein: including A and B strains strains, A strain was cultivated species, B strains of the original species; selected strain age of 50 to 80 days of the A strain, strain age of 50 to 70 days of B strains, the B strain of the old mycelium surface after processing, access A strain; Then after 7 to 15 days of culture, to be A and B bacteria strains species combined for Sao bacteria; finally Sao bottle into bacteria bacteria after birth cultured fruiting chamber.

2. the quick culture technique of a kind of batch production Hypsizygus marmoreus according to claim 1 is characterized in that: in Hypsizygus marmoreus bacterial classification inoculation process, and the A bacterial classification of bacterium bottle graft kind 15～25g of every bottle of 1100ml.