CN102399680B - 基于i型胶原凝胶颗粒制备与收集细胞微球的装置 - Google Patents
基于i型胶原凝胶颗粒制备与收集细胞微球的装置 Download PDFInfo
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- CN102399680B CN102399680B CN 201110377082 CN201110377082A CN102399680B CN 102399680 B CN102399680 B CN 102399680B CN 201110377082 CN201110377082 CN 201110377082 CN 201110377082 A CN201110377082 A CN 201110377082A CN 102399680 B CN102399680 B CN 102399680B
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- 102000012422 Collagen Type I Human genes 0.000 title claims abstract description 24
- 108010022452 Collagen Type I Proteins 0.000 title claims abstract description 24
- 239000000512 collagen gel Substances 0.000 title claims abstract description 24
- 239000002245 particle Substances 0.000 title claims abstract description 23
- 239000004005 microsphere Substances 0.000 title claims abstract description 21
- 230000003321 amplification Effects 0.000 claims abstract description 45
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 45
- 238000002360 preparation method Methods 0.000 claims abstract description 23
- 235000015097 nutrients Nutrition 0.000 claims description 77
- 238000012258 culturing Methods 0.000 claims description 36
- 230000005540 biological transmission Effects 0.000 claims description 34
- 238000007789 sealing Methods 0.000 claims description 33
- 238000004073 vulcanization Methods 0.000 claims description 18
- 239000000463 material Substances 0.000 claims description 16
- 230000001351 cycling effect Effects 0.000 claims description 15
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- 230000007306 turnover Effects 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 11
- 238000004114 suspension culture Methods 0.000 abstract description 3
- 239000012531 culture fluid Substances 0.000 abstract 4
- 230000007547 defect Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 121
- 238000011081 inoculation Methods 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 239000003814 drug Substances 0.000 description 3
- 230000001172 regenerating effect Effects 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 210000002744 extracellular matrix Anatomy 0.000 description 2
- 102000002734 Collagen Type VI Human genes 0.000 description 1
- 108010043741 Collagen Type VI Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
- C12M25/20—Fluidized bed
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
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Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN 201110377082 CN102399680B (zh) | 2011-11-24 | 2011-11-24 | 基于i型胶原凝胶颗粒制备与收集细胞微球的装置 |
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CN 201110377082 CN102399680B (zh) | 2011-11-24 | 2011-11-24 | 基于i型胶原凝胶颗粒制备与收集细胞微球的装置 |
Publications (2)
Publication Number | Publication Date |
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CN102399680A CN102399680A (zh) | 2012-04-04 |
CN102399680B true CN102399680B (zh) | 2013-05-22 |
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CN 201110377082 Expired - Fee Related CN102399680B (zh) | 2011-11-24 | 2011-11-24 | 基于i型胶原凝胶颗粒制备与收集细胞微球的装置 |
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Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
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KR101680181B1 (ko) * | 2008-07-16 | 2016-11-28 | 케이셉 시스템즈, 엘엘씨 | 유동층을 이용하여 입자들을 처리하는 방법 및 이를 위한 시스템 |
CN201785395U (zh) * | 2010-04-27 | 2011-04-06 | 惠识瑶 | 罐外循环的流化床式细胞反应器 |
CN202322811U (zh) * | 2011-11-24 | 2012-07-11 | 杭州电子科技大学 | 一种基于i型胶原凝胶颗粒制备与收集细胞微球的装置 |
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Effective date of registration: 20161008 Address after: 411101 high tech building, Furong Middle Road, high tech Zone, Hunan, Xiangtan, China, No. 9 Patentee after: Xiangtan poly energy saving environmental technology Co.,Ltd. Address before: Hangzhou City, Zhejiang province 310018 Xiasha Higher Education Park No. 2 street Patentee before: HANGZHOU DIANZI University |
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C41 | Transfer of patent application or patent right or utility model | ||
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Effective date of registration: 20161114 Address after: Hangzhou City, Zhejiang Province Economic Development Zone 310018 Poplar Street No. 6 Street No. 452 building 3 room 716 Patentee after: HANGZHOU BAIQIAO MEDICAL TECHNOLOGY CO.,LTD. Address before: 411101 high tech building, Furong Middle Road, high tech Zone, Hunan, Xiangtan, China, No. 9 Patentee before: Xiangtan poly energy saving environmental technology Co.,Ltd. |
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CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130522 |
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CF01 | Termination of patent right due to non-payment of annual fee |